amphibian proteins


Summary: Proteins obtained from species in the class of AMPHIBIANS.

Top Publications

  1. Campbell L, Suárez Castillo E, Ortiz Zuazaga H, Knapp D, Tanaka E, Crews C. Gene expression profile of the regeneration epithelium during axolotl limb regeneration. Dev Dyn. 2011;240:1826-40 pubmed publisher
    ..A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE...
  2. Zairi A, Belaid A, Gahbiche A, Hani K. Spermicidal activity of dermaseptins. Contraception. 2005;72:447-53 pubmed
    ..In view of this fact, it is suggested that DS4 has antibacterial, antiviral, antifungal and potentially spermicidal activities and could be a potent vaginal contraceptive. ..
  3. Niidome T, Kobayashi K, Arakawa H, Hatakeyama T, Aoyagi H. Structure-activity relationship of an antibacterial peptide, maculatin 1.1, from the skin glands of the tree frog, Litoria genimaculata. J Pept Sci. 2004;10:414-22 pubmed
    ..These results indicate that the entire chain length of Mac is necessary for full activity, and the basicity of the peptides greatly affects the activity. ..
  4. Seto G, Marwaha S, Kobewka D, Lewis R, Separovic F, McElhaney R. Interactions of the Australian tree frog antimicrobial peptides aurein 1.2, citropin 1.1 and maculatin 1.1 with lipid model membranes: differential scanning calorimetric and Fourier transform infrared spectroscopic studies. Biochim Biophys Acta. 2007;1768:2787-800 pubmed
    ..2 is more disruptive of model lipid membranes than citropin 1.1 and exhibits comparable antimicrobial activity, probably because aurein 1.2 has a higher propensity for partitioning into phospholipid membranes...
  5. Bozzi A, Mangoni M, Rinaldi A, Mignogna G, Aschi M. Folding propensity and biological activity of peptides: the effect of a single stereochemical isomerization on the conformational properties of bombinins in aqueous solution. Biopolymers. 2008;89:769-78 pubmed publisher
    ..e. interaction with membrane, supporting the hypothesis of an "already folded" conformation in water rather than interfacial folding tenet. ..
  6. Ambroggio E, Separovic F, Bowie J, Fidelio G, Bagatolli L. Direct visualization of membrane leakage induced by the antibiotic peptides: maculatin, citropin, and aurein. Biophys J. 2005;89:1874-81 pubmed
    ..As observed for maculatin, the lytic action of Maculatin-Ala and maculatin-Gly is in keeping with the formation of pore-like structures at the membrane independently of lipid composition. ..
  7. Nicolas P, El Amri C. The dermaseptin superfamily: a gene-based combinatorial library of antimicrobial peptides. Biochim Biophys Acta. 2009;1788:1537-50 pubmed publisher
  8. Castro M, Ferreira T, Cilli E, Crusca E, Mendes Giannini M, Sebben A, et al. Hylin a1, the first cytolytic peptide isolated from the arboreal South American frog Hypsiboas albopunctatus ("spotted treefrog"). Peptides. 2009;30:291-6 pubmed publisher
    ..Similarity analysis using PSI-BLAST revealed 50-44% of identity to maximins Hv, H16, H15 and H10 from Bombina maxima and also to hylins b1 and b2 (Hy-b1 and Hy-b2) from Hypsiboas lundii (synonym: Hyla biobeba). ..
  9. Joanne P, Galanth C, Goasdoue N, Nicolas P, Sagan S, Lavielle S, et al. Lipid reorganization induced by membrane-active peptides probed using differential scanning calorimetry. Biochim Biophys Acta. 2009;1788:1772-81 pubmed publisher
    ..Moreover, due to the distinct membrane composition of prokaryotes and eukaryotes, lateral heterogeneity may be differently affected by cationic peptides leading to either uptake or/and antimicrobial activities. ..

More Information


  1. Amiche M, Galanth C. Dermaseptins as models for the elucidation of membrane-acting helical amphipathic antimicrobial peptides. Curr Pharm Biotechnol. 2011;12:1184-93 pubmed
    ..These peptides could be good models to study the membrane-peptide interactions discussed in this review. ..
  2. Kiemnec Tyburczy K, Richmond J, Savage A, Lips K, Zamudio K. Genetic diversity of MHC class I loci in six non-model frogs is shaped by positive selection and gene duplication. Heredity (Edinb). 2012;109:146-55 pubmed publisher
    ..Thus, the accumulation of genetic data for more species will be useful in further characterizing the relative importance of processes such as selection, recombination and gene duplication in shaping MHC loci among amphibian lineages...
  3. Zairi A, Serres C, Tangy F, Jouannet P, Hani K. In vitro spermicidal activity of peptides from amphibian skin: dermaseptin S4 and derivatives. Bioorg Med Chem. 2008;16:266-75 pubmed
    ..This study indicates that dermaseptins are spermicidal molecules that deserve to be tested as topical contraceptive with useful activities that can add to their prophylaxis, safety, and effectiveness. ..
  4. Chinchar V, Bryan L, Silphadaung U, Noga E, Wade D, Rollins Smith L. Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. Virology. 2004;323:268-75 pubmed
    ..These results extend our previous findings and strongly suggest that various amphibian and piscine AMPs may play important roles in protecting fish and amphibians from pathogenic viruses...
  5. Ambroggio E, Separovic F, Bowie J, Fidelio G. Surface behaviour and peptide-lipid interactions of the antibiotic peptides, Maculatin and Citropin. Biochim Biophys Acta. 2004;1664:31-7 pubmed
    ..Additional penetration experiments also demonstrated that both peptides better interact with POPG compared with POPC monolayers. This lipid preference is discussed as a possible explanation of their antibiotic properties. ..
  6. Zairi A, Tangy F, Saadi S, Hani K. In vitro activity of dermaseptin S4 derivatives against genital infections pathogens. Regul Toxicol Pharmacol. 2008;50:353-8 pubmed publisher
    ..To assess the structure-activity relationship between the native DS4 and their derivatives, a set of chemically modified peptides was synthesized and evaluated...
  7. Lorin C, Saidi H, Belaid A, Zairi A, Baleux F, Hocini H, et al. The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro. Virology. 2005;334:264-75 pubmed
    ..The modified DS4 inhibited HIV-1 capture by dendritic cells and subsequent transmission to CD4(+) T cells, as well as HIV-1 binding on HEC-1 endometrial cells and transcytosis through a tight epithelial monolayer. ..
  8. Amiche M, Ladram A, Nicolas P. A consistent nomenclature of antimicrobial peptides isolated from frogs of the subfamily Phyllomedusinae. Peptides. 2008;29:2074-82 pubmed publisher
    ..For example, the abbreviation DI for distincta, DU for duellmani, VA for vaillanti and VN for vanzolinii. Paralogous peptides should bear letter(s) in upper case followed by numbers. ..
  9. Vanhoye D, Bruston F, Nicolas P, Amiche M. Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain. Eur J Biochem. 2003;270:2068-81 pubmed
  10. Conlon J, Galadari S, Raza H, Condamine E. Design of potent, non-toxic antimicrobial agents based upon the naturally occurring frog skin peptides, ascaphin-8 and peptide XT-7. Chem Biol Drug Des. 2008;72:58-64 pubmed publisher
  11. Rydlo T, Rotem S, Mor A. Antibacterial properties of dermaseptin S4 derivatives under extreme incubation conditions. Antimicrob Agents Chemother. 2006;50:490-7 pubmed
    ..Collectively, the data indicate interest in simple peptide-based compounds for design of antimicrobials that affect pathogens under a variable range of incubation conditions. ..
  12. Chen T, Zhou M, Gagliardo R, Walker B, Shaw C. Elements of the granular gland peptidome and transcriptome persist in air-dried skin of the South American orange-legged leaf frog, Phyllomedusa hypocondrialis. Peptides. 2006;27:2129-36 pubmed
  13. Abbassi F, Raja Z, Oury B, Gazanion E, Piesse C, Sereno D, et al. Antibacterial and leishmanicidal activities of temporin-SHd, a 17-residue long membrane-damaging peptide. Biochimie. 2013;95:388-99 pubmed publisher
  14. Rosenfeld Y, Barra D, Simmaco M, Shai Y, Mangoni M. A synergism between temporins toward Gram-negative bacteria overcomes resistance imposed by the lipopolysaccharide protective layer. J Biol Chem. 2006;281:28565-74 pubmed
    ..To the best of our knowledge, this is the first study that explains how a combination of native AMPs from the same species can overcome bacterial resistance imposed by the LPS leaflet...
  15. Conlon J, Woodhams D, Raza H, Coquet L, Leprince J, Jouenne T, et al. Peptides with differential cytolytic activity from skin secretions of the lemur leaf frog Hylomantis lemur (Hylidae: Phyllomedusinae). Toxicon. 2007;50:498-506 pubmed
  16. Tang C, Hu C, Wei C, Wang J. Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells. FEBS Lett. 2005;579:265-70 pubmed
    ..Our findings provide evidence that RC-RNase and RC-RNase/IFN-gamma can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity. ..
  17. Bond P, Parton D, Clark J, Sansom M. Coarse-grained simulations of the membrane-active antimicrobial Peptide maculatin 1.1. Biophys J. 2008;95:3802-15 pubmed publisher
    ..This contrasted with the behavior of the nonpore-forming model peptide WALP23, which inserted into the vesicle to form extended clusters of TM alpha-helices with relatively little perturbation of bilayer properties. ..
  18. Rollins Smith L. The role of amphibian antimicrobial peptides in protection of amphibians from pathogens linked to global amphibian declines. Biochim Biophys Acta. 2009;1788:1593-9 pubmed publisher
    ..However, this defense must also accommodate potentially beneficial microbes on the skin that compete or inhibit growth of the pathogens. How this delicate balancing act is accomplished is an important area of future research. ..
  19. Crusca E, Rezende A, Marchetto R, Mendes Giannini M, Fontes W, Castro M, et al. Influence of N-terminus modifications on the biological activity, membrane interaction, and secondary structure of the antimicrobial peptide hylin-a1. Biopolymers. 2011;96:41-8 pubmed publisher
    ..However, these data have no relationship with activity against Gram-positive bacteria. These results showed that the N-terminal region of the peptide Hy-a1 has key roles in its antibacterial action toward different types of bacteria. ..
  20. Lequin O, Bruston F, Convert O, Chassaing G, Nicolas P. Helical structure of dermaseptin B2 in a membrane-mimetic environment. Biochemistry. 2003;42:10311-23 pubmed
    ..Structure-activity studies on dermaseptin B2 analogues showed that the N-terminal 1-11 segment is an absolute requirement for antibacterial activity, while the C-terminal 10-33 region is also important for full antibiotic activity. ..
  21. Wegener K, Carver J, Bowie J. The solution structures and activity of caerin 1.1 and caerin 1.4 in aqueous trifluoroethanol and dodecylphosphocholine micelles. Biopolymers. 2003;69:42-59 pubmed
    ..The similarity of the structures calculated in aqueous trifluoroethanol and dodecylphosphocholine micelles suggests that, for caerin 1.1 and 1.4, these solvent systems are equally as good at representing a membrane environment. ..
  22. Galanth C, Abbassi F, Lequin O, Ayala Sanmartin J, Ladram A, Nicolas P, et al. Mechanism of antibacterial action of dermaseptin B2: interplay between helix-hinge-helix structure and membrane curvature strain. Biochemistry. 2009;48:313-27 pubmed publisher
  23. Gössler Schöfberger R, Hesser G, Muik M, Wechselberger C, Jilek A. An orphan dermaseptin from frog skin reversibly assembles to amyloid-like aggregates in a pH-dependent fashion. FEBS J. 2009;276:5849-59 pubmed publisher
  24. Zairi A, Tangy F, Ducos Galand M, Alonso J, Hani K. Susceptibility of Neisseria gonorrhoeae to antimicrobial peptides from amphibian skin, dermaseptin, and derivatives. Diagn Microbiol Infect Dis. 2007;57:319-24 pubmed
    ..Among the different molecules tested, we found that dermaseptins K4S4 (1-16)a and K4S4 (1-28) were the more potent to inhibit N. gonorrhoeae growth with MIC of 10 microg/mL against all strains. ..
  25. Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni M, Barra D. Temporins, antimicrobial peptides from the European red frog Rana temporaria. Eur J Biochem. 1996;242:788-92 pubmed
    ..1984) J. Biol. Chem. 259, 10106-10111]. Natural and synthetic temporins have antibacterial activity against gram-positive bacteria, but they are not hemolytic. Temporins are the smallest antibacterial peptides hitherto found in nature. ..
  26. Daly J, Caceres J, Moni R, Gusovsky F, Moos M, Seamon K, et al. Frog secretions and hunting magic in the upper Amazon: identification of a peptide that interacts with an adenosine receptor. Proc Natl Acad Sci U S A. 1992;89:10960-3 pubmed
    ..The vasoactive peptide sauvagine, the opioid peptides, and adenoregulin and related peptides affect behavior in mice and presumably contribute to the behavioral sequelae observed in humans. ..
  27. Duda T, Vanhoye D, Nicolas P. Roles of diversifying selection and coordinated evolution in the evolution of amphibian antimicrobial peptides. Mol Biol Evol. 2002;19:858-64 pubmed
  28. Efron L, Dagan A, Gaidukov L, Ginsburg H, Mor A. Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture. J Biol Chem. 2002;277:24067-72 pubmed
  29. Dagan A, Efron L, Gaidukov L, Mor A, Ginsburg H. In vitro antiplasmodium effects of dermaseptin S4 derivatives. Antimicrob Agents Chemother. 2002;46:1059-66 pubmed
    ..Overall, the data indicate the potential usefulness of this strategy for development of selective peptides as investigative tools and eventually as antimalarial agents. ..
  30. Kustanovich I, Shalev D, Mikhlin M, Gaidukov L, Mor A. Structural requirements for potent versus selective cytotoxicity for antimicrobial dermaseptin S4 derivatives. J Biol Chem. 2002;277:16941-51 pubmed
    ..The usefulness of this approach for the design of potent and/or selective cytolytic peptides is discussed herein. ..
  31. Chia C, Torres J, Cooper M, Arkin I, Bowie J. The orientation of the antibiotic peptide maculatin 1.1 in DMPG and DMPC lipid bilayers. Support for a pore-forming mechanism. FEBS Lett. 2002;512:47-51 pubmed
  32. Shai Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim Biophys Acta. 1999;1462:55-70 pubmed
  33. Lacombe C, Cifuentes Diaz C, Dunia I, Auber Thomay M, Nicolas P, Amiche M. Peptide secretion in the cutaneous glands of South American tree frog Phyllomedusa bicolor: an ultrastructural study. Eur J Cell Biol. 2000;79:631-41 pubmed
    ..Scanning and transmission electron microscopy revealed that the serous glands of P bicolor have morphological features, especially the secretory granules, which differ from those of the glands in Xenopus laevis skin. ..
  34. Chinchar V, Wang J, Murti G, Carey C, Rollins Smith L. Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin. Virology. 2001;288:351-7 pubmed
    ..This is the first report linking amphibian antimicrobial peptides with protection from an amphibian viral pathogen and suggests that these compounds may play a role in maintaining amphibian health. ..
  35. Wegener K, Wabnitz P, Carver J, Bowie J, Chia B, Wallace J, et al. Host defence peptides from the skin glands of the Australian blue mountains tree-frog Litoria citropa. Solution structure of the antibacterial peptide citropin 1.1. Eur J Biochem. 1999;265:627-37 pubmed
    ..g. citropin 1.1.3 (GLFDVIKKVASVIGLASP-OH)]. These peptides show minimal antibacterial activity; their role in the amphibian skin is not known. ..
  36. Navon Venezia S, Feder R, Gaidukov L, Carmeli Y, Mor A. Antibacterial properties of dermaseptin S4 derivatives with in vivo activity. Antimicrob Agents Chemother. 2002;46:689-94 pubmed
    ..Overall, the data suggest that dermaseptin S4 derivatives could be useful in treatment of infections, including infections caused by multidrug-resistant bacteria. ..
  37. Coote P, Holyoak C, Bracey D, Ferdinando D, Pearce J. Inhibitory action of a truncated derivative of the amphibian skin peptide dermaseptin s3 on Saccharomyces cerevisiae. Antimicrob Agents Chemother. 1998;42:2160-70 pubmed
    ..In summary, a derivative of a natural antimicrobial peptide has potential, either alone or in combination with mild heating, to prevent the growth of or kill spoilage yeast. ..
  38. Reconditi M, Brunello E, Linari M, Bianco P, Narayanan T, Panine P, et al. Motion of myosin head domains during activation and force development in skeletal muscle. Proc Natl Acad Sci U S A. 2011;108:7236-40 pubmed publisher
  39. Million M, Grigoriadis D, Sullivan S, Crowe P, McRoberts J, Zhou H, et al. A novel water-soluble selective CRF1 receptor antagonist, NBI 35965, blunts stress-induced visceral hyperalgesia and colonic motor function in rats. Brain Res. 2003;985:32-42 pubmed
  40. Shukolyukov S. Aggregation of frog rhodopsin to oligomers and their dissociation to monomer: application of BN- and SDS-PAGE. Biochemistry (Mosc). 2009;74:599-604 pubmed
    ..After solubilization with denaturing detergents, RhA can dissociate into monomers that then spontaneously self-associate into higher oligomers under the influence of various factors (for example, heating). ..
  41. Arch V, Simmons D, Quiñones P, Feng A, Jiang J, Stuart B, et al. Inner ear morphological correlates of ultrasonic hearing in frogs. Hear Res. 2012;283:70-9 pubmed publisher
    ..chloronota, suggesting that this species is a candidate for high-frequency hearing sensitivity. These data form the foundation for future functional work probing the physiological bases of ultrasound detection by a non-mammalian ear. ..
  42. Bowie J, Separovic F, Tyler M. Host-defense peptides of Australian anurans. Part 2. Structure, activity, mechanism of action, and evolutionary significance. Peptides. 2012;37:174-88 pubmed publisher
    ..The review discusses the outcome of cDNA sequencing of signal-spacer-active peptides from an evolutionary viewpoint, and also lists those peptides for which activities have not been found to this time. ..
  43. Wang R, Chen T, Zhou M, Wang L, Shaw C. PsT-1: a new tryptophyllin peptide from the skin secretion of Waxy Monkey Leaf Frog, Phyllomedusa sauvagei. Regul Pept. 2013;184:14-21 pubmed publisher
  44. Conlon J, Abraham B, Galadari S, Knoop F, Sonnevend A, Pal T. Antimicrobial and cytolytic properties of the frog skin peptide, kassinatuerin-1 and its L- and D-lysine-substituted derivatives. Peptides. 2005;26:2104-10 pubmed
    ..However, the reduction in alpha-helicity produced by the D-amino acid substitutions resulted in analogs with reduced potencies against Gram-positive bacteria and against C. albicans. ..
  45. Doyle J, Brinkworth C, Wegener K, Carver J, Llewellyn L, Olver I, et al. nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications. The solution structure of a modified citropin 1.1. Eur J Biochem. 2003;270:1141-53 pubmed
    ..The structure of one of the more active synthetic modifications (A4K14-citropin 1.1) was determined in aqueous trifluoroethanol, showing that this peptide also forms an amphipathic alpha-helix. ..
  46. Facci L, Stevens D, Pangallo M, Franceschini D, Skaper S, Strijbos P. Corticotropin-releasing factor (CRF) and related peptides confer neuroprotection via type 1 CRF receptors. Neuropharmacology. 2003;45:623-36 pubmed
    ..Taken together, these results suggest that the neuroprotective activity of CRF may involve cAMP-dependent phosphorylation of glycogen synthase kinase-3. ..
  47. de Moraes J, Nascimento C, Miura L, Leite J, Nakano E, Kawano T. Evaluation of the in vitro activity of dermaseptin 01, a cationic antimicrobial peptide, against Schistosoma mansoni. Chem Biodivers. 2011;8:548-58 pubmed publisher
    ..It was the first time that an anthelmintic activity towards schistosomes has been reported for a dermaseptin. ..
  48. Tennessen J, Woodhams D, Chaurand P, Reinert L, Billheimer D, Shyr Y, et al. Variations in the expressed antimicrobial peptide repertoire of northern leopard frog (Rana pipiens) populations suggest intraspecies differences in resistance to pathogens. Dev Comp Immunol. 2009;33:1247-57 pubmed publisher
  49. Wang L, Zhou M, McGrath S, Chen T, Gorman S, Walker B, et al. A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata. Peptides. 2009;30:1428-33 pubmed publisher
    ..The kassinatuerin-2 related peptides of K. maculata skin secretion thus possess a discrete antimicrobial and weak haemolytic activity in contrast to the prototype kassinatuerin-2 from the skin secretion of Kassina senegalensis. ..
  50. Cornelisse L, Deumens R, Coenen J, Roubos E, Gielen C, Ypey D, et al. Sauvagine regulates Ca2+ oscillations and electrical membrane activity of melanotrope cells of Xenopus laevis. J Neuroendocrinol. 2002;14:778-87 pubmed
  51. Abed M, Zoubi K, Theurer M, Lang F. Effect of dermaseptin on erythrocytes. Basic Clin Pharmacol Toxicol. 2013;113:347-52 pubmed publisher
    ..Dermaseptin triggers eryptosis, an effect at least partially due to entry of extracellular Ca²?. ..
  52. Li X, Feng W, Zhou M, Ma C, Chen T, Zeller M, et al. Kasstasin: A novel potent vasoconstrictor peptide from the skin secretion of the African red-legged running frog, Kassina maculata. Biochimie. 2011;93:1537-42 pubmed publisher
    ..These data illustrate the continuing potential of amphibian skin secretions to provide novel natural peptide templates for biological evaluation...
  53. Wei C, Hu C, Tang C, Lee M, Wang J. Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death. FEBS Lett. 2002;531:421-6 pubmed
    ..Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway. ..