lactalbumin

Summary

Summary: A major protein fraction of milk obtained from the WHEY.

Top Publications

  1. Palmer D, Kelly V, Smit A, Kuy S, Knight C, Cooper G. Human colostrum: identification of minor proteins in the aqueous phase by proteomics. Proteomics. 2006;6:2208-16 pubmed
    ..the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods...
  2. Marks L, Clementi E, Hakansson A. The human milk protein-lipid complex HAMLET sensitizes bacterial pathogens to traditional antimicrobial agents. PLoS ONE. 2012;7:e43514 pubmed publisher
    ..Combined these results suggest the presence of a conserved HAMLET-activated pathway that circumvents antibiotic resistance in bacteria. The ability to activate this pathway may extend the lifetime of the current treatment arsenal. ..
  3. Bruylants G, Redfield C. (15)N NMR relaxation data reveal significant chemical exchange broadening in the alpha-domain of human alpha-lactalbumin. Biochemistry. 2009;48:4031-9 pubmed publisher
    Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues...
  4. Zherelova O, Kataev A, Grishchenko V, Knyazeva E, Permyakov S, Permyakov E. Interaction of antitumor alpha-lactalbumin-oleic acid complexes with artificial and natural membranes. J Bioenerg Biomembr. 2009;41:229-37 pubmed publisher
    The specific complexes of human alpha-lactalbumin (alpha-LA) with oleic acid (OA), HAMLET and LA-OA-17 (OA-complexes), possess cytotoxic activity against tumor cells but the mechanism of their cell penetration remains unclear...
  5. Schopen G, Visker M, Koks P, Mullaart E, van Arendonk J, Bovenhuis H. Whole-genome association study for milk protein composition in dairy cattle. J Dairy Sci. 2011;94:3148-58 pubmed publisher
    ..content in bovine milk of ?(S1)-casein (?(S1)-CN), ?(S2)-casein (?(S2)-CN), ?-casein (?-CN), ?-casein (?-CN), ?-lactalbumin (?-LA), ?-lactoglobulin (?-LG), casein index, protein percentage, and protein yield using a 50K single ..
  6. Rösner H, Redfield C. The human alpha-lactalbumin molten globule: comparison of structural preferences at pH 2 and pH 7. J Mol Biol. 2009;394:351-62 pubmed publisher
    ..For human alpha-lactalbumin (alpha-LA), the equilibrium molten globule is most often studied at pH 2, the so-called A-state, while kinetic ..
  7. Veldhorst M, Nieuwenhuizen A, Hochstenbach Waelen A, Westerterp K, Engelen M, Brummer R, et al. A breakfast with alpha-lactalbumin, gelatin, or gelatin + TRP lowers energy intake at lunch compared with a breakfast with casein, soy, whey, or whey-GMP. Clin Nutr. 2009;28:147-55 pubmed publisher
    ..The objective was to compare the effects of casein-, soy-, whey-, whey without glycomacropeptide (GMP)-, alpha-lactalbumin-, gelatin-, or gelatin with tryptophan (TRP)-protein breakfasts at two concentrations on subsequent satiety and ..
  8. Li X, Zhang J, Gao H, Vieth E, Bae K, Zhang Y, et al. Transcriptional targeting modalities in breast cancer gene therapy using adenovirus vectors controlled by alpha-lactalbumin promoter. Mol Cancer Ther. 2005;4:1850-9 pubmed
    The breast-specific antigen alpha-lactalbumin is expressed in >60% of breast cancer tissues...
  9. Bleck G, Wheeler M, Hansen L, Chester Jones H, Miller D. Lactose synthase components in milk: concentrations of alpha-lactalbumin and beta1,4-galactosyltransferase in milk of cows from several breeds at various stages of lactation. Reprod Domest Anim. 2009;44:241-7 pubmed publisher
    ..this study was to determine whether the concentrations in milk of the two components of lactose synthase, alpha-lactalbumin (alpha-LA) and beta1,4-galactosyltransferase (B4GALT), were related to genetic background, stage of lactation, ..

More Information

Publications62

  1. Hallgren O, Aits S, Brest P, Gustafsson L, Mossberg A, Wullt B, et al. Apoptosis and tumor cell death in response to HAMLET (human alpha-lactalbumin made lethal to tumor cells). Adv Exp Med Biol. 2008;606:217-40 pubmed publisher
    HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a molecular complex derived from human milk that kills tumor cells by a process resembling programmed cell death...
  2. Brest P, Gustafsson M, Mossberg A, Gustafsson L, Duringer C, Hamiche A, et al. Histone deacetylase inhibitors promote the tumoricidal effect of HAMLET. Cancer Res. 2007;67:11327-34 pubmed
    Histone deacetylase inhibitors (HDIs) and HAMLET (human alpha-lactalbumin made lethal to tumor cells) interact with histones, modify the structure of chromatin, and trigger tumor cell death...
  3. Verschoor E, Finlayson G, Blundell J, Markus C, King N. Effects of an acute alpha-lactalbumin manipulation on mood and food hedonics in high- and low-trait anxiety individuals. Br J Nutr. 2010;104:595-602 pubmed publisher
    ..Participants were provided with 40 g alpha-lactalbumin (LAC; l-tryptophan (Trp):large neutral amino acids (LNAA) ratio of 7...
  4. Baumann A, Gjerde A, Ying M, Svanborg C, Holmsen H, Glomm W, et al. HAMLET forms annular oligomers when deposited with phospholipid monolayers. J Mol Biol. 2012;418:90-102 pubmed publisher
    Recently, the anticancer activity of human ?-lactalbumin made lethal to tumor cells (HAMLET) has been linked to its increased membrane affinity in vitro, at neutral pH, and ability to cause leakage relative to the inactive native bovine ?..
  5. Zhang D, Wu L, Chen J, Liang Y. Effects of macromolecular crowding on the structural stability of human ?-lactalbumin. Acta Biochim Biophys Sin (Shanghai). 2012;44:703-11 pubmed publisher
    ..b>Lactalbumin, as a model system for protein-folding studies, has been used extensively because it can form stable molten ..
  6. Tolin S, De Franceschi G, Spolaore B, Frare E, Canton M, Polverino De Laureto P, et al. The oleic acid complexes of proteolytic fragments of alpha-lactalbumin display apoptotic activity. FEBS J. 2010;277:163-73 pubmed publisher
    The complexes formed by partially folded human and bovine alpha-lactalbumin with oleic acid (OA) have been reported to display selective apoptotic activity against tumor cells...
  7. Mossberg A, Hun Mok K, Morozova Roche L, Svanborg C. Structure and function of human ?-lactalbumin made lethal to tumor cells (HAMLET)-type complexes. FEBS J. 2010;277:4614-25 pubmed publisher
    Human ?-lactalbumin made lethal to tumor cells (HAMLET) and equine lysozyme with oleic acid (ELOA) are complexes consisting of protein and fatty acid that exhibit cytotoxic activities, drastically differing from the activity of their ..
  8. Heijnis W, Dekker H, de Koning L, Wierenga P, Westphal A, de Koster C, et al. Identification of the peroxidase-generated intermolecular dityrosine cross-link in bovine ?-lactalbumin. J Agric Food Chem. 2011;59:444-9 pubmed publisher
    The peroxidase-mediated oxidation of calcium-depleted bovine ?-lactalbumin generates a mixture of covalently bound protein oligomers with interesting foaming properties...
  9. Halskau O, Perez Jimenez R, Ibarra Molero B, Underhaug J, MUNOZ V, Martinez A, et al. Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics. Proc Natl Acad Sci U S A. 2008;105:8625-30 pubmed publisher
    ..the thermodynamic folding barriers of two relatively large proteins of the same size and topology: bovine alpha-lactalbumin (BLA) and hen-egg-white lysozyme (HEWL)...
  10. Ramboarina S, Redfield C. Probing the effect of temperature on the backbone dynamics of the human alpha-lactalbumin molten globule. J Am Chem Soc. 2008;130:15318-26 pubmed publisher
    ..Human alpha-lactalbumin (alpha-LA) is a two-domain Ca(2+)-binding protein that partially unfolds at low pH to form a molten globule...
  11. Bomhoff G, Sloan K, McLain C, Gogol E, Fisher M. The effects of the flavonoid baicalein and osmolytes on the Mg 2+ accelerated aggregation/fibrillation of carboxymethylated bovine 1SS-alpha-lactalbumin. Arch Biochem Biophys. 2006;453:75-86 pubmed
    ..Given this similarity, we tested the notion that carboxymethylated bovine alpha-lactalbumin (1SS-alpha-lac) could serve as a general amyloid fibrillation/aggregation model system...
  12. Heijnis W, Wierenga P, van Berkel W, Gruppen H. Directing the oligomer size distribution of peroxidase-mediated cross-linked bovine alpha-lactalbumin. J Agric Food Chem. 2010;58:5692-7 pubmed publisher
    ..the optimization of the horseradish peroxidase-mediated cross-linking of calcium-depleted bovine alpha-lactalbumin. To characterize the formed products, the molecular weight distribution of the cross-linked protein was ..
  13. Regini J, Ecroyd H, Meehan S, Bremmell K, Clarke M, Lammie D, et al. The interaction of unfolding ?-lactalbumin and malate dehydrogenase with the molecular chaperone ?B-crystallin: a light and X-ray scattering investigation. Mol Vis. 2010;16:2446-56 pubmed
    ..e., reduction of ?-lactalbumin at 37 °C and malate dehydrogenase when heated at 42 °C...
  14. Hakansson A, Roche Hakansson H, Mossberg A, Svanborg C. Apoptosis-like death in bacteria induced by HAMLET, a human milk lipid-protein complex. PLoS ONE. 2011;6:e17717 pubmed publisher
    ..compared the characteristics of tumor and bacterial cell death induced by HAMLET, a human milk complex of alpha-lactalbumin and oleic acid...
  15. Wilhelm K, Darinskas A, Noppe W, Duchardt E, Mok K, Vukojevic V, et al. Protein oligomerization induced by oleic acid at the solid-liquid interface--equine lysozyme cytotoxic complexes. FEBS J. 2009;276:3975-89 pubmed publisher
    ..with both amyloid oligomers and the complexes with oleic acid and its structural homologous protein alpha-lactalbumin, known as human alpha-lactalbumin made lethal for tumour cells (HAMLET)...
  16. Mossberg A, Wullt B, Gustafsson L, Mansson W, Ljunggren E, Svanborg C. Bladder cancers respond to intravesical instillation of HAMLET (human alpha-lactalbumin made lethal to tumor cells). Int J Cancer. 2007;121:1352-9 pubmed
    We studied if bladder cancers respond to HAMLET (human alpha-lactalbumin made lethal to tumor cells) to establish if intravesical HAMLET application might be used to selectively remove cancer cells in vivo...
  17. Oroguchi T, Ikeguchi M, Ota M, Kuwajima K, Kidera A. Unfolding pathways of goat alpha-lactalbumin as revealed in multiple alignment of molecular dynamics trajectories. J Mol Biol. 2007;371:1354-64 pubmed
    ..unfolding were performed at an elevated temperature for the authentic and recombinant forms of goat alpha-lactalbumin. Despite very similar three-dimensional structures, the two forms have significantly different unfolding rates ..
  18. Schlepckow K, Wirmer J, Bachmann A, Kiefhaber T, Schwalbe H. Conserved folding pathways of alpha-lactalbumin and lysozyme revealed by kinetic CD, fluorescence, NMR, and interrupted refolding experiments. J Mol Biol. 2008;378:686-98 pubmed publisher
    ..stopped-flow CD, fluorescence, and time-resolved NMR studies that the Ca(2+)-induced refolding of bovine alpha-lactalbumin (BLA) at constant denaturant concentration (4 M urea) exhibits triple-exponential kinetics...
  19. Bu G, Luo Y, Zhang Y, Chen F. Effects of fermentation by lactic acid bacteria on the antigenicity of bovine whey proteins. J Sci Food Agric. 2010;90:2015-20 pubmed publisher
    The main whey proteins alpha-lactalbumin (alpha-LA) and beta-lactoglobulin (beta-LG) are considered as the major allergens in cow's milk...
  20. Nakamura T, Aizawa T, Kariya R, Okada S, Demura M, Kawano K, et al. Molecular mechanisms of the cytotoxicity of human ?-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes. J Biol Chem. 2013;288:14408-16 pubmed publisher
    Although HAMLET (human ?-lactalbumin made lethal to tumor cells), a complex formed by human ?-lactalbumin and oleic acid, has a unique apoptotic activity for the selective killing of tumor cells, the molecular mechanisms of expression of ..
  21. Storm P, Klausen T, Trulsson M, Ho C S J, Dosnon M, Westergren T, et al. A unifying mechanism for cancer cell death through ion channel activation by HAMLET. PLoS ONE. 2013;8:e58578 pubmed publisher
    ..The results also suggest that HAMLET offers a two-tiered therapeutic approach, killing cancer cells while stimulating an innate immune response in surrounding healthy tissues. ..
  22. Mercer N, Ramakrishnan B, Boeggeman E, Qasba P. Applications of site-specific labeling to study HAMLET, a tumoricidal complex of ?-lactalbumin and oleic acid. PLoS ONE. 2011;6:e26093 pubmed publisher
    Alpha-lactalbumin (?-LA) is a calcium-bound mammary gland-specific protein that is found in milk...
  23. Kamijima T, Ohmura A, Sato T, Akimoto K, Itabashi M, Mizuguchi M, et al. Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death. Biochem Biophys Res Commun. 2008;376:211-4 pubmed publisher
    HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells), which was identified in human breast milk as an alpha-lactalbumin (LA)-oleic acid complex, kills tumor cells, selectively...
  24. Otzen D, Sehgal P, Westh P. Alpha-Lactalbumin is unfolded by all classes of surfactants but by different mechanisms. J Colloid Interface Sci. 2009;329:273-83 pubmed publisher
    We show that all four classes of surfactants (anionic, cationic, non-ionic, and zwitterionic) denature alpha-lactalbumin (alphaLA), making alphaLA an excellent model system to compare their denaturation mechanisms...
  25. Aits S, Gustafsson L, Hallgren O, Brest P, Gustafsson M, Trulsson M, et al. HAMLET (human alpha-lactalbumin made lethal to tumor cells) triggers autophagic tumor cell death. Int J Cancer. 2009;124:1008-19 pubmed publisher
    HAMLET, a complex of partially unfolded alpha-lactalbumin and oleic acid, kills a wide range of tumor cells. Here we propose that HAMLET causes macroautophagy in tumor cells and that this contributes to their death...
  26. Permyakov S, Knyazeva E, Khasanova L, Fadeev R, Zhadan A, Roche Hakansson H, et al. Oleic acid is a key cytotoxic component of HAMLET-like complexes. Biol Chem. 2012;393:85-92 pubmed publisher
    HAMLET is a complex of ?-lactalbumin (?-LA) with oleic acid (OA) that selectively kills tumor cells and Streptococcus pneumoniae...
  27. Fogle J, O Connell J, Fernandez E. Loading, stationary phase, and salt effects during hydrophobic interaction chromatography: alpha-lactalbumin is stabilized at high loadings. J Chromatogr A. 2006;1121:209-18 pubmed
    ..hydrogen-deuterium exchange labeling has been used to study the effects of salt and protein loading on alpha-lactalbumin (BLA) stability during hydrophobic interaction chromatography (HIC)...
  28. Spencer W, Binette A, Ward T, Davis L, Blais D, Harrold J, et al. Alpha-lactalbumin in human milk alters the proteolytic degradation of soluble CD14 by forming a complex. Pediatr Res. 2010;68:490-3 pubmed publisher
    ..Mass spectrometry analysis of the protein identified alpha-lactalbumin. CD14 was also identified by immunoblot after immunoprecipitation of alpha-lactalbumin from milk...
  29. Mossberg A, Puchades M, Halskau Ø, Baumann A, Lanekoff I, Chao Y, et al. HAMLET interacts with lipid membranes and perturbs their structure and integrity. PLoS ONE. 2010;5:e9384 pubmed publisher
    ..HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a tumoricidal complex of partially unfolded alpha-lactalbumin (HLA) and oleic ..
  30. Wirmer J, Berk H, Ugolini R, Redfield C, Schwalbe H. Characterization of the unfolded state of bovine alpha-lactalbumin and comparison with unfolded states of homologous proteins. Protein Sci. 2006;15:1397-407 pubmed
    ..have been analyzed in the reduced unfolded states of hen lysozyme and the calcium-binding proteins bovine alpha-lactalbumin and human alpha-lactalbumin...
  31. Knyazeva E, Grishchenko V, Fadeev R, Akatov V, Permyakov S, Permyakov E. Who is Mr. HAMLET? Interaction of human alpha-lactalbumin with monomeric oleic acid. Biochemistry. 2008;47:13127-37 pubmed publisher
    A specific state of the human milk Ca(2+) binding protein alpha-lactalbumin (hLA) complexed with oleic acid (OA) prepared using an OA-pretreated ion-exchange column (HAMLET) triggers several cell death pathways in various tumor cells...
  32. Bushmarina N, Blanchet C, Vernier G, Forge V. Cofactor effects on the protein folding reaction: acceleration of alpha-lactalbumin refolding by metal ions. Protein Sci. 2006;15:659-71 pubmed
    ..The effects of cofactors on the folding reaction have been investigated with alpha-lactalbumin as a model protein and metal ions as cofactors...
  33. Pettersson J, Mossberg A, Svanborg C. alpha-Lactalbumin species variation, HAMLET formation, and tumor cell death. Biochem Biophys Res Commun. 2006;345:260-70 pubmed
    HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a tumoricidal complex of apo alpha-lactalbumin and oleic acid, formed in casein after low pH treatment of human milk...
  34. Zhang M, Yang F, Chen J, Zheng C, Liang Y. Cytotoxic aggregates of alpha-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells. Chem Biol Interact. 2009;180:131-42 pubmed publisher
    The effects of three fatty acids on cytotoxic aggregate formation of Ca(2+)-depleted bovine alpha-lactalbumin (apo-BLA) have been studied by UV absorbance spectroscopy and transmission electron microscopy...
  35. Ushida Y, Shimokawa Y, Toida T, Matsui H, Takase M. Bovine alpha-lactalbumin stimulates mucus metabolism in gastric mucosa. J Dairy Sci. 2007;90:541-6 pubmed
    Bovine alpha-lactalbumin (alpha-LA), a major milk protein, exerts strong gastroprotective activity against rat experimental gastric ulcers induced by ethanol or stress...
  36. Rammer P, Groth Pedersen L, Kirkegaard T, Daugaard M, Rytter A, Szyniarowski P, et al. BAMLET activates a lysosomal cell death program in cancer cells. Mol Cancer Ther. 2010;9:24-32 pubmed publisher
    A complex of human alpha-lactalbumin and oleic acid (HAMLET) was originally isolated from human milk as a potent anticancer agent...
  37. Storm P, Aits S, Puthia M, Urbano A, Northen T, Powers S, et al. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene. 2011;30:4765-79 pubmed publisher
    ..The results identify HAMLET as a novel anti-cancer agent that kills tumor cells by exploiting unifying features of cancer cells such as oncogene addiction or the Warburg effect. ..
  38. Singh S, Kishore N. Elucidating the binding thermodynamics of 8-anilino-1-naphthalene sulfonic acid with the A-state of alpha-lactalbumin: an isothermal titration calorimetric investigation. Biopolymers. 2006;83:205-12 pubmed
    ..of 8-anilino-1-naphthalene sulfonic acid (ANS) with the acid induced molten globule state (A-state) of alpha-lactalbumin (alpha-LA) is different from that with the native and denatured states of the protein...
  39. Mok K, Pettersson J, Orrenius S, Svanborg C. HAMLET, protein folding, and tumor cell death. Biochem Biophys Res Commun. 2007;354:1-7 pubmed
  40. Bumagina Z, Gurvits B, Artemova N, Muranov K, Yudin I, Kurganov B. Mechanism of suppression of dithiothreitol-induced aggregation of bovine alpha-lactalbumin by alpha-crystallin. Biophys Chem. 2010;146:108-17 pubmed publisher
    The kinetics of dithiothreitol (DTT)-induced aggregation of alpha-lactalbumin from bovine milk has been studied using dynamic light-scattering technique...
  41. Pettersson Kastberg J, Aits S, Gustafsson L, Mossberg A, Storm P, Trulsson M, et al. Can misfolded proteins be beneficial? The HAMLET case. Ann Med. 2009;41:162-76 pubmed publisher
    ..Here we present HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) as an example where partial unfolding and the incorporation of cofactor create a ..
  42. Ecroyd H, Meehan S, Horwitz J, Aquilina J, Benesch J, Robinson C, et al. Mimicking phosphorylation of alphaB-crystallin affects its chaperone activity. Biochem J. 2007;401:129-41 pubmed
  43. Baldassarre H, Schirm M, Deslauriers J, Turcotte C, Bordignon V. Protein profile and alpha-lactalbumin concentration in the milk of standard and transgenic goats expressing recombinant human butyrylcholinesterase. Transgenic Res. 2009;18:621-32 pubmed publisher
    ..between the transgenic and control groups included immunoglobulins, serum albumin, beta-lactoglobulin and alpha-lactalbumin. These results were found to be indicative of compromised epithelial tight junctions, premature mammary cell ..
  44. Correia M, Neves Petersen M, Parracino A, di Gennaro A, Petersen S. Photophysics, photochemistry and energetics of UV light induced disulphide bridge disruption in apo-?-lactalbumin. J Fluoresc. 2012;22:323-37 pubmed publisher
    Continuous 295 nm excitation of whey protein bovine apo-?-lactalbumin (apo-bLA) results in an increase of tryptophan fluorescence emission intensity, in a progressive red-shift of tryptophan fluorescence emission, and breakage of ..
  45. Hursel R, van der Zee L, Westerterp Plantenga M. Effects of a breakfast yoghurt, with additional total whey protein or caseinomacropeptide-depleted alpha-lactalbumin-enriched whey protein, on diet-induced thermogenesis and appetite suppression. Br J Nutr. 2010;103:775-80 pubmed publisher
    ..protein, on energy expenditure and satiety, yet a possible distinction between the effects of whey or alpha-lactalbumin has not been made...
  46. Trulsson M, Yu H, Gisselsson L, Chao Y, Urbano A, Aits S, et al. HAMLET binding to ?-actinin facilitates tumor cell detachment. PLoS ONE. 2011;6:e17179 pubmed publisher
    ..HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is a protein-lipid complex with tumoricidal activity that also triggers tumor cell ..
  47. Nakamura T, Makabe K, Tomoyori K, Maki K, Mukaiyama A, Kuwajima K. Different folding pathways taken by highly homologous proteins, goat alpha-lactalbumin and canine milk lysozyme. J Mol Biol. 2010;396:1361-78 pubmed publisher
    ..conserved in homologous proteins? To address this question, we compared the folding pathways of goat alpha-lactalbumin and canine milk lysozyme using equilibrium and kinetic circular dichroism spectroscopy...
  48. Spolaore B, Pinato O, Canton M, Zambonin M, Polverino De Laureto P, Fontana A. ?-Lactalbumin forms with oleic acid a high molecular weight complex displaying cytotoxic activity. Biochemistry. 2010;49:8658-67 pubmed publisher
    b>Lactalbumin (LA) forms with oleic acid (OA) a complex which has been reported to induce the selective death of tumor cells. However, the mechanism by which this complex kills a wide range of tumor cell lines is as yet largely unknown...
  49. Wang J, Yang P, Tang B, Sun X, Zhang R, Guo C, et al. Expression and characterization of bioactive recombinant human alpha-lactalbumin in the milk of transgenic cloned cows. J Dairy Sci. 2008;91:4466-76 pubmed publisher
    Improvement of the nutritional value of cow milk with transgenic expression of recombinant human alpha-lactalbumin (alpha-LA) has been previously attempted...
  50. Jaini R, Kesaraju P, Johnson J, Altuntas C, Jane wit D, Tuohy V. An autoimmune-mediated strategy for prophylactic breast cancer vaccination. Nat Med. 2010;16:799-803 pubmed publisher
    ..We selected alpha-lactalbumin as our target vaccine autoantigen because it is a breast-specific differentiation protein expressed in high ..
  51. Scrutton H, Carbonnier A, Cowen P, Harmer C. Effects of alpha-lactalbumin on emotional processing in healthy women. J Psychopharmacol. 2007;21:519-24 pubmed
    ..Recent studies suggest that alpha-lactalbumin, a whey-derived protein with a relatively high TRP content, increases plasma TRP and produces endocrine and ..
  52. Permyakov S, Khokhlova T, Uversky V, Permyakov E. Analysis of Ca2+/Mg2+ selectivity in alpha-lactalbumin and Ca(2+)-binding lysozyme reveals a distinct Mg(2+)-specific site in lysozyme. Proteins. 2010;78:2609-24 pubmed publisher
    ..Two homologous milk Ca(2+)-binding proteins, bovine alpha-lactalbumin (bLA) and equine lysozyme (EQL), were analyzed using the simplest "four-state" scheme of metal- and ..
  53. Mossberg A, Hou Y, Svensson M, Holmqvist B, Svanborg C. HAMLET treatment delays bladder cancer development. J Urol. 2010;183:1590-7 pubmed publisher
    ..Treatment groups received repeat intravesical HAMLET instillations and controls received alpha-lactalbumin or phosphate buffer...