internal ribosome entry sites


Summary: Sequences within MESSENGER RNA that enable PROTEIN TRANSLATION INITIATION independent of 5' CAPPED RNA.

Top Publications

  1. Dave P, George B, Sharma D, Das S. Polypyrimidine tract-binding protein (PTB) and PTB-associated splicing factor in CVB3 infection: an ITAF for an ITAF. Nucleic Acids Res. 2017;45:9068-9084 pubmed publisher
  2. Bugaud O, Barbier N, Chommy H, Fiszman N, Le Gall A, Dulin D, et al. Kinetics of CrPV and HCV IRES-mediated eukaryotic translation using single-molecule fluorescence microscopy. RNA. 2017;23:1626-1635 pubmed publisher
    ..Here, we use a noncanonical translation initiation based on internal ribosome entry sites (IRES), and we monitor the passage of individual, unmodified mammalian ribosomes at specific ..
  3. Sogorin E, Selikhanov G, Agalarov S. Coupling of Translation Initiation and Termination Does Not Depend on the Mode of Initiation. Biochemistry (Mosc). 2017;82:816-820 pubmed publisher
    ..Thus, the effect persists in the absence of scanning and does not depend on initiator tRNA and eIF2. The results suggest that the initiation factors are not involved in the coupling mechanism. ..
  4. Díaz Toledano R, Lozano G, Martinez Salas E. In-cell SHAPE uncovers dynamic interactions between the untranslated regions of the foot-and-mouth disease virus RNA. Nucleic Acids Res. 2017;45:1416-1432 pubmed publisher
  5. Tsetsarkin K, Liu G, Volkova E, Pletnev A. Synergistic Internal Ribosome Entry Site/MicroRNA-Based Approach for Flavivirus Attenuation and Live Vaccine Development. MBio. 2017;8: pubmed publisher
    ..This developed approach provides a robust platform for reliable attenuation of many flaviviruses and may enable development of live flavivirus vaccines. ..
  6. Deforges J, de Breyne S, Ameur M, Ulryck N, Chamond N, Saaidi A, et al. Two ribosome recruitment sites direct multiple translation events within HIV1 Gag open reading frame. Nucleic Acids Res. 2017;45:7382-7400 pubmed publisher
    ..It allows us to propose original hypotheses about the IRES physiological role and conservation among primate lentiviruses. ..
  7. Ee X, Yan Y, Hunter D, Schellhardt L, Sakiyama Elbert S, Mackinnon S, et al. Transgenic SCs expressing GDNF-IRES-DsRed impair nerve regeneration within acellular nerve allografts. Biotechnol Bioeng. 2017;114:2121-2130 pubmed publisher
    ..These data suggest a component of axon entrapment is independent of GDNF's impact on axons. Biotechnol. Bioeng. 2017;114: 2121-2130. © 2017 Wiley Periodicals, Inc. ..
  8. Ghassemi F, Madadgar O, Roohvand F, Rasekhian M, Etemadzadeh M, Boroujeni G, et al. [Translational efficiency of BVDV IRES and EMCV IRES for T7 RNA polymerase driven cytoplasmic expression in mammalian cell lines]. Mol Biol (Mosk). 2017;51:324-333 pubmed publisher
    ..Taken together, our results indicate the potential of BVDV IRES as a replacement for EMCV IRES, which is now commonly used for T7 polymerase driven cytoplasmic expression of genes of interest or virus cDNA rescue experiments. ..
  9. Yamamoto H, Unbehaun A, Spahn C. Ribosomal Chamber Music: Toward an Understanding of IRES Mechanisms. Trends Biochem Sci. 2017;42:655-668 pubmed publisher
    ..Because IRESs operate with reduced complexity and constitute minimal systems of initiation, comparison with canonical initiation may allow common mechanistic principles of the ribosome to be delineated. ..

More Information


  1. Ebadat S, Ahmadi S, Ahmadi M, Nematpour F, Barkhordari F, Mahdian R, et al. Evaluating the efficiency of CHEF and CMV promoter with IRES and Furin/2A linker sequences for monoclonal antibody expression in CHO cells. PLoS ONE. 2017;12:e0185967 pubmed publisher
    ..Our findings indicated that the CHEF promoter is a viable alternative to CMV promoter-based expression in F2A-mediated vectors by providing both higher expression and level of stability. ..
  2. Ismail R, Ul Hussain M. The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region. Biochimie. 2017;139:38-45 pubmed publisher
    ..Our data provides an interesting mechanism that may explain the increased glycolytic capacity of the astrocytes after brain hypoxia. ..
  3. Shi Z, Fujii K, Kovary K, Genuth N, Röst H, Teruel M, et al. Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide. Mol Cell. 2017;67:71-83.e7 pubmed publisher
    ..Within several of these transcripts, this level of regulation is mediated, at least in part, by internal ribosome entry sites. Together, these results reveal a critical functional link between ribosome heterogeneity and the post-..
  4. Gross L, Vicens Q, Einhorn E, Noireterre A, Schaeffer L, Kuhn L, et al. The IRES5'UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure. Nucleic Acids Res. 2017;45:8993-9004 pubmed publisher
    ..The IRES5'UTR contains a pseudoknot structure that is essential for proper folding and ribosome recruitment. Overall, our results pave the way for studies addressing the synergy and interplay between the two IRES from CrPV. ..
  5. Gritsenko A, Weingarten Gabbay S, Elias Kirma S, Nir R, de Ridder D, Segal E. Sequence features of viral and human Internal Ribosome Entry Sites predictive of their activity. PLoS Comput Biol. 2017;13:e1005734 pubmed publisher
    Translation of mRNAs through Internal Ribosome Entry Sites (IRESs) has emerged as a prominent mechanism of cellular and viral initiation...
  6. Galan A, Lozano G, Piñeiro D, Martinez Salas E. G3BP1 interacts directly with the FMDV IRES and negatively regulates translation. FEBS J. 2017;284:3202-3217 pubmed publisher
    ..Our results highlight the IRES-binding capacity of G3BP1 and illustrate its function as a translation inhibitor. ..