Summary: 1,N-6-Ethenoadenosine triphosphate. A fluorescent analog of adenosine triphosphate.
- Nowak E, Strzelecka Golaszewska H, Goody R. Kinetics of nucleotide and metal ion interaction with G-actin. Biochemistry. 1988;27:1785-92 pubmed..abstract truncated at 250 words) ..
- Todorov L, Mihaylova Todorova S, Westfall T, Sneddon P, Kennedy C, Bjur R, et al. Neuronal release of soluble nucleotidases and their role in neurotransmitter inactivation. Nature. 1997;387:76-9 pubmed..This release of specific nucleotidases together with ATP represents a new mechanism for terminating the actions of a neurotransmitter. ..
- Korenbaum E, Nordberg P, Björkegren Sjögren C, Schutt C, Lindberg U, Karlsson R. The role of profilin in actin polymerization and nucleotide exchange. Biochemistry. 1998;37:9274-83 pubmed
- Schobert B. Enzymatic synthesis of ATP analogs and their purification by reverse-phase high-performance liquid chromatography. Anal Biochem. 1995;226:288-92 pubmed..After freeze-drying of the pooled fractions, the yield of the synthesized nucleoside triphosphate was approximately 70%. The described procedures are applicable either for analytical investigations or for semi-preparative purposes. ..
- Diniz C, Fresco P, Goncalves J. Regional differences in extracellular purine degradation in the prostatic and epididymal portions of the rat vas deferens. Clin Exp Pharmacol Physiol. 2005;32:721-7 pubmed
- Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E. Severing of F-actin by yeast cofilin is pH-independent. Cell Motil Cytoskeleton. 2006;63:533-42 pubmed..The pH-independent severing by cofilin was confirmed using actin labeled at Cys374 with Oregon Green 488 maleimide. The depolymerization of actin by cofilin was faster at high pH. ..
- Ohm T, Wegner A. Random copolymerization of ATP-actin and ADP-actin. Biochemistry. 1991;30:11193-7 pubmed..According to the analysis of the critical concentrations, the equilibrium constants for incorporation of ATP-actin or ADP-actin into filaments were independent of the type of nucleotide bound to contiguous subunits. ..
- Lv X, Huang L, Chen W, Wang X, Huang Y, Deng C, et al. Molecular characterization and serological reactivity of a vacuolar ATP synthase subunit ?-like protein from Clonorchis sinensis. Parasitol Res. 2014;113:1545-54 pubmed publisher..This fundamental study would contribute to further researches that are related to growth and development and immunomodulation of C. sinensis. ..
- Pate E, Franks Skiba K, White H, Cooke R. The use of differing nucleotides to investigate cross-bridge kinetics. J Biol Chem. 1993;268:10046-53 pubmed..D., Belknap, B., and Jiang, W. (1993) J. Biol. Chem. 268, 10039-10045) is used to better define the actomyosin interaction in fibers. ..
- Gualix J, Alvarez A, Pintor J, Miras Portugal M. Studies of chromaffin granule functioning by flow cytometry: transport of fluorescent epsilon-ATP and granular size increase induced by ATP. Receptors Channels. 1999;6:449-61 pubmed..Moreover, no increase occurred in the presence of F- or acetate. The Cl- channel blockers were poorly effective, and only 2-(phenylamino)-benzoic acid (DPC) exhibited an effect on the ATP-induced granular size increase. ..
- Ooi A, Mihashi K. Effects of subtilisin cleavage of monomeric actin on its nucleotide binding. J Biochem. 1996;120:1104-10 pubmed..Furthermore, the kinetic analysis of ATP exchange revealed that the binding equilibrium between ATP and divalent cation-free cleaved G-actin was much slower than that in the case of intact G-actin. ..
- Fenton R, Dobson J. Fluorometric quantitation of adenosine concentration in small samples of extracellular fluid. Anal Biochem. 1992;207:134-41 pubmed..Thus, this assay is useful for determining the adenosine concentration in microliter samples of extracellular fluid and should facilitate investigations dealing with the functions of adenosine. ..
- Goldschmidt Clermont P, Machesky L, Doberstein S, Pollard T. Mechanism of the interaction of human platelet profilin with actin. J Cell Biol. 1991;113:1081-9 pubmed..Lindberg (1985. Nature [Lond.] 318:472-474) that polyphosphoinositides inhibit the effects of profilin on actin polymerization, so lipid metabolism must also be taken into account when considering the functions of profilin in a cell. ..
- Janmey P, Hvidt S, Oster G, Lamb J, Stossel T, Hartwig J. Effect of ATP on actin filament stiffness. Nature. 1990;347:95-9 pubmed..These data support earlier proposals that actin is not merely a passive cable, but has an active mechanochemical role in cell function. ..
- Obsil T, Merola F, Lewit Bentley A, Amler E. The isolated H4-H5 cytoplasmic loop of Na,K-ATPase overexpressed in Escherichia coli retains its ability to bind ATP. FEBS Lett. 1998;426:297-300 pubmed..Isolation of a soluble H4-H5 loop with the native ATP binding site is a crucial step for detailed studies of the molecular mechanism of ATP binding and utilisation. ..
- Franks Skiba K, Hwang T, Cooke R. Quenching of fluorescent nucleotides bound to myosin: a probe of the active-site conformation. Biochemistry. 1994;33:12720-8 pubmed..Thus nucleotides in the myosin pocket do not become more accessible to the solvent when myosin binds to actin in either rigor-ADP or active complexes.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Eads J, Mahoney N, Vorobiev S, Bresnick A, Wen K, Rubenstein P, et al. Structure determination and characterization of Saccharomyces cerevisiae profilin. Biochemistry. 1998;37:11171-81 pubmed..The in vivo and in vitro properties of yeast profilin mutants with altered poly-L-proline and actin binding sites are discussed in the context of the crystal structure. ..
- Virta P, Holmström T, Munter T, Nyholm T, Kronberg L, Sjöholm R. Fluorescent 7- and 8-methyl etheno derivatives of adenosine and 6-amino-9-ethylpurine: syntheses and fluorescence properties. Nucleosides Nucleotides Nucleic Acids. 2003;22:85-98 pubmed..Also, the stabilites of 5 and 7 in aqueous solutions were determined and found to be higher than that of the etheno derivative of adenosine. ..
- Sharon E, Zündorf G, Lévesque S, Beaudoin A, Reiser G, Fischer B. Fluorescent epsilon-ATP analogues for probing physicochemical properties of proteins. Synthesis, biochemical evaluation, and sensitivity to properties of the medium. Bioorg Med Chem. 2004;12:6119-35 pubmed..An inverse relationship and a linear relationship were found between the pK(a) values of 3b and the medium dielectricity and viscosity, respectively. These correlations help the calibration of properties of a protein ATP-binding site. ..
- Mayernik J, Conner M, Giam C. Methods for the isolation and detection of etheno adducts in nucleotide pools in vivo following exposure to ethyl carbamate. J Chromatogr. 1990;526:407-22 pubmed..Several etheno adducts (i.e. ethenoadenine, etheno-AMP, etheno-ADP and etheno-ATP) were also detected in total spleen cell nucleotide pools of trout following acute ethyl carbamate exposure. ..
- Miki M, Kouyama T. Domain motion in actin observed by fluorescence resonance energy transfer. Biochemistry. 1994;33:10171-7 pubmed..In G-actin, the mean distance between probes was 2.79 nm with a full width at half-maximum of 3.91 nm, indicating a large number of conformational substates in solution.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Conner M, Modzelewski R, Kawatani N. Sister chromatid exchange induced by etheno-ATP derivatives in vitro. Cancer Res. 1989;49:3839-43 pubmed
- Gualix J, Pintor J, Miras Portugal M. Characterization of nucleotide transport into rat brain synaptic vesicles. J Neurochem. 1999;73:1098-104 pubmed..The mitochondrial ATP/ADP exchange inhibitor atractyloside, N-ethylmaleimide, and polysulfonic aromatic compounds such as Evans blue and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid also inhibit epsilon-ATP vesicular transport. ..
- White H, Belknap B, Jiang W. Kinetics of binding and hydrolysis of a series of nucleoside triphosphates by actomyosin-S1. Relationship between solution rate constants and properties of muscle fibers. J Biol Chem. 1993;268:10039-45 pubmed..1993) to determine the average distance over which cross-bridges remain attached during unloaded shortening to be 5-12 nm. ..
- Kasprzak A. Myosin subfragment 1 activates ATP hydrolysis on Mg(2+)-G-actin. Biochemistry. 1994;33:12456-62 pubmed..The effect of deoxyribonuclease I on the rates of nucleotide dissociation and hydrolysis was examined.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Kim D, Churchich J. Active site modification of 4-aminobutyrate aminotransferase with ATP analogs. Biochim Biophys Acta. 1987;916:265-70 pubmed..It is postulated that the catalytic domain of 4-aminobutyrate aminotransferase is accessible to bulky reagents of greater length than the substrates 4-aminobutyrate and alpha-ketoglutarate. ..
- Frieden C, Patane K. Mechanism for nucleotide exchange in monomeric actin. Biochemistry. 1988;27:3812-20 pubmed..muscle G-actin has been treated to obtain ADP, 1,N6-ethenoadenosine diphosphate (epsilon-ADP), or 1,N6-ethenoadenosine triphosphate (epsilon-ATP) at the nucleotide binding site and either Mg2+ or Ca2+ at high- and moderate-affinity ..
- Xie L, Li W, Rhodes T, White H, Schoenberg M. Transient kinetic analysis of N-phenylmaleimide-reacted myosin subfragment-1. Biochemistry. 1999;38:5925-31 pubmed..W. (1978) Biochemistry 17, 3432]. However, NPM-modified S1 exhibited virtually no fluorescence enhancement upon ATP binding. This provides further evidence that M.ATP is the predominant intermediate of NPM-S1-catalyzed ATP hydrolysis. ..
- Eldin P, Le Cunff M, Vosberg H, Mornet D, Leger J. Mapping of the actomyosin interfaces. Proc Natl Acad Sci U S A. 1994;91:2772-6 pubmed..Rypnieski, R. W., Schmidt-Bäse, K., Smith, R., Tomchick, D. R., Benning, M. M., Winkelmann, D. A., Wesenberg, G. & Holden, H. M. (1993) Science 261, 50-58]. ..
- Kasprzak A. Myosin subfragment 1 inhibits dissociation of nucleotide and calcium from G-actin. J Biol Chem. 1993;268:13261-6 pubmed..The removal of the last 3 amino acids from actin produced a derivative whose behavior in binding to S1, as well as in the kinetics of epsilon ATP and Ca2+ dissociation, was undistinguishable from the unmodified protein. ..
- De La Cruz E, Pollard T. Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics. Biochemistry. 1995;34:5452-61 pubmed..2 x 10(-9) M for Mg(2+)-ATP-actin, 4.4 x 10(-9) M for Mg(2+)-epsilon ATP-actin, and 1.2 x 10(-10) M for Ca(2+)-ATP-actin. ..
- Gualix J, Abal M, Pintor J, Miras Portugal M. Presence of epsilon-adenosine tetraphosphate in chromaffin granules after transport of epsilon-ATP. FEBS Lett. 1996;391:195-8 pubmed..15 pmol/mg of granular protein). Intragranular concentrations of epsilon-ATP higher than 500 pmol/mg of protein (approximately to 175 microM intragranular) resulted in a non-saturable production of epsilon-Ap4. ..
- Root D, Reisler E. The accessibility of etheno-nucleotides to collisional quenchers and the nucleotide cleft in G- and F-actin. Protein Sci. 1992;1:1014-22 pubmed..Thus, the binding of S-1 induces conformational changes in the cleft region of actin that are different from those caused by Mg2+ polymerization of actin.(ABSTRACT TRUNCATED AT 250 WORDS) ..