adenylyl imidodiphosphate


Summary: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.

Top Publications

  1. Huang T, Suhan J, Hackney D. Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli. J Biol Chem. 1994;269:16502-7 pubmed
    ..Electron microscopy indicates that both DKH340 monomers and DKH392 dimers decorate microtubules with a periodicity of 8 nm. ..
  2. Vugmeyster Y, Berliner E, Gelles J. Release of isolated single kinesin molecules from microtubules. Biochemistry. 1998;37:747-57 pubmed
    ..The inhibitor adenylyl imidodiphosphate (AMP-PNP) induces stochastic pauses in the movement of bead-labeled enzyme molecules in 1 mM ATP...
  3. Powe A, Al Nakkash L, Li M, Hwang T. Mutation of Walker-A lysine 464 in cystic fibrosis transmembrane conductance regulator reveals functional interaction between its nucleotide-binding domains. J Physiol. 2002;539:333-46 pubmed
    ..Delay of closing in wild type by adenylyl imidodiphosphate (AMP-PNP), a non-hydrolysable ATP analogue, is markedly diminished in K464A mutants due to reduction in ..
  4. Skouboe C, Bjergbaek L, Oestergaard V, Larsen M, Knudsen B, Andersen A. A human topoisomerase II alpha heterodimer with only one ATP binding site can go through successive catalytic cycles. J Biol Chem. 2003;278:5768-74 pubmed
    ..The results can be explained in a model, where efficient catalysis requires an extensive communication between the second ATP and the DNA segment to be cleaved. ..
  5. Lamers M, Winterwerp H, Sixma T. The alternating ATPase domains of MutS control DNA mismatch repair. EMBO J. 2003;22:746-56 pubmed
    ..We propose that asymmetry of the ATPase domains is an essential feature of mismatch repair that controls the timing of the different steps in the repair cascade. ..
  6. Bjornson K, Modrich P. Differential and simultaneous adenosine di- and triphosphate binding by MutS. J Biol Chem. 2003;278:18557-62 pubmed
    ..These findings suggest that the protein exists primarily as the ATP.MutS.ADP ternary complex in solution and that this may be the form of the protein that is involved in DNA encounters in vivo. ..
  7. Wigley D, Davies G, Dodson E, Maxwell A, Dodson G. Crystal structure of an N-terminal fragment of the DNA gyrase B protein. Nature. 1991;351:624-9 pubmed
    ..The C-terminal domains form the sides of a 20 A hole through the protein dimer which may play a role in DNA strand passage during the supercoiling reaction. ..
  8. Hubbard S. Crystal structure of the activated insulin receptor tyrosine kinase in complex with peptide substrate and ATP analog. EMBO J. 1997;16:5572-81 pubmed
    ..The structure thus reveals the molecular basis for insulin receptor activation via autophosphorylation, and provides insights into tyrosine kinase substrate specificity and the mechanism of phosphotransfer. ..
  9. Kawaguchi K, Ishiwata S. Nucleotide-dependent single- to double-headed binding of kinesin. Science. 2001;291:667-9 pubmed
    ..Thus, kinesin binds through two heads in the former and one head in the latter two states, which supports a major prediction of the hand-over-hand model. ..

More Information

Publications110 found, 100 shown here

  1. Aleksandrov L, Mengos A, Chang X, Aleksandrov A, Riordan J. Differential interactions of nucleotides at the two nucleotide binding domains of the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 2001;276:12918-23 pubmed
    ..This argues strongly that AMP-PNP does not inhibit ATP hydrolysis at NBD2 and thereby questions the relevance of hydrolysis at that domain to channel closing. ..
  2. Wei H, Ruthenburg A, Bechis S, Verdine G. Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase. J Biol Chem. 2005;280:37041-7 pubmed
    ..Comparison of these structures revealed rigid-body movement of the structural modules within the ATPase domain, suggestive of the motions of a molecular gate. ..
  3. Dawson R, Locher K. Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP. FEBS Lett. 2007;581:935-8 pubmed
    ..The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains. ..
  4. Ward A, Reyes C, Yu J, Roth C, Chang G. Flexibility in the ABC transporter MsbA: Alternating access with a twist. Proc Natl Acad Sci U S A. 2007;104:19005-10 pubmed
    ..Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport...
  5. Medvedev A, Sandler M, Glover V. The influence of isatin on guanylyl cyclase of rat heart membranes. Eur J Pharmacol. 1999;384:239-41 pubmed
    ..These results indicate that isatin may interact with GC independently of its regulation by natriuretic peptides. ..
  6. Krishna S, Zhou T, Daugherty M, Osterman A, Zhang H. Structural basis for the catalysis and substrate specificity of homoserine kinase. Biochemistry. 2001;40:10810-8 pubmed
    ..Therefore, we propose that the catalytic mechanism of HSK does not involve a catalytic base for activating the phosphoryl acceptor hydroxyl but instead is mediated via a transition state stabilization mechanism...
  7. Urbach V, Hélix N, Renaudon B, Harvey B. Cellular mechanisms for apical ATP effects on intracellular pH in human bronchial epithelium. J Physiol. 2002;543:13-21 pubmed
    ..We interpret the effect of ATP to produce an intracellular acidification as a three step process: activation of H+ channels, inhibition of Na+-H+ exchange and influx of protonated ATP. ..
  8. Llorca O, Martin Benito J, Grantham J, Ritco Vonsovici M, Willison K, Carrascosa J, et al. The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin. EMBO J. 2001;20:4065-75 pubmed
    ..Docking of the AMP-PNP-CCT-bound conformations of alpha-actin and beta-tubulin to their respective native atomic structures suggests that both proteins have progressed towards their native states. ..
  9. Hou Y, Cui L, Riordan J, Chang X. ATP binding to the first nucleotide-binding domain of multidrug resistance protein MRP1 increases binding and hydrolysis of ATP and trapping of ADP at the second domain. J Biol Chem. 2002;277:5110-9 pubmed
    ..The interactions between the two asymmetric NBDs of MRP1 protein may enhance the catalytic efficiency of the MRP1 protein and hence of its ATP-dependent transport of conjugated anions out of cells. ..
  10. Qian K, Wang L, Hickey E, Studts J, Barringer K, Peng C, et al. Structural basis of constitutive activity and a unique nucleotide binding mode of human Pim-1 kinase. J Biol Chem. 2005;280:6130-7 pubmed
    ..Analysis of the reported Pim-1 kinase-domain structures leads to a hypothesis as to how Pim kinase activity might be regulated in vivo. ..
  11. Jovanovic M, Dynan W. Terminal DNA structure and ATP influence binding parameters of the DNA-dependent protein kinase at an early step prior to DNA synapsis. Nucleic Acids Res. 2006;34:1112-20 pubmed
    ..ATP and a nonhydrolyzable ATP analog also influence the stability of the DNA-PKcs*DNA complex, apparently by an allosteric mechanism that does not require DNA-PKcs autophosphorylation. ..
  12. Belous A, Jones C, Wakata A, Knox C, Nicoud I, Pierce J, et al. Mitochondrial calcium transport is regulated by P2Y1- and P2Y2-like mitochondrial receptors. J Cell Biochem. 2006;99:1165-74 pubmed
    ..These data strongly support the hypothesis that mitochondrial Ca(2+) uptake is regulated in part by adenine nucleotides via a P2Y-like receptor mechanism that involves mitochondrial PLC activation. ..
  13. Del Campo M, Lambowitz A. Crystallization and preliminary X-ray diffraction of the DEAD-box protein Mss116p complexed with an RNA oligonucleotide and AMP-PNP. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:832-5 pubmed publisher
    ..54, b = 126.52, c = 55.52 A, and diffracted X-rays to beyond 1.9 A resolution using synchrotron radiation from sector 21 at the Advanced Photon Source. ..
  14. Wang J, Steinbacher S, Augustin M, Schreiner P, Epstein D, Mulvihill M, et al. The crystal structure of a constitutively active mutant RON kinase suggests an intramolecular autophosphorylation hypothesis. Biochemistry. 2010;49:7972-4 pubmed publisher
    ..The structure shows how the oncogenic mutation may cause the constitutive activation and suggests a mechanistic hypothesis for the autophosphorylation of receptor tyrosine kinases. ..
  15. Byrnes L, Xu Y, Qiu X, Hall J, West G. Sites associated with Kalydeco binding on human Cystic Fibrosis Transmembrane Conductance Regulator revealed by Hydrogen/Deuterium Exchange. Sci Rep. 2018;8:4664 pubmed publisher
    ..The ligand-induced changes of CFTR in the presence of Kalydeco suggest a potential binding site. ..
  16. O Donnell M. Accessory proteins bind a primed template and mediate rapid cycling of DNA polymerase III holoenzyme from Escherichia coli. J Biol Chem. 1987;262:16558-65 pubmed
    ..These subunit dynamics suggest mechanisms for polymerase cycling on the lagging strand of replication forks in a growing chromosome. ..
  17. Foster K, Correia J, Gilbert S. Equilibrium binding studies of non-claret disjunctional protein (Ncd) reveal cooperative interactions between the motor domains. J Biol Chem. 1998;273:35307-18 pubmed
    ..MC1 complex. These results are consistent with a model for the ATPase cycle in which ATP hydrolysis occurs on the microtubule followed by detachment as the Ncd.ADP.Pi intermediate. ..
  18. Galletto R, Jezewska M, Bujalowski W. Interactions of the Escherichia coli DnaB helicase hexamer with the replication factor the DnaC protein. Effect of nucleotide cofactors and the ssDNA on protein-protein interactions and the topology of the complex. J Mol Biol. 2003;329:441-65 pubmed
    ..In solution, the DnaC protein-binding site is located on the large 33 kDa domain of the DnaB helicase. The significance of the results in the functioning of the DnaB helicase-DnaC protein complex is discussed. ..
  19. Subramanian R, Gelles J. Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP. J Gen Physiol. 2007;130:445-55 pubmed
    ..In one of these, the enzyme can accomplish high-duty-ratio processive movement without alternating-sites ATP hydrolysis. ..
  20. Tsai N, Tsui Y, Wei L. Dynein motor contributes to stress granule dynamics in primary neurons. Neuroscience. 2009;159:647-56 pubmed publisher
    ..These findings demonstrate, for the first time, that dynein motor complex plays a critical role in the dynamics of neuronal SGs, as well as translation of certain mRNAs. ..
  21. Yengo C, De La Cruz E, Safer D, Ostap E, Sweeney H. Kinetic characterization of the weak binding states of myosin V. Biochemistry. 2002;41:8508-17 pubmed
  22. Shorter J, Lindquist S. Destruction or potentiation of different prions catalyzed by similar Hsp104 remodeling activities. Mol Cell. 2006;23:425-38 pubmed
    ..In vivid distinction, the endpoint of Ure2 fragmentation is short prion fibers with enhanced infectivity and self-replicating ability. These advances explain the distinct effects of Hsp104 on the inheritance of the two prions. ..
  23. Papanikolau Y, Papadovasilaki M, Ravelli R, McCarthy A, Cusack S, Economou A, et al. Structure of dimeric SecA, the Escherichia coli preprotein translocase motor. J Mol Biol. 2007;366:1545-57 pubmed
    ..The repercussion of these findings on translocase assembly and catalysis is discussed. ..
  24. Parke C, Wojcik E, Kim S, Worthylake D. ATP hydrolysis in Eg5 kinesin involves a catalytic two-water mechanism. J Biol Chem. 2010;285:5859-67 pubmed publisher
    ..Coordination of switch movements via sequential proton transfer along paired water clusters may be universal for nucleotide triphosphatases with conserved active sites, such as myosins and G-proteins. ..
  25. Kundukad B, van der Maarel J. Control of the flow properties of DNA by topoisomerase II and its targeting inhibitor. Biophys J. 2010;99:1906-15 pubmed publisher
    ..A total replacement of ATP by AMP-PNP results in a temporal increase in elasticity at higher frequencies, but no transition to an elastic gel with fixed cross-links. ..
  26. Liu B, Zhang C, Qin F. Functional recovery from desensitization of vanilloid receptor TRPV1 requires resynthesis of phosphatidylinositol 4,5-bisphosphate. J Neurosci. 2005;25:4835-43 pubmed
  27. Moreira O, Rios P, Barrabin H. Inhibition of plasma membrane Ca(2+)-ATPase by CrATP. LaATP but not CrATP stabilizes the Ca(2+)-occluded state. Biochim Biophys Acta. 2005;1708:411-9 pubmed
    ..This intermediate is stabilized by LaATP but not by CrATP. ..
  28. Ward A, Mulligan S, Carragher B, Chang G, Milligan R. Nucleotide dependent packing differences in helical crystals of the ABC transporter MsbA. J Struct Biol. 2009;165:169-75 pubmed publisher
    ..This suggests that while there are likely structural differences between the nucleotide transition states, membrane embedded MsbA remains in an outward facing conformation while nucleotide is bound...
  29. Gardam K, Magoski N. Regulation of cation channel voltage and Ca2+ dependence by multiple modulators. J Neurophysiol. 2009;102:259-71 pubmed publisher
    ..This ensures hormone delivery by allowing afterdischarge initiation and maintenance but also prevents interminable bursting. Similar regulatory interactions may be used by other neurons to achieve diverse outputs. ..
  30. Schalk Hihi C, Markham G. The conformations of a substrate and a product bound to the active site of S-adenosylmethionine synthetase. Biochemistry. 1999;38:2542-50 pubmed
    ..nuclear Overhauser effect spectroscopy to determine the conformation of enzyme-bound AdoMet and 5'-adenylyl imidodiphosphate (AMPPNP)...
  31. Zhu Y, Huang W, Lee S, Xu W. Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis. EMBO Rep. 2005;6:681-7 pubmed
    ..The PPK structure has laid the foundation for understanding the initiation of polyP synthesis by PPK. ..
  32. Korkhov V, Mireku S, Locher K. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012;490:367-72 pubmed publisher
    ..In combination with engineered disulphide crosslinking and functional assays, our data suggest an unexpected peristaltic transport mechanism that is distinct from those observed in other ABC transporters. ..
  33. van Voorst F, Vereyken I, De Kruijff B. The high affinity ATP binding site modulates the SecA-precursor interaction. FEBS Lett. 2000;486:57-62 pubmed
    ..Examining SecA ATP binding site mutants demonstrated that ATP hydrolysis at the N-terminal high affinity binding site is responsible for the changed interaction with the preprotein. ..
  34. Wray J, Jahn W. Gamma-amido-ATP stabilizes a high-fluorescence state of myosin subfragment 1. FEBS Lett. 2002;518:97-100 pubmed
    ..ATPgammaNH2 appears to stabilize the 'closed' conformation of S1, and does so without cleavage of the beta-gamma phosphate bond. The dissociation of actin-S1 by ATPgammaNH2 and that of S1.ATPgammaNH2 by actin are both strikingly slow. ..
  35. Kukimoto Niino M, Tokmakov A, Terada T, Ohbayashi N, Fujimoto T, Gomi S, et al. Inhibitor-bound structures of human pyruvate dehydrogenase kinase 4. Acta Crystallogr D Biol Crystallogr. 2011;67:763-73 pubmed publisher
    ..Thus, the present structures demonstrate for the first time the flexible and dynamic aspects of PDK4 in the open conformation and provide a basis for the development of novel inhibitors targeting the nucleotide-binding pocket of PDK4. ..
  36. Kawai S, Cooper D, Landes C, Mootz H, Yang H, Komatsuzaki T. Numerical construction of estimators for single-molecule fluorescence measurements. J Phys Chem B. 2013;117:8061-74 pubmed publisher
    ..It is shown that the estimators constructed by the present method can quantify faithfully not only the physical quantity to be monitored but also the functions of that quantity for a wide range of values. ..
  37. Jain R, Khan N, Menzel A, Rajkovic I, Konrad M, Techert S. Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering. Eur Biophys J. 2016;45:81-9 pubmed publisher
    ..The structural changes between open and completely closed hGMPK conformation were confirmed by observing differences in the hGMPK secondary structures with circular dichroism spectroscopy. ..
  38. Kurihara K, Nakanishi N, Ueha T. A calcium channel in human submandibular duct cell line, HSG cells, not regulated by P2U purinergic receptor-mediated intracellular calcium mobilization. Arch Oral Biol. 1997;42:547-57 pubmed
    ..Thus, in HSG cells, IP3 formation is coupled to the P2U subclass, while 45Ca2+ influx is coupled to another subclass, such as P2X, that regulates calcium channels...
  39. Zhu X, Kim J, Newcomb J, Rose P, Stover D, Toledo L, et al. Structural analysis of the lymphocyte-specific kinase Lck in complex with non-selective and Src family selective kinase inhibitors. Structure. 1999;7:651-61 pubmed
    ..The structures of these Lck complexes offer useful structural insights as they demonstrate that kinase selectivity can be achieved with small-molecule inhibitors that exploit subtle topological differences among protein kinases. ..
  40. Hartvig N, Lorinczy D, Farkas N, Belagyi J. Effect of adenosine 5'-[beta,gamma-imido]triphosphate on myosin head domain movements. Eur J Biochem. 2002;269:2168-77 pubmed
    ..In the AdoPP[CH2]P state, approximately half of the myosin heads reflected the motional state of ADP-myosin, and the other half showed a different dynamic state with greater mobility. ..
  41. Sedletska Y, Fourrier L, Malinge J. Modulation of MutS ATP-dependent functional activities by DNA containing a cisplatin compound lesion (base damage and mismatch). J Mol Biol. 2007;369:27-40 pubmed
    ..We propose that DNA bending could play a role in MutS biochemical modulations induced by a compound lesion and that cisplatin DNA damage signaling by the MMR system could be modulated in a direct mode. ..
  42. Berger S, Evans P. Steady-state fluorescence of Escherichia coli phosphofructokinase reveals a regulatory role for ATP. Biochemistry. 1991;30:8477-80 pubmed
    ..coli PFK exists in a state with high affinity for Fru6P ("R" state). This state can be altered to a low affinity ("T" state) by PEP binding to the allosteric site or by ATP binding to the enzyme. ..
  43. Markham G, Satishchandran C. Identification of the reactive sulfhydryl groups of S-adenosylmethionine synthetase. J Biol Chem. 1988;263:8666-70 pubmed
    ..Upon modification the strength of dimer-dimer interactions is diminished by at least 9 kcal/mol. ..
  44. Huang H, Goldberg A. Proteolytic activity of the ATP-dependent protease HslVU can be uncoupled from ATP hydrolysis. J Biol Chem. 1997;272:21364-72 pubmed
    ..However, AMP-PNP binding together with K+ can induce a form of HslVU that degrades proteins without energy consumption. ..
  45. Schmitt T, Hotz Wagenblatt A, Klein H, Dröge W. Interdependent regulation of insulin receptor kinase activity by ADP and hydrogen peroxide. J Biol Chem. 2005;280:3795-801 pubmed
  46. Cheng Z, Muhlrad D, Lim M, Parker R, Song H. Structural and functional insights into the human Upf1 helicase core. EMBO J. 2007;26:253-64 pubmed
    ..These conformational changes alter the likely ssRNA-binding channel in a manner that can explain how ATP binding destabilizes ssRNA binding to Upf1p...
  47. Banerjee R, Jayaraj G, Peter J, Kumar V, Mapa K. Monitoring conformational heterogeneity of the lid of DnaK substrate-binding domain during its chaperone cycle. FEBS J. 2016;283:2853-68 pubmed publisher
    ..Lid closure is only partial upon binding to molten globule-like authentic cellular substrates, probably to accommodate non-native substrate proteins of varied structures. ..
  48. Robinson M, Osheroff N. Effects of antineoplastic drugs on the post-strand-passage DNA cleavage/religation equilibrium of topoisomerase II. Biochemistry. 1991;30:1807-13 pubmed
    ..These results strongly suggest that both the pre- and post-strand-passage DNA cleavage complexes of topoisomerase II serve as physiological targets for these structurally disparate antineoplastic drugs. ..
  49. Imai S, Chin W, Jin H, Nakazawa M. Production of AMP and adenosine in the interstitial fluid compartment of the isolated perfused normoxic guinea pig heart. Pflugers Arch. 1989;414:443-9 pubmed
    ..As AMPPNP and AMPPCP are good substrates of ATP pyrophosphohydrolase, these findings indicate the presence of ATP pyrophosphohydrolase in the myocardial interstitial space. ..
  50. van Raaij M, Abrahams J, Leslie A, Walker J. The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B. Proc Natl Acad Sci U S A. 1996;93:6913-7 pubmed
    ..Aurovertin B appears to act by preventing closure of the catalytic interfaces, which is essential for a catalytic mechanism involving cyclic interconversion of catalytic sites. ..
  51. Varga A, Szabo J, Flachner B, Gugolya Z, Vonderviszt F, Zavodszky P, et al. Thermodynamic analysis of substrate induced domain closure of 3-phosphoglycerate kinase. FEBS Lett. 2009;583:3660-4 pubmed publisher
    ..Our results suggest that conformational rearrangements in the hinge generated by binding of both substrates provide the main driving force for domain closure overcoming the slightly unfavourable contact interactions between the domains. ..
  52. Malakhova M, Kurinov I, Liu K, Zheng D, D Angelo I, Shim J, et al. Structural diversity of the active N-terminal kinase domain of p90 ribosomal S6 kinase 2. PLoS ONE. 2009;4:e8044 pubmed publisher
    ..The presence of a unique betaB-sheet insert in the N-lobe suggests a different type of activation mechanism for RSK2. ..
  53. Menz R, Leslie A, Walker J. The structure and nucleotide occupancy of bovine mitochondrial F(1)-ATPase are not influenced by crystallisation at high concentrations of nucleotide. FEBS Lett. 2001;494:11-4 pubmed
    ..Similar to previous structures of bovine F(1)-ATPase determined with crystals grown in the presence of lower nucleotide concentrations, only two beta-subunits have bound nucleotide and the third subunit remains empty. ..
  54. Kawamura Y, Arakawa K, Maeshima M, Yoshida S. ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPase. Eur J Biochem. 2001;268:2801-9 pubmed
    ..This is the first demonstration of interaction between the A and E subunits in the substrate-binding state of a plant V-ATPase. ..
  55. Hayashi F, Ito H, Fujita M, Iwase R, Uzumaki T, Ishiura M. Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC. Biochem Biophys Res Commun. 2004;316:195-202 pubmed
  56. Li J, Richter K, Buchner J. Mixed Hsp90-cochaperone complexes are important for the progression of the reaction cycle. Nat Struct Mol Biol. 2011;18:61-6 pubmed publisher
    ..This mechanism, which is strictly conserved between the yeast and human Hsp90 systems, presents an example of how, in a cyclic process, directionality of assembly and disassembly of protein complexes can be achieved. ..
  57. Jedrzejczak R, Wang J, Dauter M, Szczesny R, Stepien P, Dauter Z. Human Suv3 protein reveals unique features among SF2 helicases. Acta Crystallogr D Biol Crystallogr. 2011;67:988-96 pubmed publisher
    ..Most of the typical helicase motifs are present in hSuv3, but the protein shows certain unique characteristics, suggesting that Suv3 enzymes may constitute a separate subfamily of helicases. ..
  58. Gamas P, Craig N. Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA. Nucleic Acids Res. 1992;20:2525-32 pubmed
    ..We speculate that TnsC plays a central role in the selection of target DNA during Tn7 transposition. ..
  59. El Mezgueldi M, Marston S. The effects of smooth muscle calponin on the strong and weak myosin binding sites of F-actin. J Biol Chem. 1996;271:28161-7 pubmed
    ..MgAMP-PNP (5'-adenylyl imidodiphosphate) and on the weak binding of S-1.MgADP.Pi to actin...
  60. Gibbons D, Hixson J, Hay N, Lund P, Gorovits B, Ybarra J, et al. Intrinsic fluorescence studies of the chaperonin GroEL containing single Tyr --> Trp replacements reveal ligand-induced conformational changes. J Biol Chem. 1996;271:31989-95 pubmed
    ..These results are discussed in relation to the molecular mechanism for GroEL function. ..
  61. Hall M, Matson S. The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity. J Biol Chem. 1999;274:1306-12 pubmed
    ..These results suggest that MutL activates the MutH-associated endonuclease activity through a physical interaction during methyl-directed mismatch repair in Escherichia coli. ..
  62. Belous A, Knox C, Nicoud I, Pierce J, Anderson C, Pinson C, et al. Altered ATP-dependent mitochondrial Ca2+ uptake in cold ischemia is attenuated by ruthenium red. J Surg Res. 2003;111:284-9 pubmed
    ..These findings may offer a potentially effective strategy for prevention of ischemia-reperfusion injury in liver transplantation. ..
  63. Robien M, Krumm B, Sandkvist M, Hol W. Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae. J Mol Biol. 2003;333:657-74 pubmed
    ..The N2-domain-containing surface of the hexamer appears to be highly conserved in the GspE family and most likely faces the inner membrane interacting with other members of the Eps system...
  64. Volkmann N, Liu H, Hazelwood L, Krementsova E, Lowey S, Trybus K, et al. The structural basis of myosin V processive movement as revealed by electron cryomicroscopy. Mol Cell. 2005;19:595-605 pubmed
    ..Our findings reveal how the structural elements of myosin V work together to allow myosin V to step along actin for multiple ATPase cycles without dissociating. ..
  65. Kanzaki M, Lindorfer M, Garrison J, Kojima I. Activation of the calcium-permeable cation channel CD20 by alpha subunits of the Gi protein. J Biol Chem. 1997;272:14733-9 pubmed
    ..These findings indicate that the CD20 channel expressed in Balb/c 3T3 cells is activated by the IGF-I receptor via the alpha subunits of heterotrimeric G proteins. ..
  66. Kwok Y, Hurley L. Topoisomerase II site-directed alkylation of DNA by psorospermin and its effect on topoisomerase II-mediated DNA cleavage. J Biol Chem. 1998;273:33020-6 pubmed
  67. Lin Y, Nageswara Rao B. Structural characterization of adenine nucleotides bound to Escherichia coli adenylate kinase. 2. 31P and 13C relaxation measurements in the presence of cobalt(II) and manganese(II). Biochemistry. 2000;39:3647-55 pubmed
    ..These conformations differ significantly from the nucleotide conformations in crystals of AKe. AP(5)A and AKe.AMP.AMPPNP as determined by X-ray crystallography. ..
  68. Dzeja P, Bortolon R, Perez Terzic C, Holmuhamedov E, Terzic A. Energetic communication between mitochondria and nucleus directed by catalyzed phosphotransfer. Proc Natl Acad Sci U S A. 2002;99:10156-61 pubmed
    ..Thus, mitochondrial oxidative phosphorylation coupled with phosphotransfer relays provides an efficient energetic unit in support of nuclear transport. ..
  69. Alonso M, Drummond D, Kain S, Hoeng J, Amos L, Cross R. An ATP gate controls tubulin binding by the tethered head of kinesin-1. Science. 2007;316:120-3 pubmed
    ..The data reveal an ATP gate that operates independently of the microtubule lattice, by ATP-dependent release of a steric or allosteric block on the tubulin binding site of the tethered kinesin-ADP head. ..
  70. Lin L, Czerwinski R, Kelleher K, Siegel M, Wu P, Kriz R, et al. Activation loop phosphorylation modulates Bruton's tyrosine kinase (Btk) kinase domain activity. Biochemistry. 2009;48:2021-32 pubmed publisher
    ..Taken together, our study provides important mechanistic insights into Btk kinase activity and phosphorylation-mediated regulation. ..
  71. Ferguson A, Sheth P, Basso A, Paliwal S, Gray K, Fischmann T, et al. Structural basis of CX-4945 binding to human protein kinase CK2. FEBS Lett. 2011;585:104-10 pubmed publisher
    ..7 and 1.3 Å, respectively. Biophysical analysis of CX-4945 binding is also described. This data provides the structural rationale for the design of more potent inhibitors against this emerging cancer target. ..
  72. Auzat I, Gawlita E, Garel J. Slow ligand-induced transitions in the allosteric phosphofructokinase from Escherichia coli. J Mol Biol. 1995;249:478-92 pubmed
    ..These results show that steady-state measurements are not sufficient to analyze the regulatory properties of E. coli phosphofructokinase. ..
  73. Pintor J, Díaz Rey M, Miras Portugal M. Ap4A and ADP-beta-S binding to P2 purinoceptors present on rat brain synaptic terminals. Br J Pharmacol. 1993;108:1094-9 pubmed
    ..212 microM, 0.481 microM and 18.04 microM respectively.6. These studies suggest the presence of a new P2-purinoceptor in rat brain synaptosomes with high affinity for diadenosine polyphosphates which we tentatively designate as P2d. ..
  74. Hirose K, Lockhart A, Cross R, Amos L. Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules. Proc Natl Acad Sci U S A. 1996;93:9539-44 pubmed
    ..There is also a distinct difference in orientation: the second kinesin head is tilted toward the microtubule plus end, while the second head of ncd points toward the minus end. ..
  75. Velankar S, Soultanas P, Dillingham M, Subramanya H, Wigley D. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism. Cell. 1999;97:75-84 pubmed
    ..Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism. ..
  76. Inagaki E, Ukita Y, Kumei M, Kajihara Y, Tahirov T. Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 2004;60:761-3 pubmed
    ..72, c = 321.61 A and with bound KDG plus the ATP analogue AMP-PNP (TtKDGK-ATP-KDG), with unit-cell parameters a = b = 84.32, c = 168.7 A, were also prepared and characterized. ..
  77. Kapoor T, Mitchison T. Allele-specific activators and inhibitors for kinesin. Proc Natl Acad Sci U S A. 1999;96:9106-11 pubmed
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