Sindbis virus

Summary

Alias: Sindbis virus dsSIN

Top Publications

  1. Rice C, Strauss J. Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins. Proc Natl Acad Sci U S A. 1981;78:2062-6 pubmed
    The nucleotide sequence of intracellular 26S mRNA of Sindbis virus has been determined by direct sequence analysis of the cDNA made to this RNA with reverse transcriptase...
  2. Warrier R, Linger B, Golden B, Kuhn R. Role of sindbis virus capsid protein region II in nucleocapsid core assembly and encapsidation of genomic RNA. J Virol. 2008;82:4461-70 pubmed publisher
    b>Sindbis virus is an enveloped positive-sense RNA virus in the alphavirus genus. The nucleocapsid core contains the genomic RNA surrounded by 240 copies of a single capsid protein...
  3. Park E, Griffin D. Interaction of Sindbis virus non-structural protein 3 with poly(ADP-ribose) polymerase 1 in neuronal cells. J Gen Virol. 2009;90:2073-80 pubmed publisher
    ..domain can bind poly(ADP-ribose) (PAR), and PAR polymerase-1 (PARP-1) is activated and autoribosylated during Sindbis virus (SINV) infection, it was hypothesized that PARP-1 and nsP3 may interact...
  4. Strauss E, Rice C, Strauss J. Complete nucleotide sequence of the genomic RNA of Sindbis virus. Virology. 1984;133:92-110 pubmed
    The entire nucleotide sequence of the genomic RNA of the type virus of the alphavirus genus, Sindbis virus, has been determined. The genome is 11,703 nucleotides in length, exclusive of the 5' cap and the 3'-terminal poly(A) tract...
  5. Thal M, Wasik B, Posto J, Hardy R. Template requirements for recognition and copying by Sindbis virus RNA-dependent RNA polymerase. Virology. 2007;358:221-32 pubmed publisher
    The Sindbis virus (SIN) nonstructural protein nsP4 possesses the RNA-dependent RNA polymerase activity required for the replication of the SIN genome and transcription of a subgenomic mRNA during infection...
  6. Li M, Stollar V. Distinct sites on the Sindbis virus RNA-dependent RNA polymerase for binding to the promoters for the synthesis of genomic and subgenomic RNA. J Virol. 2007;81:4371-3 pubmed publisher
    b>Sindbis virus-infected cells make two positive-strand RNAs, a genomic (G) RNA and a subgenomic (SG) RNA...
  7. Geders T, Smith J, Kuhn R. Role for conserved residues of sindbis virus nonstructural protein 2 methyltransferase-like domain in regulation of minus-strand synthesis and development of cytopathic infection. J Virol. 2008;82:7284-97 pubmed publisher
    The plus-strand RNA genome of Sindbis virus (SINV) encodes four nonstructural proteins (nsP1 to nsP4) that are involved in the replication of the viral RNA...
  8. Zhu W, Fu S, Wang J, He Y, Tang Q, Liang G. Effects of the nsP2-726 Pro mutation on infectivity and pathogenesis of Sindbis virus derived from a full-length infectious cDNA clone. Virus Res. 2009;142:204-7 pubmed publisher
    The point mutations at residue 726 Pro in the nonstructural gene 2 (nsP2-726P) could make Sindbis virus (SINV) replicons lacking the structural protein-coding region less cytopathic and capable of persisting in some vertebrate cell lines...
  9. Frolov I, Garmashova N, Atasheva S, Frolova E. Random insertion mutagenesis of sindbis virus nonstructural protein 2 and selection of variants incapable of downregulating cellular transcription. J Virol. 2009;83:9031-44 pubmed publisher
    b>Sindbis virus nonstructural protein 2 (SINV nsP2) is an important determinant of virus pathogenesis and downregulation of virus-induced cell response...

More Information

Publications138 found, 100 shown here

  1. Zhu W, Wang L, Yang Y, Jia J, Fu S, Feng Y, et al. Interaction of E2 glycoprotein with heparan sulfate is crucial for cellular infection of Sindbis virus. PLoS ONE. 2010;5:e9656 pubmed publisher
    Cell culture-adapted strains of Sindbis virus (SINV) initially attach to cells by the ability to interact with heparan sulfate (HS) through selective mutation for positively charged amino acid (aa) scattered in E2 glycoprotein (W. B...
  2. Cristea I, Rozjabek H, Molloy K, Karki S, White L, Rice C, et al. Host factors associated with the Sindbis virus RNA-dependent RNA polymerase: role for G3BP1 and G3BP2 in virus replication. J Virol. 2010;84:6720-32 pubmed publisher
    b>Sindbis virus (SINV) is the prototype member of the Alphavirus genus, whose members cause severe human diseases for which there is no specific treatment...
  3. Tang J, Jose J, Chipman P, Zhang W, Kuhn R, Baker T. Molecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus. J Mol Biol. 2011;414:442-59 pubmed publisher
    A three-dimensional reconstruction of Sindbis virus at 7...
  4. Jose J, Przybyla L, Edwards T, Perera R, Burgner J, Kuhn R. Interactions of the cytoplasmic domain of Sindbis virus E2 with nucleocapsid cores promote alphavirus budding. J Virol. 2012;86:2585-99 pubmed publisher
    ..Structural studies of the Sindbis virus capsid protein (CP) have suggested that these critical interactions are mediated by the binding of cdE2 into a ..
  5. Lundstrom K. Oncolytic Alphaviruses in Cancer Immunotherapy. Vaccines (Basel). 2017;5: pubmed publisher
    ..animals were vaccinated with engineered oncolytic replication-deficient and -competent Semliki Forest virus, Sindbis virus and Venezuelan equine encephalitis virus vectors expressing various antigens...
  6. Byler K, Collins J, Ogungbe I, Setzer W. Alphavirus protease inhibitors from natural sources: A homology modeling and molecular docking investigation. Comput Biol Chem. 2016;64:163-184 pubmed publisher
    ..EEEV, VEEV, WEEV, as well as Aura virus (AURV), Barmah Forest Virus (BFV), Semliki Forest virus (SFV), and Sindbis virus (SINV) in order to identity structural scaffolds for inhibitor design or discovery...
  7. Ferguson M, Saul S, Fragkoudis R, Weisheit S, Cox J, Patabendige A, et al. Ability of the Encephalitic Arbovirus Semliki Forest Virus To Cross the Blood-Brain Barrier Is Determined by the Charge of the E2 Glycoprotein. J Virol. 2015;89:7536-49 pubmed publisher
    ..Together with studies on Sindbis virus (SINV), this study provides an important advance in understanding alphavirus, and probably other virus, ..
  8. Schneider Schaulies J, Schneider Schaulies S. Sphingolipids in viral infection. Biol Chem. 2015;396:585-95 pubmed publisher
    ..Differential activities of SMases also affect the intracellular milieu required for virus replication. Sindbis virus (SINV), for example, replicates better in cells lacking acid SMase (ASMase)...
  9. Lundberg L, Brahms A, Hooper I, Carey B, Lin S, Dahal B, et al. Repurposed FDA-Approved drug sorafenib reduces replication of Venezuelan equine encephalitis virus and other alphaviruses. Antiviral Res. 2018;157:57-67 pubmed publisher
    ..Additionally, sorafenib inhibited replication of EEEV and two Old World alphaviruses, Sindbis virus and chikungunya virus, at 8 and 16?h post-infection...
  10. Ng T, Cheung R, Wong J, Wang Y, Ip D, Wan D, et al. Antiviral activities of whey proteins. Appl Microbiol Biotechnol. 2015;99:6997-7008 pubmed publisher
    ..Lactoferrin inhibited respiratory syncytial virus, hepatitis B virus, adenovirus, poliovirus, hantavirus, sindbis virus, semliki forest virus, echovirus, and enterovirus...
  11. Lamiable O, Kellenberger C, Kemp C, Troxler L, Pelte N, Boutros M, et al. Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila. Proc Natl Acad Sci U S A. 2016;113:698-703 pubmed publisher
    ..show that die mutant flies have shortened lifespan and succumb more rapidly than controls when infected with Sindbis virus. This reduced viability is associated with deregulated activation of the immune deficiency (IMD) pathway of ..
  12. Perry J, Ahmed M, Chang K, Donato N, Showalter H, Wobus C. Antiviral activity of a small molecule deubiquitinase inhibitor occurs via induction of the unfolded protein response. PLoS Pathog. 2012;8:e1002783 pubmed publisher
    ..or induction of the UPR also reduced infection of other RNA viruses including encephalomyocarditis virus, Sindbis virus, and La Crosse virus but not vesicular stomatitis virus...
  13. Varghese F, Kaukinen P, Gläsker S, Bespalov M, Hanski L, Wennerberg K, et al. Discovery of berberine, abamectin and ivermectin as antivirals against chikungunya and other alphaviruses. Antiviral Res. 2016;126:117-24 pubmed publisher
    ..a dose-dependent manner and had broad antiviral activity against other alphaviruses--Semliki Forest virus and Sindbis virus. Abamectin and ivermectin were also active against yellow fever virus, a flavivirus...
  14. Goodman C, Russell B, Velez J, Laven J, Bagarozzi D, Moon J, et al. Production of a Sindbis/Eastern Equine Encephalitis chimeric virus inactivated cell culture antigen. J Virol Methods. 2015;223:19-24 pubmed publisher
    ..A recombinant Sindbis virus (SINV)/EEEV has been constructed for use under BSL-2 conditions and is not regulated as a select agent...
  15. Goubau D, van der Veen A, Chakravarty P, Lin R, Rogers N, Rehwinkel J, et al. Mouse superkiller-2-like helicase DDX60 is dispensable for type I IFN induction and immunity to multiple viruses. Eur J Immunol. 2015;45:3386-403 pubmed publisher
    ..murine cells or mice in resistance to infection with influenza A virus, encephalomyocarditis virus, Sindbis virus, vaccinia virus, or herpes simplex virus-1...
  16. van Mierlo J, Overheul G, Obadia B, van Cleef K, Webster C, Saleh M, et al. Novel Drosophila viruses encode host-specific suppressors of RNAi. PLoS Pathog. 2014;10:e1004256 pubmed publisher
    ..interaction is reflected in the ability of DimmNV VP1 to enhance RNA production by a recombinant Sindbis virus in a host-specific manner...
  17. Wang Y, Lu J, Lin C, Chin Y, Wu T, Lin L, et al. Antiviral activities of niclosamide and nitazoxanide against chikungunya virus entry and transmission. Antiviral Res. 2016;135:81-90 pubmed publisher
    ..possess broad anti-alphavius activities, including against two clinical CHIKV isolates and two alphaviruses: Sindbis virus (SINV) and Semliki forest virus (SFV)...
  18. Barakat A, Smura T, Kuivanen S, Huhtamo E, Kurkela S, Putkuri N, et al. The Presence and Seroprevalence of Arthropod-Borne Viruses in Nasiriyah Governorate, Southern Iraq: A Cross-Sectional Study. Am J Trop Med Hyg. 2016;94:794-9 pubmed publisher
    ..6%), sandfly-borne Sicilian virus serocomplex (18.2%), sandfly-borne Naples virus serocomplex (7.8%), Sindbis virus (1.5%), chikungunya virus (0.5%), and Tahyna virus (2.0%)...
  19. Ho Y, Liu F, Yeh C, Yang C, Lin C, Lin T, et al. Micafungin is a novel anti-viral agent of chikungunya virus through multiple mechanisms. Antiviral Res. 2018;159:134-142 pubmed publisher
    ..The efficacy of micafungin was further confirmed against two clinical isolates of CHIKV and two alphaviruses: Sindbis virus (SINV) and Semliki Forest virus (SFV)...
  20. Cheung Y, Chen K, Chen H, Seng E, Chu J. Antiviral activity of lanatoside C against dengue virus infection. Antiviral Res. 2014;111:93-9 pubmed publisher
    ..C can effectively inhibit all four serotypes of dengue virus, flavivirus Kunjin, alphavirus Chikungunya and Sindbis virus as well as the human enterovirus 71...
  21. Neris R, Figueiredo C, Higa L, Araujo D, Carvalho C, Verçoza B, et al. Co-protoporphyrin IX and Sn-protoporphyrin IX inactivate Zika, Chikungunya and other arboviruses by targeting the viral envelope. Sci Rep. 2018;8:9805 pubmed publisher
    ..we demonstrate that the antiviral activity of these porphyrins can be broadened to CHIKV, ZIKV, Mayaro virus, Sindbis virus and Vesicular Stomatitis virus...
  22. Miesen P, Girardi E, Van Rij R. Distinct sets of PIWI proteins produce arbovirus and transposon-derived piRNAs in Aedes aegypti mosquito cells. Nucleic Acids Res. 2015;43:6545-56 pubmed publisher
    ..show that Piwi5 and Ago3, but none of the other PIWI family members, are essential for piRNA biogenesis from Sindbis virus RNA in infected Aedes aegypti cells...
  23. Lawrence R, Zook J, Hogue B. Full inactivation of alphaviruses in single particle and crystallized forms. J Virol Methods. 2016;236:237-244 pubmed publisher
    ..and transmission electron microscopy, were used to demonstrate that the glutaraldehyde and UV inactivated Sindbis virus particles remain intact structurally...
  24. Lee M, Sumpter R, Zou Z, Sirasanagandla S, Wei Y, Mishra P, et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep. 2017;18:48-60 pubmed publisher
    ..Here, we demonstrate that PEX13 is required for selective autophagy of Sindbis virus (virophagy) and of damaged mitochondria (mitophagy) and that disease-associated PEX13 mutants I326T and W313G ..
  25. Li X, Meng K, Qiao J, Liu H, Zhong C, Liu Q. Identification of Aadnr1, a novel gene related to innate immunity and apoptosis in Aedes albopictus. Gene. 2016;587:18-26 pubmed publisher
    ..While when C6/36 cells were infected with Sindbis virus TE/GFP, transcriptional level of Aadnr1 was reduced and recovered repeatedly, with an overall decreasing trend...
  26. Singh H, Mudgal R, Narwal M, Kaur R, Singh V, Malik A, et al. Chikungunya virus inhibition by peptidomimetic inhibitors targeting virus-specific cysteine protease. Biochimie. 2018;149:51-61 pubmed publisher
    ..Intriguingly, these compounds did not show inhibitory effect on Sindbis virus. This suggests that Pep-I and Pep-II compounds identified as CHIKV nsP2 substrate peptidomimetics, ..
  27. Hesson J, Schafer M, Lundstrom J. First report on human-biting Culex pipiens in Sweden. Parasit Vectors. 2016;9:632 pubmed
    Culex mosquitoes are vectors of several bird-hosted arboviruses that cause outbreaks in Europe, such as Sindbis virus and West Nile virus. Recently, the human-biting form of Culex pipiens, Cx...
  28. Cihan Üstündağ G, Gürsoy E, Naesens L, Ulusoy Güzeldemirci N, Çapan G. Synthesis and antiviral properties of novel indole-based thiosemicarbazides and 4-thiazolidinones. Bioorg Med Chem. 2016;24:240-6 pubmed publisher
    ..Besides, 6b, 6c and 6d also inhibited the replication of two other RNA viruses, Sindbis virus and respiratory syncytial virus, although these EC50 values were higher compared to those noted for Coxsackie ..
  29. Sem Ouilibona R, Tchetgna Simo H, Vickos U, Berthet N, Nakoune E. Full-Length Genome Sequence of a Sindbis Virus Strain Isolated from Culex cinereus in 1977 in Bozo, Central African Republic. Genome Announc. 2018;6: pubmed publisher
    We report here the complete genome sequence of a Sindbis virus (SINV) strain, ArB7761, isolated in 1977 in the Central African Republic. This strain, closely related to the Babanki virus, belongs to the SINV genotype I clade...
  30. Kulcsar K, Griffin D. T cell-derived interleukin-10 is an important regulator of the Th17 response during lethal alphavirus encephalomyelitis. J Neuroimmunol. 2016;295-296:60-7 pubmed publisher
    Neuroadapted Sindbis virus infection of mice causes T cell-mediated fatal encephalomyelitis. In the absence of IL-10, pathogenic Th17 cells are increased and disease is accelerated...
  31. Garcia Moreno M, Sanz M, Carrasco L. Initiation codon selection is accomplished by a scanning mechanism without crucial initiation factors in Sindbis virus subgenomic mRNA. RNA. 2015;21:93-112 pubmed publisher
    ..In this study, we have examined the mechanism of initiation and AUG selection in Sindbis virus (SINV) sgmRNA...
  32. Baxter V, Griffin D. Interferon gamma modulation of disease manifestation and the local antibody response to alphavirus encephalomyelitis. J Gen Virol. 2016;97:2908-2925 pubmed publisher
    Infection of mice with Sindbis virus (SINV) produces encephalomyelitis and provides a model for examination of the central nervous system (CNS) immune response to alphavirus infection...
  33. Wang X, Li M, Zhao J, Li S, Macdonald M, Rice C, et al. Sindbis Virus Can Exploit a Host Antiviral Protein To Evade Immune Surveillance. J Virol. 2016;90:10247-10258 pubmed publisher
    ..A neurovirulent Sindbis virus strain with neuroinvasive properties (SVNI) causes lethal encephalitis in mice, and its replication in ..
  34. Girardi E, Lefèvre M, Chane Woon Ming B, Paro S, Claydon B, Imler J, et al. Cross-species comparative analysis of Dicer proteins during Sindbis virus infection. Sci Rep. 2015;5:10693 pubmed publisher
    ..insight on this restriction, we compare the dicing activity of human Dicer and fly Dicer-2 in the context of Sindbis virus (SINV) infection...
  35. Wernet M, Klovstad M, Clandinin T. A Drosophila toolkit for the visualization and quantification of viral replication launched from transgenic genomes. PLoS ONE. 2014;9:e112092 pubmed publisher
    ..Here we describe a versatile Drosophila toolkit of transgenic, self-replicating genomes ('replicons') from Sindbis virus that allow rapid visualization and quantification of viral replication in vivo...
  36. Kasaraneni N, Chamoun Emanuelli A, Wright G, Chen Z. A simple strategy for retargeting lentiviral vectors to desired cell types via a disulfide-bond-forming protein-peptide pair. Sci Rep. 2018;8:10990 pubmed publisher
    ..PDZ1 was incorporated into an engineered Sindbis virus envelope protein (Sind-PDZ1) and displayed on lentiviral particles while the TEFCA pentapeptide ligand was ..
  37. Liu X, Mutso M, Utt A, Lepland A, Herrero L, Taylor A, et al. Decreased Virulence of Ross River Virus Harboring a Mutation in the First Cleavage Site of Nonstructural Polyprotein Is Caused by a Novel Mechanism Leading to Increased Production of Interferon-Inducing RNAs. MBio. 2018;9: pubmed publisher
    ..site between nonstructural protein 1 (nsP1) and nsP2 (1/2 site) modulate type I IFN induction for both RRV and Sindbis virus (SINV)...
  38. Todorova T, Bock F, Chang P. PARP13 regulates cellular mRNA post-transcriptionally and functions as a pro-apoptotic factor by destabilizing TRAILR4 transcript. Nat Commun. 2014;5:5362 pubmed publisher
    ..is an antiviral factor, active against specific RNA viruses such as murine leukaemia virus, Sindbis virus and human immunodeficiency virus...
  39. Lebl K, Zittra C, Silbermayr K, Obwaller A, Berer D, Brugger K, et al. Mosquitoes (Diptera: Culicidae) and their relevance as disease vectors in the city of Vienna, Austria. Parasitol Res. 2015;114:707-13 pubmed publisher
    ..known to be a potent vector and pathogens like West Nile virus (WNV), Usutu virus (USUV), Tahyna virus (TAHV), Sindbis virus (SINV), Plasmodium sp., and Dirofilaria repens can be transmitted by this species. Cq...
  40. Tseng Y, Lin F, Cheng S, Tscharke D, Chulakasian S, Chou C, et al. Functional analysis of the short isoform of orf virus protein OV20.0. J Virol. 2015;89:4966-79 pubmed publisher
    ..as shown by decreased phosphorylation of the translation initiation factor eIF2α subunit and protection of Sindbis virus infection against the activity of interferon (IFN)...
  41. Kozaki T, Takahama M, Misawa T, Matsuura Y, Akira S, Saitoh T. Role of zinc-finger anti-viral protein in host defense against Sindbis virus. Int Immunol. 2015;27:357-64 pubmed publisher
    ..that zinc-finger anti-viral protein (ZAP), an IFN-inducible gene, plays a critical role in the elimination of Sindbis virus (SINV) in vitro and in vivo...
  42. Stiles K, Kielian M. Role of TSPAN9 in Alphavirus Entry and Early Endosomes. J Virol. 2016;90:4289-97 pubmed publisher
    ..in human cells, we previously identified TSPAN9 as a host factor that promotes infection by the alphaviruses Sindbis virus (SINV), Semliki Forest virus (SFV), and chikungunya virus (CHIKV)...
  43. Rajic Z, Beus M, Michnova H, Vlainić J, Persoons L, Kosalec I, et al. Asymmetric Primaquine and Halogenaniline Fumardiamides as Novel Biologically Active Michael Acceptors. Molecules. 2018;23: pubmed publisher
    ..fumardiamides 4e and 4c showed significant antiviral activity against reovirus-1, sindbis virus and Punta Toro virus (EC50 = 3.1⁻5...
  44. Nasar F, Gorchakov R, Tesh R, Weaver S. Eilat virus host range restriction is present at multiple levels of the virus life cycle. J Virol. 2015;89:1404-18 pubmed publisher
    ..Utilizing Sindbis virus (SINV) and EILV chimeras, we show that the EILV vertebrate host range restriction is also manifested at the ..
  45. Schuchman R, Kilianski A, Piper A, Vancini R, Ribeiro J, Sprague T, et al. Comparative Characterization of the Sindbis Virus Proteome from Mammalian and Invertebrate Hosts Identifies nsP2 as a Component of the Virion and Sorting Nexin 5 as a Significant Host Factor for Alphavirus Replication. J Virol. 2018;92: pubmed publisher
    ..This technology was applied to Sindbis virus, the prototypical alphavirus, to investigate the viral proteome...
  46. Muturi E, Bara J. Sindbis virus interferes with dengue 4 virus replication and its potential transmission by Aedes albopictus. Parasit Vectors. 2015;8:65 pubmed publisher
    ..This study examined how two model viruses: Sindbis virus (SINV, Togaviridae: Alphavirus) and dengue-4 virus (DENV-4, Flaviviridae: Flavivirus) may interact in C6/36 ..
  47. LaPointe A, Gebhart N, Meller M, Hardy R, Sokoloski K. Identification and Characterization of Sindbis Virus RNA-Host Protein Interactions. J Virol. 2018;92: pubmed publisher
    ..The data presented here introduce a robust viral RNA-protein discovery method to elucidate the Sindbis virus (SINV) RNA-protein host interface...
  48. Chtarbanova S, Lamiable O, Lee K, Galiana D, Troxler L, Meignin C, et al. Drosophila C virus systemic infection leads to intestinal obstruction. J Virol. 2014;88:14057-69 pubmed publisher
    ..of the transcriptome of flies infected with DCV or two other positive-sense RNA viruses, Flock House virus and Sindbis virus, reveals that DCV infection, unlike those of the other two viruses, represses the expression of a large number ..
  49. Takenouchi A, Saito K, Saito E, Saito T, Hishiki T, Matsunaga T, et al. Oncolytic viral therapy for neuroblastoma cells with Sindbis virus AR339 strain. Pediatr Surg Int. 2015;31:1151-9 pubmed publisher
    ..Oncolytic viral therapy uses replication-competent viruses, like Sindbis virus (SINV), to kill cancers. The SINV AR339 strain is blood borne and relatively non-virulent...
  50. Lwande O, Obanda V, Bucht G, Mosomtai G, Otieno V, Ahlm C, et al. Global emergence of Alphaviruses that cause arthritis in humans. Infect Ecol Epidemiol. 2015;5:29853 pubmed publisher
    ..in the emergence of Alphaviruses because these viruses, including Chikungunya virus, O'nyong'nyong virus, Sindbis virus, Ross River virus, and Mayaro virus, have caused outbreaks in Africa, Asia, Australia, Europe, and America...
  51. Kojin B, Costa da Silva A, Maciel C, Henriques D, Carvalho D, Martin K, et al. Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. Malar J. 2016;15:153 pubmed publisher
    ..and Plasmodium falciparum, respectively, were expressed endogenously in mosquito haemolymph using a transient (Sindbis virus-mediated) or stable (piggyBac-mediated transgenesis) system...
  52. Walker B, Hengst U, Kim H, Jeon N, Schmidt E, Heintz N, et al. Reprogramming axonal behavior by axon-specific viral transduction. Gene Ther. 2012;19:947-55 pubmed publisher
    ..To use axonal ribosomes to alter local protein expression, we utilized a Sindbis virus containing an RNA genome that has been modified so that it can be directly used as a template for translation...
  53. Zhu W, Deng L, Wei Y, Wang H, Wang J, Liang G. A substitution in nsP1 combined with a double substitution in E2 glycoprotein renders Sindbis-like virus XJ-160 fully neurovirulent for adult mice. Virus Res. 2015;196:1-4 pubmed publisher
    b>Sindbis virus (SV) can be rendered neurovirulent for adult mice by a double substitution within the E2 glycoprotein, including replacing Gln at position 55 of E2 with a His (E2-55: Gln-His) and E2-70: Glu to Lys...
  54. Ding J, Lawrence R, Jones P, Hogue B, Hayes M. Concentration of Sindbis virus with optimized gradient insulator-based dielectrophoresis. Analyst. 2016;141:1997-2008 pubmed publisher
    ..In this work, an optimized gradient insulator-based DEP device was utilized for concentration of Sindbis virus, an animal virus with a diameter of 68 nm...
  55. Lulla V, Karo Astover L, Rausalu K, Saul S, Merits A, Lulla A. Timeliness of Proteolytic Events Is Prerequisite for Efficient Functioning of the Alphaviral Replicase. J Virol. 2018;92: pubmed publisher
    ..We also extended our conclusions to Sindbis virus by analyzing the properties of the hyperprocessive variant carrying the N614D mutation in nsP2...
  56. Xu J, Nash R, Frey T. Cellular responses to Sindbis virus infection of neural progenitors derived from human embryonic stem cells. BMC Res Notes. 2014;7:757 pubmed publisher
    b>Sindbis virus (SINV) causes age-dependent encephalitis in mice, and therefore serves as a model to study viral encephalitis...
  57. Xiao X, Zhang R, Pang X, Liang G, Wang P, Cheng G. A neuron-specific antiviral mechanism prevents lethal flaviviral infection of mosquitoes. PLoS Pathog. 2015;11:e1004848 pubmed publisher
    ..Functional blockade of AaHig enhanced Dengue virus (DENV) and Japanese encephalitis virus (JEV), but not Sindbis virus (SINV), replication in mosquito heads and consequently caused neural apoptosis and a dramatic reduction in the ..
  58. Poirier E, Mounce B, Rozen Gagnon K, Hooikaas P, Stapleford K, Moratorio G, et al. Low-Fidelity Polymerases of Alphaviruses Recombine at Higher Rates To Overproduce Defective Interfering Particles. J Virol. 2015;90:2446-54 pubmed publisher
    ..We hypothesized that fidelity, recombination, and DI production are tightly linked. We show that a Sindbis virus mutator replicating at a high multiplicity of infection manifests an earlier and greater accumulation of DIs ..
  59. Mackenzie Liu D, Sokoloski K, Purdy S, Hardy R. Encapsidated Host Factors in Alphavirus Particles Influence Midgut Infection of Aedes aegypti. Viruses. 2018;10: pubmed publisher
    ..The infectivity of Sindbis virus (SINV), the type species of the Alphavirus genus, is influenced by both the original and new host cell...
  60. Sigei F, Nindo F, Mukunzi S, Ng ang a Z, Sang R. Evolutionary analyses of Sindbis virus strains isolated from mosquitoes in Kenya. Arch Virol. 2018;163:2465-2469 pubmed publisher
    b>Sindbis virus (SINV) is a mosquito borne virus maintained in nature in a mosquito-bird cycle, with human outbreaks known to occur in Northern Europe and parts of Africa...
  61. Ruiz Guillen M, Gabev E, Quetglas J, Casales E, Ballesteros Briones M, Poutou J, et al. Capsid-deficient alphaviruses generate propagative infectious microvesicles at the plasma membrane. Cell Mol Life Sci. 2016;73:3897-916 pubmed publisher
    ..In the present study, we show that Semliki Forest virus and Sindbis virus lacking the capsid gene can propagate in mammalian and insect cells...
  62. Li M, Wang H, Stollar V. In vitro synthesis of Sindbis virus genomic and subgenomic RNAs: influence of nsP4 mutations and nucleoside triphosphate concentrations. J Virol. 2010;84:2732-9 pubmed publisher
    Two positive-strand mRNAs are made in Sindbis virus-infected cells, the genomic (G) RNA and the subgenomic (SG) RNA...
  63. Gencoglu M, Heldt C. Enveloped virus flocculation and removal in osmolyte solutions. J Biotechnol. 2015;206:8-11 pubmed publisher
    ..In this work we show that osmolytes flocculate the enveloped virus, Sindbis virus heat resistant strain (SVHR), and demonstrate a >80% removal with a 0...
  64. Sali T, Pryke K, Abraham J, Liu A, Archer I, Broeckel R, et al. Characterization of a Novel Human-Specific STING Agonist that Elicits Antiviral Activity Against Emerging Alphaviruses. PLoS Pathog. 2015;11:e1005324 pubmed publisher
    ..of emerging Alphavirus species including Chikungunya virus, Venezuelan Equine Encephalitis virus, and Sindbis virus. To define cellular proteins essential to elicitation of the antiviral activity by the compound we employed a ..
  65. Nilaratanakul V, Chen J, Tran O, Baxter V, Troisi E, Yeh J, et al. Germ Line IgM Is Sufficient, but Not Required, for Antibody-Mediated Alphavirus Clearance from the Central Nervous System. J Virol. 2018;92: pubmed publisher
    b>Sindbis virus (SINV) infection of neurons in the brain and spinal cord in mice provides a model system for investigating recovery from encephalomyelitis and antibody-mediated clearance of virus from the central nervous system (CNS)...
  66. Kulcsar K, Baxter V, Abraham R, Nelson A, Griffin D. Distinct Immune Responses in Resistant and Susceptible Strains of Mice during Neurovirulent Alphavirus Encephalomyelitis. J Virol. 2015;89:8280-91 pubmed publisher
    ..Neuroadapted Sindbis virus (NSV) causes uniformly fatal disease in adult C57BL/6 (B6) mice, but adult BALB/c (Bc) mice recover from ..
  67. Jose J, Tang J, Taylor A, Baker T, Kuhn R. Fluorescent Protein-Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus Budding from Live Cells. Viruses. 2015;7:6182-99 pubmed publisher
    b>Sindbis virus (SINV) is an enveloped, mosquito-borne alphavirus...
  68. Li Y, Banerjee S, Wang Y, Goldstein S, Dong B, Gaughan C, et al. Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proc Natl Acad Sci U S A. 2016;113:2241-6 pubmed publisher
    ..surrogate for viral dsRNA, or infection with each of four viruses from different groups (West Nile virus, Sindbis virus, influenza virus, or vaccinia virus), OAS1-KO and OAS2-KO cells synthesized amounts of 2-5A similar to those ..
  69. Panda D, Gold B, Tartell M, Rausch K, Casas Tinto S, Cherry S. The transcription factor FoxK participates with Nup98 to regulate antiviral gene expression. MBio. 2015;6: pubmed publisher
    ..RNAi) screen, we identified a relatively understudied forkhead transcription factor, FoxK, as active against Sindbis virus (SINV) in Drosophila...
  70. Albulescu I, van Hoolwerff M, Wolters L, Bottaro E, Nastruzzi C, Yang S, et al. Suramin inhibits chikungunya virus replication through multiple mechanisms. Antiviral Res. 2015;121:39-46 pubmed publisher
    ..various CHIKV isolates in cell culture with an EC50 of ∼80μM (CC50>5mM) and was also active against Sindbis virus and Semliki Forest virus...
  71. Carrasco L, Sanz M, González Almela E. The Regulation of Translation in Alphavirus-Infected Cells. Viruses. 2018;10: pubmed publisher
    b>Sindbis virus (SINV) contains an RNA genome of positive polarity with two open reading frames (ORFs)...
  72. Jose J, Taylor A, Kuhn R. Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells. MBio. 2017;8: pubmed publisher
    b>Sindbis virus (SINV [genus Alphavirus, family Togaviridae]) is an enveloped, mosquito-borne virus...
  73. O Neill K, Olson B, Huang N, Unis D, Clem R. Rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector. Proc Natl Acad Sci U S A. 2015;112:E1152-61 pubmed publisher
    ..the effect of stimulating apoptosis during arbovirus infection by infecting Aedes aegypti mosquitoes with a Sindbis virus (SINV) clone called MRE/Rpr, in which the MRE-16 strain of SINV was engineered to express the proapoptotic ..
  74. Sokoloski K, Haist K, Morrison T, Mukhopadhyay S, Hardy R. Noncapped Alphavirus Genomic RNAs and Their Role during Infection. J Virol. 2015;89:6080-92 pubmed publisher
    ..Previously we have reported that the infectivity of Sindbis virus (SINV), the model alphavirus, was largely a function of the cell line producing the viral particles...
  75. Yi R, Saito K, Isegawa N, Shirasawa H. Alteration of cell cycle progression by Sindbis virus infection. Biochem Biophys Res Commun. 2015;462:426-32 pubmed publisher
    We examined the impact of Sindbis virus (SINV) infection on cell cycle progression in a cancer cell line, HeLa, and a non-cancerous cell line, Vero...
  76. González Almela E, Sanz M, García Moreno M, Northcote P, Pelletier J, Carrasco L. Differential action of pateamine A on translation of genomic and subgenomic mRNAs from Sindbis virus. Virology. 2015;484:41-50 pubmed publisher
    ..In the present study, we have examined the activity of Pat A on the translation of Sindbis virus (SINV) mRNAs...
  77. Martin N, Griffin D. Interleukin-10 Modulation of Virus Clearance and Disease in Mice with Alphaviral Encephalomyelitis. J Virol. 2018;92: pubmed publisher
    ..Previous studies in mice with a virulent strain (neuroadapted SINV [NSV]) of the alphavirus Sindbis virus (SINV) identified a role for Th17 cells and regulation by interleukin-10 (IL-10) in the pathogenesis of fatal ..
  78. Morley V, Mendiola S, Turner P. Rate of novel host invasion affects adaptability of evolving RNA virus lineages. Proc Biol Sci. 2015;282:20150801 pubmed publisher
    ..To test whether the rate of environmental turnover influences adaptation, we experimentally evolved 144 Sindbis virus lineages in replicated tissue-culture environments, which transitioned from being dominated by a permissive ..
  79. Feibelman K, Fuller B, Li L, Labarbera D, Geiss B. Identification of small molecule inhibitors of the Chikungunya virus nsP1 RNA capping enzyme. Antiviral Res. 2018;154:124-131 pubmed publisher
    ..In addition, live virus assays with CHIKV and closely related alphavirus, Sindbis virus, were used in conjunction with cell toxicity assays to determine the antiviral activity of compounds in cell ..
  80. Goussen C, Simoneau S, Bérend S, Jehan Kimmel C, Bellon A, Ducloux C, et al. Biological Safety of a Highly Purified 10% Liquid Intravenous Immunoglobulin Preparation from Human Plasma. BioDrugs. 2017;31:251-261 pubmed publisher
    ..9 log10 for bovine viral diarrhoea virus (BVDV)/Sindbis virus, ≥15.7 log10 for pseudorabies virus (PRV), ≥12...
  81. Calzolari M, Bonilauri P, Bellini R, Becker S, Dottori M. Wide recognition of Culex pipiens and lack of detection of Culex torrentium through biomolecular differentiation of mosquitoes in the Emilia-Romagna region, Northern Italy. Med Vet Entomol. 2016;30:435-438 pubmed publisher
    ..viruses, many of which are of significant importance to human health such as the West Nile virus and the Sindbis virus. This group of mosquitoes is difficult to distinguish morphologically; particularly as adult females...
  82. Passoni G, Langevin C, Palha N, Mounce B, Briolat V, Affaticati P, et al. Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes. Dis Model Mech. 2017;10:847-857 pubmed publisher
    Alphaviruses, such as chikungunya virus (CHIKV) and Sindbis virus (SINV), are vector-borne pathogens that cause acute illnesses in humans and are sometimes associated with neuropathies, especially in infants and elderly patients...
  83. Ou J, Trent D, Strauss J. The 3'-non-coding regions of alphavirus RNAs contain repeating sequences. J Mol Biol. 1982;156:719-30 pubmed
  84. Ramsey J, Renzi E, Arnold R, Trinidad J, Mukhopadhyay S. Palmitoylation of Sindbis Virus TF Protein Regulates Its Plasma Membrane Localization and Subsequent Incorporation into Virions. J Virol. 2017;91: pubmed publisher
    ..In this study, we used cysteine (Cys) mutants to test differential palmitoylation of the Sindbis virus 6K and TF proteins...
  85. Li M, Bozzacco L, Hoffmann H, Breton G, Loschko J, Xiao J, et al. Interferon regulatory factor 2 protects mice from lethal viral neuroinvasion. J Exp Med. 2016;213:2931-2947 pubmed
    ..A Sindbis virus strain that in wild-type (WT) mice only causes disease when injected into the brain leads to lethal ..
  86. Zheng X, Wang X, Tu F, Wang Q, Fan Z, Gao G. TRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein. J Virol. 2017;91: pubmed publisher
    ..ZAP is a host antiviral factor that specifically inhibits the replication of certain viruses, including HIV-1, Sindbis virus, and Ebola virus...
  87. Bhattacharya T, Newton I, Hardy R. Wolbachia elevates host methyltransferase expression to block an RNA virus early during infection. PLoS Pathog. 2017;13:e1006427 pubmed publisher
    ..To this end, we have established a robust arthropod model system to study the tripartite interaction involving Sindbis virus and Wolbachia strain wMel within its native host, Drosophila melanogaster...
  88. Wada Y, Orba Y, Sasaki M, Kobayashi S, Carr M, Nobori H, et al. Discovery of a novel antiviral agent targeting the nonstructural protein 4 (nsP4) of chikungunya virus. Virology. 2017;505:102-112 pubmed publisher
    ..compound designated Compound-A was found to inhibit infection by several CHIKV strains and a Sindbis virus strain at nanomolar concentrations...
  89. Lundstrom K. Alphavirus-Based Vaccines. Methods Mol Biol. 2017;1581:225-242 pubmed publisher
    Alphavirus-based vectors have been engineered from Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus and applied for vaccine development...
  90. Snyder J, Kulcsar K, Schultz K, Riley C, Neary J, Marr S, et al. Functional characterization of the alphavirus TF protein. J Virol. 2013;87:8511-23 pubmed publisher
    ..We next constructed a panel of Sindbis virus mutants with mutations which alter the production, size, or sequence of TF...
  91. Kozaki T, Komano J, Kanbayashi D, Takahama M, Misawa T, Satoh T, et al. Mitochondrial damage elicits a TCDD-inducible poly(ADP-ribose) polymerase-mediated antiviral response. Proc Natl Acad Sci U S A. 2017;114:2681-2686 pubmed publisher
    ..ribose) polymerase (TIPARP), a Cysteine3 Histidine (CCCH)-type zinc finger-containing protein, binds to Sindbis virus (SINV) RNA via its zinc finger domain and recruits an exosome to induce viral RNA degradation...
  92. Huangfu C, Ma Y, Jia J, Lv M, Zhu F, Ma X, et al. Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of ?2-Macroglobulin from Cohn Fraction IV. Biologicals. 2017;46:139-142 pubmed publisher
    ..By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5.88 log10, 7...