Semliki forest virus

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Top Publications

  1. Chanel Vos C, Kielian M. A conserved histidine in the ij loop of the Semliki Forest virus E1 protein plays an important role in membrane fusion. J Virol. 2004;78:13543-52 pubmed publisher
    The enveloped alphavirus Semliki Forest virus (SFV) infects cells via a low pH-triggered membrane fusion reaction mediated by the E1 protein...
  2. Takkinen K. Complete nucleotide sequence of the nonstructural protein genes of Semliki Forest virus. Nucleic Acids Res. 1986;14:5667-82 pubmed
    The nucleotide sequence coding for the nonstructural proteins of Semliki Forest virus has been determined from cDNA clones...
  3. Garoff H, Frischauf A, Simons K, Lehrach H, Delius H. Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins. Nature. 1980;288:236-41 pubmed
    The genes coding for the three membrane polypeptides of Semliki Forest virus have been sequenced and the primary structures of the proteins deduced...
  4. Salonen A, Vasiljeva L, Merits A, Magden J, Jokitalo E, Kääriäinen L. Properly folded nonstructural polyprotein directs the semliki forest virus replication complex to the endosomal compartment. J Virol. 2003;77:1691-702 pubmed
    ..Virol. 75:3873-3884, 2001). To understand how CPVs arise, we have expressed the individual Semliki Forest virus (SFV) nonstructural proteins nsP1 to nsP4 in different combinations, as well as their precursor polyprotein ..
  5. McInerney G, Smit J, Liljestrom P, Wilschut J. Semliki Forest virus produced in the absence of the 6K protein has an altered spike structure as revealed by decreased membrane fusion capacity. Virology. 2004;325:200-6 pubmed
    We examined the kinetics of membrane fusion of wild type (wt) and Delta6K mutant Semliki Forest virus in a liposomal model system. The final extent of membrane fusion of the mutant (at pH 5...
  6. Zhang X, Kielian M. Mutations that promote furin-independent growth of Semliki Forest virus affect p62-E1 interactions and membrane fusion. Virology. 2004;327:287-96 pubmed publisher
    The enveloped alphavirus Semliki Forest virus (SFV) infects cells via a low pH-triggered membrane fusion reaction mediated by the E1 protein...
  7. Roussel A, Lescar J, Vaney M, Wengler G, Wengler G, Rey F. Structure and interactions at the viral surface of the envelope protein E1 of Semliki Forest virus. Structure. 2006;14:75-86 pubmed publisher
    b>Semliki Forest virus (SFV) is enveloped by a lipid bilayer enclosed within a glycoprotein cage made by glycoproteins E1 and E2...
  8. Lulla V, Merits A, Sarin P, K ri inen L, Ker nen S, Ahola T. Identification of mutations causing temperature-sensitive defects in Semliki Forest virus RNA synthesis. J Virol. 2006;80:3108-11 pubmed publisher
    We have sequenced the nonstructural protein coding region of Semliki Forest virus temperature-sensitive (ts) mutant strains ts1, ts6, ts9, ts10, ts11, ts13, and ts14...
  9. Galbraith S, Sheahan B, Atkins G. Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence. J Gen Virol. 2006;87:937-47 pubmed publisher
    Major virulence determinants of Semliki Forest virus (SFV) lie within the non-structural genes that form the replicase complex proteins...

More Information

Publications118 found, 100 shown here

  1. Chanel Vos C, Kielian M. Second-site revertants of a Semliki Forest virus fusion-block mutation reveal the dynamics of a class II membrane fusion protein. J Virol. 2006;80:6115-22 pubmed publisher
    The alphavirus Semliki Forest virus (SFV) infects cells through low-pH-induced membrane fusion mediated by the E1 protein, a class II virus membrane fusion protein...
  2. Liao M, Kielian M. Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly. J Virol. 2006;80:11362-9 pubmed publisher
    ..Mutagenesis studies of the alphavirus Semliki Forest virus (SFV) here demonstrated that there was a strong requirement for the E1 stem in virus assembly and budding, ..
  3. Spuul P, Salonen A, Merits A, Jokitalo E, K ri inen L, Ahola T. Role of the amphipathic peptide of Semliki forest virus replicase protein nsP1 in membrane association and virus replication. J Virol. 2007;81:872-83 pubmed publisher
    b>Semliki Forest virus RNA replication takes place in association with specific cytoplasmic vacuoles, derived from the endosomal apparatus...
  4. Breakwell L, Dosenovic P, Karlsson Hedestam G, D Amato M, Liljestrom P, Fazakerley J, et al. Semliki Forest virus nonstructural protein 2 is involved in suppression of the type I interferon response. J Virol. 2007;81:8677-84 pubmed
    ..b>Semliki Forest virus (SFV) infection induces rapid and profound silencing of host cell gene expression, a process believed to be ..
  5. Markosyan R, Kielian M, Cohen F. Fusion induced by a class II viral fusion protein, semliki forest virus E1, is dependent on the voltage of the target cell. J Virol. 2007;81:11218-25 pubmed publisher
    Cells expressing the low pH-triggered class II viral fusion protein E1 of Semliki Forest virus (SFV) were fused to target cells. Fusion was monitored by electrical capacitance and aqueous dye measurements...
  6. Tamm K, Merits A, Sarand I. Mutations in the nuclear localization signal of nsP2 influencing RNA synthesis, protein expression and cytotoxicity of Semliki Forest virus. J Gen Virol. 2008;89:676-86 pubmed publisher
    The cytotoxicity of Semliki Forest virus (SFV) infection is caused partly by the non-structural protein nsP2, an essential component of the SFV replicase complex...
  7. Qin Z, Zheng Y, Kielian M. Role of conserved histidine residues in the low-pH dependence of the Semliki Forest virus fusion protein. J Virol. 2009;83:4670-7 pubmed publisher
    ..For alphaviruses such as Semliki Forest virus (SFV), acidic pH initiates a series of conformational changes in the heterodimeric virus envelope proteins ..
  8. Liu C, Kielian M. E1 mutants identify a critical region in the trimer interface of the Semliki forest virus fusion protein. J Virol. 2009;83:11298-306 pubmed publisher
    The alphavirus Semliki Forest virus (SFV) uses a membrane fusion reaction to infect host cells. Fusion of the virus and cell membranes is triggered by low pH in the endosome and is mediated by the viral membrane protein E1...
  9. Varjak M, Zusinaite E, Merits A. Novel functions of the alphavirus nonstructural protein nsP3 C-terminal region. J Virol. 2010;84:2352-64 pubmed publisher
    ..A functional analysis of the C-terminal region of nsP3 of Semliki Forest virus revealed the presence of a degradation signal that overlaps with a sequence element located between nsP3 ..
  10. Liu C, Besanceney C, Song Y, Kielian M. Pseudorevertants of a Semliki forest virus fusion-blocking mutation reveal a critical interchain interaction in the core trimer. J Virol. 2010;84:11624-33 pubmed publisher
    b>Semliki Forest virus (SFV) is an enveloped alphavirus that infects cells by a low-pH-triggered membrane fusion reaction mediated by the viral E1 protein...
  11. Lulla A, Lulla V, Merits A. Macromolecular assembly-driven processing of the 2/3 cleavage site in the alphavirus replicase polyprotein. J Virol. 2012;86:553-65 pubmed publisher
    b>Semliki Forest virus (SFV) is a member of the Alphavirus genus, which produces its replicase proteins in the form of a nonstructural (ns) polyprotein precursor P1234...
  12. Akhrymuk I, Kulemzin S, Frolova E. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol. 2012;86:7180-91 pubmed publisher
    ..This indicates that the nsP2-induced degradation of Rpb1 is a critical mechanism utilized by the Old World alphaviruses to subvert the cellular antiviral response...
  13. Panas M, Varjak M, Lulla A, Eng K, Merits A, Karlsson Hedestam G, et al. Sequestration of G3BP coupled with efficient translation inhibits stress granules in Semliki Forest virus infection. Mol Biol Cell. 2012;23:4701-12 pubmed publisher
    ..Here, we report that early in Semliki Forest virus infection, the C-terminal domain of the viral nonstructural protein 3 (nsP3) forms a complex with Ras-GAP ..
  14. Wu S, Haag L, Hammar L, Wu B, Garoff H, Xing L, et al. The dynamic envelope of a fusion class II virus. Prefusion stages of semliki forest virus revealed by electron cryomicroscopy. J Biol Chem. 2007;282:6752-62 pubmed publisher
    b>Semliki Forest virus is among the prototypes for Class II virus fusion and targets the endosomal membrane...
  15. Lulla V, Sawicki D, Sawicki S, Lulla A, Merits A, Ahola T. Molecular defects caused by temperature-sensitive mutations in Semliki Forest virus nsP1. J Virol. 2008;82:9236-44 pubmed publisher
    ..Two temperature-sensitive (ts) mutations in Semliki Forest virus (SFV) were previously identified within nsP1: ts10 (E529D) and ts14 (D119N)...
  16. Jia F, Zhu X, Lv P, Hu L, Liu Q, Jin S, et al. Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus. Neurosci Bull. 2019;: pubmed publisher
    ..for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0...
  17. Younis S, Kamel W, Falkeborn T, Wang H, Yu D, Daniels R, et al. Multiple nuclear-replicating viruses require the stress-induced protein ZC3H11A for efficient growth. Proc Natl Acad Sci U S A. 2018;115:E3808-E3816 pubmed publisher
    ..herpes simplex virus, and adenovirus), whereas cytoplasmic replicating viruses are not (vaccinia virus and Semliki Forest virus)...
  18. Lulla V, Karo Astover L, Rausalu K, Saul S, Merits A, Lulla A. Timeliness of Proteolytic Events Is Prerequisite for Efficient Functioning of the Alphaviral Replicase. J Virol. 2018;92: pubmed publisher
    ..In this study, we analyzed the consequences of the artificially accelerated processing of the Semliki Forest virus ns-polyprotein...
  19. Quirin T, Chen Y, Pietilä M, Guo D, Ahola T. The RNA Capping Enzyme Domain in Protein A is Essential for Flock House Virus Replication. Viruses. 2018;10: pubmed publisher
    The nodavirus flock house virus (FHV) and the alphavirus Semliki Forest virus (SFV) show evolutionarily intriguing similarities in their replication complexes and RNA capping enzymes...
  20. Sundeen G, Barbieri J. Vaccines against Botulism. Toxins (Basel). 2017;9: pubmed publisher
    ..Viral vectors reviewed are adenovirus, influenza virus, rabies virus, Semliki Forest virus, and Venezuelan Equine Encephalitis virus...
  21. Luca V, Nelson C, Fremont D. Structure of the St. Louis encephalitis virus postfusion envelope trimer. J Virol. 2013;87:818-28 pubmed publisher
    ..that they are positioned further apart than previously observed in flaviviruses but closer together than Semliki Forest virus, an alphavirus...
  22. Grice I, Mariottini G. Glycans with Antiviral Activity from Marine Organisms. Results Probl Cell Differ. 2018;65:439-475 pubmed publisher
    ..virus, murine sarcoma virus, Newcastle disease virus, parainfluenza virus, respiratory syncytial virus, Semliki Forest virus, tobacco mosaic virus, vaccinia virus, varicella zoster virus, viral haemorrhagic septicaemia virus and ..
  23. Donald C, Varjak M, Aguiar E, Marques J, Sreenu V, Schnettler E, et al. Antiviral RNA Interference Activity in Cells of the Predatory Mosquito, Toxorhynchites amboinensis. Viruses. 2018;10: pubmed publisher
    ..these mosquitoes possess a functional RNAi response that is active against a mosquito-borne alphavirus, Semliki Forest virus. As observed in vector mosquito species, small RNAs are produced that target viral sequences...
  24. Ho Y, Liu F, Yeh C, Yang C, Lin C, Lin T, et al. Micafungin is a novel anti-viral agent of chikungunya virus through multiple mechanisms. Antiviral Res. 2018;159:134-142 pubmed publisher
    ..was further confirmed against two clinical isolates of CHIKV and two alphaviruses: Sindbis virus (SINV) and Semliki Forest virus (SFV)...
  25. Tang J, Bi Z, Ding M, Yin D, Zhu J, Zhang L, et al. Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus. Virol J. 2018;15:140 pubmed publisher
    ..In this study, a suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon and DTMUV E glycoprotein gene was constructed and the efficacy of this new vaccine was ..
  26. Michlmayr D, McKimmie C, Pingen M, Haxton B, Mansfield K, Johnson N, et al. Defining the chemokine basis for leukocyte recruitment during viral encephalitis. J Virol. 2014;88:9553-67 pubmed publisher
    ..Here we examine chemokine expression and leukocyte recruitment in the context of avirulent and virulent Semliki Forest virus (SFV) as well as West Nile virus infection and demonstrate rapid and robust expression of a variety of ..
  27. Zhu Y, Li C, Bi Z, Chen Z, Meng C, Wang G, et al. Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus. Vet Immunol Immunopathol. 2015;165:88-92 pubmed publisher
    A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV)...
  28. Ooi Y, Dubé M, Kielian M. BST2/tetherin inhibition of alphavirus exit. Viruses. 2015;7:2147-67 pubmed publisher
    Alphaviruses such as chikungunya virus (CHIKV) and Semliki Forest virus (SFV) are small enveloped RNA viruses that bud from the plasma membrane...
  29. Autio K, Ruotsalainen J, Anttila M, Niittykoski M, Waris M, Hemminki A, et al. Attenuated Semliki Forest virus for cancer treatment in dogs: safety assessment in two laboratory Beagles. BMC Vet Res. 2015;11:170 pubmed publisher
    ..A non-pathogenic Semliki Forest virus vector VA7-EGFP previously showed promise in targeting human tumor xenografts in mice, but the oncolytic ..
  30. Yin X, Wang W, Zhu X, Wang Y, Wu S, Wang Z, et al. Synergistic antitumor efficacy of combined DNA vaccines targeting tumor cells and angiogenesis. Biochem Biophys Res Commun. 2015;465:239-44 pubmed publisher
    ..In this study, a Semliki Forest Virus (SFV) replicon DNA vaccine expressing 1-4 domains of murine VEGFR2 and IL12 was constructed, and was named ..
  31. Schnettler E, Donald C, Human S, Watson M, Siu R, McFarlane M, et al. Knockdown of piRNA pathway proteins results in enhanced Semliki Forest virus production in mosquito cells. J Gen Virol. 2013;94:1680-9 pubmed publisher
    ..Here we show that piRNA-like molecules are produced following infection with the naturally mosquito-borne Semliki Forest virus in mosquito cell lines...
  32. Varjak M, Dietrich I, Sreenu V, Till B, Merits A, Kohl A, et al. Spindle-E Acts Antivirally Against Alphaviruses in Mosquito Cells. Viruses. 2018;10: pubmed publisher
    ..replication indicating that SpnE acts as an antiviral against alphaviruses such as CHIKV and the related Semliki Forest virus (SFV). Surprisingly, this effect was found to be independent of the siRNA and piRNA pathways in Ae...
  33. Melero I, Quetglas J, Reboredo M, Dubrot J, Rodriguez Madoz J, Mancheño U, et al. Strict requirement for vector-induced type I interferon in efficacious antitumor responses to virally encoded IL12. Cancer Res. 2015;75:497-507 pubmed publisher
    ..Specifically, we used a Semliki Forest virus encoding IL12 (SFV-IL12) based on its promise as an RNA viral vector for cancer treatment...
  34. Fragkoudis R, Dixon Ballany C, Zagrajek A, Kedzierski L, Fazakerley J. Following Acute Encephalitis, Semliki Forest Virus is Undetectable in the Brain by Infectivity Assays but Functional Virus RNA Capable of Generating Infectious Virus Persists for Life. Viruses. 2018;10: pubmed publisher
    ..In the mouse, peripheral infection with Semliki Forest virus (SFV) results in encephalitis...
  35. Hellström K, Vihinen H, Kallio K, Jokitalo E, Ahola T. Correlative light and electron microscopy enables viral replication studies at the ultrastructural level. Methods. 2015;90:49-56 pubmed publisher
    ..Here we have employed CLEM for Semliki Forest virus (SFV) replication studies both by transfecting viral replication components to cells or by infecting ..
  36. Fernández Núñez E, de Rezende A, Puglia A, Leme J, Boldorini V, Caricati C, et al. Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension. Biotechnol Lett. 2015;37:1153-63 pubmed publisher
    To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension...
  37. Baxter V, Heise M. Genetic control of alphavirus pathogenesis. Mamm Genome. 2018;29:408-424 pubmed publisher
    ..clinical disease characterized by rash, arthralgia, and arthritis (chikungunya virus, Ross River virus, and Semliki Forest virus) or encephalomyelitis (eastern equine encephalitis virus, western equine encephalitis virus, and Venezuelan ..
  38. Van de Wall S, Walczak M, van Rooij N, Hoogeboom B, Meijerhof T, Nijman H, et al. Tattoo Delivery of a Semliki Forest Virus-Based Vaccine Encoding Human Papillomavirus E6 and E7. Vaccines (Basel). 2015;3:221-38 pubmed publisher
    ..In this study, we explored the efficacy of tattoo injection of a tumor vaccine based on recombinant Semliki Forest virus replicon particles (rSFV) targeting human papillomavirus (HPV)...
  39. Kallio K, Hellström K, Jokitalo E, Ahola T. RNA Replication and Membrane Modification Require the Same Functions of Alphavirus Nonstructural Proteins. J Virol. 2016;90:1687-92 pubmed publisher
    ..membrane association) essential for RNA synthesis also prevents the generation of spherule structures in a Semliki Forest virus trans-replication system...
  40. Griffiths S, Koegl M, Boutell C, Zenner H, Crump C, Pica F, et al. A systematic analysis of host factors reveals a Med23-interferon-λ regulatory axis against herpes simplex virus type 1 replication. PLoS Pathog. 2013;9:e1003514 pubmed publisher
    ..and this inhibitory effect was specific to HSV-1, as a range of other viruses including Vaccinia virus and Semliki Forest virus were unaffected by Med23 depletion...
  41. Crispin M, Harvey D, Bitto D, Bonomelli C, Edgeworth M, Scrivens J, et al. Structural plasticity of the Semliki Forest virus glycome upon interspecies transmission. J Proteome Res. 2014;13:1702-12 pubmed publisher
    ..Alphavirus biogenesis has been extensively studied, and the Semliki Forest virus E1 and E2 glycoproteins have been shown to exhibit differing degrees of processing of N-linked glycans...
  42. Varghese F, Kaukinen P, Gläsker S, Bespalov M, Hanski L, Wennerberg K, et al. Discovery of berberine, abamectin and ivermectin as antivirals against chikungunya and other alphaviruses. Antiviral Res. 2016;126:117-24 pubmed publisher
    ..CHIKV replication in a dose-dependent manner and had broad antiviral activity against other alphaviruses--Semliki Forest virus and Sindbis virus. Abamectin and ivermectin were also active against yellow fever virus, a flavivirus...
  43. van den Pol A, Mao G, Chattopadhyay A, Rose J, Davis J. Chikungunya, Influenza, Nipah, and Semliki Forest Chimeric Viruses with Vesicular Stomatitis Virus: Actions in the Brain. J Virol. 2017;91: pubmed publisher
    ..in which the VSV glycoprotein gene is expressed from a replicon encoding the nonstructural proteins of Semliki Forest virus. VSVΔG-CHIKV, VSVΔG-H5N1, and VLV were all safe in the adult mouse brain, as were VSVΔG viruses ..
  44. Moniuszko A, Rückert C, Alberdi M, Barry G, Stevenson B, Fazakerley J, et al. Coinfection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens. Ticks Tick Borne Dis. 2014;5:415-22 pubmed publisher
    ..s., Ehrlichia ruminantium, or Semliki Forest virus (SFV), and the effect of coinfection on the replication of these pathogens was measured...
  45. Levanov L, Jääskeläinen A, Vapalahti O. Test based on subtype-specific μ-capture IgM immunoassay can distinguish between infections of European and Siberian subtypes of tick-borne encephalitis virus. J Clin Virol. 2015;73:81-83 pubmed publisher
    ..5. We conclude that the assay is a useful tool to distinguish between acute infections of European and Siberian strains of TBEV, and should help in further studies of the clinical outcome of these two subtypes. ..
  46. Karo Astover L, Sarova O, Merits A, Zusinaite E. The infection of mammalian and insect cells with SFV bearing nsP1 palmitoylation mutations. Virus Res. 2010;153:277-87 pubmed publisher
    b>Semliki Forest virus (SFV), an alphavirus, replicates in vertebrate host and mosquito vector cells...
  47. Draghiciu O, Nijman H, Hoogeboom B, Meijerhof T, Daemen T. Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication. Oncoimmunology. 2015;4:e989764 pubmed
    ..or in combination with intramuscular immunization using a viral vector based cancer vaccine composed of Semliki Forest virus replicon particles and encoding the oncoproteins E6 and E7 (SFVeE6,7) of human papilloma virus (HPV)...
  48. Weger Lucarelli J, Aliota M, Wlodarchak N, Kamlangdee A, Swanson R, Osorio J. Dissecting the Role of E2 Protein Domains in Alphavirus Pathogenicity. J Virol. 2015;90:2418-33 pubmed publisher
    ..In contrast to CHIKV, other related alphaviruses, such as Venezuelan equine encephalitis virus (VEEV) and Semliki Forest virus (SFV), can cause encephalitic disease...
  49. Papaianni E, El Maadidi S, Schejtman A, Neumann S, Maurer U, Marino Merlo F, et al. Phylogenetically Distant Viruses Use the Same BH3-Only Protein Puma to Trigger Bax/Bak-Dependent Apoptosis of Infected Mouse and Human Cells. PLoS ONE. 2015;10:e0126645 pubmed publisher
    ..double stranded DNA virus, herpes simplex virus-1 (HSV-1) and a positive sense, single stranded RNA virus, Semliki Forest virus (SFV) to show that the BH3-only protein Puma is the major mediator of virus-induced Bax/Bak activation and ..
  50. Thaa B, Biasiotto R, Eng K, Neuvonen M, Götte B, Rheinemann L, et al. Differential Phosphatidylinositol-3-Kinase-Akt-mTOR Activation by Semliki Forest and Chikungunya Viruses Is Dependent on nsP3 and Connected to Replication Complex Internalization. J Virol. 2015;89:11420-37 pubmed publisher
    ..We report that this pathway was strongly activated in cells upon infection with the Old World alphavirus Semliki Forest virus (SFV), even under conditions of complete nutrient starvation...
  51. Ajbani S, Velhal S, Kadam R, Patel V, Bandivdekar A. Immunogenicity of Semliki Forest virus based self-amplifying RNA expressing Indian HIV-1C genes in mice. Int J Biol Macromol. 2015;81:794-802 pubmed publisher
    ..vaccines developed by individually cloning gag, env and polRT genes of primary HIV-1C Indian isolates using Semliki Forest virus (SFV) vector...
  52. Gambhira R, Keele B, Schell J, Hunter M, Dufour J, Montefiori D, et al. Transmitted/founder simian immunodeficiency virus envelope sequences in vesicular stomatitis and Semliki forest virus vector immunized rhesus macaques. PLoS ONE. 2014;9:e109678 pubmed publisher
    ..rhesus plasma in 3 groups of 6 immunized with vesicular stomatitis virus (VSV) vectors and boosted with Semliki forest virus (SFV) replicons expressing (a) SIVsmE660 gag-env (b) SIVsmE660 gag-env plus rhesus GM-CSF and (c) control ..
  53. Reynolds T, Buonocore L, Rose N, Rose J, Robek M. Virus-Like Vesicle-Based Therapeutic Vaccine Vectors for Chronic Hepatitis B Virus Infection. J Virol. 2015;89:10407-15 pubmed publisher
    ..Recently, an evolved, high-titer vaccine platform consisting of Semliki Forest virus RNA replicons that express the vesicular stomatitis virus glycoprotein (VSV G) has been described...
  54. Martikainen M, Niittykoski M, von und zu Fraunberg M, Immonen A, Koponen S, van Geenen M, et al. MicroRNA-Attenuated Clone of Virulent Semliki Forest Virus Overcomes Antiviral Type I Interferon in Resistant Mouse CT-2A Glioma. J Virol. 2015;89:10637-47 pubmed publisher
    ..We show here that the Semliki Forest virus (SFV) strain SFV4, which causes lethal encephalitis in mice, is able to infect and replicate independent of ..
  55. Wang Y, Yue X, Jin H, Liu G, Pan L, Wang G, et al. A suicidal DNA vaccine expressing the fusion protein of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in mice. J Virol Methods. 2015;225:35-40 pubmed publisher
    ..PPRV fusion (F) protein is important for the induction of immune responses against PPRV. We constructed a Semliki Forest virus (SFV) replicon-vectored DNA vaccine ("suicidal DNA vaccine") and evaluated its immunogenicity in BALB/c ..
  56. Quetglas J, Labiano S, Aznar M, Bolaños E, Azpilikueta A, Rodríguez I, et al. Virotherapy with a Semliki Forest Virus-Based Vector Encoding IL12 Synergizes with PD-1/PD-L1 Blockade. Cancer Immunol Res. 2015;3:449-54 pubmed publisher
    ..We have developed a cytolytic but nonreplicative viral vector system based on Semliki Forest virus that encodes IL12 (SFV-IL12)...
  57. Levanov L, Vera C, Vapalahti O. Prevalence estimation of tick-borne encephalitis virus (TBEV) antibodies in dogs from Finland using novel dog anti-TBEV IgG MAb-capture and IgG immunofluorescence assays based on recombinant TBEV subviral particles. Ticks Tick Borne Dis. 2016;7:979-982 pubmed publisher
    ..MAb)-capture assay which is based on TBEV prME subviral particles expressed in mammalian cells from Semliki Forest virus (SFV) replicon as well as IgG immunofluorescence assay (IFA) which is based on Vero E6 cells transfected ..
  58. Lemos M, Patiño S, Bernardino T, Coroadinha A, Soares H, Astray R, et al. Intracellular Delivery of HCV NS3p gene using vectored particles. J Biotechnol. 2018;274:33-39 pubmed publisher
    ..a tool to study this kind of presentation, using two vectored particle (VP) systems, one based on the Semliki Forest virus (SFV) and the other on HCV pseudoparticles (HCVpp), both carrying the protease domain of the NS3 gene...
  59. Carnero E, Barriocanal M, Prior C, Pablo Unfried J, Segura V, Guruceaga E, et al. Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication. EMBO Rep. 2016;17:1013-28 pubmed publisher
    ..EGOT EGOT is increased in the liver of patients infected with HCV and after infection with influenza or Semliki Forest virus (SFV)...
  60. Ng T, Cheung R, Wong J, Wang Y, Ip D, Wan D, et al. Antiviral activities of whey proteins. Appl Microbiol Biotechnol. 2015;99:6997-7008 pubmed publisher
    ..respiratory syncytial virus, hepatitis B virus, adenovirus, poliovirus, hantavirus, sindbis virus, semliki forest virus, echovirus, and enterovirus...
  61. Kramer M, Masner M, Casales E, Moreno M, Smerdou C, Chabalgoity J. Neoadjuvant administration of Semliki Forest virus expressing interleukin-12 combined with attenuated Salmonella eradicates breast cancer metastasis and achieves long-term survival in immunocompetent mice. BMC Cancer. 2015;15:620 pubmed publisher
    ..2×10(8) particles of a Semliki Forest virus vector expressing interleukin-12 (SFV-IL-12) and/or 2×10(7) units of an aroC (-) Samonella Typhimurium ..
  62. Lundstrom K. Semliki forest virus-based expression of recombinant GPCRs. Methods Enzymol. 2015;556:331-50 pubmed publisher
    ..Alphaviruses, particularly recombinant Semliki Forest virus (SFV) particles, have provided the means for expression of a number of GPCRs in a broad range of mammalian ..
  63. Panas M, Schulte T, Thaa B, Sandalova T, Kedersha N, Achour A, et al. Viral and cellular proteins containing FGDF motifs bind G3BP to block stress granule formation. PLoS Pathog. 2015;11:e1004659 pubmed publisher
    ..Many viruses, including Semliki Forest virus (SFV), block SG induction by targeting G3BP...
  64. Stiles K, Kielian M. Role of TSPAN9 in Alphavirus Entry and Early Endosomes. J Virol. 2016;90:4289-97 pubmed publisher
    ..identified TSPAN9 as a host factor that promotes infection by the alphaviruses Sindbis virus (SINV), Semliki Forest virus (SFV), and chikungunya virus (CHIKV)...
  65. Byler K, Collins J, Ogungbe I, Setzer W. Alphavirus protease inhibitors from natural sources: A homology modeling and molecular docking investigation. Comput Biol Chem. 2016;64:163-184 pubmed publisher
    ..proteases of CHIKV, ONNV, RRV, EEEV, VEEV, WEEV, as well as Aura virus (AURV), Barmah Forest Virus (BFV), Semliki Forest virus (SFV), and Sindbis virus (SINV) in order to identity structural scaffolds for inhibitor design or discovery...
  66. Waarts B, Smit J, Aneke O, McInerney G, Liljestrom P, Bittman R, et al. Reversible acid-induced inactivation of the membrane fusion protein of Semliki Forest virus. J Virol. 2005;79:7942-8 pubmed
    Previously, it has been shown that the exposure of Semliki Forest virus (SFV) to a mildly acidic environment induces a rapid and complete loss of the ability of the virus to bind and fuse to target membranes added subsequently...
  67. Ruiz Guillen M, Gabev E, Quetglas J, Casales E, Ballesteros Briones M, Poutou J, et al. Capsid-deficient alphaviruses generate propagative infectious microvesicles at the plasma membrane. Cell Mol Life Sci. 2016;73:3897-916 pubmed publisher
    ..In the present study, we show that Semliki Forest virus and Sindbis virus lacking the capsid gene can propagate in mammalian and insect cells...
  68. Xia S, Lei J, Du M, Wang Y, Cong X, Xiang G, et al. Enhanced protective immunity of the chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever in pigs by a Salmonella bacterial ghost adjuvant. Vet Res. 2016;47:64 pubmed publisher
    ..Previously, we demonstrated that rAdV-SFV-E2, an adenovirus-delivered, Semliki Forest virus replicon-vectored marker vaccine against CSF, is able to protect pigs against lethal CSFV challenge...
  69. Niittykoski M, von und zu Fraunberg M, Martikainen M, Rauramaa T, Immonen A, Koponen S, et al. Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Transl Oncol. 2017;10:772-779 pubmed publisher
    ..the infectivity of the oncolytic adenoviruses in interferon (IFN)-pretreated cells with IFN-sensitive Semliki Forest virus (SFV)...
  70. Xia S, Du M, Lei J, Liu Y, Wang Y, Ji S, et al. Piglets with maternally derived antibodies from sows immunized with rAdV-SFV-E2 were completely protected against lethal CSFV challenge. Vet Microbiol. 2016;190:38-42 pubmed publisher
    ..Previously, we have demonstrated that the DIVA vaccine rAdV-SFV-E2, an adenovirus-vectored Semliki Forest virus replicon expressing the E2 glycoprotein of CSFV, induces complete protection from lethal CSFV challenge...
  71. Varjak M, Maringer K, Watson M, Sreenu V, Fredericks A, Pondeville E, et al. Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses. mSphere. 2017;2: pubmed publisher
    ..of small RNAs captured by Piwi4 revealed that it is predominantly associated with virus-specific siRNAs in Semliki Forest virus-infected cells and, to a lesser extent, with viral piRNAs...
  72. Riedel H, Lehrach H, Garoff H. Nucleotide sequence at the junction between the nonstructural and the structural genes of the semliki forest virus genome. J Virol. 1982;42:725-9 pubmed
    The nucleotide sequence at the junction between the nonstructural and the structural genes of the Semliki Forest virus 42S RNA genome has been determined from cloned cDNA...
  73. Ferguson M, Saul S, Fragkoudis R, Weisheit S, Cox J, Patabendige A, et al. Ability of the Encephalitic Arbovirus Semliki Forest Virus To Cross the Blood-Brain Barrier Is Determined by the Charge of the E2 Glycoprotein. J Virol. 2015;89:7536-49 pubmed publisher
    b>Semliki Forest virus (SFV) provides a well-characterized model system to study the pathogenesis of virus encephalitis. Several studies have used virus derived from the molecular clone SFV4...
  74. Saul S, Ferguson M, Cordonin C, Fragkoudis R, Ool M, Tamberg N, et al. Differences in Processing Determinants of Nonstructural Polyprotein and in the Sequence of Nonstructural Protein 3 Affect Neurovirulence of Semliki Forest Virus. J Virol. 2015;89:11030-45 pubmed publisher
    The A7(74) strain of Semliki Forest virus (SFV; genus Alphavirus) is avirulent in adult mice, while the L10 strain is virulent in mice of all ages...
  75. Lundstrom K. Alphavirus-Based Vaccines. Methods Mol Biol. 2016;1404:313-28 pubmed publisher
    Alphavirus vectors based on Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus have been widely applied for vaccine development...
  76. Pingen M, Bryden S, Pondeville E, Schnettler E, Kohl A, Merits A, et al. Host Inflammatory Response to Mosquito Bites Enhances the Severity of Arbovirus Infection. Immunity. 2016;44:1455-69 pubmed publisher
    ..We show, using Semliki Forest virus and Bunyamwera virus, that these viruses use this inflammatory niche to aid their replication and ..
  77. Agopian A, Quetin M, Castano S. Structure and interaction with lipid membrane models of Semliki Forest virus fusion peptide. Biochim Biophys Acta. 2016;1858:2671-2680 pubmed publisher
    b>Semliki Forest virus (SFV) is a well-characterized alphavirus that infects cells via endocytosis and an acid-triggered fusion step using class II fusion proteins...
  78. Blome S, Moß C, Reimann I, König P, Beer M. Classical swine fever vaccines-State-of-the-art. Vet Microbiol. 2017;206:10-20 pubmed publisher
    ..At present, especially two new constructs have been intensively tested, the adenovirus-delivered, Semliki Forest virus replicon-vectored marker vaccine candidate "rAdV-SFV-E2" and the pestivirus chimera "CP7_E2alf"...
  79. Garoff H, Frischauf A, Simons K, Lehrach H, Delius H. The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region. Proc Natl Acad Sci U S A. 1980;77:6376-80 pubmed
    ..The amino acid sequence of the C protein shows a clustering of basic amino acids and prolines within the first 110 amino acids. ..
  80. Uchime O, Fields W, Kielian M. The role of E3 in pH protection during alphavirus assembly and exit. J Virol. 2013;87:10255-62 pubmed publisher
    ..tested the in vivo role of E3 in pH protection by alanine substitutions of E3 Y47 and Y48 (Y47/48A) in Semliki Forest virus. The mutant was nonviable and was blocked in E1 transport to the plasma membrane and virus production...
  81. Jia F, Miao H, Zhu X, Xu F. Pseudo-typed Semliki Forest virus delivers EGFP into neurons. J Neurovirol. 2017;23:205-215 pubmed publisher
    b>Semliki Forest virus (SFV), a neurotropic virus, has been used to deliver heterologous genes into cells in vitro and in vivo...
  82. Varghese F, Rausalu K, Hakanen M, Saul S, Kummerer B, Susi P, et al. Obatoclax Inhibits Alphavirus Membrane Fusion by Neutralizing the Acidic Environment of Endocytic Compartments. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..03 μM) and Semliki Forest virus (SFV; EC50 = 0.11 μM)...
  83. Pietilä M, van Hemert M, Ahola T. Purification of Highly Active Alphavirus Replication Complexes Demonstrates Altered Fractionation of Multiple Cellular Membranes. J Virol. 2018;92: pubmed publisher
    ..we established a protocol to purify these membranous replication complexes (RCs) from cells infected with Semliki Forest virus (SFV)...
  84. Weston S, Czieso S, White I, Smith S, Wash R, Diaz Soria C, et al. Alphavirus Restriction by IFITM Proteins. Traffic. 2016;17:997-1013 pubmed publisher
    ..We demonstrate that Sindbis and Semliki Forest virus (SFV), which both use endocytosis and acid-induced membrane fusion in early endosomes to infect cells, are ..
  85. Gullberg M, Lohse L, Bøtner A, McInerney G, Burman A, Jackson T, et al. A Prime-Boost Vaccination Strategy in Cattle to Prevent Foot-and-Mouth Disease Using a "Single-Cycle" Alphavirus Vector and Empty Capsid Particles. PLoS ONE. 2016;11:e0157435 pubmed publisher
    ..with the FMDV 3C protease (3Cpro) using a "single cycle" packaged alphavirus self-replicating RNA based on Semliki Forest virus (SFV)...
  86. Ramachandran M, Yu D, Dyczynski M, Baskaran S, Zhang L, Lulla A, et al. Safe and Effective Treatment of Experimental Neuroblastoma and Glioblastoma Using Systemically Delivered Triple MicroRNA-Detargeted Oncolytic Semliki Forest Virus. Clin Cancer Res. 2017;23:1519-1530 pubmed publisher
    ..Here we evaluate the oncolytic capacity of the neurovirulent and partly IFNβ-resistant Semliki Forest virus (SFV)-4 in glioblastoma multiformes and neuroblastomas...
  87. Kurena B, Vezane A, Skrastina D, Trofimova O, Zajakina A. Magnetic nanoparticles for efficient cell transduction with Semliki Forest virus. J Virol Methods. 2017;245:28-34 pubmed publisher
    b>Semliki Forest virus (SFV) is a potential cancer gene therapy vector capable of providing high and transient expression of heterologous proteins in mammalian cells...
  88. Lundstrom K. Alphavirus-Based Vaccines. Methods Mol Biol. 2017;1581:225-242 pubmed publisher
    Alphavirus-based vectors have been engineered from Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus and applied for vaccine development...
  89. Lundstrom K. Oncolytic Alphaviruses in Cancer Immunotherapy. Vaccines (Basel). 2017;5: pubmed publisher
    ..Moreover, animals were vaccinated with engineered oncolytic replication-deficient and -competent Semliki Forest virus, Sindbis virus and Venezuelan equine encephalitis virus vectors expressing various antigens...
  90. K ri inen L, Takkinen K, Ker nen S, S derlund H. Replication of the genome of alphaviruses. J Cell Sci Suppl. 1987;7:231-50 pubmed
    The genome of Semliki Forest virus (SFV) is 11,442 nucleotides with a 5' cap-structure and a 3' poly(A) tail of about 100 residues. The genome of the closely relate Sindbis virus (SIN) is slightly longer (11,703 nucleotides)...
  91. Umashankar M, S nchez San Mart n C, Liao M, Reilly B, Guo A, Taylor G, et al. Differential cholesterol binding by class II fusion proteins determines membrane fusion properties. J Virol. 2008;82:9245-53 pubmed publisher
    ..Using cholesterol-depleted insect cells, we showed that fusion and infection by the alphaviruses Semliki Forest virus (SFV) and Sindbis virus were strongly promoted by cholesterol, with similar sterol dependence in laboratory ..
  92. Sj berg M, Lindqvist B, Garoff H. Activation of the alphavirus spike protein is suppressed by bound E3. J Virol. 2011;85:5644-50 pubmed publisher
    ..In this work, we used a furin-resistant Semliki Forest virus (SFV) mutant, SFV(SQL), to study the role of E3 in spike activation...