Human enterovirus C

Summary

Alias: Poliovirus

Top Publications

  1. Bienz K, Egger D, Troxler M, Pasamontes L. Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region. J Virol. 1990;64:1156-63 pubmed
    Transcriptionally active replication complexes bound to smooth membrane vesicles were isolated from poliovirus-infected cells...
  2. Belov G, Habbersett C, Franco D, Ehrenfeld E. Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication. J Virol. 2007;81:9259-67 pubmed
    We have previously shown that synthesis of poliovirus protein 3CD in uninfected HeLa cell extracts induces an increased association with membranes of the cellular Arf GTPases, which are key players in cellular membrane traffic...
  3. Belov G, Fogg M, Ehrenfeld E. Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor. J Virol. 2005;79:7207-16 pubmed
    b>Poliovirus infection results in the disintegration of intracellular membrane structures and formation of specific vesicles that serve as sites for replication of viral RNA...
  4. Steil B, Barton D. Poliovirus cis-acting replication element-dependent VPg Uridylylation lowers the Km of the initiating nucleoside triphosphate for viral RNA replication. J Virol. 2008;82:9400-8 pubmed publisher
    ..By lowering the concentration of UTP needed for the initiation of RNA replication, CRE-dependent VPg uridylylation provides a mechanism for a more robust initiation of RNA replication. ..
  5. Kempf B, Kelly M, Springer C, Peersen O, Barton D. Structural features of a picornavirus polymerase involved in the polyadenylation of viral RNA. J Virol. 2013;87:5629-44 pubmed publisher
    ..Based on prior studies, we proposed a model wherein the poliovirus RNA-dependent RNA polymerase (3D(pol)) uses a reiterative transcription mechanism while replicating the poly(A) ..
  6. Shen M, Reitman Z, Zhao Y, Moustafa I, Wang Q, Arnold J, et al. Picornavirus genome replication. Identification of the surface of the poliovirus (PV) 3C dimer that interacts with PV 3Dpol during VPg uridylylation and construction of a structural model for the PV 3C2-3Dpol complex. J Biol Chem. 2008;283:875-88 pubmed
    ..This analysis identified numerous viable poliovirus mutants with reduced growth kinetics that correlated to reduced kinetics of RNA synthesis that was attributable ..
  7. Dorner A, Dorner L, Larsen G, Wimmer E, Anderson C. Identification of the initiation site of poliovirus polyprotein synthesis. J Virol. 1982;42:1017-28 pubmed
    The complete nucleotide sequence of poliovirus RNA has a long open reading frame capable of encoding the precursor polyprotein NCVP00...
  8. Springer C, Huntoon H, Peersen O. Polyprotein context regulates the activity of poliovirus 2CATPase bound to bilayer nanodiscs. J Virol. 2013;87:5994-6004 pubmed publisher
    Positive-strand RNA viruses generally replicate in large membrane-associated complexes. For poliovirus, these replication complexes are anchored to the membrane via the viral 2B, 2C, and 3A proteins...
  9. Roehl H, Parsley T, Ho T, Semler B. Processing of a cellular polypeptide by 3CD proteinase is required for poliovirus ribonucleoprotein complex formation. J Virol. 1997;71:578-85 pubmed
    b>Poliovirus interactions with host cells were investigated by studying the formation of ribonucleoprotein complexes at the 3' end of poliovirus negative-strand RNA which are presumed to be involved in viral RNA synthesis...

More Information

Publications71

  1. Liu J, Zhang H, Zhao Y, Xia L, Guo C, Yang H, et al. Characterization of four vaccine-related polioviruses including two intertypic type 3/type 2 recombinants associated with aseptic encephalitis. Virol J. 2016;13:162 pubmed
    ..The recombination of Sabin vaccine strains with other Sabin serotypes or human enterovirus C species could be a critical factor in the potential of emerging viruses and related disease outbreaks...
  2. Madan V, Redondo N, Carrasco L. Cell permeabilization by poliovirus 2B viroporin triggers bystander permeabilization in neighbouring cells through a mechanism involving gap junctions. Cell Microbiol. 2010;12:1144-57 pubmed publisher
    b>Poliovirus 2B protein is a well-known viroporin implicated in plasma membrane permeabilization to ions and low-molecular-weight compounds during infection...
  3. Kitamura N, Adler C, Rothberg P, Martinko J, Nathenson S, Wimmer E. The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies. Cell. 1980;21:295-302 pubmed
    The poliovirus genome-linked protein (VPg) has been subjected to radiochemical microsequence analysis...
  4. Bergelson J. New (fluorescent) light on poliovirus entry. Trends Microbiol. 2008;16:44-7 pubmed publisher
    To initiate infection, poliovirus must release its RNA genome into the cytoplasm of a target cell, a process called 'uncoating'. How this occurs has remained uncertain, despite studies over several decades...
  5. Wang J, Lyle J, Bullitt E. Surface for catalysis by poliovirus RNA-dependent RNA polymerase. J Mol Biol. 2013;425:2529-40 pubmed publisher
    The poliovirus RNA-dependent RNA polymerase, 3Dpol, replicates the viral genomic RNA on the surface of virus-induced intracellular membranes...
  6. Kitamura N, Wimmer E. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method. Proc Natl Acad Sci U S A. 1980;77:3196-200 pubmed
    ..amp; Coulson, A. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467] was modified to allow sequence analysis of poliovirus RNA without recourse to cloning...
  7. Jagdeo J, Dufour A, Fung G, Luo H, Kleifeld O, Overall C, et al. Heterogeneous Nuclear Ribonucleoprotein M Facilitates Enterovirus Infection. J Virol. 2015;89:7064-78 pubmed
    ..known as TAILS to identify protease-generated neo-N-terminal peptides, we identify a novel target of the poliovirus 3C proteinase, the heterogeneous nuclear ribonucleoproteinM(hnRNP M), a nucleocytoplasmic shuttling RNA-binding ..
  8. Lawson M, Semler B. Alternate poliovirus nonstructural protein processing cascades generated by primary sites of 3C proteinase cleavage. Virology. 1992;191:309-20 pubmed
    ..Both pulse-chase studies of infected cells and in vitro studies of the translation of poliovirus type 1 RNA transcribed from genomic cDNA clones indicate a specific cascade of polyprotein processing in which ..
  9. Kr usslich H, Nicklin M, Toyoda H, Etchison D, Wimmer E. Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220. J Virol. 1987;61:2711-8 pubmed
    b>Poliovirus infection of HeLa cells induces rapid shutoff of host protein synthesis, whereas translation of poliovirus RNA is not inhibited...
  10. Sharma R, Raychaudhuri S, Dasgupta A. Nuclear entry of poliovirus protease-polymerase precursor 3CD: implications for host cell transcription shut-off. Virology. 2004;320:195-205 pubmed publisher
    ..transcription mediated by all three RNA polymerases is rapidly inhibited after infection of mammalian cells with poliovirus (PV)...
  11. Pathak H, Ghosh S, Roberts A, Sharma S, Yoder J, Arnold J, et al. Structure-function relationships of the RNA-dependent RNA polymerase from poliovirus (3Dpol). A surface of the primary oligomerization domain functions in capsid precursor processing and VPg uridylylation. J Biol Chem. 2002;277:31551-62 pubmed publisher
    The primary oligomerization domain of poliovirus polymerase, 3Dpol, is stabilized by the interaction of the back of the thumb subdomain of one molecule with the back of the palm subdomain of a second molecule, thus permitting the head-to-..
  12. Marcotte L, Wass A, Gohara D, Pathak H, Arnold J, Filman D, et al. Crystal structure of poliovirus 3CD protein: virally encoded protease and precursor to the RNA-dependent RNA polymerase. J Virol. 2007;81:3583-96 pubmed publisher
    b>Poliovirus 3CD is a multifunctional protein that serves as a precursor to the protease 3C(pro) and the viral polymerase 3D(pol) and also plays a role in the control of viral replication...
  13. Strauss D, Wuttke D. Characterization of protein-protein interactions critical for poliovirus replication: analysis of 3AB and VPg binding to the RNA-dependent RNA polymerase. J Virol. 2007;81:6369-78 pubmed publisher
    Two critical interactions within the poliovirus RNA replication complex are those of the RNA-dependent RNA polymerase 3D with the viral proteins 3AB and VPg...
  14. Ogram S, Spear A, Sharma N, Flanegan J. The 5'CL-PCBP RNP complex, 3' poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA. Virology. 2010;397:14-22 pubmed publisher
    ..These results are consistent with a model in which the 5'CL-PCBP complex interacts with the 3' poly(A)-PABP complex to form a 5'-3' circular complex that facilitates ribosome reloading and stimulates PV RNA translation...
  15. Cho M, Teterina N, Egger D, Bienz K, Ehrenfeld E. Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells. Virology. 1994;202:129-45 pubmed publisher
    b>Poliovirus (PV)-infected cells undergo extensive proliferation and rearrangement of intracellular smooth membranes to generate vesicles on which viral RNA replication occurs...
  16. Lama J, Sanz M, Rodr guez P. A role for 3AB protein in poliovirus genome replication. J Biol Chem. 1995;270:14430-8 pubmed
    The poliovirus polypeptide 3AB, the precursor of the genome-bound VPg protein, stimulates in vitro the synthesis of poly(U) directed by the viral polymerase 3Dpol (Lama, J., Paul, A., Harris, K., and Wimmer, E. (1994) J. Biol. Chem...
  17. Bonderoff J, Larey J, Lloyd R. Cleavage of poly(A)-binding protein by poliovirus 3C proteinase inhibits viral internal ribosome entry site-mediated translation. J Virol. 2008;82:9389-99 pubmed publisher
    ..Here we show that 3C(pro) inhibits cap-independent translation mediated by the poliovirus internal ribosome entry site (IRES) in a dose-dependent manner in HeLa translation extracts displaying cap-poly(..
  18. Kitamura N, Semler B, Rothberg P, Larsen G, Adler C, Dorner A, et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981;291:547-53 pubmed
    The primary structure of the poliovirus genome has been determined. The RNA molecule is 7,433 nucleotides long, polyadenylated at the 3' terminus, and covalently linked to a small protein (VPg) at the 5' terminus...
  19. Oh H, Pathak H, Goodfellow I, Arnold J, Cameron C. Insight into poliovirus genome replication and encapsidation obtained from studies of 3B-3C cleavage site mutants. J Virol. 2009;83:9370-87 pubmed publisher
    A poliovirus (PV) mutant (termed GG), which is incapable of producing 3AB, VPg, and 3CD proteins due to a defective cleavage site between the 3B and 3C proteins, replicated, producing 3BC-linked RNA rather than the VPg-linked RNA produced ..
  20. Adams P, Kandiah E, Effantin G, Steven A, Ehrenfeld E. Poliovirus 2C protein forms homo-oligomeric structures required for ATPase activity. J Biol Chem. 2009;284:22012-21 pubmed publisher
    The poliovirus protein 2C plays an essential role in viral RNA replication, although its precise biochemical activities or structural requirements have not been elucidated...
  21. Pathak H, Oh H, Goodfellow I, Arnold J, Cameron C. Picornavirus genome replication: roles of precursor proteins and rate-limiting steps in oriI-dependent VPg uridylylation. J Biol Chem. 2008;283:30677-88 pubmed publisher
    ..Finally, determinants of VPg uridylylation efficiency suggest formation and/or collapse or release of the uridylylated product as the rate-limiting step in vitro depending upon the VPg donor employed...
  22. Liu Y, Franco D, Paul A, Wimmer E. Tyrosine 3 of poliovirus terminal peptide VPg(3B) has an essential function in RNA replication in the context of its precursor protein, 3AB. J Virol. 2007;81:5669-84 pubmed publisher
    b>Poliovirus (PV) VPg is a genome-linked protein that is essential for the initiation of viral RNA replication...
  23. Jurgens C, Barton D, Sharma N, Morasco B, Ogram S, Flanegan J. 2Apro is a multifunctional protein that regulates the stability, translation and replication of poliovirus RNA. Virology. 2006;345:346-57 pubmed publisher
    b>Poliovirus 2A(pro) is required for the inhibition of host cell protein synthesis and efficient viral replication. We investigated the role of 2A(pro) in regulating viral RNA stability, translation and replication in HeLa S10 reactions...
  24. Ypma Wong M, Dewalt P, Johnson V, Lamb J, Semler B. Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursor. Virology. 1988;166:265-70 pubmed
    ..It is, therefore, important to determine the enzymes responsible for each proteolytic event. The poliovirus protein 3C has been identified as a proteinase which specifically cleaves between Q-G pairs...
  25. Igarashi H, Yoshino Y, Miyazawa M, Horie H, Ohka S, Nomoto A. 2A protease is not a prerequisite for poliovirus replication. J Virol. 2010;84:5947-57 pubmed publisher
    b>Poliovirus (PV) 2A(pro) has been considered important for PV replication and is known to be toxic to host cells. A 2A(pro)-deficient PV would potentially be less toxic and ideal as a vector...
  26. Arnold J, Vignuzzi M, Stone J, Andino R, Cameron C. Remote site control of an active site fidelity checkpoint in a viral RNA-dependent RNA polymerase. J Biol Chem. 2005;280:25706-16 pubmed publisher
    ..we show that a conformational change step preceding phosphoryl transfer is a key fidelity checkpoint for the poliovirus RdRp (3Dpol)...
  27. Franco D, Pathak H, Cameron C, Rombaut B, Wimmer E, Paul A. Stimulation of poliovirus synthesis in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro. J Virol. 2005;79:6358-67 pubmed publisher
    The plus-strand RNA genome of poliovirus serves three distinct functions in the life cycle of the virus. The RNA is translated and then replicated, and finally the progeny RNAs are encapsidated...
  28. Schein C, Oezguen N, van der Heden van Noort G, Filippov D, Paul A, Kumar E, et al. NMR solution structure of poliovirus uridylyated peptide linked to the genome (VPgpU). Peptides. 2010;31:1441-8 pubmed publisher
    ..This first complete structure for any uridylylated VPg, of poliovirus type 1 (PV1)-VPgpU, shows that conserved amino acids in VPg stabilize the bound UMP, with the uridine atoms ..
  29. Gamarnik A, Andino R. Interactions of viral protein 3CD and poly(rC) binding protein with the 5' untranslated region of the poliovirus genome. J Virol. 2000;74:2219-26 pubmed
    The poly(rC) binding protein (PCBP) is a cellular protein required for poliovirus replication...
  30. Mart nez Gil L, Ba Polo M, Redondo N, S nchez Mart nez S, Nieva J, Carrasco L, et al. Membrane integration of poliovirus 2B viroporin. J Virol. 2011;85:11315-24 pubmed publisher
    ..In addition, the in vitro translation of several truncated versions of the 2B protein suggests that the two hydrophobic regions cooperate to insert into the ER-derived microsomal membranes...
  31. Harris K, Reddigari S, Nicklin M, H mmerle T, Wimmer E. Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase. J Virol. 1992;66:7481-9 pubmed
    A cDNA clone encoding the 3CD proteinase (3CDpro) of poliovirus type 2 (Sabin), the precursor to proteinase 3Cpro and RNA polymerase 3Dpol, was expressed in bacteria by using a T7 expression system...
  32. Pilipenko E, Poperechny K, Maslova S, Melchers W, Slot H, Agol V. Cis-element, oriR, involved in the initiation of (-) strand poliovirus RNA: a quasi-globular multi-domain RNA structure maintained by tertiary ('kissing') interactions. EMBO J. 1996;15:5428-36 pubmed
    The key steps in the replication of the poliovirus genome, initiation of (-) and (+) strands, require two different cis-acting elements, oriR and oriL, respectively...
  33. Aldabe R, Barco A, Carrasco L. Membrane permeabilization by poliovirus proteins 2B and 2BC. J Biol Chem. 1996;271:23134-7 pubmed
    b>Poliovirus infection leads to drastic alterations in membrane permeability late during infection...
  34. Barco A, Carrasco L. A human virus protein, poliovirus protein 2BC, induces membrane proliferation and blocks the exocytic pathway in the yeast Saccharomyces cerevisiae. EMBO J. 1995;14:3349-64 pubmed
    Inducible synthesis of poliovirus protein 2BC in Saccharomyces cerevisiae arrests cell growth in the G2 phase of the cell cycle, while no effects are observed upon expression of poliovirus genes 2B or 2C, either individually or in ..
  35. Hobdey S, Kempf B, Steil B, Barton D, Peersen O. Poliovirus polymerase residue 5 plays a critical role in elongation complex stability. J Virol. 2010;84:8072-84 pubmed publisher
    ..In this work, we extend our studies to show that poliovirus polymerase activity is also dependent on the nature of residue 5 and further elucidate which aspects of ..
  36. Pelletier J, Sonenberg N. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature. 1988;334:320-5 pubmed publisher
    b>Poliovirus RNA is naturally uncapped, therefore its translation must proceed via a cap-independent mechanism. Translation initiation on poliovirus RNA occurs by binding of ribosomes to an internal sequence within the 5' noncoding region...
  37. Lin J, Cheng N, Chow M, Filman D, Steven A, Hogle J, et al. An externalized polypeptide partitions between two distinct sites on genome-released poliovirus particles. J Virol. 2011;85:9974-83 pubmed publisher
    During cell entry, native poliovirus (160S) converts to a cell-entry intermediate (135S) particle, resulting in the externalization of capsid proteins VP4 and the amino terminus of VP1 (residues 1 to 53)...
  38. Wang C, Jiang P, Sand C, Paul A, Wimmer E. Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis. J Virol. 2012;86:9964-75 pubmed publisher
    Polypeptide 2C(ATPase) is one of the most thoroughly studied but least understood proteins in the life cycle of poliovirus. Within the protein, multiple functional domains important for uncoating, host cell membrane alterations, and RNA ..
  39. Yang X, Smidansky E, Maksimchuk K, Lum D, Welch J, Arnold J, et al. Motif D of viral RNA-dependent RNA polymerases determines efficiency and fidelity of nucleotide addition. Structure. 2012;20:1519-27 pubmed publisher
    ..We conclude that motif D of RdRps and, by inference, RTs is the functional equivalent to the fidelity helix of other polymerases...
  40. Pilipenko E, Maslova S, Sinyakov A, Agol V. Towards identification of cis-acting elements involved in the replication of enterovirus and rhinovirus RNAs: a proposal for the existence of tRNA-like terminal structures. Nucleic Acids Res. 1992;20:1739-45 pubmed
    ..The two latter models were verified for poliovirus and coxsackievirus B genomes by testing with single-strand and double-strand specific enzymatic and chemical ..
  41. Rivera C, Lloyd R. Modulation of enteroviral proteinase cleavage of poly(A)-binding protein (PABP) by conformation and PABP-associated factors. Virology. 2008;375:59-72 pubmed publisher
    b>Poliovirus (PV) causes a drastic inhibition of cellular cap-dependant protein synthesis due to the cleavage of translation factors eukaryotic initiation factor 4G (eIF4G) and poly(A) binding protein (PABP)...
  42. Racaniello V, Baltimore D. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc Natl Acad Sci U S A. 1981;78:4887-91 pubmed
    The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA...
  43. Emini E, Elzinga M, Wimmer E. Carboxy-terminal analysis of poliovirus proteins: termination of poliovirus RNA translation and location of unique poliovirus polyprotein cleavage sites. J Virol. 1982;42:194-9 pubmed
    The carboxy-terminal amino acids of a number of poliovirus proteins were determined by carboxypeptidase A analysis. The nonstructural proteins P3-2, P3-4b and their precursor...
  44. Etchison D, Milburn S, Edery I, Sonenberg N, Hershey J. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex. J Biol Chem. 1982;257:14806-10 pubmed
    Following poliovirus infection of HeLa cells, the synthesis of cellular proteins is inhibited but translation of poliovirus mRNA proceeds...
  45. Teterina N, Gorbalenya A, Egger D, Bienz K, Ehrenfeld E. Poliovirus 2C protein determinants of membrane binding and rearrangements in mammalian cells. J Virol. 1997;71:8962-72 pubmed
    b>Poliovirus protein 2C is a 329-amino acid-protein that is essential for viral RNA synthesis and may perform multiple functions. In infected cells, it is associated with virus-specific membrane vesicles...
  46. Gamarnik A, Andino R. Switch from translation to RNA replication in a positive-stranded RNA virus. Genes Dev. 1998;12:2293-304 pubmed
    ..In positive-stranded viruses, the genomic RNA serves as a template for both translation and RNA replication. Using poliovirus as a model, we examined the interaction between these two processes...
  47. Goldstaub D, Gradi A, Bercovitch Z, Grosmann Z, Nophar Y, Luria S, et al. Poliovirus 2A protease induces apoptotic cell death. Mol Cell Biol. 2000;20:1271-7 pubmed
    A cell line was generated that expresses the poliovirus 2A protease in an inducible manner. Tightly controlled expression was achieved by utilizing the muristerone A-regulated expression system...
  48. Back S, Kim Y, Kim W, Cho S, Oh H, Kim J, et al. Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro). J Virol. 2002;76:2529-42 pubmed
    ..such as polypyrimidine tract-binding protein (PTB) and poly(rC)-binding protein (PCBP), are required for the poliovirus IRES-dependent translation...
  49. Brunner J, Nguyen J, Roehl H, Ho T, Swiderek K, Semler B. Functional interaction of heterogeneous nuclear ribonucleoprotein C with poliovirus RNA synthesis initiation complexes. J Virol. 2005;79:3254-66 pubmed publisher
    We had previously demonstrated that a cellular protein specifically interacts with the 3' end of poliovirus negative-strand RNA. We now report the identity of this protein as heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2...
  50. Bubeck D, Filman D, Cheng N, Steven A, Hogle J, Belnap D. The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes. J Virol. 2005;79:7745-55 pubmed publisher
    b>Poliovirus provides a well-characterized system for understanding how nonenveloped viruses enter and infect cells. Upon binding its receptor, poliovirus undergoes an irreversible conformational change to the 135S cell entry intermediate...
  51. Destefano J, Titilope O. Poliovirus protein 3AB displays nucleic acid chaperone and helix-destabilizing activities. J Virol. 2006;80:1662-71 pubmed publisher
    b>Poliovirus protein 3AB displayed nucleic acid chaperone activity in promoting the hybridization of complementary nucleic acids and destabilizing secondary structure...
  52. Toyoda H, Nicklin M, Murray M, Anderson C, Dunn J, Studier F, et al. A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell. 1986;45:761-70 pubmed
    The poliovirus polyprotein is cleaved at three different amino acid pairs. Viral polypeptide 3C is responsible for processing at the most common pair (glutamineglycine)...
  53. Hogle J, Chow M, Filman D. Three-dimensional structure of poliovirus at 2.9 A resolution. Science. 1985;229:1358-65 pubmed
    The three-dimensional structure of poliovirus has been determined at 2.9 A resolution by x-ray crystallographic methods...
  54. de Breyne S, Bonderoff J, Chumakov K, Lloyd R, Hellen C. Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases. Virology. 2008;378:118-22 pubmed publisher
    The enteroviruses poliovirus (PV), Coxsackie B virus (CVB) and rhinovirus (HRV) are members of Picornaviridae that inhibit host cell translation early in infection...
  55. Yang Y, Rijnbrand R, Watowich S, Lemon S. Genetic evidence for an interaction between a picornaviral cis-acting RNA replication element and 3CD protein. J Biol Chem. 2004;279:12659-67 pubmed publisher
    ..cres, are essential for replication of the genomes of picornaviruses such as human rhinovirus 14 (HRV-14) and poliovirus because they template uridylylation of the protein primer, VPg, by the polymerase 3D(pol)...
  56. Yang X, Welch J, Arnold J, Boehr D. Long-range interaction networks in the function and fidelity of poliovirus RNA-dependent RNA polymerase studied by nuclear magnetic resonance. Biochemistry. 2010;49:9361-71 pubmed publisher
    The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus...
  57. Teterina N, Pinto Y, Weaver J, Jensen K, Ehrenfeld E. Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells. J Virol. 2011;85:4284-96 pubmed publisher
    b>Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function...
  58. Ventoso I, MacMillan S, Hershey J, Carrasco L. Poliovirus 2A proteinase cleaves directly the eIF-4G subunit of eIF-4F complex. FEBS Lett. 1998;435:79-83 pubmed
    ..mode of eIF-4G cleavage through the activation of a second cellular proteinase has been proposed in the case of poliovirus. Although cleavage of eIF4G by rhino- and coxsackievirus 2Apro has been achieved directly in vitro, a similar ..
  59. Barco A, Carrasco L. Identification of regions of poliovirus 2BC protein that are involved in cytotoxicity. J Virol. 1998;72:3560-70 pubmed
    The expression of poliovirus 2BC protein in yeast and mammalian cells leads to a number of metabolic and morphological alterations, such as growth inhibition, intracellular membrane proliferation, blockade of the exocytic pathway, and ..
  60. Amero C, Arnold J, Moustafa I, Cameron C, Foster M. Identification of the oriI-binding site of poliovirus 3C protein by nuclear magnetic resonance spectroscopy. J Virol. 2008;82:4363-70 pubmed publisher
    ..We have studied the poliovirus 3C-oriI interaction in order to begin to decipher the code of RNA recognition by picornaviral 3C proteins...
  61. Belov G, Feng Q, Nikovics K, Jackson C, Ehrenfeld E. A critical role of a cellular membrane traffic protein in poliovirus RNA replication. PLoS Pathog. 2008;4:e1000216 pubmed publisher
    Replication of many RNA viruses is accompanied by extensive remodeling of intracellular membranes. In poliovirus-infected cells, ER and Golgi stacks disappear, while new clusters of vesicle-like structures form sites for viral RNA ..
  62. Kempf B, Peersen O, Barton D. Poliovirus Polymerase Leu420 Facilitates RNA Recombination and Ribavirin Resistance. J Virol. 2016;90:8410-21 pubmed publisher
    ..In this study, we examined the structure and function of poliovirus polymerase, 3D(pol), as it relates to RNA recombination...