Enterobacteria phage T4

Summary

Alias: Bacteriophage T4, T4, coliphage T4, phage T4

Top Publications

  1. Tiemann B, Depping R, Gineikiene E, Kaliniene L, Nivinskas R, R ger W. ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: catalytic properties and mutation analysis. J Bacteriol. 2004;186:7262-72 pubmed publisher
    b>Bacteriophage T4 encodes three ADP-ribosyltransferases, Alt, ModA, and ModB. These enzymes participate in the regulation of the T4 replication cycle by ADP-ribosylating a defined set of host proteins...
  2. Bullard D, Bowater R. Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4. Biochem J. 2006;398:135-44 pubmed publisher
    The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria...
  3. Fokine A, Zhang Z, Kanamaru S, Bowman V, Aksyuk A, Arisaka F, et al. The molecular architecture of the bacteriophage T4 neck. J Mol Biol. 2013;425:1731-44 pubmed publisher
    A hexamer of the bacteriophage T4 tail terminator protein, gp15, attaches to the top of the phage tail stabilizing the contractile sheath and forming the interface for binding of the independently assembled head...
  4. Clare D, Bakkes P, van Heerikhuizen H, van der Vies S, Saibil H. Chaperonin complex with a newly folded protein encapsulated in the folding chamber. Nature. 2009;457:107-10 pubmed publisher
    ..The bacteriophage T4 requires its own version of GroES, gp31, which forms a taller folding chamber, to fold the major viral capsid ..
  5. De la Rosa M, Nelson S. An interaction between the Walker A and D-loop motifs is critical to ATP hydrolysis and cooperativity in bacteriophage T4 Rad50. J Biol Chem. 2011;286:26258-66 pubmed publisher
    ..Here, we evaluate the functional role of the D-loop using a bacteriophage T4 ABC protein, Rad50 (gp46)...
  6. Kanamaru S, Ishiwata Y, Suzuki T, Rossmann M, Arisaka F. Control of bacteriophage T4 tail lysozyme activity during the infection process. J Mol Biol. 2005;346:1013-20 pubmed publisher
    b>Bacteriophage T4 has an efficient mechanism for injecting the host Escherichiacoli cell with genomic DNA...
  7. Twist K, Campbell E, Deighan P, Nechaev S, Jain V, Geiduschek E, et al. Crystal structure of the bacteriophage T4 late-transcription coactivator gp33 with the ?-subunit flap domain of Escherichia coli RNA polymerase. Proc Natl Acad Sci U S A. 2011;108:19961-6 pubmed publisher
    Activated transcription of the bacteriophage T4 late genes, which is coupled to concurrent DNA replication, is accomplished by an initiation complex containing the host RNA polymerase associated with two phage-encoded proteins, gp55 (the ..
  8. Dudas K, Kreuzer K. Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. J Biol Chem. 2005;280:21561-9 pubmed publisher
    b>Bacteriophage T4 initiates origin-dependent replication via an R-loop mechanism in vivo...
  9. Farb J, Morrical S. Role of allosteric switch residue histidine 195 in maintaining active-site asymmetry in presynaptic filaments of bacteriophage T4 UvsX recombinase. J Mol Biol. 2009;385:393-404 pubmed publisher
    ..The T4 UvsX protein is a divergent RecA ortholog and contains histidine (His195) in place of glutamine at the allosteric ..

More Information

Publications111 found, 100 shown here

  1. Dixit A, Ray K, Thomas J, Black L. The C-terminal domain of the bacteriophage T4 terminase docks on the prohead portal clip region during DNA packaging. Virology. 2013;446:293-302 pubmed publisher
    ..docking interactions at specifically localized residues within a terminase-interaction region (aa279-316) in the phage T4 portal protein gp20 equated to the clip domain of the SPP1 portal crystal structure by 3D modeling...
  2. Gruidl M, Chen T, Gargano S, Storlazzi A, Cascino A, Mosig G. Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair gene uvsY belong to spatially and temporally overlapping transcription units. Virology. 1991;184:359-69 pubmed
    The bacteriophage T4 DNA recombination-repair gene uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26...
  3. Powell D, Franklin J, Arisaka F, Mosig G. Bacteriophage T4 DNA packaging genes 16 and 17. Nucleic Acids Res. 1990;18:4005 pubmed
  4. Durand S, Richard G, Bontems F, Uzan M. Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs. Proc Natl Acad Sci U S A. 2012;109:7073-8 pubmed publisher
    The bacteriophage T4-encoded RegB endoribonuclease is produced during the early stage of phage development and targets mostly (but not exclusively) the Shine-Dalgarno sequences of early genes...
  5. Kadyrov F, Drake J. UvsX recombinase and Dda helicase rescue stalled bacteriophage T4 DNA replication forks in vitro. J Biol Chem. 2004;279:35735-40 pubmed publisher
    ..b>Bacteriophage T4 encodes almost all of the proteins required for its own DNA replication, recombination, and repair...
  6. Snyder L, Tarkowski H. The N terminus of the head protein of T4 bacteriophage directs proteins to the GroEL chaperonin. J Mol Biol. 2005;345:375-86 pubmed
    The head protein of T4 bacteriophage requires the GroEL chaperonin for its insertion into a growing T4 head...
  7. Mosig G, Lin G, Franklin J, Fan W. Functional relationships and structural determinants of two bacteriophage T4 lysozymes: a soluble (gene e) and a baseplate-associated (gene 5) protein. New Biol. 1989;1:171-9 pubmed
    ..In bacteriophage T4, the major soluble lysozyme is the product of the e gene, gpe (gene product = gp)...
  8. Marusich E, Mesyanzhinov V. Nucleotide and deduced amino acid sequences of bacteriophage T4 gene 20. Nucleic Acids Res. 1989;17:7514 pubmed
  9. V lker T, Gafner J, Bickle T, Showe M. Gene 67, a new, essential bacteriophage T4 head gene codes for a prehead core component, PIP. I. Genetic mapping and DNA sequence. J Mol Biol. 1982;161:479-89 pubmed
  10. Kadyrov F, Shlyapnikov M, Kryukov V. A phage T4 site-specific endonuclease, SegE, is responsible for a non-reciprocal genetic exchange between T-even-related phages. FEBS Lett. 1997;415:75-80 pubmed
    The bacteriophage T4 segE gene encoding site-specific endonuclease lies between the hoc.1 and uvsW genes. The similar region of T-even-related phage RB30 lacks the segE gene...
  11. Vaiskunaite R, Miller A, Davenport L, Mosig G. Two new early bacteriophage T4 genes, repEA and repEB, that are important for DNA replication initiated from origin E. J Bacteriol. 1999;181:7115-25 pubmed
    Two new, small, early bacteriophage T4 genes, repEA and repEB, located within the origin E (oriE) region of T4 DNA replication, affect functioning of this origin...
  12. Shcherbakov V, Granovsky I, Plugina L, Shcherbakova T, Sizova S, Pyatkov K, et al. Focused genetic recombination of bacteriophage t4 initiated by double-strand breaks. Genetics. 2002;162:543-56 pubmed
    ..double-strand-break (DSB)-induced genetic recombination in vivo based on the ets1 segCDelta strain of bacteriophage T4 was developed...
  13. Pineda M, Gregory B, Szczypinski B, Baxter K, Hochschild A, Miller E, et al. A family of anti-sigma70 proteins in T4-type phages and bacteria that are similar to AsiA, a Transcription inhibitor and co-activator of bacteriophage T4. J Mol Biol. 2004;344:1183-97 pubmed publisher
    ..The bacteriophage T4 anti-sigma70 protein, AsiA, binds tightly to regions 4.1 and 4...
  14. Nechaev S, Kamali Moghaddam M, Andr E, L onetti J, Geiduschek E. The bacteriophage T4 late-transcription coactivator gp33 binds the flap domain of Escherichia coli RNA polymerase. Proc Natl Acad Sci U S A. 2004;101:17365-70 pubmed publisher
    Transcription of bacteriophage T4 late genes requires concomitant DNA replication...
  15. Hinton D, Pande S, Wais N, Johnson X, Vuthoori M, Makela A, et al. Transcriptional takeover by sigma appropriation: remodelling of the sigma70 subunit of Escherichia coli RNA polymerase by the bacteriophage T4 activator MotA and co-activator AsiA. Microbiology. 2005;151:1729-40 pubmed publisher
    Activation of bacteriophage T4 middle promoters, which occurs about 1 min after infection, uses two phage-encoded factors that change the promoter specificity of the host RNA polymerase...
  16. Kondabagil K, Rao V. A critical coiled coil motif in the small terminase, gp16, from bacteriophage T4: insights into DNA packaging initiation and assembly of packaging motor. J Mol Biol. 2006;358:67-82 pubmed publisher
    ..The phage T4 motor is composed of the small terminase protein, gpl6 (18kDa), the large terminase protein, gp17 (70kDa), and ..
  17. Fokine A, Battisti A, Kostyuchenko V, Black L, Rossmann M. Cryo-EM structure of a bacteriophage T4 gp24 bypass mutant: the evolution of pentameric vertex proteins in icosahedral viruses. J Struct Biol. 2006;154:255-9 pubmed publisher
    ..Nevertheless, deletion of the special vertex protein gene product 24 (gp24) in bacteriophage T4 can be compensated by mutations in the homologous major capsid protein gp23...
  18. Liu J, Bond J, Morrical S. Mechanism of presynaptic filament stabilization by the bacteriophage T4 UvsY recombination mediator protein. Biochemistry. 2006;45:5493-502 pubmed
    UvsY is the recombination mediator protein (RMP) of bacteriophage T4, which promotes homologous recombination by facilitating presynaptic filament assembly...
  19. Nelson S, Benkovic S. The T4 phage UvsW protein contains both DNA unwinding and strand annealing activities. J Biol Chem. 2007;282:407-16 pubmed
    UvsW protein belongs to the SF2 helicase family and is one of three helicases found in T4 phage...
  20. Long D, Kreuzer K. Fork regression is an active helicase-driven pathway in bacteriophage T4. EMBO Rep. 2009;10:394-9 pubmed publisher
    ..To investigate the biological role of fork regression in bacteriophage T4, we tested several mechanistic models of regression: strand exchange-mediated extrusion, topology-driven fork ..
  21. Yuan A, Hochschild A. Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression. Mol Microbiol. 2009;74:1018-30 pubmed publisher
    The bacteriophage T4 AsiA protein is a bifunctional regulator that inhibits transcription from the major class of bacterial promoters and also serves as an essential co-activator of transcription from T4 middle promoters...
  22. Andersson C, Lagerb ck P, Carlson K. Structure of bacteriophage T4 endonuclease II mutant E118A, a tetrameric GIY-YIG enzyme. J Mol Biol. 2010;397:1003-16 pubmed publisher
    b>Coliphage T4 endonuclease II (EndoII), encoded by gene denA, is a small (16 kDa, 136 aa) enzyme belonging to the GIY-YIG family of endonucleases, which lacks a C-terminal domain corresponding to that providing most of the binding energy ..
  23. Golec P, Wiczk A, Majchrzyk A, o J, W grzyn G, o M. A role for accessory genes rI.-1 and rI.1 in the regulation of lysis inhibition by bacteriophage T4. Virus Genes. 2010;41:459-68 pubmed publisher
    ..In this report, we demonstrate the results of genetic experiments which suggest a synergistic action of two accessory proteins of bacteriophage T4, RI.-1, and RI.1 with RIII in the regulation of LIN.
  24. Otsuka Y, Yonesaki T. Dmd of bacteriophage T4 functions as an antitoxin against Escherichia coli LsoA and RnlA toxins. Mol Microbiol. 2012;83:669-81 pubmed
    ..Interestingly, T4 phage Dmd suppressed the toxicities of both RnlA and LsoA by direct interaction, the first example of a phage with ..
  25. Perumal S, Nelson S, Benkovic S. Interaction of T4 UvsW helicase and single-stranded DNA binding protein gp32 through its carboxy-terminal acidic tail. J Mol Biol. 2013;425:2823-39 pubmed publisher
    b>Bacteriophage T4 UvsW helicase contains both unwinding and annealing activities and displays some functional similarities to bacterial RecG and RecQ helicases...
  26. Bova R, Cascino A, Cipollaro M, Grau O, Micheli M, Santoro M, et al. Bacteriophage T4 gene 27. Nucleic Acids Res. 1990;18:3046 pubmed
  27. Carles Kinch K, George J, Kreuzer K. Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins. EMBO J. 1997;16:4142-51 pubmed
    b>Bacteriophage T4 UvsW protein is involved in phage recombination, repair and the regulation of replication origins. Here, we provide evidence that UvsW functions as a helicase...
  28. Franklin J, Haseltine D, Davenport L, Mosig G. The largest (70 kDa) product of the bacteriophage T4 DNA terminase gene 17 binds to single-stranded DNA segments and digests them towards junctions with double-stranded DNA. J Mol Biol. 1998;277:541-57 pubmed publisher
    ..Most terminases are encoded by two partially overlapping genes. In phage T4 they are genes 16 and 17...
  29. Tran T, Struck D, Young R. Periplasmic domains define holin-antiholin interactions in t4 lysis inhibition. J Bacteriol. 2005;187:6631-40 pubmed
    b>Bacteriophage T4 effects host lysis with a holin, T, and an endolysin, E. T and E accumulate in the membrane and cytoplasm, respectively, throughout the period of late gene expression...
  30. Byrd A, Raney K. Increasing the length of the single-stranded overhang enhances unwinding of duplex DNA by bacteriophage T4 Dda helicase. Biochemistry. 2005;44:12990-7 pubmed
    ..Rather, multiple molecules of Dda bound to the same substrate exhibit greater processivity for DNA unwinding. ..
  31. Nelson S, Perumal S, Benkovic S. Processive and unidirectional translocation of monomeric UvsW helicase on single-stranded DNA. Biochemistry. 2009;48:1036-46 pubmed publisher
    UvsW protein from bacteriophage T4 controls the transition from origin-dependent to origin-independent initiation of replication through the unwinding of R-loops bound to the T4 origins of replication...
  32. Li V, Hogg M, Reha Krantz L. Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase. J Mol Biol. 2010;400:295-308 pubmed publisher
    ..The importance of the motif for function of the bacteriophage T4 DNA polymerase was revealed by suppressor analysis...
  33. Casjens S, Jacobs Sera D, Hatfull G, Hendrix R. Genome Sequence of Salmonella enterica Phage Det7. Genome Announc. 2015;3: pubmed publisher
    ..We report here the complete 157,498-bp sequence of its genome. Det7, together with its Vi01-like relatives, are distantly related to phage T4.
  34. Jose D, Weitzel S, Baase W, von Hippel P. Mapping the interactions of the single-stranded DNA binding protein of bacteriophage T4 (gp32) with DNA lattices at single nucleotide resolution: gp32 monomer binding. Nucleic Acids Res. 2015;43:9276-90 pubmed publisher
    Combining biophysical measurements on T4 bacteriophage replication complexes with detailed structural information can illuminate the molecular mechanisms of these 'macromolecular machines'...
  35. Krutkina E, Klaiman D, Margalit T, Jerabeck Willemsen M, Kaufmann G. Dual nucleotide specificity determinants of an infection aborting anticodon nuclease. Virology. 2016;487:260-72 pubmed publisher
    The anticodon nuclease (ACNase) PrrC is silenced by a DNA restriction-modification (RM) protein and activated by a phage T4-encoded restriction inhibitor...
  36. Chen J, Yang Y, Zhang L, Liang H, Huber T, Su X, et al. Analysis of the solution conformations of T4 lysozyme by paramagnetic NMR spectroscopy. Phys Chem Chem Phys. 2016;18:5850-9 pubmed publisher
    A large number of crystal structures of bacteriophage T4 lysozyme (T4-L) have shown that it contains two subdomains, which can arrange in a compact conformation (closed state) or, in mutants of T4-L, more extended structures (open state)...
  37. Stortelder A, Buijs J, Bulthuis J, van der Vies S, Gooijer C, van der Zwan G. Time-resolved fluorescence of the bacteriophage T4 capsid protein gp23. J Photochem Photobiol B. 2005;78:53-60 pubmed
    The time-resolved fluorescence properties of the bacteriophage T4 capsid protein gp23 are investigated...
  38. Brok Volchanskaya V, Kadyrov F, Sivogrivov D, Kolosov P, Sokolov A, Shlyapnikov M, et al. Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage. Nucleic Acids Res. 2008;36:2094-105 pubmed publisher
    ..b>Bacteriophage T4 segB gene, which is located in a cluster of tRNA genes, encodes a protein of unknown function, homologous to ..
  39. Kondabagil K, Dai L, Vafabakhsh R, Ha T, Draper B, Rao V. Designing a nine cysteine-less DNA packaging motor from bacteriophage T4 reveals new insights into ATPase structure and function. Virology. 2014;468-470:660-668 pubmed publisher
    The packaging motor of bacteriophage T4 translocates DNA into the capsid at a rate of up to 2000 bp/s. Such a high rate would require coordination of motor movements at millisecond timescale...
  40. Bitton L, Klaiman D, Kaufmann G. Phage T4-induced DNA breaks activate a tRNA repair-defying anticodon nuclease. Mol Microbiol. 2015;97:898-910 pubmed publisher
    ..bacterial anticodon nuclease (ACNase) RloC is not known, but traits that set it apart from the homologous phage T4-excluding ACNase PrrC could provide relevant clues...
  41. Mahalakshmi R, Maurya S, Burdak B, Surti P, Patel M, Jain V. Structural plasticity of T4 transcription co-activator gp33 revealed by a protease-resistant unfolded state. Biochem Biophys Res Commun. 2017;492:61-66 pubmed publisher
    Gene 33 protein (gp33) is a transcriptional coactivator for late genes of the T4 bacteriophage. gp33 possesses a 5-helix bundle core, with unstructured N- and C-terminal regions that account for >50% of the protein sequence...
  42. Chen Z, Sun L, Zhang Z, Fokine A, Padilla Sanchez V, Hanein D, et al. Cryo-EM structure of the bacteriophage T4 isometric head at 3.3-Å resolution and its relevance to the assembly of icosahedral viruses. Proc Natl Acad Sci U S A. 2017;114:E8184-E8193 pubmed publisher
    The 3.3-Å cryo-EM structure of the 860-Å-diameter isometric mutant bacteriophage T4 capsid has been determined...
  43. Brzozowska E, Lesniewski A, Sek S, Wieneke R, Tampe R, Górska S, et al. Interactions of bacteriophage T4 adhesin with selected lipopolysaccharides studied using atomic force microscopy. Sci Rep. 2018;8:10935 pubmed publisher
    The interaction between the T4 bacteriophage gp37 adhesin and the bacterial lipopolysaccharide (LPS) is a well-studied system, however, the affinity and strength of the interaction haven't been analyzed so far...
  44. Migliori A, Smith D, Arya G. Molecular interactions and residues involved in force generation in the T4 viral DNA packaging motor. J Mol Biol. 2014;426:4002-17 pubmed publisher
    ..A model based on recently resolved structures of the bacteriophage T4 motor protein gp17 suggests that this motor generates large forces by undergoing a conformational change from ..
  45. Black L. Old, new, and widely true: The bacteriophage T4 DNA packaging mechanism. Virology. 2015;479-480:650-6 pubmed publisher
    ..Recent fluorescence studies of phage T4 reveal: 1) the small terminase subunit (TerS) synapses pac homologs by a twin ring mechanism to gauge DNA ..
  46. James T, Cardozo T, Abell L, Hsieh M, Jenkins L, Jha S, et al. Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase. Nucleic Acids Res. 2016;44:7974-88 pubmed publisher
    ..In a process called ? appropriation, bacteriophage T4 activates a class of phage promoters using an activator (MotA) and a co-activator (AsiA), which function ..
  47. Minakhin L, Severinov K. Transcription regulation by bacteriophage T4 AsiA. Protein Expr Purif. 2005;41:1-8 pubmed
    b>Bacteriophage T4 AsiA, a strong inhibitor of bacterial RNA polymerase, was the first antisigma protein to be discovered...
  48. Karpel R. DNA Binding Proteins and Drug Delivery Vehicles: Tales of Elephants and Snakes. Curr Protein Pept Sci. 2015;16:718-26 pubmed
    We compare the DNA-interactive properties of bacteriophage T4 gene 32 protein (gp32) with those of crotamine, a component of the venom of the South American rattlesnake...
  49. Lipson S, Karalis G, Karthikeyan L, Ozen F, Gordon R, Ponnala S, et al. Mechanism of Anti-rotavirus Synergistic Activity by Epigallocatechin Gallate and a Proanthocyanidin-Containing Nutraceutical. Food Environ Virol. 2017;9:434-443 pubmed publisher
    ..Coliphage T4II (phage T4) and the rotavirus strain SA-11(RTV) were used as model virus systems...
  50. Hyman P, van Raaij M. Bacteriophage T4 long tail fiber domains. Biophys Rev. 2018;10:463-471 pubmed publisher
    b>Bacteriophage T4 initially recognizes its host cells using its long tail fibers. Long tail fibers consist of a phage-proximal and a phage-distal rod, each around 80 nm long and attached to each other at a slight angle...
  51. Piesiniene L, Truncaite L, Zajanckauskaite A, Nivinskas R. The sequences and activities of RegB endoribonucleases of T4-related bacteriophages. Nucleic Acids Res. 2004;32:5582-95 pubmed publisher
    The RegB endoribonuclease encoded by bacteriophage T4 is a unique sequence-specific nuclease that cleaves in the middle of GGAG or, in a few cases, GGAU tetranucleotides, preferentially those found in the Shine-Dalgarno regions of early ..
  52. Ang D, Georgopoulos C. An ORFan no more: the bacteriophage T4 39.2 gene product, NwgI, modulates GroEL chaperone function. Genetics. 2012;190:989-1000 pubmed publisher
    ..2 in bacteriophages T4, RB43, and RB49 similarly modulate GroEL/GroES. Production of wild-type bacteriophage T4 Gp39...
  53. Moreira S, Noutahi E, Lamoureux G, Burger G. Three-dimensional structure model and predicted ATP interaction rewiring of a deviant RNA ligase 2. BMC Struct Biol. 2015;15:20 pubmed publisher
    ..RNA editing ligase from the parasitic trypanosomes (Kinetoplastea), a promising drug target, and bacteriophage T4 RNA ligase 2, a workhorse in molecular biology...
  54. Wang C, Sauvageau D, Elias A. Immobilization of Active Bacteriophages on Polyhydroxyalkanoate Surfaces. ACS Appl Mater Interfaces. 2016;8:1128-38 pubmed publisher
    ..Modified surfaces were immersed in suspensions of phage T4 for immobilization...
  55. Zhuang Z, Berdis A, Benkovic S. An alternative clamp loading pathway via the T4 clamp loader gp44/62-DNA complex. Biochemistry. 2006;45:7976-89 pubmed publisher
    In bacteriophage T4, a clamp loading pathway that utilizes the T4 clamp loader (gp44/62) and ATP hydrolysis initially to form a complex with the clamp (gp45) has been demonstrated, followed by interaction with DNA and closing of the clamp...
  56. Kumar Sarkar S, Takeda Y, Kanamaru S, Arisaka F. Association and dissociation of the cell puncturing complex of bacteriophage T4 is controlled by both pH and temperature. Biochim Biophys Acta. 2006;1764:1487-92 pubmed
    The tail lysozyme, gp5, of bacteriophage T4 is a trimeric protein and all the subunits are nicked between Ser351 and Ala352 during assembly through processing...
  57. Trzemecka A, Płochocka D, Bebenek A. Different behaviors in vivo of mutations in the beta hairpin loop of the DNA polymerases of the closely related phages T4 and RB69. J Mol Biol. 2009;389:797-807 pubmed publisher
    The T4 and RB69 DNA replicative polymerases are members of the B family and are highly similar...
  58. Shank E, Cecconi C, Dill J, Marqusee S, Bustamante C. The folding cooperativity of a protein is controlled by its chain topology. Nature. 2010;465:637-40 pubmed publisher
    ..Here we use a single-molecule optical tweezers approach to induce the selective unfolding of particular regions of T4 lysozyme and monitor the effect on other regions not directly acted on by force...
  59. White E, Miranker A. A solenoid design for assessing determinants of parallel β-sheet registration. Protein Eng Des Sel. 2015;28:577-83 pubmed publisher
    ..that combines a homotrimeric, triple-stranded β-helix as a guest to a homotrimeric foldon unit from bacteriophage T4 fibritin...
  60. Wei Y, Gao Z, Zhang H, Dong Y. Structural characterizations of phage antitoxin Dmd and its interactions with bacterial toxin RnlA. Biochem Biophys Res Commun. 2016;472:592-7 pubmed publisher
    ..After the infection of bacteriophage T4 with Dmd defection, RnlA is activated by the disappearance of RnlB, resulting in the rapid degradation of T4 ..
  61. Blair L, Tackett A, Raney K. Development and evaluation of a structural model for SF1B helicase Dda. Biochemistry. 2009;48:2321-9 pubmed publisher
    Helicases are proteins that unwind double-stranded nucleic acids. Dda helicase from bacteriophage T4 has served as an excellent model for understanding the molecular mechanism of this class of enzymes...
  62. Prilipov A, Selivanov N, Nikolaeva L, Mesyanzhinov V. Nucleotide and deduced amino acid sequence of bacteriophage T4 gene wac. Nucleic Acids Res. 1988;16:10361 pubmed
  63. Selivanov N, Prilipov A, Mesyanzhinov V. Nucleotide and deduced amino acid sequence of bacteriophage T4 gene 12. Nucleic Acids Res. 1988;16:2334 pubmed
  64. Oliver D, Crowther R. DNA sequence of the tail fibre genes 36 and 37 of bacteriophage T4. J Mol Biol. 1981;153:545-68 pubmed
  65. Horton J, Liebert K, Hattman S, Jeltsch A, Cheng X. Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase. Cell. 2005;121:349-61 pubmed publisher
    ..Three structures are described for bacteriophage T4 DNA-adenine methyltransferase (T4Dam) in ternary complexes with partially and fully specific DNA and a methyl-..
  66. Byrd A, Raney K. Displacement of a DNA binding protein by Dda helicase. Nucleic Acids Res. 2006;34:3020-9 pubmed publisher
    b>Bacteriophage T4 Dda helicase has recently been shown to be active as a monomer for unwinding of short duplex oligonucleotides and for displacing streptavidin from 3'-biotinylated oligonucleotides...
  67. Wang G, Liu J, Kimura M. [Genetic diversity of major capsid genes (g23) of T4-type bacteriophages in natural environments--a review]. Wei Sheng Wu Xue Bao. 2011;51:732-9 pubmed
    ..In this paper, we targeted the major capsid genes (g23) of T4-type bacteriophages and reviewed the recent progress on their genetic diversity...
  68. Manosas M, Perumal S, Bianco P, BIANCO P, Ritort F, Benkovic S, et al. RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue. Nat Commun. 2013;4:2368 pubmed publisher
    ..destabilize a DNA molecule and follow, in real time, unwinding and rewinding by two DNA repair helicases, bacteriophage T4 UvsW and Escherichia coli RecG...
  69. Herdendorf T, Nelson S. Catalytic mechanism of bacteriophage T4 Rad50 ATP hydrolysis. Biochemistry. 2014;53:5647-60 pubmed publisher
    ..Here we examine the kinetic and catalytic mechanism of ATP hydrolysis by T4 Rad50 (gp46) in the presence and absence of Mre11 (gp47) and DNA...
  70. Tao P, Mahalingam M, Rao V. Highly Effective Soluble and Bacteriophage T4 Nanoparticle Plague Vaccines Against Yersinia pestis. Methods Mol Biol. 2016;1403:499-518 pubmed publisher
    ..We have developed two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, to construct new plague vaccines that may overcome these problems...
  71. Prilipov A, Selivanov N, Efimov V, Marusich E, Mesyanzhinov V. Nucleotide sequences of bacteriophage T4 genes 9, 10 and 11. Nucleic Acids Res. 1989;17:3303 pubmed
  72. Arisaka F, Ishimoto L, Kassavetis G, Kumazaki T, Ishii S. Nucleotide sequence of the tail tube structural gene of bacteriophage T4. J Virol. 1988;62:882-6 pubmed
    The nucleotide sequence of gene 19 of bacteriophage T4, the structural gene of the tail tube protein, was determined by both the dideoxy and the Maxam-Gilbert methods. The predicted Mr of tube protein gene product 19 is 18,842...
  73. Amegbey G, Monzavi H, Habibi Nazhad B, Bhattacharyya S, Wishart D. Structural and functional characterization of a thioredoxin-like protein (Mt0807) from Methanobacterium thermoautotrophicum. Biochemistry. 2003;42:8001-10 pubmed
    ..thermoautotrophicum, while Mt0895 plays a minor or supportive role. We also suggest that these two molecules (Mt0807 and Mt0895) may represent a group of ancient proteins that were ancestral to both thioredoxins and glutaredoxins. ..
  74. Yap M, Mio K, Ali S, Minton A, Kanamaru S, Arisaka F. Sequential assembly of the wedge of the baseplate of phage T4 in the presence and absence of gp11 as monitored by analytical ultracentrifugation. Macromol Biosci. 2010;10:808-13 pubmed publisher
    The baseplate wedge of bacteriophage T4 consists of seven gene products, namely, gp11, gp10, gp7, gp8, gp6, gp53, and gp25, which assemble strictly in this order with an exception that gp11 can bind to gp10 at any stage of the assembly...
  75. Naka K, Qi D, Yonesaki T, Otsuka Y. RnlB Antitoxin of the Escherichia coli RnlA-RnlB Toxin-Antitoxin Module Requires RNase HI for Inhibition of RnlA Toxin Activity. Toxins (Basel). 2017;9: pubmed publisher
    ..After bacteriophage T4 infection, RnlA is activated by the disappearance of RnlB, resulting in the rapid degradation of T4 mRNAs and ..
  76. Zheng W, Wang F, Taylor N, Guerrero Ferreira R, Leiman P, Egelman E. Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit. Structure. 2017;25:1436-1441.e2 pubmed publisher
    The bacteriophage T4 contractile tail (containing a tube and sheath) was the first biological assembly reconstructed in three dimensions by electron microscopy at a resolution of ?35 Å in 1968...
  77. Vlot M, Houkes J, Lochs S, Swarts D, Zheng P, Künne T, et al. Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes. Nucleic Acids Res. 2018;46:873-885 pubmed publisher
    ..We show that glucosyl modification of 5-hydroxymethylated cytosines in the DNA of bacteriophage T4 interferes with type I-E and type II-A CRISPR-Cas systems by lowering the affinity of the Cascade and Cas9-..
  78. Piepenburg O, Williams C, Stemple D, Armes N. DNA detection using recombination proteins. PLoS Biol. 2006;4:e204 pubmed publisher
    ..This unique combination of properties is a significant advance in the development of portable and widely accessible nucleic acid-based tests...
  79. Granell M, Namura M, Alvira S, Kanamaru S, van Raaij M. Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34. Viruses. 2017;9: pubmed publisher
    Long tail fibers of bacteriophage T4 are formed by proteins gp34, gp35, gp36, and gp37, with gp34 located at the phage-proximal end and gp37 at the phage-distal, receptor-binding end...
  80. Delagoutte E, von Hippel P. Mechanistic studies of the T4 DNA (gp41) replication helicase: functional interactions of the C-terminal Tails of the helicase subunits with the T4 (gp59) helicase loader protein. J Mol Biol. 2005;347:257-75 pubmed publisher
    We compare the activities of the wild-type (gp41WT) and mutant (gp41delta C20) forms of the bacteriophage T4 replication helicase...
  81. Rouzina I, Pant K, Karpel R, Williams M. Theory of electrostatically regulated binding of T4 gene 32 protein to single- and double-stranded DNA. Biophys J. 2005;89:1941-56 pubmed publisher
    b>Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA binding protein, which is essential for DNA replication, recombination, and repair...
  82. Clare D, Bakkes P, van Heerikhuizen H, van der Vies S, Saibil H. An expanded protein folding cage in the GroEL-gp31 complex. J Mol Biol. 2006;358:905-11 pubmed publisher
    b>Bacteriophage T4 produces a GroES analogue, gp31, which cooperates with the Escherichia coli GroEL to fold its major coat protein gp23...
  83. Lohman G, Chen L, Evans T. Kinetic characterization of single strand break ligation in duplex DNA by T4 DNA ligase. J Biol Chem. 2011;286:44187-96 pubmed publisher
    b>T4 DNA ligase catalyzes phosphodiester bond formation between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA in three steps: 1) enzyme-adenylylate formation by reaction with ATP; 2) adenylyl transfer to a 5'-phosphorylated ..
  84. Inaba H, Ueno T. Artificial bio-nanomachines based on protein needles derived from bacteriophage T4. Biophys Rev. 2018;10:641-658 pubmed publisher
    b>Bacteriophage T4 is a natural bio-nanomachine which achieves efficient infection of host cells via cooperative motion of specific three-dimensional protein architectures...
  85. Xu W, Gauss P, Shen J, Dunn C, Bessman M. The gene e.1 (nudE.1) of T4 bacteriophage designates a new member of the Nudix hydrolase superfamily active on flavin adenine dinucleotide, adenosine 5'-triphospho-5'-adenosine, and ADP-ribose. J Biol Chem. 2002;277:23181-5 pubmed publisher
    The T4 bacteriophage gene e...
  86. Ueno H, Yonesaki T. Phage-induced change in the stability of mRNAs. Virology. 2004;329:134-41 pubmed publisher
    The stability of mRNA in Escherichia coli cells changed after phage T4 infection. Stable E. coli mRNAs such as lpp and ompA were drastically destabilized immediately after infection...
  87. Qu Y, Hyman P, Harrah T, Goldberg E. In vivo bypass of chaperone by extended coiled-coil motif in T4 tail fiber. J Bacteriol. 2004;186:8363-9 pubmed publisher
    The distal-half tail fiber of bacteriophage T4 is made of three gene products: trimeric gp36 and gp37 and monomeric gp35...
  88. Smiley R, Zhuang Z, Benkovic S, Hammes G. Single-molecule investigation of the T4 bacteriophage DNA polymerase holoenzyme: multiple pathways of holoenzyme formation. Biochemistry. 2006;45:7990-7 pubmed publisher
    In T4 bacteriophage, the DNA polymerase holoenzyme is responsible for accurate and processive DNA synthesis. The holoenzyme consists of DNA polymerase gp43 and clamp protein gp45...
  89. Dabrowska K, Zembala M, Boratynski J, Switala Jelen K, Wietrzyk J, Opolski A, et al. Hoc protein regulates the biological effects of T4 phage in mammals. Arch Microbiol. 2007;187:489-98 pubmed publisher
    We previously investigated the biological, non-antibacterial effects of bacteriophage T4 in mammals (binding to cancer cells in vitro and attenuating tumour growth and metastases in vivo); we selected the phage mutant HAP1 that was ..
  90. Liu Q, Dansereau J, Puttamadappa S, Shekhtman A, Derbyshire V, Belfort M. Role of the interdomain linker in distance determination for remote cleavage by homing endonuclease I-TevI. J Mol Biol. 2008;379:1094-106 pubmed publisher
    ..Mutation of this arginine to alanine abolished distance determination and disrupted the beta-strand, indicating that the structure of the DT linker region has a role in cleavage at a fixed distance...
  91. Lagerb ck P, Carlson K. Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis. J Bacteriol. 2008;190:5533-44 pubmed publisher
    b>Phage T4 endonuclease II (EndoII), a GIY-YIG endonuclease lacking a carboxy-terminal DNA-binding domain, was subjected to site-directed mutagenesis to investigate roles of individual amino acids in substrate recognition, binding, and ..
  92. Farb J, Morrical S. Functional complementation of UvsX and UvsY mutations in the mediation of T4 homologous recombination. Nucleic Acids Res. 2009;37:2336-45 pubmed publisher
    b>Bacteriophage T4 homologous recombination events are promoted by presynaptic filaments of UvsX recombinase bound to single-stranded DNA (ssDNA)...