Bacteriophage T5

Summary

Alias: Enterobacteria phage T5, phage T5

Top Publications

  1. Andraos N, Tabor S, Richardson C. The highly processive DNA polymerase of bacteriophage T5. Role of the unique N and C termini. J Biol Chem. 2004;279:50609-18 pubmed publisher
    The DNA polymerase encoded by bacteriophage T5 has been reported previously to be processive and to catalyze extensive strand displacement synthesis...
  2. Huet A, Conway J, Letellier L, Boulanger P. In vitro assembly of the T=13 procapsid of bacteriophage T5 with its scaffolding domain. J Virol. 2010;84:9350-8 pubmed publisher
    ..b>Phage T5 is the first example of a viral capsid consisting of quasi-equivalent hexamers and pentamers whose assembly can ..
  3. Beckman L, Hoffman M, McCorquodale D. Pre-early proteins of bacteriophage T5: structure and function. J Mol Biol. 1971;62:551-64 pubmed
  4. Zweig M, Cummings D. Structural proteins of bacteriophage T5. Virology. 1973;51:443-53 pubmed
  5. Hendrickson H, McCorquodale D. Genetic and physiological studies of bacteriophage T5. 3. Patterns of deoxyribonucleic acid synthesis induced by mutants of T5 and the identification of genes influencing the appearance of phage-induced dihydrofolate reductase and deoxyribonuclease. J Virol. 1972;9:981-9 pubmed
    ..The structural genes for an early T5 deoxyribonuclease and for T5 DNA polymerase, as well as a gene that affects the synthesis of dihydrofolate reductase, have been identified in the genetic map of T5...
  6. McCorquodale D, Chen C, Joseph M, Woychik R. Modification of RNA polymerase from Escherichia coli by pre-early gene products of bacteriophage T5. J Virol. 1981;40:958-62 pubmed
    ..This RNA polymerase complex had altered transcriptional specificity, in that it transcribed pre-early genes less efficiently than it did early genes...
  7. Garforth S, Sayers J. Structure-specific DNA binding by bacteriophage T5 5'-->3' exonuclease. Nucleic Acids Res. 1997;25:3801-7 pubmed
    b>Phage T5 exonuclease is a 5'-->3'exodeoxyribonuclease that also exhibits endonucleolytic activity on flap structures (branched duplex DNA containing a free single-stranded 5'-end)...
  8. Garforth S, Ceska T, Suck D, Sayers J. Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage. Proc Natl Acad Sci U S A. 1999;96:38-43 pubmed
    ..investigate the roles of three lysine residues (K83, K196, and K215) situated near two metal-binding sites in bacteriophage T5 5'-3' exonuclease...
  9. Mikoulinskaia G, Zimin A, Feofanov S, Miroshnikov A. Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13). Protein Expr Purif. 2004;33:166-75 pubmed
    The nucleotide sequence corresponding to 13-19.5% of the bacteriophage T5 genome in early region C was determined (GenBank AY 140897)...

More Information

Publications100

  1. Steigemann B, Schulz A, Werten S. Bacteriophage T5 encodes a homolog of the eukaryotic transcription coactivator PC4 implicated in recombination-dependent DNA replication. J Mol Biol. 2013;425:4125-33 pubmed publisher
  2. Kovalenko A, Chernyshov S, Kutyshenko V, Molochkov N, Prokhorov D, Odinokova I, et al. Investigation of the calcium-induced activation of the bacteriophage T5 peptidoglycan hydrolase promoting host cell lysis. Metallomics. 2019;11:799-809 pubmed publisher
    Peptidoglycan hydrolase of bacteriophage T5 (EndoT5) is a Ca2+-dependent l-alanyl-d-glutamate peptidase, although the mode of Ca2+ binding and its physiological significance remain obscure...
  3. Kutyshenko V, Mikoulinskaia G, Chernyshov S, Yegorov A, Prokhorov D, Uversky V. Effect of C-terminal His-tag and purification routine on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of the bacteriophage T5. Int J Biol Macromol. 2019;124:810-818 pubmed publisher
    ..chromatography (IMAC) on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of bacteriophage T5 (EndoT5), whose zinc binding site and catalytic aspartate are located near the C-terminus...
  4. Dominguez Medina S, Fostner S, Defoort M, Sansa M, Stark A, Halim M, et al. Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators. Science. 2018;362:918-922 pubmed publisher
    ..with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument ..
  5. Golomidova A, Kulikov E, Prokhorov N, Guerrero Ferreira R, Ksenzenko V, Tarasyan K, et al. Complete genome sequences of T5-related Escherichia coli bacteriophages DT57C and DT571/2 isolated from horse feces. Arch Virol. 2015;160:3133-7 pubmed publisher
    ..protein genes (mostly of those with unknown functions) as well as substantial differences in tail fiber locus organization support the conclusion that DT57C and DT571/2 form a species-level group clearly distinct from bacteriophage T5.
  6. Preux O, Durand D, Huet A, Conway J, Bertin A, Boulogne C, et al. A two-state cooperative expansion converts the procapsid shell of bacteriophage T5 into a highly stable capsid isomorphous to the final virion head. J Mol Biol. 2013;425:1999-2014 pubmed publisher
    ..Here, we report on the expansion process of the large capsid (T=13) of bacteriophage T5. We purified the intermediate prohead II form, which is competent for packaging the 121-kbp dsDNA genome, and ..
  7. Mortusewicz O, Evers B, Helleday T. PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites. Oncogene. 2016;35:761-70 pubmed publisher
    ..transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein...
  8. Fujimura R, Tavtigian S, Choy T, Roop B. Physical locus of the DNA polymerase gene and genetic maps of bacteriophage T5 mutants. J Virol. 1985;53:495-500 pubmed
    Segments of DNA that contained the DNA polymerase gene of bacteriophage T5 were isolated...
  9. Gruber S, Hagen J, Schwab H, Koefinger P. Reprint of "versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16". J Biotechnol. 2014;192 Pt B:410-8 pubmed publisher
    ..In particular, the use of promoters derived from the bacteriophage T5 was described for the first time in this work, characterizing the j5 promoter as the strongest promoter yet ..
  10. Snyder C. Bacteriophage T5 gene A2 protein alters the outer membrane of Escherichia coli. J Bacteriol. 1984;160:1191-5 pubmed
    Evidence for changes in Escherichia coli envelope structure caused by the bacteriophage T5 gene A2 protein was obtained by the use of mutant bacteriophages, envelope fractionation procedures, electrophoretic analysis, and in vitro binding ..
  11. Fujimura R, Roop B. Temperature-sensitive DNA polymerase induced by a bacteriophage T5 mutant: relationship between polymerase and exonuclease activities. Biochemistry. 1976;15:4403-9 pubmed
    ..Purified ts53 polymerase was also shown to discriminate against incorportion of BrdUMP, especially at higher temperature. This is an agreement with observations made in vivo with ts53-infected bacteria. ..
  12. Warner H, Thompson R, Mozer T, Duncan B. The properties of a bacteriophage T5 mutant unable to induce deoxyuridine 5'-triphosphate nucleotidohydrolase. Synthesis of uracil-containing T5 deoxyribonucleic acid. J Biol Chem. 1979;254:7534-9 pubmed
    b>Bacteriophage T5 induces a deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) activity during infection of Escherichia coli. A T5 mutant (T5 dut) unable to induce this dUTPase activity has been isolated...
  13. Huet A, Duda R, Hendrix R, Boulanger P, Conway J. Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. J Mol Biol. 2016;428:165-181 pubmed publisher
    ..These results reveal complexity in T5 beyond that of the HK97 system. ..
  14. AlMalki F, Flemming C, Zhang J, Feng M, Sedelnikova S, Ceska T, et al. Direct observation of DNA threading in flap endonuclease complexes. Nat Struct Mol Biol. 2016;23:640-6 pubmed publisher
    ..Here, we report crystal structures of bacteriophage T5 flap endonuclease in complexes with intact DNA substrates and products, at resolutions of 1.9-2.2 Å...
  15. Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, et al. Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components. J Virol. 2014;88:1162-74 pubmed publisher
    b>Bacteriophage T5 represents a large family of lytic Siphoviridae infecting Gram-negative bacteria...
  16. Brunel F, Davison J, Ha Thi V, Reeve J. Cloning of bacteriophage T5 DNA fragments. III. Expression in Escherichia coli mini-cells. Gene. 1981;16:107-18 pubmed
    ..In some cases, it has been possible to demonstrate the presence of T5 promoters on the cloned DNA fragments. The design of experiments to avoid certain artifacts inherent in the use of the mini-cell system is discussed. ..
  17. Krauel V, Heller K. Location of genes D16, D17, and N4 encoding tail proteins on the physical map of bacteriophage T5. Gene. 1987;54:261-5 pubmed
    b>Bacteriophage T5 DNA fragments were cloned into plasmid pBR322. Recombinant plasmids complementing T5 amber mutants were isolated, and used as hybridization probes with T5 DNA in Southern blots...
  18. Golomidova A, Kulikov E, Prokhorov N, Guerrero Ferreira R, Knirel Y, Kostryukova E, et al. Branched Lateral Tail Fiber Organization in T5-Like Bacteriophages DT57C and DT571/2 is Revealed by Genetic and Functional Analysis. Viruses. 2016;8: pubmed publisher
    ..L-shaped tail fibers), however, are composed of two proteins, LtfA and LtfB, instead of the single Ltf of bacteriophage T5. In silico and mutant analysis suggests a possible branched structure of DT57C and DT571/2 LTFs, where the ..
  19. Noirclerc Savoye M, Flayhan A, Pereira C, Gallet B, Gans P, Ebel C, et al. Tail proteins of phage T5: investigation of the effect of the His6-tag position, from expression to crystallisation. Protein Expr Purif. 2015;109:70-8 pubmed publisher
    Upon binding to its bacterial host receptor, the tail tip of phage T5 perforates, by an unknown mechanism, the heavily armoured cell wall of the host...
  20. Pickering T, Garforth S, Sayers J, Grasby J. Variation in the steady state kinetic parameters of wild type and mutant T5 5'-3'-exonuclease with pH. Protonation of Lys-83 is critical for DNA binding. J Biol Chem. 1999;274:17711-7 pubmed
    ..We therefore conclude that protonation of Lys-83 in the wild type protein facilitates DNA binding. The origin of the pH dependence of the kcat parameter of the wild type enzyme is discussed. ..
  21. Eriksson S, Berglund O. Bacteriophage-induced ribonucleotide reductase systems. T5- and T6-specific ribonucleotide reductase and thioredoxin. Eur J Biochem. 1974;46:271-8 pubmed
  22. Duckworth D, Dunn G, McCorquodale D. Identification of the gene controlling the synthesis of the major bacteriophage T5 membrane protein. J Virol. 1976;18:542-9 pubmed
    After infection of Escherichia coli B by bacteriophage T5, a major new protein species, as indicated by polyacrylamide gel electrophoresis, appears in the cells' membranes...
  23. Decker K, Krauel V, Meesmann A, Heller K. Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the FhuA receptor protein by a lipoprotein expressed early during infection. Mol Microbiol. 1994;12:321-32 pubmed
    ..the region between the oad gene, encoding the host specificity protein, and the right-terminal repetition of bacteriophage T5 DNA was determined...
  24. Bessman M, HERRIOTT S, Orr M. THE ENZYMOLOGY OF VIRUS-INFECTED BACTERIA. VI. PURIFICATION AND PROPERTIES OF THE DEOXYNUCLEOTIDE KINASE INDUCED BY BACTERIOPHAGE T5. J Biol Chem. 1965;240:439-45 pubmed
  25. Shliapnikov M, Kriukov V, Baev A. [Primary structure of tRNAAsn of bacteriophage T5]. Bioorg Khim. 1987;13:533-8 pubmed
    Unformly 32P-labelled phage-specific tRNAAsn was isolated from bacteriophage T5-infected E...
  26. Rice A, Ficht T, Holladay L, Moyer R. The purification and properties of a double-stranded DNA-binding protein encoded by the gene D5 of bacteriophage T5. J Biol Chem. 1979;254:8042-51 pubmed
    We have purified a DNA-binding protein from bacteriophage T5-infected cells. The protein is the product of the T5 gene D5 and is produced in quantities ultimately exceeding 2% of the total cell protein...
  27. Leavitt M, Ito J. T5 DNA polymerase: structural--functional relationships to other DNA polymerases. Proc Natl Acad Sci U S A. 1989;86:4465-9 pubmed
    ..These shared regions have been implicated in the 3'----5' exonuclease function of polymerase I, which suggests that the proofreading domains of all these enzymes may be evolutionarily related. ..
  28. Heller K, Bryniok D. O antigen-dependent mutant of bacteriophage T5. J Virol. 1984;49:20-5 pubmed
  29. Fujimura R, Roop B. Characterization of DNA polymerase induced by bacteriophage T5 with DNA containing single strand breaks. J Biol Chem. 1976;251:2168-74 pubmed
    DNA polymerase induced by bacteriophage T5 was purified and characterized using mainly circular duplex DNA of bacteriophage PM2 with single strand breaks formed by DNase I action...
  30. Ksenzenko V, Wilkens K, Rueger W, Shlyapnikov M. Nucleotide sequence of the phage T5 DNA segment containing six tRNA genes. Nucleic Acids Res. 1992;20:6104 pubmed
  31. Mozer T, Warner H. Properties of deoxynucleoside 5'-monophosphatase induced by bacteriophage T5 after infection of Escherichia coli. J Virol. 1977;24:635-41 pubmed
    b>Bacteriophage T5 induced a deoxynucleoside 5'-monophosphatase during its infection of Escherichia coli. The enzyme was purified about 100-fold...
  32. Das S, Fujimura R. Mechanism of 3' to 5' exonuclease associated with phage T5-induced DNA polymerase: processiveness and template specificity. Nucleic Acids Res. 1980;8:657-71 pubmed
    ..Control experiments with complementary base residues at the 3'-end indicate that extensive removal of terminal residue takes place in the presence of dNTP's only when such residues are "improper" in the Watson-Crick sense. ..
  33. Kaliman A, Kryukov V, Bayev A. The nucleotide sequence of bacteriophage T5 DNA at the region between early and late genes. Nucleic Acids Res. 1988;16:6230 pubmed
  34. Shlyapnikov M, Ksenzenko V, Kryukov V, Bayev A. Nucleotide sequence of the bacteriophage T5 DNA fragment which contains the gene for tRNAAsp. Eur J Biochem. 1986;156:285-9 pubmed
    The nucleotide sequence of bacteriophage T5 tRNAAsp has been determined by conventional methods using thin-layer chromatography on cellulose for oligonucleotide fractionation...
  35. Berget S, Mozer T, Warner H. Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity. J Virol. 1976;18:71-9 pubmed
    The control of activity of the bacteriophage T5-induced 5'-nucleotidase is dependent upon the amount of T5 parental DNA injected into the cell and expressed...
  36. Braun V, Killmann H, Herrmann C. Inactivation of FhuA at the cell surface of Escherichia coli K-12 by a phage T5 lipoprotein at the periplasmic face of the outer membrane. J Bacteriol. 1994;176:4710-7 pubmed
    Inactivation of phage T5 by lysed cells after phage multiplication is prevented by a phage-encoded lipoprotein (Llp) that inactivates the FhuA outer membrane receptor protein (K. Decker, V. Krauel, A. Meesmann, and K. Heller, Mol...
  37. Lanni Y. Functions of two genes in the first-step-transfer DNA of bacteriophage T5. J Mol Biol. 1969;44:173-83 pubmed
  38. Frenkel G, Richardson C. The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. II. Role of the enzyme in replication of the pahge deoxyribonucleic acid. J Biol Chem. 1971;246:4848-52 pubmed
  39. Kaliman A, Kulshin V, Shlyapnikov M, Ksenzenko V, Kryukov V. The nucleotide sequence of the bacteriophage T5 ltf gene. FEBS Lett. 1995;366:46-8 pubmed
    The nucleotide sequence of the bacteriophage T5 Bg/II-BamHI fragment (4,835 bp in length) known to carry a gene encoding the LTF protein which forms the phage L-shaped tail fibers was determined...
  40. Pedruzzi I, Rosenbusch J, Locher K. Inactivation in vitro of the Escherichia coli outer membrane protein FhuA by a phage T5-encoded lipoprotein. FEMS Microbiol Lett. 1998;168:119-25 pubmed
    b>Bacteriophage T5-encoded lipoprotein, synthesized by infected Escherichia coli cells, prevents superinfection of the host cell by this virus...
  41. Kaliman A, Kryukov V, Bayev A. The nucleotide sequence of the region of bacteriophage T5 early genes D10-D15. Nucleic Acids Res. 1988;16:10353-4 pubmed
  42. Krauel V, Heller K. Cloning, sequencing, and recombinational analysis with bacteriophage BF23 of the bacteriophage T5 oad gene encoding the receptor-binding protein. J Bacteriol. 1991;173:1287-97 pubmed
    Binding of bacteriophage T5 to its receptor, the Escherichia coli FhuA protein, is mediated by tail protein pb5...
  43. Herman R, Moyer R. In vivo repair of bacteriophage t5 DNA: an assay for viral growth control. Virology. 1975;66:393-407 pubmed
  44. Frenkel G, Richardson C. The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. I. Characterization of the enzyme as a 5'-exonuclease. J Biol Chem. 1971;246:4839-47 pubmed
  45. Orr C, HERRIOTT S, Bessman M. The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic acid polymerase induced by bacteriophage T5. J Biol Chem. 1965;240:4652-8 pubmed
  46. Fujimura R, Allison D. Electron micrographs of nicked DNA replicated by T5 DNA polymerase. J Biol Chem. 1976;251:2174-5 pubmed
  47. Mikoulinskaia G, Gubanov S, Zimin A, Kolesnikov I, Feofanov S, Miroshnikov A. Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5. Protein Expr Purif. 2003;27:195-201 pubmed
    Deoxynucleoside monophosphate kinase (dNMP kinase) of bacteriophage T5 (EC 2.7.4.13) was purified to apparent homogeneity from phage-infected Escherichia coli cells...
  48. Joannes M, Saucier J, Jacquemin Sablon A. DNA filter retention assay for exonuclease activities. Application to the analysis of processivity of phage T5 induced 5'-exonuclease. Biochemistry. 1985;24:8043-9 pubmed
    The 5'-exonuclease of phage T5 has been purified nearly to homogeneity by using a simple and fast procedure...
  49. Mozer T, Thompson R, Berget S, Warner H. Isolation and characterization of a bacteriophage T5 mutant deficient in deoxynucleoside 5'-monophosphatase activity. J Virol. 1977;24:642-50 pubmed
    A bacteriophage T5 mutant has been isolated that is completely deficient in the induction of deoxynucleoside 5'-monophosphatase activity during infection of Escherichia coli F...
  50. Garcia Doval C, Castón J, Luque D, Granell M, Otero J, Llamas Saiz A, et al. Structure of the Receptor-Binding Carboxy-Terminal Domain of the Bacteriophage T5 L-Shaped Tail Fibre with and without Its Intra-Molecular Chaperone. Viruses. 2015;7:6424-40 pubmed publisher
    b>Bacteriophage T5, a Siphovirus belonging to the order Caudovirales, has a flexible, three-fold symmetric tail, to which three L-shaped fibres are attached...
  51. McCorquodale D, Gossling J, Benzinger R, Chesney R, Lawhorne L, Moyer R. Gene D5 product of bacteriophage T5: DNA-binding protein affecting DNA replication and late gene expression. J Virol. 1979;29:322-7 pubmed
    Gene D5 is not only necessary for replication of bacteriophage T5 DNA and for shutoff of expression of some early genes, but has been found to be necessary also for the expression of late T5 genes...
  52. Pan kova N, Karamyshev A, Shliapnikov M, Presich A, Ksenzenko V. [Amber-suppressive tRNA from bacteriophage T5: construction of genes and determination of the effectiveness of suppression in vivo]. Mol Biol (Mosk). 1998;32:1004-12 pubmed
  53. Fujimura R, Roop B. Interaction of a DNA-binding protein, the product of gene D5 of bacteriophage T5, with double-stranded DNA: effects on T5 DNA polymerase functions in vitro. J Virol. 1983;46:778-87 pubmed
    The gene D5 product (gpD5) of bacteriophage T5 is a DNA-binding protein that binds preferentially to double-stranded DNA and is essential for T5 DNA replication, yet it inhibits DNA synthesis in vitro...
  54. Das S, Fujimura R. Exonuclease associated with bacteriophage T5-Induced DNA polymerase. J Virol. 1976;20:70-7 pubmed
    ..However, the exonuclease activity was operative in both cases under synthetic conditions, as evidenced by template-dependent conversion of [3H]dTTP to [3H]dTMP. ..
  55. Ksenzenko V, Shlyapnikov M, Azbarov V, Garcia O, Kryukov V, Bayev A. Nucleotide sequence of the bacteriophage T5 DNA fragment containing a distal part of tRNA gene region. Nucleic Acids Res. 1987;15:5480-1 pubmed
  56. Fujimura R, Roop B. Interaction of a DNA-binding protein, the gene product of D5 of bacteriophage T5, with double-stranded DNA. Analysis by metrizamide gradient centrifugation. J Biol Chem. 1982;257:14811-6 pubmed
    Interactions of DNA and the gene product D5 (gpD5) of bacteriophage T5, a DNA-binding protein that binds preferentially and cooperatively to double-stranded DNA, were analyzed by metrizamide gradient centrifugation...
  57. BARNER H, Cohen S. Virus-induced acquisition of metabolic function. IV. Thymidylate synthetase in thymine-requiring Escherichia coli infected by T2 and T5 bacteriophages. J Biol Chem. 1959;234:2987-91 pubmed
  58. Das S, Fujimura R. Mechanism of T5-induced DNA polymerase. II. Characterization of the dead-end complex. J Biol Chem. 1977;252:8708-12 pubmed
    We have shown that bacteriophage T5-induced DNA polymerase replicates short primer-templates (400 to 600 nucleotides long) at a rapid rate initially, followed by a slower rate sustained for much longer periods (Das, S. K., and Fujimura, R...
  59. Swart W, Warner H. Isolation and partial characterization of a bacteriophage T5 mutant unable to induce thymidylate synthetase and its use in studying the effect of uracil incorporation into DNA on early gene expression. J Virol. 1985;54:86-91 pubmed
    A mutant of phage T5 which is unable to induce thymidylate synthetase was isolated...
  60. Snyder C, Benzinger R. Second-step transfer of bacteriophage T5 DNA: purification and characterization of the T5 gene A2 protein. J Virol. 1981;40:248-57 pubmed
    Second-step transfer of bacteriophage T5 DNA requires the function of the T5 pre-early proteins A1 and A2. We have isolated and characterized the gene A2 protein as part of an effort to determine the mechanism of second-step transfer...
  61. Warner H, Drong R, Berget S. Early events after infection of Escherichia coli by bacteriophage T5. Induction of a 5'-nucleotidase activity and excretion of free bases. J Virol. 1975;15:273-80 pubmed
    Thymine-containing compounds, produced degradation of Escherichia coli DNA after infection of the cells with bacteriophage T5, did not accumulate in the cell but were excreted into the medium as the DNA was degraded...
  62. Kryukov V, Ksenzenko V, Kaliman A, Bayev A. Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer. FEBS Lett. 1983;158:123-7 pubmed
    b>Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322...
  63. McCorquodale D, Shaw A, Shaw P, Chinnadurai G. Pre-early polypeptides of bacteriophages T5 and BF23. J Virol. 1977;22:480-8 pubmed
    Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23...
  64. Heller K, Schwarz H. Irreversible binding to the receptor of bacteriophages T5 and BF23 does not occur with the tip of the tail. J Bacteriol. 1985;162:621-5 pubmed
    Treatment of purified tails of bacteriophage T5 with 0.05% sodium dodecyl sulfate specifically removed pb2, a protein of 108,000 molecular weight (108K), from the tail...
  65. Das S, Fujimura R. Mechanism of T5-induced DNA polymerase. I. Replication of short primer templates. J Biol Chem. 1977;252:8700-7 pubmed
    b>Bacteriophage T5-induced DNA polymerase shows an initial phase of rapid synthesis, followed by a slower steady rate for much longer periods, with short DNA primer-templates (400 to 600 nucleotides long), in vitro...
  66. Kutyshenko V, Mikoulinskaia G, Molochkov N, Prokhorov D, Taran S, Uversky V. Structure and dynamics of the retro-form of the bacteriophage T5 endolysin. Biochim Biophys Acta. 2016;1864:1281-91 pubmed publisher
    ..NMR spectroscopy we conducted a comparative analysis of the structural and dynamic properties of the bacteriophage T5 endolysin (EndoT5) and its retro-form; i.e...
  67. Shavrina M, Zimin A, Molochkov N, Chernyshov S, Machulin A, Mikoulinskaia G. In vitro study of the antibacterial effect of the bacteriophage T5 thermostable endolysin on Escherichia coli cells. J Appl Microbiol. 2016;121:1282-1290 pubmed publisher
    ..study aimed to evaluate lysis of Escherichia coli stationary cell cultures induced by the combined action of bacteriophage T5 endolysin (l-alanyl-d-glutamate peptidase) and low doses of various cationic agents permeabilizing the outer ..
  68. Das S, Fujimura R. Processiveness of DNA polymerases. A comparative study using a simple procedure. J Biol Chem. 1979;254:1227-32 pubmed
    ..With "nicked DNA-like" poly(dA):oligo(dT), the processiveness of E. coli DNA polymerase I is increased 2- to 2.5-fold. The significance of this increase in determining the "patch size" during DNA repair is discussed. ..
  69. Kaliman A. Identification of the bacteriophage T5 dUTPase by protein sequence comparisons. DNA Seq. 1996;6:347-50 pubmed
    ..by protein sequence comparisons that a 148 amino acid open reading frame (ORF 148) located at 67% of the bacteriophage T5 genome encodes a protein with strong similarity to known dUTPases...
  70. Das S, Fujimura R. Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase. J Biol Chem. 1980;255:7149-54 pubmed
    ..A simple probabilistic model of turnover is discussed...
  71. Chatterjee D, Fujimura R, Campbell J, Gerard G. Cloning and overexpression of the gene encoding bacteriophage T5 DNA polymerase. Gene. 1991;97:13-9 pubmed
    T5 DNA polymerase (T5Pol), an essential enzyme for bacteriophage T5 DNA replication, is unusual because of its high processivity and strand-displacing ability...
  72. Blinov V, Koonin E, Gorbalenya A, Kaliman A, Kryukov V. Two early genes of bacteriophage T5 encode proteins containing an NTP-binding sequence motif and probably involved in DNA replication, recombination and repair. FEBS Lett. 1989;252:47-52 pubmed
    ..It is hypothesized that both D10 and D13 gene products of T5 might be NTPases, possibly DNA-dependent, mediating NTP-consuming steps during phage DNA replication, recombination and/or repair...
  73. Ceska T, Sayers J, Stier G, Suck D. A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease. Nature. 1996;382:90-3 pubmed publisher
    ..Here we report the 2.5-A crystal structure of the phage T5 5'-exonuclease, which reveals a helical arch for binding DNA...
  74. Kaliman A, Krutilina A, Kryukov V, Bayev A. Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Lett. 1986;195:61-4 pubmed
    ..The sequence contains an open reading frame for 291 amino acid residues. The molecular mass of the 5'-exonuclease calculated from the predicted amino acid sequence is 33 400 Da...
  75. Robichon C, Bonhivers M, Pugsley A. An intramolecular disulphide bond reduces the efficacy of a lipoprotein plasma membrane sorting signal. Mol Microbiol. 2003;49:1145-54 pubmed
    ..lipoprotein sorting in Escherichia coli, we devised a novel screen in which sensitivity or resistance to bacteriophage T5 and colicin M reflects the membrane localization of the bacteriophage T5-encoded lipoprotein Llp, which ..
  76. Artymiuk P, Ceska T, Suck D, Sayers J. Prokaryotic 5'-3' exonucleases share a common core structure with gamma-delta resolvase. Nucleic Acids Res. 1997;25:4224-9 pubmed
    ..Different large-scale helical structures are used by both families to form DNA binding sites...
  77. Mondigler M, Holz T, Heller K. Identification of the receptor-binding regions of pb5 proteins of bacteriophages T5 and BF23. Virology. 1996;219:19-28 pubmed
    The receptor-binding protein pb5(T5) of bacteriophage T5, when expressed from the oad gene cloned in pVK88 under the control of the phage T7 promoter/polymerase system, has been shown to bind to its FhuA receptor on the surface of E...
  78. de Waard A, Paul A, Lehman I. The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc Natl Acad Sci U S A. 1965;54:1241-8 pubmed
  79. Mondigler M, Vögele R, Heller K. Overproduced and purified receptor binding protein pb5 of bacteriophage T5 binds to the T5 receptor protein FhuA. FEMS Microbiol Lett. 1995;130:293-300 pubmed
    A promotor-less oad gene of bacteriophage T5, encoding the receptor binding protein pb5, was cloned into pT7-3 under the control of phage T7 promoter phi 10. Induction with IPTG resulted in enhanced production of pb5...
  80. McCorquodale D, Lanni Y. Patterns of protein synthesis in Escherichia coli infected by amber mutants in the first-step-transfer DNA of T5. J Mol Biol. 1970;48:133-43 pubmed
  81. Garforth S, Patel D, Feng M, Sayers J. Unusually wide co-factor tolerance in a metalloenzyme; divalent metal ions modulate endo-exonuclease activity in T5 exonuclease. Nucleic Acids Res. 2001;29:2772-9 pubmed
    ..We suggest that such a substrate may gain access to the active site of the enzyme by a process which does not involve threading...
  82. Plan on L, Janmot C, le Maire M, Desmadril M, Bonhivers M, Letellier L, et al. Characterization of a high-affinity complex between the bacterial outer membrane protein FhuA and the phage T5 protein pb5. J Mol Biol. 2002;318:557-69 pubmed publisher
    Binding of bacteriophage T5 to Escherichia coli cells is mediated by specific interactions between the receptor-binding protein pb5 (67.8 kDa) and the outer membrane iron-transporter FhuA...
  83. Blaisdell P, Warner H. Cell-free transcription and translation of isolated restriction fragments localize bacteriophage T5 pre-early genes. J Virol. 1986;57:759-64 pubmed
    ..and translation of isolated restriction fragments containing all or part of the terminal redundancies of bacteriophage T5 localized virtually every pre-early gene to a small 6.3-kilobase BglI fragment...
  84. Sayers J, Eckstein F. A single-strand specific endonuclease activity copurifies with overexpressed T5 D15 exonuclease. Nucleic Acids Res. 1991;19:4127-32 pubmed
    ..We also report on sequence similarities between the T5 exonuclease and several prokaryotic DNA polymerases...
  85. Pickering T, Garforth S, Thorpe S, Sayers J, Grasby J. A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins. Nucleic Acids Res. 1999;27:730-5 pubmed
    b>Bacteriophage T5 5'-->3' exonuclease is a member of a family of sequence related 5'-nucleases which play an essential role in DNA replication...
  86. Heller K. Identification of the phage gene for host receptor specificity by analyzing hybrid phages of T5 and BF23. Virology. 1984;139:11-21 pubmed
    ..Evidence is presented that this gene is identical to the oad gene previously described (K.J. Heller and D. Bryniok (1984), J. Virol. 49, 20-25). The location of other genes encoding tail proteins is discussed...
  87. Vijayakumar R, Tripathi T. Soluble expression and purification of a full-length asparaginyl tRNA synthetase from Fasciola gigantica. Protein Expr Purif. 2018;143:9-13 pubmed publisher
    ..It was then inserted in the expression vector pQE30 under the transcriptional control of the bacteriophage T5 promoter and lac operator...
  88. Ksenzenko V, Kamynina T, Kazantsev S, Shlyapnikov M, Kryukov V, Bayev A. Cloning of genes for bacteriophage T5 stable RNAs. Biochim Biophys Acta. 1982;697:235-42 pubmed
    ..The arrangement of these genes on the physical map of T5 phage was as follows: -tRNAGln-tRNAHis-RNA III-RNA I-...-tRNAAsp...
  89. Sayers J, Eckstein F. Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease. J Biol Chem. 1990;265:18311-7 pubmed
    The D15 gene of the bacteriophage T5, thought to encode an exonuclease, was cloned into an M13 phage on a 1344-base pair fragment. The deduced amino acid sequence of 291 residues (Kaliman, A. V., Krutilina, A. I., Kryukov, V. M...
  90. Oliveira H, Pinto G, Hendrix H, Noben J, Gawor J, Kropinski A, et al. A Lytic Providencia rettgeri Virus of Potential Therapeutic Value Is a Deep-Branching Member of the T5virus Genus. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..organization, terminal repeats (10,461 bp), and nicks in the 3'-to-5' strand are similar to those of phage T5. However, sequence similarities of PR1 to phages of the T5virus genus at the DNA and protein levels are ..
  91. Arnaud C, Effantin G, Vivès C, Engilberge S, Bacia M, Boulanger P, et al. Bacteriophage T5 tail tube structure suggests a trigger mechanism for Siphoviridae DNA ejection. Nat Commun. 2017;8:1953 pubmed publisher
    ..Comparison of these two structures reveals that host-binding information is not propagated to the capsid through conformational changes in the tail tube, suggesting a role of the TMP in this information transduction process. ..