Lentivirus

Summary

Alias: Lentivirinae

Top Publications

  1. Malur A, Huizar I, Wells G, Barna B, Malur A, Thomassen M. Lentivirus-ABCG1 instillation reduces lipid accumulation and improves lung compliance in GM-CSF knock-out mice. Biochem Biophys Res Commun. 2011;415:288-93 pubmed publisher
    ..We reported previously that intratracheal instillation of a lentivirus (lenti)-PPAR? plasmid into GM-CSF KO animals elevated ABCG1 and reduced alveolar macrophage lipid accumulation...
  2. He C, Yu T, Shi Y, Ma C, Yang W, Fang L, et al. MicroRNA 301A Promotes Intestinal Inflammation and Colitis-Associated Cancer Development by Inhibiting BTG1. Gastroenterology. 2017;152:1434-1448.e15 pubmed publisher
    ..Human colon cancer cell lines (HCT-116 and SW480) were transfected with a lentivirus that expresses MIR301A; expression of cytokines and tight junction proteins were measured by quantitative ..
  3. Zheng F, Han J, Lu H, Cui C, Yang J, Cui Q, et al. Cystathionine beta synthase-hydrogen sulfide system in paraventricular nucleus reduced high fatty diet induced obesity and insulin resistance by brain-adipose axis. Biochim Biophys Acta Mol Basis Dis. 2018;: pubmed publisher
    ..In HFD induced obese rats, CBS protein of hypothalamus decreased. By contrast, overexpression CBS in PVN via lentivirus, lowered food uptake, body weight and fat mass, and reduced blood glucose, lipid disorders and insulin ..
  4. Zhao L, Jiang X, Shi J, Gao S, Zhu Y, Gu T, et al. Exosomes derived from bone marrow mesenchymal stem cells overexpressing microRNA-25 protect spinal cords against transient ischemia. J Thorac Cardiovasc Surg. 2019;157:508-517 pubmed publisher
    ..Cultured mesenchymal stem cells were transfected with lentivirus vectors containing pre-microRNA-25 or control vectors. Exosomes were isolated and harvested by centrifugation...
  5. Du C, Ma J, Liu Q, Li Y, He X, Lin Y, et al. Mice transgenic for equine cyclin T1 and ELR1 are susceptible to equine infectious anemia virus infection. Retrovirology. 2015;12:36 pubmed publisher
    As a member of the tumor necrosis factor receptor (TNFR) protein superfamily, equine lentivirus receptor 1 (ELR1) has been shown to be expressed in various equine cells that are permissive for equine infectious anemia virus (EIAV) ..
  6. Han X, Zhang F, Tan X, Liu L, Ma W, Ou Yang H, et al. EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model. J Cell Biochem. 2018;119:1008-1016 pubmed publisher
    ..EPO gene was transfected into MSCs via lentivirus vector...
  7. Qin G, Zhou Y, Guo F, Ren L, Wu L, Zhang Y, et al. Overexpression of the FoxO1 Ameliorates Mesangial Cell Dysfunction in Male Diabetic Rats. Mol Endocrinol. 2015;29:1080-91 pubmed
    ..After overexpression of constitutively active (CA) FoxO1 in vivo and in vitro by using lentivirus vector, in vivo and in vitro, FoxO1 expression and activity was increased, in accordance with up-regulation of ..
  8. Chen N, Cen J, Wang J, Qin G, Long L, Wang L, et al. Targeted Inhibition of Leucine-Rich Repeat and Immunoglobulin Domain-Containing Protein 1 in Transplanted Neural Stem Cells Promotes Neuronal Differentiation and Functional Recovery in Rats Subjected to Spinal Cord Injury. Crit Care Med. 2016;44:e146-57 pubmed publisher
    ..neural stem cells were divided into four groups for the following treatments: control, control RNA interference lentivirus, LINGO-1 RNA interference lentivirus and LINGO-1 complementary DNA lentivirusand the Key Projects of the Natural ..
  9. Yang S, Gao L, Lu F, Wang B, Gao F, Zhu G, et al. Transcription factor myocyte enhancer factor 2D regulates interleukin-10 production in microglia to protect neuronal cells from inflammation-induced death. J Neuroinflammation. 2015;12:33 pubmed publisher
    ..Recombinant lentivirus expressing shRNA specific to MEF2D was used to silence MEF2D expression in BV2 cells...

More Information

Publications310 found, 100 shown here

  1. Luo L, Fang K, Dan X, Gu M. Crocin ameliorates hepatic steatosis through activation of AMPK signaling in db/db mice. Lipids Health Dis. 2019;18:11 pubmed publisher
    ..db/db mice were administrated with crocin and injected with LV-shAMPK or its negative control lentivirus. Metabolic dysfunction, lipogenesis and fatty acid-oxidation in liver were evaluated...
  2. Tang J, Wang F, Cheng G, Si S, Sun X, Han J, et al. Wilms' tumor 1-associating protein promotes renal cell carcinoma proliferation by regulating CDK2 mRNA stability. J Exp Clin Cancer Res. 2018;37:40 pubmed publisher
    ..Cells were stably transfected with lentivirus approach and cell proliferation and cell cycle, as well as tumorigenesis in nude mice were performed to assess ..
  3. Wang J, Chen S, Liao Y, Zhang E, Feng S, Yu S, et al. Mitogen-Activated Protein Kinase Kinase 2, a Novel E2-Interacting Protein, Promotes the Growth of Classical Swine Fever Virus via Attenuation of the JAK-STAT Signaling Pathway. J Virol. 2016;90:10271-10283 pubmed publisher
    ..Overexpression of MEK2 significantly promoted the replication of CSFV, whereas knockdown of MEK2 by lentivirus-mediated small hairpin RNAs dramatically inhibited CSFV replication...
  4. Xiong X, Chen D, Sun H, Ding L, Wang J, Chen Q, et al. FNDC5 overexpression and irisin ameliorate glucose/lipid metabolic derangements and enhance lipolysis in obesity. Biochim Biophys Acta. 2015;1852:1867-75 pubmed publisher
    ..In obese mice, lentivirus mediated-FNDC5 overexpression enhanced energy expenditure, lipolysis and insulin sensitivity, and reduced ..
  5. Lei D, Dong C, Wu W, Dong A, Li T, Chan M, et al. Lentiviral Delivery of Small Hairpin RNA Targeting Connective Tissue Growth Factor Blocks Profibrotic Signaling in Tenon's Capsule Fibroblasts. Invest Ophthalmol Vis Sci. 2016;57:5171-5180 pubmed publisher
    ..Knockdown of CTGC was achieved by lentivirus delivery of small-hairpin RNA...
  6. Zhao X, Xu Z, Wang Z, Wu Z, Gong Y, Zhou L, et al. RNA silencing of integrin-linked kinase increases the sensitivity of the A549 lung cancer cell line to cisplatin and promotes its apoptosis. Mol Med Rep. 2015;12:960-6 pubmed publisher
    ..In the present study, ILK downregulation was mediated by lentivirus-mediated RNA interference...
  7. Ghazaryan E, Zhang Y, He Y, Liu X, Li Y, Xie J, et al. Mesenchymal stem cells in corneal neovascularization: Comparison of different application routes. Mol Med Rep. 2016;14:3104-12 pubmed publisher
    ..BMSCs were previously transduced with the green fluorescent protein gene by lentivirus to track the movement of the cells following transplantation...
  8. Okamoto S, Asgar N, Yokota S, Saito K, Minokoshi Y. Role of the α2 subunit of AMP-activated protein kinase and its nuclear localization in mitochondria and energy metabolism-related gene expressions in C2C12 cells. Metabolism. 2019;90:52-68 pubmed publisher
    ..specific for AMPKα1 (shAMPKα1), AMPKα2 (shAMPKα2), or both AMPKα1 and AMPKα2 (shPanAMPK) were generated by lentivirus infection...
  9. Guan G, Li R, Tang W, Liu T, Su Z, Wang Y, et al. Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells. Tumour Biol. 2017;39:1010428317691740 pubmed publisher
    ..Recombinant lentivirus carrying RNA-binding motif 10 complementary DNA was used to infect lung adenocarcinoma cell lines, A549 and ..
  10. Liu H, Hua L, Liu Q, Pan J, Bao Y. Cold Stimuli Facilitate Inflammatory Responses Through Transient Receptor Potential Melastatin 8 (TRPM8) in Primary Airway Epithelial Cells of Asthmatic Mice. Inflammation. 2018;41:1266-1275 pubmed publisher
    ..The TRPM8 overexpression plasmid and siRNA lentivirus were transfected to up- or downregulate the TRPM8 level...
  11. Zhang B, Jiang H, Chen J, Guo X, Hu Q, Yang S. KDM3A inhibition attenuates high concentration insulin?induced vascular smooth muscle cell injury by suppressing MAPK/NF??B pathways. Int J Mol Med. 2018;41:1265-1274 pubmed publisher
    ..b>Lentivirus transduction with KDM3A?siRNA markedly reduced the elevated expression of KDM3A induced by high insulin ..
  12. Jiang X, Huang X, Jiang T, Zheng L, Zhao J, Zhang X. The role of Sox9 in collagen hydrogel-mediated chondrogenic differentiation of adult mesenchymal stem cells (MSCs). Biomater Sci. 2018;6:1556-1568 pubmed publisher
    ..In this paper, MSCs were infected with a vector carrying the Sox9 promoter and related markers were detected. A lentivirus-mediated vector targeting the silencing of the Sox9 gene was used in bone marrow-derived MSCs prior to being ..
  13. Zhang Q, Zhou M, Wu X, Li Z, Liu B, Gao W, et al. Promoting therapeutic angiogenesis of focal cerebral ischemia using thrombospondin-4 (TSP4) gene-modified bone marrow stromal cells (BMSCs) in a rat model. J Transl Med. 2019;17:111 pubmed publisher
    ..We subcloned the tsp4 gene into a lentivirus expression vector system and harvested the tsp4 lentivirus using 293FT cells...
  14. He F, Shi E, Yan L, Li J, Jiang X. Inhibition of micro-ribonucleic acid-320 attenuates neurologic injuries after spinal cord ischemia. J Thorac Cardiovasc Surg. 2015;150:398-406 pubmed publisher
    ..Spinal cord ischemia was induced in rats by cross-clamping the descending aorta for 14 minutes. Lentivirus vectors containing antisense oligonucleotides of rat miR-320 (antagomiR-320) were transfected into the ..
  15. Hu Y, Zhao J, Li S, Huang J, Qiu Y, Ma X, et al. RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction. Arterioscler Thromb Vasc Biol. 2015;35:87-101 pubmed publisher
    ..b>Lentivirus-mediated NFIA overexpression increased high-density lipoprotein cholesterol circulation, reduced low-density ..
  16. Gates K, Goetzmann L, Cantlon J, Jeckel K, Anthony R. Effect of proline rich 15-deficiency on trophoblast viability and survival. PLoS ONE. 2017;12:e0174976 pubmed publisher
    ..b>Lentivirus-mediated knockdown of PRR15 mRNA in ovine trophectoderm led to demise of the embryo by gestational day 15, ..
  17. Hu X, Lei X, Wang J, Pan H, Li C, Yao Z. Lentivirus vector-mediated mitofusin-2 overexpression in rat ovary changes endocrine function and promotes follicular development in vivo. Exp Ther Med. 2014;8:731-736 pubmed
  18. Lin Y, Zhuang J, Li H, Zhu G, Zhou S, Li W, et al. Vaspin attenuates the progression of atherosclerosis by inhibiting ER stress-induced macrophage apoptosis in apoE‑/‑ mice. Mol Med Rep. 2016;13:1509-16 pubmed publisher
    ..Mice were injected with lentivirus via the tail vein and tissues were obtained for histological analysis...
  19. Hu Y, Ye X, Zhou Q, Fu R. Sestrin 2 Attenuates Rat Hepatic Stellate Cell (HSC) Activation and Liver Fibrosis via an mTOR/AMPK-Dependent Mechanism. Cell Physiol Biochem. 2018;51:2111-2122 pubmed publisher
    ..Next, HSC-T6 cells and fibrotic mice were transfected with lentivirus. The mRNA expression levels of markers of liver fibrosis [alpha-smooth muscle actin (α-SMA) and collagen 1A1 (..
  20. Duan M, Yan M, Wang Q, Mao M, Su D, Sun L, et al. Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes. Cell Commun Signal. 2018;16:91 pubmed publisher
    ..The knockdown of miR-1 by hippocampal stereotaxic injection of AMO-1 carried by a lentivirus vector (lenti-pre-AMO-1) led to the upregulation of SNAP-25 expression and prevented SV concentration in the ..
  21. Zhao J, Liu N, Hao X, Que L, Liu J, Tang X. Association between integrin-linked kinase and hyperthermia in oral squamous cell carcinoma. Oncol Lett. 2017;14:7705-7714 pubmed publisher
    ..b>Lentivirus-mediated short hairpin RNA (shRNA)-targeting ILK was transfected into oral squamous cell carcinoma (OSCC) ..
  22. Medina A, Pinilla O, Portiansky E, Caldiz C, Ennis I. Silencing of the Na+/H+ exchanger 1(NHE-1) prevents cardiac structural and functional remodeling induced by angiotensin II. Exp Mol Pathol. 2019;107:1-9 pubmed publisher
    ..The intramyocardial injection of a lentivirus coding a specific small interference RNA (l-shNHE1) significantly reduced NHE-1 expression exclusively in the ..
  23. Ha M, Zhang P, Li L, Liu C. Triclosan Suppresses Testicular Steroidogenesis via the miR-6321/JNK/ Nur77 Cascade. Cell Physiol Biochem. 2018;50:2029-2045 pubmed publisher
    ..were exposed to TCS for 24 h after the pretreatments with the activator of JNK, Nur77 siRNA, or recombinant lentivirus vector for Nur77...
  24. Wang H, Wang J, Qu H, Wei H, Ji B, Yang Z, et al. In vitro and in vivo inhibition of mTOR by 1,25-dihydroxyvitamin D3 to improve early diabetic nephropathy via the DDIT4/TSC2/mTOR pathway. Endocrine. 2016;54:348-359 pubmed
    ..Streptozotocin-induced diabetic rats were injected intravenously with a recombinant lentivirus against the rat vitamin D receptor gene...
  25. Mou K, Liu W, Han D, Li P. HMGB1/RAGE axis promotes autophagy and protects keratinocytes from ultraviolet radiation-induced cell death. J Dermatol Sci. 2017;85:162-169 pubmed publisher
    ..ROS) were determined by H2DCF assay, apoptosis was assayed by flow cytometry and western-blot after lentivirus-mediated shRNA targeting of HMGB1 in keratinocytes by UV irradiation...
  26. Zhai Q, Li F, Chen X, Jia J, Sun S, Zhou D, et al. Triggering Receptor Expressed on Myeloid Cells 2, a Novel Regulator of Immunocyte Phenotypes, Confers Neuroprotection by Relieving Neuroinflammation. Anesthesiology. 2017;127:98-110 pubmed publisher
    ..myeloid cell-specific small interfering RNA or triggering receptor expressed on myeloid cells 2 overexpression lentivirus. Triggering receptor expressed on myeloid cells 2 expression in the ischemic penumbra peaked at 3 days after ..
  27. Zhang W, Wu H, Yang M, Ye S, Li L, Zhang H, et al. SIRT1 inhibition impairs non-homologous end joining DNA damage repair by increasing Ku70 acetylation in chronic myeloid leukemia cells. Oncotarget. 2016;7:13538-50 pubmed publisher
    ..SIRT1 expressions were downregulated by lentivirus-delivered SIRT1 shRNA in myeloid leukemia cells...
  28. Han F, Wang Y, Wang Y, Jia Y, Yang L, Jia W, et al. [EFFECTS OF INDOLEAMINE 2, 3-DIOXYGENASE GENE MODIFIED BONE MARROW MESENCHYMAL STEM CELLS IN RAT COMPOSITE TISSUE ALLOGRAFT REJECTION]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2014;28:745-51 pubmed
    ..isolated from Brown Norway (BN) rats (aged, 4-6 weeks) were infected by IDO [green fluorescent protein (GFP)]-lentivirus. The high expression target gene and biological activity cell line (IDO-BMSCs) were screened...
  29. Luo L, Zheng W, Lian G, Chen H, Li L, Xu C, et al. Combination treatment of adipose-derived stem cells and adiponectin attenuates pulmonary arterial hypertension in rats by inhibiting pulmonary arterial smooth muscle cell proliferation and regulating the AMPK/BMP/Smad pathway. Int J Mol Med. 2018;41:51-60 pubmed publisher
    ..were effectively infected with the green fluorescent protein (GFP)-empty (ADSCs-V) or the APN-GFP (ADSCs-APN) lentivirus and the APN expression was evaluated by ELISA...
  30. Guo T, Song H, Zhao Z, Qi Z, Zhao S. Overexpression of Annexin A2 Receptor Inhibits Neovascularization via the Promotion of Krüppel-Like Transcription Factor 2. Cell Physiol Biochem. 2018;46:1617-1627 pubmed publisher
    ..into human retinal endothelial cells (HRECs) and human umbilical vein endothelial cells (HUVECs) using lentivirus vectors to overexpress AX2R...
  31. Liu F, Wen Y, Kang J, Wei C, Wang M, Zheng Z, et al. Regulation of TLR4 expression mediates the attenuating effect of erythropoietin on inflammation and myocardial fibrosis in rat heart. Int J Mol Med. 2018;42:1436-1444 pubmed publisher
    ..and/or Toll‑like receptor (TLR)4 were overexpressed in rat hearts through intramyocardial administration of lentivirus expressing the EPO and TLR4 genes...
  32. Zheng L, Feng Y, Shi Y, Zhang J, Mu Q, Qin L, et al. Intralipid decreases apolipoprotein M levels and insulin sensitivity in rats. PLoS ONE. 2014;9:e105681 pubmed publisher
    ..Human embryonic kidney 293T cells were transfected with a lentivirus mediated human apoM overexpression system...
  33. Wang Y, He J, Zhang S, Yang Q, Wang B, Liu Z, et al. Knockdown of Immature Colon Carcinoma Transcript 1 Inhibits Proliferation and Promotes Apoptosis of Non-Small Cell Lung Cancer Cells. Technol Cancer Res Treat. 2017;16:559-569 pubmed publisher
    ..b>Lentivirus-mediated RNA interference was used to knock down immature colon carcinoma transcript 1 expression in 95D and ..
  34. Han X, Chen Y, Yao N, Liu H, Wang Z. MicroRNA let-7b suppresses human gastric cancer malignancy by targeting ING1. Cancer Gene Ther. 2015;22:122-9 pubmed publisher
    ..gastric cancer confirmed that let-7b could inhibit gastric cancer metastasis in vivo after transfection by the lentivirus pGCsil-GFP- let-7b...
  35. Han X, Chen M, Hong T, Zhu L, He D, Feng J, et al. Lentivirus-mediated RNAi knockdown of the gap junction protein, Cx43, attenuates the development of vascular restenosis following balloon injury. Int J Mol Med. 2015;35:885-92 pubmed publisher
    ..To determine the involvement of Cx43 in vascular restenosis, the lentivirus vector expressing shRNA targeting Cx43, Cx43-RNAi-LV, was used to silence Cx43 in the rat carotid arteries...
  36. Wang Y, Jiang Y, Ma N, Sang B, Hu X, Cong X, et al. Overexpression of Hiwi Inhibits the Growth and Migration of Chronic Myeloid Leukemia Cells. Cell Biochem Biophys. 2015;73:117-24 pubmed publisher
    ..The present results showed that lentivirus-mediated overexpression of Hiwi significantly suppressed cell proliferation and induced obvious apoptosis in ..
  37. Yu J, Liao X, Li L, Lv L, Zhi X, Yu J, et al. A preliminary study of the role of extracellular -5'- nucleotidase in breast cancer stem cells and epithelial-mesenchymal transition. In Vitro Cell Dev Biol Anim. 2017;53:132-140 pubmed publisher
    ..in various breast cancer cell lines with flow cytometry after overexpression (by lentivirus infection) or suppression (by siRNA interference) of CD73...
  38. Chuanyu S, Yuqing Z, Chong X, Guowei X, Xiaojun Z. Periostin promotes migration and invasion of renal cell carcinoma through the integrin/focal adhesion kinase/c-Jun N-terminal kinase pathway. Tumour Biol. 2017;39:1010428317694549 pubmed publisher
    ..Furthermore, lentivirus-mediated focal adhesion kinase knockdown and c-Jun N-terminal kinase inhibitor reduced POSTN-enhanced ..
  39. Deng W, McLaughlin S, Klinke D. Quantifying spontaneous metastasis in a syngeneic mouse melanoma model using real time PCR. Analyst. 2017;142:2945-2953 pubmed publisher
    ..We first transduced B16 cells with lentivirus expressing firefly luciferase Luc2 gene for bioluminescence imaging...
  40. Kim J, Oliveira V, Yamamoto A, Perlingeiro R. Generation of skeletal myogenic progenitors from human pluripotent stem cells using non-viral delivery of minicircle DNA. Stem Cell Res. 2017;23:87-94 pubmed publisher
    ..generation of functional pluripotent stem cell-derived myogenic progenitors, equivalent to those generated by lentivirus. Our results demonstrate that upon multiple transfections, the minicircle approach allows for the scalable ..
  41. Chen X, Chen J, Xu D, Zhao S, Song H, Peng Y. Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs. BMC Musculoskelet Disord. 2017;18:423 pubmed publisher
    ..Forced OGN expression by OGN-infected lentivirus could increase expression of Wnt5b, RUNX2, OCN, ALP and Colla1, as well as bone formation, while decreases ..
  42. Lamba D, McUsic A, Hirata R, Wang P, Russell D, Reh T. Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells. PLoS ONE. 2010;5:e8763 pubmed publisher
    ..derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences...
  43. Clark A, Safaee M, Oh T, Ivan M, Parimi V, Hashizume R, et al. Stable luciferase expression does not alter immunologic or in vivo growth properties of GL261 murine glioma cells. J Transl Med. 2014;12:345 pubmed publisher
    ..GL261 cells were infected with lentivirus containing a gene encoding firefly luciferase (GL261.luc)...
  44. Wang C, Zhu Z, Liu C, He X, Zhang H, Dong J. Knockdown of yes-associated protein inhibits proliferation and downregulates large tumor suppressor 1 expression in MHCC97H human hepatocellular carcinoma cells. Mol Med Rep. 2015;11:4101-8 pubmed publisher
    ..016). Knockdown of YAP using lentivirus‑small hairpin (sh)RNA significantly inhibited 97H cell growth; in addition, the downregulation of YAP ..
  45. Khalili K, Kaminski R, Gordon J, Cosentino L, Hu W. Genome editing strategies: potential tools for eradicating HIV-1/AIDS. J Neurovirol. 2015;21:310-21 pubmed publisher
    ..Nanoparticle or lentivirus-mediated delivery of next generation Cas9 technologies including nickase or RNA-guided FokI nuclease (RFN) will ..
  46. Zhang Y, Zhang X, Huang J, Dong Q. Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines. Med Oncol. 2015;32:157 pubmed publisher
    ..b>Lentivirus-mediated knockdown of β-catenin expression accelerated cell cycle...
  47. Liu T, Wang S, Wang L, Wang J, Li Y. Targeting CD151 by lentivirus-mediated RNA interference inhibits luminal and basal-like breast cancer cell growth and invasion. Mol Cell Biochem. 2015;407:111-21 pubmed publisher
    ..for proliferation, migration, and invasion of two kinds of cells is diminished after Knockdown of CD151 via lentivirus-mediated CD151 specific shRNA. Tumor cells are arrested in G0/G1 phase. Apoptosis is increased...
  48. Morbach H, Schickel J, Cunningham Rundles C, Conley M, Reisli I, Franco J, et al. CD19 controls Toll-like receptor 9 responses in human B cells. J Allergy Clin Immunol. 2016;137:889-98.e6 pubmed publisher
    ..in CD19-deficient or control B cells and in a B-cell line in which CD19 has been knocked down with lentivirus-transduced short hairpin RNA...
  49. Chen L, Tao Y, Feng J, Jiang Y. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. J Ophthalmol. 2015;2015:186946 pubmed publisher
    ..Moreover, knockdown of apelin in pericyte was achieved via lentivirus-mediated RNA interference...
  50. Yin D, Liang Y, Zheng T, Song R, Wang J, Sun B, et al. EF24 inhibits tumor growth and metastasis via suppressing NF-kappaB dependent pathways in human cholangiocarcinoma. Sci Rep. 2016;6:32167 pubmed publisher
    ..XIAP inhibition by lentivirus mediated RNA interference enhanced EF24-induced apoptosis, while XIAP overexpression reduced it in CCA cells...
  51. Xu W, Lu C, Zhang F, Shao J, Yao S, Zheng S. Dihydroartemisinin counteracts fibrotic portal hypertension via farnesoid X receptor-dependent inhibition of hepatic stellate cell contraction. FEBS J. 2017;284:114-133 pubmed publisher
    ..Experiments on mice using FXR shRNA lentivirus consolidated the results above...
  52. Polinski N, Manfredsson F, Benskey M, Fischer D, Kemp C, Steece Collier K, et al. Impact of age and vector construct on striatal and nigral transgene expression. Mol Ther Methods Clin Dev. 2016;3:16082 pubmed
    ..injections of recombinant adeno-associated virus pseudotypes 2/2 (rAAV2/2), 2/5 (rAAV2/5), 2/9 (rAAV2/9), and lentivirus (LV) expressing green fluorescent protein (GFP) in aged versus young adult rats...
  53. Flerin N, Chen H, Glover T, Lamothe P, Zheng J, Fang J, et al. T-Cell Receptor (TCR) Clonotype-Specific Differences in Inhibitory Activity of HIV-1 Cytotoxic T-Cell Clones Is Not Mediated by TCR Alone. J Virol. 2017;91: pubmed publisher
    ..We show that Jurkat/MA cells and primary CD8+ T cells transduced with lentivirus expressing TCR from one of the ineffective clones exhibited a level of activation by cognate peptide and ..
  54. Yang Z, Xu J, Zhu R, Liu L. Down-Regulation of miRNA-128 Contributes to Neuropathic Pain Following Spinal Cord Injury via Activation of P38. Med Sci Monit. 2017;23:405-411 pubmed
    ..Furthermore, lentivirus production of miR-128 and scrambled control were transfected into BV2 cells, which were first treated with CM-..
  55. Sun L, Zhao M, Zhang J, Lv M, Li Y, Yang X, et al. MiR-29b Downregulation Induces Phenotypic Modulation of Vascular Smooth Muscle Cells: Implication for Intracranial Aneurysm Formation and Progression to Rupture. Cell Physiol Biochem. 2017;41:510-518 pubmed publisher
    ..Next, we modified the level of miR-29b via lentivirus particles in the VSMCs and examined the effects of miR-29b on proliferation, migration, and phenotypic ..
  56. Long Z, Cao M, Su S, Wu G, Meng F, Wu H, et al. Inhibition of hepatocyte nuclear factor 1b induces hepatic steatosis through DPP4/NOX1-mediated regulation of superoxide. Free Radic Biol Med. 2017;113:71-83 pubmed publisher
    ..We found that HNF1b expression was decreased in steatotic livers. We injected mice with lentivirus (LV) expressing HNF1b shRNA to generate mice with hepatic knockdown of HNF1b...
  57. Peng Q, Wang Y, Chen C, Zou Y, Zhang L, Deng S, et al. Inhibiting the CD38/cADPR pathway protected rats against sepsis associated brain injury. Brain Res. 2018;1678:56-63 pubmed publisher
    ..Rats were divided into the sham, CLP group, CLP+ CD38 expression lentivirus (CLP+ CD38 LV), CLP+ CD38 interference lentivirus (CLP+ CD38 Ri), CLP+ negative control lentivirus (CLP+NC) and ..
  58. Zhang Z, Chu S, Wang S, Jiang Y, Gao Y, Yang P, et al. RTP801 is a critical factor in the neurodegeneration process of A53T α-synuclein in a mouse model of Parkinson's disease under chronic restraint stress. Br J Pharmacol. 2018;175:590-605 pubmed publisher
    ..In vivo, stereotaxic injection of shRNA lentivirus was used to confirm our in vitro results...
  59. Jiang Y, Guo Q, Gu J, Xu X, An S, Su F, et al. [Effect of microRNA-29b on proliferation and migration of breast cancer cells and its molecular mechanism]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2017;46:349-356 pubmed
    ..lentiviral expression vector (lenti-miRNA-29b) was constructed and transfected into 293T cells to obtain lentivirus particles that were used to infect breast cancer MCF-7 cells...
  60. Matsunaga W, Ichikawa M, Nakamura A, Ishikawa T, Gotoh A. Lentiviral Vector-mediated Gene Transfer in Human Bladder Cancer Cell Lines. Anticancer Res. 2018;38:2015-2020 pubmed
    ..In this study, we assessed the anti-tumor effect of lentivirus-mediated gene transfection of tumor-suppressor genes in human bladder cancer cells...
  61. Li S, Yang M, Chen Y, Tang X, Zhang S, Ni S, et al. Dendritic cells with increased expression of suppressor of cytokine signaling 1(SOCS1) gene ameliorate lipopolysaccharide/d-galactosamine-induced acute liver failure. Mol Immunol. 2018;101:10-18 pubmed publisher
    ..Bone marrow derived dendritic cells were transfected with lentivirus encoding SOCS1 and negative control lentivirus, thereafter collected for costimulatory molecules analysis, ..
  62. Brown M, Munkhtsog B, Troyer J, Ross S, Sellers R, Fine A, et al. Feline immunodeficiency virus (FIV) in wild Pallas' cats. Vet Immunol Immunopathol. 2010;134:90-5 pubmed publisher
    Feline immunodeficiency virus (FIV), a feline lentivirus related to HIV, causes immune dysfunction in domestic and wild cats...
  63. Tian H, Lu J, Shao C, Huffman K, Carstens R, Larsen J, et al. Systematic siRNA Screen Unmasks NSCLC Growth Dependence by Palmitoyltransferase DHHC5. Mol Cancer Res. 2015;13:784-94 pubmed publisher
    ..Silencing of DHHC5 by lentivirus-mediated expression of DHHC5 shRNAs dramatically reduced in vitro cell proliferation, colony formation, and cell ..
  64. Broggini T, Czabanka M, Piffko A, Harms C, Hoffmann C, Mrowka R, et al. ICAM1 depletion reduces spinal metastasis formation in vivo and improves neurological outcome. Eur Spine J. 2015;24:2173-81 pubmed publisher
    ..B16 melanoma cells were infected with a lentivirus containing firefly luciferase (B16-luc)...
  65. Zhang Q, Guo Y, Dong R, Dai R, Zhou M. Suppressor of cytokine signaling 1-modulated metalloproteinases and tissue inhibitor of metalloproteinase in pulmonary fibrosis. Mol Med Rep. 2015;12:3855-3861 pubmed publisher
    ..growth factor??1 (TGF??1) in conditions of deficiency and over?expression of SOCS1, by transfection with a lentivirus. Overexpression of SOCS1 in A549 cells and HLFs significantly inhibited the mRNA expression levels of MMP?1, MMP?..
  66. Hjelm B, Grunseich C, Gowing G, Avalos P, Tian J, Shelley B, et al. Mifepristone-inducible transgene expression in neural progenitor cells in vitro and in vivo. Gene Ther. 2016;23:424-37 pubmed publisher
    ..Our inducible system used a lentivirus-based vector platform for the ex vivo production of mifepristone-inducible murine neural progenitor cells that ..
  67. Kennedy A, Cribbs A. Production and Concentration of Lentivirus for Transduction of Primary Human T Cells. Methods Mol Biol. 2016;1448:85-93 pubmed publisher
    ..We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human ..
  68. Wang L, Sun Y, Yi J, Wang X, Liang J, Pan Z, et al. Targeting H19 by lentivirus-mediated RNA interference increases A549 cell migration and invasion. Exp Lung Res. 2016;42:346-353 pubmed
    ..But its role in tumor metastasis is still unclear. MACC1 RNAi and Lentivirus-mediated H19-specific shRNA was used to establish H19 stable knocking-down A549 cells...
  69. Teimoori S, Seesuay W, Jittavisutthikul S, Chaisri U, Sookrung N, Densumite J, et al. Human transbodies to VP40 inhibit cellular egress of Ebola virus-like particles. Biochem Biophys Res Commun. 2016;479:245-252 pubmed publisher
    ..inhibited egress of the Ebola virus-like particles from human hepatic cells transduced with pseudo-typed-Lentivirus particles carrying EBOV VP40 and GP genes...
  70. Liu Y, Lv H, Wu X, Zhou J, Shi Y, Wen J. Demethylation of Repressor Element-1 Silencing Transcription (REST) Suppresses the Malignant Phenotype of Breast Cancer via MMP9. Oncol Res. 2017;25:445-454 pubmed publisher
    ..Additionally, we elucidated the functions of REST on proliferation and invasion in breast cancer cells. Lentivirus transfection was used to overexpress REST in human breast MDA-MB-231 cells...
  71. Zhang S, Zhou Y, Zhang K, Luan J, Qi S. [Neuroprotective effect of Nogo-66 receptor silencing in preterm rats with brain injury caused by intrauterine infection]. Zhongguo Dang Dai Er Ke Za Zhi. 2016;18:1035-1043 pubmed
    ..those in the empty vector and NgR-siRNA groups were given an injection of lentiviral empty vector or NgR-siRNA lentivirus via the lateral ventricle on postnatal day 1 (P1) and then fed routinely...
  72. Yan L, Xie M, Lu H, Zhang H, Shi M, Zhang Y, et al. Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor. Mol Neurobiol. 2018;55:495-505 pubmed publisher
    ..Neuritin silencing or overexpressing lentivirus transfection of Schwann cells was conducted...
  73. Li J, Ma F, Wang Y, Chen F, Lu S, Chi Y, et al. Knockdown of IL-8 Provoked Premature Senescence of Placenta-Derived Mesenchymal Stem Cells. Stem Cells Dev. 2017;26:912-931 pubmed publisher
    ..We then employed lentivirus-mediated small hairpin RNA interference to generate stable IL-8-silenced PMSCs, which displayed a variety of ..
  74. Wang J, Xu W, Shao J, He Z, Ding Z, Huang J, et al. miR-219-5p targets CaMKIIγ to attenuate morphine tolerance in rats. Oncotarget. 2017;8:28203-28214 pubmed publisher
    ..Overexpression of miR-219-5p by lentivirus injection prevents the development of morphine tolerance...
  75. Periyasamy P, Liao K, Kook Y, Niu F, Callen S, Guo M, et al. Cocaine-Mediated Downregulation of miR-124 Activates Microglia by Targeting KLF4 and TLR4 Signaling. Mol Neurobiol. 2018;55:3196-3210 pubmed publisher
    ..Intriguingly, stereotactic administration of lentivirus-miR-124 in the striatum significantly inhibited cocaine-mediated microglial activation and locomotor ..
  76. Fu M, Wang C, Hsieh Y, Fang K, Tzeng S. Functional Role of Matrix gla Protein in Glioma Cell Migration. Mol Neurobiol. 2018;55:4624-4636 pubmed publisher
    ..Interestingly, C6-2 cells expressed higher levels of MGP molecules than C6-1 cells did. Lentivirus-mediated short hairpin RNA (shRNA) against MGP gene expression (MGP-KD) in C6-2 cells or lentivirus-mediated ..
  77. Wang Z, Dai Z, Pan Y, Wu S, Li Z, Zuo C. E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line. Biochem Biophys Res Commun. 2017;492:419-424 pubmed publisher
    ..While DTX4 stable knockdown by recombinant shRNA lentivirus (sh-DTX4) notably reduced the number of lipid droplets and down-regulated the expression of adipogenic ..
  78. Unno K, Roh M, Yoo Y, Al Shraideh Y, Wang L, Nonn L, et al. Modeling African American prostate adenocarcinoma by inducing defined genetic alterations in organoids. Oncotarget. 2017;8:51264-51276 pubmed publisher
    ..rates and demonstrated pathologically transformed morphology compared to organoids transduced with control lentivirus. Alterations in MYC, PTEN and TP53 also affected the rate of organoid basal-to-luminal differentiation in ..
  79. Chen W, Zhang B, Xu S, Lin R, Wang W. Lentivirus carrying the NeuroD1 gene promotes the conversion from glial cells into neurons in a spinal cord injury model. Brain Res Bull. 2017;135:143-148 pubmed publisher
    ..Group A received only normal saline injection. Group B received unloaded lentivirus injection. Group C received NeuroD1 recombinant lentivirus injection...
  80. Lei W, Li Q, Su S, Bu J, Huang Q, Li Z. Chlamydia trachomatis plasmid-encoded protein pORF5 protects mitochondrial function by inducing mitophagy and increasing HMGB1 expression. Pathog Dis. 2017;75: pubmed publisher
    ..Knockdown high mobility group box 1 (HMGB1) by lentivirus suppressed pORF5-induced mitophagy and increased apoptosis, implying that pORF5 may participate in cell death ..
  81. Bangham C. Human T Cell Leukemia Virus Type 1: Persistence and Pathogenesis. Annu Rev Immunol. 2018;36:43-71 pubmed publisher
    ..Unlike the distantly related lentivirus HIV-1, HTLV-1 causes disease in only 5-10% of infected people, depending on their ethnic origin...
  82. Liu L, Jin X, Hu C, Zhang Y, Zhou Z, Li R, et al. Amphiregulin enhances cardiac fibrosis and aggravates cardiac dysfunction in mice with experimental myocardial infarction partly through activating EGFR-dependent pathway. Basic Res Cardiol. 2018;113:12 pubmed publisher
    ..To verify the functions of AR in MI hearts, lentivirus-AR-shRNA and negative control vectors were delivered into the infarct border zone...
  83. Kim C, Hong Y, Lee H, Kang H, Lee E. MicroRNA-195 desensitizes HCT116 human colon cancer cells to 5-fluorouracil. Cancer Lett. 2018;412:264-271 pubmed publisher
    ..Human colorectal cancer HCT-116 cell lines were transduced with a lentivirus library containing 578 precursor microRNAs (miRNAs) to establish cell lines resistant to 5-fluorouracil (5-FU)...
  84. Zhang H, Wang Z, Feng S, Xu L, Shi H, Chen L, et al. PEDF improves cardiac function in rats with acute myocardial infarction via inhibiting vascular permeability and cardiomyocyte apoptosis. Int J Mol Sci. 2015;16:5618-34 pubmed publisher
    ..PEDF-small interfering RNA (siRNA)-lentivirus (PEDF-RNAi-LV) or PEDF-LV was delivered into the myocardium along the infarct border to knockdown or overexpress ..
  85. Gu M, Zhang K, Yao H, Zhou J, Peng Y, Xu M, et al. RNAi-mediated knockdown of vascular endothelial growth factor inhibits vascularization and tumor growth in renal cell carcinoma. Int J Mol Med. 2015;36:1063-72 pubmed publisher
    ..05). Compared with the controls, injections of lentivirus expressing VEGF-shRNA significantly inhibited tumor growth, and reduced tumor mass and VEGF expression in the ..
  86. Zhou C, Wang C, Yu A, Wang Q, Zhan M, Tang J, et al. MiR-630 suppresses breast cancer progression by targeting metadherin. Oncotarget. 2016;7:1288-99 pubmed publisher
    ..Moreover, lentivirus delivered miR-630 bestows MDA-MB-231-luc cells with the ability to suppress cell colony formation in vitro and ..
  87. Wang Y, Feng G, Xia Q, Hu Y, Xu Y, Xiong L, et al. Knockdown of α-synuclein in cerebral cortex improves neural behavior associated with apoptotic inhibition and neurotrophin expression in spinal cord transected rats. Apoptosis. 2016;21:404-20 pubmed publisher
    ..In order to detect the role of SNCA, we used SNCA-ORF/shRNA lentivirus to upregulate or knockdown SNCA expression...
  88. Fu Q, Shi D, Zhou Y, Zheng H, Xiang H, Tian X, et al. MHC-I promotes apoptosis of GABAergic interneurons in the spinal dorsal horn and contributes to cancer induced bone pain. Exp Neurol. 2016;286:12-20 pubmed publisher
    ..Pretreatment of MHC-I RNAi-lentivirus could prevent the apoptosis of GABAergic interneurons and therefore alleviated mechanical allodynia induced by ..
  89. Chung S, Weber F, Zhong P, Tan C, Nguyen T, BEIER K, et al. Identification of preoptic sleep neurons using retrograde labelling and gene profiling. Nature. 2017;545:477-481 pubmed publisher
    ..Using a lentivirus expressing channelrhodopsin-2 or a light-activated chloride channel for retrograde labelling, bidirectional ..
  90. Tang X, Lin J, Wang G, Lu J. MicroRNA-433-3p promotes osteoblast differentiation through targeting DKK1 expression. PLoS ONE. 2017;12:e0179860 pubmed publisher
    ..Rat bone marrow derived osteoblasts were infected with lentivirus vector in which miR-433-3p was constructed...
  91. Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K. Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail. PLoS ONE. 2015;10:e0124768 pubmed publisher
    ..containing a green fluorescent protein (GFP) gene under the control of the RBP7 promoter were generated using lentivirus-mediated gene transfer...
  92. Zhang H, Sun T, Jiang X, Yu H, Wang M, Wei T, et al. PEDF and PEDF-derived peptide 44mer stimulate cardiac triglyceride degradation via ATGL. J Transl Med. 2015;13:68 pubmed publisher
    ..The left ascending coronary artery (LAD) was ligated to induce AMI. PEDF-small interfering RNA (siRNA)-lentivirus (PEDF-RNAi-LV) or PEDF-LV was delivered to the ischemic myocardium in order to knock down or overexpress PEDF, ..