Dengue virus 2

Summary

Alias: Dengue virus type 2, dengue 2 virus DEN-2, dengue-2 virus, dengue-2 virus DEN-2, Dengue virus type II

Top Publications

  1. Li H, Clum S, You S, Ebner K, Padmanabhan R. The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids. J Virol. 1999;73:3108-16 pubmed
    NS3 protein of dengue virus type 2 has a serine protease domain within the N-terminal 180 residues. NS2B is required for NS3 to form an active protease involved in processing of the viral polyprotein precursor...
  2. Johansson M, Brooks A, Jans D, Vasudevan S. A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-beta and the viral helicase, NS3. J Gen Virol. 2001;82:735-45 pubmed
    ..The direct interaction of importin-beta with NS5 has implications for the mechanism by which this normally cytoplasmic protein may be targetted to the nucleus. ..
  3. Kanai R, Kar K, Anthony K, Gould L, Ledizet M, Fikrig E, et al. Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes. J Virol. 2006;80:11000-8 pubmed
    ..By revealing the details of the molecular landscape of the West Nile virus surface, our structure will assist the design of antiviral vaccines and therapeutics. ..
  4. Schlesinger J, Brandriss M, Walsh E. Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. J Gen Virol. 1987;68 ( Pt 3):853-7 pubmed
    Immunization of mice with the dengue 2 virus (DEN 2)-specified non-structural protein NS1 provided significant protection against intracerebral challenge with the virus in the absence of detectable neutralizing or other anti-virion ..
  5. Sánchez I, Ruiz B. A single nucleotide change in the E protein gene of dengue virus 2 Mexican strain affects neurovirulence in mice. J Gen Virol. 1996;77 ( Pt 10):2541-5 pubmed
    Eleven clones of dengue virus 2 Mexican strain were selected by the size of their lytic plaques. Nucleotide sequences of the clones producing large plaques (D2ML2, D2ML3 and D2ML4) revealed 11 mutations, 10 of which were silent...
  6. Falconar A. Identification of an epitope on the dengue virus membrane (M) protein defined by cross-protective monoclonal antibodies: design of an improved epitope sequence based on common determinants present in both envelope (E and M) proteins. Arch Virol. 1999;144:2313-30 pubmed
    ..These peptide sequence will now be tested for their ability to generate cross-protective antibodies against each dengue virus serotype when delivered with appropriate T-helper epitopes. ..
  7. Kuhn R, Zhang W, Rossmann M, Pletnev S, Corver J, Lenches E, et al. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell. 2002;108:717-25 pubmed
    ..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane. ..
  8. Bartelma G, Padmanabhan R. Expression, purification, and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 nonstructural protein 3. Virology. 2002;299:122-32 pubmed
    b>Dengue virus type 2 (DEN2), a member of the Flaviviridae family of positive-strand RNA viruses, contains a single RNA genome having a type I cap structure at the 5' end...
  9. Yap T, Xu T, Chen Y, Malet H, Egloff M, Canard B, et al. Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution. J Virol. 2007;81:4753-65 pubmed
    ..In the absence of a template strand, a chain-terminating nucleoside analogue binds to the priming loop site. These results should inform and accelerate the structure-based design of antiviral compounds against dengue virus. ..

More Information

Publications121 found, 100 shown here

  1. Egloff M, Benarroch D, Selisko B, Romette J, Canard B. An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. EMBO J. 2002;21:2757-68 pubmed
    ..The crystal structure of an N-terminal fragment of Dengue virus type 2 NS5 is reported at 2.4 A resolution...
  2. Tassaneetrithep B, Burgess T, Granelli Piperno A, Trumpfheller C, Finke J, Sun W, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med. 2003;197:823-9 pubmed
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection. ..
  3. Modis Y, Ogata S, Clements D, Harrison S. A ligand-binding pocket in the dengue virus envelope glycoprotein. Proc Natl Acad Sci U S A. 2003;100:6986-91 pubmed
    ..A crystal structure of the soluble ectodomain of E from dengue virus type 2 reveals a hydrophobic pocket lined by residues that influence the pH threshold for fusion...
  4. Zhang W, Chipman P, Corver J, Johnson P, Zhang Y, Mukhopadhyay S, et al. Visualization of membrane protein domains by cryo-electron microscopy of dengue virus. Nat Struct Biol. 2003;10:907-12 pubmed
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus. ..
  5. Modis Y, Ogata S, Clements D, Harrison S. Structure of the dengue virus envelope protein after membrane fusion. Nature. 2004;427:313-9 pubmed
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry. ..
  6. Pryor M, Azzola L, Wright P, Davidson A. Histidine 39 in the dengue virus type 2 M protein has an important role in virus assembly. J Gen Virol. 2004;85:3627-36 pubmed
    ..In this study, the role of the His residue at position 39 in the M protein (M39) of dengue virus type 2 (DENV-2) in the virus life cycle was investigated...
  7. Sakoonwatanyoo P, Boonsanay V, Smith D. Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein. Intervirology. 2006;49:161-72 pubmed
    ..The results suggest that multiple receptors may be used by the dengue virus to enter into insect cells, and that one of these proteins may be a laminin-binding protein. ..
  8. Yu I, Zhang W, Holdaway H, Li L, Kostyuchenko V, Chipman P, et al. Structure of the immature dengue virus at low pH primes proteolytic maturation. Science. 2008;319:1834-7 pubmed publisher
    ..These results suggest a mechanism by which flaviviruses are processed and stabilized in the host cell secretory pathway. ..
  9. Bielefeldt Ohmann H. Analysis of antibody-independent binding of dengue viruses and dengue virus envelope protein to human myelomonocytic cells and B lymphocytes. Virus Res. 1998;57:63-79 pubmed
  10. Leitmeyer K, Vaughn D, Watts D, Salas R, Villalobos I, de Chacon -, et al. Dengue virus structural differences that correlate with pathogenesis. J Virol. 1999;73:4738-47 pubmed
    ..This new approach to the study of dengue virus genome differences should better reflect the true composition of viral RNA populations in the natural host and permit their association with pathogenesis. ..
  11. Filomatori C, Iglesias N, Villordo S, Alvarez D, Gamarnik A. RNA sequences and structures required for the recruitment and activity of the dengue virus polymerase. J Biol Chem. 2011;286:6929-39 pubmed publisher
    ..We propose that the long range RNA-RNA interactions in the viral genome play multiple roles during RNA synthesis. Together, we provide new molecular details about the promoter-dependent dengue virus RNA polymerase activity. ..
  12. Kinney R, Butrapet S, Chang G, Tsuchiya K, Roehrig J, Bhamarapravati N, et al. Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. Virology. 1997;230:300-8 pubmed
    ..Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice. ..
  13. Hung S, Lee P, Chen H, Chen L, Kao C, King C. Analysis of the steps involved in Dengue virus entry into host cells. Virology. 1999;257:156-67 pubmed
    ..We found that three MAbs, 17-2, 46-9, and 51-3, may neutralize dengue 2 virus (DEN-2) through inhibition of not only viral attachment but also of penetration. However, one MAb, 56-3...
  14. Nomaguchi M, Ackermann M, Yon C, You S, Padmanabhan R, Padmanbhan R. De novo synthesis of negative-strand RNA by Dengue virus RNA-dependent RNA polymerase in vitro: nucleotide, primer, and template parameters. J Virol. 2003;77:8831-42 pubmed
  15. Xu T, Sampath A, Chao A, Wen D, Nanao M, Chene P, et al. Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A. J Virol. 2005;79:10278-88 pubmed
    ..A possible model for the unwinding mechanism is proposed. ..
  16. Ackermann M, Padmanabhan R. De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase. J Biol Chem. 2001;276:39926-37 pubmed
    ..A model is proposed that a specific conformation of the viral polymerase and/or structure at the 3' end of the template RNA is required for de novo initiation. ..
  17. Chiu M, Yang Y. Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli. Biochem Biophys Res Commun. 2003;309:672-8 pubmed
  18. Selisko B, Dutartre H, Guillemot J, Debarnot C, Benarroch D, Khromykh A, et al. Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases. Virology. 2006;351:145-58 pubmed publisher
    ..This suggests that they display a higher conformational flexibility upon the transition from initiation to elongation...
  19. Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol. 2005;3:13-22 pubmed publisher
    ..This review focuses mainly on structural studies of dengue virus...
  20. Smith K, Nanda K, Spears C, Ribeiro M, Vancini R, Piper A, et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J. 2011;8:289 pubmed publisher
    ..This method for creating live, attenuated viral mutants that generate safe and effective immunity may be applied to many other insect-borne viral diseases for which no current effective therapies exist...
  21. Puttikhunt C, Kasinrerk W, Srisa ad S, Duangchinda T, Silakate W, Moonsom S, et al. Production of anti-dengue NS1 monoclonal antibodies by DNA immunization. J Virol Methods. 2003;109:55-61 pubmed
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  22. Alcon LePoder S, Drouet M, Roux P, Frenkiel M, Arborio M, Durand Schneider A, et al. The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity. J Virol. 2005;79:11403-11 pubmed publisher
  23. Mercado Curiel R, Esquinca Avil s H, Tovar R, D az Badillo A, Camacho Nuez M. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006;6:85 pubmed publisher
    ..aegypti midgut. Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells...
  24. Chung K, Liszewski M, Nybakken G, Davis A, Townsend R, Fremont D, et al. West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H. Proc Natl Acad Sci U S A. 2006;103:19111-6 pubmed publisher
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  25. MacKenzie J, Jones M, Young P. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 1996;220:232-40 pubmed publisher
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  26. Westaway E, Khromykh A, Kenney M, MacKenzie J, Jones M. Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus. Virology. 1997;234:31-41 pubmed publisher
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  27. Wang S, He R, Anderson R. PrM- and cell-binding domains of the dengue virus E protein. J Virol. 1999;73:2547-51 pubmed
  28. Flamand M, Megret F, Mathieu M, Lepault J, Rey F, Deubel V. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol. 1999;73:6104-10 pubmed
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  29. Libraty D, Young P, Pickering D, Endy T, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis. 2002;186:1165-8 pubmed publisher
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  30. Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan K, et al. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis. 2006;193:1078-88 pubmed publisher
    ..Complement activation mediated by NS1 leads to local and systemic generation of anaphylatoxins and SC5b-9, which may contribute to the pathogenesis of the vascular leakage that occurs in patients with DHF/DSS...
  31. Cleaves G, Ryan T, Schlesinger R. Identification and characterization of type 2 dengue virus replicative intermediate and replicative form RNAs. Virology. 1981;111:73-83 pubmed
  32. Medin C, Fitzgerald K, Rothman A. Dengue virus nonstructural protein NS5 induces interleukin-8 transcription and secretion. J Virol. 2005;79:11053-61 pubmed publisher
    ..To establish in vitro models for chemokine induction by dengue 2 virus (DEN2V), we studied a variety of human cell lines and primary cells...
  33. Lin C, Chiu S, Hsiao Y, Wan S, Lei H, Shiau A, et al. Expression of cytokine, chemokine, and adhesion molecules during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1. J Immunol. 2005;174:395-403 pubmed
    ..These results suggest the involvement of anti-DV NS1 Abs in the vasculopathy of DV infection...
  34. Chua J, Bhuvanakantham R, Chow V, Ng M. Recombinant non-structural 1 (NS1) protein of dengue-2 virus interacts with human STAT3beta protein. Virus Res. 2005;112:85-94 pubmed publisher
    ..Since the STAT3beta protein is an acute-phase response factor, its interaction with NS1 protein may influence the pathological changes observed in dengue fever, dengue hemorrhagic fever and dengue shock syndrome...
  35. Fajardo Sánchez E, Galiano V, Villalaín J. Spontaneous membrane insertion of a dengue virus NS2A peptide. Arch Biochem Biophys. 2017;627:56-66 pubmed publisher
    ..These data should help us in the development of inhibitor molecules that target NS2A segments involved in membrane reorganisation. ..
  36. Preugschat F, Yao C, Strauss J. In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3. J Virol. 1990;64:4364-74 pubmed
    ..The data from these expression experiments confirm that NS3 is the viral proteinase responsible for cleavage events generating the amino termini of NS2B and NS3 and presumably for cleavages generating the termini of NS4A and NS5 as well...
  37. Fibriansah G, Ibarra K, Ng T, Smith S, Tan J, Lim X, et al. DENGUE VIRUS. Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers. Science. 2015;349:88-91 pubmed publisher
    ..HMAb 2D22 "locks" two-thirds of or all dimers on the virus surface, depending on the strain, but neutralizes these DENV2 strains with equal potency. The epitope defined by HMAb 2D22 is a potential target for vaccines and therapeutics. ..
  38. Murugan K, Aruna P, Panneerselvam C, Madhiyazhagan P, Paulpandi M, Subramaniam J, et al. Fighting arboviral diseases: low toxicity on mammalian cells, dengue growth inhibition (in vitro), and mosquitocidal activity of Centroceras clavulatum-synthesized silver nanoparticles. Parasitol Res. 2016;115:651-62 pubmed
    ..aegypti, with little cytotoxicity on mammalian cells. ..
  39. Miller S, Sparacio S, Bartenschlager R. Subcellular localization and membrane topology of the Dengue virus type 2 Non-structural protein 4B. J Biol Chem. 2006;281:8854-63 pubmed publisher
    ..This topology model provides a good starting point for a detailed investigation of the function of NS4B in the DV life cycle...
  40. Basu A, Jain P, Sarkar P, Gangodkar S, Deshpande D, Ganti K, et al. Dengue virus infection of SK Hep1 cells: inhibition of in vitro angiogenesis and altered cytomorphology by expressed viral envelope glycoprotein. FEMS Immunol Med Microbiol. 2011;62:140-7 pubmed publisher
  41. Naik N, Wu H. Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication. J Virol. 2015;89:6746-60 pubmed publisher
    ..This novel finding may have broader implications in flaviviral biology as the most likely glycan at Asn-62 of NS4B is conserved in DENV serotypes and in some related flaviviruses. ..
  42. Falgout B, Pethel M, Zhang Y, Lai C. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol. 1991;65:2467-75 pubmed
    ..Indirect evidence that NS3 can also act in trans was obtained. Models are discussed for a two-component protease activity requiring both NS2B and NS3...
  43. Hiramatsu K, Tadano M, Men R, Lai C. Mutational analysis of a neutralization epitope on the dengue type 2 virus (DEN2) envelope protein: monoclonal antibody resistant DEN2/DEN4 chimeras exhibit reduced mouse neurovirulence. Virology. 1996;224:437-45 pubmed publisher
  44. Skehel J, Wiley D. Coiled coils in both intracellular vesicle and viral membrane fusion. Cell. 1998;95:871-4 pubmed
  45. Patkar C, Kuhn R. Development of novel antivirals against flaviviruses. Novartis Found Symp. 2006;277:41-52; discussion 52-6, 71-3, 251-3 pubmed
  46. Wu R, Tsai M, Tsai K, Tian J, Wu J, Wu S, et al. Mutagenesis of Dengue Virus Protein NS2A Revealed a Novel Domain Responsible for Virus-Induced Cytopathic Effect and Interactions between NS2A and NS2B Transmembrane Segments. J Virol. 2017;91: pubmed publisher
    ..These results expand the understanding of flavivirus replication. The knowledge may also facilitate studies of pathogenesis and novel vaccine and antiflaviviral drug development. ..
  47. Slon Campos J, Poggianella M, Marchese S, Bestagno M, Burrone O. Secretion of dengue virus envelope protein ectodomain from mammalian cells is dependent on domain II serotype and affects the immune response upon DNA vaccination. J Gen Virol. 2015;96:3265-79 pubmed publisher
  48. White S, Iovine N, Nickels L, Morris J, Lednicky J. Complete Genome Sequence of Dengue virus Type 2 from a Resident of North-Central Florida with Locally Transmitted Dengue Fever. Genome Announc. 2017;5: pubmed publisher
    The majority of dengue fever cases reported in the United States recently have been imported. We isolated dengue virus type 2 (DENV-2) from a North-Central Florida resident with locally acquired dengue fever in May 2016...
  49. Forwood J, Brooks A, Briggs L, Xiao C, Jans D, Vasudevan S. The 37-amino-acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site. Biochem Biophys Res Commun. 1999;257:731-7 pubmed publisher
    ..The linker may have an important role in targeting NS5 to the nucleus in a regulated manner during the dengue virus infectious cycle...
  50. Yusof R, Clum S, Wetzel M, Murthy H, Padmanabhan R. Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro. J Biol Chem. 2000;275:9963-9 pubmed
    b>Dengue virus type 2 NS3, a multifunctional protein, has a serine protease domain (NS3pro) that requires the conserved hydrophilic domain of NS2B for protease activity in cleavage of the polyprotein precursor at sites following two basic ..
  51. Bera A, Kuhn R, Smith J. Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease. J Biol Chem. 2007;282:12883-92 pubmed publisher
    ..Formation of an active protease was found to require neither cleavage of NS2B from NS3 nor a free NS3 N terminus. Evidence was also obtained for product inhibition of the protease by the cleaved C terminus of NS2B...
  52. Butrapet S, Childers T, Moss K, Erb S, Luy B, Calvert A, et al. Amino acid changes within the E protein hinge region that affect dengue virus type 2 infectivity and fusion. Virology. 2011;413:118-27 pubmed publisher
    ..Possible interactions between hinge residues 52 and 277, or among 53, 135, 170, 186, 265, and 276 required for hinge function were discovered by sequence analysis to identify compensatory mutations...
  53. Cheng Y, Lee J, Lo I, Chen S, Hu H, Wu Y, et al. Ecdysones from Zoanthus spp. with inhibitory activity against dengue virus 2. Bioorg Med Chem Lett. 2016;26:2344-8 pubmed publisher
    ..The in vitro antiviral activities of all isolated ecdysones (1-14) against dengue virus type 2 (DENV-2) were evaluated using DENV infectious system...
  54. Crill W, Roehrig J. Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells. J Virol. 2001;75:7769-73 pubmed publisher
    ..These results extend our understanding of the structure-function relationships in the E glycoprotein of DEN virus and provide the first direct evidence that domain III encodes the primary flavivirus receptor-binding motif...
  55. Jones C, Ma L, Burgner J, Groesch T, Post C, Kuhn R. Flavivirus capsid is a dimeric alpha-helical protein. J Virol. 2003;77:7143-9 pubmed
    ..In contrast, the N terminus was found to be unstructured and could be removed without disrupting the structural integrity of the protein...
  56. Leyssen P, Balzarini J, De Clercq E, Neyts J. The predominant mechanism by which ribavirin exerts its antiviral activity in vitro against flaviviruses and paramyxoviruses is mediated by inhibition of IMP dehydrogenase. J Virol. 2005;79:1943-7 pubmed publisher
    ..These data provide compelling evidence that the predominant mechanism of action of ribavirin in vitro against flavi- and paramyxoviruses is based on inhibition of cellular IMP dehydrogenase activity...
  57. Shiryaev S, Ratnikov B, Chekanov A, Sikora S, Rozanov D, Godzik A, et al. Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase. Biochem J. 2006;393:503-11 pubmed publisher
    ..Overall, our findings represent a foundation for in-depth mechanistic and structural studies as well as for the design of novel and efficient inhibitors of WNV NS3...
  58. Lisova O, Hardy F, Petit V, Bedouelle H. Mapping to completeness and transplantation of a group-specific, discontinuous, neutralizing epitope in the envelope protein of dengue virus. J Gen Virol. 2007;88:2387-97 pubmed publisher
    ..The epitope straddles residues of ED3 that are involved in virulence, e.g. Asn/Asp390. These results define the epitope of mAb4E11 as an antigenic signature of the DENV group and suggest mechanisms for its neutralization potency...
  59. Junjhon J, Lausumpao M, Supasa S, Noisakran S, Songjaeng A, Saraithong P, et al. Differential modulation of prM cleavage, extracellular particle distribution, and virus infectivity by conserved residues at nonfurin consensus positions of the dengue virus pr-M junction. J Virol. 2008;82:10776-91 pubmed publisher
  60. Lin C, Wang Y, Hung E, Yang Y, Lee J, Sheu J, et al. Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication. Planta Med. 2017;83:158-163 pubmed publisher
    ..Compounds 3, 4, 5, and 6 exhibited a significant reduction effect on dengue virus type 2 replication with EC50 values of 21.2, 16.5, 12.3, and 11.2?µM, respectively.
  61. Toro J, Salgado D, Vega R, Rodríguez J, Rodríguez L, Angel J, et al. Total and Envelope Protein-Specific Antibody-Secreting Cell Response in Pediatric Dengue Is Highly Modulated by Age and Subsequent Infections. PLoS ONE. 2016;11:e0161795 pubmed publisher
    ..These results are important in the search for correlates of protection and for determining the ideal age for vaccinating against dengue. ..
  62. Arias C, Preugschat F, Strauss J. Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain. Virology. 1993;193:888-99 pubmed publisher
    ..The importance of this alternatively processed form of NS3 and its role in the replication cycle of dengue virus remain to be determined...
  63. Zhang Y, Zhang W, Ogata S, Clements D, Strauss J, Baker T, et al. Conformational changes of the flavivirus E glycoprotein. Structure. 2004;12:1607-18 pubmed publisher
    ..Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses...
  64. Mueller N, Yon C, Ganesh V, Padmanabhan R. Characterization of the West Nile virus protease substrate specificity and inhibitors. Int J Biochem Cell Biol. 2007;39:606-14 pubmed publisher
    ..Furthermore, we show that the WNV and the dengue virus type 2 (DENV-2) proteases are inhibited by aprotinin with inhibitor constants of 0.16 and 0...
  65. Sun D, King C, Huang H, Shih Y, Lee C, Tsai W, et al. Antiplatelet autoantibodies elicited by dengue virus non-structural protein 1 cause thrombocytopenia and mortality in mice. J Thromb Haemost. 2007;5:2291-9 pubmed publisher
    ..These results suggest that the platelet-bound antibody fractions of anti-NS1 Ig are prothrombotic, which might exacerbate the severity of disease in hosts with an imbalanced coagulant system...
  66. Watterson D, Kobe B, Young P. Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding. J Gen Virol. 2012;93:72-82 pubmed publisher
    ..These studies have also demonstrated differential binding between mosquito and human cells...
  67. Eyre N, Johnson S, Eltahla A, Aloi M, Aloia A, McDevitt C, et al. Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses. J Virol. 2017;91: pubmed publisher
    ..Together, this genetic profile of DENV may be further refined and exploited in the identification of antiviral targets and the generation of reporter virus tools. ..
  68. Kapoor M, Zhang L, Ramachandra M, Kusukawa J, Ebner K, Padmanabhan R. Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J Biol Chem. 1995;270:19100-6 pubmed
    b>Dengue virus type 2, a member of the family Flaviviridae, encodes a single polyprotein precursor consisting of 3391 amino acids residues that is processed to at least 10 mature proteins by host and viral proteases...
  69. Diamond M, Harris E. Interferon inhibits dengue virus infection by preventing translation of viral RNA through a PKR-independent mechanism. Virology. 2001;289:297-311 pubmed publisher
    ..We conclude that IFN prevents DV infection by inhibiting translation of the infectious viral RNA through a novel, PKR-independent mechanism...
  70. Chua J, Ng M, Chow V. The non-structural 3 (NS3) protein of dengue virus type 2 interacts with human nuclear receptor binding protein and is associated with alterations in membrane structure. Virus Res. 2004;102:151-63 pubmed publisher
    ..Using yeast two-hybrid and co-immunoprecipitation analyses, we demonstrated that the NS3 protein of dengue virus type 2 interacted specifically with nuclear receptor binding protein (NRBP), a host cellular protein that ..
  71. Falconar A, Martinez F. The NS1 glycoprotein can generate dramatic antibody-enhanced dengue viral replication in normal out-bred mice resulting in lethal multi-organ disease. PLoS ONE. 2011;6:e21024 pubmed publisher
    ..The model provides potential for assessing DENV strain pathogenicity and anti-DENV therapies in normal mice. ..
  72. Hou J, Chen T, Chiang Y, Peng J, Yang T, Cheng C, et al. PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication. Viruses. 2017;9: pubmed publisher
    ..It was ecologically important for virus amplification in mosquitoes and transmission to humans. ..
  73. Monteiro J, Oliveira M, Dias R, Nacif Marçal L, Feio R, Ferreira S, et al. The antimicrobial peptide HS-1 inhibits dengue virus infection. Virology. 2018;514:79-87 pubmed publisher
    ..In vivo tests display 80% protection against the dengue-2 virus due to the presence of HS-1, which reveals its potential as an antiviral against the DENV. ..
  74. Staropoli I, Frenkiel M, M gret F, Deubel V. Affinity-purified dengue-2 virus envelope glycoprotein induces neutralizing antibodies and protective immunity in mice. Vaccine. 1997;15:1946-54 pubmed
  75. Valle R, Falgout B. Mutagenesis of the NS3 protease of dengue virus type 2. J Virol. 1998;72:624-32 pubmed
    ..In all, 46 single-amino-acid substitutions were created in a cloned NS2B-NS3 cDNA fragment of dengue virus type 2, and the effect of each mutation on the extent of self-cleavage of the NS2B-NS3 precursor at the NS2B-NS3 ..
  76. Lindenbach B, Rice C. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J Virol. 1999;73:4611-21 pubmed
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    Recombinant forms of the dengue 2 virus NS3 protease linked to a 40-residue co-factor, corresponding to part of NS2B, have been expressed in Escherichia coli and shown to be active against para-nitroanilide substrates comprising the P6-P1 ..
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    ..p.) virus inoculations, and in both cases, pcTPANS1-immunized mice were protected. Overall, these results provide further support for the use of such a plasmid in a possible approach for the development of a vaccine against DENV...
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    Polyprotein processing of dengue virus type 2, a positive strand RNA virus, is carried out by the host signal peptidase and a novel two-component viral proteinase of the serine proteinase family, NS2B/NS3(Pro), in the endoplasmic ..
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    b>Dengue virus type 2 (DEN2), a member of the Flaviviridae family, is a re-emerging human pathogen of global significance...
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    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...