Dengue virus 1

Summary

Alias: Dengue virus type 1, dengue type 1 D1 virus, dengue virus type 1 DEN1, dengue virus type I, dengue virus-1 DEN-1, type 1 dengue virus DEN-1, Type 1 dengue virus

Top Publications

  1. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002;40:376-81 pubmed
    ..With purified dengue virus type 1 NS1 as a protein standard, the sensitivity of our capture ELISA was less than 1 ng/ml...
  2. Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol. 2005;3:13-22 pubmed publisher
    ..This review focuses mainly on structural studies of dengue virus...
  3. Despres P, Frenkiel M, Deubel V. Differences between cell membrane fusion activities of two dengue type-1 isolates reflect modifications of viral structure. Virology. 1993;196:209-19 pubmed
    ..Changes at positions 96, 297, and 379 map within two overlapping antigenic domains of protein E. These limited amino acid differences in the E protein could affect the biological properties and the antigenicity of the DEN virion. ..
  4. Chen Y, Maguire T, Hileman R, Fromm J, Esko J, Linhardt R, et al. Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nat Med. 1997;3:866-71 pubmed
    ..Similar motifs were also identified in the envelope proteins of other flaviviridae. Developing pharmaceuticals that inhibit target cell binding may be an effective strategy for treating flavivirus infections. ..
  5. Thepparit C, Smith D. Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor. J Virol. 2004;78:12647-56 pubmed
    ..No inhibition of virus entry was seen with dengue virus serotypes 2, 3, or 4, demonstrating that the 37/67-kDa high-affinity laminin receptor is a serotype-specific receptor for dengue virus entry into liver cells. ..
  6. Cui T, Sugrue R, Xu Q, Lee A, Chan Y, Fu J. Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein. Virology. 1998;246:409-17 pubmed
    ..These data suggest that the NTPase activity of the NS3 protein may be regulated by the viral NS5 protein during virus replication. ..
  7. Duarte dos Santos C, Frenkiel M, Courageot M, Rocha C, Vazeille Falcoz M, Wien M, et al. Determinants in the envelope E protein and viral RNA helicase NS3 that influence the induction of apoptosis in response to infection with dengue type 1 virus. Virology. 2000;274:292-308 pubmed
    ..Our data suggest that identified amino acid substitutions in the envelope E protein and viral RNA helicase NS3 may influence DEN-1 virus pathogenicity by altering viral growth. ..
  8. Tassaneetrithep B, Burgess T, Granelli Piperno A, Trumpfheller C, Finke J, Sun W, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med. 2003;197:823-9 pubmed
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection. ..
  9. Zhang W, Chipman P, Corver J, Johnson P, Zhang Y, Mukhopadhyay S, et al. Visualization of membrane protein domains by cryo-electron microscopy of dengue virus. Nat Struct Biol. 2003;10:907-12 pubmed
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus. ..

More Information

Publications110 found, 100 shown here

  1. Hung S, Lee P, Chen H, Chen L, Kao C, King C. Analysis of the steps involved in Dengue virus entry into host cells. Virology. 1999;257:156-67 pubmed
    ..This suggests that cell surface heparan sulfate functions in both viral attachment and penetration of DEN-2 virus. In conclusion, our results further elucidated some aspects of the dengue virus entry process. ..
  2. Roehrig J, Bolin R, Kelly R. Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica. Virology. 1998;246:317-28 pubmed publisher
    ..Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes...
  3. Cardosa M, Wang S, Sum M, Tio P. Antibodies against prM protein distinguish between previous infection with dengue and Japanese encephalitis viruses. BMC Microbiol. 2002;2:9 pubmed
    ..001. The use of flavivirus premembrane protein in seroepidemiological studies will be useful in determining what flaviviruses have circulated in a community...
  4. Purdy D, Chang G. Secretion of noninfectious dengue virus-like particles and identification of amino acids in the stem region involved in intracellular retention of envelope protein. Virology. 2005;333:239-50 pubmed publisher
  5. Umareddy I, Chao A, Sampath A, Gu F, Vasudevan S. Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA. J Gen Virol. 2006;87:2605-14 pubmed publisher
    ..These results suggest that NS4B modulates dengue virus replication via its interaction with NS3...
  6. Rico Hesse R. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology. 1990;174:479-93 pubmed
    ..This is the first genetic evidence of the existence of a sylvatic cycle of dengue virus, which is clearly distinct from outbreak viruses...
  7. Calvert A, Boroughs K, Laven J, Stovall J, Luy B, Kosoy O, et al. Incorporation of IgG Depletion in a Neutralization Assay Facilitates Differential Diagnosis of Zika and Dengue in Secondary Flavivirus Infection Cases. J Clin Microbiol. 2018;56: pubmed publisher
    ..Specimens collected between 1 day and 3 months postexposure were tested for ZIKV and dengue virus type 1 (DENV1) and type 2 (DENV2) by the plaque reduction neutralization test (PRNT) before and after IgG ..
  8. Goncalvez A, Engle R, St Claire M, Purcell R, LAI C. Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proc Natl Acad Sci U S A. 2007;104:9422-7 pubmed publisher
    ..Importantly, a 9-aa deletion at the N terminus of the CH(2) domain in the Fc region abrogated the enhancing activity...
  9. Wan S, Yang Y, Chu Y, Lin C, Chang C, Yeh T, et al. Anti-dengue virus nonstructural protein 1 antibodies contribute to platelet phagocytosis by macrophages. Thromb Haemost. 2016;115:646-56 pubmed publisher
    ..Our study provides evidence for a novel role for anti-DENV NS1 Abs in the pathogenesis of thrombocytopenia in dengue disease by enhancing platelet phagocytosis by macrophages. ..
  10. Poddar A, Ramasamy V, Shukla R, Rajpoot R, Arora U, Jain S, et al. Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies. BMC Biotechnol. 2016;16:50 pubmed publisher
    ..Such efforts could make it possible to envisage the development of a tetravalent dengue vaccine based on VLPs of P. pastoris-expressed E glycoproteins of the four DENV serotypes. ..
  11. Vinayagamoorthy T, Mulatz K, Drebot M, Hodkinson R. Molecular typing of West Nile Virus, Dengue, and St. Louis encephalitis using multiplex sequencing. J Mol Diagn. 2005;7:152-9 pubmed
  12. Silveira G, Strottmann D, DE Borba L, Mansur D, Zanchin N, Bordignon J, et al. Single point mutations in the helicase domain of the NS3 protein enhance dengue virus replicative capacity in human monocyte-derived dendritic cells and circumvent the type I interferon response. Clin Exp Immunol. 2016;183:114-28 pubmed publisher
    ..study, we evaluated the cellular response of human monocyte-derived DCs (mdDCs) infected with recombinant dengue virus type 1 (DV1) strains carrying a single point mutation in the NS3hel protein (L435S or L480S)...
  13. Shah M, Wadood A, RAHMAN Z, Husnain T. Interaction and inhibition of dengue envelope glycoprotein with mammalian receptor DC-sign, an in-silico approach. PLoS ONE. 2013;8:e59211 pubmed publisher
  14. Tan K, Zulkifle N, Abd Jamil J, Sulaiman S, Yaacob C, Azizan N, et al. Disruption of predicted dengue virus type 3 major outbreak cycle coincided with switching of the dominant circulating virus genotype. Infect Genet Evol. 2017;54:271-275 pubmed publisher
    ..In Malaysia, since the 1980s, the major outbreak cycles have involved dengue virus type 3 (DENV3), dengue virus type 1 (DENV1) and dengue virus type 2 (DENV2), occurring in that order (DENV3/DENV1/DENV2)...
  15. Guirakhoo F, Zhang Z, Myers G, Johnson B, Pugachev K, Nichols R, et al. A single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys. J Virol. 2004;78:9998-10008 pubmed publisher
    ..These changes may be responsible for virus attenuation through a change in the pH threshold for virus envelope fusion with the host cell membrane...
  16. Adikari T, Gomes L, Wickramasinghe N, Salimi M, Wijesiriwardana N, Kamaladasa A, et al. Dengue NS1 antigen contributes to disease severity by inducing interleukin (IL)-10 by monocytes. Clin Exp Immunol. 2016;184:90-100 pubmed publisher
    ..Therefore, our data show that dengue NS1 appears to contribute to pathogenesis of dengue infection by inducing IL-10 production by monocytes. ..
  17. Ouyang X, Jiang X, Gu D, Zhang Y, Kong S, Jiang C, et al. Dysregulated Serum MiRNA Profile and Promising Biomarkers in Dengue-infected Patients. Int J Med Sci. 2016;13:195-205 pubmed publisher
    ..Expression levels of broad-spectrum miRNAs in serum samples from three patients with dengue virus type 1 (DENV-1) and three healthy volunteers were separately analyzed using miRNA PCR arrays...
  18. Chang S, Yang C, Hsu T, Su C, Lin C, Shu P. Laboratory-Based Surveillance and Molecular Characterization of Dengue Viruses in Taiwan, 2014. Am J Trop Med Hyg. 2016;94:804-11 pubmed publisher
    ..Phylogenetic analyses showed that genotype I of dengue virus type 1 (DENV-1) and the cosmopolitan genotype of DENV-2 were the predominant DENV strains circulating in southeast ..
  19. de Bruycker Nogueira F, Mir D, dos Santos F, Bello G. Evolutionary history and spatiotemporal dynamics of DENV-1 genotype V in the Americas. Infect Genet Evol. 2016;45:454-460 pubmed publisher
    The genotype V has been the most prevalent dengue virus type 1 (DENV-1) clade circulating in the Americas over the last 40years...
  20. Hsieh S, Wu Y, Zou G, Nerurkar V, Shi P, Wang W. Highly conserved residues in the helical domain of dengue virus type 1 precursor membrane protein are involved in assembly, precursor membrane (prM) protein cleavage, and entry. J Biol Chem. 2014;289:33149-60 pubmed publisher
    ..The highly conserved nature of these residues suggests potential targets of antiviral strategy. ..
  21. Franco L, Pagan I, Serre Del Cor N, Schunk M, Neumayr A, Molero F, et al. Molecular epidemiology suggests Venezuela as the origin of the dengue outbreak in Madeira, Portugal in 2012-2013. Clin Microbiol Infect. 2015;21:713.e5-8 pubmed publisher
    ..Published data showed that dengue virus type 1 introduced from South America was the incriminated virus...
  22. Tan B, Fu J, Sugrue R, Yap E, Chan Y, Tan Y. Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity. Virology. 1996;216:317-25 pubmed publisher
    ..The polymerase product was shown to be a negative-stranded RNA molecule, of template size, which forms a double-stranded complex with the template RNA...
  23. Silva B, Sousa L, Gomes Ruiz A, Leite F, Teixeira M, da Fonseca F, et al. The dengue virus nonstructural protein 1 (NS1) increases NF-?B transcriptional activity in HepG2 cells. Arch Virol. 2011;156:1275-9 pubmed publisher
    ..Luciferase assays showed an increase in NF-?B transcriptional activities in NS1-expressing cells when compared to parental cells. NS1 may enhance NF-?B function in host cells and contribute to the pathogenesis of dengue...
  24. Ishak H, Takegami T, Kamimura K, Funada H. Comparative sequences of two type 1 dengue virus strains possessing different growth characteristics in vitro. Microbiol Immunol. 2001;45:327-31 pubmed
    ..These substitutions might be correlated to their different growth characteristics in vitro. ..
  25. Lin Y, Wen K, Guo Y, Qiu L, Pan Y, Yu L, et al. [Mapping of the B Cell Neutralizing Epitopes on ED III of Envelope Protein from Dengue Virus]. Bing Du Xue Bao. 2015;31:665-73 pubmed
    ..These novel type- and group-specific B-cell epitopes of DENV EDIII may aid help us elucidate the dengue pathogenesis and accelerate vaccine design. ..
  26. Mason P, Z gel M, Semproni A, Fournier M, Mason T. The antigenic structure of dengue type 1 virus envelope and NS1 proteins expressed in Escherichia coli. J Gen Virol. 1990;71 ( Pt 9):2107-14 pubmed publisher
    ..The antibodies elicited by the E fusion protein reacted with a portion of the protein containing domain II, whereas antibodies elicited by the NS1 fusion protein did not react with the antigenic determinants defined by our MAbs...
  27. Chen C, Wei Q. Complete Genome Sequencing of Dengue Virus Type I from Zhuhai City, China. Genome Announc. 2016;4: pubmed publisher
    ..In this study, we reported complete genomic sequences of DENV-1 isolated from Zhuhai patients, providing basic information for future epidemic dengue disease detection. ..
  28. Cunha M, Guimarães V, Souza M, de Paula Cardoso D, de Almeida T, de Oliveira T, et al. Phylodynamics of DENV-1 reveals the spatiotemporal co-circulation of two distinct lineages in 2013 and multiple introductions of dengue virus in Goiás, Brazil. Infect Genet Evol. 2016;43:130-4 pubmed publisher
    b>Dengue virus type 1 (DENV-1) was the first serotype introduced in Brazil, during in the 1980s. Since then, this virus has spread in the Brazilian territory, causing several outbreaks...
  29. Mateu G, Marchevsky R, Liprandi F, Bonaldo M, Coutinho E, Dieudonne M, et al. Construction and biological properties of yellow fever 17D/dengue type 1 recombinant virus. Trans R Soc Trop Med Hyg. 2007;101:289-98 pubmed
    ..This virus is a potential candidate to be included in a tetravalent DEN vaccine formulation. The availability of the cloned cDNA allows further structure/function studies on the viral envelope. ..
  30. Chandramouli S, Joseph J, Daudenarde S, Gatchalian J, Cornillez Ty C, Kuhn P. Serotype-specific structural differences in the protease-cofactor complexes of the dengue virus family. J Virol. 2010;84:3059-67 pubmed publisher
    ..We also report the identification of a 10-residue stretch within NS3pro that separates the substrate-binding function from the catalytic turnover rate of the enzyme. Implications for broad-spectrum drug discovery are discussed...
  31. Huang H, Tseng H, Lee C, Chuang H, Lin S. Clinical significance of skin rash in dengue fever: A focus on discomfort, complications, and disease outcome. Asian Pac J Trop Med. 2016;9:713-8 pubmed publisher
    ..All cases were exclusively caused by type 1 dengue virus. Patients were classified into two groups, based on the presence or absence of skin rash, and their rash ..
  32. Tsai W, Cheng J, Shu C, Lai K, Chan H, Wu C, et al. Asunaprevir Evokes Hepatocytes Innate Immunity to Restrict the Replication of Hepatitis C and Dengue Virus. Front Microbiol. 2017;8:668 pubmed publisher
    ..Our findings reveal an unexpected activity of asunaprevir, the activation of MAVS dependent innate immunity to restrict HCV and DENV infection. ..
  33. Brandt W, Chiewslip D, Harris D, Russell P. Partial purification and characterization of a dengue virus soluble complement-fixing antigen. J Immunol. 1970;105:1565-8 pubmed
  34. Libraty D, Young P, Pickering D, Endy T, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis. 2002;186:1165-8 pubmed publisher
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  35. Rey F. Dengue virus envelope glycoprotein structure: new insight into its interactions during viral entry. Proc Natl Acad Sci U S A. 2003;100:6899-901 pubmed publisher
  36. MacKenzie J, Jones M, Young P. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 1996;220:232-40 pubmed publisher
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  37. Clum S, Ebner K, Padmanabhan R. Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(Pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B. J Biol Chem. 1997;272:30715-23 pubmed
    ..Deletion of hydrophobic regions of NS2B, leaving only the conserved hydrophilic domain of 40 amino acids, resulted in highly efficient processing of the 2B-3 site in vitro in the absence of microsomal membranes...
  38. Jones C, Ma L, Burgner J, Groesch T, Post C, Kuhn R. Flavivirus capsid is a dimeric alpha-helical protein. J Virol. 2003;77:7143-9 pubmed
    ..In contrast, the N terminus was found to be unstructured and could be removed without disrupting the structural integrity of the protein...
  39. Mercado Curiel R, Esquinca Avil s H, Tovar R, D az Badillo A, Camacho Nuez M. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006;6:85 pubmed publisher
    ..aegypti midgut. Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells...
  40. Lin J, Lin S, Chen W, Yen Y, Lai C, Tao M, et al. Dengue viral protease interaction with NF-?B inhibitor ?/? results in endothelial cell apoptosis and hemorrhage development. J Immunol. 2014;193:1258-67 pubmed publisher
    ..In this study, we show that DENV protease participates in the pathogenesis of dengue hemorrhage and discover I?B? and I?B? to be the new cellular targets that are cleaved by DENV protease. ..
  41. Toro J, Salgado D, Vega R, Rodríguez J, Rodríguez L, Angel J, et al. Total and Envelope Protein-Specific Antibody-Secreting Cell Response in Pediatric Dengue Is Highly Modulated by Age and Subsequent Infections. PLoS ONE. 2016;11:e0161795 pubmed publisher
    ..These results are important in the search for correlates of protection and for determining the ideal age for vaccinating against dengue. ..
  42. Li J, Lim S, Beer D, Patel V, Wen D, Tumanut C, et al. Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries. J Biol Chem. 2005;280:28766-74 pubmed publisher
    ..The specificities observed for dengue NS2B/NS3 have features in common with the physiological cleavage sites in the dengue polyprotein; however, all sites reveal previously unrecognized suboptimal sequences...
  43. Mu oz Jord n J, Laurent Rolle M, Ashour J, Mart nez Sobrido L, Ashok M, Lipkin W, et al. Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses. J Virol. 2005;79:8004-13 pubmed publisher
  44. Yocupicio Monroy M, Padmanabhan R, Medina F, Del Angel R. Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells. Virology. 2007;357:29-40 pubmed publisher
    ..Furthermore, the presence of La protein in an in vitro replication system inhibited RNA synthesis in a dose-dependent manner, suggesting that La protein plays an important role in dengue virus replicative cycle...
  45. Jiang L, Yao H, Duan X, Lu X, Liu Y. Polypyrimidine tract-binding protein influences negative strand RNA synthesis of dengue virus. Biochem Biophys Res Commun. 2009;385:187-92 pubmed publisher
    ..This is the first study that enlightens the interaction of DENV NS4A protein with PTB, in addition to demonstrating the novel role of PTB in relation to mosquito-borne flavivirus life-cycle...
  46. Rawlinson S, Pryor M, Wright P, Jans D. Dengue virus RNA polymerase NS5: a potential therapeutic target?. Curr Drug Targets. 2006;7:1623-38 pubmed
    ..This review examines the unique properties of NS5, including its functions, interactions, subcellular localisation and regulation, and looks at ways in which some of these may be exploited in our quest for effective drugs...
  47. Arts R, den Hartog I, Zijlema S, Thijssen V, van der Beelen S, Merkx M. Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone. Anal Chem. 2016;88:4525-32 pubmed publisher
    ..of epitope sequences, as demonstrated here for antibodies against HIV1-p17, hemagglutinin (HA), and dengue virus type I. The combination of sensitive ratiometric bioluminescent detection and the intrinsic modularity of the ..
  48. Johnson A, Guirakhoo F, Roehrig J. The envelope glycoproteins of dengue 1 and dengue 2 viruses grown in mosquito cells differ in their utilization of potential glycosylation sites. Virology. 1994;203:241-9 pubmed publisher
    ..These DEN virus serotypes are thus heterogeneous in their use of glycosylation sites. We also determined by a lectin-binding assay that the attached carbohydrates for both viruses were likely to be of the high-mannose type...
  49. Shiu S, Ip K, Gould E, Chan K. Comparative analysis of the NS 1 gene sequences of dengue-1 viruses prototype Hawaii strain and Thai isolate TH-Sman, and determination of the intratypic variation of NS 1 protein among dengue-1 viruses. Arch Virol. 1993;131:447-54 pubmed
    ..1% to 3.1% difference in the NS 1 protein among dengue-1 strains, which is comparable to that reported for dengue-1 envelope protein (0.2% to 3.6% difference) but less than that of dengue-2 NS 1 protein (0.6% to 7.4% difference). ..
  50. Sugrue R, Fu J, Howe J, Chan Y. Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. J Gen Virol. 1997;78 ( Pt 8):1861-6 pubmed publisher
    ..This is the first report that expression of the structural genes of a flavivirus in yeast is able to generate particulate structures that resemble virions...
  51. Puri B, Nelson W, Henchal E, Hoke C, Eckels K, Dubois D, et al. Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells. J Gen Virol. 1997;78 ( Pt 9):2287-91 pubmed publisher
    ..Important nucleotide base changes responsible for attenuation accumulated between 45AZ5 PDK-O and 27...
  52. Tan B, Fu J, Sugrue R. Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris. Methods Mol Biol. 2007;379:163-76 pubmed
    The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris, using a secretory vector...
  53. Falconar A, Young P. Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1. J Gen Virol. 1991;72 ( Pt 4):961-5 pubmed publisher
  54. Tan C, Yap E, Singh M, Deubel V, Chan Y. Passive protection studies in mice with monoclonal antibodies directed against the non-structural protein NS3 of dengue 1 virus. J Gen Virol. 1990;71 ( Pt 3):745-9 pubmed publisher
    ..Monoclonal antibodies to NS3 of dengue 1 virus are able to increase the survival time of mice challenged with a lethal dose of dengue 1 virus, although the mechanism remains to be defined...
  55. Winkler G, Randolph V, Cleaves G, Ryan T, Stollar V. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology. 1988;162:187-96 pubmed
    ..It is formed between 20 and 40 min after NS1 is synthesized and before the protein passes the Golgi apparatus. Both soluble and pelletable extracellular NS1 are also found as the high-molecular-weight form...
  56. Flamand M, Megret F, Mathieu M, Lepault J, Rey F, Deubel V. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol. 1999;73:6104-10 pubmed
    Nonstructural glycoprotein NS1, specified by dengue virus type 1 (Den-1), is secreted from infected green monkey kidney (Vero) cells in a major soluble form characterized by biochemical and biophysical means as a unique hexameric species...
  57. Raviprakash K, Porter K, Kochel T, Ewing D, Simmons M, Phillips I, et al. Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques. J Gen Virol. 2000;81:1659-67 pubmed publisher
    A candidate DNA vaccine expressing dengue virus type 1 pre-membrane and envelope proteins was used to immunize rhesus macaques. Monkeys were immunized intramuscularly (i.m.) or intradermally (i.d...
  58. Puttikhunt C, Kasinrerk W, Srisa ad S, Duangchinda T, Silakate W, Moonsom S, et al. Production of anti-dengue NS1 monoclonal antibodies by DNA immunization. J Virol Methods. 2003;109:55-61 pubmed
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  59. Clyde K, Harris E. RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication. J Virol. 2006;80:2170-82 pubmed publisher
    ..While numerous regulatory elements have been identified in the untranslated regions of RNA viral genomes, we show that the cHP is a coding-region RNA element that directs start codon selection and is required for viral replication...
  60. Shiryaev S, Ratnikov B, Aleshin A, Kozlov I, Nelson N, Lebl M, et al. Switching the substrate specificity of the two-component NS2B-NS3 flavivirus proteinase by structure-based mutagenesis. J Virol. 2007;81:4501-9 pubmed publisher
  61. Pryor M, Rawlinson S, Butcher R, Barton C, Waterhouse T, Vasudevan S, et al. Nuclear localization of dengue virus nonstructural protein 5 through its importin alpha/beta-recognized nuclear localization sequences is integral to viral infection. Traffic. 2007;8:795-807 pubmed publisher
    ..The results overall indicate that NS5 nuclear localization through the aNLS is integral to viral infection, with significant implications for other flaviviruses of medical importance, such as yellow fever and West Nile viruses...
  62. Lisova O, Hardy F, Petit V, Bedouelle H. Mapping to completeness and transplantation of a group-specific, discontinuous, neutralizing epitope in the envelope protein of dengue virus. J Gen Virol. 2007;88:2387-97 pubmed publisher
    ..The epitope straddles residues of ED3 that are involved in virulence, e.g. Asn/Asp390. These results define the epitope of mAb4E11 as an antigenic signature of the DENV group and suggest mechanisms for its neutralization potency...
  63. Khunchai S, Junking M, Suttitheptumrong A, Yasamut U, Sawasdee N, Netsawang J, et al. Interaction of dengue virus nonstructural protein 5 with Daxx modulates RANTES production. Biochem Biophys Res Commun. 2012;423:398-403 pubmed publisher
    ..This work demonstrates the interaction between DENV NS5 and Daxx and the role of the interaction on the modulation of RANTES production...
  64. Zhang L, Mohan P, Padmanabhan R. Processing and localization of Dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5. J Virol. 1992;66:7549-54 pubmed
    ..Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5...
  65. Bazan J, Fletterick R. Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology. 1989;171:637-9 pubmed
    ..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
  66. Livingston P, Kurane I, Dai L, Okamoto Y, Lai C, Men R, et al. Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities. J Immunol. 1995;154:1287-95 pubmed
    ..These results indicate that CD8+ CTL clones that use different TCR and demonstrate two distinct serotype specificities recognize the same 9-mer peptide in the context of HLA-B35...
  67. Lindenbach B, Rice C. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J Virol. 1999;73:4611-21 pubmed
    ..These findings suggest a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication...
  68. Wang E, Ni H, Xu R, Barrett A, Watowich S, Gubler D, et al. Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. J Virol. 2000;74:3227-34 pubmed
    ..Analysis of envelope protein amino acid changes predicted to have accompanied endemic/epidemic emergence suggested a role for domain III in adaptation to new mosquito and/or human hosts...
  69. Rosen L. Experimental infection of New World monkeys with dengue and yellow fever viruses. Am J Trop Med Hyg. 1958;7:406-10 pubmed
  70. Catteau A, Kalinina O, Wagner M, Deubel V, Courageot M, Despr s P. Dengue virus M protein contains a proapoptotic sequence referred to as ApoptoM. J Gen Virol. 2003;84:2781-93 pubmed publisher
    ..Defining the ApoptoM-activated signalling pathways leading to apoptosis will provide the basis for studying how the M protein might play a key role in the fate of the flavivirus-infected cells...
  71. Roehrig J. Antigenic structure of flavivirus proteins. Adv Virus Res. 2003;59:141-75 pubmed
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  72. Lin C, Chiu S, Hsiao Y, Wan S, Lei H, Shiau A, et al. Expression of cytokine, chemokine, and adhesion molecules during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1. J Immunol. 2005;174:395-403 pubmed
    ..These results suggest the involvement of anti-DV NS1 Abs in the vasculopathy of DV infection...
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    ..At least 3 of the 4 dengue serotypes interact with the 37 kDa/67 kDa laminin receptor, LAMR1, which may be a common player in dengue virus-cell surface interaction...
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    ..Moreover, the conserved motif, 184RKRK, of NS3 played a crucial role in binding to RNA substrate and modulating the NTPase/RNA helicase and RTPase activities of NS3...
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    ..These observations provide very useful messages with physiological significance in investigation of the pathogenesis, the defense mechanisms of human hosts and the therapeutic considerations in DV infection...
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    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
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    ..7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories...
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    ..These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays...
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    The complete nucleotide sequence and the deduced amino acid sequence of the genome of dengue virus type 1 (Singapore strain S275/90) were determined from cDNA clones...
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    ..A dose/response effect was observed, with increasing amounts of recombinant antigen leading to increased survival. These results demonstrate the utility of baculovirus for producing immunogens against DEN-1 virus...
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    ..The system described herein offers an opportunity to explore DEN replication in vivo and to test various antiviral protocols in infected hosts...
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    ..These results extend our understanding of the structure-function relationships in the E glycoprotein of DEN virus and provide the first direct evidence that domain III encodes the primary flavivirus receptor-binding motif...
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    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
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    ..We believe this is the first report of a pathogen interacting more efficiently with DC-SIGNR than with DC-SIGN. Our results should lead to the discovery of new mechanisms by which these well-studied lectins discriminate among ligands...
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    ..Complement activation mediated by NS1 leads to local and systemic generation of anaphylatoxins and SC5b-9, which may contribute to the pathogenesis of the vascular leakage that occurs in patients with DHF/DSS...
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    ..Based on these findings, we hypothesize that anti-DV NS1 is involved in the pathogenesis of DF and DHF/DSS, and this may provide important information in dengue vaccine development...
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    ..These results indicate an association of a minor subpopulation of NS1 with lipid rafts during dengue virus infection and suggest that modification of NS1, possibly lipidation, is required for raft association...
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    ..No difference was observed, however, in Indonesian patients with secondary infection who had dengue fever or DHF...
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