Human papillomavirus type 16


Alias: HPV16, human papillomavirus type 16 HPV 16, human papillomavirus type 16 HPV16

Top Publications

  1. Yasugi T, Vidal M, Sakai H, Howley P, Benson J. Two classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization, E2 interaction, and interaction with cellular proteins. J Virol. 1997;71:5942-51 pubmed
    Random mutagenesis of human papillomavirus type 16 (HPV16) E1 was used to generate E1 missense mutants defective for interaction with either hUBC9 or 16E1-BP, two cDNAs encoding proteins that have been identified by their ability to ..
  2. Murvai M, Borbely A, Konya J, Gergely L, Veress G. Effect of human papillomavirus type 16 E6 and E7 oncogenes on the activity of the transforming growth factor-beta2 (TGF-beta2) promoter. Arch Virol. 2004;149:2379-92 pubmed
    The effect of the human papillomavirus type 16 (HPV 16) E6 and E7 proteins was studied on the transcriptional activity of the human transforming growth factor beta2 (TGF-beta) promoter in different cell lines...
  3. Liu X, Yuan H, Fu B, Disbrow G, Apolinario T, Tomaic V, et al. The E6AP ubiquitin ligase is required for transactivation of the hTERT promoter by the human papillomavirus E6 oncoprotein. J Biol Chem. 2005;280:10807-16 pubmed
  4. Soeda E, Ferran M, Baker C, McBride A. Repression of HPV16 early region transcription by the E2 protein. Virology. 2006;351:29-41 pubmed
    b>HPV16 DNA is often integrated in cancers, disrupting the E1 or E2 genes. E2 can repress the E6/E7 promoter, but other models have been proposed to explain why integration promotes malignant progression...
  5. Jing M, Bohl J, Brimer N, Kinter M, Vande Pol S. Degradation of tyrosine phosphatase PTPN3 (PTPH1) by association with oncogenic human papillomavirus E6 proteins. J Virol. 2007;81:2231-9 pubmed
    ..This report demonstrates the potential of E6 to regulate phosphotyrosine metabolism through the targeted degradation of a tyrosine phosphatase. ..
  6. Severino A, Abbruzzese C, Manente L, Valderas A, Mattarocci S, Federico A, et al. Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human immortalized keratinocytes. J Cell Physiol. 2007;212:118-25 pubmed
  7. Florin L, Becker K, Sapp C, Lambert C, Sirma H, Muller M, et al. Nuclear translocation of papillomavirus minor capsid protein L2 requires Hsc70. J Virol. 2004;78:5546-53 pubmed
    ..These data indicate that Hsc70 transiently associates with viral capsids during the integration of L2, possibly via the L2 C terminus. Completion of virus assembly results in displacement of Hsc70 from virions. ..
  8. Auvinen E, Alonso A, Auvinen P. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol. 2004;149:1745-59 pubmed
    b>Human papillomavirus type 16 E5 protein contributes to cellular transformation by increasing the mitogenic stimulus from growth factor receptors to the nucleus...
  9. Zhang B, Chen W, Roman A. The E7 proteins of low- and high-risk human papillomaviruses share the ability to target the pRB family member p130 for degradation. Proc Natl Acad Sci U S A. 2006;103:437-42 pubmed
    ..The added ability of HPV-16 E7 to regulate pRB and p107 may be related to oncogenic activity. ..

More Information

Publications101 found, 100 shown here

  1. Tang S, Tao M, McCoy J, Zheng Z. The E7 oncoprotein is translated from spliced E6*I transcripts in high-risk human papillomavirus type 16- or type 18-positive cervical cancer cell lines via translation reinitiation. J Virol. 2006;80:4249-63 pubmed
    ..Two other siRNAs targeting the exon 2 regions of HPV16 and -18, which encode the E7 oncoprotein, reduced the E6*I mRNAs to a remarkable extent and preferentially ..
  2. Cooper B, Brimer N, Vande Pol S. Human papillomavirus E6 regulates the cytoskeleton dynamics of keratinocytes through targeted degradation of p53. J Virol. 2007;81:12675-9 pubmed
    ..b>Human papillomavirus type 16 (HPV-16) E6 augmented the kinetics of cell spreading, while E6 genes from HPV-11 or bovine ..
  3. Pillai M, Hariharan R, Babu J, Lakshmi S, Chiplunkar S, Patkar M, et al. Molecular variants of HPV-16 associated with cervical cancer in Indian population. Int J Cancer. 2009;125:91-103 pubmed publisher
    Human papilloma virus is a causative factor in the etiology of cervical cancer with HPV16 being the most prevalent genotype associated with it...
  4. Rosenberger S, De Castro Arce J, Langbein L, Steenbergen R, Rösl F. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation. Proc Natl Acad Sci U S A. 2010;107:7006-11 pubmed publisher
    ..Here we show that splicing of HPV16 E6/E7 ORF cassette is regulated by the epidermal growth factor (EGF) pathway...
  5. Nicolaides L, Davy C, Raj K, Kranjec C, Banks L, Doorbar J. Stabilization of HPV16 E6 protein by PDZ proteins, and potential implications for genome maintenance. Virology. 2011;414:137-45 pubmed publisher
  6. Boulabiar M, Boubaker S, Favre M, Demeret C. Keratinocyte sensitization to tumour necrosis factor-induced nuclear factor kappa B activation by the E2 regulatory protein of human papillomaviruses. J Gen Virol. 2011;92:2422-7 pubmed publisher
    ..Since NF-?B controls epithelial differentiation, this activity may be involved in the commitment of infected keratinocytes to proliferation arrest and differentiation, both required for the implementation of the productive viral cycle. ..
  7. Gyöngyösi E, Szalmás A, Ferenczi A, Konya J, Gergely L, Veress G. Effects of human papillomavirus (HPV) type 16 oncoproteins on the expression of involucrin in human keratinocytes. Virol J. 2012;9:36 pubmed publisher
    ..Here, we aimed to study the effects of HPV16 E6 and E7 oncogenes on the expression of involucrin (IVL), an established marker of keratinocyte differentiation, ..
  8. Niccoli S, Abraham S, Richard C, Zehbe I. The Asian-American E6 variant protein of human papillomavirus 16 alone is sufficient to promote immortalization, transformation, and migration of primary human foreskin keratinocytes. J Virol. 2012;86:12384-96 pubmed publisher
    ..Our findings are in line with epidemiological data that the AA variant of HPV16 confers an increased risk over the European prototype for cervical cancer, as evidenced by a superior ..
  9. Zhang B, Laribee R, Klemsz M, Roman A. Human papillomavirus type 16 E7 protein increases acetylation of histone H3 in human foreskin keratinocytes. Virology. 2004;329:189-98 pubmed
    ..Acetylation of histones is regulated by histone acetyltransferases and histone deacetylases (HDACs). Human papillomavirus type 16 (HPV16) E7 can inactivate retinoblastoma protein (pRB), which recruits histone deacetylases, and also ..
  10. Bose K, Yoder N, Kumar K, Baleja J. The role of conserved histidines in the structure and stability of human papillomavirus type 16 E2 DNA-binding domain. Biochemistry. 2007;46:1402-11 pubmed
    ..Moreover, identification of residues crucial for E2 stability will help in the design of modified proteins with desired characteristics. ..
  11. Campos S, Ozbun M. Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes. PLoS ONE. 2009;4:e4463 pubmed publisher
    ..A neutralizing B cell epitope has recently been mapped to the N-terminus of HPV16 L2, residues 17-36, and exposure of this region of L2 has been implicated in translocation of incoming virions ..
  12. Wanichwatanadecha P, Sirisrimangkorn S, Kaewprag J, Ponglikitmongkol M. Transactivation activity of human papillomavirus type 16 E6*I on aldo-keto reductase genes enhances chemoresistance in cervical cancer cells. J Gen Virol. 2012;93:1081-92 pubmed publisher
    ..High-risk HPVs, including HPV16, have the unique ability to splice the E6 viral transcript, resulting in the production of a truncated E6 protein ..
  13. Malanchi I, Accardi R, Diehl F, Smet A, Androphy E, Hoheisel J, et al. Human papillomavirus type 16 E6 promotes retinoblastoma protein phosphorylation and cell cycle progression. J Virol. 2004;78:13769-78 pubmed
    We show that E6 proteins from benign human papillomavirus type 1 (HPV1) and oncogenic HPV16 have the ability to alter the regulation of the G(1)/S transition of the cell cycle in primary human fibroblasts...
  14. Egawa N, Nakahara T, Ohno S, Narisawa Saito M, Yugawa T, Fujita M, et al. The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J Virol. 2012;86:3276-83 pubmed publisher
    ..for E1 in viral DNA replication at different stages, an E1-defective mutant of the human papillomavirus 16 (HPV16) genome featuring a translation termination mutation in the E1 gene was used...
  15. Baker C, Phelps W, Lindgren V, Braun M, Gonda M, Howley P. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J Virol. 1987;61:962-71 pubmed
    We cloned and analyzed the integrated human papillomavirus type 16 (HPV-16) genomes that are present in the human cervical carcinoma cell lines SiHa and CaSki...
  16. Gammoh N, Grm H, Massimi P, Banks L. Regulation of human papillomavirus type 16 E7 activity through direct protein interaction with the E2 transcriptional activator. J Virol. 2006;80:1787-97 pubmed
    ..These results demonstrate a direct role for E2 in regulating the function of E7 and suggest an important role for E2 in directing E7 localization during mitosis. ..
  17. Spardy N, Duensing A, Charles D, Haines N, Nakahara T, Lambert P, et al. The human papillomavirus type 16 E7 oncoprotein activates the Fanconi anemia (FA) pathway and causes accelerated chromosomal instability in FA cells. J Virol. 2007;81:13265-70 pubmed
  18. Yoshida T, Sano T, Kanuma T, Owada N, Sakurai S, Fukuda T, et al. Immunochemical analysis of HPV L1 capsid protein and p16 protein in liquid-based cytology samples from uterine cervical lesions. Cancer. 2008;114:83-8 pubmed publisher
    ..The correlation between L1 and p16 expressions suggests that L1(-)/p16(+) cases have the potential for progression, whereas L1(+)/p16(-) and L1(-)/p16(-) cases may be nonprogressive lesions or potentially in remission. ..
  19. Ristriani T, Fournane S, Orfanoudakis G, Trave G, Masson M. A single-codon mutation converts HPV16 E6 oncoprotein into a potential tumor suppressor, which induces p53-dependent senescence of HPV-positive HeLa cervical cancer cells. Oncogene. 2009;28:762-72 pubmed publisher
    High-risk mucosal human papillomaviruses (HPV), mainly HPV16 and HPV18, are implicated in cervical carcinogenesis...
  20. Davy C, McIntosh P, Jackson D, Sorathia R, Miell M, Wang Q, et al. A novel interaction between the human papillomavirus type 16 E2 and E1--E4 proteins leads to stabilization of E2. Virology. 2009;394:266-75 pubmed publisher
    The E4 (also called E1--E4) and E2 proteins of human papillomavirus type 16 are thought to be expressed within the same cells of a lesion, and their open reading frames overlap, suggesting that they may have a functional relationship...
  21. You J, Croyle J, Nishimura A, Ozato K, Howley P. Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. Cell. 2004;117:349-60 pubmed
    ..Brd4 also associates with HPV16 E2, indicating that Brd4 binding may be a shared property of all papillomavirus E2 proteins...
  22. Okoye A, Cordano P, Taylor E, Morgan I, Everett R, Campo M. Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2. Virus Res. 2005;108:1-14 pubmed
    ..The consequences of the L2-E2 interaction for the viral life cycle are discussed. ..
  23. Nakahara T, Peh W, Doorbar J, Lee D, Lambert P. Human papillomavirus type 16 E1circumflexE4 contributes to multiple facets of the papillomavirus life cycle. J Virol. 2005;79:13150-65 pubmed
    ..of the viral E1(circumflex)E4 protein in the HPV life cycle, we characterized the properties of HPV type 16 (HPV16) genomes harboring mutations in the E4 gene in NIKS cells, a spontaneously immortalized keratinocyte cell line ..
  24. Garcia Alai M, Alonso L, de Prat Gay G. The N-terminal module of HPV16 E7 is an intrinsically disordered domain that confers conformational and recognition plasticity to the oncoprotein. Biochemistry. 2007;46:10405-12 pubmed
    The HPV16 E7 oncoprotein is an extended dimer, with a stable and cooperative fold, but that displays properties of "natively unfolded" proteins...
  25. Nguyen C, Eichwald C, Nibert M, Munger K. Human papillomavirus type 16 E7 oncoprotein associates with the centrosomal component gamma-tubulin. J Virol. 2007;81:13533-43 pubmed
    ..HPV type 16 (HPV16) E7 induces supernumerary centrosomes through a mechanism that is at least in part independent of the inactivation ..
  26. Johansson C, Graham S, Dornan E, Morgan I. The human papillomavirus 16 E2 protein is stabilised in S phase. Virology. 2009;394:194-9 pubmed publisher
    ..Preliminary studies have identified E2 as a Cdk2 substrate demonstrating this enzyme as a candidate kinase for mediating the in vivo phosphorylation of HPV16 E2.
  27. McKenna D, McDade S, Patel D, McCance D. MicroRNA 203 expression in keratinocytes is dependent on regulation of p53 levels by E6. J Virol. 2010;84:10644-52 pubmed publisher
    ..We investigated how expression of human papillomavirus type 16 (HPV16) oncoproteins E6 and E7 affected miR-203 expression during proliferation and differentiation of ..
  28. Jabbar S, Strati K, Shin M, Pitot H, Lambert P. Human papillomavirus type 16 E6 and E7 oncoproteins act synergistically to cause head and neck cancer in mice. Virology. 2010;407:60-7 pubmed publisher
    ..Comparing the oncogenic properties of wild-type versus mutant E6 genes in this model for HNSCC uncovered a role for some but not other cellular targets of E6 previously shown to contribute to cervical cancer. ..
  29. Chang S, Tsao Y, Lin C, Chen S. NRIP, a novel calmodulin binding protein, activates calcineurin to dephosphorylate human papillomavirus E2 protein. J Virol. 2011;85:6750-63 pubmed publisher
    ..We present evidences for E2 phosphorylation in vivo and show that NRIP acts as a scaffold to recruit E2 and calcium/calmodulin to prevent polyubiquitination and degradation of E2, enhancing E2 stability and E2-driven gene expression. ..
  30. Todorovic B, Massimi P, Hung K, Shaw G, Banks L, Mymryk J. Systematic analysis of the amino acid residues of human papillomavirus type 16 E7 conserved region 3 involved in dimerization and transformation. J Virol. 2011;85:10048-57 pubmed publisher
    ..To systematically analyze the molecular mechanisms by which HPV16 E7 CR3 contributes to carcinogenesis, we created a comprehensive panel of mutations in residues predicted to be ..
  31. Reiser J, Hurst J, Voges M, Krauss P, Münch P, Iftner T, et al. High-risk human papillomaviruses repress constitutive kappa interferon transcription via E6 to prevent pathogen recognition receptor and antiviral-gene expression. J Virol. 2011;85:11372-80 pubmed publisher
    Persistent infections with human papillomavirus type 16 (HPV16), HPV18, or HPV31 are necessary for the development of cervical cancer, implying that HPVs have evolved immunoevasive mechanisms...
  32. Shamanin V, Sekaric P, Androphy E. hAda3 degradation by papillomavirus type 16 E6 correlates with abrogation of the p14ARF-p53 pathway and efficient immortalization of human mammary epithelial cells. J Virol. 2008;82:3912-20 pubmed publisher
    Two activities of human papillomavirus type 16 E6 (HPV16 E6) are proposed to contribute to the efficient immortalization of human epithelial cells: the degradation of p53 protein and the induction of telomerase...
  33. Yan J, Li Q, Lievens S, Tavernier J, You J. Abrogation of the Brd4-positive transcription elongation factor B complex by papillomavirus E2 protein contributes to viral oncogene repression. J Virol. 2010;84:76-87 pubmed publisher
    ..Abrogation of the interaction between P-TEFb and Brd4 thus provides a mechanism for E2-mediated repression of the viral oncogenes from the integrated viral genomes in cancer cells. ..
  34. Dreier K, Scheiden R, Lener B, Ehehalt D, Pircher H, Muller Holzner E, et al. Subcellular localization of the human papillomavirus 16 E7 oncoprotein in CaSki cells and its detection in cervical adenocarcinoma and adenocarcinoma in situ. Virology. 2011;409:54-68 pubmed publisher
    ..Our findings suggest that the HPV-16 E7 oncoprotein could be a useful marker for the detection of cervical adenocarcinoma and their precursors. ..
  35. Zehbe I, Lichtig H, Westerback A, Lambert P, Tommasino M, Sherman L. Rare human papillomavirus 16 E6 variants reveal significant oncogenic potential. Mol Cancer. 2011;10:77 pubmed publisher
    ..The data suggests that differences in E6 variant prevalence in cervical carcinoma may not be related to the carcinogenic potential of the E6 protein. ..
  36. Chang S, Lu P, Guo J, Tsai T, Tsao Y, Chen S. NRIP enhances HPV gene expression via interaction with either GR or E2. Virology. 2012;423:38-48 pubmed publisher
    ..These results indicate that NRIP and GR are viral E2-binding proteins and that NRIP regulates HPV gene expression via GRE and/or E2 binding site in the HPV promoter in a hormone-dependent or independent manner, respectively. ..
  37. Muller M, Jacob Y, Jones L, Weiss A, Brino L, Chantier T, et al. Large scale genotype comparison of human papillomavirus E2-host interaction networks provides new insights for e2 molecular functions. PLoS Pathog. 2012;8:e1002761 pubmed publisher
    ..These include transcription regulation, regulation of apoptosis, RNA processing, ubiquitination and intracellular trafficking. The present work provides an overview of E2 biological functions across multiple HPV genotypes. ..
  38. Gao L, Gu P, Zhao W, Ding W, Zhao X, Guo S, et al. The role of globular heads of the C1q receptor in HPV 16 E2-induced human cervical squamous carcinoma cell apoptosis is associated with p38 MAPK/JNK activation. J Transl Med. 2013;11:118 pubmed publisher
    b>Human papillomavirus type 16 (HPV 16) E2 protein is a multifunctional DNA-binding protein. HPV 16 E2 regulates many biological responses, including DNA replication, gene expression, and apoptosis...
  39. Niebler M, Qian X, Höfler D, Kogosov V, Kaewprag J, Kaufmann A, et al. Post-translational control of IL-1? via the human papillomavirus type 16 E6 oncoprotein: a novel mechanism of innate immune escape mediated by the E3-ubiquitin ligase E6-AP and p53. PLoS Pathog. 2013;9:e1003536 pubmed publisher
    ..Here, we describe a novel mechanism how the high-risk HPV16 E6 oncoprotein abrogates IL-1? processing and secretion in a NALP3 inflammasome-independent manner...
  40. Seedorf K, Krammer G, Durst M, Suhai S, Rowekamp W. Human papillomavirus type 16 DNA sequence. Virology. 1985;145:181-5 pubmed
    The complete nucleotide sequence of HPV16 DNA (7904 bp) cloned from an invasive cervical carcinoma was determined...
  41. Darshan M, Lucchi J, Harding E, Moroianu J. The l2 minor capsid protein of human papillomavirus type 16 interacts with a network of nuclear import receptors. J Virol. 2004;78:12179-88 pubmed
    ..In this study we investigated the interactions between the L2 protein of high-risk human papillomavirus type 16 (HPV16) and nuclear import receptors...
  42. Mouret S, Sauvaigo S, Peinnequin A, Favier A, Beani J, Leccia M. E6* oncoprotein expression of human papillomavirus type-16 determines different ultraviolet sensitivity related to glutathione and glutathione peroxidase antioxidant defence. Exp Dermatol. 2005;14:401-10 pubmed
    ..aim of the present study is to evaluate UV sensitivity and DNA damage formation according to antioxidant status in HPV16-infected keratinocytes...
  43. Ishii Y, Ozaki S, Tanaka K, Kanda T. Human papillomavirus 16 minor capsid protein L2 helps capsomeres assemble independently of intercapsomeric disulfide bonding. Virus Genes. 2005;31:321-8 pubmed
    ..To study a possible role of L2 in capsid assembly, we examined the interaction between HPV16 L2 and capsomeres under the conditions that inhibit the formation of disulfide bonds in vitro and in vivo...
  44. Filippova M, Johnson M, Bautista M, Filippov V, Fodor N, Tungteakkhun S, et al. The large and small isoforms of human papillomavirus type 16 E6 bind to and differentially affect procaspase 8 stability and activity. J Virol. 2007;81:4116-29 pubmed
    b>Human papillomavirus type 16 (HPV-16) has developed numerous ways to modulate host-initiated immune mechanisms...
  45. Asadurian Y, Kurilin H, Lichtig H, Jackman A, Gonen P, Tommasino M, et al. Activities of human papillomavirus 16 E6 natural variants in human keratinocytes. J Med Virol. 2007;79:1751-60 pubmed
    ..Ten HPV16 E6 variants containing amino acid substitutions in the N-terminal region of E6 were evaluated for different ..
  46. Knapp A, McManus P, Bockstall K, Moroianu J. Identification of the nuclear localization and export signals of high risk HPV16 E7 oncoprotein. Virology. 2009;383:60-8 pubmed publisher
    The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins...
  47. Smotkin D, Prokoph H, Wettstein F. Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J Virol. 1989;63:1441-7 pubmed
    ..No equivalent promoter was active in HPV16-containing cancers and cancer-derived cell lines...
  48. Matsumoto Y, Nakagawa S, Yano T, Takizawa S, Nagasaka K, Nakagawa K, et al. Involvement of a cellular ubiquitin-protein ligase E6AP in the ubiquitin-mediated degradation of extensive substrates of high-risk human papillomavirus E6. J Med Virol. 2006;78:501-7 pubmed
    ..These data revealed that E6AP is extensively involved in the ubiquitin-mediated degradation of E6-dependent substrates as a cellular E3 ubiquitin-protein ligase. ..
  49. Donaldson M, Boner W, Morgan I. TopBP1 regulates human papillomavirus type 16 E2 interaction with chromatin. J Virol. 2007;81:4338-42 pubmed
    b>Human papillomavirus type 16 (HPV16) E2 regulates transcription from and replication of the viral genome, in association with viral and cellular factors...
  50. Suprynowicz F, Disbrow G, Krawczyk E, Simic V, Lantzky K, Schlegel R. HPV-16 E5 oncoprotein upregulates lipid raft components caveolin-1 and ganglioside GM1 at the plasma membrane of cervical cells. Oncogene. 2008;27:1071-8 pubmed
    ..Finally, the upregulation of caveolin-1 and ganglioside GM1 at the plasma membrane of E5-expressing cervical cells provides potential new therapeutic targets and diagnostic markers for high-risk HPV infections. ..
  51. Santer F, Moser B, Spoden G, Jansen Durr P, Zwerschke W. Human papillomavirus type 16 E7 oncoprotein inhibits apoptosis mediated by nuclear insulin-like growth factor-binding protein-3 by enhancing its ubiquitin/proteasome-dependent degradation. Carcinogenesis. 2007;28:2511-20 pubmed
    The E7 protein encoded by the oncogenic human papillomavirus type 16 has been shown to bind and inactivate insulin-like growth factor-binding protein-3 (IGFBP-3), the pro-apoptotic product of a tumour suppressor gene; however, the ..
  52. Hebner C, Beglin M, Laimins L. Human papillomavirus E6 proteins mediate resistance to interferon-induced growth arrest through inhibition of p53 acetylation. J Virol. 2007;81:12740-7 pubmed
    ..This study identifies an important physiological role for E6 binding to p300/CBP in blocking growth arrest of human keratinocytes in the presence of interferon and so contributes to the persistence of HPV-infected cells. ..
  53. Allison S, Jiang M, Milner J. Oncogenic viral protein HPV E7 up-regulates the SIRT1 longevity protein in human cervical cancer cells. Aging (Albany NY). 2009;1:316-27 pubmed
    ..This link may open the way for a more in-depth understanding of the process of HPV-induced malignant transformation and also of the inter-relationships between aging and cancer. ..
  54. McCloskey R, Menges C, Friedman A, Patel D, McCance D. Human papillomavirus type 16 E6/E7 upregulation of nucleophosmin is important for proliferation and inhibition of differentiation. J Virol. 2010;84:5131-9 pubmed publisher
    ..The results show for the first time that NPM is required for the proliferation and inhibition of differentiation observed in HPV E6- and E7-expressing primary cells. ..
  55. Park J, Pitot H, Strati K, Spardy N, Duensing S, Grompe M, et al. Deficiencies in the Fanconi anemia DNA damage response pathway increase sensitivity to HPV-associated head and neck cancer. Cancer Res. 2010;70:9959-68 pubmed publisher
    ..Human papillomaviruses (HPVs), particularly HPV16, are associated with ?20% of head and neck squamous cell carcinomas (HNSCCs) in the general population...
  56. Au Yeung C, Tsang T, Yau P, Kwok T. Human papillomavirus type 16 E6 induces cervical cancer cell migration through the p53/microRNA-23b/urokinase-type plasminogen activator pathway. Oncogene. 2011;30:2401-10 pubmed publisher
    ..Therefore, p53 is believed to mediate the HPV-16 E6 downregulation of miR-23b. From the above, miR-23b/uPA are confirmed to be involved in HPV-16 E6-associated cervical cancer development. ..
  57. Mesplede T, Gagnon D, Bergeron Labrecque F, Azar I, Sénéchal H, Coutlee F, et al. p53 degradation activity, expression, and subcellular localization of E6 proteins from 29 human papillomavirus genotypes. J Virol. 2012;86:94-107 pubmed publisher measure the p53 degradation activities of E6 proteins from 29 prevalent HPV types and variants of HPV type 16 (HPV16) and HPV33 by determining the amount of E6 expression vector required to reduce by half the levels of RLuc-p53 (50%..
  58. Bienkowska Haba M, Williams C, Kim S, Garcea R, Sapp M. Cyclophilins facilitate dissociation of the human papillomavirus type 16 capsid protein L1 from the L2/DNA complex following virus entry. J Virol. 2012;86:9875-87 pubmed publisher
    ..At the outset of infection, the interaction of HPV type 16 (HPV16) (pseudo)virions with heparan sulfate proteoglycans triggers a conformational change in L2 that is facilitated by ..
  59. Choyce A, Yong M, Narayan S, Mattarollo S, Liem A, Lambert P, et al. Expression of a single, viral oncoprotein in skin epithelium is sufficient to recruit lymphocytes. PLoS ONE. 2013;8:e57798 pubmed publisher
    ..In a mouse model of premalignant skin epithelium, transgenic mice that express the human papillomavirus type 16 (HPV16) E7 oncoprotein under a keratin 14 promoter (K14E7 mice) display epidermal hyperplasia and have ..
  60. Nelson L, Rose R, Moroianu J. Nuclear import strategies of high risk HPV16 L1 major capsid protein. J Biol Chem. 2002;277:23958-64 pubmed publisher
    ..We investigated the nuclear import of the L1 major capsid protein of high risk HPV16. When digitonin-permeabilized HeLa cells were incubated with HPV16 L1 capsomeres, the L1 protein was imported into ..
  61. Olcese V, Chen Y, Schlegel R, Yuan H. Characterization of HPV16 L1 loop domains in the formation of a type-specific, conformational epitope. BMC Microbiol. 2004;4:29 pubmed publisher
    ..To investigate the molecular determinants of the HPV16 L1 conformational epitope recognized by monoclonal antibody 16A, we utilized a domain-swapping approach to ..
  62. Thomas M, Chiang C. E6 oncoprotein represses p53-dependent gene activation via inhibition of protein acetylation independently of inducing p53 degradation. Mol Cell. 2005;17:251-64 pubmed publisher
  63. Davy C, Ayub M, Jackson D, Das P, McIntosh P, Doorbar J. HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm. Virology. 2006;349:230-44 pubmed publisher
    The human papillomavirus type 16 E1--E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression...
  64. Shai A, Nguyen M, Wagstaff J, Jiang Y, Lambert P. HPV16 E6 confers p53-dependent and p53-independent phenotypes in the epidermis of mice deficient for E6AP. Oncogene. 2007;26:3321-8 pubmed publisher
    ..In mice transgenic for wild-type HPV16 E6, its expression leads to epithelial hyperplasia and an abrogation of normal cellular responses to DNA damage...
  65. Narisawa Saito M, Handa K, Yugawa T, Ohno S, Fujita M, Kiyono T. HPV16 E6-mediated stabilization of ErbB2 in neoplastic transformation of human cervical keratinocytes. Oncogene. 2007;26:2988-96 pubmed publisher
    ..Furthermore, increased ErbB2 expression was also observed in HPV16 positive cervical cancer cell lines and this was diminished by introduction of HPV16E6- or E6AP-shRNA...
  66. Storrs C, Silverstein S. PATJ, a tight junction-associated PDZ protein, is a novel degradation target of high-risk human papillomavirus E6 and the alternatively spliced isoform 18 E6. J Virol. 2007;81:4080-90 pubmed publisher
    ..We also demonstrate that 18 E6-mediated degradation of PATJ is not inhibited in cells where E6AP is silenced by shRNA. This suggests that the E6-E6AP complex is not required for the degradation of this protein target...
  67. Woo M, Hur S, Park S, Kim H. Study of cell-mediated response in mice by HPV16 L1 virus-like particles expressed in Saccharomyces cerevisiae. J Microbiol Biotechnol. 2007;17:1738-41 pubmed
    The first vaccine against human papillomaviruses (HPV) formulated with HPV16 L1 virus-like particles (VLPs) produced in yeast was approved by the FDA in June 2006...
  68. Clawson G, Bui V, Xin P, Wang N, Pan W. Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. J Cell Biochem. 2008;105:81-8 pubmed publisher
    ..Further, expression of HPV E6/E7 proteins is associated with a post-transcriptional up-regulation of HtrA1 (most notably the nuclear form), and HtrA1 is found associated with both HPV E6 and E7 proteins...
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    ..Expression of HPV16 E7 in normal human epithelial cells caused increased levels of vimentin and fibronectin, whereas the epithelial ..
  70. Zheng G, Schweiger M, Martinez Noel G, Zheng L, Smith J, Harper J, et al. Brd4 regulation of papillomavirus protein E2 stability. J Virol. 2009;83:8683-92 pubmed publisher
    ..In this study, we explored the role of Brd4 in the regulation of bovine PV 1 (BPV1) and human PV 16 (HPV16) E2 stability. Expression of the Brd4 CTD dramatically increases E2 levels...
  71. Hu L, Ceresa B. Characterization of the plasma membrane localization and orientation of HPV16 E5 for cell-cell fusion. Virology. 2009;393:135-43 pubmed publisher
    ..Three genes encoded by HPV16 are regarded as oncogenic - E5, E6, and E7. The role of E5 has been controversial...
  72. Yamakawa Kakuta Y, Kawamata H, Doi Y, Fujimori T, Imai Y. Does the expression of HPV16/18 E6/E7 in head and neck squamous cell carcinomas relate to their clinicopathological characteristics?. Int J Oncol. 2009;35:983-8 pubmed
    ..We examined the expression of HPV16/18 E6/E7 in 71 cases of HNSCCs and investigated abnormalities of the p53 gene in 62 of these 71 cases...
  73. Li W, Deng X, Wang C, Zhang X, Zheng G, Zhang J, et al. Down-regulation of HLA class I antigen in human papillomavirus type 16 E7 expressing HaCaT cells: correlate with TAP-1 expression. Int J Gynecol Cancer. 2010;20:227-32 pubmed publisher
    ..001). Our finding demonstrates that HPV-16 E7 down-regulates surface HLA class I antigen, which in part correlates with the decrease of TAP-1. ..
  74. Kanduc D. Potential cross-reactivity between HPV16 L1 protein and sudden death-associated antigens. J Exp Ther Oncol. 2011;9:159-65 pubmed
    ..The present data may help evaluate the potential crossreactivity risks in anti-tumor vaccination protocols based on HPV16 L1 protein.
  75. Thomas M, Pitot H, Liem A, Lambert P. Dominant role of HPV16 E7 in anal carcinogenesis. Virology. 2011;421:114-8 pubmed publisher
    ..These findings indicate that E7 is the more potent oncogene in anal cancer caused by HPVs...
  76. Ren C, Cheng X, Lu B, Yang G. Activation of interleukin-6/signal transducer and activator of transcription 3 by human papillomavirus early proteins 6 induces fibroblast senescence to promote cervical tumourigenesis through autocrine and paracrine pathways in tumour microenvironme. Eur J Cancer. 2013;49:3889-99 pubmed publisher
    ..Thus, we have uncovered a mechanism that fibroblast senescence promotes cervical cancer development through high-risk HPV E6-activated IL-6/STAT3 signalling in tumour microenvironment...
  77. Vaeteewoottacharn K, Chamutpong S, Ponglikitmongkol M, Angeletti P. Differential localization of HPV16 E6 splice products with E6-associated protein. Virol J. 2005;2:50 pubmed publisher
    ..These results suggest a functional relationship between the E6*I and full-length E6 protein which correlates with their localization and likely is important in regulation of the E6-E6AP complex...
  78. Bellanger S, Blachon S, Mechali F, Bonne Andrea C, Thierry F. High-risk but not low-risk HPV E2 proteins bind to the APC activators Cdh1 and Cdc20 and cause genomic instability. Cell Cycle. 2005;4:1608-15 pubmed publisher
    ..The finding that high-risk, but not low-risk HPV E2 proteins, induce genomic instability, raises the intriguing possibility that E2 proteins play a role in the oncogenic potential of high-risk papillomaviruses...
  79. Nguyen C, M nger K. Direct association of the HPV16 E7 oncoprotein with cyclin A/CDK2 and cyclin E/CDK2 complexes. Virology. 2008;380:21-5 pubmed publisher
    ..Moreover, we show that HPV16 E7 can directly associate with cyclin A/CDK2 and cyclin E/CDK2 complexes...
  80. Baldwin A, Li W, Grace M, Pearlberg J, Harlow E, M nger K, et al. Kinase requirements in human cells: II. Genetic interaction screens identify kinase requirements following HPV16 E7 expression in cancer cells. Proc Natl Acad Sci U S A. 2008;105:16478-83 pubmed publisher
    ..To identify kinases targeted by the HPV16 E7 oncoprotein, shRNA kinase screens were performed in RKO colorectal carcinoma cell lines that differ only in ..
  81. Yew C, Lee P, Chan W, Lim V, Tay S, Tan T, et al. A novel MLL5 isoform that is essential to activate E6 and E7 transcription in HPV16/18-associated cervical cancers. Cancer Res. 2011;71:6696-707 pubmed publisher
    ..MLL5? is present in HPV16/18-positive cells including human primary cervical carcinoma specimens...
  82. Zanier K, ould M hamed ould Sidi A, Boulade Ladame C, Rybin V, Chappelle A, Atkinson A, et al. Solution structure analysis of the HPV16 E6 oncoprotein reveals a self-association mechanism required for E6-mediated degradation of p53. Structure. 2012;20:604-17 pubmed publisher
    ..This allowed us to raise structural data covering the entire HPV16 E6 protein, including the high-resolution NMR structures of the two zinc-binding domains of E6 and a robust data-..
  83. Schneider M, Scheffer K, Bund T, Boukhallouk F, Lambert C, Cotarelo C, et al. The transcription factors TBX2 and TBX3 interact with human papillomavirus 16 (HPV16) L2 and repress the long control region of HPVs. J Virol. 2013;87:4461-74 pubmed publisher
    ..two-hybrid screening, we identified the transcription factor TBX2 as a novel interaction partner of HPV type 16 (HPV16) L2...
  84. Miller J, Dakic A, Chen R, Palechor Ceron N, Dai Y, Kallakury B, et al. HPV16 E7 protein and hTERT proteins defective for telomere maintenance cooperate to immortalize human keratinocytes. PLoS Pathog. 2013;9:e1003284 pubmed publisher
    ..that wild-type human telomerase reverse transcriptase (hTERT) protein can functionally replace the human papillomavirus type 16 (HPV-16) E6 protein, which cooperates with the viral E7 protein in the immortalization of primary ..
  85. Mazumder Indra D, Singh R, Mitra S, Dutta S, Chakraborty C, Basu P, et al. Genetic and epigenetic changes of HPV16 in cervical cancer differentially regulate E6/E7 expression and associate with disease progression. Gynecol Oncol. 2011;123:597-604 pubmed publisher
    The study was aimed at understanding the complex interactions of genetic and epigenetic events in expression of HPV16 E6/E7 and progression of cervical carcinoma...
  86. Conway M, Alam S, Christensen N, Meyers C. Overlapping and independent structural roles for human papillomavirus type 16 L2 conserved cysteines. Virology. 2009;393:295-303 pubmed publisher
    Cryoelectron microscopy images of HPV16 pseudovirions (PsV) depict that each pentamer of L1 can be occluded with a monomer of L2...
  87. Accardi R, Rubino R, Scalise M, Gheit T, Shahzad N, Thomas M, et al. E6 and E7 from human papillomavirus type 16 cooperate to target the PDZ protein Na/H exchange regulatory factor 1. J Virol. 2011;85:8208-16 pubmed publisher
    ..Here, we describe a novel interaction of HPV type 16 (HPV16) E6 with a PDZ protein, Na(+)/H(+) exchange regulatory factor 1 (NHERF-1), which is involved in a number of ..
  88. Greco D, Kivi N, Qian K, Leivonen S, Auvinen P, Auvinen E. Human papillomavirus 16 E5 modulates the expression of host microRNAs. PLoS ONE. 2011;6:e21646 pubmed publisher
    ..Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E5 protein. ..
  89. Ronco L, Karpova A, Vidal M, Howley P. Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. Genes Dev. 1998;12:2061-72 pubmed
    Interferon regulatory factor-3 (IRF-3) was found to specifically interact with HPV16 E6 in a yeast two-hybrid screen...
  90. Fiedler M, Muller Holzner E, Viertler H, Widschwendter A, Laich A, Pfister G, et al. High level HPV-16 E7 oncoprotein expression correlates with reduced pRb-levels in cervical biopsies. FASEB J. 2004;18:1120-2 pubmed
    ..We show here that pRb expression is initially upregulated in LSIL and disappears in later stages concomitant with increased E7 levels, suggesting that E7-driven degradation of pRb is involved in cervical tumorigenesis in humans. ..
  91. Xue Y, Bellanger S, Zhang W, Lim D, Low J, Lunny D, et al. HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma. Cancer Res. 2010;70:5316-25 pubmed publisher
    ..Using new polyclonal antibodies against the HPV16 E2 protein, we showed that E2 is expressed at various precursor stages of cervical carcinoma by ..
  92. Zanier K, Charbonnier S, Sidi A, McEwen A, Ferrario M, Poussin Courmontagne P, et al. Structural basis for hijacking of cellular LxxLL motifs by papillomavirus E6 oncoproteins. Science. 2013;339:694-8 pubmed publisher
    ..We solved the crystal structures of bovine (BPV1) and human (HPV16) papillomavirus E6 proteins bound to LxxLL peptides from the focal adhesion protein paxillin and the ubiquitin ..