Sulfolobus solfataricus P2

Summary

Alias: Sulfolobus solfataricus str. P2, Sulfolobus solfataricus strain P2

Top Publications

  1. Rechkoblit O, Malinina L, Cheng Y, Kuryavyi V, Broyde S, Geacintov N, et al. Stepwise translocation of Dpo4 polymerase during error-free bypass of an oxoG lesion. PLoS Biol. 2006;4:e11 pubmed
  2. Ling H, Boudsocq F, Plosky B, Woodgate R, Yang W. Replication of a cis-syn thymine dimer at atomic resolution. Nature. 2003;424:1083-7 pubmed
    ..A model of the pol eta-CPD complex built from the crystal structures of Saccharomyces cerevisiae apo-pol eta and the Dpo4-CPD complex suggests unique features that allow pol eta to efficiently bypass CPDs. ..
  3. Bell S, Botting C, Wardleworth B, Jackson S, White M. The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylation. Science. 2002;296:148-51 pubmed
    ..These data provide a paradigm for how Sir2 family proteins influence transcription and suggest that modulation of chromatin structure by acetylation arose before the divergence of the archaeal and eukaryotic lineages. ..
  4. Brewster A, Wang G, Yu X, Greenleaf W, Carazo J, Tjajadi M, et al. Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase. Proc Natl Acad Sci U S A. 2008;105:20191-6 pubmed publisher
    ..These structural and biochemical results provide a basis for future study of the helicase mechanisms of the archaeal and eukaryotic MCM complexes in DNA replication. ..
  5. Kerr I, Wadsworth R, Cubeddu L, Blankenfeldt W, Naismith J, White M. Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein. EMBO J. 2003;22:2561-70 pubmed
    ..The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds. ..
  6. Gao Y, Su S, Robinson H, Padmanabhan S, Lim L, McCrary B, et al. The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA. Nat Struct Biol. 1998;5:782-6 pubmed
    ..The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing. ..
  7. Marinsek N, Barry E, Makarova K, Dionne I, Koonin E, Bell S. GINS, a central nexus in the archaeal DNA replication fork. EMBO Rep. 2006;7:539-45 pubmed
    ..Our data suggest that GINS is important in coordinating the architecture of the replication fork and provide a mechanism to couple progression of the MCM helicase on the leading strand with priming events on the lagging strand. ..
  8. Roppelt V, Klug G, Evguenieva Hackenberg E. The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome. FEBS Lett. 2010;584:2931-6 pubmed publisher
    ..High C-content and polyuridylation negatively influence RNA processing by all complexes, and, in contrast to the hexamer, the Rrp4-exosome prefers longer substrates. ..
  9. Napoli A, van der Oost J, Sensen C, Charlebois R, Rossi M, Ciaramella M. An Lrp-like protein of the hyperthermophilic archaeon Sulfolobus solfataricus which binds to its own promoter. J Bacteriol. 1999;181:1474-80 pubmed
    ..coli Lrp, Lrs14 is autoregulated. We also show that the lrs14 transcript is accumulated in the late growth stages of S. solfataricus. ..

More Information

Publications81

  1. Walter P, Klein F, Lorentzen E, Ilchmann A, Klug G, Evguenieva Hackenberg E. Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2006;62:1076-89 pubmed
    ..Rrp4 and Csl4 do not exhibit any hydrolytic RNase activity, either when assayed alone or in context of the complex, but influence the activity of the archaeal exosome. ..
  2. Wang Y, Arora K, Schlick T. Subtle but variable conformational rearrangements in the replication cycle of Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) may accommodate lesion bypass. Protein Sci. 2006;15:135-51 pubmed
  3. Ling H, Boudsocq F, Woodgate R, Yang W. Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Cell. 2001;107:91-102 pubmed
    b>Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a DinB homolog that belongs to the recently described Y-family of DNA polymerases, which are best characterized by their low-fidelity synthesis on undamaged DNA templates and ..
  4. Xue H, Guo R, Wen Y, Liu D, Huang L. An abundant DNA binding protein from the hyperthermophilic archaeon Sulfolobus shibatae affects DNA supercoiling in a temperature-dependent fashion. J Bacteriol. 2000;182:3929-33 pubmed
    ..Taken together, our data suggest that archaeal proteins of the Sac10b family may affect the topology of chromosomal DNA in thermophilic archaea at their growth temperatures. ..
  5. Moreau M, McGeoch A, Lowe A, Itzhaki L, Bell S. ATPase site architecture and helicase mechanism of an archaeal MCM. Mol Cell. 2007;28:304-14 pubmed
    ..Our results indicate that MCM can tolerate catalytically inactive subunits and still function as a helicase, leading us to propose a semisequential model for helicase activity of this complex. ..
  6. Oke M, Carter L, Johnson K, Liu H, McMahon S, Yan X, et al. The Scottish Structural Proteomics Facility: targets, methods and outputs. J Struct Funct Genomics. 2010;11:167-80 pubmed publisher
    ..By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology. ..
  7. Parker J, White M. The endonuclease Hje catalyses rapid, multiple turnover resolution of Holliday junctions. J Mol Biol. 2005;350:1-6 pubmed
    ..These properties, coupled with a high level of specificity for four-way junctions over all other DNA substrates, make Hje a suitable enzyme for applications requiring the detection and cleavage of Holliday junctions in vitro. ..
  8. Choi J, Eoff R, Pence M, Wang J, Martin M, Kim E, et al. Roles of the four DNA polymerases of the crenarchaeon Sulfolobus solfataricus and accessory proteins in DNA replication. J Biol Chem. 2011;286:31180-93 pubmed publisher
    The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts...
  9. Colombo S, D Auria S, Fusi P, Zecca L, Raia C, Tortora P. Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus. Eur J Biochem. 1992;206:349-57 pubmed
    ..Amino acid and N-terminal sequence analyses did not evidence any similarity to carboxypeptidases A nor thermolysin. A weak similarity was only found with bovine carboxypeptidase B. ..
  10. Ling H, Boudsocq F, Woodgate R, Yang W. Snapshots of replication through an abasic lesion; structural basis for base substitutions and frameshifts. Mol Cell. 2004;13:751-62 pubmed
    ..In addition, the structure of an unproductive Dpo4 ternary complex suggests that the flexible little finger domain facilitates DNA orientation and translocation during translesion synthesis. ..
  11. Lorentzen E, Conti E. Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core. Mol Cell. 2005;20:473-81 pubmed
    ..The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome. ..
  12. Corbett K, Berger J. Structural dissection of ATP turnover in the prototypical GHL ATPase TopoVI. Structure. 2005;13:873-82 pubmed
    ..Our results also suggest that the GHL ATPase module is a molecular switch in which ATP hydrolysis serves as a prerequisite but not a driving force for substrate-dependent structural transitions in the enzyme. ..
  13. Lao Sirieix S, Bell S. The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities. J Mol Biol. 2004;344:1251-63 pubmed
    ..In addition to the primase and polymerase activities, we reveal that the primase possesses a template-independent 3'-terminal nucleotidyl transferase activity. ..
  14. Ariza A, Richard D, White M, Bond C. Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA. Nucleic Acids Res. 2005;33:1465-73 pubmed
    ..Additional analysis reveals that the interaction specificity of the five human Rad51 paralogues can be predicted using a simple model based on the RadA structure. ..
  15. Yatime L, Mechulam Y, Blanquet S, Schmitt E. Structural switch of the gamma subunit in an archaeal aIF2 alpha gamma heterodimer. Structure. 2006;14:119-28 pubmed
    ..Because the alpha subunit markedly reinforces the affinity of tRNA for the gamma subunit, a contribution of the alpha subunit to the switch movements observed in the gamma structure is considered. ..
  16. Eoff R, Irimia A, Egli M, Guengerich F. Sulfolobus solfataricus DNA polymerase Dpo4 is partially inhibited by "wobble" pairing between O6-methylguanine and cytosine, but accurate bypass is preferred. J Biol Chem. 2007;282:1456-67 pubmed
    ..The more stabilized C:O6-MeG pairing reinforces the importance of hydrogen bonding with respect to nucleotide selection within a geometrically tolerant polymerase active site. ..
  17. Carpentieri F, De Felice M, De Falco M, Rossi M, Pisani F. Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus. J Biol Chem. 2002;277:12118-27 pubmed
  18. Valenti A, Perugino G, Nohmi T, Rossi M, Ciaramella M. Inhibition of translesion DNA polymerase by archaeal reverse gyrase. Nucleic Acids Res. 2009;37:4287-95 pubmed publisher
    ..Inhibition of a translesion polymerase by topoisomerase-induced modification of DNA structure may represent a previously unconsidered mechanism of regulation of these two-faced enzymes. ..
  19. Lintner N, Kerou M, Brumfield S, Graham S, Liu H, Naismith J, et al. Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE). J Biol Chem. 2011;286:21643-56 pubmed publisher
    ..Conserved residues indicate potential crRNA- and target DNA-binding sites, and the H160A variant shows significantly reduced affinity for crRNA. We propose a general subunit architecture for CASCADE in other bacteria and Archaea. ..
  20. Wu K, Lai X, Guo X, Hu J, Xiang X, Huang L. Interplay between primase and replication factor C in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2007;63:826-37 pubmed
    ..These findings suggest that the primase-RFC interaction modulates the activities of both enzymes and therefore may be involved in the regulation of primer synthesis and the transfer of primers to DNA polymerase in Archaea. ..
  21. Lorentzen E, Walter P, Fribourg S, Evguenieva Hackenberg E, Klug G, Conti E. The archaeal exosome core is a hexameric ring structure with three catalytic subunits. Nat Struct Mol Biol. 2005;12:575-81 pubmed
    ..The high sequence similarity of this archaeal exosome to eukaryotic exosomes and its high structural similarity to the bacterial mRNA-degrading PNPase support a common basis for RNA-degrading machineries in all three domains of life. ..
  22. Eoff R, Irimia A, Angel K, Egli M, Guengerich F. Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2007;282:19831-43 pubmed
    b>Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly efficient and relatively accurate manner...
  23. Brenlla A, Markiewicz R, Rueda D, Romano L. Nucleotide selection by the Y-family DNA polymerase Dpo4 involves template translocation and misalignment. Nucleic Acids Res. 2014;42:2555-63 pubmed publisher
    ..Here, we have characterized the binding kinetics and conformational dynamics of the Y-family polymerase Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) using single-molecule fluorescence...
  24. Johnson R, Prakash L, Prakash S. Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase eta. Proc Natl Acad Sci U S A. 2005;102:12359-64 pubmed
    ..These observations have important implications for the mechanisms used for the inefficient vs. efficient bypass of CPDs by DNA Pols. ..
  25. Roberts J, Bell S, White M. An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA. Mol Microbiol. 2003;48:361-71 pubmed
    ..The PCNA-XPF complex acts as a structure-specific nuclease on a similar range of DNA flap, bubble and junction substrates as the human protein, suggesting a fundamental conservation through billions of years of evolution. ..
  26. Baumann H, Knapp S, Lundbäck T, Ladenstein R, Hard T. Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricus. Nat Struct Biol. 1994;1:808-19 pubmed
    ..In addition, we note that epsilon-mono-methylation of lysine side chains of Sso7d is governed by cell growth temperatures, suggesting that methylation is related to the heat-shock response. ..
  27. Dionne I, Bell S. Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins. Biochem J. 2005;387:859-63 pubmed
    ..However, analysis of the effects of Sulfolobus chromatin proteins on UDG1 leads us to propose a mechanistic basis for coupling UDG1 to the replication fork. ..
  28. Xing G, Kirouac K, Shin Y, Bell S, Ling H. Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA. Mol Microbiol. 2009;71:678-91 pubmed publisher
  29. Pisani F, De Martino C, Rossi M. A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases. Nucleic Acids Res. 1992;20:2711-6 pubmed
    ..coli DNA polymerase I and shared by various family A and B DNA polymerases, were also identified. This result suggests that the proofreading domains of all these enzymes are evolutionarily related. ..
  30. Evguenieva Hackenberg E, Roppelt V, Finsterseifer P, Klug G. Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome. Biochemistry. 2008;47:13158-68 pubmed publisher
    ..In summary, the data suggest that subunit composition and Mg(2+) are involved in the regulation of exosome activity. ..
  31. Zang H, Goodenough A, Choi J, Irimia A, Loukachevitch L, Kozekov I, et al. DNA adduct bypass polymerization by Sulfolobus solfataricus DNA polymerase Dpo4: analysis and crystal structures of multiple base pair substitution and frameshift products with the adduct 1,N2-ethenoguanine. J Biol Chem. 2005;280:29750-64 pubmed
    ..Gel electrophoretic analysis of the products of primer extension by Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) indicated preferential incorporation of A opposite 3'-(1,N(2)-epsilon-G)TACT-5',..
  32. Vaisman A, Ling H, Woodgate R, Yang W. Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis. EMBO J. 2005;24:2957-67 pubmed
    ..The correct incoming nucleotide allows DNA synthesis to overcome pyrophosphorolysis, but an incorrect incoming nucleotide does not. ..
  33. Agback P, Baumann H, Knapp S, Ladenstein R, Hard T. Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex. Nat Struct Biol. 1998;5:579-84 pubmed
    ..Sso7d binding also distorts the DNA conformation and introduces significant unwinding of the helix. This effect suggests a mechanism for DNA packing in Sulfolobus based on negative DNA supercoiling. ..
  34. Napoli A, Valenti A, Salerno V, Nadal M, Garnier F, Rossi M, et al. Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. Nucleic Acids Res. 2005;33:564-76 pubmed
    ..The results shown here suggest that these functional interactions may have biological relevance and that the interplay of different DNA binding proteins might modulate reverse gyrase activity in DNA metabolic pathways. ..
  35. Zang H, Irimia A, Choi J, Angel K, Loukachevitch L, Egli M, et al. Efficient and high fidelity incorporation of dCTP opposite 7,8-dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA polymerase Dpo4. J Biol Chem. 2006;281:2358-72 pubmed
    ..With dGTP placed opposite 8-oxoG, pairing was not to the 8-oxoG but to the 5' C (and in classic Watson-Crick geometry), consistent with the low frequency of this frameshift event observed in the catalytic assays. ..
  36. Corbett K, Berger J. Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution. EMBO J. 2003;22:151-63 pubmed
    ..When combined with the structure of the A subunit to create a picture of the intact topoisomerase VI holoenzyme, the ATP-driven motions of topoVI-B reveal a simple mechanism for strand passage by the type IIB topoisomerases. ..
  37. Bergerat A, de Massy B, Gadelle D, Varoutas P, Nicolas A, Forterre P. An atypical topoisomerase II from Archaea with implications for meiotic recombination. Nature. 1997;386:414-7 pubmed
    ..By analogy with the mechanism of action of known type II topoisomerases, we suggest that Spo11 catalyses the formation of double-strand breaks that initiate meiotic recombination in S. cerevisiae. ..
  38. Brinkman A, Bell S, Lebbink R, de Vos W, van der Oost J. The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability. J Biol Chem. 2002;277:29537-49 pubmed
    ..As with transcriptional activation by Escherichia coli Lrp, activation by LysM is apparently dependent on a co-activator, which remains to be identified. ..
  39. Wong J, Fiala K, Suo Z, Ling H. Snapshots of a Y-family DNA polymerase in replication: substrate-induced conformational transitions and implications for fidelity of Dpo4. J Mol Biol. 2008;379:317-30 pubmed publisher
    ..Together, the global and local conformational transitions observed in our study provide a structural basis for the distinct kinetic steps of a catalytic cycle of DNA polymerization performed by a Y-family polymerase. ..
  40. Kokoska R, Bebenek K, Boudsocq F, Woodgate R, Kunkel T. Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. J Biol Chem. 2002;277:19633-8 pubmed
    ..These results have implications for mutagenesis resulting from DNA synthesis by Y family polymerases. ..
  41. Pucci B, De Felice M, Rossi M, Onesti S, Pisani F. Amino acids of the Sulfolobus solfataricus mini-chromosome maintenance-like DNA helicase involved in DNA binding/remodeling. J Biol Chem. 2004;279:49222-8 pubmed
    ..These results have important implications for the understanding of the molecular mechanism of the MCM DNA helicase action...
  42. Eoff R, Stafford J, Szekely J, Rizzo C, Egli M, Guengerich F, et al. Structural and functional analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed bypass of the malondialdehyde-deoxyguanosine adduct. Biochemistry. 2009;48:7079-88 pubmed publisher
    ..The results are consistent with the reported mutagenicity of M1dG and illustrate how the lesion may affect replication events...
  43. Wang J, Jiang P, Feng H, Feng Y, He Z. Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2. Biochem Biophys Res Commun. 2007;363:63-70 pubmed publisher
    ..This is the first report on the functional interaction among the archaeal multiple Cdc6 proteins to regulate DNA replication...
  44. Han D, Krauss G. Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus P2. FEBS Lett. 2009;583:771-6 pubmed publisher
    ..Point mutations identify both highly conserved aspartate and glutamate residues as being crucial for the nuclease activity. The catalytic activity shows an optimum at neutral pH and pH 3...
  45. Wardleworth B, Russell R, Bell S, Taylor G, White M. Structure of Alba: an archaeal chromatin protein modulated by acetylation. EMBO J. 2002;21:4654-62 pubmed
    ..We present a model for the Alba-DNA interaction consistent with the available structural, biophysical and electron microscopy data...
  46. Mai V, Chen X, Hong R, Huang L. Small abundant DNA binding proteins from the thermoacidophilic archaeon Sulfolobus shibatae constrain negative DNA supercoils. J Bacteriol. 1998;180:2560-3 pubmed
    ..Our results, together with the observation that the viral DNA isolated from S. shibatae is relaxed, suggest that regions of free DNA in the S. shibatae genome, if present, are highly positively supercoiled...
  47. Witharana C, Roppelt V, Lochnit G, Klug G, Evguenieva Hackenberg E. Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus. Biochimie. 2012;94:1578-87 pubmed publisher
    ..Although the deletion of the KH domain negatively influenced the degradation activity of the exosome, the poly(A) specificity was retained, showing that the KH domain is dispensable for the strong poly(A) preference of Rrp4...
  48. Han D, Lehmann K, Krauss G. SSO1450--a CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA. FEBS Lett. 2009;583:1928-32 pubmed publisher
    ..It binds DNA, RNA and DNA-RNA hybrid apparently sequence non-specific in a multi-site binding mode. Furthermore, SSO1450 promotes the hybridization of complementary nucleic acid strands...
  49. Schmitt E, Panvert M, Lazennec Schurdevin C, Coureux P, Perez J, Thompson A, et al. Structure of the ternary initiation complex aIF2-GDPNP-methionylated initiator tRNA. Nat Struct Mol Biol. 2012;19:450-4 pubmed publisher
    ..We conclude that despite considerable structural homology between the core ? subunit of aIF2 and the elongation factor EF1A, these two G proteins of the translation apparatus use very different tRNA-binding strategies...
  50. Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, et al. Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity. Mol Cell. 2012;45:303-13 pubmed publisher
    ..Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets. ..
  51. Banerjee S, Brown K, Egli M, Stone M. Bypass of aflatoxin B1 adducts by the Sulfolobus solfataricus DNA polymerase IV. J Am Chem Soc. 2011;133:12556-68 pubmed publisher
    ..We show that the Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) bypasses AFB(1)-N7-dG in an error-free manner but conducts error-prone ..
  52. Zhang H, Guengerich F. Effect of N2-guanyl modifications on early steps in catalysis of polymerization by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W. J Mol Biol. 2010;395:1007-18 pubmed publisher
    ..With the larger adducts (>or=(2-naphthyl)methyl), the absence of fluorescence changes suggests impaired ability to undergo an appropriate conformational change, consistent with previous structural work...
  53. Lorentzen E, Dziembowski A, Lindner D, Seraphin B, Conti E. RNA channelling by the archaeal exosome. EMBO Rep. 2007;8:470-6 pubmed
    ..Multiple substrate-binding sites and the entrapment of the substrate in the central channel provide a rationale for the processive degradation of extended RNAs and the stalling of structured RNAs...
  54. Eoff R, Angel K, Egli M, Guengerich F. Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. J Biol Chem. 2007;282:13573-84 pubmed
  55. Chen L, Ko T, Chang Y, Lin K, Chang C, Wang A, et al. Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins. Nucleic Acids Res. 2007;35:1787-801 pubmed
    ..These observations support the hypothesis that RecA family protein filaments may function as rotary motors...
  56. Fiala K, Hypes C, Suo Z. Mechanism of abasic lesion bypass catalyzed by a Y-family DNA polymerase. J Biol Chem. 2007;282:8188-98 pubmed
    ..Our results suggest that abasic lesion bypass requires tight regulation to maintain genomic stability...
  57. Evguenieva Hackenberg E, Walter P, Hochleitner E, Lottspeich F, Klug G. An exosome-like complex in Sulfolobus solfataricus. EMBO Rep. 2003;4:889-93 pubmed
    ..Such a protein complex was probably present in the last common ancestor of Archaea and Eukarya...
  58. Jiang P, Wang J, Feng Y, He Z. Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2. Biochem Biophys Res Commun. 2007;361:651-8 pubmed publisher
    ..This is the first report on the divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on regulating the loading of the MCM helicase onto the origins in the archaeon...
  59. Beloglazova N, Brown G, Zimmerman M, Proudfoot M, Makarova K, Kudritska M, et al. A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J Biol Chem. 2008;283:20361-71 pubmed publisher
    ..Thus, CAS2 proteins are sequence-specific endoribonucleases, and we propose that their role in the CRISPR-mediated anti-phage defense might involve degradation of phage or cellular mRNAs...
  60. Richards J, Johnson K, Liu H, McRobbie A, McMahon S, Oke M, et al. Structure of the DNA repair helicase hel308 reveals DNA binding and autoinhibitory domains. J Biol Chem. 2008;283:5118-26 pubmed publisher
    ..These data suggest that one function of the enzyme may be in the removal of bound proteins at stalled replication forks and recombination intermediates...
  61. Williams G, Johnson K, Rudolf J, McMahon S, Carter L, Oke M, et al. Structure of the heterotrimeric PCNA from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:944-8 pubmed publisher
    ..The resulting structure to 2.3 A sheds light on the differential stabilities of the interactions observed between the three subunits and the specificity of individual subunits for partner proteins...
  62. Jaxel C, Bouthier de la Tour C, Duguet M, Nadal M. Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domains. Nucleic Acids Res. 1996;24:4668-75 pubmed
  63. Sandler S, Satin L, Samra H, Clark A. recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae. Nucleic Acids Res. 1996;24:2125-32 pubmed
    ..coli and S.cerevisiae. Hence it is likely that radA in this organism is a constitutively expressed gene and we discuss possible implications of the lack of UV-inducibility...
  64. Guo R, Xue H, Huang L. Ssh10b, a conserved thermophilic archaeal protein, binds RNA in vivo. Mol Microbiol. 2003;50:1605-15 pubmed
    ..Our data indicate that RNA is the physiological binding target of the Sac10b family...
  65. Lao Sirieix S, Nookala R, Roversi P, Bell S, Pellegrini L. Structure of the heterodimeric core primase. Nat Struct Mol Biol. 2005;12:1137-44 pubmed publisher
    ..Modeling of a DNA-RNA helix together with structure-based site-directed mutagenesis provides insight into the mechanism of template DNA binding and RNA primer synthesis...
  66. Trincao J, Johnson R, Wolfle W, Escalante C, Prakash S, Prakash L, et al. Dpo4 is hindered in extending a G.T mismatch by a reverse wobble. Nat Struct Mol Biol. 2004;11:457-62 pubmed publisher
    ..The structures offer a basis for the low efficiency of Dpo4 in extending a G.T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide...
  67. Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M. Conformational transitions in the ? subunit of the archaeal translation initiation factor 2. Acta Crystallogr D Biol Crystallogr. 2014;70:658-67 pubmed publisher
    ..The structures describe the different states of aIF2? and demonstrate the conformational transitions that take place in the aIF2? `life cycle'...
  68. Ling H, Sayer J, Plosky B, Yagi H, Boudsocq F, Woodgate R, et al. Crystal structure of a benzo[a]pyrene diol epoxide adduct in a ternary complex with a DNA polymerase. Proc Natl Acad Sci U S A. 2004;101:2265-9 pubmed
    ..These structures also suggest a mechanism by which mutations are generated during replication of DNA containing BPDE adducts. ..
  69. Boudsocq F, Iwai S, Hanaoka F, Woodgate R. Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Nucleic Acids Res. 2001;29:4607-16 pubmed
    ..Searches of the complete genome of the crenarchaeon Sulfolobus solfataricus P2 reveal that it possesses a DinB homolog that has been termed DNA polymerase IV (Dpo4)...
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    The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner...