Sulfolobus acidocaldarius

Summary

Alias: Sulfolobus acidocaldarius Brock et al. 1972

Top Publications

  1. Lin X, Tang J. Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius. J Biol Chem. 1990;265:1490-5 pubmed
    ..acid proteolytic activity has been found to be associated with the cells and in the culture medium of Sulfolobus acidocaldarius, an archaebacterium...
  2. P hler G, Lottspeich F, Zillig W. Organization and nucleotide sequence of the genes encoding the large subunits A, B and C of the DNA-dependent RNA polymerase of the archaebacterium Sulfolobus acidocaldarius. Nucleic Acids Res. 1989;17:4517-34 pubmed
    ..for the three large subunits A, B and C, of the DNA-dependent RNA polymerase of the archaebacterium Sulfolobus acidocaldarius DSM 639, were identified and characterized...
  3. Arpigny J, Jendrossek D, Jaeger K. A novel heat-stable lipolytic enzyme from Sulfolobus acidocaldarius DSM 639 displaying similarity to polyhydroxyalkanoate depolymerases. FEMS Microbiol Lett. 1998;167:69-73 pubmed
    A fragment of genomic DNA from Sulfolobus acidocaldarius DSM 639 encoding a lipolytic enzyme was cloned and sequenced...
  4. McAfee J, Edmondson S, Datta P, Shriver J, Gupta R. Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius. Biochemistry. 1995;34:10063-77 pubmed
    The genes for two Sac7 DNA-binding proteins, Sac7d and Sac7e, from the extremely thermophilic archaeon Sulfolobus acidocaldarius have been cloned into Escherichia coli and sequenced...
  5. L bben M, Arnaud S, Castresana J, Warne A, Albracht S, Saraste M. A second terminal oxidase in Sulfolobus acidocaldarius. Eur J Biochem. 1994;224:151-9 pubmed
    We previously found that the soxABCD operon encodes a quinol oxidase complex in Sulfolobus acidocaldarius and this enzyme was purified and characterized...
  6. Hettmann T, Schmidt C, Anem ller S, Z hringer U, Moll H, Petersen A, et al. Cytochrome b558/566 from the archaeon Sulfolobus acidocaldarius. A novel highly glycosylated, membrane-bound b-type hemoprotein. J Biol Chem. 1998;273:12032-40 pubmed
    In this study we re-examined the inducible cytochrome b558/566 from the archaeon Sulfolobus acidocaldarius (DSM 639), formerly thought to be a component of a terminal oxidase (Becker, M., and Schäfer, G. (1991) FEBS Lett. 291, 331-335)...
  7. Janssen S, Sch fer G, Anem ller S, Moll R. A succinate dehydrogenase with novel structure and properties from the hyperthermophilic archaeon Sulfolobus acidocaldarius: genetic and biophysical characterization. J Bacteriol. 1997;179:5560-9 pubmed
    The sdh operon of Sulfolobus acidocaldarius DSM 639 is composed of four genes coding for the 63.1-kDa flavoprotein (SdhA), the 36.5-kDa iron-sulfur protein (SdhB), and the 32.1-kDa SdhC and 14.1-kDa SdhD subunits...
  8. Maruta K, Mitsuzumi H, Nakada T, Kubota M, Chaen H, Fukuda S, et al. Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius. Biochim Biophys Acta. 1996;1291:177-81 pubmed
    ..and maltooligosyltrehalose synthase (TreY) have been cloned from the thermophilic archaebacterium Sulfolobus acidocaldarius ATCC33909...
  9. Lampe J, Brandman R, Sivaramakrishnan S, de Montellano P. Two-dimensional NMR and all-atom molecular dynamics of cytochrome P450 CYP119 reveal hidden conformational substates. J Biol Chem. 2010;285:9594-603 pubmed publisher
    ..molecular dynamics simulations to examine substrate and inhibitor binding to CYP119, a P450 from Sulfolobus acidocaldarius. The results suggest that tight binding hydrophobic ligands tend to lock the enzyme into a single ..

More Information

Publications73

  1. Su L, Wu S, Feng J, Wu J. High-efficiency expression of Sulfolobus acidocaldarius maltooligosyl trehalose trehalohydrolase in Escherichia coli through host strain and induction strategy optimization. Bioprocess Biosyst Eng. 2019;42:345-354 pubmed publisher
    ..In this study, a codon-optimized synthetic gene encoding Sulfolobus acidocaldarius MTHase was expressed in Escherichia coli...
  2. Buddeweg A, Daume M, Randau L, Schmitz R. Noncoding RNAs in Archaea: Genome-Wide Identification and Functional Classification. Methods Enzymol. 2018;612:413-442 pubmed publisher
    ..In addition, details of applying RNA-seq methodology for the detection of ncRNAs in Sulfolobus acidocaldarius are provided...
  3. Zeng Z, Liu X, Wei J, Summons R, Welander P. Calditol-linked membrane lipids are required for acid tolerance in Sulfolobus acidocaldarius. Proc Natl Acad Sci U S A. 2018;115:12932-12937 pubmed publisher
    ..In this study, we identify a radical S-adenosylmethionine (SAM) protein in Sulfolobus acidocaldarius required for the synthesis of a unique cyclopentyl head group, known as calditol...
  4. Park J, Lee A, Lee H, Park I, Seo Y, Cha J. Profiling of glucose-induced transcription in Sulfolobus acidocaldarius DSM 639. Genes Genomics. 2018;40:1157-1167 pubmed publisher
    ..The present study revealed metabolic networks in S. acidocaldarius that are induced in a glucose-dependent manner, improving our understanding of biomass production under sugar-rich conditions. ..
  5. Roy M, Gupta S, Patranabis S, Ghosh A. The oligomeric plasticity of Hsp20 of Sulfolobus acidocaldarius protects environment-induced protein aggregation and membrane destabilization. Biochim Biophys Acta Biomembr. 2018;1860:2549-2565 pubmed publisher
    ..we investigated the structural and functional properties of Hsp20 from thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. To provide a framework for investigating the structure-function relationship of Hsp20 and understanding ..
  6. Porzio E, Bettazzi F, Mandrich L, Del Giudice I, Restaino O, Laschi S, et al. Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents. Sci Rep. 2018;8:13773 pubmed publisher
    ..acidocaldarius and of phosphotriesterase-like lactonases from Sulfolobus solfataricus and Sulfolobus acidocaldarius, have been selected and assembled in an optimized format for the development of an electrochemical ..
  7. Gietl A, Holzmeister P, Blombach F, Schulz S, von Voithenberg L, Lamb D, et al. Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways. Nucleic Acids Res. 2014;42:6219-31 pubmed publisher
    ..In contrast, the archaeal TBP-DNA interaction is extremely dynamic and TBP from the archaeal organism Sulfolobus acidocaldarius strictly requires TFB for DNA bending...
  8. Chen C, Ko T, Lin K, Lin B, Huang C, Chiang C, et al. NADH/NADPH bi-cofactor-utilizing and thermoactive ketol-acid reductoisomerase from Sulfolobus acidocaldarius. Sci Rep. 2018;8:7176 pubmed publisher
    ..Here, we characterize a thermoacidophilic archaeal Sac-KARI from Sulfolobus acidocaldarius and present its crystal structure at a 1.75-Å resolution...
  9. Sholder G, Loechler E. A method to accurately quantitate intensities of (32)P-DNA bands when multiple bands appear in a single lane of a gel is used to study dNTP insertion opposite a benzo[a]pyrene-dG adduct by Sulfolobus DNA polymerases Dpo4 and Dbh. DNA Repair (Amst). 2015;25:97-103 pubmed publisher
    ..opposite a benzo[a]pyrene-N(2)-dG-adduct with four DNAPs, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh...
  10. Sakai H, Kurosawa N. Saccharolobus caldissimus gen. nov., sp. nov., a facultatively anaerobic iron-reducing hyperthermophilic archaeon isolated from an acidic terrestrial hot spring, and reclassification of Sulfolobus solfataricus as Saccharolobus solfataricus comb. nov. Int J Syst Evol Microbiol. 2018;68:1271-1278 pubmed publisher
    ..However, the sequence similarity to the type species of the genus Sulfolobus (Sulfolobus acidocaldarius) was remarkably low (91.8 %)...
  11. Meyer B, Birich A, Albers S. N-Glycosylation of the archaellum filament is not important for archaella assembly and motility, although N-Glycosylation is essential for motility in Sulfolobus acidocaldarius. Biochimie. 2015;118:294-301 pubmed publisher
    ..In this study we demonstrate that the archaellum filament subunit FlaB of Sulfolobus acidocaldarius is N-glycosylated...
  12. Reitz T, Rossberg A, Barkleit A, Steudtner R, Selenska Pobell S, Merroun M. Spectroscopic study on uranyl carboxylate complexes formed at the surface layer of Sulfolobus acidocaldarius. Dalton Trans. 2015;44:2684-92 pubmed publisher
    The complexation of U(vi) at the proteinaceous surface layer (S-layer) of the archaeal strain Sulfolobus acidocaldarius was investigated over a pH range from pH 1...
  13. Restaino O, Borzacchiello M, Scognamiglio I, Fedele L, Alfano A, Porzio E, et al. High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers. BMC Biotechnol. 2018;18:18 pubmed publisher
    ..coli, the wild type SacPox from Sulfolobus acidocaldarius and a triple mutated SsoPox C258L/I261F/W263A, originally from Sulfolobus solfataricus...
  14. Moon J, Lee W, Park J, Choi K, Cha J. Characterization of a trehalose-degrading enzyme from the hyperthermophilic archaeon Sulfolobus acidocaldarius. J Biosci Bioeng. 2016;122:47-51 pubmed publisher
    We purified a cytosolic trehalase (TreH) from a thermoacidophilic archaeon Sulfolobus acidocaldarius. Enzyme activity in cell-free extracts indicated that trehalose degradation in the cell occurred via the hydrolytic activity of TreH, ..
  15. Banerjee A, Tsai C, Chaudhury P, Tripp P, Arvai A, Ishida J, et al. FlaF Is a β-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein. Structure. 2015;23:863-872 pubmed publisher
    ..In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J...
  16. Mahmoud G, Jedelská J, Strehlow B, Bakowsky U. Bipolar tetraether lipids derived from thermoacidophilic archaeon Sulfolobus acidocaldarius for membrane stabilization of chlorin e6 based liposomes for photodynamic therapy. Eur J Pharm Biopharm. 2015;95:88-98 pubmed publisher
    ..Tetraether lipids (TELs) derived from Sulfolobus acidocaldarius were added to DOTAP:Ce6 assembly in a concentration range of 2.5×10(-4)-1...
  17. Moro S, Cocco M. (1)H, (13)C, and (15)N backbone resonance assignments of the full-length 40 kDa S. acidocaldarius Y-family DNA polymerase, dinB homolog. Biomol NMR Assign. 2015;9:441-5 pubmed publisher
    ..the (15)N, (1)H, and (13)C backbone resonance assignments at two temperatures (35 and 50 °C) for Sulfolobus acidocaldarius Dbh polymerase. Backbone resonance assignments have been obtained for 86 % of the residues...
  18. Suzuki S, Kurosawa N. Disruption of the gene encoding restriction endonuclease SuaI and development of a host-vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius. Extremophiles. 2016;20:139-48 pubmed publisher
    b>Sulfolobus acidocaldarius is a useful model organism for the genetic study of thermophilic archaea due to its ease of cultivation. Here we describe the development of a host-vector system for S...
  19. Chaudhury P, Tripp P, Albers S. Expression, Purification, and Assembly of Archaellum Subcomplexes of Sulfolobus acidocaldarius. Methods Mol Biol. 2018;1764:307-314 pubmed publisher
    The archaellum assembly machinery and its filament consist of seven proteins in the crenarchaeon Sulfolobus acidocaldarius. We have so far expressed, purified, and biochemically characterized four of these archaellum subunits, namely, ..
  20. Yuasa M, Okamura T, Kimura M, Honda S, Shin Y, Kawakita M, et al. Two trehalose-hydrolyzing enzymes from Crenarchaeon Sulfolobus acidocaldarius exhibit distinct activities and affinities toward trehalose. Appl Microbiol Biotechnol. 2018;102:4445-4455 pubmed publisher
    ..and Saci1816, belonging to glycoside hydrolase family 15 (GH15) from the acidophilic Crenarchaeon Sulfolobus acidocaldarius were expressed in Escherichia coli...
  21. van Wolferen M, Ma X, Albers S. DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales. J Bacteriol. 2015;197:2941-51 pubmed publisher
    ..in the repair or transfer of DNA that takes place after UV-induced damage to the genomic DNA of Sulfolobus acidocaldarius. Sulfolobales species increase their fitness after UV stress by a UV-inducible pilus system that enables ..
  22. van Wolferen M, Wagner A, van der Does C, Albers S. The archaeal Ced system imports DNA. Proc Natl Acad Sci U S A. 2016;113:2496-501 pubmed publisher
    ..Here we identify saci_0568 and saci_0748, two genes from Sulfolobus acidocaldarius that are highly induced upon UV treatment, encoding a transmembrane protein and a membrane-bound VirB4/..
  23. Sakofsky C, Grogan D. Lesion-Induced Mutation in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius and Its Avoidance by the Y-Family DNA Polymerase Dbh. Genetics. 2015;201:513-23 pubmed publisher
    ..and used them to measure the efficiency and accuracy of bypass in normal and dbh(-) strains of Sulfolobus acidocaldarius. Oligonucleotide-mediated transformation allowed low levels of abasic-site bypass to be observed in S...
  24. Souza R, Cantão M, Nogueira M, Vasconcelos A, Hungria M. Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. Braz J Microbiol. 2018;49:723-730 pubmed publisher
    ..and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases...
  25. Pineda De Castro L, Dopson M, Friedman R. Biological Membranes in Extreme Conditions: Simulations of Anionic Archaeal Tetraether Lipid Membranes. PLoS ONE. 2016;11:e0155287 pubmed publisher
    ..Amber Force Field (GAFF) to enable the study of anionic tetraether membranes of the model archaean Sulfolobus acidocaldarius by computer simulations...
  26. Yi G, Wang W, Cao W, Wang F, Liu X. Sulfolobus acidocaldarius UDG Can Remove dU from the RNA Backbone: Insight into the Specific Recognition of Uracil Linked with Deoxyribose. Genes (Basel). 2017;8: pubmed publisher
    i>Sulfolobus acidocaldarius encodes family 4 and 5 uracil-DNA glycosylase (UDG). Two recombinant S. acidocaldarius UDGs (SacUDG) were prepared and biochemically characterized using oligonucleotides carrying a deaminated base...
  27. Meyer B, Shams Eldin H, Albers S. AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7. Extremophiles. 2017;21:121-134 pubmed publisher
    ..is initiating the protein N-glycosylation pathway in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. AglH successfully replaced the endogenous GlcNAc-1-phosphotransferase activity of Alg7 in a conditional ..
  28. Daume M, Uhl M, Backofen R, Randau L. RIP-Seq Suggests Translational Regulation by L7Ae in Archaea. MBio. 2017;8: pubmed publisher
    ..sequencing (RIP-Seq) analysis of genomically tagged L7Ae from the hyperthermophilic archaeon Sulfolobus acidocaldarius A large set of interacting noncoding RNAs was identified...
  29. Fuke T, Sato T, Jha S, Tansengco M, Atomi H. Phytoene production utilizing the isoprenoid biosynthesis capacity of Thermococcus kodakarensis. Extremophiles. 2018;22:301-313 pubmed publisher
    ..Therefore, a squalene/phytoene synthase homolog from an acidothermophilic archaeon Sulfolobus acidocaldarius, Saci_1734, was introduced into the T. kodakarensis chromosome under the control of a strong promoter...
  30. Liu H, Wang K, Lindås A, Peeters E. The genome-scale DNA-binding profile of BarR, a ?-alanine responsive transcription factor in the archaeon Sulfolobus acidocaldarius. BMC Genomics. 2016;17:569 pubmed publisher
    ..BarR is an Lrp-like transcription factor in the model archaeon Sulfolobus acidocaldarius that activates the expression of a ?-alanine aminotransferase gene, which is involved in ?-alanine ..
  31. Mao D, Grogan D. How a Genetically Stable Extremophile Evolves: Modes of Genome Diversification in the Archaeon Sulfolobus acidocaldarius. J Bacteriol. 2017;199: pubmed publisher
    ..polymorphisms (PMs) were identified in laboratory constructs and wild-type isolates, respectively, of Sulfolobus acidocaldarius Among wild-type isolates drawn from one local population, pairwise nucleotide divergence averaged 4 ..
  32. Uhl P, Helm F, Hofhaus G, Brings S, Kaufman C, Leotta K, et al. A liposomal formulation for the oral application of the investigational hepatitis B drug Myrcludex B. Eur J Pharm Biopharm. 2016;103:159-166 pubmed publisher
    ..For this purpose, tetraether lipids were extracted from the extremophilic archaeon Sulfolobus acidocaldarius and purified in order to obtain the desired glycerylcaldityltetraether lipids (GCTE)...
  33. Takahashi K, Nakanishi F, Tomita T, Akiyama N, Lassak K, Albers S, et al. Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius. Extremophiles. 2016;20:843-853 pubmed
    b>Sulfolobus acidocaldarius, a hyperthermoacidophilic archaeon, possesses two β-decarboxylating dehydrogenase genes, saci_0600 and saci_2375, in its genome, which suggests that it uses these enzymes for three similar reactions in lysine ..
  34. Dar D, Prasse D, Schmitz R, Sorek R. Widespread formation of alternative 3' UTR isoforms via transcription termination in archaea. Nat Microbiol. 2016;1:16143 pubmed publisher
    ..termini, with single-base precision, in two phylogenetically distant archaea: Methanosarcina mazei and Sulfolobus acidocaldarius. Comparison of RNA 3' ends across hundreds of genes revealed the sequence composition of transcriptional ..
  35. Ding Y, Vrionis H, Schneider J, Berezuk A, Khursigara C, Jarrell K. Complementation of an aglB Mutant of Methanococcus maripaludis with Heterologous Oligosaccharyltransferases. PLoS ONE. 2016;11:e0167611 pubmed publisher
    ..Attempts to use AglB from Methanocaldococcus jannaschii, Haloferax volcanii or Sulfolobus acidocaldarius to functionally replace the oligosaccharyltransferase activity missing in the Mc...
  36. Ener M, Gray H, Winkler J. Hole Hopping through Tryptophan in Cytochrome P450. Biochemistry. 2017;56:3531-3538 pubmed publisher
    ..The conjugates are constructed with enzymes from Bacillus megaterium (CYP102A1) and Sulfolobus acidocaldarius (CYP119)...
  37. Wang K, Lindås A. Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius. Methods Mol Biol. 2018;1689:139-146 pubmed publisher
    ..This chapter presents a stepwise protocol for ChIP performed in hyperthermophilic archaea that we have successfully used in the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius.
  38. Li L, Banerjee A, Bischof L, Maklad H, Hoffmann L, Henche A, et al. Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol. 2017;105:777-793 pubmed publisher
    ..Here, we study the Lrs14 member AbfR1 of Sulfolobus acidocaldarius. Small-angle X-ray scattering data are presented, which are consistent with a model of dimeric AbfR1 in ..
  39. Wagner M, Shen L, Albersmeier A, van der Kolk N, Kim S, Cha J, et al. Sulfolobus acidocaldarius Transports Pentoses via a Carbohydrate Uptake Transporter 2 (CUT2)-Type ABC Transporter and Metabolizes Them through the Aldolase-Independent Weimberg Pathway. Appl Environ Microbiol. 2018;84: pubmed publisher
    ..these transporters, the maltose/maltooligomer transporter, could be identified in the closely related Sulfolobus acidocaldarius Comparison of the transcriptome of S...
  40. Guan Z, Delago A, Nußbaum P, Meyer B, Albers S, Eichler J. Gene deletions leading to a reduction in the number of cyclopentane rings in Sulfolobus acidocaldarius tetraether lipids. FEMS Microbiol Lett. 2017;: pubmed publisher
    The cell membrane of (hyper)thermophilic archaea, including the thermoacidophile Sulfolobus acidocaldarius, incorporate dibiphytanylglycerol tetraether lipids...
  41. Mahmoud G, Jedelská J, Strehlow B, Omar S, Schneider M, Bakowsky U. Photo-responsive tetraether lipids based vesicles for prophyrin mediated vascular targeting and direct phototherapy. Colloids Surf B Biointerfaces. 2017;159:720-728 pubmed publisher
    Tetraether lipids (TELs) derived from the thermoacidophilic archaeon Sulfolobus acidocaldarius are dominated by polyisoprenoid skeleton...
  42. Engelhardt K, Pinnapireddy S, Baghdan E, Jedelská J, Bakowsky U. Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius. Archaea. 2017;2017:8047149 pubmed publisher
    ..In this study, two fractions of tetraether lipids from the archaea Sulfolobus acidocaldarius were extracted and purified using different methods...
  43. Guan Z, Delago A, Nußbaum P, Meyer B, Albers S, Eichler J. N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier. FEBS Lett. 2016;590:3168-78 pubmed publisher
    ..In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans ..
  44. Miot J, Bernard S, Bourreau M, Guyot F, Kish A. Experimental maturation of Archaea encrusted by Fe-phosphates. Sci Rep. 2017;7:16984 pubmed publisher
    ..For the present study, we submitted Sulfolobus acidocaldarius experimentally encrusted by amorphous Fe phosphates to constrained temperature conditions (150?°C) ..
  45. Suzuki S, Kurosawa N. Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius. Archaea. 2017;2017:7459310 pubmed publisher
    Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps...
  46. Moll R, Schmidtke S, Schaefer G. A putative signal recognition particle receptor alpha subunit (SR alpha) homologue is expressed in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius. FEMS Microbiol Lett. 1996;137:51-6 pubmed
    A 1.64 kb genomic DNA sequence from the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius is composed of two adjacent genes. The first functionally unassigned open reading frame (orf-1) comprises 450 base pairs. The second 1...
  47. L bben M, Kolmerer B, Saraste M. An archaebacterial terminal oxidase combines core structures of two mitochondrial respiratory complexes. EMBO J. 1992;11:805-12 pubmed
    The operon coding for a respiratory quinol oxidase was cloned from thermoacidophilic archaebacterium Sulfolobus acidocaldarius. It contains three genes, soxA, soxB and soxC...
  48. Castresana J, L bben M, Saraste M. New archaebacterial genes coding for redox proteins: implications for the evolution of aerobic metabolism. J Mol Biol. 1995;250:202-10 pubmed publisher
    ..We have cloned and sequenced a cluster of five new genes from the archaebacterium Sulfolobus acidocaldarius, four of them coding for redox proteins: a Rieske iron-sulphur protein, a cytochrome b, a subunit II of ..
  49. Langer D, Zillig W. Putative tfIIs gene of Sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of DNA-dependent RNA polymerase. Nucleic Acids Res. 1993;21:2251 pubmed
  50. Elie C, Baucher M, Fondrat C, Forterre P. A protein related to eucaryal and bacterial DNA-motor proteins in the hyperthermophilic archaeon Sulfolobus acidocaldarius. J Mol Evol. 1997;45:107-14 pubmed
    We have isolated a new gene encoding a putative 103-kDa protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Analysis of the deduced amino-acid sequence shows an extended central domain, predicted to form coiled-coil ..
  51. Klenk H, Schleper C, Schwass V, Brudler R. Nucleotide sequence, transcription and phylogeny of the gene encoding the superoxide dismutase of Sulfolobus acidocaldarius. Biochim Biophys Acta. 1993;1174:95-8 pubmed
    The gene encoding the superoxide dismutase (SOD) of the thermophilic archaeon Sulfolobus acidocaldarius has been isolated and sequenced. Both the start site and the termination sites of the corresponding transcript were mapped...
  52. Charlier D, Roovers M, Thia Toong T, Durbecq V, Glansdorff N. Cloning and identification of the Sulfolobus solfataricus lrp gene encoding an archaeal homologue of the eubacterial leucine-responsive global transcriptional regulator Lrp. Gene. 1997;201:63-8 pubmed
  53. Robinson H, Gao Y, McCrary B, Edmondson S, Shriver J, Wang A. The hyperthermophile chromosomal protein Sac7d sharply kinks DNA. Nature. 1998;392:202-5 pubmed publisher
    ..Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively...
  54. Ohnuma S, Suzuki M, Nishino T. Archaebacterial ether-linked lipid biosynthetic gene. Expression cloning, sequencing, and characterization of geranylgeranyl-diphosphate synthase. J Biol Chem. 1994;269:14792-7 pubmed
    Archaebacterial Sulfolobus acidocaldarius geranylgeranyl-diphosphate (GGPP) synthase (EC 2.5.1...
  55. Ohto C, Nakane H, Hemmi H, Ohnuma S, Obata S, Nishino T. Overexpression of an archaeal geranylgeranyl diphosphate synthase in Escherichia coli cells. Biosci Biotechnol Biochem. 1998;62:1243-6 pubmed
    ..The MBP-fusion protein has protease-sensitive sites in the portion corresponding to geranylgeranyl diphosphate synthase...
  56. Vonrhein C, B nisch H, Sch fer G, Schulz G. The structure of a trimeric archaeal adenylate kinase. J Mol Biol. 1998;282:167-79 pubmed publisher
    The adenylate kinase from the hyperthermophilic archaean species Sulfolobus acidocaldarius has been cloned, expressed in Escherichia coli, purified and crystallized...
  57. Song T, Ding M, Zhai F, Liu D, Liu H, Xiao W, et al. Engineering Saccharomyces cerevisiae for geranylgeraniol overproduction by combinatorial design. Sci Rep. 2017;7:14991 pubmed publisher
    ..11 diverse promoters, a fusion gene BTS1-ERG20, a heterologous geranylgeranyl diphosphate synthase from Sulfolobus acidocaldarius (GGPPSsa) and an endogenous N-terminal truncated gene 3-hydroxyl-3-methylglutaryl-CoA reductase ..
  58. Leppänen V, Nummelin H, Hansen T, Lahti R, Schafer G, Goldman A. Sulfolobus acidocaldarius inorganic pyrophosphatase: structure, thermostability, and effect of metal ion in an archael pyrophosphatase. Protein Sci. 1999;8:1218-31 pubmed
    ..crystal structure of an inorganic pyrophosphatase (S-PPase) from an archaebacterium, the thermophile Sulfolobus acidocaldarius, has been solved by molecular replacement and refined to an R-factor of 19.7% at 2.7 A...
  59. Omer A, Lowe T, Russell A, Ebhardt H, Eddy S, Dennis P. Homologs of small nucleolar RNAs in Archaea. Science. 2000;288:517-22 pubmed
    ..Eighteen small sno-like RNAs (sRNAs) were cloned from the archaeon Sulfolobus acidocaldarius by coimmunoprecipitation with archaeal fibrillarin and NOP56, the homologs of eukaryotic snoRNA-..
  60. Hemmi H, Yamashita S, Shimoyama T, Nakayama T, Nishino T. Cloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius. J Bacteriol. 2001;183:401-4 pubmed
    ..In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.
  61. Cardona S, Remonsellez F, Guiliani N, Jerez C. The glycogen-bound polyphosphate kinase from Sulfolobus acidocaldarius is actually a glycogen synthase. Appl Environ Microbiol. 2001;67:4773-80 pubmed
    ..for a glycogen-bound protein of 57 kDa with PPK as well as glycosyl transferase (GT) activities from Sulfolobus acidocaldarius (R. Skórko, J. Osipiuk, and K. O. Stetter, J. Bacteriol. 171:5162-5164, 1989)...
  62. Constantinesco F, Forterre P, Elie C. NurA, a novel 5'-3' nuclease gene linked to rad50 and mre11 homologs of thermophilic Archaea. EMBO Rep. 2002;3:537-42 pubmed publisher
    ..exonuclease activity on single-stranded and double-stranded DNA from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Nuclease homologs are detected in all thermophilic archaea and, in most species, the nurA gene is ..
  63. Wilson R, Pata J. Structural insights into the generation of single-base deletions by the Y family DNA polymerase dbh. Mol Cell. 2008;29:767-79 pubmed publisher
  64. Langer D, Hain J, Thuriaux P, Zillig W. Transcription in archaea: similarity to that in eucarya. Proc Natl Acad Sci U S A. 1995;92:5768-72 pubmed
    ..The sequences of the Sulfolobus acidocaldarius subunits D, E, and N and alignments with eucaryal homologs are presented here...