Thermococcus onnurineus NA1


Alias: Thermococcus onnurineus NA-1, Thermococcus onnurineus str. NA1, Thermococcus onnurineus strain NA1

Top Publications

  1. Lee H, Kim Y, Bae S, Jeon J, Lim J, Kang S, et al. Overexpression and characterization of a carboxypeptidase from the hyperthermophilic archaeon Thermococcus sp. NA1. Biosci Biotechnol Biochem. 2006;70:1140-7 pubmed
    ..This broad specificity was confirmed by C-terminal ladder sequencing of porcine N-acetyl-renin substrate by TNA1_CP. ..
  2. Lee H, Kim Y, Bae S, Jeon J, Lim J, Jeong B, et al. Cloning, expression, and characterization of a methionyl aminopeptidase from a hyperthermophilic archaeon Thermococcus sp. NA1. Mar Biotechnol (NY). 2006;8:425-32 pubmed
    ..It seemed likely that the cysteine residue (Cys53) played a critical role in regulating the thermostability of TNA1_MetAP. ..
  3. Lee H, Kim Y, Cho Y, Kim S, Lee J, Kang S. Characterization of prolyl oligopeptidase from hyperthermophilic archaeon Thermococcus sp. NA1. J Biosci Bioeng. 2007;103:221-8 pubmed
    ..Autodegradation fragments ranging from 2 to 18 kDa were mapped by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. ..
  4. Lee H, Cho Y, Kim Y, Nam K, Lee J, Kang S. Biochemical characterization of deblocking aminopeptidase from hyperthermophilic archaeon Thermococcus onnurineus NA1. J Biosci Bioeng. 2007;104:188-94 pubmed
    ..The enzyme activated by Co(2+) ions appeared to eventually be inactivated by autodegradation, which was confirmed by mass spectrometry. ..
  5. Lee H, Cho Y, Kim Y, Lho T, Cha S, Lee J, et al. A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1. FEMS Microbiol Lett. 2009;300:68-74 pubmed publisher
    The TON_0002 gene, which is in close proximity to the DNA polymerase locus in Thermococcus onnurineus NA1, has been shown to encode an inorganic pyrophosphatase...
  6. An Y, Lee C, Supangat S, Lee H, Lee J, Kang S, et al. Crystallization and preliminary X-ray crystallographic analysis of Lon from Thermococcus onnurineus NA1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:54-6 pubmed publisher
    ..Lon from Thermococcus onnurineus NA1 was purified and crystallized at 295 K. A 2...
  7. Lee Y, Leem S, Chung Y, Kim S. Characterization of thermostable deblocking aminopeptidases of archaeon Thermococcus onnurineus NA1 by proteomic and biochemical approaches. J Microbiol. 2012;50:792-7 pubmed publisher
    b>Thermococcus onnurineus NA1 is a hyperthermophilic archaeon that grows optimally at >80°C. The deblocking aminopeptidase (DAP) (TNA1-DAP1) encoded in Ton_1032 of T. onnurineus NA1 is considered a major DAP...
  8. Jung T, Kim Y, Oh B, Woo E. Identification of a novel ligand binding site in phosphoserine phosphatase from the hyperthermophilic archaeon Thermococcus onnurineus. Proteins. 2013;81:819-29 pubmed publisher
    ..onnurineus. Identification of this novel binding site distantly located from the catalytic site may be exploited for the development of effective therapeutic allosteric inhibitors against PSP activity. ..
  9. Kim M, An Y, Cha S. The crystal structure of a novel phosphopantothenate synthetase from the hyperthermophilic archaea, Thermococcus onnurineus NA1. Biochem Biophys Res Commun. 2013;439:533-8 pubmed publisher
    ..The TON1374 protein from Thermococcus onnurineus NA1 is a PPS, that shares 54% sequence identity with the first reported archaeal PPS candidate, MM2281, ..

More Information


  1. Kim Y, Lee H, Kwon S, Lee J, Kang S. Enhancing the processivity of a family B-type DNA polymerase of Thermococcus onnurineus and application to long PCR. Biotechnol Lett. 2014;36:985-92 pubmed publisher
    ..Here, we have red the exonuclease domain of the Thermococcus onnurineus NA1 (TNA1) DNA polymerase, especially Asn210 to Asp215 residues in Exo II motif (NXXXFD), to improve the ..
  2. Jung T, Park K, An Y, Schulga A, Deyev S, Jung J, et al. Structural features of Cas2 from Thermococcus onnurineus in CRISPR-cas system type IV. Protein Sci. 2016;25:1890-7 pubmed publisher
    ..These structural features could be a unique signature and indicate an altered functional mechanism in the adaptation stage of Cas2 in type IV CRISPR-Cas systems. ..
  3. Sohn Y, Lee S, Lee K, Ku B, Shin H, Cha S, et al. Identification of a Highly Conserved Hypothetical Protein TON_0340 as a Probable Manganese-Dependent Phosphatase. PLoS ONE. 2016;11:e0167549 pubmed publisher
    ..The Mn2+-bound TON_0340 structure provides a canonical model for the ubiquitously present TON_0340 homologues and lays a strong foundation for the elucidation of their substrate and biological function. ..
  4. Cha S, An Y, Lee C, Lee H, Kim Y, Kim S, et al. Crystal structure of Lon protease: molecular architecture of gated entry to a sequestered degradation chamber. EMBO J. 2010;29:3520-30 pubmed publisher
    ..We report the 2.0-Å resolution crystal structure of Thermococcus onnurineus NA1 Lon (TonLon)...
  5. Jeong J, Kim Y, Rojvirija C, Cha H, Kim Y, Ha S. Structure of the hypothetical protein Ton1535 from Thermococcus onnurineus NA1 reveals unique structural properties by a left-handed helical turn in normal ?-solenoid protein. Proteins. 2014;82:1072-8 pubmed publisher
    The crystal structure of Ton1535, a hypothetical protein from Thermococcus onnurineus NA1, was determined at 2.3 Å resolution...