Treponema denticola ATCC 35405


Alias: Treponema denticola str. ATCC 35405

Top Publications

  1. Bamford C, Fenno J, Jenkinson H, Dymock D. The chymotrypsin-like protease complex of Treponema denticola ATCC 35405 mediates fibrinogen adherence and degradation. Infect Immun. 2007;75:4364-72 pubmed
    ..denticola cells, but not strain CKE (CTLP(-)) cells, were extended. These results suggest that interactions of T. denticola with fibrinogen, which may promote colonization and modulate hemostasis, are mediated principally by CTLP...
  2. Fenno J, Müller K, McBride B. Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola. J Bacteriol. 1996;178:2489-97 pubmed
    The gene encoding the major outer sheath protein (Msp) of the oral spirochete Treponema denticola ATCC 35405 was cloned, sequenced, and expressed in Escherichia coli...
  3. Xu X, Holt S, Kolodrubetz D. Cloning and expression of two novel hemin binding protein genes from Treponema denticola. Infect Immun. 2001;69:4465-72 pubmed
    ..coli. The hbpB gene product is 49% identical to HbpA and binds hemin. Thus, T. denticola has two novel hemin binding proteins which may be part of a previously unrecognized iron acquisition pathway. ..
  4. Anand A, Luthra A, Edmond M, Ledoyt M, Caimano M, Radolf J. The major outer sheath protein (Msp) of Treponema denticola has a bipartite domain architecture and exists as periplasmic and outer membrane-spanning conformers. J Bacteriol. 2013;195:2060-71 pubmed publisher
    ..Extrapolated to T. pallidum, our model enables us to explain how individual Tprs can localize to either the periplasmic (e.g., TprK) or OM (e.g., TprC) compartments...
  5. Bian J, Shen H, Tu Y, Yu A, Li C. The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola. J Bacteriol. 2011;193:3912-22 pubmed publisher
  6. Godovikova V, Goetting Minesky M, Shin J, Kapila Y, Rickard A, Fenno J. A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405. Appl Environ Microbiol. 2015;81:6496-504 pubmed publisher
    ..Addition of routine plasmid-based fluorescence labeling to the Treponema toolset will enable more-rigorous and -detailed studies of the behavior of this organism. ..
  7. Lee S, Bian X, Wong G, Hannam P, McBride B, Fenno J. Cleavage of Treponema denticola PrcA polypeptide to yield protease complex-associated proteins Prca1 and Prca2 is dependent on PrtP. J Bacteriol. 2002;184:3864-70 pubmed
    ..These data indicate that PrtP is required for cleavage of PrcA and suggest that this cleavage may be required for formation or stability of outer membrane complexes. ..
  8. Bian J, Li C. Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector. Appl Environ Microbiol. 2011;77:4573-8 pubmed publisher
    ..The study described here provides us with a new tool and strategy to genetically manipulate T. denticola, in particular ATCC 35405, and other strains that may carry similar endonucleases. ..
  9. Heinzerling H, Olivares M, Burne R. Genetic and transcriptional analysis of flgB flagellar operon constituents in the oral spirochete Treponema denticola and their heterologous expression in enteric bacteria. Infect Immun. 1997;65:2041-51 pubmed
    ..reagent to isolate recombinant bacteriophage lambda clones expressing antigens of the oral spirochete Treponema denticola ATCC 35405. Nucleotide sequence analysis of a clone expressing three immunoreactive products has revealed seven T...

More Information


  1. Chu L, Burgum A, Kolodrubetz D, Holt S. The 46-kilodalton-hemolysin gene from Treponema denticola encodes a novel hemolysin homologous to aminotransferases. Infect Immun. 1995;63:4448-55 pubmed
    ..This suggests that the 46-kDa hemolysin may be related to an aminotransferase and have a novel mechanism of hemolysis. However, the functional aspects of this relationship remain to be investigated. ..
  2. Hu K, Zhao M, Zhang T, Zha M, Zhong C, Jiang Y, et al. Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutylicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism. Biochem J. 2013;449:79-89 pubmed publisher
    ..A modelling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs. ..
  3. Arakawa S, Kuramitsu H. Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola. Infect Immun. 1994;62:3424-33 pubmed
    ..The availability of the purified protein and its gene will aid in evaluating the potential role of the protease in the physiology and virulence of T. denticola since proteases may play a key role in oral treponemal pathogenicity. ..
  4. Ishihara K, Ishihara M, Takazoe I, Okuda K. Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola. Appl Environ Microbiol. 1992;58:3399-403 pubmed
    ..The deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of Escherichia coli. The gene products showed aspartate carbamoyltransferase activity. ..
  5. Tranchimand S, Starks C, Mathews I, Hockings S, Kappock T. Treponema denticola PurE Is a bacterial AIR carboxylase. Biochemistry. 2011;50:4623-37 pubmed publisher
    ..In the human pathogen T. denticola, purine biosynthesis should depend on available CO(2) levels. Because spirochetes lack carbonic anhydrase, the corresponding reduction in bicarbonate demand may confer a selective advantage. ..
  6. Ishihara K, Kuramitsu H. Cloning and expression of a neutral phosphatase gene from Treponema denticola. Infect Immun. 1995;63:1147-52 pubmed
    We have isolated and characterized a neutral phosphatase gene, phoN, from Treponema denticola ATCC 35405. The gene was isolated from a T. denticola clone bank constructed in the medium-copy-number plasmid vector pMCL19...
  7. Xu X, Steffensen B, Robichaud T, Mikhailova M, Lai V, Montgomery R, et al. Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola. Anaerobe. 2015;36:39-48 pubmed publisher
    ..When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells. ..
  8. Kent C, Gee P, Lee S, Bian X, Fenno J. A CDP-choline pathway for phosphatidylcholine biosynthesis in Treponema denticola. Mol Microbiol. 2004;51:471-81 pubmed
    ..Thus it appears that T. denticola does contain a licCA-dependent CDP-choline pathway for phosphatidylcholine biosynthesis. ..
  9. Jones M, Vanyo S, Visser M. The C-terminal region of the major outer sheath protein of Treponema denticola inhibits neutrophil chemotaxis. Mol Oral Microbiol. 2017;32:375-389 pubmed publisher
    ..Knowledge of how spirochetes dampen the neutrophil response is limited and Msp may represent a novel therapeutic target for periodontal disease. ..
  10. Stamm L, Bergen H. Molecular characterization of a flagellar (fla) operon in the oral spirochete Treponema denticola ATCC 35405. FEMS Microbiol Lett. 1999;179:31-6 pubmed
    ..pallidum fla promoter, was identified upstream of tap1. These results showed that the T. denticola and T. pallidum fla operons are highly conserved, supporting the proposed phylogenetic relatedness of these spirochetes. ..
  11. Stamm L, Bergen H. Molecular characterization of the Treponema denticola fliQ region. DNA Seq. 2001;12:463-7 pubmed
    ..The location of the T. denticola fliQ-flhA' genes differs from that of the corresponding T. pallidum and Borrelia burgdorferi genes which are present in the large fla or flgB flagellar operons, respectively. ..
  12. Heinzerling H, Penders J, Burne R. Identification of a fliG homologue in Treponema denticola. Gene. 1995;161:69-73 pubmed
    ..The stronger homology of the spirochete FliG with those of Gram+ bacteria is also consistent with recent analyses of other spirochetal genes. ..
  13. Bond Watts B, Weeks A, Chang M. Biochemical and structural characterization of the trans-enoyl-CoA reductase from Treponema denticola. Biochemistry. 2012;51:6827-37 pubmed
  14. Greene S, Stamm L. Molecular characterization of the gyrB region of the oral spirochete, Treponema denticola. Gene. 2000;253:259-69 pubmed
    ..The mutants have a >20-fold higher resistance to coumermycin A(1) than wild-type Td. A single point mutation in gyrB that changed GyrB Lys(136) to Glu or Thr appears to be responsible for the coumermycin A(1) resistance. ..
  15. Slivienski Gebhardt L, Izard J, Samsonoff W, Limberger R. Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola. Infect Immun. 2004;72:5493-7 pubmed
    ..This shuttle plasmid enables high-level expression of genes in T. denticola and possesses an efficient selectable marker that provides a new tool for treponemal genetics. ..
  16. Bian X, Wang H, Ning Y, Lee S, Fenno J. Mutagenesis of a novel gene in the prcA-prtP protease locus affects expression of Treponema denticola membrane complexes. Infect Immun. 2005;73:1252-5 pubmed publisher
    ..Together, these results provide information on the assembly of outer membrane complexes involved in T. denticola interaction with host cells and tissue...
  17. Fenno J, Tamura M, Hannam P, Wong G, Chan R, McBride B. Identification of a Treponema denticola OppA homologue that binds host proteins present in the subgingival environment. Infect Immun. 2000;68:1884-92 pubmed
    ..Binding of soluble host proteins by OppA may be important both for spirochete-host interactions in the subgingival environment and for uptake of peptide nutrients...
  18. Fenno J, Wong G, Hannam P, M ller K, Leung W, McBride B. Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp. J Bacteriol. 1997;179:1082-9 pubmed
    ..Further studies of the antigenic domains, functional domains, and physical structures of Msp proteins, based on these results, will enhance understanding of the role of Msp in the cytopathology associated with oral spirochetes...
  19. Li H, Arakawa S, Deng Q, Kuramitsu H. Characterization of a novel methyl-accepting chemotaxis gene, dmcB, from the oral spirochete Treponema denticola. Infect Immun. 1999;67:694-9 pubmed
    Immediately downstream from the previously isolated Treponema denticola ATCC 35405 prtB gene coding for a chymotrypsinlike protease activity, an open reading frame, ORF3, was identified which shared significant homology with the highly ..
  20. Abiko Y, Nagano K, Yoshida Y, Yoshimura F. Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. PLoS ONE. 2014;9:e89051 pubmed publisher
    ..In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear...
  21. Kurniyati K, Zhang W, Zhang K, Li C. A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola. Mol Microbiol. 2013;89:842-56 pubmed publisher
    ..Based on these results, we propose that T.denticola has evolved a strategy to scavenge host sialic acid using its neuraminidase, which allows the spirochaete to acquire nutrients and evade complement killing...
  22. Bian J, Liu X, Cheng Y, Li C. Inactivation of cyclic Di-GMP binding protein TDE0214 affects the motility, biofilm formation, and virulence of Treponema denticola. J Bacteriol. 2013;195:3897-905 pubmed publisher
    ..This report also paves a way to further unveil the roles of the c-di-GMP signaling network in the biology and pathogenicity of T. denticola...
  23. Greene S, Stamm L. Molecular characterization of a chemotaxis operon in the oral spirochete, Treponema denticola. Gene. 1999;232:59-68 pubmed
    ..denticola CheA and CheY proteins are highly conserved with those of the Escherichia coli (Ec) CheA and CheY proteins. Phylogenetic analysis of Td CheY indicated that it is closely related to Tp CheY and Bb CheY3...
  24. Limberger R, Slivienski L, Izard J, Samsonoff W. Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks. J Bacteriol. 1999;181:3743-50 pubmed
    ..We propose that treponemal Tap1 is analogous to FliK, which is involved in monitoring the flagellar hook length of Salmonella typhimurium...
  25. Fenno J, Lee S, Bayer C, Ning Y. The opdB locus encodes the trypsin-like peptidase activity of Treponema denticola. Infect Immun. 2001;69:6193-200 pubmed publisher
    ..The mutant lacked BANA-hydrolyzing activity and had a slightly slower growth rate than the parent strain. This mutant will be used in future studies of interactions of T. denticola with host cells and tissue...
  26. Krupka H, Huber R, Holt S, Clausen T. Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme. EMBO J. 2000;19:3168-78 pubmed publisher
    ..Cystalysin represents an essential factor of adult periodontitis, therefore the structure of the cystalysin-AVG complex may provide the chemical basis for rational drug design...
  27. Godovikova V, Wang H, Goetting Minesky M, Ning Y, Capone R, Slater C, et al. Treponema denticola PrcB is required for expression and activity of the PrcA-PrtP (dentilisin) complex. J Bacteriol. 2010;192:3337-44 pubmed publisher
    ..Taken together, these data are consistent with identification of PrcB as a PrtP-binding lipoprotein that likely stabilizes the PrtP polypeptide during localization to the outer membrane...
  28. Brett P, Burtnick M, Fenno J, Gherardini F. Treponema denticola TroR is a manganese- and iron-dependent transcriptional repressor. Mol Microbiol. 2008;70:396-409 pubmed publisher
    ..Based upon these findings, we propose that TroR(Td) represents a novel member of the DtxR family of transcriptional regulators and is likely to play an important role in regulating both manganese and iron homeostases in this spirochaete...
  29. Frederick J, Rogers E, Marconi R. Analysis of a growth-phase-regulated two-component regulatory system in the periodontal pathogen Treponema denticola. J Bacteriol. 2008;190:6162-9 pubmed publisher
    ..These analyses represent the first description of a functional TCS in an oral spirochetes and provide insight into the transcriptional regulatory mechanisms of these important bacteria...
  30. Ikegami A, Honma K, Sharma A, Kuramitsu H. Multiple functions of the leucine-rich repeat protein LrrA of Treponema denticola. Infect Immun. 2004;72:4619-27 pubmed publisher
    ..LrrA, that contains eight consensus tandem repeats of 23 amino acid residues, has been identified in Treponema denticola ATCC 35405. A leucine-rich repeat is a generally useful protein-binding motif, and proteins containing this ..
  31. Tucci S, Martin W. A novel prokaryotic trans-2-enoyl-CoA reductase from the spirochete Treponema denticola. FEBS Lett. 2007;581:1561-6 pubmed publisher
  32. McDowell J, Frederick J, Stamm L, Marconi R. Identification of the gene encoding the FhbB protein of Treponema denticola, a highly unique factor H-like protein 1 binding protein. Infect Immun. 2007;75:1050-4 pubmed publisher
    ..The identification of FhbB represents an important step in understanding the contribution of FHL-1 binding in T. denticola pathogenesis and in development of periodontal disease...
  33. Chen M, Han G, Dietrich C, Dunn T, Cahoon E. The essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the Arabidopsis LCB1 subunit of serine palmitoyltransferase. Plant Cell. 2006;18:3576-93 pubmed publisher
    ..These results demonstrate that plant SPT is a heteromeric enzyme and that sphingolipids are essential components of plant cells and contribute to growth and development...
  34. Lepine G, Ellen R. MglA and mglB of Treponema denticola; similarity to ABC transport and spa genes. DNA Seq. 2000;11:419-31 pubmed
    ..denticola as well as in E. coli cells as determined by reverse transcription PCR (RT-PCR). Homology to td-mglB and its expressed protein was found in other oral spirochetes as determined by Southern and western blot analysis...
  35. Figge R, Cerff R. GAPDH gene diversity in spirochetes: a paradigm for genetic promiscuity. Mol Biol Evol. 2001;18:2240-9 pubmed publisher
    ..Furthermore, we provide evidence that the GAPDH genes present in the protists Parabasalia may also be of spirochetal descent...