Leptospira interrogans serovar Lai str. 56601



Top Publications

  1. Ballard S, Go M, Segers R, Adler B. Molecular analysis of the dnaK locus of Leptospira interrogans serovar Copenhageni. Gene. 1998;216:21-9 pubmed
    ..These results indicate that the regulation of the dnaK locus of Leptospira differs somewhat from that observed in other organisms. ..
  2. Picardeau M, Brenot A, Saint Girons I. First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella. Mol Microbiol. 2001;40:189-99 pubmed
    ..Our results demonstrate that FlaB is involved in flagella assembly and motility. They also show the feasibility of performing allelic replacement in Leptospira spp. by homologous recombination...
  3. Buyuktimkin B, Saier M. Comparative genomic analyses of transport proteins encoded within the genomes of Leptospira species. Microb Pathog. 2015;88:52-64 pubmed publisher
    ..The variability of host tropism and mortality rates by infectious leptospires suggests that small differences in individual sets of proteins play important physiological and pathological roles. ..
  4. Buyuktimkin B, Saier M. Comparative analyses of transport proteins encoded within the genomes of Leptospira species. Microb Pathog. 2016;98:118-31 pubmed publisher
    ..The variability of host tropism and mortality rates by infectious leptospires suggests that small differences in individual sets of proteins play important physiological and pathological roles. ..
  5. Haake D, Champion C, Martinich C, Shang E, Blanco D, Miller J, et al. Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp. J Bacteriol. 1993;175:4225-34 pubmed
    ..OmpL1 may be the first spirochetal transmembrane outer membrane protein for which the structural gene has been cloned and sequenced...
  6. Koizumi N, Watanabe H. Identification of a novel antigen of pathogenic Leptospira spp. that reacted with convalescent mice sera. J Med Microbiol. 2003;52:585-9 pubmed publisher
    ..The gene for LAg42 is conserved among pathogenic leptospires but not among non-pathogenic leptospires, which suggests its involvement in virulence...
  7. Artiushin S, Timoney J, Nally J, Verma A. Host-inducible immunogenic sphingomyelinase-like protein, Lk73.5, of Leptospira interrogans. Infect Immun. 2004;72:742-9 pubmed
    ..The host-inducible immunogenic Lk73.5 should have value in distinguishing vaccine from infection immune response...
  8. Brenot A, Werts C, Ottone C, Sertour N, Charon N, Postic D, et al. First evidence for a restriction-modification system in Leptospira sp. FEMS Microbiol Lett. 2001;201:139-43 pubmed
    ..biflexa. The evolutionary significance and the importance of the restriction and modification enzymes or non-specific nucleases within strains for genetic experiments are discussed. ..
  9. Ma J, Zhang P, Zhang Z, Zha M, Xu H, Zhao G, et al. Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans. Biochem J. 2008;415:45-56 pubmed publisher

More Information


  1. Zhou Z, Song X, Gong W. Novel conformational states of peptide deformylase from pathogenic bacterium Leptospira interrogans: implications for population shift. J Biol Chem. 2005;280:42391-6 pubmed publisher
    ..These results not only facilitate the development of peptide deformylase-targeted drugs but also provide structural insights into the mechanism of an unusual type of protein binding event...
  2. Haake D, Matsunaga J. Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins. Infect Immun. 2002;70:4936-45 pubmed
    ..The 48-kDa OMP was identified as a novel OM lipoprotein designated LipL48. The use of membrane-specific markers in OM isolation techniques facilitates an accurate description of the leptospiral OM and its components...
  3. Cullen P, Haake D, Bulach D, Zuerner R, Adler B. LipL21 is a novel surface-exposed lipoprotein of pathogenic Leptospira species. Infect Immun. 2003;71:2414-21 pubmed
    ..These findings show that LipL21 is a surface-exposed, abundant outer membrane lipoprotein that is expressed during infection and conserved among pathogenic Leptospira species...
  4. Baril C, Richaud C, Fournie E, Baranton G, Saint Girons I. Cloning of dapD, aroD and asd of Leptospira interrogans serovar icterohaemorrhagiae, and nucleotide sequence of the asd gene. J Gen Microbiol. 1992;138:47-53 pubmed
    ..coli enzymes was less than 31%. Highly conserved sequences within aspartate semialdehyde dehydrogenase from the five organisms were observed at the amino and carboxyl termini, and around the cysteine of the active site. ..
  5. Palaniappan R, Chang Y, Hassan F, McDonough S, Pough M, Barr S, et al. Expression of leptospiral immunoglobulin-like protein by Leptospira interrogans and evaluation of its diagnostic potential in a kinetic ELISA. J Med Microbiol. 2004;53:975-84 pubmed publisher
    ..These findings suggest that recombinant antigen to the conserved region of LigA and LigB can differentiate between vaccinated and naturally infected animals...
  6. Choudhary M, Jawaid S, Ahuja M, Shiva N, Gupta P, Bhuyan A, et al. Open reading frame yjbI of Bacillus subtilis codes for truncated hemoglobin. Protein Expr Purif. 2005;41:363-72 pubmed
    ..Presumably, only a small portion of the protein is involved in peroxidase activity, which is exposed with increasing concentration of the denaturants. ..
  7. Asuthkar S, Velineni S, Stadlmann J, Altmann F, Sritharan M. Expression and characterization of an iron-regulated hemin-binding protein, HbpA, from Leptospira interrogans serovar Lai. Infect Immun. 2007;75:4582-91 pubmed publisher
    ..This protein was seen in several serovars, including nonpathogenic L. biflexa. Sequence analysis and immunoreactivity with specific antibodies showed this protein to be LipL41...
  8. Wang M, Liu L, Wang Y, Wei Z, Zhang P, Li Y, et al. Crystal structure of homoserine O-acetyltransferase from Leptospira interrogans. Biochem Biophys Res Commun. 2007;363:1050-6 pubmed
    ..Detailed structure analysis showed that the catalytic histidine and serine are both present in two conformations, which may be involved in the catalytic mechanism for acetyl transfer. ..
  9. Ruan P, Wang X, Sun A, Li S, Yan J. [Association of fliR gene in Leptospira interrogans with adhesion and pathogenicity to host cells]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2008;37:572-8 pubmed
    ..01). fliR is a virulence-associated gene of L. interrogans and the function of the gene is closely related to adhesion, induction of cell necrosis and apoptosis of the microbe. ..
  10. Sun Z, Bao L, Li D, Huang B, Wu B. Effect of Leptospira interrogans outer membrane proteins LipL32 on HUVEC. Microb Pathog. 2010;49:116-21 pubmed publisher
    ..Moreover, apoptosis of HUVEC was significantly accelerated. But the fusion partner had no effect in these regards. It is possible that LipL32 is involved in the vessel lesions. ..
  11. Kucerová P, Cermakova Z, Pliskova L, Pavlis O, Kubickova P, Kleprlikova H, et al. Our experience using real-time PCR for the detection of the gene that encodes the superficial lipoprotein LipL32 of the pathogenic leptospires to confirm the acute form of human leptospirosis. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013;157:387-91 pubmed publisher
    ..As demonstrated in the study, the real-time PCR method for detecting the gene for the superficial lipoprotein LipL32 is an appropriate, quick and reliable method for the diagnosis of the acute form of leptospirosis...
  12. King A, Bartpho T, Sermswan R, Bulach D, Eshghi A, Picardeau M, et al. Leptospiral outer membrane protein LipL41 is not essential for acute leptospirosis but requires a small chaperone protein, lep, for stable expression. Infect Immun. 2013;81:2768-76 pubmed publisher
    ..The roles of LipL41 and Lep in the pathogenesis of Leptospira interrogans were investigated; surprisingly, neither of these two unique proteins was essential for acute leptospirosis...
  13. Schons Fonseca L, da Silva J, Milanez J, Domingos R, Smith J, Nakaya H, et al. Analysis of LexA binding sites and transcriptomics in response to genotoxic stress in Leptospira interrogans. Nucleic Acids Res. 2016;44:1179-91 pubmed publisher
    ..Our findings indicate that there is a shift in gene expression following DNA damage that represses genes involved in cell growth and virulence, and induces genes involved in mutagenesis and recombination. ..
  14. Anita K, Premlatha M, Kanagavel M, Akino Mercy C, Raja V, Shanmughapriya S, et al. Evaluation of combined B cell specific N-terminal immunogenic domains of LipL21 for diagnosis of leptospirosis. Int J Biol Macromol. 2016;91:465-70 pubmed publisher
    ..The I-LipL21 dot blot assay showed a further increased sensitivity of 3.8% over the IgM-ELISA. Therefore I-LipL21 may be the ideal candidate protein for diagnosis of leptospirosis. ..
  15. Denipitiya D, Chandrasekharan N, Abeyewickreme W, Hartskeerl R, Hapugoda M. Identification of cattle, buffaloes and rodents as reservoir animals of Leptospira in the District of Gampaha, Sri Lanka. BMC Res Notes. 2017;10:134 pubmed publisher
    ..Therefore, there is a potential zoonotic risk to public health, most notably to farmers in this area. ..
  16. Zuerner R, Charon N. Nucleotide sequence analysis of the Leptospira biflexa serovar patoc rpsL and rpsG genes. J Bacteriol. 1990;172:6165-8 pubmed
    ..biflexa genes had several unusual features when compared with homologous proteins of other organisms. Unlike the rpsL genes of other eubacteria, the L. biflexa rpsL gene is adjacent to a rpoC-like gene. ..
  17. Takahashi Y, Akase K, Hirano H, Fukunaga M. Physical and genetic maps of the Leptospira interrogans serovar icterohaemorrhagiae strain Ictero no.1 chromosome and sequencing of a 19-kb region of the genome containing the 5S rRNA gene. Gene. 1998;215:37-45 pubmed
    ..The rest of the putative products encoded by ORFs in the sequenced region showed little similarity with the proteins contained in the databases and were considered to be unknown proteins. ..
  18. Li Y, Ren S, Gong W. Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans. Acta Crystallogr D Biol Crystallogr. 2002;58:846-8 pubmed
    ..interrogans was purified from 1 l of cell culture. Crystallization screening of the purified enzyme resulted in two crystal forms, from one of which a 3 A resolution X-ray diffraction data set has been collected...
  19. Lee S, Kim K, Park Y, Seong I, Kim M, Lee Y. Identification and partial characterization of a novel hemolysin from Leptospira interrogans serovar lai. Gene. 2000;254:19-28 pubmed
    ..biflexa serovar patoc. We concluded that the SphH is a novel hemolysin of a pathogenic Leptospira species, which may be a putative pore-forming protein...
  20. Mitchison M, Bulach D, Vinh T, Rajakumar K, Faine S, Adler B. Identification and characterization of the dTDP-rhamnose biosynthesis and transfer genes of the lipopolysaccharide-related rfb locus in Leptospira interrogans serovar Copenhageni. J Bacteriol. 1997;179:1262-7 pubmed
    ..Moreover, it could restore normal LPS phenotype to a defined rfbB::Tn5 mutant of S. flexneri which was deficient in all four genes, thereby confirming the functional identification of a part of the leptospiral rfb locus...
  21. Bourhy P, Saint Girons I. Localization of the Leptospira interrogans metF gene on the CII secondary chromosome. FEMS Microbiol Lett. 2000;191:259-63 pubmed
    ..interrogans MetF was found to be thermolabile. The metF gene belonged to the CII secondary chromosome, in contrast to the previously isolated metY and metX genes, which have been localized to the CI chromosome of Leptospira sp. ..
  22. Picardeau M, Ren S, Saint Girons I. Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli. J Bacteriol. 2001;183:6494-7 pubmed publisher
    ..The coexpression of ChpI neutralizes ChpK toxicity. By Southern blot analysis, chp homologs were found in all representative pathogenic strains of L. interrogans...
  23. Figge R, Cerff R. GAPDH gene diversity in spirochetes: a paradigm for genetic promiscuity. Mol Biol Evol. 2001;18:2240-9 pubmed publisher
    ..Furthermore, we provide evidence that the GAPDH genes present in the protists Parabasalia may also be of spirochetal descent...
  24. Shang E, Summers T, Haake D. Molecular cloning and sequence analysis of the gene encoding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species. Infect Immun. 1996;64:2322-30 pubmed
    ..However, in contrast to LipL36, only LipL41 and OmpL1 were exposed on the surface of intact organisms. Immunoblot analysis of a panel of Leptospira species reveals that LipL41 expression is highly conserved among leptospiral pathogens...
  25. Kumar V, Damodharan S, Pandaranayaka E, Madathiparambil M, Tennyson J. Molecular modeling and in-silico engineering of Cardamom mosaic virus coat protein for the presentation of immunogenic epitopes of Leptospira LipL32. J Biomol Struct Dyn. 2016;34:42-56 pubmed publisher
    ..Based on the type of amino acids, length, surface accessibility, and docking energy with CdMV CP model, the order of antigenicity of the LipL32 epitopes was found to be EP4 > EP3 > EP2 > EP6. ..
  26. Gravekamp C, van de Kemp H, Franzen M, Carrington D, Schoone G, van Eys G, et al. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J Gen Microbiol. 1993;139:1691-700 pubmed publisher
  27. Lin M, Surujballi O, Nielsen K, Nadin Davis S, Randall G. Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis. Infect Immun. 1997;65:4355-9 pubmed
  28. Ding M, Yelton D. Cloning and analysis of the leuB gene of Leptospira interrogans serovar pomona. J Gen Microbiol. 1993;139:1093-103 pubmed
    ..8 kDa L. interrogans leuB gene product. The organization of the leucine biosynthetic genes in L. interrogans differs from that found in E. coli, Salmonella typhimurium and Bacillus subtilis. ..
  29. Zhang L, Zhang C, Ojcius D, Sun D, Zhao J, Lin X, et al. The mammalian cell entry (Mce) protein of pathogenic Leptospira species is responsible for RGD motif-dependent infection of cells and animals. Mol Microbiol. 2012;83:1006-23 pubmed publisher
    ..Blockade of the two integrins or the Mce protein decreased leptospiral adherence and invasiveness. The results showed that Mce is an RGD-motif-dependent virulence factor in pathogenic Leptospira species...
  30. Boursaux Eude C, Saint Girons I, Zuerner R. IS1500, an IS3-like element from Leptospira interrogans. Microbiology. 1995;141 ( Pt 9):2165-73 pubmed
    ..interrogans serovars icterohaemorrhagiae and pomona showed insertions were only on the large chromosomal replicon. The location of some IS1500 insertions coincides with regions of the genome that have undergone large rearrangements. ..
  31. Kalambaheti T, Bulach D, Rajakumar K, Adler B. Genetic organization of the lipopolysaccharide O-antigen biosynthetic locus of Leptospira borgpetersenii serovar Hardjobovis. Microb Pathog. 1999;27:105-17 pubmed publisher
    ..Additional ORFs in the locus encode glycosyltransferases for the assembly of the O-antigen subunit and integral membrane proteins for the transport of O-antigen subunits through the membrane and assembly into LPS...
  32. Liao S, Sun A, Ojcius D, Wu S, Zhao J, Yan J. Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogans strain Lai. BMC Microbiol. 2009;9:253 pubmed publisher
    ..The phentotypes observed, including lower pathogenicity, could be a consequence of fliY inactivation, but also a consequence of the polar effects...
  33. Zhao W, Chen C, Zhang X, Lai W, Hu B, Zhao G, et al. Molecular characterization of the pL40 protein in Leptospira interrogans. Can J Microbiol. 2009;55:739-49 pubmed publisher
    ..interrogans. These findings indicate that pL40 may serve as a useful serodiagnostic antigen and vaccine candidate for L. interrogans. ..
  34. Yang J, Zhang Y, Xu J, Geng Y, Chen X, Yang H, et al. Serum activity of platelet-activating factor acetylhydrolase is a potential clinical marker for leptospirosis pulmonary hemorrhage. PLoS ONE. 2009;4:e4181 pubmed publisher
  35. de la Pe a Moctezuma A, Bulach D, Kalambaheti T, Adler B. Comparative analysis of the LPS biosynthetic loci of the genetic subtypes of serovar Hardjo: Leptospira interrogans subtype Hardjoprajitno and Leptospira borgpetersenii subtype Hardjobovis. FEMS Microbiol Lett. 1999;177:319-26 pubmed
  36. Kawabata H, Sakakibara S, Imai Y, Masuzawa T, Fujita H, Tsurumi M, et al. First record of Leptospira borgpetersenii isolation in the Amami Islands, Japan. Microbiol Immunol. 2006;50:429-34 pubmed
    ..The present results suggest that L. borgpetersenii is migrating into the Amami Islands in Japan...
  37. Xue Y, Wei Z, Li X, Gong W. The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis. J Struct Biol. 2006;153:307-11 pubmed publisher
    ..0 and 2.3A resolution, respectively. The structures are similar to the precorrin-8x methyl mutase (CobH), an enzyme of the aerobic pathway to vitamin B12...
  38. de la Pe a Moctezuma A, Bulach D, Adler B. Genetic differences among the LPS biosynthetic loci of serovars of Leptospira interrogans and Leptospira borgpetersenii. FEMS Immunol Med Microbiol. 2001;31:73-81 pubmed
  39. Kawabata H, Dancel L, Villanueva S, Yanagihara Y, Koizumi N, Watanabe H. flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp. Microbiol Immunol. 2001;45:491-6 pubmed
    ..These results suggested that PCR-RFLP based on the flaB gene was an efficient tool for rapid detection and identification of species of infected Leptospira from clinical specimens...
  40. Zhou Z, Song X, Li Y, Gong W. Unique structural characteristics of peptide deformylase from pathogenic bacterium Leptospira interrogans. J Mol Biol. 2004;339:207-15 pubmed publisher
    ..LiPDF displays significant structural differences in the C-terminal region compared to both type-I and type-II PDFs, suggesting a new family of PDFs...
  41. Branger C, Sonrier C, Chatrenet B, Klonjkowski B, Ruvoen Clouet N, Aubert A, et al. Identification of the hemolysis-associated protein 1 as a cross-protective immunogen of Leptospira interrogans by adenovirus-mediated vaccination. Infect Immun. 2001;69:6831-8 pubmed publisher
    ..These data indicate that Hap1 could be a good candidate for developing a new generation of vaccines able to induce broad protection against leptospirosis disease...
  42. Zuerner R, Huang W. Analysis of a Leptospira interrogans locus containing DNA replication genes and a new IS, IS1502. FEMS Microbiol Lett. 2002;215:175-82 pubmed
    ..interrogans serovars by the presence of IS1502. The distribution of IS1502 throughout pathogenic Leptospira species varies. This result suggests that IS1502 may have been recently introduced into Leptospira. ..
  43. Matsunaga J, Young T, Barnett J, Barnett D, Bolin C, Haake D. Novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth-phase-regulated peripheral membrane protein. Infect Immun. 2002;70:323-34 pubmed
    ..kirschneri that colonized the renal tubule. These observations suggest that expression of LipL45 responds to environmental cues, including those encountered during infection of a mammalian host...