Methanosarcina mazei Go1


Alias: Methanosarcina mazei Goe1, Methanosarcina mazei str. Goe1, Methanosarcina mazei str. Go1, Methanosarcina mazei strain Go1

Top Publications

  1. Ehlers C, Grabbe R, Veit K, Schmitz R. Characterization of GlnK1 from Methanosarcina mazei strain Gö1: complementation of an Escherichia coli glnK mutant strain by GlnK1. J Bacteriol. 2002;184:1028-40 pubmed
    ..Because M. mazei GlnK1 was able to complement growth of an E. coli glnK mutant with arginine as the sole nitrogen source, it is likely that uridylylation is not required for its regulatory function. ..
  2. Weidenbach K, Ehlers C, Kock J, Ehrenreich A, Schmitz R. Insights into the NrpR regulon in Methanosarcina mazei Gö1. Arch Microbiol. 2008;190:319-32 pubmed publisher
    ..Deletion of various bases in the promoter region of nifH confirmed that the regulatory element ACC-N(7)-GGT is required for specific binding of NrpRI to the promoter. ..
  3. Ehlers C, Weidenbach K, Veit K, Forchhammer K, Schmitz R. Unique mechanistic features of post-translational regulation of glutamine synthetase activity in Methanosarcina mazei strain Gö1 in response to nitrogen availability. Mol Microbiol. 2005;55:1841-54 pubmed
    ..The GlnK(1) protein of the methanogenic archaeon Methanosarcina mazei strain Go1 interacts and forms stable complexes with glutamine synthetase (GlnA(1))...
  4. Weidenbach K, Ehlers C, Kock J, Schmitz R. NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon Methanosarcina mazei Gö1. FEBS J. 2010;277:4398-411 pubmed publisher
  5. Laine B, Chartier F, Imbert M, Lewis R, Sautiere P. Primary structure of the chromosomal protein HMb from the archaebacteria Methanosarcina barkeri. Eur J Biochem. 1986;161:681-7 pubmed
    ..8% of basic residues) which are distributed all along the polypeptide chain. The amino acid sequence of the protein HMb is not homologous to any eubacterial, archaebacterial or eukaryotic chromosomal proteins known up to now. ..
  6. Maupin Furlow J, Ferry J. A proteasome from the methanogenic archaeon Methanosarcina thermophila. J Biol Chem. 1995;270:28617-22 pubmed
    ..The results suggest that proteasomes are more widespread in the Archaea than previously proposed. Southern blotting experiments suggested the presence of ubiquitin-like sequences in M. thermophila. ..
  7. Buan N, Rehfeld K, Escalante Semerena J. Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1. J Bacteriol. 2006;188:3543-50 pubmed
    ..The data suggested substantial variations in ATP binding and probably corrinoid binding between CobA(Se) and CobA(Mm) enzymes. ..
  8. Macario A, Dugan C, Clarens M, Conway De Macario E. dnaJ in Archaea. Nucleic Acids Res. 1993;21:2773 pubmed
  9. Wilms R, Freiberg C, Wegerle E, Meier I, Mayer F, Muller V. Subunit structure and organization of the genes of the A1A0 ATPase from the Archaeon Methanosarcina mazei Gö1. J Biol Chem. 1996;271:18843-52 pubmed
    ..In summary, it is proposed that the A1A0 ATPase of Methanosarcina mazei Gö1 contains at least nine subunits, of which seven are located in A1 and/or the stalk and two in A0. ..

More Information


  1. Gayen S, Vivekanandan S, Biukovic G, Gruber G, Yoon H. NMR solution structure of subunit F of the methanogenic A1AO adenosine triphosphate synthase and its interaction with the nucleotide-binding subunit B. Biochemistry. 2007;46:11684-94 pubmed
    ..Finally, the arrangement of subunit F within the complex is presented. ..
  2. Deppenmeier U. Different structure and expression of the operons encoding the membrane-bound hydrogenases from Methanosarcina mazei Gö1. Arch Microbiol. 1995;164:370-6 pubmed
    ..In the vho operon, a vhtD-like gene and a terminator composed of tandem repeats could not be identified. The physiological function of two genetically distinct, membrane-bound hydrogenases from Ms. mazei Gö1 is discussed. ..
  3. Yao R, Macario A, Conway De Macario E. An archaeal S-layer gene homolog with repetitive subunits. Biochim Biophys Acta. 1994;1219:697-700 pubmed
  4. Spanheimer R, Hoffmann M, K gl S, Schmidt S, Pfl ger K, M ller V. Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon methanosarcina mazei Gö1. J Mol Microbiol Biotechnol. 2008;15:255-63 pubmed publisher
    ..In addition to osmolarity Ota expression was regulated by the growth phase. Expression of Ota as well as transport of betaine was downregulated in the presence of glycine betaine...
  5. Jeon H, Meng W, Takagi J, Eck M, Springer T, Blacklow S. Implications for familial hypercholesterolemia from the structure of the LDL receptor YWTD-EGF domain pair. Nat Struct Biol. 2001;8:499-504 pubmed publisher
    ..A second subset of FH mutations are located at the interface between the propeller and the C-terminal EGF module, suggesting a structural requirement for maintaining the integrity of the interdomain interface...
  6. Gencic S, Grahame D. Nickel in subunit beta of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. Catalytic properties and evidence for a binuclear Ni-Ni site. J Biol Chem. 2003;278:6101-10 pubmed
    ..A mechanism for C-C bond activation is proposed that includes a specific role for the Fe(4)S(4) center and accounts for the absolute requirement for nickel...
  7. Mayerhofer L, Conway De Macario E, Yao R, Macario A. Structure, organization, and expression of genes coding for envelope components in the archaeon Methanosarcina mazei S-6. Arch Microbiol. 1998;169:339-45 pubmed
  8. Macario A, Dugan C, Conway De Macario E. A dnaK homolog in the archaebacterium Methanosarcina mazei S6. Gene. 1991;108:133-7 pubmed
    ..This finding confirms that DnaK proteins are highly conserved, occurring not only in eubacteria (Bacteria) and eukaryotes (Eucaria), but also in archaebacteria (Archaea)...
  9. Ferguson D, Gorlatova N, Grahame D, Krzycki J. Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 2000;275:29053-60 pubmed publisher
    ..These results indicate MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine...
  10. Sch fer I, Bailer S, D ser M, B rsch M, Bernal R, Stock D, et al. Crystal structure of the archaeal A1Ao ATP synthase subunit B from Methanosarcina mazei Gö1: Implications of nucleotide-binding differences in the major A1Ao subunits A and B. J Mol Biol. 2006;358:725-40 pubmed publisher
    ..The arrangement of subunit B within the intact A1Ao ATP synthase is presented...
  11. Jing H, Takagi J, Liu J, Lindgren S, Zhang R, Joachimiak A, et al. Archaeal surface layer proteins contain beta propeller, PKD, and beta helix domains and are related to metazoan cell surface proteins. Structure. 2002;10:1453-64 pubmed
    ..The third domain is predicted to adopt a beta helix fold. These domains have homologs in metazoan cell surface proteins, suggesting remarkable relationships between domains in archaeal SLPs and metazoan cell surface proteins...
  12. Chien Y, Zinder S. Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum. J Bacteriol. 1994;176:6590-8 pubmed
    ..It is not clear whether this similarity is due to horizontal gene transfer or to the resemblance of the M. barkeri and C. pasteurianum nitrogenase sequences to an ancestral nitrogenase...
  13. Sibold L, Henriquet M, Possot O, Aubert J. Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes. Res Microbiol. 1991;142:5-12 pubmed
    ..These two ORF are followed by nifD in M. ivanovii as well as in the first nifH region of M. barkeri 227. Both types of ORF exhibit a strong homology with the glnB gene...
  14. Thomas T, Cavicchioli R. Archaeal cold-adapted proteins: structural and evolutionary analysis of the elongation factor 2 proteins from psychrophilic, mesophilic and thermophilic methanogens. FEBS Lett. 1998;439:281-6 pubmed
  15. Thomas F, Diaz Bone R, Wuerfel O, Huber B, Weidenbach K, Schmitz R, et al. Connection between multimetal(loid) methylation in methanoarchaea and central intermediates of methanogenesis. Appl Environ Microbiol. 2011;77:8669-75 pubmed publisher
    ..The close connection between metal(loid) methylation and methanogenesis explains the general capability of methanoarchaea to methylate metal(loid)s...
  16. Roessler M, Pfl ger K, Flach H, Lienard T, Gottschalk G, M ller V. Identification of a salt-induced primary transporter for glycine betaine in the methanogen Methanosarcina mazei Gö1. Appl Environ Microbiol. 2002;68:2133-9 pubmed
    ..Northern blot analysis of otaC revealed a salt-dependent transcription of this gene. Ota is the first identified salt-induced transporter for compatible solutes in Archaea...
  17. Tumbula Hansen D, Feng L, Toogood H, Stetter K, S ll D. Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms. J Biol Chem. 2002;277:37184-90 pubmed publisher
    ..The high sequence identity, up to 60% between discriminating and nondiscriminating archaeal AspRSs, suggests that few mutational steps may be necessary to convert the tRNA-discriminating ability of a tRNA synthetase...
  18. SINGH D, Sielaff H, Sundararaman L, Bhushan S, Grüber G. The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase. Biochim Biophys Acta. 2016;1857:177-187 pubmed publisher
  19. Pfl ger K, Baumann S, Gottschalk G, Lin W, Santos H, M ller V. Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity. Appl Environ Microbiol. 2003;69:6047-55 pubmed
    ..These experiments revealed the first genes involved in the biosynthesis of compatible solutes in methanogens...
  20. Woodson J, Escalante Semerena J. CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea. Proc Natl Acad Sci U S A. 2004;101:3591-6 pubmed publisher
    ..Reasons for the evolution of two distinct pathways for Cbi salvaging in prokaryotes are discussed...
  21. Paquet F, Culard F, Barbault F, Maurizot J, Lancelot G. NMR solution structure of the archaebacterial chromosomal protein MC1 reveals a new protein fold. Biochemistry. 2004;43:14971-8 pubmed publisher
    ..Its structure suggests a different DNA-binding mode than those of the histone-like proteins HU or HMGB. Thus, MC1 may be classified as a member of a new family...
  22. Forouhar F, Abashidze M, Xu H, Grochowski L, Seetharaman J, Hussain M, et al. Molecular insights into the biosynthesis of the F420 coenzyme. J Biol Chem. 2008;283:11832-40 pubmed publisher
    ..Large structural differences in the active site region of the non-F(420)-producing CofD homologs suggest that they catalyze a different biochemical reaction...
  23. Ehlers C, J ger D, Schmitz R. Establishing a markerless genetic exchange system for Methanosarcina mazei strain Gö1 for constructing chromosomal mutants of small RNA genes. Archaea. 2011;2011:439608 pubmed publisher
  24. Lawrence S, Luther K, Schindelin H, Ferry J. Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 2006;188:1143-54 pubmed publisher
    ..We propose that Arg310 binds acetyl phosphate and orients it for optimal nucleophilic attack. The hypothesized mechanism proceeds through a negatively charged transition state stabilized by hydrogen bond donation from Ser309...
  25. Ogawa T, Yoshimura T, Hemmi H. Connected cavity structure enables prenyl elongation across the dimer interface in mutated geranylfarnesyl diphosphate synthase from Methanosarcina mazei. Biochem Biophys Res Commun. 2011;409:333-7 pubmed publisher
    ..In particular, replacement of isoleucine 112 on the ?-helix greatly elongated the product chain-length, probably by connecting the reaction cavities of two subunits across the dimer interface. ..
  26. Yamada S, Niwa J, Ishigaki S, Takahashi M, Ito T, Sone J, et al. Archaeal proteasomes effectively degrade aggregation-prone proteins and reduce cellular toxicities in mammalian cells. J Biol Chem. 2006;281:23842-51 pubmed
    ..Our study showed that archaeal proteasomes can degrade aggregation-prone proteins whose toxic gain of function causes neurodegradation and reduce protein cellular toxicity. ..
  27. Corbett K, Benedetti P, Berger J. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nat Struct Mol Biol. 2007;14:611-9 pubmed publisher
    ..These data afford important insights into the mechanisms of topo VI and related proteins, including type IIA topoisomerases and the Spo11 meiotic recombination endonuclease...
  28. Manimekalai M, Kumar A, Balakrishna A, Gruber G. A second transient position of ATP on its trail to the nucleotide-binding site of subunit B of the motor protein A(1)A(0) ATP synthase. J Struct Biol. 2009;166:38-45 pubmed publisher
  29. SINGH D, Sielaff H, Börsch M, Gruber G. Conformational dynamics of the rotary subunit F in the A3 B3 DF complex of Methanosarcina mazei Gö1 A-ATP synthase monitored by single-molecule FRET. FEBS Lett. 2017;591:854-862 pubmed publisher
    ..We found two conformations for subunit F during ATP hydrolysis. ..
  30. Ronconi S, Jonczyk R, Genschel U. A novel isoform of pantothenate synthetase in the Archaea. FEBS J. 2008;275:2754-64 pubmed publisher
    ..These properties differ substantially from those of bacterial PS, and may explain why PS genes, in contrast to other pantothenate biosynthetic genes, were not exchanged horizontally between the Bacteria and Archaea...
  31. Beebe K, Mock M, Merriman E, Schimmel P. Distinct domains of tRNA synthetase recognize the same base pair. Nature. 2008;451:90-3 pubmed publisher
    ..These also recognize mischarged tRNA(Ala). Thus, through evolution, three different complexes with the same tRNA can guard against mistaking glycine or serine for alanine...
  32. Hausinger R, Moura I, Moura J, Xavier A, Santos M, LeGall J, et al. Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800). J Biol Chem. 1982;257:14192-7 pubmed
    ..This is the first sequence to be reported for a methanogen ferredoxin and only the fourth for a 3Fe:3S ferredoxin from any source. ..
  33. Thomsen J, De Biase A, Kaczanowski S, Macario A, Thomm M, Zielenkiewicz P, et al. The basal transcription factors TBP and TFB from the mesophilic archaeon Methanosarcina mazeii: structure and conformational changes upon interaction with stress-gene promoters. J Mol Biol. 2001;309:589-603 pubmed
  34. Chien Y, Zinder S. Cloning, functional organization, transcript studies, and phylogenetic analysis of the complete nitrogenase structural genes (nifHDK2) and associated genes in the archaeon Methanosarcina barkeri 227. J Bacteriol. 1996;178:143-8 pubmed
    ..These results support a model in which the nitrogenase structural genes in M. barkeri are cotranscribed in a single NH4(+)-repressed operon...
  35. Macario A, Simon V, Conway De Macario E. An archaeal gene upstream of grpE different from eubacterial counterparts. Biochim Biophys Acta. 1995;1264:173-7 pubmed
    ..Data from comparative analyses of the Orf16 deduced amino acid sequence and those of other known proteins, as well as the apparent biochemical characteristics of Orf16, suggest that the latter is a membrane molecule. ..
  36. Baumer S, Murakami E, Brodersen J, Gottschalk G, Ragsdale S, Deppenmeier U. The F420H2:heterodisulfide oxidoreductase system from Methanosarcina species. 2-Hydroxyphenazine mediates electron transfer from F420H2 dehydrogenase to heterodisulfide reductase. FEBS Lett. 1998;428:295-8 pubmed
    ..Thus, this water-soluble analogue of methanophenazine best mimics the natural electron acceptor methanophenazine in aqueous systems. ..
  37. Kiontke S, Gnau P, Haselsberger R, Batschauer A, Essen L. Structural and evolutionary aspects of antenna chromophore usage by class II photolyases. J Biol Chem. 2014;289:19659-69 pubmed publisher
    ..Overall, we suggest that 8-hydroxydeazaflavin is a crucial factor for the survival of most higher eukaryotes which depend on class II photolyases to struggle with the genotoxic effects of solar UV exposure. ..
  38. Wang S, Tiongson J, Rasche M. Discovery and characterization of the first archaeal dihydromethanopterin reductase, an iron-sulfur flavoprotein from Methanosarcina mazei. J Bacteriol. 2014;196:203-9 pubmed publisher
    ..These results provide the first biochemical evidence that MM1854 and MJ0208 function as archaeal dihydromethanopterin reductases (DmrX) and that ferredoxin may serve as an electron donor. ..
  39. Chartier F, Laine B, Belaiche D, Touzel J, Sautiere P. Primary structure of the chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55. Biochim Biophys Acta. 1989;1008:309-14 pubmed
    ..Transcription of DNA in vitro is stimulated by protein MC1 at low protein-to-DNA ratio but is inhibited at a ratio higher than 0.1 (w/w), which is the one determined in the bacterial deoxyribonucleoprotein complex. ..
  40. Weidenbach K, Ehlers C, Schmitz R. The transcriptional activator NrpA is crucial for inducing nitrogen fixation in Methanosarcina mazei Gö1 under nitrogen-limited conditions. FEBS J. 2014;281:3507-22 pubmed publisher
    ..mazei, nitrogen fixation is controlled by a hierarchical network of two transcriptional regulators, the global nitrogen repressor NrpR, and the newly identified activator NrpA (MM1708), thereby providing tight control of N2 fixation. ..
  41. Zmijewski M, Sk rko Glonek J, Tanfani F, Banecki B, Kotlarz A, Macario A, et al. The DnaK chaperones from the archaeon Methanosarcina mazei and the bacterium Escherichia coli have different substrate specificities. Acta Biochim Pol. 2007;54:509-22 pubmed
    ..Oligomers of DnaK(Mm) could be dissociated in the presence of ATP and a substrate (peptide) but not ADP, which may suggest that monomer is the active form of DnaK(Mm)...
  42. Welte C, Kröninger L, Deppenmeier U. Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei. FEMS Microbiol Lett. 2014;359:147-53 pubmed publisher
    ..These results show for the first time that an acetate transporter is involved in aceticlastic methanogenesis and may be an important factor in the acetate threshold concentration for methanogenesis of Methanosarcina spp. ..
  43. Welte C, Deppenmeier U. Re-evaluation of the function of the F420 dehydrogenase in electron transport of Methanosarcina mazei. FEBS J. 2011;278:1277-87 pubmed publisher
    ..mazei cytoplasm in considerable amounts. Consequently, soluble FpoF might participate in electron carrier equilibrium and facilitate survival of the Ms. mazei ?ech mutant that lacks the membrane-bound Fd-oxidizing Ech hydrogenase...
  44. Conway De Macario E, Dugan C, Macario A. Identification of a grpE heat-shock gene homolog in the archaeon Methanosarcina mazei. J Mol Biol. 1994;240:95-101 pubmed
    ..The M. mazei grpE responded to heat-shock by increasing transcription, in a manner similar to that of the nearby heat-shock gene dnaK...
  45. Chong Y, Yang X, Schimmel P. Natural homolog of tRNA synthetase editing domain rescues conditional lethality caused by mistranslation. J Biol Chem. 2008;283:30073-8 pubmed publisher
    ..The results support the idea that the unique widespread distribution of AlaXp arises from the singular difficulties, for translation, poised by alanine...
  46. Deppenmeier U, Blaut M, Lentes S, Herzberg C, Gottschalk G. Analysis of the vhoGAC and vhtGAC operons from Methanosarcina mazei strain Gö1, both encoding a membrane-bound hydrogenase and a cytochrome b. Eur J Biochem. 1995;227:261-9 pubmed
    ..Downstream of vhoC, a putative terminator sequence was identified. Alignments of the deduced amino acid sequences of the gene clusters vhoGAC and vhtGAC showed 92-97% identity. Only the C-termini of VhoC and VhtC were not similar...
  47. Imbert M, Laine B, Helbecque N, Mornon J, Henichart J, Sautiere P. Conformational study of the chromosomal protein MC1 from the archaebacterium Methanosarcina barkeri. Biochim Biophys Acta. 1990;1038:346-54 pubmed
    ..With respect to its primary structure and its conformation, protein MC1 appears radically different from the chromosomal DNA-binding protein II (also called HU-type protein) in eubacteria. ..
  48. Maupin Furlow J, Ferry J. Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. J Bacteriol. 1996;178:340-6 pubmed
    ..The purified CdhE protein exhibited an Fe-S center and base-off cobalamin binding in which the benzimidazole base nitrogen atom was no longer a lower axial ligand to the cobalt atom...
  49. Grahame D, DeMoll E. Partial reactions catalyzed by protein components of the acetyl-CoA decarbonylase synthase enzyme complex from Methanosarcina barkeri. J Biol Chem. 1996;271:8352-8 pubmed
    ..A value of 5.5 x [H+]-1 M-1 was determined for the equilibrium constant of the following reaction at pH 7.5 and 25 degrees C: CH3-H4SPt + cob(I)amide-protein + H+ <--> H4SPt + CH3-cob(III)amide-protein...
  50. Clements A, Ferry J. Cloning, nucleotide sequence, and transcriptional analyses of the gene encoding a ferredoxin from Methanosarcina thermophila. J Bacteriol. 1992;174:5244-50 pubmed
    ..The apparent transcriptional start sites of fdxA and ORF1, as determined by primer extension analyses, lay 21 to 28 bases downstream of sequences with high identity to the consensus methanogen promoter...
  51. Sabina J, S ll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006;281:6993-7001 pubmed publisher
    ..Whereas the C-terminal domains do not appear to play a role in selection of the modification site, their relevance for enzyme function and their role in vivo remains to be discovered...
  52. Hickey A, Macario A, Conway De Macario E. Identification of genes in the genome of the archaeon Methanosarcina mazeii that code for homologs of nuclear eukaryotic molecules involved in RNA processing. Gene. 2000;253:77-85 pubmed
    ..mazeii and the other archaeal molecules studied. The close proximity of the prp and fib genes raises the possibility of a Prp-fibrillarin interaction in archaea. ..
  53. Paul L, Ferguson D, Krzycki J. The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons. J Bacteriol. 2000;182:2520-9 pubmed
    ..Thus, the genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases...
  54. Balbo P, Oliveira M. Crystallization and preliminary X-ray data of the alpha2epsilon2 subcomponent of the acetyl-CoA decarbonylase/synthase multienzyme complex from Methanosarcina thermophila. Acta Crystallogr D Biol Crystallogr. 2003;59:721-3 pubmed
    ..The crystals were mounted in a capillary and diffracted to 4.0 A resolution at room temperature. Different flash-cooling approaches were attempted, all of which resulted in poor diffraction. ..
  55. Bycroft M, Bateman A, Clarke J, Hamill S, Sandford R, Thomas R, et al. The structure of a PKD domain from polycystin-1: implications for polycystic kidney disease. EMBO J. 1999;18:297-305 pubmed publisher
    ..This structure will allow the likely effects of missense mutations in a large part of the PKD1 gene to be determined...
  56. Springer T. An extracellular beta-propeller module predicted in lipoprotein and scavenger receptors, tyrosine kinases, epidermal growth factor precursor, and extracellular matrix components. J Mol Biol. 1998;283:837-62 pubmed publisher
    ..YWTD beta-propellers have a circular folding pattern that brings neighboring modules into close proximity, and may have important consequences for the architecture of multi-domain proteins...
  57. Lienard T, Gottschalk G. Cloning, sequencing and expression of the genes encoding the sodium translocating N5-methyltetrahydromethanopterin : coenzyme M methyltransferase of the methylotrophic archaeon Methanosarcina mazei Gö1. FEBS Lett. 1998;425:204-8 pubmed
    ..The participation of the gene product of mtrD in sodium ion translocation as well as a consensus sequence of a corrinoid binding motif in MtrA are discussed. ..
  58. Eggen R, van Kranenburg R, Vriesema A, Geerling A, Verhagen M, Hagen W, et al. Carbon monoxide dehydrogenase from Methanosarcina frisia Gö1. Characterization of the enzyme and the regulated expression of two operon-like cdh gene clusters. J Biol Chem. 1996;271:14256-63 pubmed
    ..The latter was, together with high-spin EPR signals of the oxidized enzyme tentatively assigned to an Fe/S cluster of high nuclearity...
  59. Ferguson D, Krzycki J. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri. J Bacteriol. 1997;179:846-52 pubmed
  60. Maupin Furlow J, Ferry J. Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Methanosarcina thermophila. J Bacteriol. 1996;178:6849-56 pubmed
    ..thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 from Methanobacterium ivanovii...
  61. Jovell R, Macario A, Conway De Macario E. ABC transporters in Archaea: two genes encoding homologs of the nucleotide-binding components in the methanogen Methanosarcina mazei S-6. Gene. 1996;174:281-4 pubmed
    ..These motifs, and other similarities of OrfD and OrfF with bacterial and eukaryal counterparts, indicate that the two archaeal molecules belong to the ATP-binding cassette (ABC)-transporter family...
  62. Kavran J, Gundllapalli S, O Donoghue P, Englert M, S ll D, Steitz T. Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation. Proc Natl Acad Sci U S A. 2007;104:11268-73 pubmed publisher
    ..The PylRS structure provides an excellent framework for designing new aaRSs with altered amino acid specificity...
  63. Malinen A, Belogurov G, Baykov A, Lahti R. Na+-pyrophosphatase: a novel primary sodium pump. Biochemistry. 2007;46:8872-8 pubmed publisher
    ..These findings define Na+-dependent PPases as electrogenic Na+ pumps. Phylogenetic analysis suggests that ancient gene duplication preceded the split of Na+- and H+-PPases...
  64. Rensing S, Maier U. Phylogenetic analysis of the stress-70 protein family. J Mol Evol. 1994;39:80-6 pubmed
    ..We present a phylogenetic tree containing all known stress-70 subfamilies and demonstrate the usefulness of stress-70 protein sequences for the estimation of intertaxonic phylogeny...
  65. Ogawa T, Yoshimura T, Hemmi H. Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution. Biochem Biophys Res Commun. 2010;393:16-20 pubmed publisher
  66. Jovell R, Macario A, Conway De Macario E. An ABC-transporter system homolog in an organism of the phylogenetic domain Archaea. DNA Seq. 1997;7:193-7 pubmed
    ..The proteins encoded by these genes were similar in structure, hydrophilicity-hydrophobicity profiles, and motifs to the equivalent components of homolog systems in bacteria and eucarya...
  67. Soares J, Zhang L, Pitsch R, Kleinholz N, Jones R, Wolff J, et al. The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases. J Biol Chem. 2005;280:36962-9 pubmed publisher
  68. Macario A, Dugan C, Conway De Macario E. An archaeal trkA homolog near dnaK and dnaJ. Biochim Biophys Acta. 1993;1216:495-8 pubmed
    ..e., that of the bacterium Escherichia coli, involved in K+ transport. This finding supports an essential, evolutionarily early, and conserved role for this gene in cell survival and adaptation. ..
  69. Yanagisawa T, Sumida T, Ishii R, Yokoyama S. A novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic site. Acta Crystallogr D Biol Crystallogr. 2013;69:5-15 pubmed publisher
    ..The orientation of the Asn346 side chain, which hydrogen-bonds to the carbonyl group of the amino-acid substrate, shifts by a maximum of 85-90° around the C(?) atom...
  70. Baumer S, Ide T, Jacobi C, Johann A, Gottschalk G, Deppenmeier U. The F420H2 dehydrogenase from Methanosarcina mazei is a Redox-driven proton pump closely related to NADH dehydrogenases. J Biol Chem. 2000;275:17968-73 pubmed publisher
  71. Tonouchi A, Nishizaki Y, Tohyama H, Takeda K. Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil. Curr Microbiol. 2002;45:390-3 pubmed
    ..The deduced amino acid sequence of the acetate kinase is 86.5% identical to that of the Methanosarcina thermophila acetate kinase. The activity of the His(6)-tagged acetate kinase purified from E. coli JM109 was optimal at 35 degrees C. ..
  72. Lienard T, Becher B, Marschall M, Bowien S, Gottschalk G. Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanosarcina mazei Gö1 reconstituted in ether lipid liposomes. Eur J Biochem. 1996;239:857-64 pubmed
    ..Methyltransferase activity was stimulated by sodium ions. 1.7 mol Na-/mol methyl groups transferred were translocated. Methyltetrahydrofolate and methyl-cobalamin could substitute for methyl-H,MPT...