Methanosarcina barkeri str. Fusaro

Summary

Alias: Methanosarcina barkeri 'Fusaro', Methanosarcina barkeri DSM 804, Methanosarcina barkeri Fusaro, Methanosarcina barkeri strain Fusaro

Top Publications

  1. Hao B, Gong W, Ferguson T, James C, Krzycki J, Chan M. A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science. 2002;296:1462-6 pubmed
    ..Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine. ..
  2. Srinivasan G, James C, Krzycki J. Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Science. 2002;296:1459-62 pubmed
    ..Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid...
  3. Polycarpo C, Ambrogelly A, Bérubé A, Winbush S, McCloskey J, Crain P, et al. An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. Proc Natl Acad Sci U S A. 2004;101:12450-4 pubmed
    ..This would ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguard the biosynthesis of the proteins whose genes contain this special codon...
  4. Burke S, Lo S, Krzycki J. Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine. J Bacteriol. 1998;180:3432-40 pubmed
    ..barkeri NIH. A mechanism that circumvents UAG-directed termination of translation must operate during expression of mtmB in this methanogen...
  5. Burke S, Krzycki J. Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 1997;272:16570-7 pubmed
    ..MMAMT functions as a MMA:MMCP methyltransferase, while MT2-A functions as a methyl-MMCP:CoM methyltransferase...
  6. Ferguson D, Gorlatova N, Grahame D, Krzycki J. Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 2000;275:29053-60 pubmed publisher
    ..These results indicate MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine...
  7. Paul L, Ferguson D, Krzycki J. The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons. J Bacteriol. 2000;182:2520-9 pubmed
    ..Thus, the genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases...
  8. Soares J, Zhang L, Pitsch R, Kleinholz N, Jones R, Wolff J, et al. The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases. J Biol Chem. 2005;280:36962-9 pubmed publisher
  9. Ferguson D, Krzycki J. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri. J Bacteriol. 1997;179:846-52 pubmed

More Information

Publications85

  1. Goenrich M, Thauer R, Yurimoto H, Kato N. Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis. Arch Microbiol. 2005;184:41-8 pubmed
    ..6 U/mg) and hexulose-6-phosphate synthase (Hps) activity (4.4 U/mg). The results support the recent proposal that in methanogenic archaea Fae and Hps could have a function in ribose phosphate synthesis. ..
  2. Bokranz M, Klein A. Nucleotide sequence of the methyl coenzyme M reductase gene cluster from Methanosarcina barkeri. Nucleic Acids Res. 1987;15:4350-1 pubmed
  3. Quitterer F, Beck P, Bacher A, Groll M. Structure and reaction mechanism of pyrrolysine synthase (PylD). Angew Chem Int Ed Engl. 2013;52:7033-7 pubmed publisher
  4. Moore S, Sowa S, Schuchardt C, Deery E, Lawrence A, Ramos J, et al. Elucidation of the biosynthesis of the methane catalyst coenzyme F430. Nature. 2017;543:78-82 pubmed publisher
    ..The research completes our understanding of how the repertoire of tetrapyrrole-based pigments are constructed, permitting the development of recombinant systems to use these metalloprosthetic groups more widely. ..
  5. Buchenau B, Thauer R. Tetrahydrofolate-specific enzymes in Methanosarcina barkeri and growth dependence of this methanogenic archaeon on folic acid or p-aminobenzoic acid. Arch Microbiol. 2004;182:313-25 pubmed publisher
    ..The presence of both H4SPT and H4F in M. barkeri is in agreement with earlier isotope labeling studies indicating that there are two separate C1 pools in these methanogens...
  6. Sauer K, Thauer R. Methanol:coenzyme M methyltransferase from Methanosarcina barkeri--identification of the active-site histidine in the corrinoid-harboring subunit MtaC by site-directed mutagenesis. Eur J Biochem. 1998;253:698-705 pubmed
    ..barkeri. M. barkeri contains an isoenzyme of MtaA designated MtbA. The isoenzyme reacted with MtaC with only 2.5% of the activity of MtaA. ..
  7. Kunow J, Shima S, Vorholt J, Thauer R. Primary structure and properties of the formyltransferase from the mesophilic Methanosarcina barkeri: comparison with the enzymes from thermophilic and hyperthermophilic methanogens. Arch Microbiol. 1996;165:97-105 pubmed
    ..Sequence similarities between the formyltransferases from methanogens and an open reading frame from Methylobacterium extorquens AM1 are discussed. ..
  8. Bertram P, Karrasch M, Schmitz R, Bocher R, Albracht S, Thauer R. Formylmethanofuran dehydrogenases from methanogenic Archaea. Substrate specificity, EPR properties and reversible inactivation by cyanide of the molybdenum or tungsten iron-sulfur proteins. Eur J Biochem. 1994;220:477-84 pubmed
    ..Inactivation was associated with a characteristic change in the molybdenum-derived EPR signal. Reactivation was possible in the presence of sulfide. ..
  9. Bilokapic S, Rokov Plavec J, Ban N, Weygand Durasevic I. Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition. FEBS J. 2008;275:2831-44 pubmed publisher
    ..In vitro kinetics results also suggest that conformational changes in the motif 2 loop are required for efficient serylation. ..
  10. Mukhopadhyay B, Purwantini E, Kreder C, Wolfe R. Oxaloacetate synthesis in the methanarchaeon Methanosarcina barkeri: pyruvate carboxylase genes and a putative Escherichia coli-type bifunctional biotin protein ligase gene (bpl/birA) exhibit a unique organization. J Bacteriol. 2001;183:3804-10 pubmed publisher
    ..We found the element Tyr(Phe)ProX(5)Phe(Tyr) to be fully conserved in biotin-dependent enzymes; it might function as the hinge for their "swinging arms."..
  11. Hausinger R, Moura I, Moura J, Xavier A, Santos M, LeGall J, et al. Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800). J Biol Chem. 1982;257:14192-7 pubmed
    ..This is the first sequence to be reported for a methanogen ferredoxin and only the fourth for a 3Fe:3S ferredoxin from any source. ..
  12. Krzycki J, Zeikus J. Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri. J Bacteriol. 1984;158:231-7 pubmed
    ..The spectral properties of the enzyme were similar to those published for CO dehydrogenase from acetogenic anaerobes. The physiological functions of the enzyme are discussed. ..
  13. Korencic D, Polycarpo C, Weygand Durasevic I, S ll D. Differential modes of transfer RNASer recognition in Methanosarcina barkeri. J Biol Chem. 2004;279:48780-6 pubmed publisher
    ..We propose that such a distinction between the two enzymes in tRNASer identity determinants reflects their evolutionary pathways, hence attesting to their diversity...
  14. Quitterer F, List A, Beck P, Bacher A, Groll M. Biosynthesis of the 22nd genetically encoded amino acid pyrrolysine: structure and reaction mechanism of PylC at 1.5Å resolution. J Mol Biol. 2012;424:270-82 pubmed publisher
    ..Whereas one ATP molecule is involved in catalysis, the second adenine nucleotide functions as a selective anchor for the C- and N-terminus of the Lys substrate and is responsible for protein stability as shown by mutagenesis. ..
  15. Ahel D, Slade D, Mocibob M, Soll D, Weygand Durasevic I. Selective inhibition of divergent seryl-tRNA synthetases by serine analogues. FEBS Lett. 2005;579:4344-8 pubmed
    ..Our kinetic data showed selective inhibition of the methanogenic SerRS by serinamide, suggesting a lack of mechanistic uniformity in serine recognition between the evolutionarily distinct SerRSs. ..
  16. Laine B, Chartier F, Imbert M, Lewis R, Sautiere P. Primary structure of the chromosomal protein HMb from the archaebacteria Methanosarcina barkeri. Eur J Biochem. 1986;161:681-7 pubmed
    ..8% of basic residues) which are distributed all along the polypeptide chain. The amino acid sequence of the protein HMb is not homologous to any eubacterial, archaebacterial or eukaryotic chromosomal proteins known up to now. ..
  17. Maupin Furlow J, Ferry J. A proteasome from the methanogenic archaeon Methanosarcina thermophila. J Biol Chem. 1995;270:28617-22 pubmed
    ..The results suggest that proteasomes are more widespread in the Archaea than previously proposed. Southern blotting experiments suggested the presence of ubiquitin-like sequences in M. thermophila. ..
  18. Kremer J, Cao X, Krzycki J. Isolation of two novel corrinoid proteins from acetate-grown Methanosarcina barkeri. J Bacteriol. 1993;175:4824-33 pubmed
    ..Both isolated proteins are abundant novel corrinoid proteins that can methylate and be methylated by intermediates of the methanogenic pathway. ..
  19. Tallant T, Krzycki J. Methylthiol:coenzyme M methyltransferase from Methanosarcina barkeri, an enzyme of methanogenesis from dimethylsulfide and methylmercaptopropionate. J Bacteriol. 1997;179:6902-11 pubmed
    ..These results indicate that the 480-kDa corrinoid protein functions as a CoM methylase during methanogenesis from DMS or MMPA. ..
  20. Harms U, Thauer R. Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum. Eur J Biochem. 1997;250:783-8 pubmed
    ..For comparison, the DNA sequences of the mtrEDCBAGH operon in M. kandleri and of the mtxXAH operon in M. barkeri were determined. ..
  21. Chartier F, Laine B, Belaiche D, Touzel J, Sautiere P. Primary structure of the chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55. Biochim Biophys Acta. 1989;1008:309-14 pubmed
    ..Transcription of DNA in vitro is stimulated by protein MC1 at low protein-to-DNA ratio but is inhibited at a ratio higher than 0.1 (w/w), which is the one determined in the bacterial deoxyribonucleoprotein complex. ..
  22. Quitterer F, List A, Eisenreich W, Bacher A, Groll M. Crystal structure of methylornithine synthase (PylB): insights into the pyrrolysine biosynthesis. Angew Chem Int Ed Engl. 2012;51:1339-42 pubmed publisher
  23. van der Meijden P, Te Brommelstroet B, Poirot C, van der Drift C, Vogels G. Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri. J Bacteriol. 1984;160:629-35 pubmed
    ..This activation was brought about by a mixture of H2, ferredoxin, and hydrogenase, but also by CO, which is thought to reduce the corrinoid chemically. The CO dehydrogenase-like activity of the methyltransferase is discussed. ..
  24. Brioukhanov A, Netrusov A, Eggen R. The catalase and superoxide dismutase genes are transcriptionally up-regulated upon oxidative stress in the strictly anaerobic archaeon Methanosarcina barkeri. Microbiology. 2006;152:1671-7 pubmed publisher
    ..PQ and to a lesser degree H2O2 induced the expression of superoxide dismutase. The results indicate the regulation of the adaptive response of M. barkeri to different oxidative stresses...
  25. Welander P, Metcalf W. Mutagenesis of the C1 oxidation pathway in Methanosarcina barkeri: new insights into the Mtr/Mer bypass pathway. J Bacteriol. 2008;190:1928-36 pubmed publisher
  26. Shima S, Netrusov A, Sordel M, Wicke M, Hartmann G, Thauer R. Purification, characterization, and primary structure of a monofunctional catalase from Methanosarcina barkeri. Arch Microbiol. 1999;171:317-23 pubmed
    ..Comparison of the primary structure of monofunctional catalases revealed that the enzyme from M. barkeri is most closely related to the monofunctional catalase of Dictyostelium discoideum...
  27. Kitabatake M, So M, Tumbula D, S ll D. Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?. J Bacteriol. 2000;182:143-5 pubmed
  28. Sauer K, Harms U, Thauer R. Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1. Eur J Biochem. 1997;243:670-7 pubmed
    ..They were found to be juxtapositioned and to form a transcription unit mtaCB. The corrinoid-harbouring subunit MtaC exhibits 35% sequence similarity to the cobalamin-binding domain of methionine synthase from Escherichia coli...
  29. Heiden S, Hedderich R, Setzke E, Thauer R. Purification of a two-subunit cytochrome-b-containing heterodisulfide reductase from methanol-grown Methanosarcina barkeri. Eur J Biochem. 1994;221:855-61 pubmed
    ..Upon addition of the heterodisulfide of coenzyme M and N-7-mercaptoheptanoylthreonine phosphate the cytochrome was instantaneously oxidized, indicating that the cytochrome b served as electron donor for heterodisulfide reduction...
  30. LeClerc G, Grahame D. Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression. J Biol Chem. 1996;271:18725-31 pubmed
    ..Active forms of both isozymes were expressed in Escherichia coli. A conserved segment with the potential for metal binding was found. The possibility of zinc involvement in catalysis of coenzyme M methylation is considered...
  31. Harms U, Thauer R. Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli. Eur J Biochem. 1996;235:653-9 pubmed
    ..coli. 25% of the E. coli protein was found to be active methyltransferase which could be purified in two steps to apparent homogeneity with a 70% yield...
  32. Tallant T, Krzycki J. Coenzyme M methylase activity of the 480-kilodalton corrinoid protein from Methanosarcina barkeri. J Bacteriol. 1996;178:1295-301 pubmed
    ..These data indicate that the 480-kDa corrinoid protein is composed of a novel isozyme of methyltransferase II which remains firmly bound to a corrinoid cofactor binding subunit during isolation...
  33. Vorholt J, Vaupel M, Thauer R. A polyferredoxin with eight [4Fe-4S] clusters as a subunit of molybdenum formylmethanofuran dehydrogenase from Methanosarcina barkeri. Eur J Biochem. 1996;236:309-17 pubmed
    ..A malE-fmdF gene fusion was constructed and expressed in E. coli, making the apoprotein of the polyferredoxin available in preparative amounts...
  34. Grabarse W, Mahlert F, Shima S, Thauer R, Ermler U. Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation. J Mol Biol. 2000;303:329-44 pubmed publisher
    ..This was reflected in a higher fraction of glutamate residues at the protein surface of the thermophilic enzymes adapted to high intracellular salt concentrations...
  35. Morris C, Reeve J. Conservation of structure in the human gene encoding argininosuccinate synthetase and the argG genes of the archaebacteria Methanosarcina barkeri MS and Methanococcus vannielii. J Bacteriol. 1988;170:3125-30 pubmed
  36. Boccazzi P, Zhang J, Metcalf W. Generation of dominant selectable markers for resistance to pseudomonic acid by cloning and mutagenesis of the ileS gene from the archaeon Methanosarcina barkeri fusaro. J Bacteriol. 2000;182:2611-8 pubmed
    ..PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-tRNA synthetase gene (ileS) from Methanosarcina barkeri Fusaro. The M...
  37. K nkel A, Vorholt J, Thauer R, Hedderich R. An Escherichia coli hydrogenase-3-type hydrogenase in methanogenic archaea. Eur J Biochem. 1998;252:467-76 pubmed
    ..coli hydrogenase-3-type hydrogenase also in these methanogenic archaea...
  38. Hagemeier C, Krer M, Thauer R, Warkentin E, Ermler U. Insight into the mechanism of biological methanol activation based on the crystal structure of the methanol-cobalamin methyltransferase complex. Proc Natl Acad Sci U S A. 2006;103:18917-22 pubmed publisher
    ..Implications of the MtaBC structure for the second step of the reaction, in which the methyl group is transferred to coenzyme M, are discussed...
  39. Sauer K, Thauer R. Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction. Eur J Biochem. 1997;249:280-5 pubmed
    ..0 kJ/mol. MtaB was found to contain 1 mol zinc, and its activity to be zinc dependent (pK(Zn2+) = 9.3). The zinc dependence of the MT2 (MtaA)-catalyzed reaction is also described (pK(Zn2+) = 9.6)...
  40. K nkel A, Vaupel M, Heim S, Thauer R, Hedderich R. Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri is not a flavoenzyme. Eur J Biochem. 1997;244:226-34 pubmed
  41. Bock A, Kunow J, Glasemacher J, Sch nheit P. Catalytic properties, molecular composition and sequence alignments of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro). Eur J Biochem. 1996;237:35-44 pubmed
    ..No sequence similarities were found, however, between the alpha-subunit of the M. barkeri enzyme and the heterodimeric pyruvate:ferredoxin oxidoreductase of the archaeon Halobacterium halobium...
  42. Storbeck S, Rolfes S, Raux Deery E, Warren M, Jahn D, Layer G. A novel pathway for the biosynthesis of heme in Archaea: genome-based bioinformatic predictions and experimental evidence. Archaea. 2010;2010:175050 pubmed publisher
  43. Metcalf W, Zhang J, Shi X, Wolfe R. Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro. J Bacteriol. 1996;178:5797-802 pubmed
    ..The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gene express phosphoserine aminotransferase activity, verifying the function of this gene...
  44. Ferguson D, Krzycki J, Grahame D. Specific roles of methylcobamide:coenzyme M methyltransferase isozymes in metabolism of methanol and methylamines in Methanosarcina barkeri. J Biol Chem. 1996;271:5189-94 pubmed
    ..The results establish that MT2-A plays a specific role in metabolism of methylated amine substrates, whereas, MT2-M functions in methane formation from trimethylamine and methanol...
  45. Polycarpo C, Ambrogelly A, Ruan B, Tumbula Hansen D, Ataide S, Ishitani R, et al. Activation of the pyrrolysine suppressor tRNA requires formation of a ternary complex with class I and class II lysyl-tRNA synthetases. Mol Cell. 2003;12:287-94 pubmed
    ..Structural modeling and selective inhibition experiments indicate that the class I and II LysRSs form a ternary complex with tRNAPyl, with the aminoacylation activity residing in the class II enzyme...
  46. Gencic S, Grahame D. Nickel in subunit beta of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. Catalytic properties and evidence for a binuclear Ni-Ni site. J Biol Chem. 2003;278:6101-10 pubmed
    ..A mechanism for C-C bond activation is proposed that includes a specific role for the Fe(4)S(4) center and accounts for the absolute requirement for nickel...
  47. Li T, Graham D, Stathopoulos C, Haney P, Kim H, Vothknecht U, et al. Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis. FEBS Lett. 1999;462:302-6 pubmed
    ..are specific relatives of the CysRS of Pyrococcus spp. and Chlamydia, respectively. This result suggests that the CysRS gene was acquired by lateral gene transfer in at least one euryarchaeotic lineage...
  48. Paul L, Krzycki J. Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase. J Bacteriol. 1996;178:6599-607 pubmed
    ..The implications of these homologies for the mechanism of corrinoid binding by proteins involved in methylotrophic methanogenesis are discussed...
  49. Gong W, Hao B, Wei Z, Ferguson D, Tallant T, Krzycki J, et al. Structure of the alpha2epsilon2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarbonylase/synthase complex. Proc Natl Acad Sci U S A. 2008;105:9558-63 pubmed publisher
  50. Bilokapic S, Maier T, Ahel D, Gruic Sovulj I, S ll D, Weygand Durasevic I, et al. Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition. EMBO J. 2006;25:2498-509 pubmed publisher
    ..Consequently, SerRS represents the only known aminoacyl-tRNA synthetase system that evolved two distinct mechanisms for the recognition of the same amino-acid substrate...
  51. Burke S, Krzycki J. Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine. J Bacteriol. 1995;177:4410-6 pubmed
    ..A likely mechanism is that the 29-kDa corrinoid is methylated by MMA and the methyl group is then transferred by the "A" isozyme of MT2 to CoM...
  52. Gaston M, Zhang L, Green Church K, Krzycki J. The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine. Nature. 2011;471:647-50 pubmed publisher
    ..Furthermore, intermediates of the pathway may provide new avenues by which the pyl system can be exploited to produce recombinant proteins with useful modified residues...
  53. Hyde S, Rao B, Eckenroth B, Jackman J, Doublié S. Structural studies of a bacterial tRNA(HIS) guanylyltransferase (Thg1)-like protein, with nucleotide in the activation and nucleotidyl transfer sites. PLoS ONE. 2013;8:e67465 pubmed publisher
    ..The BtTLP structural data, combined with kinetic analysis of selected variants, provide new insight into the role of key residues in the activation step. ..
  54. Thomas T, Cavicchioli R. Archaeal cold-adapted proteins: structural and evolutionary analysis of the elongation factor 2 proteins from psychrophilic, mesophilic and thermophilic methanogens. FEBS Lett. 1998;439:281-6 pubmed
  55. Breitung J, B rner G, Scholz S, Linder D, Stetter K, Thauer R. Salt dependence, kinetic properties and catalytic mechanism of N-formylmethanofuran:tetrahydromethanopterin formyltransferase from the extreme thermophile Methanopyrus kandleri. Eur J Biochem. 1992;210:971-81 pubmed
    ..The properties of the enzyme from M. kandleri are compared with those of formyltransferase from Methanobacterium thermoautotrophicum, Methanosarcina barkeri and Archaeoglobus fulgidus...
  56. Sibold L, Henriquet M, Possot O, Aubert J. Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes. Res Microbiol. 1991;142:5-12 pubmed
    ..These two ORF are followed by nifD in M. ivanovii as well as in the first nifH region of M. barkeri 227. Both types of ORF exhibit a strong homology with the glnB gene...
  57. Chien Y, Zinder S. Cloning, functional organization, transcript studies, and phylogenetic analysis of the complete nitrogenase structural genes (nifHDK2) and associated genes in the archaeon Methanosarcina barkeri 227. J Bacteriol. 1996;178:143-8 pubmed
    ..These results support a model in which the nitrogenase structural genes in M. barkeri are cotranscribed in a single NH4(+)-repressed operon...
  58. Chien Y, Auerbuch V, Brabban A, Zinder S. Analysis of genes encoding an alternative nitrogenase in the archaeon Methanosarcina barkeri 227. J Bacteriol. 2000;182:3247-53 pubmed
    ..barkeri 227, with one cluster resembling that from a gram-positive eubacterium and the other resembling a eubacterial V nitrogenase gene cluster, suggests horizontal genetic transfer of those genes...
  59. Sumi M, Yohda M, Koga Y, Yoshida M. F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri. Biochem Biophys Res Commun. 1997;241:427-33 pubmed publisher
    ..Attempts to detect F0F1-ATPase proteins and mRNA have been unsuccessful and therefore it is not certain if this gene cluster is really expressed in the cell...
  60. Rasche M, Smith K, Ferry J. Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 1997;179:7712-7 pubmed
    ..Five arginines were individually replaced with glutamine. Kinetic analysis of the altered enzymes identified R310 as essential for activity. Of the four nonessential for activity, R87 and R133 appear to be involved in binding CoA...
  61. Chien Y, Zinder S. Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum. J Bacteriol. 1994;176:6590-8 pubmed
    ..It is not clear whether this similarity is due to horizontal gene transfer or to the resemblance of the M. barkeri and C. pasteurianum nitrogenase sequences to an ancestral nitrogenase...
  62. Latimer M, Ferry J. Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila. J Bacteriol. 1993;175:6822-9 pubmed
  63. Lee H, Dominy B, Cao W. New family of deamination repair enzymes in uracil-DNA glycosylase superfamily. J Biol Chem. 2011;286:31282-7 pubmed publisher
    ..This study demonstrates the structural evolvability that underlies substrate specificity and catalytic flexibility in the evolution of enzymatic function...
  64. Maupin Furlow J, Ferry J. Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. J Bacteriol. 1996;178:340-6 pubmed
    ..The purified CdhE protein exhibited an Fe-S center and base-off cobalamin binding in which the benzimidazole base nitrogen atom was no longer a lower axial ligand to the cobalt atom...
  65. Grahame D, DeMoll E. Partial reactions catalyzed by protein components of the acetyl-CoA decarbonylase synthase enzyme complex from Methanosarcina barkeri. J Biol Chem. 1996;271:8352-8 pubmed
    ..A value of 5.5 x [H+]-1 M-1 was determined for the equilibrium constant of the following reaction at pH 7.5 and 25 degrees C: CH3-H4SPt + cob(I)amide-protein + H+ <--> H4SPt + CH3-cob(III)amide-protein...
  66. Gordiyenko Y, Videler H, Zhou M, McKay A, Fucini P, Biegel E, et al. Mass spectrometry defines the stoichiometry of ribosomal stalk complexes across the phylogenetic tree. Mol Cell Proteomics. 2010;9:1774-83 pubmed publisher
    ..Moreover the ratio of pentameric to heptameric stalk complexes changed during the course of cell growth. We consider these differences in stoichiometry within ribosomal stalk complexes in the context of convergent evolution. ..
  67. Maupin Furlow J, Ferry J. Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Methanosarcina thermophila. J Bacteriol. 1996;178:6849-56 pubmed
    ..thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 from Methanobacterium ivanovii...
  68. Iyer P, Lawrence S, Luther K, Rajashankar K, Yennawar H, Ferry J, et al. Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila. Structure. 2004;12:559-67 pubmed publisher
    ..A search for structural homologs revealed the NADP(+)-dependent isocitrate and isopropylmalate dehydrogenases as the only homologs with a similar two-domain architecture...
  69. Clements A, Ferry J. Cloning, nucleotide sequence, and transcriptional analyses of the gene encoding a ferredoxin from Methanosarcina thermophila. J Bacteriol. 1992;174:5244-50 pubmed
    ..The apparent transcriptional start sites of fdxA and ORF1, as determined by primer extension analyses, lay 21 to 28 bases downstream of sequences with high identity to the consensus methanogen promoter...
  70. Hippler B, Thauer R. The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH. FEBS Lett. 1999;449:165-8 pubmed
    ..Sequence comparison revealed similarity of MtrH with MetH from Escherichia coli and AcsE from Clostridium thermoaceticum: both enzymes exhibit methyltetrahydrofolate:cob(I)alamin methyltransferase activity...
  71. Sabina J, S ll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006;281:6993-7001 pubmed publisher
    ..Whereas the C-terminal domains do not appear to play a role in selection of the modification site, their relevance for enzyme function and their role in vivo remains to be discovered...
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    ..Conversely, the enzyme from M. barkeri and A. fulgidus already showed these properties, activity and stability, at much lower concentrations of these strong salting-out salts...
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    ..A classification scheme for chelatases is proposed...
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    ..Its structure suggests a different DNA-binding mode than those of the histone-like proteins HU or HMGB. Thus, MC1 may be classified as a member of a new family...