Methanosarcina acetivorans C2A


Alias: Methanosarcina acetivorans str. C2A

Top Publications

  1. Pritchett M, Metcalf W. Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A. Mol Microbiol. 2005;56:1183-94 pubmed publisher
    ..All deletion mutants were unaffected with respect to growth on trimethylamine and acetate corroborating biochemical evidence indicating that each methanogenic substrate has specific methyltransfer enzymes...
  2. Pritchett M, Zhang J, Metcalf W. Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea. Appl Environ Microbiol. 2004;70:1425-33 pubmed
    A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed...
  3. Morales R, Sriratana P, Zhang J, Cann I. Methanosarcina acetivorans C2A topoisomerase III?, an archaeal enzyme with promiscuity in divalent cation dependence. PLoS ONE. 2011;6:e26903 pubmed publisher
    ..Evidence that each of the split domains plays a role in Zn(2+) binding of the enzyme is also provided...
  4. Ferguson D, Gorlatova N, Grahame D, Krzycki J. Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri. J Biol Chem. 2000;275:29053-60 pubmed publisher
    ..These results indicate MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine...
  5. Soares J, Zhang L, Pitsch R, Kleinholz N, Jones R, Wolff J, et al. The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases. J Biol Chem. 2005;280:36962-9 pubmed publisher
  6. Longstaff D, Larue R, Faust J, Mahapatra A, Zhang L, Green Church K, et al. A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine. Proc Natl Acad Sci U S A. 2007;104:1021-6 pubmed
    ..Analogous cassettes may have served similar functions for other amino acids during the evolutionary expansion of the canonical genetic code. ..
  7. Ferguson D, Krzycki J. Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri. J Bacteriol. 1997;179:846-52 pubmed
  8. Bose A, Pritchett M, Metcalf W. Genetic analysis of the methanol- and methylamine-specific methyltransferase 2 genes of Methanosarcina acetivorans C2A. J Bacteriol. 2008;190:4017-26 pubmed publisher
    ..The mtaA1, mtaA2, and mtbA genes of Methanosarcina acetivorans C2A encode putative methanol- or methylamine-specific MT2 enzymes...
  9. Zhang J, White A, Kuettner H, Boccazzi P, Metcalf W. Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis. J Bacteriol. 2002;184:1449-54 pubmed
    We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the ..

More Information


  1. Paul L, Ferguson D, Krzycki J. The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons. J Bacteriol. 2000;182:2520-9 pubmed
    ..Thus, the genes encoding the three types of methyltransferases that initiate methanogenesis from methylamine contain in-frame amber codons that are suppressed during expression of the characterized methyltransferases...
  2. Nayak D, Metcalf W. Genetic techniques for studies of methyl-coenzyme M reductase from Methanosarcina acetivorans C2A. Methods Enzymol. 2018;613:325-347 pubmed publisher
    ..of a tandem affinity purification tag at the chromosomal mcr locus in the model methanogen, Methanosarcina acetivorans C2A. We also report a protocol for rapid affinity purification of MCR from M...
  3. Bose A, Kulkarni G, Metcalf W. Regulation of putative methyl-sulphide methyltransferases in Methanosarcina acetivorans C2A. Mol Microbiol. 2009;74:227-238 pubmed publisher
    ..Characterization of deletion mutants lacking putative regulatory genes suggests that MA0862 (msrF), MA4383 (msrC) and MA4560 (msrG) act as transcriptional activators of mtsD, mtsF and mtsH respectively. ..
  4. Chartier F, Laine B, Belaiche D, Touzel J, Sautiere P. Primary structure of the chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55. Biochim Biophys Acta. 1989;1008:309-14 pubmed
    ..Transcription of DNA in vitro is stimulated by protein MC1 at low protein-to-DNA ratio but is inhibited at a ratio higher than 0.1 (w/w), which is the one determined in the bacterial deoxyribonucleoprotein complex. ..
  5. Finn M, Tabita F. Modified pathway to synthesize ribulose 1,5-bisphosphate in methanogenic archaea. J Bacteriol. 2004;186:6360-6 pubmed publisher
    ..We suggest that the conversion of PRPP to RuBP might be an evolutional link between purine recycling pathways and the CBB scheme...
  6. Nardini M, Pesce A, Thijs L, Saito J, Dewilde S, Alam M, et al. Archaeal protoglobin structure indicates new ligand diffusion paths and modulation of haem-reactivity. EMBO Rep. 2008;9:157-63 pubmed publisher
    ..Protoglobin from Methanosarcina acetivorans C2A-a strictly anaerobic methanogenic Archaea-is, to the best of our knowledge, the latest entry adding ..
  7. Chien Y, Zinder S. Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum. J Bacteriol. 1994;176:6590-8 pubmed
    ..It is not clear whether this similarity is due to horizontal gene transfer or to the resemblance of the M. barkeri and C. pasteurianum nitrogenase sequences to an ancestral nitrogenase...
  8. Garc a Castellanos R, Tallant C, Marrero A, Sol M, Baumann U, Gomis R th F. Substrate specificity of a metalloprotease of the pappalysin family revealed by an inhibitor and a product complex. Arch Biochem Biophys. 2007;457:57-72 pubmed publisher
    ..The binding of batimastat to ulilysin evokes binding to vertebrate matrix metalloproteases but is much weaker. These data give insight into substrate specificity and mechanism of action and inhibition of the novel pappalysin family...
  9. Barnwal R, Jobby M, Devi K, Sharma Y, Chary K. Solution structure and calcium-binding properties of M-crystallin, a primordial betagamma-crystallin from archaea. J Mol Biol. 2009;386:675-89 pubmed publisher
    ..Additionally, identification of a betagamma-crystallin homologue in archaea allows us to demonstrate the presence of this domain in all the three domains of life...
  10. Graham D, Taylor S, Wolf R, Namboori S. Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase. Biochem J. 2009;424:467-78 pubmed publisher
    ..These differences in coenzyme M biosynthesis afford the opportunity to develop methanogen inhibitors that discriminate between the classes of methanogenic archaea...
  11. Gaston M, Zhang L, Green Church K, Krzycki J. The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine. Nature. 2011;471:647-50 pubmed publisher
    ..Furthermore, intermediates of the pathway may provide new avenues by which the pyl system can be exploited to produce recombinant proteins with useful modified residues...
  12. Lee H, Dominy B, Cao W. New family of deamination repair enzymes in uracil-DNA glycosylase superfamily. J Biol Chem. 2011;286:31282-7 pubmed publisher
    ..This study demonstrates the structural evolvability that underlies substrate specificity and catalytic flexibility in the evolution of enzymatic function...
  13. Aufhammer S, Warkentin E, Ermler U, Hagemeier C, Thauer R, Shima S. Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420: Architecture of the F420/FMN binding site of enzymes within the nonprolyl cis-peptide containing bacterial luciferase family. Protein Sci. 2005;14:1840-9 pubmed publisher
  14. Kliefoth M, Langer J, Matschiavelli N, Oelgeschläger E, Rother M. Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans. Arch Microbiol. 2012;194:75-85 pubmed publisher
    ..The data presented point toward a role of MA4079 to enable M. acetivorans to properly acclimate to CO. ..
  15. Ogawa T, Isobe K, Mori T, Asakawa S, Yoshimura T, Hemmi H. A novel geranylgeranyl reductase from the methanogenic archaeon Methanosarcina acetivorans displays unique regiospecificity. FEBS J. 2014;281:3165-76 pubmed publisher
    ..The available crystallographic structure of an orthologue enzyme may explain the reaction mechanism that achieves the substrate specificity and regiospecificity. ..
  16. Paquet F, Culard F, Barbault F, Maurizot J, Lancelot G. NMR solution structure of the archaebacterial chromosomal protein MC1 reveals a new protein fold. Biochemistry. 2004;43:14971-8 pubmed publisher
    ..Its structure suggests a different DNA-binding mode than those of the histone-like proteins HU or HMGB. Thus, MC1 may be classified as a member of a new family...
  17. Sabina J, S ll D. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. J Biol Chem. 2006;281:6993-7001 pubmed publisher
    ..Whereas the C-terminal domains do not appear to play a role in selection of the modification site, their relevance for enzyme function and their role in vivo remains to be discovered...
  18. Sibold L, Henriquet M, Possot O, Aubert J. Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes. Res Microbiol. 1991;142:5-12 pubmed
    ..These two ORF are followed by nifD in M. ivanovii as well as in the first nifH region of M. barkeri 227. Both types of ORF exhibit a strong homology with the glnB gene...
  19. Maupin Furlow J, Ferry J. Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. J Bacteriol. 1996;178:340-6 pubmed
    ..The purified CdhE protein exhibited an Fe-S center and base-off cobalamin binding in which the benzimidazole base nitrogen atom was no longer a lower axial ligand to the cobalt atom...
  20. Maupin Furlow J, Ferry J. A proteasome from the methanogenic archaeon Methanosarcina thermophila. J Biol Chem. 1995;270:28617-22 pubmed
    ..The results suggest that proteasomes are more widespread in the Archaea than previously proposed. Southern blotting experiments suggested the presence of ubiquitin-like sequences in M. thermophila. ..
  21. Laine B, Chartier F, Imbert M, Lewis R, Sautiere P. Primary structure of the chromosomal protein HMb from the archaebacteria Methanosarcina barkeri. Eur J Biochem. 1986;161:681-7 pubmed
    ..8% of basic residues) which are distributed all along the polypeptide chain. The amino acid sequence of the protein HMb is not homologous to any eubacterial, archaebacterial or eukaryotic chromosomal proteins known up to now. ..
  22. Rasche M, Smith K, Ferry J. Identification of cysteine and arginine residues essential for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 1997;179:7712-7 pubmed
    ..Five arginines were individually replaced with glutamine. Kinetic analysis of the altered enzymes identified R310 as essential for activity. Of the four nonessential for activity, R87 and R133 appear to be involved in binding CoA...
  23. Chien Y, Zinder S. Cloning, functional organization, transcript studies, and phylogenetic analysis of the complete nitrogenase structural genes (nifHDK2) and associated genes in the archaeon Methanosarcina barkeri 227. J Bacteriol. 1996;178:143-8 pubmed
    ..These results support a model in which the nitrogenase structural genes in M. barkeri are cotranscribed in a single NH4(+)-repressed operon...
  24. Latimer M, Ferry J. Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila. J Bacteriol. 1993;175:6822-9 pubmed
  25. Finn M, Tabita F. Synthesis of catalytically active form III ribulose 1,5-bisphosphate carboxylase/oxygenase in archaea. J Bacteriol. 2003;185:3049-59 pubmed
    ..fulgidus RubisCO possess unique properties with respect to quaternary structure, temperature optima, and activity in the presence of molecular oxygen compared to the previously described Thermococcus kodakaraensis and halophile proteins...
  26. Hausinger R, Moura I, Moura J, Xavier A, Santos M, LeGall J, et al. Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800). J Biol Chem. 1982;257:14192-7 pubmed
    ..This is the first sequence to be reported for a methanogen ferredoxin and only the fourth for a 3Fe:3S ferredoxin from any source. ..
  27. Besserer G, Nicoll D, Abramson J, Philipson K. Characterization and purification of a Na+/Ca2+ exchanger from an archaebacterium. J Biol Chem. 2012;287:8652-9 pubmed publisher
    ..The availability of a prokaryotic Na(+)/Ca(2+) exchanger should facilitate structural and mechanistic investigations. ..
  28. Vepachedu V, Ferry J. Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans. J Bacteriol. 2012;194:4161-8 pubmed publisher
    ..acetivorans. Thus, we propose that the name of the enzyme encoded by MA4384 be CmtA (for cytoplasmic methyltransferase). ..
  29. Molitor B, Stassen M, Modi A, El Mashtoly S, Laurich C, Lubitz W, et al. A heme-based redox sensor in the methanogenic archaeon Methanosarcina acetivorans. J Biol Chem. 2013;288:18458-72 pubmed publisher
    ..On the basis of our results, we postulate a heme-based redox/dimethyl sulfide sensory function of MA4561 and propose to designate it MsmS (methyl sulfide methyltransferase-associated sensor). ..
  30. Ascenzi P, Leboffe L, Pesce A, Ciaccio C, Sbardella D, Bolognesi M, et al. Nitrite-reductase and peroxynitrite isomerization activities of Methanosarcina acetivorans protoglobin. PLoS ONE. 2014;9:e95391 pubmed publisher
    ..These results highlight the potential role of Pgbs in the biosynthesis and scavenging of reactive nitrogen and oxygen species. ..
  31. Imbert M, Laine B, Helbecque N, Mornon J, Henichart J, Sautiere P. Conformational study of the chromosomal protein MC1 from the archaebacterium Methanosarcina barkeri. Biochim Biophys Acta. 1990;1038:346-54 pubmed
    ..With respect to its primary structure and its conformation, protein MC1 appears radically different from the chromosomal DNA-binding protein II (also called HU-type protein) in eubacteria. ..
  32. Wang P, Sun G, Zhu Y. Identification and characterization of arsenite methyltransferase from an archaeon, Methanosarcina acetivorans C2A. Environ Sci Technol. 2014;48:12706-13 pubmed publisher
    ..Here, an arsenite [As(III)] methyltransferase (ArsM) was identified and characterized from an archaeon Methanosarcina acetivorans C2A. Heterologous expression of MaarsM was shown to confer As(III) resistance to an arsenic-sensitive ..
  33. Fu H, Metcalf W. Genetic basis for metabolism of methylated sulfur compounds in Methanosarcina species. J Bacteriol. 2015;197:1515-24 pubmed publisher
    ..These data will aid the development of predictive sulfur cycle models and enable molecular ecological approaches for the study of methylated sulfur metabolism in anaerobic ecosystems. ..
  34. Li J, Guo J, Ou X, Zhang M, Li Y, Liu Z. Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion. Proc Natl Acad Sci U S A. 2015;112:10726-31 pubmed publisher
    ..These results provide direct mechanistic insights into the highly coordinated movement of the different domains of the MscL channel when it expands. ..
  35. Fay A, Wiig J, Lee C, Hu Y. Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens. Proc Natl Acad Sci U S A. 2015;112:14829-33 pubmed publisher
  36. Rauch B, Perona J. Efficient Sulfide Assimilation in Methanosarcina acetivorans Is Mediated by the MA1715 Protein. J Bacteriol. 2016;198:1974-83 pubmed publisher
    ..The newly characterized genes thus likely formed an essential part of the core metabolic machinery of the ancestral euryarchaeote. ..
  37. Zheng K, Ngo P, Owens V, Yang X, Mansoorabadi S. The biosynthetic pathway of coenzyme F430 in methanogenic and methanotrophic archaea. Science. 2016;354:339-342 pubmed identify the coenzyme F430 biosynthesis (cfb) genes and characterized the encoded enzymes from Methanosarcina acetivorans C2A. The pathway involves nickelochelation by a nickel-specific chelatase, followed by amidation to form ..
  38. Chong Y, Yang X, Schimmel P. Natural homolog of tRNA synthetase editing domain rescues conditional lethality caused by mistranslation. J Biol Chem. 2008;283:30073-8 pubmed publisher
    ..The results support the idea that the unique widespread distribution of AlaXp arises from the singular difficulties, for translation, poised by alanine...
  39. Oelgeschl ger E, Rother M. In vivo role of three fused corrinoid/methyl transfer proteins in Methanosarcina acetivorans. Mol Microbiol. 2009;72:1260-72 pubmed publisher
    ..acetivorans. Based on these findings we propose to designate MA0859, MA4384 and MA4558 as methyltransferases specific for methylsulphides, MtsD, MtsF and MtsH respectively...
  40. Gerber S, Comellas Bigler M, Goetz B, Locher K. Structural basis of trans-inhibition in a molybdate/tungstate ABC transporter. Science. 2008;321:246-50 pubmed publisher
    ..This allosteric effect prevents the transporter from switching between the inward-facing and the outward-facing states, thus interfering with the alternating access and release mechanism...
  41. Grahame D, DeMoll E. Partial reactions catalyzed by protein components of the acetyl-CoA decarbonylase synthase enzyme complex from Methanosarcina barkeri. J Biol Chem. 1996;271:8352-8 pubmed
    ..A value of 5.5 x [H+]-1 M-1 was determined for the equilibrium constant of the following reaction at pH 7.5 and 25 degrees C: CH3-H4SPt + cob(I)amide-protein + H+ <--> H4SPt + CH3-cob(III)amide-protein...
  42. Boocock G, Morrison J, Popovic M, Richards N, Ellis L, Durie P, et al. Mutations in SBDS are associated with Shwachman-Diamond syndrome. Nat Genet. 2003;33:97-101 pubmed publisher
  43. Lessner D, Ferry J. The archaeon Methanosarcina acetivorans contains a protein disulfide reductase with an iron-sulfur cluster. J Bacteriol. 2007;189:7475-84 pubmed publisher
  44. Sumi M, Yohda M, Koga Y, Yoshida M. F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri. Biochem Biophys Res Commun. 1997;241:427-33 pubmed publisher
    ..Attempts to detect F0F1-ATPase proteins and mRNA have been unsuccessful and therefore it is not certain if this gene cluster is really expressed in the cell...
  45. Pandey D, Gerdes K. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res. 2005;33:966-76 pubmed publisher
  46. Tonouchi A, Nishizaki Y, Tohyama H, Takeda K. Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil. Curr Microbiol. 2002;45:390-3 pubmed
    ..The deduced amino acid sequence of the acetate kinase is 86.5% identical to that of the Methanosarcina thermophila acetate kinase. The activity of the His(6)-tagged acetate kinase purified from E. coli JM109 was optimal at 35 degrees C. ..
  47. Lawrence S, Luther K, Schindelin H, Ferry J. Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila. J Bacteriol. 2006;188:1143-54 pubmed publisher
    ..We propose that Arg310 binds acetyl phosphate and orients it for optimal nucleophilic attack. The hypothesized mechanism proceeds through a negatively charged transition state stabilized by hydrogen bond donation from Ser309...
  48. Iyer P, Lawrence S, Luther K, Rajashankar K, Yennawar H, Ferry J, et al. Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila. Structure. 2004;12:559-67 pubmed publisher
    ..A search for structural homologs revealed the NADP(+)-dependent isocitrate and isopropylmalate dehydrogenases as the only homologs with a similar two-domain architecture...
  49. Gencic S, Grahame D. Nickel in subunit beta of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. Catalytic properties and evidence for a binuclear Ni-Ni site. J Biol Chem. 2003;278:6101-10 pubmed
    ..A mechanism for C-C bond activation is proposed that includes a specific role for the Fe(4)S(4) center and accounts for the absolute requirement for nickel...
  50. Yenugudhati D, Prakash D, Kumar A, Kumar R, Yennawar N, Yennawar H, et al. Structural and Biochemical Characterizations of Methanoredoxin from Methanosarcina acetivorans, a Glutaredoxin-Like Enzyme with Coenzyme M-Dependent Protein Disulfide Reductase Activity. Biochemistry. 2016;55:313-21 pubmed publisher
    ..The results, combined with our bioinformatic analyses, show that MRX represents a class of GRX-like enzymes present in a diversity of methane-producing Archaea. ..
  51. Chien Y, Auerbuch V, Brabban A, Zinder S. Analysis of genes encoding an alternative nitrogenase in the archaeon Methanosarcina barkeri 227. J Bacteriol. 2000;182:3247-53 pubmed
    ..barkeri 227, with one cluster resembling that from a gram-positive eubacterium and the other resembling a eubacterial V nitrogenase gene cluster, suggests horizontal genetic transfer of those genes...
  52. Thomas T, Cavicchioli R. Archaeal cold-adapted proteins: structural and evolutionary analysis of the elongation factor 2 proteins from psychrophilic, mesophilic and thermophilic methanogens. FEBS Lett. 1998;439:281-6 pubmed
  53. Clements A, Ferry J. Cloning, nucleotide sequence, and transcriptional analyses of the gene encoding a ferredoxin from Methanosarcina thermophila. J Bacteriol. 1992;174:5244-50 pubmed
    ..The apparent transcriptional start sites of fdxA and ORF1, as determined by primer extension analyses, lay 21 to 28 bases downstream of sequences with high identity to the consensus methanogen promoter...
  54. Jasso Ch vez R, Apolinario E, Sowers K, Ferry J. MrpA functions in energy conversion during acetate-dependent growth of Methanosarcina acetivorans. J Bacteriol. 2013;195:3987-94 pubmed publisher
    ..The results indicated that the Mrp complex is essential for efficient ATP synthesis and optimal growth at the low concentrations of acetate encountered in the environment...
  55. Tallant C, García Castellanos R, Seco J, Baumann U, Gomis Ruth F. Molecular analysis of ulilysin, the structural prototype of a new family of metzincin metalloproteases. J Biol Chem. 2006;281:17920-8 pubmed
    ..Accordingly, ulilysin has higher proteolytic efficiency and a broader substrate specificity than human PAPP-A. The structure of ulilysin represents a prototype for the catalytic domain of pappalysins. ..
  56. Reichlen M, Vepachedu V, Murakami K, Ferry J. MreA functions in the global regulation of methanogenic pathways in Methanosarcina acetivorans. MBio. 2012;3:e00189-12 pubmed publisher
    ..Finally, the results contribute to a broader understanding of transcriptional regulation in the domain Archaea...
  57. Inatomi K, Eya S, Maeda M, Futai M. Amino acid sequence of the alpha and beta subunits of Methanosarcina barkeri ATPase deduced from cloned genes. Similarity to subunits of eukaryotic vacuolar and F0F1-ATPases. J Biol Chem. 1989;264:10954-9 pubmed
    ..coli enzyme were conserved in the methanogenic enzyme. This sequence conservation suggests that vacuolar, F1, methanogenic, and S. acidocaldarius ATPases were derived from a common ancestral enzyme. ..
  58. Kumar A, Yennawar N, Yennawar H, Ferry J. Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a novel plant-type ferredoxin/thioredoxin reductase-like protein from Methanosarcina acetivorans. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:775-8 pubmed publisher
    ..72 Å. Assuming the presence of one molecule in the asymmetric unit gave a Matthews coefficient (VM) of 3.55 Å3 Da(-1), corresponding to a solvent content of 65%. ..
  59. Maupin Furlow J, Ferry J. Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Methanosarcina thermophila. J Bacteriol. 1996;178:6849-56 pubmed
    ..thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 from Methanobacterium ivanovii...
  60. Barnwal R, Devi K, Agarwal G, Sharma Y, Chary K. Temperature-dependent oligomerization in M-crystallin: lead or lag toward cataract, an NMR perspective. Proteins. 2011;79:569-80 pubmed publisher
    ..These higher states may co-exist in fast exchange with the monomeric and dimeric M-crystallin at milli-molar to sub-millimolar concentrations and at lower temperature...