Methanothermobacter thermautotrophicus str. Delta H

Summary

Alias: Methanobacterium thermoautotrophicum str. Delta H, Methanothermobacter thermautotrophicus str. deltaH, Methanobacterium thermoautotrophicum str. deltaH

Top Publications

  1. Tumbula D, Becker H, Chang W, Soll D. Domain-specific recruitment of amide amino acids for protein synthesis. Nature. 2000;407:106-10 pubmed
    ..Closer inspection of the two amidotransferases reveals that each of them recruited a metabolic enzyme to aid its function; this provides direct evidence for a relationship between amino-acid metabolism and protein biosynthesis. ..
  2. Bolt E, Lloyd R, Sharples G. Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli. J Mol Biol. 2001;310:577-89 pubmed
    ..These results support models in which RecG acts at a replication fork stalled at a lesion in the DNA, catalysing fork regression and forming a Holliday junction that can then be acted upon by Hjc. ..
  3. Grabowski B, Kelman Z. Autophosphorylation of archaeal Cdc6 homologues is regulated by DNA. J Bacteriol. 2001;183:5459-64 pubmed
    ..Yeast Cdc18 was also found to autophosphorylate, suggesting that this function of Cdc6 may play a widely conserved and essential role in replication initiation. ..
  4. Fletcher R, Bishop B, Leon R, Sclafani R, Ogata C, Chen X. The structure and function of MCM from archaeal M. Thermoautotrophicum. Nat Struct Biol. 2003;10:160-7 pubmed
    ..Yeast bypass experiments using MCM5 mutant proteins support the hypothesis for the bypass mechanism. ..
  5. Kelman Z, Hurwitz J. A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH. J Biol Chem. 2000;275:7327-36 pubmed
    ..The importance and roles of RFC and PCNA in M. thermoautotrophicum DeltaH replication are discussed...
  6. Capaldi S, Berger J. Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus. Nucleic Acids Res. 2004;32:4821-32 pubmed
    ..Our observations also indicate that the origin-binding properties of Cdc6/Orc1 proteins closely resemble those of bacterial DnaA...
  7. Chen Y, Yu X, Kasiviswanathan R, Shin J, Kelman Z, Egelman E. Structural polymorphism of Methanothermobacter thermautotrophicus MCM. J Mol Biol. 2005;346:389-94 pubmed
    ..This offers new insight into the conformational dynamics of MCM and the phosphorylation-bypass phenotype in yeast...
  8. Pfeifer S, Greiner Stöffele T. A recombinant exonuclease III homologue of the thermophilic archaeon Methanothermobacter thermautotrophicus. DNA Repair (Amst). 2005;4:433-44 pubmed
    ..Considering the high intracellular potassium ion concentration in M. thermautotrophicus, our results suggest that the characterized thermophilic enzyme acts as an AP endonuclease in vivo with similar activities as Ape1...
  9. Ng C, Waterman D, Antson A, Ortiz Lombardía M. Structure of the Methanothermobacter thermautotrophicus exosome RNase PH ring. Acta Crystallogr D Biol Crystallogr. 2010;66:522-8 pubmed publisher

More Information

Publications149 found, 100 shown here

  1. Fujihashi M, Bello A, Poduch E, Wei L, Annedi S, Pai E, et al. An unprecedented twist to ODCase catalytic activity. J Am Chem Soc. 2005;127:15048-50 pubmed
    ..This potential of ODCase is very useful in the design of novel inhibitors. ..
  2. NOLLING J, van Eeden F, de Vos W. Distribution and characterization of plasmid-related sequences in the chromosomal DNA of different thermophilic Methanobacterium strains. Mol Gen Genet. 1993;240:81-91 pubmed
    ..5-3.0 kb in size and contained large open reading frames (ORFs) that may encode highly related proteins with an as yet unknown function. ..
  3. Kamtekar S, Kennedy W, Wang J, Stathopoulos C, S ll D, Steitz T. The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases. Proc Natl Acad Sci U S A. 2003;100:1673-8 pubmed publisher
    ..jannaschii ProRS misacylates tRNA(Pro) with cysteine, and argue against the proposal that these archaeal ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cognate amino acids...
  4. Tao X, Khayat R, Christendat D, Savchenko A, Xu X, Goldsmith Fischman S, et al. Crystal structures of MTH1187 and its yeast ortholog YBL001c. Proteins. 2003;52:478-80 pubmed publisher
  5. Ye S, Li Y, Chen L, Jiang Y. Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channels. Cell. 2006;126:1161-73 pubmed publisher
    ..Our findings, along with the previously determined open MthK structure, allow us to elucidate the ligand gating mechanism of RCK-regulated K(+) channels...
  6. Takao M, Oikawa A, Yasui A. Characterization of a superoxide dismutase gene from the archaebacterium Methanobacterium thermoautotrophicum. Arch Biochem Biophys. 1990;283:210-6 pubmed
    ..Between a putative promoter and the start codon there is an inverted repeat sequence which is also found in the counterpart of Halobacterium halobium. ..
  7. Yee A, Booth V, Dharamsi A, Engel A, Edwards A, Arrowsmith C. Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 2000;97:6311-5 pubmed
    ..The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein...
  8. Renault J, Verchère Béaur C, Morgenstern Badarau I, Yamakura F, Gerloch M. EPR and ligand field studies of iron superoxide dismutases and iron-substituted manganese superoxide dismutases: relationships between electronic structure of the active site and activity. Inorg Chem. 2000;39:2666-75 pubmed
    ..We conjecture that such coupling should be considered in the electron-transfer process and in the contribution of the typical electronic structure of FeSOD to the activity. ..
  9. Fay A, Wiig J, Lee C, Hu Y. Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens. Proc Natl Acad Sci U S A. 2015;112:14829-33 pubmed publisher
  10. Berghöfer B, Krockel L, Körtner C, Truss M, Schallenberg J, Klein A. Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalents. Nucleic Acids Res. 1988;16:8113-28 pubmed
    ..Putative functional domains were identified in two of the subunits of the archaebacterial enzyme. ..
  11. Saridakis V, Christendat D, Kimber M, Dharamsi A, Edwards A, Pai E. Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes. J Biol Chem. 2001;276:7225-32 pubmed publisher
    ..In addition, the role of the conserved (16)HXGH(19) active site motif in catalysis was probed by mutagenic, enzymatic and crystallographic techniques, including the characterization of an NMN(+)/SO4(2-) complex of mutant H19A NMNATase...
  12. Georg J, Schomacher L, Chong J, Majern k A, Raabe M, Urlaub H, et al. The Methanothermobacter thermautotrophicus ExoIII homologue Mth212 is a DNA uridine endonuclease. Nucleic Acids Res. 2006;34:5325-36 pubmed publisher
    ..The U-endo activity of Mth212 is served by the same catalytic center as its AP-endo activity...
  13. Hudson A, Gilvarg C, Leustek T. Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase. J Bacteriol. 2008;190:3256-63 pubmed publisher
  14. Meile L, Stettler R, Banholzer R, Kotik M, Leisinger T. Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operon. J Bacteriol. 1991;173:5017-23 pubmed
    ..With the exception of TrpB, the beta subunit of tryptophan synthase, tryptophan was absent from all Trp polypeptides. ..
  15. Vaupel M, Thauer R. Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase (Mer) from Methanobacterium thermoautotrophicum strain Marburg. Cloning, sequencing, transcriptional analysis, and functional expression in Escherichia coli of the mer gene. Eur J Biochem. 1995;231:773-8 pubmed
    ..Sequence comparisons revealed similarities between the F420-dependent N5,N10-methylenetetrahydromethanopterin reductase and a F420-dependent reductase involved in lincomycin biosynthesis in Streptomyces lincolnensis. ..
  16. Kuhner C, Lindenbach B, Wolfe R. Component A2 of methylcoenzyme M reductase system from Methanobacterium thermoautotrophicum delta H: nucleotide sequence and functional expression by Escherichia coli. J Bacteriol. 1993;175:3195-203 pubmed
    ..2-kb XmaI-BamHI fragment containing atwA and the surrounding open reading frames was cloned into pGEM-7Zf(+). A cell extract from this strain replaced purified A2 from M. thermoautotrophicum delta H in an in vitro methylreductase assay...
  17. Kim H, Vothknecht U, Hedderich R, Celic I, Soll D. Sequence divergence of seryl-tRNA synthetases in archaea. J Bacteriol. 1998;180:6446-9 pubmed
    ..Gel shift experiments showed that M. thermoautotrophicum SerRS did not mischarge tRNACys with serine. This indicates that Cys-tRNACys is formed by direct acylation in these organisms. ..
  18. Das R, Vothknecht U. Phenylalanyl-tRNA synthetase from the archaeon Methanobacterium thermoautotrophicum is an (alphabeta)2 heterotetrameric protein. Biochimie. 1999;81:1037-9 pubmed
    ..Two ORFs with similarity to non-archaeal PheRSs alpha-subunits had previously been found in the genome sequence, but these results show that only one of them, MT742, is part of the active PheRS. ..
  19. Bhattacharyya S, Habibi Nazhad B, Amegbey G, Slupsky C, Yee A, Arrowsmith C, et al. Identification of a novel archaebacterial thioredoxin: determination of function through structure. Biochemistry. 2002;41:4760-70 pubmed
    ..On the basis of the results presented here, we predict that these small proteins are all members of a new class of truncated thioredoxins...
  20. Jiang Y, Lee A, Chen J, Cadene M, Chait B, MacKinnon R. Crystal structure and mechanism of a calcium-gated potassium channel. Nature. 2002;417:515-22 pubmed publisher
    ..Eight RCK domains (regulators of K(+) conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca(2+) binding in a simple manner to perform mechanical work to open the pore...
  21. Blanco F, Yee A, Campos Olivas R, Ortiz A, Devos D, Valencia A, et al. Solution structure of the hypothetical protein Mth677 from Methanobacterium thermoautotrophicum: a novel alpha+beta fold. Protein Sci. 2004;13:1458-65 pubmed
    ..coli. ..
  22. Ko S, Kim H, Yun J, Yee A, Arrowsmith C, Cheong C, et al. Solution structure of MTH1821, a putative structure homologue to RNA polymerase ? subunit from Methanobacterium thermoautotrophicum. Proteins. 2011;79:1347-51 pubmed publisher
  23. Smith F, Pau V, Cingolani G, Rothberg B. Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation. Structure. 2012;20:2038-47 pubmed publisher
    ..Our results suggest a working hypothesis for a sequence of ligand-dependent conformational changes that may underlie RCK domain activation and channel gating. ..
  24. Matheson A, Yaguchi M, Balch W, Wolfe R. Sequence homologies in the N-terminal region of the ribosomal 'A' proteins from Methanobacterium Thermoautotrophicum and Halobacterium cutirubrum. Biochim Biophys Acta. 1980;626:162-9 pubmed
    ..The sequence data from the 'A' proteins also indicate that, phylogenetically, the archaebacteria are much closer to the cytoplasmic components of eucaryotes than they are to the eubacteria (or 'true bacteria'). ..
  25. Renault J, Morgenstern Badarau I, Piccioli M. Thermochromic Conformational Change of Methanobacterium thermoautotrophicum Iron Superoxide Dismutase. Inorg Chem. 1999;38:614-615 pubmed
  26. Mura C, Kozhukhovsky A, Gingery M, Phillips M, Eisenberg D. The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs). Protein Sci. 2003;12:832-47 pubmed publisher
    ..Finally, we found that both Pae and Mth SmAP1 gel-shift negatively supercoiled DNA. These results distinguish SmAPs from eukaryotic Sm proteins and suggest that SmAPs have a generic single-stranded nucleic acid-binding activity...
  27. Canaves J. Predicted role for the archease protein family based on structural and sequence analysis of TM1083 and MTH1598, two proteins structurally characterized through structural genomics efforts. Proteins. 2004;56:19-27 pubmed publisher
    ..The conservation of genomic context for this protein family is constant from Archaea and Bacteria to humans, and suggests that unannotated open reading frames contiguous to them could be novel RNA/DNA binding proteins...
  28. Kasiviswanathan R, Shin J, Kelman Z. DNA binding by the Methanothermobacter thermautotrophicus Cdc6 protein is inhibited by the minichromosome maintenance helicase. J Bacteriol. 2006;188:4577-80 pubmed publisher
    ..Using minichromosome maintenance (MCM) helicase mutant proteins unable to bind DNA, it was found that the interaction of MCM with Cdc6 inhibits the DNA binding activity of Cdc6...
  29. Lakomek K, Dickmanns A, Ciirdaeva E, Schomacher L, Ficner R. Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA. J Mol Biol. 2010;399:604-17 pubmed publisher
    ..In addition, Ser171, Asn153, and Lys125 in the substrate binding pocket appear to have important functions in the discrimination of aberrant uridine against naturally occurring thymidine and cytosine residues in double-stranded DNA. ..
  30. Ingram Smith C, Thurman J, Zimowski K, Smith K. Role of motif III in catalysis by acetyl-CoA synthetase. Archaea. 2012;2012:509579 pubmed publisher
    ..Interactions between these Asp and ATP are observed in all structures available for members of the superfamily, consistent with a critical role in substrate binding and catalysis for this invariant residue. ..
  31. Hedderich R, Berkessel A, Thauer R. Purification and properties of heterodisulfide reductase from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1990;193:255-61 pubmed
    ..2 mM) plus H-S-HTP (app. Km less than 0.05 mM) at a specific rate of 15 mumol.min-1.mg-1. The enzyme was highly specific for CoM-S-S-HTP and H-S-CoM plus H-S-HTP. The physiological electron donor/acceptor remains to be identified. ..
  32. Knaub S, Klein A. Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro. Nucleic Acids Res. 1990;18:1441-6 pubmed
    ..The 5'-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function. ..
  33. Koga -, Kyuragi -, Nishihara -, Sone -. Did archaeal and bacterial cells arise independently from noncellular Precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent. J Mol Evol. 1998;47:631 pubmed
  34. Mura C, Cascio D, Sawaya M, Eisenberg D. The crystal structure of a heptameric archaeal Sm protein: Implications for the eukaryotic snRNP core. Proc Natl Acad Sci U S A. 2001;98:5532-7 pubmed publisher
    ..The pronounced electrostatic asymmetry of the SmAP surface imparts directionality to putative SmAP-RNA interactions...
  35. Zarembinski T, Kim Y, Peterson K, Christendat D, Dharamsi A, Arrowsmith C, et al. Deep trefoil knot implicated in RNA binding found in an archaebacterial protein. Proteins. 2003;50:177-83 pubmed publisher
  36. Sivaraman J, Myers R, Boju L, Sulea T, Cygler M, Jo Davisson V, et al. Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI. Biochemistry. 2005;44:10071-80 pubmed publisher
    ..The putative substrate-binding cleft is formed at the interface between the two monomers of the dimer. This fact, combined with the localization of the Zn(2+)-binding site, indicates that the enzyme is an obligate dimer...
  37. Ellis J, Barnes J, Brown J. Is Alba an RNase P subunit?. RNA Biol. 2007;4:169-72 pubmed
    ..In addition, the presence of Mth1483p did not enhance the activity of RNase P holoenzyme reconstituted from recombinant subunits. In conclusion, we find no evidence that Alba is an RNase P subunit...
  38. Peterhoff D, Beer B, Rajendran C, Kumpula E, Kapetaniou E, Guldan H, et al. A comprehensive analysis of the geranylgeranylglyceryl phosphate synthase enzyme family identifies novel members and reveals mechanisms of substrate specificity and quaternary structure organization. Mol Microbiol. 2014;92:885-99 pubmed publisher
    ..Most of the group II enzymes form hexamers. We could disrupt these hexamers to stable and catalytically active dimers by mutating a single amino acid that acts as an 'aromatic anchor'. ..
  39. Kozlov G, Ekiel I, Beglova N, Yee A, Dharamsi A, Engel A, et al. Rapid fold and structure determination of the archaeal translation elongation factor 1beta from Methanobacterium thermoautotrophicum. J Biomol NMR. 2000;17:187-94 pubmed
    ..This novel feature was not observed previously and may serve a structural function related to protein stability or may play a functional role in archaeal protein translation. ..
  40. Keller J, Smith P, Benach J, Christendat D, DeTitta G, Hunt J. The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase. Structure. 2002;10:1475-87 pubmed
    ..Therefore, MT0146/CbiT probably functions as a precorrin methyltransferase and represents the first enzyme identified with this activity that does not have the canonical precorrin methyltransferase fold...
  41. Takao M, Yasui A, Oikawa A. Unique characteristics of superoxide dismutase of a strictly anaerobic archaebacterium Methanobacterium thermoautotrophicum. J Biol Chem. 1991;266:14151-4 pubmed
    ..Moreover, M. thermoautotrophicum SOD is resistant to azide and hydrogen peroxide as MnSODs are, suggesting that its evolution is distinct from known eubacterial FeSODs...
  42. Alex L, Reeve J, Orme Johnson W, Walsh C. Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H. Biochemistry. 1990;29:7237-44 pubmed
    ..The amino acid sequences of the subunits of the FRH are compared with those of other Ni-containing hydrogenases, including the methyl viologen reducing hydrogenase (MVH) of M. thermoautotrophicum delta H...
  43. Pihl T, Sharma S, Reeve J. Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H. J Bacteriol. 1994;176:6384-91 pubmed
  44. Leroux M, F ndrich M, Klunker D, Siegers K, Lupas A, Brown J, et al. MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldin. EMBO J. 1999;18:6730-43 pubmed publisher
    ..In light of the absence of Hsp70 chaperones in many archaea, GimC may fulfil an ATP-independent, Hsp70-like function in archaeal de novo protein folding...
  45. Ye H, Chen T, Xu X, Pennycooke M, Wu H, Steegborn C. Crystal structure of the putative adapter protein MTH1859. J Struct Biol. 2004;148:251-6 pubmed publisher
    ..These interactions lead to the formation of a honeycomb structure and suggest that the family of MTH1859-like proteins might function as adapters for protein complex assembly...
  46. N lling J, Pihl T, Reeve J. Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri. J Bacteriol. 1995;177:7238-44 pubmed
  47. Hochheimer A, Hedderich R, Thauer R. The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzyme. Arch Microbiol. 1998;170:389-93 pubmed
    ..In both organisms, the tungsten isoenzyme was found to be constitutively transcribed, whereas the transcription of the molybdenum operon was induced by molybdate. Induction by molybdate was not significantly affected by tungstate...
  48. Kelman Z, Pietrokovski S, Hurwitz J. Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH. J Biol Chem. 1999;274:28751-61 pubmed
    ..thermoautotrophicum can be relieved by the addition of M. thermoautotrophicum homologs of replication factor C and proliferating cell nuclear antigen. The possible roles of PolB in M. thermoautotrophicum replication are discussed...
  49. Christendat D, Saridakis V, Dharamsi A, Bochkarev A, Pai E, Arrowsmith C, et al. Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDP. J Biol Chem. 2000;275:24608-12 pubmed publisher
    ..The conservation of the active site residues suggests that the mechanism of action is also conserved and that the RmlC structure may be useful in guiding the design of antibacterial drugs...
  50. Mackereth C, Arrowsmith C, Edwards A, McIntosh L. Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 2000;97:6316-21 pubmed
    ..One or more of these regions may contribute to the role of this subunit as a scaffold protein within the polymerase holoenzyme...
  51. Strop P, Smith K, Iverson T, Ferry J, Rees D. Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. J Biol Chem. 2001;276:10299-305 pubmed publisher
    ..The structure reveals a Hepes buffer molecule bound 8 A away from the active site zinc, which suggests a possible proton transfer pathway from the active site to the solvent...
  52. Roll Mecak A, Cao C, Dever T, Burley S. X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding. Cell. 2000;103:781-92 pubmed
    ..Mechanisms of GTPase function and ribosome binding are discussed...
  53. Hall T, Brown J. Archaeal RNase P has multiple protein subunits homologous to eukaryotic nuclear RNase P proteins. RNA. 2002;8:296-306 pubmed
    ..RNase P in Archaea is therefore composed of an RNA subunit similar to bacterial RNase P RNA and multiple protein subunits similar to those in the eukaryotic nucleus...
  54. Daiyasu H, Hiroike T, Koga Y, Toh H. Analysis of membrane stereochemistry with homology modeling of sn-glycerol-1-phosphate dehydrogenase. Protein Eng. 2002;15:987-95 pubmed
  55. Lee J, Kozono D, Remis J, Kitagawa Y, Agre P, Stroud R. Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A. Proc Natl Acad Sci U S A. 2005;102:18932-7 pubmed publisher
    ..As a result of this and other side-chain substituents in the walls of the channel, the channel is intermediate in size and exhibits differentially tuned electrostatics when compared with the other subfamilies...
  56. Zirngibl C, Van Dongen W, Schw rer B, Von B nau R, Richter M, Klein A, et al. H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea. Eur J Biochem. 1992;208:511-20 pubmed
    ..thermoautotrophicum with respect both to molecular and catalytic properties...
  57. N lling J, Pihl T, Vriesema A, Reeve J. Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome. J Bacteriol. 1995;177:2460-8 pubmed
    ..thermoautotrophicum deltaH, was found to occur at all growth stages...
  58. Mukhopadhyay B, Stoddard S, Wolfe R. Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaH. J Biol Chem. 1998;273:5155-66 pubmed
    ..thermoautotrophicum strain DeltaH (Kenealy, W. R., and Zeikus, J. G. (1982) FEMS Microbiol. Lett. 14, 7-10) raise several questions for future investigations...
  59. Christendat D, Yee A, Dharamsi A, Kluger Y, Savchenko A, Cort J, et al. Structural proteomics of an archaeon. Nat Struct Biol. 2000;7:903-9 pubmed publisher
    ..This demonstrates that structural proteomics is feasible and can play a central role in functional genomics...
  60. Batra R, Christendat D, Edwards A, Arrowsmith C, Tong L. Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase. Proteins. 2002;49:285-8 pubmed publisher
  61. Boomershine W, McElroy C, Tsai H, Wilson R, Gopalan V, Foster M. Structure of Mth11/Mth Rpp29, an essential protein subunit of archaeal and eukaryotic RNase P. Proc Natl Acad Sci U S A. 2003;100:15398-403 pubmed publisher
    ..Our findings represent a critical step toward a structural model of the RNase P holoenzyme from archaebacteria and higher organisms...
  62. Guy C, Bolt E. Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands. Nucleic Acids Res. 2005;33:3678-90 pubmed publisher
    ..We speculate that removal of lagging strands at stalled forks by Hel308 promotes the formation of initiation zones, priming restart of lagging strand synthesis...
  63. Bello A, Poduch E, Fujihashi M, Amani M, Li Y, Crandall I, et al. A potent, covalent inhibitor of orotidine 5'-monophosphate decarboxylase with antimalarial activity. J Med Chem. 2007;50:915-21 pubmed publisher
    ..2 +/- 0.7 microM against P. falciparum ItG and 3D7 isolates, respectively. 6-Iodouridine 5'-monophosphate is a novel covalent inhibitor of ODCase, and its nucleoside analogue paves the way to a new class of inhibitors against malaria...
  64. Woodman I, Briggs G, Bolt E. Archaeal Hel308 domain V couples DNA binding to ATP hydrolysis and positions DNA for unwinding over the helicase ratchet. J Mol Biol. 2007;374:1139-44 pubmed publisher
    ..We describe crucial roles for domain V as a brake on ATP hydrolysis by coupling it to binding single-stranded DNA and in positioning DNA relative to the helicase ratchet domain IV for efficient unwinding of forked DNA...
  65. Silva A, Chechik M, Byrne R, Waterman D, Ng C, Dodson E, et al. Structure and activity of a novel archaeal ?-CASP protein with N-terminal KH domains. Structure. 2011;19:622-32 pubmed publisher
    ..We hypothesize that this nuclease degrades mRNAs of proteins targeted for degradation and so regulates archaeal RNA turnover, possibly in concert with the exosome...
  66. Rospert S, Linder D, Ellermann J, Thauer R. Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H. Eur J Biochem. 1990;194:871-7 pubmed
    ..When the cultures were supplied with sufficient H2 and and CO2 and the cells grew exponentially, essentially only MCR II was found. When growth was limited by the gas supply, MCR I predominated...
  67. Ma K, Thauer R. Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1990;191:187-93 pubmed
    ..3 mM and 3 microM, respectively. Vmax was 6000 mumol.min-1.mg protein-1 (kcat = 3600 s-1). The CH2 = H4MPT reductase was stable in the presence of air; at 4 C less than 10% activity was lost within 24 h...
  68. N lling J, Ishii M, Koch J, Pihl T, Reeve J, Thauer R, et al. Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum. Eur J Biochem. 1995;231:628-38 pubmed
    ..abstract truncated at 400 words)..
  69. Hedderich R, Koch J, Linder D, Thauer R. The heterodisulfide reductase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic of pyridine-nucleotide-dependent thioredoxin reductases. Eur J Biochem. 1994;225:253-61 pubmed
    ..46 kDa) did not show sequence similarity to other known proteins, but appears to possess a C-terminal hydrophobic alpha-helix that might function as a membrane anchor. Although hdrB and hdrC are juxtaposed, these genes are not near hdrA...
  70. Setzke E, Hedderich R, Heiden S, Thauer R. H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties. Eur J Biochem. 1994;220:139-48 pubmed
    ..The stimulatory effects of potassium phosphate, a membrane component, uracil derivatives and coenzyme F430 on the H2:heterodisulfide-oxidoreductase activity of the purified complex are described...
  71. G rtner P, Ecker A, Fischer R, Linder D, Fuchs G, Thauer R. Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1993;213:537-45 pubmed
    ..The apparent Km for N5-methyltetrahydromethanopterin was 260 microM and that for coenzyme M was 60 microM. The preparation was absolutely dependent on the presence of Ti(III) for activity. ATP enhanced the activity 1.5-2-fold...
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