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Genomes and Genes | Methanothermobacter thermautotrophicus str. Delta HSummaryAlias: Methanobacterium thermoautotrophicum str. Delta H, Methanothermobacter thermautotrophicus str. deltaH, Methanobacterium thermoautotrophicum str. deltaH Top Publications
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An examination of the relationship between active site loop size and thermodynamic activation parameters for orotidine 5'-monophosphate decarboxylase from mesophilic and thermophilic organismsKrisztina Toth
Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260 3000, USA
Biochemistry 48:8006-13. 2009....- The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetasesSatwik Kamtekar
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
Proc Natl Acad Sci U S A 100:1673-8. 2003..jannaschii ProRS misacylates tRNA(Pro) with cysteine, and argue against the proposal that these archaeal ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cognate amino acids... - Deep trefoil knot implicated in RNA binding found in an archaebacterial proteinThomas I Zarembinski
Biosciences Division, Structural Biology Center, Argonne National Laboratory, Argonne, Illinois 60439, USA
Proteins 50:177-83. 2003 
The open pore conformation of potassium channelsYouxing Jiang
Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
Nature 417:523-6. 2002..The open conformation favours high conduction by compressing the membrane field to the selectivity filter, and also permits large organic cations and inactivation peptides to enter the pore from the intracellular solution...- Crystal structure and mechanism of a calcium-gated potassium channelYouxing Jiang
Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
Nature 417:515-22. 2002..Eight RCK domains (regulators of K(+) conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca(2+) binding in a simple manner to perform mechanical work to open the pore... - Identification of a novel archaebacterial thioredoxin: determination of function through structureSudeepa Bhattacharyya
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
Biochemistry 41:4760-70. 2002..On the basis of the results presented here, we predict that these small proteins are all members of a new class of truncated thioredoxins... - Mapping the active site-ligand interactions of orotidine 5'-monophosphate decarboxylase by crystallographyNing Wu
Department of Biochemistry, Centres of Excellence, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8, Canada
Biochemistry 41:4002-11. 2002..Consequently, orotidine 5'-monophosphate decarboxylases seem to require the presence of a negative charge at this position for catalysis as well as for correct and stable folding... - On the mechanism of biological methane formation: structural evidence for conformational changes in methyl-coenzyme M reductase upon substrate bindingW Grabarse
Max Planck Institut fur Biophysik, Frankfurt, Germany
J Mol Biol 309:315-30. 2001..The three different enzymatically inactive enzyme states are discussed with respect to their enzymatically active precursors and with respect to the catalytic mechanism... - The crystal structure of a heptameric archaeal Sm protein: Implications for the eukaryotic snRNP coreC Mura
University of California at Los Angeles Department of Energy Laboratory of Structural Biology and Molecular Medicine, 201 Boyer Hall Molecular Biology Institute, Box 951570, Los Angeles, CA 90095 1570, USA
Proc Natl Acad Sci U S A 98:5532-7. 2001..The pronounced electrostatic asymmetry of the SmAP surface imparts directionality to putative SmAP-RNA interactions... - Structural proteomics of an archaeonD Christendat
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9
Nat Struct Biol 7:903-9. 2000..This demonstrates that structural proteomics is feasible and can play a central role in functional genomics... - Domain-specific recruitment of amide amino acids for protein synthesisD L Tumbula
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
Nature 407:106-10. 2000..Closer inspection of the two amidotransferases reveals that each of them recruited a metabolic enzyme to aid its function; this provides direct evidence for a relationship between amino-acid metabolism and protein biosynthesis... - Rapid fold and structure determination of the archaeal translation elongation factor 1beta from Methanobacterium thermoautotrophicumG Kozlov
McGill University, Department of Biochemistry, Montreal, PQ, Canada
J Biomol NMR 17:187-94. 2000..This novel feature was not observed previously and may serve a structural function related to protein stability or may play a functional role in archaeal protein translation... - Purification, crystallization and preliminary X-ray study of orotidine 5'-monophosphate decarboxylaseN Wu
Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario, M5S 1A8, Canada
Acta Crystallogr D Biol Crystallogr 56:912-4. 2000..0, b = 98.6, c = 73.3 A, gamma = 104.0 degrees ), with four molecules in the asymmetric unit; the crystals diffract to 1.5 A resolution... - Structure of coenzyme F(420) dependent methylenetetrahydromethanopterin reductase from two methanogenic archaeaS Shima
Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Strasse, Marburg, 35043, Germany
J Mol Biol 300:935-50. 2000..However, Mer and MetF do not show sequence similarities although they bind related substrates and catalyze an analogous reaction... - Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicumA Yee
Division of Molecular and Structural Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 2M9, Canada
Proc Natl Acad Sci U S A 97:6311-5. 2000..The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein... - Electrostatic stress in catalysis: structure and mechanism of the enzyme orotidine monophosphate decarboxylaseN Wu
Department of Biochemistry, Centres of Excellence, University of Toronto, 1 King s College Circle, Toronto, ON Canada M5S 1A8
Proc Natl Acad Sci U S A 97:2017-22. 2000..The computational results are consistent with a catalytic mechanism that is characterized by Jencks's Circe effect... - The biosynthesis of methylated amino acids in the active site region of methyl-coenzyme M reductaseT Selmer
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
J Biol Chem 275:3755-60. 2000..A mechanism for the methylation of glutamine at C-2 and of arginine at C-5 is discussed... - Phenylalanyl-tRNA synthetase from the archaeon Methanobacterium thermoautotrophicum is an (alphabeta)2 heterotetrameric proteinR Das
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114 USA
Biochimie 81:1037-9. 1999..Two ORFs with similarity to non-archaeal PheRSs alpha-subunits had previously been found in the genome sequence, but these results show that only one of them, MT742, is part of the active PheRS... - Analysis of membrane stereochemistry with homology modeling of sn-glycerol-1-phosphate dehydrogenaseHiromi Daiyasu
Department of Computational Biology, Biomolecular Engineering Research Institute, 6 2 3, Furuedai, Suita, Osaka 565 0874, Japan
Protein Eng 15:987-95. 2002.... - A story of chelatase evolution: identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaeaAmanda A Brindley
School of Biological Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom
J Biol Chem 278:22388-95. 2003..A classification scheme for chelatases is proposed... - Conformational flexibility and molecular interactions of an archaeal homologue of the Shwachman-Bodian-Diamond syndrome proteinC Leong Ng
York Structural Biology Laboratory, Chemistry Department, University of York, York, YO10 5YW, UK
BMC Struct Biol 9:32. 2009..Although genomic and biophysical studies have suggested involvement of this protein in RNA metabolism and in ribosome biogenesis, its interacting partners remain largely unknown... - Specific DNA binding of a potential transcriptional regulator, inosine 5'-monophosphate dehydrogenase-related protein VII, to the promoter region of a methyl coenzyme m reductase I-encoding operon retrieved from Methanothermobacter thermautotrophicus straNaoya Shinzato
Center of Molecular Biosciences, University of the Ryukyus, Nishihara cho, Okinawa 903 0213, Japan
Appl Environ Microbiol 74:6239-47. 2008..The results presented here suggest that IMPDH VII encoded by MTH126 is a plausible candidate for the transcriptional regulator of the mcr operon in this methanogen... - Methanogens with pseudomurein use diaminopimelate aminotransferase in lysine biosynthesisDavid E Graham
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712 0165, USA
FEBS Lett 582:1369-74. 2008..In contrast marine methanogens from the Methanococcales, which lack pseudomurein, appear to use a different diaminopimelate pathway for lysine biosynthesis... - Crystal structures of a ligand-free MthK gating ring: insights into the ligand gating mechanism of K+ channelsSheng Ye
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Cell 126:1161-73. 2006..Our findings, along with the previously determined open MthK structure, allow us to elucidate the ligand gating mechanism of RCK-regulated K(+) channels... - MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like repeatsOlivier Julien
Département de biochimie et de microbiologie and CREFSIP, Universite Laval, 3255 pav Marchand, Quebec, G1K 7P4, Canada
J Biomol NMR 35:149-54. 2006..Harrison, D., Hoang, L., Keagle, P., Lumm, W., Pothier, B., Qiu, D., Spadafora, R., Vicaire, R., Wang, Y., Wierzbowski, J., Gibson, R., Jiwani, N., Caruso, A., Bush, D., Reeve, J. N. et al. (1997) J. Bacteriol., 179, 7135-7155]... - Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 AJohn K Lee
Macromolecular Structure Group, Department of Biochemistry and Biophysics, University of California, S 412C Genentech Hall, 600 16th Street, San Francisco, CA 94143 2240, USA
Proc Natl Acad Sci U S A 102:18932-7. 2005..As a result of this and other side-chain substituents in the walls of the channel, the channel is intermediate in size and exhibits differentially tuned electrostatics when compared with the other subfamilies... - Structures of the MthK RCK domain and the effect of Ca2+ on gating ring stabilityJianbo Dong
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
J Biol Chem 280:41716-24. 2005..Additionally, our stability studies indicated that Ca2+ binding stabilizes the RCK domains in this octameric state... - Solution Structure of MTH0776 from Methanobacterium thermoautotrophicumGodwin Amegbey
Department of Computing Science and Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, T6G 2E8
J Biomol NMR 33:51-6. 2005 - Structural polymorphism of Methanothermobacter thermautotrophicus MCMYen Ju Chen
Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
J Mol Biol 346:389-94. 2005..This offers new insight into the conformational dynamics of MCM and the phosphorylation-bypass phenotype in yeast... - The crystal structure of archaeal nascent polypeptide-associated complex (NAC) reveals a unique fold and the presence of a ubiquitin-associated domainThomas Spreter
Institute for Chemistry Crystallography, Free University of Berlin, Takustrasse 6, D 14195 Berlin, Germany
J Biol Chem 280:15849-54. 2005..Based on the presented structure we propose a model for the eukaryotic heterodimeric NAC domain... - Solution structure of the hypothetical protein Mth677 from Methanobacterium thermoautotrophicum: a novel alpha+beta foldFrancisco J Blanco
NMR Group, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
Protein Sci 13:1458-65. 2004..coli... - Solution structure of a novel calcium binding protein, MTH1880, from Methanobacterium thermoautotrophicumChang Hun Lee
Department of Biochemistry, College of Science, Yonsei University, 134 Seodaemoon Gu, Shinchondong, Seoul, Korea 120 749
Protein Sci 13:1148-54. 2004..Therefore, we propose that the MTH1880 protein contains a novel motif for calcium-specific binding, and may function as a calcium buffering protein... - Transfer of pro-R hydrogen from NADH to dihydroxyacetonephosphate by sn-glycerol-1-phosphate dehydrogenase from the archaeon Methanothermobacter thermautotrophicusYosuke Koga
Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu, Japan
Biosci Biotechnol Biochem 67:1605-8. 2003..These results confirmed a prediction of the tertiary structure of sn-glycerol-1-phosphate dehydrogenase by homology modeling... - Crystal structures of MTH1187 and its yeast ortholog YBL001cXiao Tao
Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, New York 10027, USA
Proteins 52:478-80. 2003 - Physiological role of the F420-non-reducing hydrogenase (Mvh) from Methanothermobacter marburgensisAlexander Stojanowic
Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Strasse, 35043, Marburg, Germany
Arch Microbiol 180:194-203. 2003.... - Structural and functional characterization of a thioredoxin-like protein (Mt0807) from Methanobacterium thermoautotrophicumGodwin Y Amegbey
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada T6G 2N8
Biochemistry 42:8001-10. 2003..thermoautotrophicum, while Mt0895 plays a minor or supportive role. We also suggest that these two molecules (Mt0807 and Mt0895) may represent a group of ancient proteins that were ancestral to both thioredoxins and glutaredoxins... - Mutational, structural, and kinetic studies of the ATP-binding site of Methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferaseVivian Saridakis
Molecular and Structural Biology, Ontario Cancer Institute, University Health Network, and the Department of Medical Biophysics, University of Toronto, Ontario, Canada
J Biol Chem 278:34356-63. 2003..3%, respectively, of WT NMNATase activity indicating that His-19 is a key catalytic group. Surprisingly, this H19A mutant displayed a novel and distinct mode of NAD+ binding when co-crystallized in the presence of NAD+ and SO42-... - Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteinsA Tersteegen
Max Planck Institut für Mikrobiologie, Marburg, Germany
Eur J Biochem 264:930-43. 1999..A function of these putative membrane-bound [NiFe] hydrogenases as proton pumps involved in endergonic reactions, such as the synthesis of formylmethanofuran from CO2, H2 and methanofuran, is discussed... - The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrHB Hippler
Max Planck Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps Universität, Marburg, Germany
FEBS Lett 449:165-8. 1999..Sequence comparison revealed similarity of MtrH with MetH from Escherichia coli and AcsE from Clostridium thermoaceticum: both enzymes exhibit methyltetrahydrofolate:cob(I)alamin methyltransferase activity... - The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotideA Hochheimer
Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat, Marburg, Germany
Eur J Biochem 234:910-20. 1995..The fwd operon was found to be located in a region of the M. thermoautotrophicum genome encoding molybdenum enzymes and proteins involved in molybdopterin biosynthesis... - Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acidU Jenal
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
J Biol Chem 266:10570-7. 1991..Both the mutant and the wild-type ileS gene were expressed in E. coli, and their products displayed the expected difference in sensitivity toward pseudomonic acid... 
Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNAKristina Lakomek
Department of Molecular Structural Biology, Institute of Microbiology and Genetics, Georg August University Gottingen, Justus von Liebig Weg 11, D 37077 Gottingen, Germany
J Mol Biol 399:604-17. 2010..In addition, Ser171, Asn153, and Lys125 in the substrate binding pocket appear to have important functions in the discrimination of aberrant uridine against naturally occurring thymidine and cytosine residues in double-stranded DNA...- Transcription of the ileS operon in the archaeon Methanobacterium thermoautotrophicum MarburgU Jenal
Mikrobiologisches Institut, Swiss Federal Institute of Technology, ETH Zentrum, Zurich
J Bacteriol 175:5945-52. 1993..Extensive decay of the orf401-ileS-purL message was observed. Degradation occurred, presumably by endonucleolytic cleavage, within the orf401 region... - Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coliB Mukhopadhyay
Department of Microbiology, University of Iowa, Iowa City 52242
J Biol Chem 270:2827-32. 1995..The mtd coding sequence was followed by several poly(dT) sequences and an inverted repeat that could be transcription termination signals... - Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta HS Rospert
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat Marburg, Federal Republic of Germany
Eur J Biochem 194:871-7. 1990..When the cultures were supplied with sufficient H2 and and CO2 and the cells grew exponentially, essentially only MCR II was found. When growth was limited by the gas supply, MCR I predominated... - Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta HT D Pihl
Department of Microbiology, Ohio State University, Columbus 43210
J Bacteriol 176:6384-91. 1994.... - The heterodisulfide reductase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic of pyridine-nucleotide-dependent thioredoxin reductasesR Hedderich
Max Planck Institut für terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie, Philipps Universitat Marburg, Germany
Eur J Biochem 225:253-61. 1994..46 kDa) did not show sequence similarity to other known proteins, but appears to possess a C-terminal hydrophobic alpha-helix that might function as a membrane anchor. Although hdrB and hdrC are juxtaposed, these genes are not near hdrA... - The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunitsU Harms
Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat, Marburg, Germany
Eur J Biochem 228:640-8. 1995..The mtr operon was found to be located between the methyl-coenzyme M reductase I operon (mcr) and a downstream open reading frame predicted to encode a Na+/Ca2+, K+ exchanger... - Sequence homologies in the N-terminal region of the ribosomal 'A' proteins from Methanobacterium Thermoautotrophicum and Halobacterium cutirubrumA T Matheson
Biochim Biophys Acta 626:162-9. 1980..The sequence data from the 'A' proteins also indicate that, phylogenetically, the archaebacteria are much closer to the cytoplasmic components of eucaryotes than they are to the eubacteria (or 'true bacteria')... - A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxinJ N Reeve
Department of Microbiology, Ohio State University, Columbus 43210
Proc Natl Acad Sci U S A 86:3031-5. 1989..The product of the mvhB gene is predicted to contain six tandomly repeated bacterial-ferredoxin-like domains and, therefore, is predicted to be a polyferredoxin that could contain as many as 48 iron atoms in 12 Fe4S4 clusters... - Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalentsB Berghöfer
Department of Biology, Philipps University, Marburg, FRG
Nucleic Acids Res 16:8113-28. 1988..Putative functional domains were identified in two of the subunits of the archaebacterial enzyme... - Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleriJ NOLLING
Department of Microbiology, Ohio State University, Columbus 43210, USA
J Bacteriol 177:7238-44. 1995.... - Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systemsK Kleman-Leyer
Department of Microbiology, The Ohio State University, Columbus 43210, USA
Cell 89:839-47. 1997..These results provide evidence that the archaeal and eucaryal tRNA intron processing systems are related and suggest a common origin for tRNA introns in these organisms... - H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaeaC Zirngibl
Laboratorium für Mikrobiologie des Fachbereichs Biologie, Philipps Universitat Marburg, Federal Republic of Germany
Eur J Biochem 208:511-20. 1992..thermoautotrophicum with respect both to molecular and catalytic properties... - The molybdenum formylmethanofuran dehydrogenase operon and the tungsten formylmethanofuran dehydrogenase operon from Methanobacterium thermoautotrophicum. Structures and transcriptional regulationA Hochheimer
Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat Marburg, Germany
Eur J Biochem 242:156-62. 1996..thermo-autotrophicum revealed that the fwdHFGDACB gene cluster is transcribed in the presence of either molybdate or tungstate in the growth medium whereas the fmdECB gene cluster was only transcribed when molybdate was present... - Crystal structure of methyl-coenzyme M reductase: the key enzyme of biological methane formationU Ermler
Max Planck Institut fur Biophysik, Heinrich Hoffmann Strabetae 7, 60528 Frankfurt, Germany
Science 278:1457-62. 1997..Together with a second structurally characterized enzyme state (MCRsilent) containing the heterodisulfide of coenzymes M and B, a reaction mechanism is proposed that uses a radical intermediate and a nickel organic compound... - Purification, regulation, and molecular and biochemical characterization of pyruvate carboxylase from Methanobacterium thermoautotrophicum strain deltaHB Mukhopadhyay
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
J Biol Chem 273:5155-66. 1998..thermoautotrophicum strain DeltaH (Kenealy, W. R., and Zeikus, J. G. (1982) FEMS Microbiol. Lett. 14, 7-10) raise several questions for future investigations... - The hemA gene encoding glutamyl-tRNA reductase from the archaeon Methanobacterium thermoautotrophicum strain MarburgC Hungerer
Laboratorium für Mikrobiologie des Fachbereich Biologie der Philipps Universität, Marburg, Germany
Bioorg Med Chem 4:1089-95. 1996..0. The reductase utilized preferentially NADPH for the reduction of the activated carboxyl group. The presence of ATP and GTP showed no obvious influence on catalysis... - Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coliM Vaupel
Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
Eur J Biochem 236:294-300. 1996..The mch gene was overexpressed in Escherichia coli yielding an active enzyme of 37 kDa with a specific activity of 30 U/mg cell extract protein... - A family of flavoproteins in the domains Archaea and BacteriaA Wasserfallen
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
Eur J Biochem 254:325-32. 1998..gigas hemoflavoprotein [Gomes, C. M., Silva, G., Oliveira, S., LeGall, J., Liu, M.-Y., Xavier, A. V., Rodrigues-Pousada, C. & Teixeira, M. (1997) J. Biol. Chem. 272, 22502-22508], is discussed... - The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzymeA Hochheimer
Max Planck Institut fur terrestrische Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
Arch Microbiol 170:389-93. 1998..In both organisms, the tungsten isoenzyme was found to be constitutively transcribed, whereas the transcription of the molybdenum operon was induced by molybdate. Induction by molybdate was not significantly affected by tungstate... - Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operonL Meile
Mikrobiologisches Institut, Swiss Federal Institute of Technology, Zurich, Switzerland
J Bacteriol 173:5017-23. 1991..With the exception of TrpB, the beta subunit of tryptophan synthase, tryptophan was absent from all Trp polypeptides... - RNA-dependent cysteine biosynthesis in archaeaAnselm Sauerwald
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
Science 307:1969-72. 2005.... - An unprecedented twist to ODCase catalytic activityMasahiro Fujihashi
Molecular Design and Information Technology Center, Leslie Dan Faculty of Pharmacy, Toronto, ON M5S 2S2 Canada
J Am Chem Soc 127:15048-50. 2005..This potential of ODCase is very useful in the design of novel inhibitors... - Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg)K Ma
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Federal Republic of Germany
Eur J Biochem 191:187-93. 1990..3 mM and 3 microM, respectively. Vmax was 6000 mumol.min-1.mg protein-1 (kcat = 3600 s-1). The CH2 = H4MPT reductase was stable in the presence of air; at 4 C less than 10% activity was lost within 24 h... - A recombinant exonuclease III homologue of the thermophilic archaeon Methanothermobacter thermautotrophicusSven Pfeifer
Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig Biotechnological Biomedical Centre Leipzig, Bruderstrasse 34, 04103 Leipzig, Germany
DNA Repair (Amst) 4:433-44. 2005..Considering the high intracellular potassium ion concentration in M. thermautotrophicus, our results suggest that the characterized thermophilic enzyme acts as an AP endonuclease in vivo with similar activities as Ape1... - Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta HB W Te Brommelstroet
Department of Microbiology, University of Nijmegen, The Netherlands
J Biol Chem 265:1852-7. 1990.... - Unique characteristics of superoxide dismutase of a strictly anaerobic archaebacterium Methanobacterium thermoautotrophicumM Takao
Department of Biosciences, School of Hygienic Sciences, Kitasato University, Sagamihara, Japan
J Biol Chem 266:14151-4. 1991..Moreover, M. thermoautotrophicum SOD is resistant to azide and hydrogen peroxide as MnSODs are, suggesting that its evolution is distinct from known eubacterial FeSODs... - 8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterizationJ A Fox
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139
Biochemistry 26:4219-27. 1987..There is iron associated with the alpha-subunit, but placement of the nickel and FAD has not been established... - Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta HL A Alex
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Biochemistry 29:7237-44. 1990..The amino acid sequences of the subunits of the FRH are compared with those of other Ni-containing hydrogenases, including the methyl viologen reducing hydrogenase (MVH) of M. thermoautotrophicum delta H... - Characterization of a superoxide dismutase gene from the archaebacterium Methanobacterium thermoautotrophicumM Takao
Department of Bioscience, School of Hygienic Science, Kitasato University, Sagamihara, Japan
Arch Biochem Biophys 283:210-6. 1990..Between a putative promoter and the start codon there is an inverted repeat sequence which is also found in the counterpart of Halobacterium halobium... - A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolaseYou Na Kang
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
Biochemistry 46:5050-62. 2007.... - Is Alba an RNase P subunit?J Chris Ellis
Laboratory of Signal Transduction, NIEHS, Research Triangle Park, North Carolina, USA
RNA Biol 4:169-72. 2007..In addition, the presence of Mth1483p did not enhance the activity of RNase P holoenzyme reconstituted from recombinant subunits. In conclusion, we find no evidence that Alba is an RNase P subunit... - A potent, covalent inhibitor of orotidine 5'-monophosphate decarboxylase with antimalarial activityAngelica M Bello
Center for Molecular Design and Preformulations, Toronto General Research Institute, Toronto General Hospital, Toronto, ON M5G 2C4 Canada
J Med Chem 50:915-21. 2007..2 +/- 0.7 microM against P. falciparum ItG and 3D7 isolates, respectively. 6-Iodouridine 5'-monophosphate is a novel covalent inhibitor of ODCase, and its nucleoside analogue paves the way to a new class of inhibitors against malaria... - Sequence divergence of an archaebacterial gene cloned from a mesophilic and a thermophilic methanogenP T Hamilton
J Mol Evol 22:351-60. 1985..Although the two purE-complementing genes are apparently derived from a common ancestor, only the gene from M. smithii maintains a codon usage that conforms to the RNY rule... - Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245J NOLLING
Department of Microbiology, The Ohio State University, Columbus 43210, USA
J Bacteriol 179:899-908. 1997..Sequencing revealed the presence of very similar genes except that the genome of M. thermoautotrophicum, a methanogen incapable of growth on formate, lacked the fdhCAB operon... - Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicumP Strop
Biochemistry Option, California Institute of Technology, Pasadena, California 91125, USA
J Biol Chem 276:10299-305. 2001..The structure reveals a Hepes buffer molecule bound 8 A away from the active site zinc, which suggests a possible proton transfer pathway from the active site to the solvent... - Sequence divergence of seryl-tRNA synthetases in archaeaH S Kim
Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8114, USA
J Bacteriol 180:6446-9. 1998..Gel shift experiments showed that M. thermoautotrophicum SerRS did not mischarge tRNACys with serine. This indicates that Cys-tRNACys is formed by direct acylation in these organisms... - Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicumP Gartner
Laboratorium für Mikrobiologie des Fachbereichs Biologie, Philipps Universitat Marburg, Germany
Eur J Biochem 213:537-45. 1993..The apparent Km for N5-methyltetrahydromethanopterin was 260 microM and that for coenzyme M was 60 microM. The preparation was absolutely dependent on the presence of Ti(III) for activity. ATP enhanced the activity 1.5-2-fold... - Methyl viologen hydrogenase II, a new member of the hydrogenase family from Methanobacterium thermoautotrophicum delta HG J Woo
Department of Microbiology, University of Illinois, Urbana 61801
J Bacteriol 175:5970-7. 1993..Both hydrogenases were shown to be stable for over 8 days at --20 degrees C under anaerobic conditions. When exposed to air, 90% of MVH I activity was lost within 2 min; however, MVH II lost only 50% of its activity in 3 h... - Archaeal RNase P has multiple protein subunits homologous to eukaryotic nuclear RNase P proteinsThomas A Hall
Department of Microbiology, North Carolina State University, Raleigh 27695 7615, USA
RNA 8:296-306. 2002..RNase P in Archaea is therefore composed of an RNA subunit similar to bacterial RNase P RNA and multiple protein subunits similar to those in the eukaryotic nucleus... - Crystal structure of Methanobacterium thermoautotrophicum conserved protein MTH1020 reveals an NTN-hydrolase foldVivian Saridakis
Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Ontario, Canada
Proteins 48:141-3. 2002 - Cloning, sequencing and immunological characterization of the corrinoid-containing subunit of the N5-methyltetrahydromethanopterin: coenzyme-M methyltransferase from Methanobacterium thermoautotrophicumE Stupperich
Institut fur Mikrobiologie, Gottingen, Germany
Eur J Biochem 217:115-21. 1993..The N-terminal sequences of mtrC and mtrD are identical with two peptides of the N5-methyltetrahydromethanopterin:coenzyme-M methyltransferase complex from Methanobacterium, indicating that the mtr genes encode this membrane protein... - Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetasesIvan Ahel
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
J Biol Chem 277:34743-8. 2002..Therefore, ProRS exhibits a natural level of mischarging that is to date unequalled among the aminoacyl-tRNA synthetases... - Aspartate aminotransferase from a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase familyT Tanaka
Department of Biology, Faculty of Science, Osaka City University
J Biochem 115:309-17. 1994..K., Hale, T.I., & Christen, P. (1993) Eur. J. Biochem. 214, 549-561], but not to subgroup I, in which all the AspATs known so far are included... - H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and propertiesE Setzke
Max Planck Institut für Terrestrsche Mikrobiologie, Marburg, Germany
Eur J Biochem 220:139-48. 1994..The stimulatory effects of potassium phosphate, a membrane component, uracil derivatives and coenzyme F430 on the H2:heterodisulfide-oxidoreductase activity of the purified complex are described... - The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs)Cameron Mura
Howard Hughes Medical Institute, Molecular Biology Institute, Los Angeles, California 90095 1570, USA
Protein Sci 12:832-47. 2003..Finally, we found that both Pae and Mth SmAP1 gel-shift negatively supercoiled DNA. These results distinguish SmAPs from eukaryotic Sm proteins and suggest that SmAPs have a generic single-stranded nucleic acid-binding activity... - The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferaseJacob P Keller
Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA
Structure 10:1475-87. 2002..Therefore, MT0146/CbiT probably functions as a precorrin methyltransferase and represents the first enzyme identified with this activity that does not have the canonical precorrin methyltransferase fold... - Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase (Mer) from Methanobacterium thermoautotrophicum strain Marburg. Cloning, sequencing, transcriptional analysis, and functional expression in Escherichia coli of the mer geneM Vaupel
Max Planck Institut fur terrestrische Mikrobiologie, Philipps Universitat Marburg, Germany
Eur J Biochem 231:773-8. 1995..thermoautotrophicum. Sequence comparisons revealed similarities between the F420-dependent N5,N10-methylenetetrahydromethanopterin reductase and a F420-dependent reductase involved in lincomycin biosynthesis in Streptomyces lincolnensis... - Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicumJ NOLLING
Department of Microbiology, Ohio State University, Columbus, USA
Eur J Biochem 231:628-38. 1995..abstract truncated at 400 words).. - Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicumBin Wu
Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
Protein Sci 12:2831-7. 2003..A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA... - Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiationPablo Gutierrez
McGill University, Department of Biochemistry, McIntyre Medical Science Building, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada
Protein Sci 13:659-67. 2004..Based on structural similarity and biochemical data, a role for the different secondary structure elements is suggested... - A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaHZ Kelman
Department of Molecular Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
J Biol Chem 275:7327-36. 2000..The importance and roles of RFC and PCNA in M. thermoautotrophicum DeltaH replication are discussed...
Research Grants
- Biochemistry of Class I lysyl-tRNA synthetasesMichael Ibba; Fiscal Year: 2003..This, in turn, will provide a framework for developing therapeutics specifically targeted against the class I lysyl-tRNA synthetase. ..
- Archaeal gene recruitment by the ChlamydialesDavid Graham; Fiscal Year: 2007..Results from these experiments will support future microbiological studies of the role of this system in chlamydial infection and survival in the host cell. ..
- Biochemistry of Class I lysyl-tRNA synthetasesMichael Ibba; Fiscal Year: 2007..This, in turn, will provide a framework for developing therapeutics specifically targeted against the class I lysyl-tRNA synthetase. ..
- Biochemistry of Class I lysyl-tRNA synthetasesMichael Ibba; Fiscal Year: 2006..This, in turn, will provide a framework for developing therapeutics specifically targeted against the class I lysyl-tRNA synthetase. ..
- Biochemistry of lysyl-tRNA synthetasesMichael Ibba; Fiscal Year: 2010..The study will also provide further insights into the degree of functional diversity within the aaRS family in general, an emerging group of anti-microbial drug targets. ..