Methanothermobacter marburgensis str. Marburg


Alias: Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133), Methanobacterium thermoautotrophicum str. Marburg, Methanothermobacter marburgensis strain Marburg

Top Publications

  1. Tamura H, Salomone Stagni M, Fujishiro T, Warkentin E, Meyer Klaucke W, Ermler U, et al. Crystal structures of [Fe]-hydrogenase in complex with inhibitory isocyanides: implications for the H2-activation site. Angew Chem Int Ed Engl. 2013;52:9656-9 pubmed publisher
  2. Vitt S, Ma K, Warkentin E, Moll J, Pierik A, Shima S, et al. The F???-reducing [NiFe]-hydrogenase complex from Methanothermobacter marburgensis, the first X-ray structure of a group 3 family member. J Mol Biol. 2014;426:2813-26 pubmed publisher
    ..The protein might contribute to balanced redox potentials by the aspartate coordination of the proximal [4Fe-4S] cluster, the new ferredoxin module and a rather negatively charged FAD surrounding. ..
  3. Mukhopadhyay B, Purwantini E, Pihl T, Reeve J, Daniels L. Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli. J Biol Chem. 1995;270:2827-32 pubmed
    ..The mtd coding sequence was followed by several poly(dT) sequences and an inverted repeat that could be transcription termination signals. ..
  4. Harms U, Thauer R. Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum. Eur J Biochem. 1997;250:783-8 pubmed
    ..For comparison, the DNA sequences of the mtrEDCBAGH operon in M. kandleri and of the mtxXAH operon in M. barkeri were determined. ..
  5. Moore J, Chen A, Yan M, Hurlburt A, Poulter C. Identification of the gltX gene encoding glutamyl-tRNA synthetase from Methanobacterium thermoautotrophicum. Biochim Biophys Acta. 1996;1305:113-6 pubmed
    ..The deduced protein sequence from gltX contains conserved regions (HIGH and KMSKS) indicative of a class I aminoacyl-tRNA synthetase. ..
  6. Schröder I, Thauer R. Methylcobalamin:homocysteine methyltransferase from Methanobacterium thermoautotrophicum. Identification as the metE gene product. Eur J Biochem. 1999;263:789-96 pubmed
    ..1 mM). The enzyme was specific for L-homocysteine as methyl acceptor. Methylcobalamin could be substituted with methylcobinamide as methyl donor. ..
  7. Krone U, McFarlan S, Hogenkamp H. Purification and partial characterization of a putative thymidylate synthase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1994;220:789-94 pubmed
    ..However, there is no similarity with any of the thymidylate synthases. Surprisingly, the protein from M. thermoautotrophicum appears to be related to chitin synthases from several organisms. ..
  8. Meile L, Fischer K, Leisinger T. Characterization of the superoxide dismutase gene and its upstream region from Methanobacterium thermoautotrophicum Marburg. FEMS Microbiol Lett. 1995;128:247-53 pubmed
    ..The amino acid sequence for this presumptive product had a similarity coefficient of 55.5% to a subunit of the alkyl hydroperoxide reductase (encoded by the ahpC gene) from Salmonella typhimurium. ..
  9. Meile L, Stettler R, Banholzer R, Kotik M, Leisinger T. Tryptophan gene cluster of Methanobacterium thermoautotrophicum Marburg: molecular cloning and nucleotide sequence of a putative trpEGCFBAD operon. J Bacteriol. 1991;173:5017-23 pubmed
    ..With the exception of TrpB, the beta subunit of tryptophan synthase, tryptophan was absent from all Trp polypeptides. ..

More Information


  1. Ma K, Linder D, Stetter K, Thauer R. Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase (coenzyme F420-dependent) from the extreme thermophile Methanopyrus kandleri. Arch Microbiol. 1991;155:593-600 pubmed
    ..The N-terminal amino acid sequence of the reductase from M. kandleri was determined and compared with that of the enzyme from Methanobacterium thermoautotrophicum and Methanosarcina barkeri. Significant similarity was found. ..
  2. Bokranz M, Klein A, Meile L. Complete nucleotide sequence of plasmid pME2001 of Methanobacterium thermoautotrophicum (Marburg). Nucleic Acids Res. 1990;18:363 pubmed
  3. Berk H, Thauer R. F420H2:NADP oxidoreductase from Methanobacterium thermoautotrophicum: identification of the encoding gene via functional overexpression in Escherichia coli. FEBS Lett. 1998;438:124-6 pubmed
    ..The putative gene encoding the enzyme was cloned from Methanobacterium thermoautotrophicum (strain Marburg) and heterologously expressed in Escherichia coli. The overproduced active enzyme was purified, characterized and crystallized. ..
  4. Wasserfallen A, Huber K, Leisinger T. Purification and structural characterization of a flavoprotein induced by iron limitation in Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1995;177:2436-41 pubmed
    ..A possible physiological function for the flavoprotein is discussed. ..
  5. Jenal U, Rechsteiner T, Tan P, Bühlmann E, Meile L, Leisinger T. Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid. J Biol Chem. 1991;266:10570-7 pubmed
    ..Both the mutant and the wild-type ileS gene were expressed in E. coli, and their products displayed the expected difference in sensitivity toward pseudomonic acid. ..
  6. Wongnate T, Ragsdale S. The reaction mechanism of methyl-coenzyme M reductase: how an enzyme enforces strict binding order. J Biol Chem. 2015;290:9322-34 pubmed publisher
    ..This first rapid kinetics study of MCR with its natural substrates describes how an enzyme can enforce a strictly ordered ternary complex mechanism and serves as a template for identification of the reaction intermediates. ..
  7. Thompson H, Tersteegen A, Thauer R, Hedderich R. Two malate dehydrogenases in Methanobacterium thermoautotrophicum. Arch Microbiol. 1998;170:38-42 pubmed
    ..A function of the two malate dehydrogenases in NADPH:NAD+ transhydrogenation is discussed. ..
  8. Hungerer C, Weiss D, Thauer R, Jahn D. The hemA gene encoding glutamyl-tRNA reductase from the archaeon Methanobacterium thermoautotrophicum strain Marburg. Bioorg Med Chem. 1996;4:1089-95 pubmed
    ..0. The reductase utilized preferentially NADPH for the reduction of the activated carboxyl group. The presence of ATP and GTP showed no obvious influence on catalysis. ..
  9. Ding X, Kitagawa Y. Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy. J Biosci Bioeng. 2001;92:488-91 pubmed
    ..In this study, we isolated a water channel-like gene and its flanking sequences from an archaeal genome using only a set of PCR. This method should greatly simplify gene cloning procedures and improve the efficiency of cloning. ..
  10. Bokranz M, Bäumner G, Allmansberger R, Ankel Fuchs D, Klein A. Cloning and characterization of the methyl coenzyme M reductase genes from Methanobacterium thermoautotrophicum. J Bacteriol. 1988;170:568-77 pubmed
    ..Sequence analysis revealed two more open reading frames of unknown function located between two of the methyl coenzyme M reductase genes. ..
  11. Ullmann E, Tan T, Gundinger T, Herwig C, Divne C, Spadiut O. A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis. Biosci Rep. 2014;34:e00167 pubmed publisher
    ..Based on preliminary bioreactor experiments, MmNQO could provide a useful tool to prevent overflow metabolism in applications that require cells with high energy demand. ..
  12. Hedderich R, Berkessel A, Thauer R. Purification and properties of heterodisulfide reductase from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1990;193:255-61 pubmed
    ..2 mM) plus H-S-HTP (app. Km less than 0.05 mM) at a specific rate of 15 The enzyme was highly specific for CoM-S-S-HTP and H-S-CoM plus H-S-HTP. The physiological electron donor/acceptor remains to be identified. ..
  13. Jenal U, Thurner C, Leisinger T. Transcription of the ileS operon in the archaeon Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1993;175:5945-52 pubmed
    ..Extensive decay of the orf401-ileS-purL message was observed. Degradation occurred, presumably by endonucleolytic cleavage, within the orf401 region. ..
  14. Vaupel M, Thauer R. Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase (Mer) from Methanobacterium thermoautotrophicum strain Marburg. Cloning, sequencing, transcriptional analysis, and functional expression in Escherichia coli of the mer gene. Eur J Biochem. 1995;231:773-8 pubmed
    ..Sequence comparisons revealed similarities between the F420-dependent N5,N10-methylenetetrahydromethanopterin reductase and a F420-dependent reductase involved in lincomycin biosynthesis in Streptomyces lincolnensis. ..
  15. Chen A, Poulter C. Isolation and characterization of idsA: the gene for the short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrophicum. Arch Biochem Biophys. 1994;314:399-404 pubmed
    ..The encoded 325-amino-acid protein contained five conserved regions found in eubacterial and eukaryotic farnesyl diphosphate and geranylgeranyl diphosphate synthases, including aspartate-rich motifs commonly found in prenyltransferases. ..
  16. Khandekar S, Eirich L. Purification and characterization of an anabolic fumarate reductase from Methanobacterium thermoautotrophicum. Appl Environ Microbiol. 1989;55:856-61 pubmed
    ..The spectral data, however, suggested the presence of an unknown cofactor tightly bound to the enzyme. Fumarate reductase is involved in the anabolic rather than the catabolic metabolism of M. thermoautotrophicum. ..
  17. Kozono D, Ding X, Iwasaki I, Meng X, Kamagata Y, Agre P, et al. Functional expression and characterization of an archaeal aquaporin. AqpM from methanothermobacter marburgensis. J Biol Chem. 2003;278:10649-56 pubmed publisher
    ..Our studies of archaeal AqpM demonstrate the ubiquity of aquaporins in nature and provide new insight into protein structure and transport selectivity...
  18. Soderberg T, Chen A, Poulter C. Geranylgeranylglyceryl phosphate synthase. Characterization of the recombinant enzyme from Methanobacterium thermoautotrophicum. Biochemistry. 2001;40:14847-54 pubmed
  19. Shima S, Warkentin E, Grabarse W, Sordel M, Wicke M, Thauer R, et al. Structure of coenzyme F(420) dependent methylenetetrahydromethanopterin reductase from two methanogenic archaea. J Mol Biol. 2000;300:935-50 pubmed publisher
    ..However, Mer and MetF do not show sequence similarities although they bind related substrates and catalyze an analogous reaction...
  20. Selmer T, Kahnt J, Goubeaud M, Shima S, Grabarse W, Ermler U, et al. The biosynthesis of methylated amino acids in the active site region of methyl-coenzyme M reductase. J Biol Chem. 2000;275:3755-60 pubmed
    ..A mechanism for the methylation of glutamine at C-2 and of arginine at C-5 is discussed...
  21. Vaupel M, Dietz H, Linder D, Thauer R. Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli. Eur J Biochem. 1996;236:294-300 pubmed
    ..The mch gene was overexpressed in Escherichia coli yielding an active enzyme of 37 kDa with a specific activity of 30 U/mg cell extract protein...
  22. Hippler B, Thauer R. The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH. FEBS Lett. 1999;449:165-8 pubmed
    ..Sequence comparison revealed similarity of MtrH with MetH from Escherichia coli and AcsE from Clostridium thermoaceticum: both enzymes exhibit methyltetrahydrofolate:cob(I)alamin methyltransferase activity...
  23. Mills D, Vitt S, Strauss M, Shima S, Vonck J. De novo modeling of the F(420)-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy. elife. 2013;2:e00218 pubmed publisher
  24. Hochheimer A, Hedderich R, Thauer R. The DNA binding protein Tfx from Methanobacterium thermoautotrophicum: structure, DNA binding properties and transcriptional regulation. Mol Microbiol. 1999;31:641-50 pubmed
    ..thermoautotrophicum in the presence of tung-state. Based on its structure and properties, the DNA binding protein Tfx is proposed to be a transcriptional regulator composed of a basic DNA binding domain and an acidic activation domain...
  25. Wasserfallen A, Ragettli S, Jouanneau Y, Leisinger T. A family of flavoproteins in the domains Archaea and Bacteria. Eur J Biochem. 1998;254:325-32 pubmed
    ..gigas hemoflavoprotein [Gomes, C. M., Silva, G., Oliveira, S., LeGall, J., Liu, M.-Y., Xavier, A. V., Rodrigues-Pousada, C. & Teixeira, M. (1997) J. Biol. Chem. 272, 22502-22508], is discussed...
  26. Stojanowic A, Mander G, Duin E, Hedderich R. Physiological role of the F420-non-reducing hydrogenase (Mvh) from Methanothermobacter marburgensis. Arch Microbiol. 2003;180:194-203 pubmed publisher
  27. Ermler U, Grabarse W, Shima S, Goubeaud M, Thauer R. Crystal structure of methyl-coenzyme M reductase: the key enzyme of biological methane formation. Science. 1997;278:1457-62 pubmed
    ..Together with a second structurally characterized enzyme state (MCRsilent) containing the heterodisulfide of coenzymes M and B, a reaction mechanism is proposed that uses a radical intermediate and a nickel organic compound...
  28. McFarlan S, Terrell C, Hogenkamp H. The purification, characterization, and primary structure of a small redox protein from Methanobacterium thermoautotrophicum, an archaebacterium. J Biol Chem. 1992;267:10561-9 pubmed
    ..This glutaredoxin-like protein may be a component of a ribonucleotide-reducing system distinct from the previously described systems utilizing thioredoxin or glutaredoxin...
  29. Ma K, Thauer R. Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1990;191:187-93 pubmed
    ..3 mM and 3 microM, respectively. Vmax was 6000 protein-1 (kcat = 3600 s-1). The CH2 = H4MPT reductase was stable in the presence of air; at 4 C less than 10% activity was lost within 24 h...
  30. Kiener A, Husain I, Sancar A, Walsh C. Purification and properties of Methanobacterium thermoautotrophicum DNA photolyase. J Biol Chem. 1989;264:13880-7 pubmed
    ..The quantum yield (phi) over this region is constant and is approximately 0.2. The value is measurably smaller than the quantum yields reported for other DNA photolyases...
  31. N lling J, Ishii M, Koch J, Pihl T, Reeve J, Thauer R, et al. Characterization of a 45-kDa flavoprotein and evidence for a rubredoxin, two proteins that could participate in electron transport from H2 to CO2 in methanogenesis in Methanobacterium thermoautotrophicum. Eur J Biochem. 1995;231:628-38 pubmed
    ..abstract truncated at 400 words)..
  32. Hedderich R, Koch J, Linder D, Thauer R. The heterodisulfide reductase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic of pyridine-nucleotide-dependent thioredoxin reductases. Eur J Biochem. 1994;225:253-61 pubmed
    ..46 kDa) did not show sequence similarity to other known proteins, but appears to possess a C-terminal hydrophobic alpha-helix that might function as a membrane anchor. Although hdrB and hdrC are juxtaposed, these genes are not near hdrA...
  33. Setzke E, Hedderich R, Heiden S, Thauer R. H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties. Eur J Biochem. 1994;220:139-48 pubmed
    ..The stimulatory effects of potassium phosphate, a membrane component, uracil derivatives and coenzyme F430 on the H2:heterodisulfide-oxidoreductase activity of the purified complex are described...
  34. Stupperich E, Juza A, Hoppert M, Mayer F. Cloning, sequencing and immunological characterization of the corrinoid-containing subunit of the N5-methyltetrahydromethanopterin: coenzyme-M methyltransferase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1993;217:115-21 pubmed
    ..The N-terminal sequences of mtrC and mtrD are identical with two peptides of the N5-methyltetrahydromethanopterin:coenzyme-M methyltransferase complex from Methanobacterium, indicating that the mtr genes encode this membrane protein...
  35. G rtner P, Ecker A, Fischer R, Linder D, Fuchs G, Thauer R. Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum. Eur J Biochem. 1993;213:537-45 pubmed
    ..The apparent Km for N5-methyltetrahydromethanopterin was 260 microM and that for coenzyme M was 60 microM. The preparation was absolutely dependent on the presence of Ti(III) for activity. ATP enhanced the activity 1.5-2-fold...
  36. Hedderich R, Albracht S, Linder D, Koch J, Thauer R. Isolation and characterization of polyferredoxin from Methanobacterium thermoautotrophicum. The mvhB gene product of the methylviologen-reducing hydrogenase operon. FEBS Lett. 1992;298:65-8 pubmed In the dithionite reduced state the protein displayed an EPR spectrum like that of [4Fe-4S] clusters. The results indicate that the mvhB gene product is indeed a polyferredoxin...
  37. Hochheimer A, Linder D, Thauer R, Hedderich R. The molybdenum formylmethanofuran dehydrogenase operon and the tungsten formylmethanofuran dehydrogenase operon from Methanobacterium thermoautotrophicum. Structures and transcriptional regulation. Eur J Biochem. 1996;242:156-62 pubmed
    ..thermo-autotrophicum revealed that the fwdHFGDACB gene cluster is transcribed in the presence of either molybdate or tungstate in the growth medium whereas the fmdECB gene cluster was only transcribed when molybdate was present...
  38. Tersteegen A, Linder D, Thauer R, Hedderich R. Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum. Eur J Biochem. 1997;244:862-8 pubmed
  39. Chen A, Zhang D, Poulter C. (S)-geranylgeranylglyceryl phosphate synthase. Purification and characterization of the first pathway-specific enzyme in archaebacterial membrane lipid biosynthesis. J Biol Chem. 1993;268:21701-5 pubmed
    ..0 and 7.5. Maximal activity is seen at 50-65 degrees C. The Michaelis constants for GGGP synthase are Vmax = 4.1 +/- 0.5 mumol min-1 mg-1, KMGGPP = 4.1 +/- 1.1 microM, and KMGP = 41 +/- 5 microM...
  40. Grabarse W, Mahlert F, Duin E, Goubeaud M, Shima S, Thauer R, et al. On the mechanism of biological methane formation: structural evidence for conformational changes in methyl-coenzyme M reductase upon substrate binding. J Mol Biol. 2001;309:315-30 pubmed publisher
    ..The three different enzymatically inactive enzyme states are discussed with respect to their enzymatically active precursors and with respect to the catalytic mechanism...
  41. Tersteegen A, Hedderich R. Methanobacterium thermoautotrophicum encodes two multisubunit membrane-bound [NiFe] hydrogenases. Transcription of the operons and sequence analysis of the deduced proteins. Eur J Biochem. 1999;264:930-43 pubmed
    ..A function of these putative membrane-bound [NiFe] hydrogenases as proton pumps involved in endergonic reactions, such as the synthesis of formylmethanofuran from CO2, H2 and methanofuran, is discussed...
  42. Hochheimer A, Schmitz R, Thauer R, Hedderich R. The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide. Eur J Biochem. 1995;234:910-20 pubmed
    ..The fwd operon was found to be located in a region of the M. thermoautotrophicum genome encoding molybdenum enzymes and proteins involved in molybdopterin biosynthesis...
  43. Spreter T, Pech M, Beatrix B. The crystal structure of archaeal nascent polypeptide-associated complex (NAC) reveals a unique fold and the presence of a ubiquitin-associated domain. J Biol Chem. 2005;280:15849-54 pubmed publisher
    ..Based on the presented structure we propose a model for the eukaryotic heterodimeric NAC domain...
  44. Harms U, Weiss D, G rtner P, Linder D, Thauer R. The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits. Eur J Biochem. 1995;228:640-8 pubmed
    ..The mtr operon was found to be located between the methyl-coenzyme M reductase I operon (mcr) and a downstream open reading frame predicted to encode a Na+/Ca2+, K+ exchanger...
  45. Eberhardt S, Korn S, Lottspeich F, Bacher A. Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum. J Bacteriol. 1997;179:2938-43 pubmed
    ..The 5' phosphate of 6,7-dimethyl-8-ribityllumazine does not act as a substrate. The findings suggest that riboflavin synthase has evolved independently in eubacteria and methanobacteria...
  46. Rospert S, Linder D, Ellermann J, Thauer R. Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H. Eur J Biochem. 1990;194:871-7 pubmed
    ..When the cultures were supplied with sufficient H2 and and CO2 and the cells grew exponentially, essentially only MCR II was found. When growth was limited by the gas supply, MCR I predominated...
  47. Heim S, K nkel A, Thauer R, Hedderich R. Thiol:fumarate reductase (Tfr) from Methanobacterium thermoautotrophicum--identification of the catalytic sites for fumarate reduction and thiol oxidation. Eur J Biochem. 1998;253:292-9 pubmed