sea vase


Alias: yellow sea squirt, vase tunicate, Ciona intestinalis, Ascidia intestinalis, Ciona intestinalis (Linnaeus, 1767)

Top Publications

  1. Takamura K, Egawa T, Ohnishi S, Okada T, Fukuoka T. Developmental expression of ascidian neurotransmitter synthesis genes. I. Choline acetyltransferase and acetylcholine transporter genes. Dev Genes Evol. 2002;212:50-3 pubmed
    To identify cholinergic neurons, we isolated a choline acetyltransferase (Ci-ChAT) gene from Ciona intestinalis by PCR methods. In the cloning process, we also obtained the gene encoding the vesicular acetylcholine transporter (Ci-vAChTP)...
  2. Yoshida R, Sakurai D, Horie T, Kawakami I, Tsuda M, Kusakabe T. Identification of neuron-specific promoters in Ciona intestinalis. Genesis. 2004;39:130-40 pubmed
    ..each of which is expressed in distinct sets of neurons in the central nervous system of the ascidian Ciona intestinalis, and we examined their function by introducing green fluorescent protein (GFP)-fusion constructs into Ciona ..
  3. Murata Y, Iwasaki H, Sasaki M, Inaba K, Okamura Y. Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. Nature. 2005;435:1239-43 pubmed
    ..Here we describe a novel protein from the ascidian Ciona intestinalis that has a transmembrane voltage-sensing domain homologous to the S1-S4 segments of voltage-gated channels ..
  4. Passamaneck Y, Katikala L, Perrone L, Dunn M, Oda Ishii I, Di Gregorio A. Direct activation of a notochord cis-regulatory module by Brachyury and FoxA in the ascidian Ciona intestinalis. Development. 2009;136:3679-89 pubmed publisher easily testable model for such investigations in a 155-bp notochord-specific CRM from the ascidian Ciona intestinalis. This CRM contains functional binding sites for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a)...
  5. Vandenberghe A, Meedel T, Hastings K. mRNA 5'-leader trans-splicing in the chordates. Genes Dev. 2001;15:294-303 pubmed
    ..In the ascidian protochordate Ciona intestinalis, the mRNAs of at least seven genes undergo trans-splicing of a 16-nucleotide 5'-leader apparently derived ..
  6. Oda Ishii I, Bertrand V, Matsuo I, Lemaire P, Saiga H. Making very similar embryos with divergent genomes: conservation of regulatory mechanisms of Otx between the ascidians Halocynthia roretzi and Ciona intestinalis. Development. 2005;132:1663-74 pubmed almost perfectly conserved, even between the evolutionarily distant species Halocynthia roretzi and Ciona intestinalis, which show no detectable sequence conservation in the non-coding regions of studied orthologous genes...
  7. Zega G, Biggiogero M, Groppelli S, Candiani S, Oliveri D, Parodi M, et al. Developmental expression of glutamic acid decarboxylase and of gamma-aminobutyric acid type B receptors in the ascidian Ciona intestinalis. J Comp Neurol. 2008;506:489-505 pubmed
    We describe Ciona intestinalis gamma-aminobutyric acid (GABA)-ergic neurons during development, studying the expression pattern of Ci-GAD (glutamic acid decarboxylase: GABA synthesizing enzyme) by in situ hybridization...
  8. Chiba S, Jiang D, Satoh N, Smith W. Brachyury null mutant-induced defects in juvenile ascidian endodermal organs. Development. 2009;136:35-9 pubmed publisher
    We report the isolation of a recessive ENU-induced short-tailed mutant in the ascidian Ciona intestinalis that is the product of a premature stop in the brachyury gene...
  9. Corbo J, Levine M, Zeller R. Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis. Development. 1997;124:589-602 pubmed
    We present evidence that the embryo of the ascidian, Ciona intestinalis, is an easily manipulated system for investigating the establishment of basic chordate tissues and organs...

More Information

Publications178 found, 100 shown here

  1. Dehal P, Satou Y, Campbell R, Chapman J, Degnan B, De Tomaso A, et al. The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins. Science. 2002;298:2157-67 pubmed
    ..vertebrates, we generated a draft of the protein-coding portion of the genome of the most studied ascidian, Ciona intestinalis. The Ciona genome contains approximately 16,000 protein-coding genes, similar to the number in other ..
  2. Yagi K, Satou Y, Mazet F, Shimeld S, Degnan B, Rokhsar D, et al. A genomewide survey of developmentally relevant genes in Ciona intestinalis. III. Genes for Fox, ETS, nuclear receptors and NFkappaB. Dev Genes Evol. 2003;213:235-44 pubmed
    A survey against the draft genome sequence and the cDNA/EST database of Ciona intestinalis identified a number of genes encoding transcription factors regulating a variety of processes including development...
  3. Satou Y, Hamaguchi M, Takeuchi K, Hastings K, Satoh N. Genomic overview of mRNA 5'-leader trans-splicing in the ascidian Ciona intestinalis. Nucleic Acids Res. 2006;34:3378-88 pubmed
    Although spliced leader (SL) trans-splicing in the chordates was discovered in the tunicate Ciona intestinalis there has been no genomic overview analysis of the extent of trans-splicing or the make-up of the trans-spliced and non-trans-..
  4. Rothbächer U, Bertrand V, Lamy C, Lemaire P. A combinatorial code of maternal GATA, Ets and beta-catenin-TCF transcription factors specifies and patterns the early ascidian ectoderm. Development. 2007;134:4023-32 pubmed
    ..We thus identify a precise combinatorial code of maternal factors responsible for zygotic onset of a chordate ectodermal genetic program. ..
  5. Villalba Galea C, Sandtner W, Starace D, Bezanilla F. S4-based voltage sensors have three major conformations. Proc Natl Acad Sci U S A. 2008;105:17600-7 pubmed publisher
  6. Stolfi A, Gainous T, Young J, Mori A, Levine M, Christiaen L. Early chordate origins of the vertebrate second heart field. Science. 2010;329:565-8 pubmed publisher
    ..We found that heart progenitor cells of the simple chordate Ciona intestinalis also generate precursors of the atrial siphon muscles (ASMs)...
  7. Ogasawara M, Sasaki M, Nakazawa N, Nishino A, Okamura Y. Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian. Gene Expr Patterns. 2011;11:233-8 pubmed publisher
    ..pattern of ascidian VSP(Ci-VSP) was examined in embryos and juveniles of a marine invertebrate chordate, Ciona intestinalis. RT-PCR showed that Ci-VSP is expressed at the larval stage and that expression persists in juveniles...
  8. Ragkousi K, Beh J, Sweeney S, Starobinska E, Davidson B. A single GATA factor plays discrete, lineage specific roles in ascidian heart development. Dev Biol. 2011;352:154-63 pubmed publisher
    ..Early heart specification in the invertebrate chordate Ciona intestinalis is similar to that of vertebrates but only one GATA factor, Ci-GATAa, is expressed in the heart progenitor ..
  9. Joyce Tang W, Chen J, Zeller R. Transcriptional regulation of the peripheral nervous system in Ciona intestinalis. Dev Biol. 2013;378:183-93 pubmed publisher
    ..we investigated the roles played by PNG homologs in regulating PNS development of the invertebrate chordate Ciona intestinalis. We discovered that in Ciona, MyT1, Pou4, Atonal, and NeuroD-like are expressed in a sequential regulatory ..
  10. Wang W, Razy Krajka F, Siu E, Ketcham A, Christiaen L. NK4 antagonizes Tbx1/10 to promote cardiac versus pharyngeal muscle fate in the ascidian second heart field. PLoS Biol. 2013;11:e1001725 pubmed publisher
    ..We propose that this basic ontogenetic motif underlies cardiac and pharyngeal muscle development and evolution in chordates. ..
  11. Di Gregorio A, Levine M. Regulation of Ci-tropomyosin-like, a Brachyury target gene in the ascidian, Ciona intestinalis. Development. 1999;126:5599-609 pubmed
    ..In ascidians, such as Ciona intestinalis, Brachyury is expressed exclusively in the notochord and does not exhibit an early pan-mesodermal pattern...
  12. Wada H, Kobayashi M, Sato R, Satoh N, Miyasaka H, Shirayama Y. Dynamic insertion-deletion of introns in deuterostome EF-1alpha genes. J Mol Evol. 2002;54:118-28 pubmed
    ..These analyses revealed that the deuterostome EF-1alpha gene has lost individual introns more frequently than all introns simultaneously. ..
  13. Nakashima Y, Kusakabe T, Kusakabe R, Terakita A, Shichida Y, Tsuda M. Origin of the vertebrate visual cycle: genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate. J Comp Neurol. 2003;460:180-90 pubmed
    ..protein (CRALBP) and beta-carotene 15,15'-monooxygenase (BCO)/RPE65 in a primitive chordate, ascidian Ciona intestinalis. The mRNAs for these proteins are specifically expressed in the central nervous system during embryonic ..
  14. Adams B, Tello J, Erchegyi J, Warby C, Hong D, Akinsanya K, et al. Six novel gonadotropin-releasing hormones are encoded as triplets on each of two genes in the protochordate, Ciona intestinalis. Endocrinology. 2003;144:1907-19 pubmed
    ..We have identified two genes encoding GnRH in a protochordate, Ciona intestinalis, thought to be related to the ancestral animal that gave rise to vertebrates...
  15. Tello J, Rivier J, Sherwood N. Tunicate gonadotropin-releasing hormone (GnRH) peptides selectively activate Ciona intestinalis GnRH receptors and the green monkey type II GnRH receptor. Endocrinology. 2005;146:4061-73 pubmed
    ..have GnRH peptides because we previously reported six GnRH peptides encoded within the tunicate genome of Ciona intestinalis. Here we clone and sequence cDNAs for four putative GnRH receptors from C. intestinalis...
  16. Moret F, Christiaen L, Deyts C, Blin M, Joly J, Vernier P. The dopamine-synthesizing cells in the swimming larva of the tunicate Ciona intestinalis are located only in the hypothalamus-related domain of the sensory vesicle. Eur J Neurosci. 2005;21:3043-55 pubmed
    ..a high-resolution cellular analysis of tyrosine hydroxylase (TH)-positive and dopamine-positive cells in Ciona intestinalis embryos and larvae...
  17. Dufour H, Chettouh Z, Deyts C, de Rosa R, Goridis C, Joly J, et al. Precraniate origin of cranial motoneurons. Proc Natl Acad Sci U S A. 2006;103:8727-32 pubmed
    ..The motoneurons of adult Ciona intestinalis, located in the cerebral ganglion and innervating muscles associated with the huge "branchial basket,&..
  18. Hudson C, Yasuo H. A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in Ciona embryos. Development. 2006;133:2855-64 pubmed
    ..We show that specification of the secondary but not the primary notochord in Ciona intestinalis requires a relay mechanism involving two signalling pathways...
  19. Mita K, Fujiwara S. Nodal regulates neural tube formation in the Ciona intestinalis embryo. Dev Genes Evol. 2007;217:593-601 pubmed
    ..of a lefty orthologue, Ci-lefty, caused a failure of neural tube closure in the protochordate ascidian Ciona intestinalis. The body bent dorsally, and anterior-posterior elongation was inhibited...
  20. Shchedrina V, Novoselov S, Malinouski M, Gladyshev V. Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif. Proc Natl Acad Sci U S A. 2007;104:13919-24 pubmed publisher
    ..In a bacterial expression system, we directly demonstrated the formation of a diselenide bond between the two Sec, establishing it as the first diselenide bond found in a natural protein...
  21. Horie T, Sakurai D, Ohtsuki H, Terakita A, Shichida Y, Usukura J, et al. Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva. J Comp Neurol. 2008;509:88-102 pubmed publisher
    ..opsin, Ci-opsin1, is localized in the outer segments of the photoreceptor cells of larvae of the ascidian Ciona intestinalis. The absorption spectrum of the photopigment reconstituted from Ci-opsin1 and 11-cis-retinal suggested that ..
  22. Iwasaki H, Murata Y, Kim Y, Hossain M, Worby C, Dixon J, et al. A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate. Proc Natl Acad Sci U S A. 2008;105:7970-5 pubmed publisher
    ..The enzymatic activity of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), recently identified as a member of the PTEN (phosphatase and ..
  23. Dunn M, Di Gregorio A. The evolutionarily conserved leprecan gene: its regulation by Brachyury and its role in the developing Ciona notochord. Dev Biol. 2009;328:561-74 pubmed publisher
    In Ciona intestinalis, leprecan was identified as a target of the notochord-specific transcription factor Ciona Brachyury (Ci-Bra) (Takahashi, H., Hotta, K., Erives, A., Di Gregorio, A., Zeller, R.W., Levine, M., Satoh, N., 1999...
  24. Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, et al. Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis. Development. 2012;139:2156-60 pubmed publisher
    ..Here, we show that in the urochordate ascidian Ciona intestinalis, RA-Hox1 in the epidermal ectoderm is necessary for formation of the atrial siphon placode (ASP), a ..
  25. José Edwards D, Oda Ishii I, Nibu Y, Di Gregorio A. Tbx2/3 is an essential mediator within the Brachyury gene network during Ciona notochord development. Development. 2013;140:2422-33 pubmed publisher
    ..This work sheds light on a large section of the notochord regulatory circuitry controlled by T-box factors, and reveals new components of the complement of genes required for the proper formation of this structure...
  26. Gionti M, Ristoratore F, Di Gregorio A, Aniello F, Branno M, Di Lauro R. Cihox5, a new Ciona intestinalis Hox-related gene, is involved in regionalization of the spinal cord. Dev Genes Evol. 1998;207:515-23 pubmed
    In this paper we report the cloning, sequence and expression analysis of a new Ciona intestinalis hox gene. On the basis of sequence comparison with mammalian and Amphioxus homologues, we called this gene Cihox5...
  27. Hudson C, Lemaire P. Induction of anterior neural fates in the ascidian Ciona intestinalis. Mech Dev. 2001;100:189-203 pubmed
    ..Here we report the isolation of two sensory vesicle markers in the ascidian Ciona intestinalis, which are homologs of vertebrate otx and gsx homeobox genes...
  28. Hudson C, Darras S, Caillol D, Yasuo H, Lemaire P. A conserved role for the MEK signalling pathway in neural tissue specification and posteriorisation in the invertebrate chordate, the ascidian Ciona intestinalis. Development. 2003;130:147-59 pubmed
    ..analysed the role of the Ras/MEK/ERK signalling pathway in the formation of the larval CNS in the ascidian, Ciona intestinalis. We show evidence that this pathway is required, during the cleavage stages, for the acquisition of: (1) ..
  29. Okamura Y, Nishino A, Murata Y, Nakajo K, Iwasaki H, Ohtsuka Y, et al. Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes. Physiol Genomics. 2005;22:269-82 pubmed
    ..Here we systematically survey up to 160 putative ion channel genes in the genome of Ciona intestinalis and compare them with corresponding gene sets from the genomes of the nematode Chaenorhabditis elegans, the ..
  30. Beh J, Shi W, Levine M, Davidson B, Christiaen L. FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. Development. 2007;134:3297-305 pubmed
    ..Taken together, our results indicate that FoxF is a direct target of FGF signaling and is predominantly involved in the regulation of heart cell migration. ..
  31. Horie T, Kusakabe T, Tsuda M. Glutamatergic networks in the Ciona intestinalis larva. J Comp Neurol. 2008;508:249-63 pubmed publisher
    ..the vesicular glutamate transporter subfamily, a specific marker of glutamatergic neurons, in the ascidian Ciona intestinalis. The Ci-VGLUT gene is expressed in the adhesive organ, the epidermal neurons, and the brain vesicle, but not ..
  32. Kusakabe T, Kusakabe R, Kawakami I, Satou Y, Satoh N, Tsuda M. Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis. FEBS Lett. 2001;506:69-72 pubmed
    A novel gene encoding visual pigment, Ci-opsin1, was identified in a primitive chordate, the ascidian, Ciona intestinalis. Molecular phylogenetic analysis and the exon-intron organization suggest that Ci-opsin1 is closely related to the ..
  33. Wang J, Karabinos A, Zimek A, Meyer M, Riemer D, Hudson C, et al. Cytoplasmic intermediate filament protein expression in tunicate development: a specific marker for the test cells. Eur J Cell Biol. 2002;81:302-11 pubmed
    The urochordate Ciona intestinalis is a well established system for embryological studies, and large scale EST sequences begin to emerge...
  34. Khalturin K, Panzer Z, Cooper M, Bosch T. Recognition strategies in the innate immune system of ancestral chordates. Mol Immunol. 2004;41:1077-87 pubmed publisher
    ..To understand better the evolution of adaptive immune systems, more comparative data from jawless vertebrates (lamprey or hagfish) and a representative of Acrania (e.g. Amphioxus) are clearly needed...
  35. Mishina Y, Mutoh H, Song C, Knöpfel T. Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain. Front Mol Neurosci. 2014;7:78 pubmed publisher
    ..termed VSFP-Butterflies) and also demonstrated a series of chimeric VSD in which portions of the VSD of Ciona intestinalis voltage-sensitive phosphatase are substituted by homologous portions of a voltage-gated potassium channel ..
  36. Spagnuolo A, Ristoratore F, Di Gregorio A, Aniello F, Branno M, Di Lauro R. Unusual number and genomic organization of Hox genes in the tunicate Ciona intestinalis. Gene. 2003;309:71-9 pubmed
    ..We report in this paper that the ascidian Ciona intestinalis genome appears to contain a single Hox gene complex which shows absence of some of the members found in all ..
  37. Jiang L, Liu Q, Ni J. In silico identification of the sea squirt selenoproteome. BMC Genomics. 2010;11:289 pubmed publisher
    ..Using this method, eighteen selenoprotein genes were identified from the genome of Ciona intestinalis, leading to its member of selenoproteome up to 19...
  38. Berthier C, Kutchukian C, Bouvard C, Okamura Y, Jacquemond V. Depression of voltage-activated Ca2+ release in skeletal muscle by activation of a voltage-sensing phosphatase. J Gen Physiol. 2015;145:315-30 pubmed publisher
    ..E-C) coupling of mouse muscle fibers using in vivo expression of the voltage-sensing phosphatases (VSPs) Ciona intestinalis VSP (Ci-VSP) or Danio rerio VSP (Dr-VSP)...
  39. Sung U, Sepehri Rad M, Piao H, Jin L, Hughes T, Cohen L, et al. Developing Fast Fluorescent Protein Voltage Sensors by Optimizing FRET Interactions. PLoS ONE. 2015;10:e0141585 pubmed publisher
    ..sensors by optimizing the location of donor and acceptor FPs flanking the voltage sensitive domain of the Ciona intestinalis voltage sensitive phosphatase...
  40. Hayashibe M, Nakayama S, Ogasawara M. Shared hemocyte- and intestine-dominant expression profiles of intelectin genes in ascidian Ciona intestinalis: insight into the evolution of the innate immune system in chordates. Cell Tissue Res. 2017;370:129-142 pubmed publisher
    ..domain and an intelectin domain and an additional sequence has been reported only in ascidian Ciona intestinalis intelectins...
  41. Harada Y, Takagaki Y, Sunagawa M, Saito T, Yamada L, Taniguchi H, et al. Mechanism of self-sterility in a hermaphroditic chordate. Science. 2008;320:548-50 pubmed publisher
    ..A primitive chordate (ascidian) Ciona intestinalis is an example of such an organism, but the molecular mechanism underlying its SI system is not known...
  42. Stolfi A, Gandhi S, Salek F, Christiaen L. Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014;141:4115-20 pubmed publisher
    ..Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of the Ebf (Collier/..
  43. Le Goff E, Martinand Mari C, Martin M, Feuillard J, Boublik Y, Godefroy N, et al. Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis. Biol Open. 2015;4:1109-21 pubmed publisher
    ..Here, we studied the ascidian Ciona intestinalis counterpart of E(z)...
  44. Cota C, Davidson B. Mitotic Membrane Turnover Coordinates Differential Induction of the Heart Progenitor Lineage. Dev Cell. 2015;34:505-19 pubmed publisher
    ..We investigate in vivo signaling dynamics using the invertebrate chordate, Ciona intestinalis. In Ciona, matrix adhesion polarizes fibroblast growth factor (FGF)-dependent heart progenitor induction...
  45. Salvador Martínez I, Salazar Ciudad I. How complexity increases in development: An analysis of the spatial-temporal dynamics of Gene expression in Ciona intestinalis. Mech Dev. 2017;144:113-124 pubmed publisher
    ..Here, we present a quantitative analysis of the compartmentalization and spatial complexity of Ciona intestinalis over developmental time by analyzing thousands of gene expression spatial patterns from the ANISEED database...
  46. Satou Y, Yagi K, Imai K, Yamada L, Nishida H, Satoh N. macho-1-Related genes in Ciona embryos. Dev Genes Evol. 2002;212:87-92 pubmed publisher
    ..Here we have characterized macho-1 homologues, Ci-macho1 of Ciona intestinalis and Cs-macho1 of Ciona savignyi...
  47. Karabinos A, Zimek A, Weber K. The genome of the early chordate Ciona intestinalis encodes only five cytoplasmic intermediate filament proteins including a single type I and type II keratin and a unique IF-annexin fusion protein. Gene. 2004;326:123-9 pubmed
    We screened the recently established draft genome of the early chordate Ciona intestinalis for genes encoding cytoplasmic intermediate filament (IF) proteins...
  48. Kawasaki K, Suzuki T, Weiss K. Genetic basis for the evolution of vertebrate mineralized tissue. Proc Natl Acad Sci U S A. 2004;101:11356-61 pubmed publisher
    ..We also suggest that mammalian enamel is distinct from fish enameloid. Their similar nature as a hard structural overlay on exoskeleton and teeth is because of convergent evolution...
  49. Kobayashi M, Takatori N, Nakajima Y, Kumano G, Nishida H, Saiga H. Spatial and temporal expression of two transcriptional isoforms of Lhx3, a LIM class homeobox gene, during embryogenesis of two phylogenetically remote ascidians, Halocynthia roretzi and Ciona intestinalis. Gene Expr Patterns. 2010;10:98-104 pubmed publisher
    ..that two transcriptional isoforms a and b are differentially expressed during the larval development of Ciona intestinalis (Christiaen et al., 2009)...
  50. Yoshiyama Yanagawa T, Enya S, Shimada Niwa Y, Yaguchi S, Haramoto Y, Matsuya T, et al. The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme. J Biol Chem. 2011;286:25756-62 pubmed publisher
    ..dafachronic acids or ecdysteroids, including the sea urchin Hemicentrotus pulcherrimus, the sea squirt Ciona intestinalis, the fish Danio rerio, and the frog Xenopus laevis...
  51. Vizzini A, Parrinello D, Sanfratello M, Trapani M, Mangano V, Parrinello N, et al. Upregulated transcription of phenoloxidase genes in the pharynx and endostyle of Ciona intestinalis in response to LPS. J Invertebr Pathol. 2015;126:6-11 pubmed publisher
    ..In Ciona intestinalis two phenoloxidases (CinPO-1, CinPO-2) have been sequenced...
  52. Stolfi A, Ryan K, Meinertzhagen I, Christiaen L. Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature. 2015;527:371-4 pubmed publisher
    ..Here we show that a particular neuronal cell type in the tadpole larva of the tunicate Ciona intestinalis, the bipolar tail neuron, shares a set of features with neural-crest-derived spinal ganglia neurons in ..
  53. Corbo J, Fujiwara S, Levine M, Di Gregorio A. Suppressor of hairless activates brachyury expression in the Ciona embryo. Dev Biol. 1998;203:358-68 pubmed publisher
    ..Expression of a constitutively activated Xotch receptor in the notochord, endoderm, and CNS also alters tail morphogenesis. These results suggest that a Notch-Su(H) pathway might participate in notochord differentiation in Ciona...
  54. Takatori N, Satou Y, Satoh N. Expression of hedgehog genes in Ciona intestinalis embryos. Mech Dev. 2002;116:235-8 pubmed
    ..The present study revealed that Ciona intestinalis contains two hedgehog genes (Ci-hh1 and Ci-hh2), which are likely to be independent duplicate genes in this ..
  55. Ogasawara M, Satou Y. Expression of FoxE and FoxQ genes in the endostyle of Ciona intestinalis. Dev Genes Evol. 2003;213:416-9 pubmed
    We have analyzed the expression patterns of two Fox genes, FoxE and FoxQ, in the ascidian Ciona intestinalis. Expression of Ci-FoxE was specific to the endostyle of adults, being prominent in the thyroid-equivalent region of zone 7...
  56. K rn U, Sommer F, Bosch T, Khalturin K. In the urochordate Ciona intestinalis zona pellucida domain proteins vary among individuals. Dev Comp Immunol. 2007;31:1242-54 pubmed publisher
    ..By comparing gonadal transcripts of three genetically unrelated Ciona intestinalis individuals by suppression subtractive hybridization (SSH), we have previously identified a highly ..
  57. Parrinello N, Vizzini A, Arizza V, Salerno G, Parrinello D, Cammarata M, et al. Enhanced expression of a cloned and sequenced Ciona intestinalis TNFalpha-like (CiTNF alpha) gene during the LPS-induced inflammatory response. Cell Tissue Res. 2008;334:305-17 pubmed publisher
    A tumor necrosis factor-alpha (TNFalpha)-like gene from Ciona intestinalis (CiTNF alpha-like) body wall challenged with bacterial lipopolysaccharide (LPS) was cloned and sequenced 4 h after LPS inoculation...
  58. Nishikata T, Wada M. Molecular characterization of myoplasmin-C1: a cytoskeletal component localized in the myoplasm of the ascidian egg. Dev Genes Evol. 1996;206:72-9 pubmed publisher
    ..These results suggest that myoplasmin-C1 is a cytoskeletal component of the myoplasm and that it plays a role in anchoring and segregating muscle determinants. ..
  59. José Edwards D, Oda Ishii I, Kugler J, Passamaneck Y, Katikala L, Nibu Y, et al. Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 2015;11:e1005730 pubmed publisher
    ..Yet, this study uncovered essential elements recurrently used by divergent chordates as basic building blocks for notochord CRMs. ..
  60. Rodrigues A, Camargo A, Andjelković A, Jacobs H, Oliveira M. Developmental arrest in Drosophila melanogaster caused by mitochondrial DNA replication defects cannot be rescued by the alternative oxidase. Sci Rep. 2018;8:10882 pubmed publisher
    The xenotopic expression of the alternative oxidase AOX from the tunicate Ciona intestinalis in diverse models of human disease partially alleviates the phenotypic effects of mitochondrial respiratory chain defects...
  61. Keys D, Lee B, Di Gregorio A, Harafuji N, Detter J, Wang M, et al. A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis. Proc Natl Acad Sci U S A. 2005;102:679-83 pubmed publisher
    ..within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes ..
  62. Kürn U, Sommer F, Hemmrich G, Bosch T, Khalturin K. Allorecognition in urochordates: identification of a highly variable complement receptor-like protein expressed in follicle cells of Ciona. Dev Comp Immunol. 2007;31:360-71 pubmed
    ..involved in preventing self fertilization, we compared gonadal cDNAs of three genetically unrelated Ciona intestinalis individuals by suppression subtractive hybridisation (SSH)...
  63. Brozovic M, Martin C, Dantec C, Dauga D, Mendez M, Simion P, et al. ANISEED 2015: a digital framework for the comparative developmental biology of ascidians. Nucleic Acids Res. 2016;44:D808-18 pubmed publisher
    ..This new architecture was used to improve and enrich the description of Ciona intestinalis embryonic development, based on an improved genome assembly and gene model set, refined functional gene ..
  64. Filip N, Pustam A, Ells V, Grosicki K, Yang J, Oguejiofor I, et al. Fouling-release and chemical activity effects of a siloxane-based material on tunicates. Mar Environ Res. 2016;116:41-50 pubmed publisher
    ..In field immersion trials, the EIM caused the greatest reduction in fouling by the solitary tunicate Ciona intestinalis and caused the longest delay in the progression of fouling by two species of colonial tunicate...
  65. Kari W, Zeng F, Zitzelsberger L, Will J, Rothbächer U. Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis. J Vis Exp. 2016;: pubmed publisher
    ..The chordate Ciona intestinalis is an invertebrate chordate that emerged from a common ancestor with the vertebrates and may represent ..
  66. Tzouanacou E, Tweedie S, Wilson V. Identification of Jade1, a gene encoding a PHD zinc finger protein, in a gene trap mutagenesis screen for genes involved in anteroposterior axis development. Mol Cell Biol. 2003;23:8553-2 pubmed
    ..Database searches identified a family of Jade proteins conserved through vertebrates. This raises the possibility that the absence of phenotype is due to a functional compensation by other family members...
  67. Horn R. Electrifying phosphatases. Sci STKE. 2005;2005:pe50 pubmed publisher
    ..How the voltage-sensing (VS) domain of Ci-VSP communicates with the phosphatase domain, and how the VS domain moves its charges across the membrane electric field in the absence of a transmembrane pore domain, remain to be determined...
  68. Kawada T, Ogasawara M, Sekiguchi T, Aoyama M, Hotta K, Oka K, et al. Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis: homologs and prototypes of vertebrate peptides and novel peptides. Endocrinology. 2011;152:2416-27 pubmed publisher
    ..In the present study, we have identified various peptides in the ascidian, Ciona intestinalis. Mass spectrometry-based peptidomic analysis detected 33 peptides, including 26 novel peptides, from C...
  69. Vizzini A, Bonura A, Longo V, Sanfratello M, Parrinello D, Cammarata M, et al. Isolation of a novel LPS-induced component of the ML superfamily in Ciona intestinalis. Dev Comp Immunol. 2015;53:70-8 pubmed publisher
    ..In this study, we report the identification of the first component of the ML superfamily in the invertebrate Ciona intestinalis by means of a subtractive hybridization strategy...
  70. Garstang M, Osborne P, Ferrier D. TCF/Lef regulates the Gsx ParaHox gene in central nervous system development in chordates. BMC Evol Biol. 2016;16:57 pubmed publisher
    ..Using Ciona intestinalis transgenics, we isolated a regulatory element upstream of Branchiostoma floridae Gsx that drives expression ..
  71. Tokuoka M, Imai K, Satou Y, Satoh N. Three distinct lineages of mesenchymal cells in Ciona intestinalis embryos demonstrated by specific gene expression. Dev Biol. 2004;274:211-24 pubmed
    ..These results provide evidence that the differentiation of each of the three mesenchyme lineages of Ciona embryos is characterized by the expression of a specific set of genes, whose expression is controlled differentially. ..
  72. Khalturin K, K rn U, Pinnow N, Bosch T. Towards a molecular code for individuality in the absence of MHC: screening for individually variable genes in the urochordate Ciona intestinalis. Dev Comp Immunol. 2005;29:759-73 pubmed publisher
    ..Using suppression subtractive hybridisation (SSH) we surveyed Ciona intestinalis for individually variable genes...
  73. DeMarco R, Venancio T, Verjovski Almeida S. SmTRC1, a novel Schistosoma mansoni DNA transposon, discloses new families of animal and fungi transposons belonging to the CACTA superfamily. BMC Evol Biol. 2006;6:89 pubmed publisher
  74. Fedders H, Michalek M, Gr tzinger J, Leippe M. An exceptional salt-tolerant antimicrobial peptide derived from a novel gene family of haemocytes of the marine invertebrate Ciona intestinalis. Biochem J. 2008;416:65-75 pubmed publisher
    ..antimicrobial peptides was identified in the EST (expressed sequence tag) database of the sea squirt Ciona intestinalis, and one of these genes was molecularly cloned from the Northern European Ciona subspecies...
  75. Ryan K, Lu Z, Meinertzhagen I. Circuit Homology between Decussating Pathways in the Ciona Larval CNS and the Vertebrate Startle-Response Pathway. Curr Biol. 2017;27:721-728 pubmed publisher
    ..2], we have compiled a densely reconstructed connectome (defined in [3]) for the CNS in the tadpole larva of Ciona intestinalis (L.), using ssEM [4]...
  76. Nakayama S, Ogasawara M. Compartmentalized expression patterns of pancreatic- and gastric-related genes in the alimentary canal of the ascidian Ciona intestinalis: evolutionary insights into the functional regionality of the gastrointestinal tract in Olfactores. Cell Tissue Res. 2017;370:113-128 pubmed publisher
    ..Spatial expression analyses in the ascidian Ciona intestinalis, by means of whole-mount in situ hybridization, have elucidated that the expression of Ciona homologs of ..
  77. Holzner S, Senfter D, Stadler S, Staribacher A, Nguyen C, Gaggl A, et al. Colorectal cancer cell-derived microRNA200 modulates the resistance of adjacent blood endothelial barriers in vitro. Oncol Rep. 2016;36:3065-3071 pubmed publisher
  78. Corbo J, Erives A, Di Gregorio A, Chang A, Levine M. Dorsoventral patterning of the vertebrate neural tube is conserved in a protochordate. Development. 1997;124:2335-44 pubmed
    ..Here we analyze the expression of HNF-3beta and snail homologues in the ascidian, Ciona intestinalis, a member of the subphylum Urochordata, the earliest branch in the chordate phylum...
  79. Kawashima T, Murakami A, Ogasawara M, Tanaka K, Isoda R, Sasakura Y, et al. Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis. Dev Genes Evol. 2000;210:162-5 pubmed
    ..cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus ..
  80. Immesberger A, Burmester T. Putative phenoloxidases in the tunicate Ciona intestinalis and the origin of the arthropod hemocyanin superfamily. J Comp Physiol B. 2004;174:169-80 pubmed publisher
    ..However, in their analysis of the predicted genes in the Ciona intestinalis (Urochordata:Tunicata) genome, Dehal et al...
  81. Norden Krichmar T, Holtz J, Pasquinelli A, Gaasterland T. Computational prediction and experimental validation of Ciona intestinalis microRNA genes. BMC Genomics. 2007;8:445 pubmed publisher
    This study reports the first collection of validated microRNA genes in the sea squirt, Ciona intestinalis. MicroRNAs are processed from hairpin precursors to ~22 nucleotide RNAs that base pair to target mRNAs and inhibit expression...
  82. Wu Z, Wang K, Wang L, Feng J, Hao S, Tian K, et al. The brachyury Gly177Asp SNP is not associated with a risk of skull base chordoma in the Chinese population. Int J Mol Sci. 2013;14:21258-65 pubmed publisher
    ..Our data suggest that the brachyury Gly177Asp SNP is not involved in the risks of skull-base chordoma, at least in the Chinese population. ..
  83. Gline S, Kaplan N, Bernadskaya Y, Abdu Y, Christiaen L. Surrounding tissues canalize motile cardiopharyngeal progenitors towards collective polarity and directed migration. Development. 2015;142:544-54 pubmed publisher
    ..The cardiogenic progenitors (also known as trunk ventral cells, TVCs) of the ascidian Ciona intestinalis provide a simple chordate model with which to study collective migration...
  84. Nakazawa S, Shirae Kurabayashi M, Otsuka K, Sawada H. Proteomics of ionomycin-induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian Ciona intestinalis. Proteomics. 2015;15:4064-79 pubmed publisher
    ..exudates and sperm surface proteins using ionomycin-induced sperm reaction and cell-impermeable labeling in Ciona intestinalis type A (C. robusta)...
  85. Veeman M. Covert Prepatterning of a Cell Division Wave. Dev Cell. 2016;37:107-8 pubmed publisher anterior across the epidermis, is important for neural tube closure in the invertebrate chordate Ciona intestinalis. In this issue of Developmental Cell, Ogura and Sasakura (2016) show that the patterning of this wave ..
  86. Lopez C, Sheehan H, Vierra D, Azzinaro P, Meedel T, Howlett N, et al. Proteomic responses to elevated ocean temperature in ovaries of the ascidian Ciona intestinalis. Biol Open. 2017;6:943-955 pubmed publisher
    i>Ciona intestinalis, a common sea squirt, exhibits lower reproductive success at the upper extreme of the water temperatures it experiences in coastal New England...
  87. Nakashima K, Yamada L, Satou Y, Azuma J, Satoh N. The evolutionary origin of animal cellulose synthase. Dev Genes Evol. 2004;214:81-8 pubmed publisher
    ..Here we report a Ciona intestinalis homolog of cellulose synthase, which is the core catalytic subunit of multi-enzyme complexes where cellulose ..
  88. Shi W, Peyrot S, Munro E, Levine M. FGF3 in the floor plate directs notochord convergent extension in the Ciona tadpole. Development. 2009;136:23-8 pubmed publisher
    ..However, it is not known what signals coordinate CE movements across cell fields. In the simple chordate Ciona intestinalis, the notochord plate consists of just 40 cells, which undergo mediolateral convergence (intercalation) to ..
  89. Gaffney J, Valentine A. Contrasting synergistic anion effects in vanadium(V) binding to nicatransferrin versus human serum transferrin. Biochemistry. 2009;48:11609-11 pubmed publisher
    ..The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma...
  90. Thompson J, Di Gregorio A. Insulin-like genes in ascidians: findings in Ciona and hypotheses on the evolutionary origins of the pancreas. Genesis. 2015;53:82-104 pubmed publisher
    ..Here we show that two of the three insulin-like genes identified in Ciona intestinalis, an invertebrate chordate with a dual life cycle, are first expressed in the developing notochord during ..
  91. Hozumi A, Horie T, Sasakura Y. Neuronal map reveals the highly regionalized pattern of the juvenile central nervous system of the ascidian Ciona intestinalis. Dev Dyn. 2015;244:1375-93 pubmed publisher
    ..and position of differentiated neurons, and their axonal trajectories in the juvenile CNS of the ascidian Ciona intestinalis. The cell bodies of cholinergic, glutamatergic, and GABAergic/glycinergic neurons exhibited different ..
  92. Kelly R. Adhesive Enrichment and Membrane Turnover at the Heart of Cardiopharyngeal Induction. Dev Cell. 2015;34:490-2 pubmed publisher
    ..Differential inductive signaling during asymmetric division of progenitor cells specifies the heart lineage in Ciona intestinalis. In this issue of Developmental Cell, Cota and Davidson (2015) show that differential induction is mediated ..