Chinese hamster

Summary

Alias: Chinese hamsters, Cricetulus griseus, Cricetulus aureus, Cricetulus barabensis griseus, Cricetus griseus, CHO cell lines, Cricetulus griseus (Milne-Edwards, 1867)

Top Publications

  1. Heine H, Kirschning C, Lien E, Monks B, Rothe M, Golenbock D. Cutting edge: cells that carry A null allele for toll-like receptor 2 are capable of responding to endotoxin. J Immunol. 1999;162:6971-5 pubmed
    ..CD14-transfected Chinese hamster ovary (CHO)-K1 fibroblasts (CHO/CD14) are exquisitely sensitive to endotoxin...
  2. Marin M, Tailor C, Nouri A, Kozak S, Kabat D. Polymorphisms of the cell surface receptor control mouse susceptibilities to xenotropic and polytropic leukemia viruses. J Virol. 1999;73:9362-8 pubmed
    ..Furthermore, expression of an X-MLV envelope glycoprotein in Chinese hamster ovary cells interfered efficiently with X-MLV and P-MLV infections mediated by X-receptors that contained ..
  3. Lien E, Means T, Heine H, Yoshimura A, Kusumoto S, Fukase K, et al. Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide. J Clin Invest. 2000;105:497-504 pubmed
    ..RSLA and lipid IVa strongly induced NF-kappaB activity and IL-6 release in Chinese hamster ovary fibroblasts expressing CD14 (CHO/CD14), but these compounds antagonized LPS antagonists in CHO/CD14 ..
  4. Sugaya K, Hongo E, Ishihara Y, Tsuji H. The conserved role of Smu1 in splicing is characterized in its mammalian temperature-sensitive mutant. J Cell Sci. 2006;119:4944-51 pubmed
    ..Comparison of the results of perlecan RT-PCR analysis with those of analysis of four other genes suggested that the splicing defect in the perlecan gene is unique and that it is conserved through evolution. ..
  5. Kuge O, Dascher C, Orci L, Rowe T, Amherdt M, Plutner H, et al. Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments. J Cell Biol. 1994;125:51-65 pubmed
  6. Nagahama M, Orci L, Ravazzola M, Amherdt M, Lacomis L, Tempst P, et al. A v-SNARE implicated in intra-Golgi transport. J Cell Biol. 1996;133:507-16 pubmed
    ..Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles. ..
  7. Habuchi H, Kobayashi M, Kimata K. Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells. J Biol Chem. 1998;273:9208-13 pubmed
    ..The enzyme was purified to apparent homogeneity from the serum-free culture medium of Chinese hamster ovary (CHO) cells (Habuchi, H., Habuchi, O., and Kimata, K. (1995) J. Biol Chem. 270, 4172-4179)...
  8. Heine H, Delude R, Monks B, Espevik T, Golenbock D. Bacterial lipopolysaccharide induces expression of the stress response genes hop and H411. J Biol Chem. 1999;274:21049-55 pubmed
    CD14-transfected Chinese hamster ovary K1 fibroblasts (CHO/CD14) respond to lipopolysaccharide (LPS) by metabolizing arachidonic acid and with translocation of NF-kappaB to the nucleus...
  9. Alexandrow M, Hamlin J. Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A. Mol Cell Biol. 2004;24:1614-27 pubmed
    ..To resolve the questions regarding regulation of endogenous Cdc6, we cloned the cDNA encoding the Chinese hamster Cdc6 homolog and specifically focused on analyzing the localizations and chromatin affinities of endogenous ..

More Information

Publications407 found, 100 shown here

  1. Radhakrishnan A, Sun L, Kwon H, Brown M, Goldstein J. Direct binding of cholesterol to the purified membrane region of SCAP: mechanism for a sterol-sensing domain. Mol Cell. 2004;15:259-68 pubmed
    ..The current studies show that cells control their cholesterol content through receptor-ligand interactions and not through changes in the physical properties of the membrane. ..
  2. Sugaya K, Ishihara Y, Sugaya K. Enlargement of speckles of SF2/ASF due to loss of function of Smu1 is characterized in the mammalian temperature-sensitive mutant. RNA Biol. 2011;8:488-95 pubmed
    ..The significant decrease of Smu1 may lead the enlargement of speckles of SF2/ASF. These data show the importance of Smu1 as a regulator of splicing and genome maintenance. ..
  3. Guo Q, Vasile E, Krieger M. Disruptions in Golgi structure and membrane traffic in a conditional lethal mammalian cell mutant are corrected by epsilon-COP. J Cell Biol. 1994;125:1213-24 pubmed
    ..Thus, along with biochemical and yeast genetics methods, mammalian somatic cell mutants can provide powerful tools for the elucidation of the mechanisms underlying intracellular membrane traffic. ..
  4. Sugaya K, Sasanuma S, Nohata J, Kimura T, Hongo E, Higashi T, et al. Cloning and sequencing for the largest subunit of Chinese hamster RNA polymerase II gene: identification of a mutation related to abnormal induction of sister chromatid exchanges. Gene. 1997;194:267-72 pubmed
    ..induction of sister chromatid exchanges (SCEs) in the RNA polymerase II largest subunit (RpII LS) gene of the Chinese hamster CHO-KI cell mutant, we have completely sequenced the whole region of the RpII LS cDNAs obtained from normal ..
  5. Sugaya K, Hongo E, Tsuji H. A temperature-sensitive mutation in the WD repeat-containing protein Smu1 is related to maintenance of chromosome integrity. Exp Cell Res. 2005;306:242-51 pubmed
    ..Thus, Smu1 appears to be involved directly or indirectly in DNA replication, activation of cdc2 kinase, spindle assembly, and maintenance of chromosome integrity, reflecting the important roles of Smu1 in cellular function. ..
  6. Aridor M, Fish K, Bannykh S, Weissman J, Roberts T, Lippincott Schwartz J, et al. The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly. J Cell Biol. 2001;152:213-29 pubmed
    ..Our results demonstrate an unanticipated and novel role for Sar1 in linking cargo selection with ER morphogenesis through the generation of transitional tubular ER export sites...
  7. Wang X, Cheng Y, Xue H, Yue Y, Zhang W, Li X. Fargesin as a potential β₁ adrenergic receptor antagonist protects the hearts against ischemia/reperfusion injury in rats via attenuating oxidative stress and apoptosis. Fitoterapia. 2015;105:16-25 pubmed publisher
    ..of fargesin on isoproterenol-(ISO-) induced cells injury in the high expression β1 adrenergic receptor/Chinese hamster ovary-S (β1AR/CHO-S) cells and occluding the left coronary artery- (LAD-) induced myocardial ischemia/..
  8. Gombos I, Crul T, Piotto S, Güngör B, Török Z, Balogh G, et al. Membrane-lipid therapy in operation: the HSP co-inducer BGP-15 activates stress signal transduction pathways by remodeling plasma membrane rafts. PLoS ONE. 2011;6:e28818 pubmed publisher
    ..raft marker glycosylphosphatidylinositol-anchored monomeric green fluorescent protein diffusing in the live Chinese hamster ovary (CHO) cell plasma membrane demonstrated that BGP-15 prevents the transient structural disintegration of ..
  9. Zhang M, Yin H, Wang W, Li J, Wang X. Over-expressed human TREK-1 inhibits CHO cell proliferation via inhibiting PKA and p38 MAPK pathways and subsequently inducing G1 arrest. Acta Pharmacol Sin. 2016;37:1190-8 pubmed publisher
    ..In this study, we investigated how TREK-1 affected the proliferation of Chinese hamster ovary (CHO) cells in vitro. A CHO cell line stably expressing hTREK-1 (CHO/hTREK-1 cells) was generated...
  10. Deshmukh A, Bai S, T A, Kazi R, Banarjee R, Rathore R, et al. Methylglyoxal attenuates insulin signaling and downregulates the enzymes involved in cholesterol biosynthesis. Mol Biosyst. 2017;13:2338-2349 pubmed publisher
    ..By using insulin sensitive rat muscle cells (L6) and Chinese hamster ovary (CHO) cells stably expressing the insulin receptor (IR) and a glucose transporter fused with green ..
  11. Wang M, Shan J, Yang Q, Ma X, Jin S, Guo X, et al. Antiarrhythmic efficacy of CPUY102122, a multiple ion channel blocker, on rabbits with ischemia/reperfusion injury. Pharmacol Rep. 2014;66:1022-30 pubmed publisher
    ..delayed rectifier potassium channel current (IKr) was studied using whole-cell patch clamp techniques in Chinese Hamster Ovary cells stably expressing human Ether-à-go-go-Related Gene...
  12. Potluri P, Procaccio V, Scheffler I, Wallace D. High throughput gene complementation screening permits identification of a mammalian mitochondrial protein synthesis (ρ(-)) mutant. Biochim Biophys Acta. 2016;1857:1336-1343 pubmed publisher
    ..We have used this system to transduce the Chinese hamster V79-G7 OXPHOS mutant cell line with a defect in mitochondrial protein synthesis...
  13. Marchal E, Schellens S, Monjon E, Bruyninckx E, Marco H, Gäde G, et al. Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors. Int J Mol Sci. 2018;19: pubmed publisher
    ..including the respective endogenous AKHs, at these receptors was tested with a bioluminescence-based assay in Chinese hamster ovary cells. All receptors were activated by their endogenous ligand in the nanomolar range...
  14. Sun M, Mu Y, Ding Y, Ao J, Chen X. Molecular and functional characterization of Toll-like receptor 21 in large yellow croaker (Larimichthys crocea). Fish Shellfish Immunol. 2016;59:179-188 pubmed publisher
    ..4 (CD4) and the transmembrane and cytoplasmic domains of LycTLR21 could activate NF-?B, but not IFN-? in Chinese hamster ovary (CHO) cells, suggesting that LycTLR21 could mediate activation of NF-?B...
  15. Ganesan S, Nteeba J, Keating A. Enhanced susceptibility of ovaries from obese mice to 7,12-dimethylbenz[a]anthracene-induced DNA damage. Toxicol Appl Pharmacol. 2014;281:203-10 pubmed publisher
    ..isolated and abundance of Ataxia telangiectasia mutated (Atm), X-ray repair complementing defective repair in Chinese hamster cells 6 (Xrcc6), breast cancer type 1 (Brca1), Rad 51 homolog (Rad51), poly [ADP-ribose] polymerase 1 (Parp1) ..
  16. Ashbrook A, Burrack K, Silva L, Montgomery S, Heise M, Morrison T, et al. Residue 82 of the Chikungunya virus E2 attachment protein modulates viral dissemination and arthritis in mice. J Virol. 2014;88:12180-92 pubmed publisher
    ..Using wild-type and glycosaminoglycan (GAG)-deficient Chinese hamster ovary (CHO) cell lines and soluble GAGs, we found that an arginine at residue 82 conferred greater dependence ..
  17. Otsuka R, Harada N, Aoki S, Shirai K, Nishitsuji K, Nozaki A, et al. C-terminal region of GADD34 regulates eIF2α dephosphorylation and cell proliferation in CHO-K1 cells. Cell Stress Chaperones. 2016;21:29-40 pubmed publisher
    ..GADD34 is a member of a growth arrest and DNA damage (GADD)-inducible gene family. Here, we established a novel Chinese hamster ovary (CHO)-K1-derived cell line, CHO-K1-G34M, which carries a nonsense mutation (termed the Q525X mutation) ..
  18. Tanaka A, Tumkosit U, Nakamura S, Motooka D, Kishishita N, Priengprom T, et al. Genome-Wide Screening Uncovers the Significance of N-Sulfation of Heparan Sulfate as a Host Cell Factor for Chikungunya Virus Infection. J Virol. 2017;91: pubmed publisher
    ..We show, by comparing previous reports using Chinese hamster ovary cells, along with another observation that enhanced infectivity of CHIKV bearing Arg82 in envelope E2 ..
  19. Hou Q, Chen E, Jiang H, Wei D, Gui S, Wang J, et al. Adipokinetic hormone receptor gene identification and its role in triacylglycerol mobilization and sexual behavior in the oriental fruit fly (Bactrocera dorsalis). Insect Biochem Mol Biol. 2017;90:1-13 pubmed publisher
    ..When expressed in Chinese hamster ovary (CHO) cells, Bacdo-AKHR exhibited a high sensitivity and selectivity for AKH peptide (EC50Â..
  20. Bailon Moscoso N, Tinitana F, Martínez Espinosa R, Jaramillo Velez A, Palacio Arpi A, Aguilar Hernandez J, et al. Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador. BMC Complement Altern Med. 2017;17:539 pubmed publisher
    ..D-384), prostate cancer (PC-3), breast cancer (MCF-7), colon cancer (RKO), lung cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay...
  21. Lopez Perez M, Larsen M, Bayarri Olmos R, Ampomah P, Stevenson L, Arevalo Herrera M, et al. IgG Responses to the Plasmodium falciparum Antigen VAR2CSA in Colombia Are Restricted to Pregnancy and Are Not Induced by Exposure to Plasmodium vivax. Infect Immun. 2018;86: pubmed publisher
    ..In contrast, IgG reactivity to a homologous full-length VAR2CSA-type protein expressed in Chinese hamster ovary (CHO) cells was low and infrequent among the Colombian plasma samples, as was reactivity to both ..
  22. Hwang S, Kruzel M, Actor J. CHO expressed recombinant human lactoferrin as an adjuvant for BCG. Int J Immunopathol Pharmacol. 2015;28:452-68 pubmed publisher
    ..This study examines a Chinese hamster ovary (CHO) expressed recombinant human LF (rHLF) to boost efficacy of the BCG vaccine and delay early ..
  23. Hu J, Liu M, Hou C, She Z, Wang D, Hao S, et al. Gene expression pattern of KIFC3 during spermatogenesis of the skink Eumeces chinensis. Gene. 2015;556:206-12 pubmed publisher
    ..2%, 67.8%, 68.8%, 69.3% and 76.8% identity with its homologues in Xenopus laevis, Mus musculus, Cricetulus griseus, Homo sapiens, and Gallus gallus...
  24. Pronin A, Wang Q, Slepak V. Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor. Mol Pharmacol. 2017;92:601-612 pubmed publisher
    ..the insulinotropic effect of Oxo-M and CCh-induced Ca2+ upregulation; however, in HEK293T or Chinese hamster ovary-K1 cells overexpressing M3R, pilocarpine induces Ca2+ transients like those recorded with ..
  25. Jeon J, Lee H, Cho M, Park O, Park J, Rhie G. The Poly-γ-d-Glutamic Acid Capsule Surrogate of the Bacillus anthracis Capsule Is a Novel Toll-Like Receptor 2 Agonist. Infect Immun. 2015;83:3847-56 pubmed publisher
    ..PGA stimulated Toll-like receptor 2 (TLR2) but not TLR4 in Chinese hamster ovary cells expressing either TLR2 or TLR4...
  26. Thibonnier M, Conarty D, Plesnicher C. Mediators of the mitogenic action of human V(1) vascular vasopressin receptors. Am J Physiol Heart Circ Physiol. 2000;279:H2529-39 pubmed
    ..To explore the intracellular mediators of the mitogenic action of V(1)R, Chinese hamster ovary (CHO) cells were stably transfected with the human V(1)R cDNA clone we isolated previously...
  27. Terada T, Sugihara Y, Nakamura K, Sato R, Inazu N, Maeda M. Cloning and bacterial expression of monomeric short-chain dehydrogenase/reductase (carbonyl reductase) from CHO-K1 cells. Eur J Biochem. 2000;267:6849-57 pubmed
    ..rCHO-CR had broad substrate specificity towards xenobiotic carbonyl compounds. RT-PCR of Chinese hamster tissues suggest that CHO-CR is highly expressed in kidney, testis, brain, heart, liver, uterus and ovary...
  28. Gaughan C. The present state of the art in expression, production and characterization of monoclonal antibodies. Mol Divers. 2016;20:255-70 pubmed publisher
    ..this review, the present state of the science that underlies the development of new antibodies therapies in Chinese hamster ovary cells combined with a description of the present challenges facing the industry in terms of the ..
  29. Chen R. The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits. Microb Cell Fact. 2015;14:197 pubmed publisher
    ..described how improving erythropoietin (EPO) glycosylation can be achieved via metabolic engineering of Chinese hamster ovary (CHO) cells...
  30. Gao Z, Zhang H, Hu F, Yang L, Yang X, Zhu Y, et al. Glycan-deficient PrP stimulates VEGFR2 signaling via glycosaminoglycan. Cell Signal. 2016;28:652-62 pubmed publisher
    Whether the two N-linked glycans are important in prion, PrP, biology is unresolved. In Chinese hamster ovary (CHO) cells, the two glycans are clearly not important in the cell surface expression of transfected human PrP...
  31. Römermann K, Fedrowitz M, Hampel P, Kaczmarek E, Töllner K, Erker T, et al. Multiple blood-brain barrier transport mechanisms limit bumetanide accumulation, and therapeutic potential, in the mammalian brain. Neuropharmacology. 2017;117:182-194 pubmed publisher
    ..Furthermore, in vitro experiments with mouse organic anion transporter 3 (Oat3)-overexpressing Chinese hamster ovary cells were performed to study the interaction of bumetanide, bumetanide derivatives, and several known ..
  32. Dores R, Scuba Gray M, McNally B, Davis P, Takahashi A. Evaluating the interactions between red stingray (Dasyatis akajei) melanocortin receptors and elephant shark (Callorhinchus milii) MRAP1 and MRAP2 following stimulation with either stingray ACTH(1-24) or stingray Des-Acetyl-?MSH: A pharmacological st. Gen Comp Endocrinol. 2018;265:133-140 pubmed publisher
    ..and red stingray) do not apparently require MRAP1 for trafficking to the plasma membrane when expressed in Chinese Hamster Ovary (CHO) cells, and both orthologs can be activated by either ACTH or MSH-sized ligands...
  33. Chen I, Dixit A, Rhoads D, Roufa D. Homologous ribosomal proteins in bacteria, yeast, and humans. Proc Natl Acad Sci U S A. 1986;83:6907-11 pubmed
    ..Human ribosomal protein sequences are compared to analogous Chinese hamster, yeast, and bacterial genes...
  34. Peng H, Chang H. Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation. FEBS Lett. 1993;329:153-8 pubmed
    ..No homology was found between the eukaryotic and the prokaryotic enzymes. Northern blotting analysis revealed that the gene was expressed at the highest level in skeletal muscle, followed by liver, heart and kidney. ..
  35. Greenspan D. The carboxyl-terminal half of type VII collagen, including the non-collagenous NC-2 domain and intron/exon organization of the corresponding region of the COL7A1 gene. Hum Mol Genet. 1993;2:273-8 pubmed
    ..This region is likely to contain residues involved in the proteolytic removal of the NC-2 domain and cysteines involved in formation of the disulfide linkages which stabilize type VII collagen dimers...
  36. Wei G, Bai X, Sarkar A, Esko J. Formation of HNK-1 determinants and the glycosaminoglycan tetrasaccharide linkage region by UDP-GlcUA:Galactose beta1, 3-glucuronosyltransferases. J Biol Chem. 1999;274:7857-64 pubmed
    ..Cotransfection of the cDNA with HNK-1 3-O-sulfotransferase produced HNK-1 carbohydrate epitopes in Chinese hamster ovary (CHO) cells and COS-7 cells...
  37. Fujiwara C, Imamura A, Hashiguchi N, Shimozawa N, Suzuki Y, Kondo N, et al. Catalase-less peroxisomes. Implication in the milder forms of peroxisome biogenesis disorder. J Biol Chem. 2000;275:37271-7 pubmed
    We established a Chinese hamster ovary cell line having a temperature-sensitive phenotype in peroxisome biogenesis...
  38. Chaudhuri K, Banerjee R, Pandit B, Mukherjee A, Das S, Sengupta S, et al. Identification of two differentially expressed mitochondrial genes in a methotrexate-resistant Chinese hamster cell strain derived from v79 cells using RNA fingerprinting by arbitrary primed polymerase chain reaction. Radiat Res. 2003;160:77-85 pubmed
    ..that exhibits resistance to gamma radiation and a number of chemotherapeutic drugs compared to the parental Chinese hamster V79 cells, we used RNA fingerprinting by arbitrary primed polymerase chain reaction (RAP-PCR)...
  39. Kashino G, Hayashi K, Douhara K, Kobashigawa S, Mori H. Comparison of the biological effects of (18)F at different intracellular levels. Biochem Biophys Res Commun. 2014;454:7-11 pubmed publisher
    ..We used culture cells (Chinese Hamster Ovary cells) treated with two types of (18)F labeled drugs, fluorodeoxyglucose (FDG) and fluorine ion (HF)...
  40. Lin N, Brooks J, Sealover N, George H, Kayser K. Overexpression of Serpinb1 in Chinese hamster ovary cells increases recombinant IgG productivity. J Biotechnol. 2015;193:91-9 pubmed publisher
    ..As proof of concept, a lentiviral vector was employed to overexpress the Chinese Hamster Serpinb1 in a CHOZN(®) Glutamine Synthetase (-/-) recombinant IgG producing CHO line...
  41. Gross A, Temeyer K, Day T, Pérez de León A, Kimber M, Coats J. Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus. Insect Biochem Mol Biol. 2015;63:47-53 pubmed publisher
    ..In this study, the putative TAR1 was heterologously expressed in Chinese hamster ovary (CHO-K1) cells, and the expressed receptor resulted in a 39-fold higher potency for tyramine compared ..
  42. Fischer S, Paul A, Wagner A, Mathias S, Geiss M, Schandock F, et al. miR-2861 as novel HDAC5 inhibitor in CHO cells enhances productivity while maintaining product quality. Biotechnol Bioeng. 2015;112:2142-53 pubmed publisher
    ..Since miRNAs recently have gained much attention as next-generation cell engineering tool to improve Chinese hamster ovary (CHO) cell factories, we were interested if miRNAs are able to specifically repress HDAC expression in ..
  43. Xin L, Lin Y, Wang A, Zhu W, Liang Y, Su X, et al. Cytogenetic evaluation for the genotoxicity of bisphenol-A in Chinese hamster ovary cells. Environ Toxicol Pharmacol. 2015;40:524-9 pubmed publisher
    ..Taken together, the data obtained in this study clearly demonstrated that BPA is not mutagenic but could exhibit significant genotoxic and cytogenetic effects in Chinese hamster ovary cells.
  44. Dissanayake N, Current K, Obare S. Mutagenic Effects of Iron Oxide Nanoparticles on Biological Cells. Int J Mol Sci. 2015;16:23482-516 pubmed publisher
    ..Other cell lines including the Chinese hamster ovary cells, mouse fibroblast cells, murine fibroblast cells, Mytilus galloprovincialis sperm cells, mice ..
  45. Mascarenhas J, Korokhov N, Burger L, Kassim A, Tuter J, Miller D, et al. Genetic engineering of CHO cells for viral resistance to minute virus of mice. Biotechnol Bioeng. 2017;114:576-588 pubmed publisher
    Contamination by the parvovirus minute virus of mice (MVM) remains a challenge in Chinese hamster ovary (CHO) biopharmaceutical production processes...
  46. Migani D, Smales C, Bracewell D. Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells. Biotechnol Prog. 2017;33:666-676 pubmed publisher
    ..2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:666-676, 2017. ..
  47. Camire J, Kim D, Kwon S. Enhanced production of recombinant proteins by a small molecule protein synthesis enhancer in combination with an antioxidant in recombinant Chinese hamster ovary cells. Bioprocess Biosyst Eng. 2017;40:1049-1056 pubmed publisher
    ..carboxylic acids such as pentanoic acid and butanoic acid have been shown to promote an apoptotic response in Chinese hamster ovary (CHO) cell culture...
  48. Koechling W, Plaksin D, Croston G, Jeppesen J, Macklon K, Andersen C. Comparative pharmacology of a new recombinant FSH expressed by a human cell line. Endocr Connect. 2017;6:297-305 pubmed publisher
    ..are important therapeutic agents for the treatment of infertility, including follitropin alfa expressed in Chinese Hamster Ovary (CHO) cells and, more recently, follitropin delta expressed in the human cell line PER.C6...
  49. Hogiri T, Tamashima H, Nishizawa A, Okamoto M. Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model. J Biosci Bioeng. 2018;125:245-250 pubmed publisher
    To optimize monoclonal antibody (mAb) production in Chinese hamster ovary cell cultures, culture pH should be temporally controlled with high resolution...
  50. Fasciani I, Pietrantoni I, Rossi M, Mannoury La Cour C, Aloisi G, Marampon F, et al. Distinctive binding properties of the negative allosteric modulator, [3H]SB269,652, at recombinant dopamine D3 receptors. Eur J Pharmacol. 2018;819:181-189 pubmed publisher
    ..of radiolabelled [3H]SB269,652 to human dopamine D3 receptor stably expressed in Chinese Hamster Ovary cells. Under saturating conditions, SB269,652 showed a KD value of ? 1nM...
  51. Hamdi A, Bero J, Beaufay C, Flamini G, Marzouk Z, Vander Heyden Y, et al. In vitro antileishmanial and cytotoxicity activities of essential oils from Haplophyllum tuberculatum A. Juss leaves, stems and aerial parts. BMC Complement Altern Med. 2018;18:60 pubmed publisher
    ..m.m.) promastigotes and cytotoxicity on the Chinese Hamster Ovary (CHO) cell line. Limonene (1...
  52. Sha S, Bhatia H, Yoon S. An RNA-seq based transcriptomic investigation into the productivity and growth variants with Chinese hamster ovary cells. J Biotechnol. 2018;271:37-46 pubmed publisher
    b>Chinese hamster ovary (CHO) cells are widely used in producing monoclonal antibodies (mAbs), however, these cell lines can show variants associated with productivity and growth...
  53. Hepnarova V, Korabecny J, Matoušková L, Jost P, Muckova L, Hrabinova M, et al. The concept of hybrid molecules of tacrine and benzyl quinolone carboxylic acid (BQCA) as multifunctional agents for Alzheimer's disease. Eur J Med Chem. 2018;150:292-306 pubmed publisher
    ..receptors (mAChRs), the action of novel compounds on M1 mAChRs was evaluated via Fluo-4 NW assay on the Chinese hamster ovarian (CHO-M1WT2) cell line...
  54. Cox D, Trouillot T, Ashley P, Brabant M, Coffino P. A functional mouse ornithine decarboxylase gene (Odc) maps to chromosome 12: further evidence of homoeology between mouse chromosome 12 and the short arm of human chromosome 2. Cytogenet Cell Genet. 1988;48:92-4 pubmed
    ..a DNA probe specific for a functional mouse ornithine decarboxylase gene (Odc) in conjunction with a panel of Chinese hamster x mouse somatic cell hybrids to assign Odc to mouse chromosome 12...
  55. Kawanabe Y, Okamoto Y, Enoki T, Hashimoto N, Masaki T. Ca(2+) channels activated by endothelin-1 in CHO cells expressing endothelin-A or endothelin-B receptors. Am J Physiol Cell Physiol. 2001;281:C1676-85 pubmed
    We compared the Ca(2+) channels activated by endothelin-1 (ET-1) in Chinese hamster ovary (CHO) cells stably expressing endothelin type A (ET(A)) or endothelin type B (ET(B)) receptors using the Ca(2+) channel blockers LOE-908 and SK&..
  56. Wiegant W, Overmeer R, Godthelp B, van Buul P, Zdzienicka M. Chinese hamster cell mutant, V-C8, a model for analysis of Brca2 function. Mutat Res. 2006;600:79-88 pubmed publisher
    The previously described Chinese hamster cell mutant V-C8 that is defective in Brca2 shows a very complex phenotype, including increased sensitivity towards a wide variety of DNA damaging agents, chromosomal instability, abnormal ..
  57. Golani L, George C, Zhao S, Raghavan S, Orr S, Wallace A, et al. Structure-activity profiles of novel 6-substituted pyrrolo[2,3-d]pyrimidine thienoyl antifolates with modified amino acids for cellular uptake by folate receptors α and β and the proton-coupled folate transporter. J Med Chem. 2014;57:8152-66 pubmed publisher
    ..Compounds 7 and 10-13 were selectively inhibitory toward folate receptor (FR) α-expressing Chinese hamster ovary (CHO) cells...
  58. Matsuyama R, Tsutsui T, Lee K, Onitsuka M, Omasa T. Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells. J Biosci Bioeng. 2015;120:701-8 pubmed publisher
    The dihydrofolate reductase gene amplification system is widely used in Chinese hamster ovary (CHO) cells for the industrial production of therapeutic proteins...
  59. Stiefel F, Paul A, Jacopo T, Sgueglia A, Stützle M, Herold E, et al. The influence of bisphenol A on mammalian cell cultivation. Appl Microbiol Biotechnol. 2016;100:113-24 pubmed publisher
    ..however, it influenced the specific productivity rate and metabolic activity parameters of our Chinese hamster ovary (CHO) cell line...
  60. Liu K, Jiang Y. Polymorphisms in DNA Repair Gene and Susceptibility to Glioma: A Systematic Review and Meta-Analysis Based on 33 Studies with 15 SNPs in 9 Genes. Cell Mol Neurobiol. 2017;37:263-274 pubmed publisher
    ..susceptibility is associated with rs1799782 and rs25487 of X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1), rs1805377 of XRCC4, rs1800067 of excision repair cross-complementing rodent repair ..
  61. Zhu B, Cheng D, Li S, Zhou S, Yang Q. High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the ?-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis. Int J Mol Sci. 2016;17: pubmed publisher
    Increasing evidences show that XRCC6 (X-ray repair complementing defective repair in Chinese hamster cells 6) was upregulated and involved in tumor growth in several tumor types...
  62. Hoeke G, Nahon K, Bakker L, Norkauer S, Dinnes D, Kockx M, et al. Short-term cooling increases serum triglycerides and small high-density lipoprotein levels in humans. J Clin Lipidol. 2017;11:920-928.e2 pubmed publisher
    ..cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. Short-term cooling increased serum levels of free fatty acids, triglycerides, and cholesterol...
  63. Chung C, Wang Q, Yang S, Ponce S, Kirsch B, Zhang H, et al. Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells. Biotechnol Bioeng. 2017;114:2848-2856 pubmed publisher
    ..In this study, the disruption of two ?-2,3 sialyltransferases (ST3GAL4 and ST3GAL6) from Chinese Hamster Ovary (CHO) cells was combined with protein engineering of the Fc region to generate an IgG containing 80% ..
  64. Sarıgöl Kılıç Z, Aydin S, Ündeğer Bucurgat Ü, Basaran N. In vitro genotoxicity assessment of dinitroaniline herbicides pendimethalin and trifluralin. Food Chem Toxicol. 2018;113:90-98 pubmed publisher
    ..species (ROS) levels were measured to examine the potential of both compounds to induce oxidative damage in Chinese hamster lung fibroblast (V79) cells...
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    ..This study provides a picture of the domain structure of SREBP-1 and further elucidates the mechanism by which it adjusts gene transcription to maintain cholesterol homeostasis in animal cells...
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    ..This farnesylated protein, designated PxF, is localized to the outer surface of peroxisomes as determined by indirect immunofluorescence and electron microscopy...
  67. Eckhardt M, Gerardy Schahn R. Molecular cloning of the hamster CMP-sialic acid transporter. Eur J Biochem. 1997;248:187-92 pubmed
    b>Chinese hamster ovary (CHO) glycosylation mutants of the Lec2 complementation group are unable to express sialylated glycoproteins and glycolipids due to a defect in the Golgi CMP-sialic acid transporter (CMP-Sia-Tr)...
  68. Fowler B, Mackman N, Parmer R, Miles L. Binding of human single chain urokinase to Chinese Hamster Ovary cells and cloning of hamster u-PAR. Thromb Haemost. 1998;80:148-54 pubmed
    ..b>Chinese Hamster Ovary (CHO) cells bound human scu-PA with high affinity and capacity (Kd = 1.13 +/- 0.8 nM; Bmax = 5.45 +/- 0...
  69. McCloskey D, Pegg A. Altered spermidine/spermine N1-acetyltransferase activity as a mechanism of cellular resistance to bis(ethyl)polyamine analogues. J Biol Chem. 2000;275:28708-14 pubmed
    ..periods in drug-free medium were used to select an N(1), N(12)-bis(ethyl)spermine-resistant derivative of the Chinese hamster ovary cell line C55.7. The resulting C55...
  70. Nakamura T, Omasa T. Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system. J Biosci Bioeng. 2015;120:323-9 pubmed publisher
    Therapeutic antibodies are commonly produced by high-expressing, clonal and recombinant Chinese hamster ovary (CHO) cell lines...
  71. Ji R, Qian Y, Hu A, Hu Y. DNA repair gene XRCC3 T241M polymorphism and susceptibility to hepatocellular carcinoma in a Chinese population: a meta-analysis. Genet Mol Res. 2015;14:15988-96 pubmed publisher
    Numerous studies have evaluated the association between the X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3) T241M polymorphism and hepatocellular carcinoma (HCC) risk...
  72. Gillespie L, Roosendahl P, Ng W, Brooks A, Reading P, Londrigan S. Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN. Sci Rep. 2016;6:19428 pubmed publisher
    ..Sialic acid-deficient Lec2 Chinese Hamster Ovary (CHO) cell lines were resistant to IAV infection whereas expression of DC-SIGN/L-SIGN restored ..
  73. Xu D, Hu X. Photoperiod and temperature differently affect immune function in striped hamsters (Cricetulus barabensis). Comp Biochem Physiol A Mol Integr Physiol. 2017;204:211-218 pubmed publisher
    ..Cold stress reduced humoral immunity and WBC, which might account for the highest mortality in winter in this species. Blood glucose, leptin and corticosterone could not interpret the changes of immunity in hamsters. ..
  74. Heffner K, Wang Q, Hizal D, Can Ã, Betenbaugh M. Glycoengineering of Mammalian Expression Systems on a Cellular Level. Adv Biochem Eng Biotechnol. 2018;: pubmed publisher
    Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein ..
  75. Gurung R, Kim D, Kim L, Lee A, Wang Z, Gao Y. Toxicological evaluation of 6'-sialyllactose (6'-SL) sodium salt. Regul Toxicol Pharmacol. 2018;95:182-189 pubmed publisher
    ..A chromosomal aberration assay (using Chinese hamster lung cells) found no clastogenic effects at any concentration of 6'-SL sodium salt in the presence or absence ..
  76. Hung S, Yu C, Chou T, Huang P, Lin L. Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region. Biochim Biophys Acta. 1991;1090:255-8 pubmed
    A genomic DNA clone containing Chinese hamster metallothionein II (MTII) gene and its 5' flanking region was isolated from Cd resistant Chinese hamster ovary (CHO) cells...
  77. Ahmad S, Gupta R. Cloning of a Chinese hamster protein homologous to the mouse t-complex protein TCP-1: structural similarity to the ubiquitous 'chaperonin' family of heat-shock proteins. Biochim Biophys Acta. 1990;1087:253-5 pubmed
    The complete cDNA sequence of a Chinese hamster ovary cell protein, homologous to a mouse t-complex protein, TCP-1, has been determined...
  78. Joyal J, Crimmins D, Thoma R, Sacks D. Identification of insulin-stimulated phosphorylation sites on calmodulin. Biochemistry. 1996;35:6267-75 pubmed publisher
    ..To determine the insulin-sensitive phosphorylation sites, phosphocalmodulin was immunoprecipitated from Chinese hamster ovary cells expressing human insulin receptors (CHO/IR)...
  79. Kuge O, Saito K, Nishijima M. Cloning of a Chinese hamster ovary (CHO) cDNA encoding phosphatidylserine synthase (PSS) II, overexpression of which suppresses the phosphatidylserine biosynthetic defect of a PSS I-lacking mutant of CHO-K1 cells. J Biol Chem. 1997;272:19133-9 pubmed
    ..We previously showed the presence of two different enzymes catalyzing the serine base exchange in Chinese hamster ovary (CHO) cells and isolated the cDNA of one of the enzymes, PtdSer synthase (PSS) I, which also catalyzes ..
  80. Ye Q, Hyndman D, Li X, Flynn T, Jia Z. Crystal structure of CHO reductase, a member of the aldo-keto reductase superfamily. Proteins. 2000;38:41-8 pubmed
    b>Chinese hamster ovary (CHO) reductase is an enzyme belonging to the aldo-keto reductase (AKR) superfamily that is induced by the aldehyde-containing protease inhibitor ALLN (Inoue, Sharma, Schimke, et al., J Biol Chem 1993;268: 5894)...
  81. Espenshade P, Li W, Yabe D. Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER. Proc Natl Acad Sci U S A. 2002;99:11694-9 pubmed publisher
    ..These studies demonstrate feedback control of a biosynthetic pathway by the regulated binding of COPII proteins to an endoplasmic reticulum-to-Golgi transport protein...
  82. Chen W, Stanley P. Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase I. Glycobiology. 2003;13:43-50 pubmed publisher
    ..These Lec1 mutants provide a novel cohort for investigating the effects on Golgi trafficking and kin recognition of deletion mutants of GlcNAc-TI expressed at endogenous rather than nonphysiological levels...
  83. Takahashi M, Murate M, Fukuda M, Sato S, Ohta A, Kobayashi T. Cholesterol controls lipid endocytosis through Rab11. Mol Biol Cell. 2007;18:2667-77 pubmed
    Cellular cholesterol increases when cells reach confluency in Chinese hamster ovary (CHO) cells. We examined the endocytosis of several lipid probes in subconfluent and confluent CHO cells...
  84. Druz A, Chu C, Majors B, Santuary R, Betenbaugh M, Shiloach J. A novel microRNA mmu-miR-466h affects apoptosis regulation in mammalian cells. Biotechnol Bioeng. 2011;108:1651-61 pubmed publisher
    This study determined the changes in microRNA (miRs) expression in mammalian Chinese hamster ovary (CHO) cells undergoing apoptosis induced by exposing the cells to nutrient-depleted media...
  85. Hayashi S, Amari M, Okamoto K. Loss of calretinin- and parvalbumin-immunoreactive axons in anterolateral columns beyond the corticospinal tracts of amyotrophic lateral sclerosis spinal cords. J Neurol Sci. 2013;331:61-6 pubmed publisher
    ..These findings suggest that a loss of CaBP-ir axons may precede the development of dMP in ALS patients' spinal cords, and the dying back mechanism would underlie this phenomenon. ..
  86. Fomina Yadlin D, Du Z, McGrew J. Gene expression measurements normalized to cell number reveal large scale differences due to cell size changes, transcriptional amplification and transcriptional repression in CHO cells. J Biotechnol. 2014;189:58-69 pubmed publisher
    ..applied because total RNA yield from a set number of cells varies among experimental treatments of the same Chinese Hamster Ovary (CHO) cell line and among different CHO cell lines expressing recombinant proteins...
  87. Ferreira Camargo L, Tran M, Beld J, Burkart M, Mayfield S. Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae. AMB Express. 2015;5:126 pubmed publisher
    ..In other expression systems, as Escherichia coli, Chinese hamster ovary cells, and insect cells, recombinant protein accumulation can be hampered by cell's inability to fold ..
  88. Yin B, Gao Y, Chung C, Yang S, Blake E, Stuczynski M, et al. Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation. Biotechnol Bioeng. 2015;112:2343-51 pubmed publisher
    ..of recombinant erythropoietin (rEPO), a human α2,6-sialyltransferase (ST6Gal1) was expressed in Chinese hamster ovary (CHO-K1) cells...
  89. Jukić S, Bubenik D, Pavlović N, Tušek A, Srček V. Adaptation of CHO cells in serum-free conditions for erythropoietin production: Application of EVOP technique for process optimization. Biotechnol Appl Biochem. 2016;63:633-641 pubmed publisher
    ..In the present study, a Chinese hamster ovary (CHO) cell line producing glycosylated recombinant human erythropoietin (rhEPO) was adapted for growth ..
  90. Kunert R, Reinhart D. Advances in recombinant antibody manufacturing. Appl Microbiol Biotechnol. 2016;100:3451-61 pubmed publisher
    Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression, production processes have steadily improved through numerous advances...
  91. Li Y, Wang X, Yu J, Shao F, Zhang Y, Lu X, et al. MiR-122 targets the vanin 1 gene to regulate its expression in chickens. Poult Sci. 2016;95:1145-50 pubmed publisher
    ..of miR-122 decreased the expression of luciferase reporter gene linked to the 3'UTR of chicken VNN1 in the Chinese hamster ovary cells (P<0...
  92. Passos K, Fiorini A, Rosado F, Freitas D, Lima Neto Q, Pattaro Júnior J, et al. Ability of HMGB1 protein to bind to intrinsically bent and non-bent DNA sites in the AMPD2 gene amplicon. Genet Mol Res. 2016;15: pubmed publisher
    ..All fragments showed efficient binding to HMGB1. However, the non-bent DNA fragments nb2, nb4, and nb5 showed slightly reduced binding efficiency. ..