Trypanosoma cruzi strain CL Brener



Top Publications

  1. Droll D, Archer S, Fenn K, Delhi P, Matthews K, Clayton C. The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation. FEBS Lett. 2010;584:1156-62 pubmed publisher
    ..We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing. ..
  2. Picchi G, Ferreira A, Souza F, Lourenço E, Arauco P, Lorusso A, et al. Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1. Exp Parasitol. 2011;127:147-52 pubmed publisher
    ..We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope. ..
  3. Campos P, Silva V, Furtado C, Machado Silva A, DaRocha W, Peloso E, et al. Trypanosoma cruzi MSH2: Functional analyses on different parasite strains provide evidences for a role on the oxidative stress response. Mol Biochem Parasitol. 2011;176:8-16 pubmed publisher
    ..More importantly, they also indicate that, in addition to its role in MMR, TcMSH2 acts in the parasite response to oxidative stress through a novel mitochondrial function that may be conserved in T. brucei. ..
  4. Gluenz E, Taylor M, Kelly J. The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. Int J Parasitol. 2007;37:617-25 pubmed
    ..To investigate the function of Met-III, we used gene deletion. This showed that Met-III is not required for the development of metacyclic trypomastigotes and that null mutants can complete the life-cycle in vitro. ..
  5. Coelho E, Rodrigues D, Urmenyi T, Rondinelli E, Silva R. Polymorphic and differential expression of the Trypanosoma cruzi alleles containing universal minicircle binding protein. Biochem Biophys Res Commun. 2006;341:382-90 pubmed
  6. Woelke M, Paulucci N, Selva A, Garban H, de Lema M. ?9 desaturase from Trypanosoma cruzi: Key enzyme in the parasite metabolism. Cloning and overexpression. Microbiol Res. 2017;194:29-37 pubmed publisher
    ..Since Tc?9DES has shown active participation under different environmental factors, among them, ketoconazole, we consider that it plays a critical role in the metabolism of the parasite. ..
  7. Leroux A, Maugeri D, Cazzulo J, Nowicki C. Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi. Mol Biochem Parasitol. 2011;177:61-4 pubmed publisher
    ..This observation fits in well with the expected oxidative burst this pathogen has to face when infecting the mammalian host. ..
  8. Galizzi M, Bustamante J, Fang J, Miranda K, Soares Medeiros L, Tarleton R, et al. Evidence for the role of vacuolar soluble pyrophosphatase and inorganic polyphosphate in Trypanosoma cruzi persistence. Mol Microbiol. 2013;90:699-715 pubmed publisher
  9. Mathieu Daudé F, Lafay B, Touzet O, Lelièvre J, Parrado F, Bosseno M, et al. Exploring the FL-160-CRP gene family through sequence variability of the complement regulatory protein (CRP) expressed by the trypomastigote stage of Trypanosoma cruzi. Infect Genet Evol. 2008;8:258-66 pubmed publisher
    ..This work provides also information for the T. cruzi genome annotation of the trans-sialidase-like putative proteins. ..

More Information


  1. Schamber Reis B, Nardelli S, Régis Silva C, Campos P, Cerqueira P, Lima S, et al. DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions. Mol Biochem Parasitol. 2012;183:122-31 pubmed publisher
    ..Taken together our data suggest participation of Tcpol? during kDNA replication and repair of oxidative DNA damage induced by genotoxic stress in this organelle. ..
  2. Venegas J, Aslund L, Solari A. Cloning and characterization of a DNA polymerase beta gene from Trypanosoma cruzi. Parasitol Int. 2009;58:187-92 pubmed publisher
  3. Pavani R, Da Silva M, Fernandes C, Morini F, Araujo C, Fontes M, et al. Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi. PLoS Negl Trop Dis. 2016;10:e0005181 pubmed publisher
    ..Taken together, these findings indicate the involvement of TcRPA in the metacyclogenesis process and suggest that a delay in cell cycle progression could be linked with differentiation in T. cruzi. ..
  4. López Castilla A, Pons T, Pires J. NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi. J Struct Biol. 2015;190:11-20 pubmed publisher
    ..Q4D059 adopts a α/β fold that is slightly similar to the ATPase sub-domain IIB of the heat-shock protein 70 (HSP70) and to the N-terminal domain of the ribosomal protein L11. ..
  5. Maeda F, Cortez C, Izidoro M, Juliano L, Yoshida N. Fibronectin-degrading activity of Trypanosoma cruzi cysteine proteinase plays a role in host cell invasion. Infect Immun. 2014;82:5166-74 pubmed publisher
    ..Overall, our results have indicated that the cysteine proteinase of T. cruzi metacyclic forms, through its fibronectin-degrading activity, is implicated in host cell invasion. ..
  6. Kalb L, Frederico Y, Batista C, Eger I, Fragoso S, Soares M. Clathrin expression in Trypanosoma cruzi. BMC Cell Biol. 2014;15:23 pubmed publisher
    ..Our data also indicate that in T. cruzi epimastigotes clathrin-mediated endocytosis of albumin occurs at the flagellar pocket, while clathrin-independent endocytosis of transferrin occurs at the cytostome/cytopharynx complex. ..
  7. Moen S, Fairman J, Barnes S, Sullivan A, Nakazawa Hewitt S, Van Voorhis W, et al. Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP. Acta Crystallogr F Struct Biol Commun. 2015;71:609-14 pubmed publisher
    ..major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target. ..
  8. Oliveira I, Gonçalves A, Neves J, von Itzstein M, Todeschini A. Evidence of ternary complex formation in Trypanosoma cruzi trans-sialidase catalysis. J Biol Chem. 2014;289:423-36 pubmed publisher
    ..In summary, our data put forward a coherent dynamic framework to understand how a glycosidase evolved its highly efficient trans-glycosidase activity. ..
  9. Márquez V, Arias D, Chiribao M, Faral Tello P, Robello C, Iglesias A, et al. Redox metabolism in Trypanosoma cruzi. Biochemical characterization of dithiol glutaredoxin dependent cellular pathways. Biochimie. 2014;106:56-67 pubmed publisher
    ..cruzi. The influence of TcrGrx in several parasite physiological processes suggests novel insights about the protein involvement in redox signaling. ..
  10. González Chávez Z, Olin Sandoval V, Rodíguez Zavala J, Moreno Sánchez R, Saavedra E. Metabolic control analysis of the Trypanosoma cruzi peroxide detoxification pathway identifies tryparedoxin as a suitable drug target. Biochim Biophys Acta. 2015;1850:263-73 pubmed publisher
    ..MCA studies provide rational and quantitative criteria to select enzymes for drug-target development. ..
  11. Brandao A, Samudio F, Fernandes O, Calzada J, Sousa O. Genotyping of Panamanian Trypanosoma cruzi stocks using the calmodulin 3'UTR polymorphisms. Parasitol Res. 2008;102:523-6 pubmed publisher
    ..cruzi isolates from humans and vectors from different endemic areas in Panama. The finding of solely T. cruzi I genotype might explain the low morbidity of Chagas' disease in the region when compared to other countries in Latin America. ..
  12. Sepulveda S, Valenzuela L, Ponce I, Sierra S, Bahamondes P, Ramirez S, et al. Expression, functionality, and localization of apurinic/apyrimidinic endonucleases in replicative and non-replicative forms of Trypanosoma cruzi. J Cell Biochem. 2014;115:397-409 pubmed publisher
    ..Our results confirm that the BER pathway is involved in T. cruzi resistance to DNA oxidative damage and points to the participation of DNA AP endonucleases in parasite survival. ..
  13. Martinez A, Peluffo G, Petruk A, Hugo M, Piñeyro D, Demicheli V, et al. Structural and molecular basis of the peroxynitrite-mediated nitration and inactivation of Trypanosoma cruzi iron-superoxide dismutases (Fe-SODs) A and B: disparate susceptibilities due to the repair of Tyr35 radical by Cys83 in Fe-SODB through intra. J Biol Chem. 2014;289:12760-78 pubmed publisher
  14. Pérez Díaz L, Pastro L, Smircich P, Dallagiovanna B, Garat B. Evidence for a negative feedback control mediated by the 3' untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes. Biochem Biophys Res Commun. 2013;436:295-9 pubmed publisher
    ..The mechanism revealed here adds to our limited but growing number of examples of negative mRNA autoregulation in the control of gene expression. ..
  15. Cordeiro A, Cáceres A, Vertommen D, Concepcion J, Michels P, Versées W. The crystal structure of Trypanosoma cruzi glucokinase reveals features determining oligomerization and anomer specificity of hexose-phosphorylating enzymes. J Mol Biol. 2007;372:1215-26 pubmed
    ..The preference for distinct anomers suggests that in T. cruzi hexokinase and glucokinase are not directly competing for the same substrate and are probably both present because they exert distinct physiological functions. ..
  16. Inoue M, Yasuda K, Uemura H, Yasaka N, Inoue H, Sei Y, et al. Phosphorylation-dependent protein interaction with Trypanosoma brucei 14-3-3 proteins that display atypical target recognition. PLoS ONE. 2010;5:e15566 pubmed publisher
    ..This report describes the first discovery of the T. brucei 14-3-3-binding proteins and their binding motifs. The high-affinity phosphopeptide will be a powerful tool to identify novel T. brucei 14-3-3-binding proteins. ..
  17. Cribb P, Perdomo V, Alonso V, Manarin R, Barrios Payán J, Marquina Castillo B, et al. Trypanosoma cruzi High Mobility Group B (TcHMGB) can act as an inflammatory mediator on mammalian cells. PLoS Negl Trop Dis. 2017;11:e0005350 pubmed publisher
  18. Ortiz C, Larrieux N, Medeiros A, Botti H, Comini M, Buschiazzo A. Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:1457-61 pubmed publisher
    ..85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure. ..
  19. Piñeyro M, Parodi Talice A, Portela M, Arias D, Guerrero S, Robello C. Molecular characterization and interactome analysis of Trypanosoma cruzi tryparedoxin 1. J Proteomics. 2011;74:1683-92 pubmed publisher
    ..Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi. ..
  20. Bueren Calabuig J, Pierdominici Sottile G, Roitberg A. Unraveling the differences of the hydrolytic activity of Trypanosoma cruzi trans-sialidase and Trypanosoma rangeli sialidase: a quantum mechanics-molecular mechanics modeling study. J Phys Chem B. 2014;118:5807-16 pubmed publisher
  21. De Vas M, Portal P, Alonso G, Schlesinger M, Flawia M, Torres H, et al. The NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol biosynthesis pathway. Int J Parasitol. 2011;41:99-108 pubmed publisher
    ..In addition, we suggest a different role for TcCPR-A, since its over-expression is lethal, displaying cells with an increased DNA content, aberrant morphology and severe ultrastructural alterations. ..
  22. Osorio Méndez J, Vizcaíno Castillo A, Manning Cela R, Hernandez R, Cevallos A. Expression of profilin in Trypanosoma cruzi and identification of some of its ligands. Biochem Biophys Res Commun. 2016;480:709-714 pubmed publisher
    ..Our work suggests that profilin and the actin system may be regulated by unknown factors and participate in novel biological processes. ..
  23. Martins V, Galizzi M, Salto M, Docampo R, Moreno S. Developmental expression of a Trypanosoma cruzi phosphoinositide-specific phospholipase C in amastigotes and stimulation of host phosphoinositide hydrolysis. Infect Immun. 2010;78:4206-12 pubmed publisher
    ..The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction. ..
  24. Guzmán Luna V, Quezada A, Díaz Salazar A, Cabrera N, Perez Montfort R, Costas M. The effect of specific proline residues on the kinetic stability of the triosephosphate isomerases of two trypanosomes. Proteins. 2017;85:571-579 pubmed publisher
    ..Proteins 2017; 85:571-579. © 2016 Wiley Periodicals, Inc. ..
  25. Garcia Marchan Y, Sojo F, Rodriguez E, Zerpa N, Malave C, Galindo Castro I, et al. Trypanosoma cruzi calmodulin: cloning, expression and characterization. Exp Parasitol. 2009;123:326-33 pubmed publisher
    ..cruzi CaM. ..
  26. Inaoka D, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, et al. Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction. Biochemistry. 2008;47:10881-91 pubmed publisher
  27. Inaoka D, Takashima E, Osanai A, Shimizu H, Nara T, Aoki T, et al. Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:875-8 pubmed
    ..87, b = 71.89, c = 123.27 A. The presence of two molecules in the asymmetric unit (2 x 34 kDa) gives a crystal volume per protein weight (VM) of 2.2 A3 Da(-1) and a solvent content of 44%. ..
  28. Niyogi S, Docampo R. A novel role of Rab11 in trafficking GPI-anchored trans-sialidase to the plasma membrane of Trypanosoma cruzi. Small Gtpases. 2015;6:8-10 pubmed publisher
    ..We also found that traffic of other GPI-anchored proteins is also through the CVC but uses a Rab11-independent pathway. These represent unconventional pathways of GPI-anchored protein traffic to the plasma membrane. ..
  29. Marquez V, Arias D, Piattoni C, Robello C, Iglesias A, Guerrero S. Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi. Antioxid Redox Signal. 2010;12:787-92 pubmed publisher
    ..Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins...
  30. Paes L, Su rez Mantilla B, Zimbres F, Pral E, Diogo de Melo P, Tahara E, et al. Proline dehydrogenase regulates redox state and respiratory metabolism in Trypanosoma cruzi. PLoS ONE. 2013;8:e69419 pubmed publisher
    ..cruzi life cycle...
  31. Lima L, Ortiz P, da Silva F, Alves J, Serrano M, Cortez A, et al. Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species. PLoS ONE. 2012;7:e38385 pubmed publisher
    ..Our findings corroborate cruzipain as valuable target for drugs, vaccine, diagnostic and genotyping approaches. ..
  32. Baptista C, V ncio R, Abdala S, Carranza J, Westenberger S, Silva M, et al. Differential transcription profiles in Trypanosoma cruzi associated with clinical forms of Chagas disease: Maxicircle NADH dehydrogenase subunit 7 gene truncation in asymptomatic patient isolates. Mol Biochem Parasitol. 2006;150:236-48 pubmed publisher
  33. Fernandez P, Haouz A, Pereira C, Aguilar C, Alzari P. The crystal structure of Trypanosoma cruzi arginine kinase. Proteins. 2007;69:209-12 pubmed publisher
  34. Pérez Brandan C, Padilla A, Xu D, Tarleton R, Basombrio M. Knockout of the dhfr-ts gene in Trypanosoma cruzi generates attenuated parasites able to confer protection against a virulent challenge. PLoS Negl Trop Dis. 2011;5:e1418 pubmed publisher
    ..This study indicates that it is possible to generate genetically attenuated T. cruzi parasites able to confer protection against further T. cruzi infections...
  35. Wang H, Kunz S, Chen G, Seebeck T, Wan Y, Robinson H, et al. Biological and structural characterization of Trypanosoma cruzi phosphodiesterase C and Implications for design of parasite selective inhibitors. J Biol Chem. 2012;287:11788-97 pubmed publisher
    ..The structural study also identified a unique parasite pocket that neighbors the active site and may thus be valuable for the design of parasite-specific inhibitors...
  36. da Cunha J, Nakayasu E, de Almeida I, Schenkman S. Post-translational modifications of Trypanosoma cruzi histone H4. Mol Biochem Parasitol. 2006;150:268-77 pubmed publisher
    ..cruzi histone H4 indicate that lysine 4 is acetylated in the majority of histone H4, while other acetylations at the N-terminus portion of histone H4 are less abundant...
  37. Marciano D, Santana M, Nowicki C. Functional characterization of enzymes involved in cysteine biosynthesis and H(2)S production in Trypanosoma cruzi. Mol Biochem Parasitol. 2012;185:114-20 pubmed publisher
  38. Chung J, Rocha A, Tonelli R, Castilho B, Schenkman S. Eukaryotic initiation factor 5A dephosphorylation is required for translational arrest in stationary phase cells. Biochem J. 2013;451:257-67 pubmed publisher
    ..We conclude that eIF5A phosphorylation and dephosphorylation cycles regulate translation according to the growth conditions...
  39. Bermejo D, Jackson S, Gorosito Serrán M, Acosta Rodriguez E, Amezcua Vesely M, Sather B, et al. Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors ROR?t and Ahr that leads to IL-17 production by activated B cells. Nat Immunol. 2013;14:514-22 pubmed publisher
    ..Our combined data suggest that the generation of IL-17(+) B cells may be a previously unappreciated feature of innate immune responses required for pathogen control or IL-17-mediated autoimmunity...
  40. Ochaya S, Respuela P, Simonsson M, Saraswathi A, Branche C, Lee J, et al. Characterization of a Trypanosoma cruzi acetyltransferase: cellular location, activity and structure. Mol Biochem Parasitol. 2007;152:123-31 pubmed publisher
    ..The native protein has a cytosolic cellular location and is present in all three life-cycle stages of the parasite. The recombinant protein was shown to have autoacetylation enzymatic activity...
  41. Portal P, Fern ndez Villamil S, Alonso G, De Vas M, Flawi M, Torres H, et al. Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance. Mol Biochem Parasitol. 2008;160:42-51 pubmed publisher
    ..Overexpression of TcCPR-B in T. cruzi epimastigotes increased its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole. We suggest a participation of TcCPR-B in the detoxification metabolism of the parasite...
  42. Jha B, Archer S, Clayton C. The trypanosome Pumilio domain protein PUF5. PLoS ONE. 2013;8:e77371 pubmed publisher
    ..Knockdown of PUF5 did not have any effect on the ability of trypanosomes to differentiate from the mammalian to the insect form of the parasite...
  43. Cribb P, Perozzi M, Villanova G, Trochine A, Serra E. Characterization of TcHMGB, a high mobility group B family member protein from Trypanosoma cruzi. Int J Parasitol. 2011;41:1149-56 pubmed publisher
    ..Protein levels, however, seem to vary throughout the life cycle, which may be related to previously described changes in heterochromatin distribution and transcription rates...
  44. Rodrigues D, Silva R, Rondinelli E, Urm nyi T. Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock. Exp Parasitol. 2010;126:245-53 pubmed publisher
    ..We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi...
  45. Ecco G, Vernal J, Razzera G, Tavares C, Serpa V, Arias S, et al. Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database. Gene. 2009;448:1-6 pubmed publisher
    ..cruzi kynureninase. Further experimental assays will be performed to fully understand the importance of this enzyme for T. cruzi metabolism...
  46. Miranda M, Bouvier L, Canepa G, Pereira C. Subcellular localization of Trypanosoma cruzi arginine kinase. Parasitology. 2009;136:1201-7 pubmed publisher
    ..The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites...
  47. Bercovich N, Levin M, Clayton C, Vazquez M. Identification of core components of the exon junction complex in trypanosomes. Mol Biochem Parasitol. 2009;166:190-3 pubmed publisher
    ..We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport...
  48. Arias D, Cabeza M, Erben E, Carranza P, Lujan H, T llez I n M, et al. Functional characterization of methionine sulfoxide reductase A from Trypanosoma spp. Free Radic Biol Med. 2011;50:37-46 pubmed publisher
    ..cruzi and Trypanosoma brucei. The results support the occurrence of a metabolic pathway in Trypanosoma spp. involved in the critical function of repairing oxidized macromolecules...
  49. Leroux A, Maugeri D, Opperdoes F, Cazzulo J, Nowicki C. Comparative studies on the biochemical properties of the malic enzymes from Trypanosoma cruzi and Trypanosoma brucei. FEMS Microbiol Lett. 2011;314:25-33 pubmed publisher
  50. Pierdominici Sottile G, Roitberg A. Proton transfer facilitated by ligand binding. An energetic analysis of the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Biochemistry. 2011;50:836-42 pubmed publisher
    ..This study represents an initial step that can shed light on our understanding of the catalytic mechanism of this reaction...
  51. Vilchez Larrea S, Alonso G, Schlesinger M, Torres H, Flawi M, Fern ndez Villamil S. Poly(ADP-ribose) polymerase plays a differential role in DNA damage-response and cell death pathways in Trypanosoma cruzi. Int J Parasitol. 2011;41:405-16 pubmed publisher
    ..We also believe that we provide the first reported evidence that different proteins could be poly(ADP-ribosyl)ated in response to different stimuli, leading to different cell death pathways...
  52. de Moura M, Schamber Reis B, Passos Silva D, Raj o M, Macedo A, Franco G, et al. Cloning and characterization of DNA polymerase eta from Trypanosoma cruzi: roles for translesion bypass of oxidative damage. Environ Mol Mutagen. 2009;50:375-86 pubmed publisher
    ..However the overexpression of the gene only interferes in response to oxidative lesions, possibly due to the presence of these lesions during the S phase...
  53. Raj o M, Passos Silva D, DaRocha W, Franco G, Macedo A, Pena S, et al. DNA polymerase kappa from Trypanosoma cruzi localizes to the mitochondria, bypasses 8-oxoguanine lesions and performs DNA synthesis in a recombination intermediate. Mol Microbiol. 2009;71:185-97 pubmed publisher
    ..cruzi resistance to high doses of gamma irradiation and zeocin and can catalyse DNA synthesis within recombination intermediates...
  54. Bouvier L, Miranda M, Canepa G, Alves M, Pereira C. An expanded adenylate kinase gene family in the protozoan parasite Trypanosoma cruzi. Biochim Biophys Acta. 2006;1760:913-21 pubmed publisher
    ..coli. This work reports an unusually large number of genes of adenylate kinases and suggests a coordinated regulation of phosphotransferase-mediated ATP regenerating pathways in the unicellular parasite Trypanosoma cruzi...
  55. Ekanayake D, Sabatini R. Epigenetic regulation of polymerase II transcription initiation in Trypanosoma cruzi: modulation of nucleosome abundance, histone modification, and polymerase occupancy by O-linked thymine DNA glucosylation. Eukaryot Cell. 2011;10:1465-72 pubmed publisher
  56. Figueiredo da Silva A, de Carvalho Vieira L, Krieger M, Goldenberg S, Zanchin N, Guimar es B. Crystal structure of chagasin, the endogenous cysteine-protease inhibitor from Trypanosoma cruzi. J Struct Biol. 2007;157:416-23 pubmed publisher
    ..The knowledge of the chagasin crystal structure may contribute to the elucidation of the molecular mechanism involved in the inhibition of cruzain and other T. cruzi cysteine proteases...
  57. Gabelli S, McLellan J, Montalvetti A, Oldfield E, Docampo R, Amzel L. Structure and mechanism of the farnesyl diphosphate synthase from Trypanosoma cruzi: implications for drug design. Proteins. 2006;62:80-8 pubmed publisher
  58. Rubin de Celis S, Uemura H, Yoshida N, Schenkman S. Expression of trypomastigote trans-sialidase in metacyclic forms of Trypanosoma cruzi increases parasite escape from its parasitophorous vacuole. Cell Microbiol. 2006;8:1888-98 pubmed publisher
    ..These results indicate that the increased expression of TS in cell-derived trypomastigotes is responsible for the earlier exit from the PV to the cytoplasm and their subsequent differentiation into amastigotes...
  59. Woronowicz A, Amith S, Davis V, Jayanth P, De Vusser K, Laroy W, et al. Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells. Glycobiology. 2007;17:725-34 pubmed publisher
    ..Together, these observations suggest that trypanosome TS mimics neurotrophic factors in cell survival responses against oxidative stress, hypoxia-induced neurite retraction and serum/glucose deprivation...
  60. Ljunggren A, Redzynia I, Alvarez Fernandez M, Abrahamson M, Mort J, Krupa J, et al. Crystal structure of the parasite protease inhibitor chagasin in complex with a host target cysteine protease. J Mol Biol. 2007;371:137-53 pubmed publisher
    ..This information will aid in the development of synthetic inhibitors for use as potential drugs for the treatment of Chagas disease...
  61. Buchanan K, Ames J, Asfaw S, Wingard J, Olson C, Campana P, et al. A flagellum-specific calcium sensor. J Biol Chem. 2005;280:40104-11 pubmed publisher
    ..These findings may have broader implications for the calcium acyl switch mechanism of protein regulation...
  62. Piacenza L, Peluffo G, Alvarez M, Kelly J, Wilkinson S, Radi R. Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite. Biochem J. 2008;410:359-68 pubmed publisher
    ..Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance...
  63. Araya J, Cornejo A, Orrego P, Cordero E, Cort z M, Olivares H, et al. Calcineurin B of the human protozoan parasite Trypanosoma cruzi is involved in cell invasion. Microbes Infect. 2008;10:892-900 pubmed publisher
    ..Given that TcCaNB-CL may play a key role in cell invasion and differs considerably in its primary structure from the human CaNB, it might be considered as a potential chemotherapeutic target...
  64. Uehara L, Moreira O, Oliveira A, Azambuja P, Lima A, Britto C, et al. Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. PLoS Negl Trop Dis. 2012;6:e1958 pubmed publisher
    ..These data highlight, for the first time, that cruzipain contributes to the interaction of T. cruzi with the insect host. ..
  65. Ram rez G, Valck C, Aguilar L, Kemmerling U, L pez Mu oz R, Cabrera G, et al. Roles of Trypanosoma cruzi calreticulin in parasite-host interactions and in tumor growth. Mol Immunol. 2012;52:133-40 pubmed publisher
    ..A different domain, amino terminal, binds to endothelial cells, thus inhibiting their angiogenic capacity. Since tumor growth depends, to a large extent on angiogenesis, their growth is also inhibited...
  66. Ratier L, Urrutia M, Paris G, Zarebski L, Frasch A, Goldbaum F. Relevance of the diversity among members of the Trypanosoma cruzi trans-sialidase family analyzed with camelids single-domain antibodies. PLoS ONE. 2008;3:e3524 pubmed publisher
    ..cruzi to evade the host immune system...
  67. Cuellar A, Rojas F, Bolaños N, Diez H, Del Carmen Thomas M, Rosas F, et al. Natural CD4(+) T-cell responses against Trypanosoma cruzi KMP-11 protein in chronic chagasic patients. Immunol Cell Biol. 2009;87:149-53 pubmed publisher
    ..This is the first description of T. cruzi KMP-11 protein recognition by CD4(+) T cells in chronic chagasic patients. ..
  68. Miranda M, Canepa G, Bouvier L, Pereira C. Trypanosoma cruzi nucleoside diphosphate kinase 1 ( TcNDPK1) has a broad nuclease activity. Parasitology. 2008;135:1661-6 pubmed publisher
    ..Furthermore, phenogram analysis showed that TcNDPK1 is close to Drosophila melanogaster and human NDPKs. The unspecific nuclease activity could suggest a participation in cellular processes such as programmed cell death...
  69. Gallo G, Ramos T, Tavares F, Rocha A, Machi E, Schenkman S, et al. Biochemical characterization of a protein tyrosine phosphatase from Trypanosoma cruzi involved in metacyclogenesis and cell invasion. Biochem Biophys Res Commun. 2011;408:427-31 pubmed publisher
    ..In conclusion our results indicate that TcPTP1 is of importance for cellular differentiation and invasivity of this parasite and thus is a valid target for the rational drug design of potential antibiotics directed against T. cruzi...
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    ..1°C and pH 8.6. Above 37°C, the enzyme activity starts to fall, which may be related to previous reports that the quaternary structure begins a process of disassembly...
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    ..cruzi. While K4 acetylation occurs preferentially in proliferating situations and accumulates in packed chromatin, K10 and K14 acetylations have a particular distribution probably at the boundaries between packed and unpacked chromatin...
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    ..The essentiality of the single HisRS gene in T. brucei is shown by a severe depression of parasite growth rate that results from even partial suppression of expression by RNA interference...
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