Streptomyces coelicolor A3(2)



Top Publications

  1. Morais Cabral J, Zhou Y, MacKinnon R. Energetic optimization of ion conduction rate by the K+ selectivity filter. Nature. 2001;414:37-42 pubmed
    ..The energetic balance between these configurations is a clear example of evolutionary optimization of protein function...
  2. Perozo E, Cortes D, Cuello L. Structural rearrangements underlying K+-channel activation gating. Science. 1999;285:73-8 pubmed
    ..Although the extracellular residues flanking the selectivity filter remained immobile during gating, small movements were detected at the C-terminal end of the pore helix, with possible implications to the gating mechanism...
  3. Gust B, Challis G, Fowler K, Kieser T, Chater K. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci U S A. 2003;100:1541-6 pubmed
  4. Grantcharova N, Lustig U, Flärdh K. Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2). J Bacteriol. 2005;187:3227-37 pubmed
    ..They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription. ..
  5. Mistry B, Del Sol R, Wright C, Findlay K, Dyson P. FtsW is a dispensable cell division protein required for Z-ring stabilization during sporulation septation in Streptomyces coelicolor. J Bacteriol. 2008;190:5555-66 pubmed publisher
  6. Holmes N, Walshaw J, Leggett R, Thibessard A, Dalton K, Gillespie M, et al. Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces. Proc Natl Acad Sci U S A. 2013;110:E397-406 pubmed publisher
  7. Youn H, Kim E, Roe J, Hah Y, Kang S. A novel nickel-containing superoxide dismutase from Streptomyces spp. Biochem J. 1996;318 ( Pt 3):889-96 pubmed
    ..The apoenzymes, lacking in nickel, had no ability to mediate the conversion of superoxide anion radical to hydrogen peroxide, strongly indicating that NiIII plays a main role in these enzymes. ..
  8. Onaka H, Nakagawa T, Horinouchi S. Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of secondary metabolism and morphogenesis. Mol Microbiol. 1998;28:743-53 pubmed
    ..It is thus concluded that both cprA and cprB play regulatory roles in both secondary metabolism and morphogenesis in S. coelicolor A3(2), just as the arpA/A-factor system in Streptomyces griseus. ..
  9. Zhou Y, Morais Cabral J, Kaufman A, MacKinnon R. Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution. Nature. 2001;414:43-8 pubmed
    ..This structural change is crucial to the operation of the selectivity filter in the cellular context, where the K+ ion concentration near the selectivity filter varies in response to channel gating. ..

More Information

Publications159 found, 100 shown here

  1. Zhou Y, MacKinnon R. The occupancy of ions in the K+ selectivity filter: charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates. J Mol Biol. 2003;333:965-75 pubmed
    ..We conclude that electrostatic balance and coupling of ion binding to a protein conformational change underlie high conduction rates in the setting of high selectivity...
  2. Guthrie E, Flaxman C, White J, Hodgson D, Bibb M, Chater K. A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3(2) with an amino-terminal domain that lacks a phosphorylation pocket. Microbiology. 1998;144 ( Pt 3):727-38 pubmed
  3. Kim F, Kim H, Hah Y, Roe J. Differential expression of superoxide dismutases containing Ni and Fe/Zn in Streptomyces coelicolor. Eur J Biochem. 1996;241:178-85 pubmed
    ..The changes in SOD activities were positively correlated with the amount of each enzyme as determined by immunoblotting, suggesting that metals do not modulate the activity per se but the amount of each protein. ..
  4. Ryding N, Kelemen G, Whatling C, Flärdh K, Buttner M, Chater K. A developmentally regulated gene encoding a repressor-like protein is essential for sporulation in Streptomyces coelicolor A3(2). Mol Microbiol. 1998;29:343-57 pubmed
    ..PwhiH was directly dependent on the sigma factor encoded by another sporulation gene, whiG, as shown by in vivo and in vitro transcription analysis. ..
  5. Paget M, Leibovitz E, Buttner M. A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2). Mol Microbiol. 1999;33:97-107 pubmed
    ..We propose a model in which the CseB/CseC two-component system modulates activity of the sigE promoter in response to signals from the cell envelope. ..
  6. Viollier P, Nguyen K, Minas W, Folcher M, Dale G, Thompson C. Roles of aconitase in growth, metabolism, and morphological differentiation of Streptomyces coelicolor. J Bacteriol. 2001;183:3193-203 pubmed
    ..Immunoblots revealed that AcoA was present primarily during substrate mycelial growth on solid medium. Transcription of acoA was limited to the early growth phase in liquid cultures from a start site mapped in vitro and in vivo. ..
  7. Rigali S, Nothaft H, Noens E, Schlicht M, Colson S, Müller M, et al. The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR-family regulator DasR and links N-acetylglucosamine metabolism to the control of development. Mol Microbiol. 2006;61:1237-51 pubmed
    ..Our work, and the model it suggests, provide new leads towards understanding how microorganisms time developmental commitment. ..
  8. Valiyaveetil F, Leonetti M, Muir T, MacKinnon R. Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation. Science. 2006;314:1004-7 pubmed
  9. Hsiao N, Söding J, Linke D, Lange C, Hertweck C, Wohlleben W, et al. ScbA from Streptomyces coelicolor A3(2) has homology to fatty acid synthases and is able to synthesize gamma-butyrolactones. Microbiology. 2007;153:1394-404 pubmed
  10. Tiffert Y, Supra P, Wurm R, Wohlleben W, Wagner R, Reuther J. The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes. Mol Microbiol. 2008;67:861-80 pubmed publisher
    ..coelicolor GlnR is able to interact with these glnA upstream regions. We therefore suggest that GlnR-mediated regulation is not restricted to Streptomyces but constitutes a regulon conserved in many actinomycetes. ..
  11. Santos Beneit F, Rodr guez Garc a A, Franco Dom nguez E, Mart n J. Phosphate-dependent regulation of the low- and high-affinity transport systems in the model actinomycete Streptomyces coelicolor. Microbiology. 2008;154:2356-70 pubmed publisher
    ..A model for PhoP regulation of this promoter is proposed based on the four promoter DNA-PhoP complexes detected by electrophoretic mobility shift assays and footprinting studies...
  12. Xu X, Wang Z, Fan K, Wang S, Jia C, Han H, et al. Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. FEMS Microbiol Lett. 2008;287:15-21 pubmed publisher
  13. Willemse J, van Wezel G. Imaging of Streptomyces coelicolor A3(2) with reduced autofluorescence reveals a novel stage of FtsZ localization. PLoS ONE. 2009;4:e4242 pubmed publisher
    ..The enhanced imaging properties are an important step forward for the confocal and live imaging of less abundant proteins and for the use of lower intensity fluorophores in streptomycetes. ..
  14. Arabolaza A, D Angelo M, Comba S, Gramajo H. FasR, a novel class of transcriptional regulator, governs the activation of fatty acid biosynthesis genes in Streptomyces coelicolor. Mol Microbiol. 2010;78:47-63 pubmed publisher
    ..These data provide the first example of positive regulation of genes encoding core proteins of saturated fatty acid synthase complex. ..
  15. den Hengst C, Tran N, Bibb M, Chandra G, Leskiw B, Buttner M. Genes essential for morphological development and antibiotic production in Streptomyces coelicolor are targets of BldD during vegetative growth. Mol Microbiol. 2010;78:361-79 pubmed
    ..High-scoring copies of the BldD binding site were found at relevant positions in the genomes of other bacteria containing a BldD homologue, suggesting the role of BldD is conserved in sporulating actinomycetes. ..
  16. Ditkowski B, Troć P, Ginda K, Donczew M, Chater K, Zakrzewska Czerwinska J, et al. The actinobacterial signature protein ParJ (SCO1662) regulates ParA polymerization and affects chromosome segregation and cell division during Streptomyces sporulation. Mol Microbiol. 2010;78:1403-15 pubmed publisher
    ..We hypothesize that polar growth, which is characteristic not only of streptomycetes, but even of simple Actinobacteria, may be interlinked with ParA polymer assembly and its specific regulation by ParJ. ..
  17. Wang J, Wang W, Wang L, Zhang G, Fan K, Tan H, et al. A novel role of 'pseudo'?-butyrolactone receptors in controlling ?-butyrolactone biosynthesis in Streptomyces. Mol Microbiol. 2011;82:236-50 pubmed publisher
  18. Holley T, Stevenson C, Bibb M, Lawson D. High resolution crystal structure of Sco5413, a widespread actinomycete MarR family transcriptional regulator of unknown function. Proteins. 2013;81:176-82 pubmed publisher
  19. Doyle D, Morais Cabral J, Pfuetzner R, Kuo A, Gulbis J, Cohen S, et al. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science. 1998;280:69-77 pubmed
    ..The architecture of the pore establishes the physical principles underlying selective K+ conduction...
  20. Soliveri J, Gomez J, Bishai W, Chater K. Multiple paralogous genes related to the Streptomyces coelicolor developmental regulatory gene whiB are present in Streptomyces and other actinomycetes. Microbiology. 2000;146 ( Pt 2):333-43 pubmed
  21. Martínez Costa O, Zalacain M, Holmes D, Malpartida F. The promoter of a cold-shock-like gene has pleiotropic effects on Streptomyces antibiotic biosynthesis. FEMS Microbiol Lett. 2003;220:215-21 pubmed
    ..Interestingly, the csp1-groEL2 region pleiotropically regulates the production of antibiotics from Streptomyces coelicolor and Streptomyces nodosus. ..
  22. Natsume R, Ohnishi Y, Senda T, Horinouchi S. Crystal structure of a gamma-butyrolactone autoregulator receptor protein in Streptomyces coelicolor A3(2). J Mol Biol. 2004;336:409-19 pubmed
  23. Kodani S, Hudson M, Durrant M, Buttner M, Nodwell J, Willey J. The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolor. Proc Natl Acad Sci U S A. 2004;101:11448-53 pubmed
    ..We conclude that SapB is derived from RamS through proteolytic cleavage and the introduction of four dehydroalanine residues and two lanthionine bridges. We provide an example of a morphogenetic role for an antibiotic-like molecule. ..
  24. Walter S, Schrempf H. Characteristics of the surface-located carbohydrate-binding protein CbpC from Streptomyces coelicolor A32. Arch Microbiol. 2008;190:119-27 pubmed publisher
    ..As demonstrated by analysing further truncated CbpC-forms a glycine-aspartate/serine rich region, which separates the carbohydrate-binding module from the sorting signal, plays an important role in protein stability...
  25. Hempel A, Wang S, Letek M, Gil J, Flärdh K. Assemblies of DivIVA mark sites for hyphal branching and can establish new zones of cell wall growth in Streptomyces coelicolor. J Bacteriol. 2008;190:7579-83 pubmed publisher
    ..Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and cell shape in Streptomyces. ..
  26. Santos Beneit F, Rodr guez Garc a A, Sola Landa A, Mart n J. Cross-talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription. Mol Microbiol. 2009;72:53-68 pubmed publisher
    ..The reciprocal regulation of the phoRP promoter by AfsR and of afsS by PhoP suggests a fine interplay of these regulators on the control of secondary metabolism...
  27. González Cerón G, Miranda Olivares O, Servín Gonzalez L. Characterization of the methyl-specific restriction system of Streptomyces coelicolor A3(2) and of the role played by laterally acquired nucleases. FEMS Microbiol Lett. 2009;301:35-43 pubmed publisher
    ..Cloning of these genes in the close relative Streptomyces lividans increased the restriction of methylated DNA by this species, confirming their role as part of the methyl-specific restriction system of S. coelicolor...
  28. Aínsa J, Bird N, Ryding N, Findlay K, Chater K. The complex whiJ locus mediates environmentally sensitive repression of development of Streptomyces coelicolor A3(2). Antonie Van Leeuwenhoek. 2010;98:225-36 pubmed publisher
    ..It is suggested that this activity of SCO4542 protein is prevented by an unknown signal. ..
  29. Liu G, Ou H, Wang T, Li L, Tan H, Zhou X, et al. Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA. PLoS Genet. 2010;6:e1001253 pubmed publisher
    ..This is the first report of in vitro endonuclease activity of a McrA homologue and also the first demonstration of an enzyme that specifically cleaves S-modified DNA. ..
  30. De Crecy Lagard V, Servant Moisson P, Viala J, Grandvalet C, Mazodier P. Alteration of the synthesis of the Clp ATP-dependent protease affects morphological and physiological differentiation in Streptomyces. Mol Microbiol. 1999;32:505-17 pubmed
    ..Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans, S. albus and S. coelicolor. Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans. ..
  31. Dedrick R, Wildschutte H, McCormick J. Genetic interactions of smc, ftsK, and parB genes in Streptomyces coelicolor and their developmental genome segregation phenotypes. J Bacteriol. 2009;191:320-32 pubmed publisher
  32. Vogtli M, Chang P, Cohen S. afsR2: a previously undetected gene encoding a 63-amino-acid protein that stimulates antibiotic production in Streptomyces lividans. Mol Microbiol. 1994;14:643-53 pubmed
    ..Analysis of the cloned afsR2 gene indicates that its activity is the result of the 63-amino-acid protein it specifies. ..
  33. Fernandez Moreno M, Caballero J, Hopwood D, Malpartida F. The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces. Cell. 1991;66:769-80 pubmed
    ..We conclude that initiation of actinorhodin synthesis via the actII-ORF4 product, and the final step in production, antibiotic export, are twin targets via which bldA exerts developmental control of actinorhodin production. ..
  34. McCormick J, Su E, Driks A, Losick R. Growth and viability of Streptomyces coelicolor mutant for the cell division gene ftsZ. Mol Microbiol. 1994;14:243-54 pubmed
  35. Feeney M, Chandra G, Findlay K, Paget M, Buttner M. Translational Control of the SigR-Directed Oxidative Stress Response in Streptomyces via IF3-Mediated Repression of a Noncanonical GTC Start Codon. MBio. 2017;8: pubmed publisher
    ..It also emphasizes the limitations of predicting start codons using bioinformatic approaches, which rely heavily on the assumption that ATG, GTG, and TTG are the only permissible start codons. ..
  36. Di Salvo M, Pinatel E, Tala A, Fondi M, Peano C, Alifano P. G4PromFinder: an algorithm for predicting transcription promoters in GC-rich bacterial genomes based on AT-rich elements and G-quadruplex motifs. BMC Bioinformatics. 2018;19:36 pubmed publisher
    ..coelicolor A3(2). Moreover consensus-based tools and, in general, tools that are based on specific features of bacterial σ factors seem to be less performing for promoter prediction in these types of bacterial genomes. ..
  37. Schwartz D, Recktenwald J, Pelzer S, Wohlleben W. Isolation and characterization of the PEP-phosphomutase and the phosphonopyruvate decarboxylase genes from the phosphinothricin tripeptide producer Streptomyces viridochromogenes Tü494. FEMS Microbiol Lett. 1998;163:149-57 pubmed
    ..The ppm gene encodes a protein which is similar to PEP-phosphomutases and the deduced Ppd product shows similarity to the phosphonopyruvate decarboxylase from Streptomyces wedmorensis. ..
  38. Cho Y, Lee E, Roe J. A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor. Mol Microbiol. 2000;35:150-60 pubmed
    ..These results suggest that regulated synthesis of CatB protein is necessary to ensure proper differentiation as well as to protect S. coelicolor cells against osmotic stresses...
  39. Zhou M, Morais Cabral J, Mann S, MacKinnon R. Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors. Nature. 2001;411:657-61 pubmed publisher
    ..This mechanism accounts for the functional properties of K+ channel inactivation and indicates that the cavity may be the site of action for certain drugs that alter cation channel function...
  40. Huang J, Lih C, Pan K, Cohen S. Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev. 2001;15:3183-92 pubmed publisher
  41. Fujimoto Z, Kuno A, Kaneko S, Kobayashi H, Kusakabe I, Mizuno H. Crystal structures of the sugar complexes of Streptomyces olivaceoviridis E-86 xylanase: sugar binding structure of the family 13 carbohydrate binding module. J Mol Biol. 2002;316:65-78 pubmed publisher
    ..In such enzyme, XBD binds xylan, and the catalytic domain may assume a flexible position with respect to the XBD/xylan complex, inasmuch as the linker region is unstructured...
  42. van Wezel G, Mahr K, K nig M, Traag B, Pimentel Schmitt E, Willimek A, et al. GlcP constitutes the major glucose uptake system of Streptomyces coelicolor A3(2). Mol Microbiol. 2005;55:624-36 pubmed publisher
    ..It is anticipated that the activity of GlcP is linked to other glucose-mediated phenomena such as carbon catabolite repression, morphogenesis and antibiotic production...
  43. Kim D, Chater K, Lee K, Hesketh A. Changes in the extracellular proteome caused by the absence of the bldA gene product, a developmentally significant tRNA, reveal a new target for the pleiotropic regulator AdpA in Streptomyces coelicolor. J Bacteriol. 2005;187:2957-66 pubmed publisher
    ..Mutation of the SCO0762 gene abolished detectable trypsin-protease inhibitory activity but did not result in any obvious morphological defects...
  44. Cordero Morales J, Cuello L, Zhao Y, Jogini V, Cortes D, Roux B, et al. Molecular determinants of gating at the potassium-channel selectivity filter. Nat Struct Mol Biol. 2006;13:311-8 pubmed publisher
    ..These data establish a mechanistic basis for the role of the selectivity filter during channel activation and inactivation...
  45. Brnáková Z, Godany A, Timko J. An exodeoxyribonuclease from Streptomyces coelicolor: expression, purification and biochemical characterization. Biochim Biophys Acta. 2007;1770:630-7 pubmed
    ..5 for either ssDNA or dsDNA substrates. It required a divalent cation (Mg(2+), Co(2+), Ca(2+)) and its activity was strongly inhibited in the presence of Zn(2+), Hg(2+), chelating agents or iodoacetate. ..
  46. Sevcikova B, Rezuchova B, Homerova D, Kormanec J. The anti-anti-sigma factor BldG is involved in activation of the stress response sigma factor ?(H) in Streptomyces coelicolor A3(2). J Bacteriol. 2010;192:5674-81 pubmed publisher
    ..coelicolor bldG mutant, indicating a role of BldG in ?(H) activation by a partner-switching-like mechanism...
  47. Bibby J, Keegan R, Mayans O, Winn M, Rigden D. Application of the AMPLE cluster-and-truncate approach to NMR structures for molecular replacement. Acta Crystallogr D Biol Crystallogr. 2013;69:2194-201 pubmed publisher
    ..Potential future routes to improved performance are considered and practical, general guidelines on using AMPLE are provided. ..
  48. Takano H, Hagiwara K, Ueda K. Fundamental role of cobalamin biosynthesis in the developmental growth of Streptomyces coelicolor A3 (2). Appl Microbiol Biotechnol. 2015;99:2329-37 pubmed publisher
    ..Effective Cbl production is fundamental to the diverse functions underlying the complex developmental life cycle of S. coelicolor A3 (2). ..
  49. Nakajima S, Satoh Y, Yanashima K, Matsui T, Dairi T. Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses. J Biosci Bioeng. 2015;120:294-8 pubmed publisher
    ..In contrast, the amount of ERG was almost the same between the MSH-disruptant and the parental strain. Taken together, our results suggest that ERG is more important than MSH in S. coelicolor A3(2). ..
  50. Iwagami S, Yang K, Davies J. Characterization of the protocatechuic acid catabolic gene cluster from Streptomyces sp. strain 2065. Appl Environ Microbiol. 2000;66:1499-508 pubmed
    ..The pcaIJ genes encoded proteins with a striking similarity to succinyl-coenzyme A (CoA):3-oxoacid CoA transferases of eukaryotes and contained an indel which is strikingly similar between high-G+C gram-positive bacteria and eukaryotes...
  51. Feng W, Mao X, Liu Z, Li Y. The ECF sigma factor SigT regulates actinorhodin production in response to nitrogen stress in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2011;92:1009-21 pubmed publisher
    ..Phenotypic and transcriptional results suggested RstA may modulate the activity of SigT positively...
  52. Lalić J, Posavec Marjanović M, Palazzo L, Perina D, Sabljić I, Zaja R, et al. Disruption of Macrodomain Protein SCO6735 Increases Antibiotic Production in Streptomyces coelicolor. J Biol Chem. 2016;291:23175-23187 pubmed
    ..coelicolor increases antibiotic production. Our results provide the first insights into the molecular basis of its action and impact on Streptomyces metabolism. ..
  53. Bao K, Cohen S. Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces. Genes Dev. 2001;15:1518-27 pubmed publisher
    ..Surprisingly, Tpg proteins were observed to contain a reverse transcriptase-like domain rather than sequences in common with proteins that attach covalently to the termini of linear DNA replicons...
  54. Walker G, Dunbar B, Hunter I, Nimmo H, Coggins J. Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces coelicolor A3(2), Streptomyces rimosus and Neurospora crassa. Microbiology. 1996;142 ( Pt 8):1973-82 pubmed publisher
    ..These studies indicate that the class containing the plant DAHP synthases also contains enzymes from a microbial eukaryote and from several bacteria...
  55. Betzler M, Tlolka I, Schrempf H. Amplification of a Streptomyces lividans 4.3 kb DNA element causes overproduction of a novel hypha- and vesicle-associated protein. Microbiology. 1997;143 ( Pt 4):1243-52 pubmed
    ..Examination by microscopy revealed that the strain carrying the ADS forms bulges within the substrate hyphae and apical vesicles. These bulges have high levels of associated 23 kDa protein and contain storage-like material. ..
  56. Fern ndez Abalos J, Reviejo V, D az M, Rodr guez S, Leal F, Santamar a R. Posttranslational processing of the xylanase Xys1L from Streptomyces halstedii JM8 is carried out by secreted serine proteases. Microbiology. 2003;149:1623-32 pubmed publisher
    ..halstedii JM8, but do not have any clear effect on other secreted proteins such as amylase (Amy) from Streptomyces griseus and xylanase Xyl30 from Streptomyces avermitilis...
  57. Schwartz D, Berger S, Heinzelmann E, Muschko K, Welzel K, Wohlleben W. Biosynthetic gene cluster of the herbicide phosphinothricin tripeptide from Streptomyces viridochromogenes Tü494. Appl Environ Microbiol. 2004;70:7093-102 pubmed publisher
    ..A previously undescribed regulatory gene involved in morphological differentiation in streptomycetes was identified outside of the left boundary of the PTT biosynthetic gene cluster...
  58. Lenaeus M, Vamvouka M, Focia P, Gross A. Structural basis of TEA blockade in a model potassium channel. Nat Struct Mol Biol. 2005;12:454-9 pubmed publisher
    ..We propose that TEA blocks potassium channels by acting as a potassium analog at the dehydration transition step during permeation...
  59. Deol S, Domene C, Bond P, Sansom M. Anionic phospholipid interactions with the potassium channel KcsA: simulation studies. Biophys J. 2006;90:822-30 pubmed publisher
    ..Overall, this study suggests that simulations can help identify and characterize sites for specific lipid interactions on a membrane protein surface...
  60. Willemse J, Mommaas A, van Wezel G. Constitutive expression of ftsZ overrides the whi developmental genes to initiate sporulation of Streptomyces coelicolor. Antonie Van Leeuwenhoek. 2012;101:619-32 pubmed publisher
    ..Our data shed new light on the longstanding question as to how whi genes control sporulation, which has intrigued scientists for four decades...
  61. Singh R, Mo S, Florova G, Reynolds K. Streptomyces coelicolor RedP and FabH enzymes, initiating undecylprodiginine and fatty acid biosynthesis, exhibit distinct acyl-CoA and malonyl-acyl carrier protein substrate specificities. FEMS Microbiol Lett. 2012;328:32-8 pubmed publisher
  62. Huang H, Grove A. The transcriptional regulator TamR from Streptomyces coelicolor controls a key step in central metabolism during oxidative stress. Mol Microbiol. 2013;87:1151-66 pubmed publisher
  63. Enkhbaatar B, Lee C, Hong Y, Hong S. Molecular Characterization of Xylobiose- and Xylopentaose-Producing ?-1,4-Endoxylanase SCO5931 from Streptomyces coelicolor A3(2). Appl Biochem Biotechnol. 2016;180:349-60 pubmed publisher
    ..Product masses corresponded to sodium adducts of xylobiose (m/z 305.24) and xylopentaose (m/z 701.59), indicating that SCO5931 specifically cleaves the ?-1,4 linkage of xylan to yield xylobiose and xylopentaose. ..
  64. Zerbe Burkhardt K, Ratnatilleke A, Philippon N, Birch A, Leiser A, Vrijbloed J, et al. Cloning, sequencing, expression, and insertional inactivation of the gene for the large subunit of the coenzyme B12-dependent isobutyryl-CoA mutase from Streptomyces cinnamonensis. J Biol Chem. 1998;273:6508-17 pubmed
    ..After purification, this small subunit showed no ICM activity but gave active enzyme when recombined with coenzyme B12 and IcmA or His6-IcmA...
  65. Bralley P, Jones G. Transcriptional analysis and regulation of the sigma-E gene of Streptomyces antibioticus. Biochim Biophys Acta. 2001;1517:410-5 pubmed
    ..Deletion analysis in promoter probes showed that maximal activity of the S. antibioticus promoter depends upon the presence of the sequence surrounding the A-rich box, as well as the region further upstream carrying other direct repeats...
  66. Wendt Pienkowski E, Huang Y, Zhang J, Li B, Jiang H, Kwon H, et al. Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. J Am Chem Soc. 2005;127:16442-52 pubmed publisher
    ..griseus, and heterologous expression of the fdm cluster in Streptomyces albus set the stage to investigate FDM A biosynthesis and engineer the FDM biosynthetic machinery for the production of novel FDM A analogues...
  67. Wang G, Tanaka Y, Ochi K. The G243D mutation (afsB mutation) in the principal sigma factor sigmaHrdB alters intracellular ppGpp level and antibiotic production in Streptomyces coelicolor A3(2). Microbiology. 2010;156:2384-92 pubmed publisher
  68. Mavituna F, Luti K, Gu L. In Search of the E. coli Compounds that Change the Antibiotic Production Pattern of Streptomyces coelicolor During Inter-species Interaction. Enzyme Microb Technol. 2016;90:45-52 pubmed publisher
    ..coli cells and their supernatant indicating that this class of compounds secreted by E. coli indeed could act as actives during interspecies interaction and increase the production of undecylprodigiosin. ..
  69. Ishizuka M, Imai Y, Mukai K, Shimono K, Hamauzu R, Ochi K, et al. A possible mechanism for lincomycin induction of secondary metabolism in Streptomyces coelicolor A3(2). Antonie Van Leeuwenhoek. 2018;111:705-716 pubmed publisher
    ..A possible mechanism for lincomycin induction of secondary metabolism in S. coelicolor A3(2) is discussed on the basis of these results. ..
  70. Shima J, Hesketh A, Okamoto S, Kawamoto S, Ochi K. Induction of actinorhodin production by rpsL (encoding ribosomal protein S12) mutations that confer streptomycin resistance in Streptomyces lividans and Streptomyces coelicolor A3(2). J Bacteriol. 1996;178:7276-84 pubmed
    ..griseus) was not eliminated. These results indicate that the onset and extent of secondary metabolism in Streptomyces spp. is significantly controlled by the translational machinery...
  71. Huddleston A, Cresswell N, Neves M, Beringer J, Baumberg S, Thomas D, et al. Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. Appl Environ Microbiol. 1997;63:1288-97 pubmed
    ..It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production...
  72. Martin M, Schneider D, Bruton C, Chater K, Hardisson C. A glgC gene essential only for the first of two spatially distinct phases of glycogen synthesis in Streptomyces coelicolor A3(2). J Bacteriol. 1997;179:7784-9 pubmed
    ..A second, phase II-specific, glgC gene should also exist in S. coelicolor, though it was not detected by hybridization analysis...
  73. Ogawara H, Aoyagi N, Watanabe M, Urabe H. Sequences and evolutionary analyses of eukaryotic-type protein kinases from Streptomyces coelicolor A3(2). Microbiology. 1999;145 ( Pt 12):3343-52 pubmed
    ..This conclusion is supported by the observation that the prokaryotes retaining several of these kinases undergo complicated morphological and/or biochemical differentiation. ..
  74. Kendrew S, Federici L, Savino C, Miele A, Marsh E, Vallone B. Crystallization and preliminary X-ray diffraction studies of a monooxygenase from Streptomyces coelicolor A3(2) involved in the biosynthesis of the polyketide actinorhodin. Acta Crystallogr D Biol Crystallogr. 2000;56:481-3 pubmed
    ..Structure determination should provide further insight into the enzyme mechanism and aid in the design of biosynthetic pathways to produce new polyketide natural products with novel functionality. ..
  75. Kormanec J, Sevc kov B, Halgasov N, Knirschov R, Rezuchov B. Identification and transcriptional characterization of the gene encoding the stress-response sigma factor sigma(H) in streptomyces coelicolor A3(2). FEMS Microbiol Lett. 2000;189:31-8 pubmed
    ..The other three promoters (P2, P3, and P4) were located upstream of ushX, and were differentially induced after various stress conditions. The magnitude of the induction was greatest after osmotic stress and heat shock...
  76. Wang L, Li S, Li Y. Identification and characterization of a new exopolysaccharide biosynthesis gene cluster from Streptomyces. FEMS Microbiol Lett. 2003;220:21-7 pubmed
    ..Involvement of the ste gene cluster in exopolysaccharide biosynthesis was confirmed by disrupting the priming glycosyltransferase gene in Streptomyces sp. 139 to generate non-exopolysaccharide-producing mutants. ..
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    ..Disruption of chromosomal pkaD resulted in a significant loss of actinorhodin production. This result implies the involvement of pkaD in the regulation of secondary metabolism. ..
  79. Rajesh T, Song E, Kim J, Lee B, Kim E, Park S, et al. Inactivation of phosphomannose isomerase gene abolishes sporulation and antibiotic production in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2012;93:1685-93 pubmed publisher
    ..The present study shows that enzymes involved in carbohydrate metabolism could control cellular differentiation as well as the production of secondary metabolites. ..
  80. Kim J, Lee H, Kim P, Lee H, Kim E. Negative role of wblA in response to oxidative stress in Streptomyces coelicolor. J Microbiol Biotechnol. 2012;22:736-41 pubmed
    ..coelicolor, similar to that of C. glutamicum WhcA, through the transcriptional regulation of oxidative stress-related genes...
  81. Rajesh T, Jeon J, Kim Y, Kim H, Yi D, Park S, et al. Functional analysis of the gene SCO1782 encoding Streptomyces hemolysin (S-hemolysin) in Streptomyces coelicolor M145. Toxicon. 2013;71:159-65 pubmed publisher
    ..Recombinant hemolysin exhibited activity against sheep blood erythrocytes and cytolytic activity against human fibroblast cells. Deletion of SCO1782 resulted in complete loss of hemolysin activity in S. coelicolor. ..
  82. Sexton D, St Onge R, Haiser H, Yousef M, Brady L, Gao C, et al. Resuscitation-promoting factors are cell wall-lytic enzymes with important roles in the germination and growth of Streptomyces coelicolor. J Bacteriol. 2015;197:848-60 pubmed publisher
  83. Schneider D, Bruton C, Chater K. Duplicated gene clusters suggest an interplay of glycogen and trehalose metabolism during sequential stages of aerial mycelium development in Streptomyces coelicolor A3(2). Mol Gen Genet. 2000;263:543-53 pubmed
    ..g. glucose-1-phosphate generated by glycogen phosphorylase) and metabolically inert but physiologically significant (trehalose)...
  84. Kim I, Kim M, Lee C, Yim H, Cha S, Kang S. Crystallization and preliminary X-ray crystallographic analysis of the DNA-binding domain of BldD from Streptomyces coelicolor A3(2). Acta Crystallogr D Biol Crystallogr. 2004;60:1115-7 pubmed
    ..2, b = 31.8, c = 33.6 angstroms, beta = 105.1 degrees. Analysis of the packing density shows that the asymmetric unit probably contains one molecule, with a solvent content of 43.6%. ..
  85. Tomono A, Mashiko M, Shimazu T, Inoue H, Nagasawa H, Yoshida M, et al. Self-activation of serine/threonine kinase AfsK on autophosphorylation at threonine-168. J Antibiot (Tokyo). 2006;59:117-23 pubmed
    ..All these findings show that autophosphorylation or intermolecular phosphorylation of threonine-168 in AfsK accounts for the self-activation of its kinase activity. ..
  86. Xu Y, Liao C, Yao L, Ye X, Ye B. GlnR and PhoP Directly Regulate the Transcription of Genes Encoding Starch-Degrading, Amylolytic Enzymes in Saccharopolyspora erythraea. Appl Environ Microbiol. 2016;82:6819-6830 pubmed publisher
  87. Wohlleben W, Alijah R, Dorendorf J, Hillemann D, Nussbaumer B, Pelzer S. Identification and characterization of phosphinothricin-tripeptide biosynthetic genes in Streptomyces viridochromogenes. Gene. 1992;115:127-32 pubmed
    ..The previously described PTT resistance-encoding gene (pat) was located between the phsA and the dea genes...
  88. Allen K, Lavie A, Glasfeld A, Tanada T, Gerrity D, Carlson S, et al. Role of the divalent metal ion in sugar binding, ring opening, and isomerization by D-xylose isomerase: replacement of a catalytic metal by an amino acid. Biochemistry. 1994;33:1488-94 pubmed
    ..7%, respectively. The wild-type and both E180K structures show no significant structural differences, except the epsilon-amino group of Lys180, which occupies the position usually occupied by the Mg-1.(ABSTRACT TRUNCATED AT 250 WORDS)..
  89. Cho Y, Roe J. Isolation and expression of the catA gene encoding the major vegetative catalase in Streptomyces coelicolor Müller. J Bacteriol. 1997;179:4049-52 pubmed
    ..catA expression was increased by H2O2 treatment but did not increase during stationary phase. A putative catalase (CatB) cross-reactive with anti-CatA antibody appeared during stationary phase and in the aerial mycelium...
  90. Blanco G, Fern ndez E, Fern ndez M, Bra a A, Weissbach U, K nzel E, et al. Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus. Mol Gen Genet. 2000;262:991-1000 pubmed
    ..These experiments demonstrate that the glycosyltransferases MtmGIV and MtmGIII catalyze the first two glycosylation steps in mithramycin biosynthesis. A model is proposed for the glycosylation steps in mithramycin biosynthesis...
  91. Mark B, Vocadlo D, Knapp S, Triggs Raine B, Withers S, James M. Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase. J Biol Chem. 2001;276:10330-7 pubmed publisher
    ..This complex provides decisive structural evidence for substrate-assisted catalysis and the formation of a covalent, cyclic intermediate in family 20 beta-hexosaminidases...
  92. Schaerlaekens K, Schierov M, Lammertyn E, Geukens N, Ann J, Van Mellaert L. Twin-arginine translocation pathway in Streptomyces lividans. J Bacteriol. 2001;183:6727-32 pubmed publisher
    ..the Tat pathway is functional. Moreover, this Tat pathway can translocate folded proteins, and the E. coli TorA signal peptide can direct Tat-dependent transport in S. lividans...