Streptomyces venezuelae

Summary

Alias: Streptomyces venezuelensis, Streptomyces venezuelae Ehrlich et al. 1948

Top Publications

  1. ncbi Site-specific integration of bacteriophage VWB genome into Streptomyces venezuelae and construction of a VWB-based integrative vector
    L Van Mellaert
    Laboratory of Bacteriology, Rega Instituut, KU Leuven, Belgium
    Microbiology 144:3351-8. 1998
  2. ncbi Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae
    Won Seok Jung
    Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56 1, Shilim Dong, Gwanak Gu, Seoul 151 742, South Korea
    Appl Microbiol Biotechnol 76:1373-81. 2007
  3. ncbi Modular architecture of the conjugative plasmid pSVH1 from Streptomyces venezuelae
    Jens Reuther
    Microbiology Biotechnology, Microbiological Institute, Faculty of Biology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Plasmid 55:201-9. 2006
  4. doi Three-dimensional structure of DesVI from Streptomyces venezuelae: a sugar N,N-dimethyltransferase required for dTDP-desosamine biosynthesis
    E Sethe Burgie
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 47:3982-8. 2008
  5. doi Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
    Sushila Maharjan
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Tangjeonmyun, Asansi, Chungnam 336 708, Republic of Korea
    Biotechnol Lett 32:277-82. 2010
  6. pmc Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine
    E Sethe Burgie
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 46:8999-9006. 2007
  7. ncbi Heterologous production and detection of recombinant directing 2-deoxystreptamine (DOS) in the non-aminoglycoside-producing host Streptomyces venezuelae YJ003
    Nagendra Prasad Kurumbang
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asan 336 708, Korea
    J Microbiol Biotechnol 18:866-73. 2008
  8. ncbi Genetically engineered biosynthesis of macrolide derivatives including 4-amino-4,6-dideoxy-L-glucose from Streptomyces venezuelae YJ003-OTBP3
    Binod Babu Pageni
    Institute of Biomolecule Reconstruction iBR, Department of Pharmaceutical Engineering, SunMoon University, Asan 336 708, Korea
    J Microbiol Biotechnol 18:88-94. 2008
  9. pmc Expression, purification and preliminary diffraction studies of CmlS
    Ryan Latimer
    Department of Chemistry, Queen s University, Kingston, Ontario, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:260-3. 2009
  10. ncbi Complete genomic nucleotide sequence and analysis of the temperate bacteriophage VWB
    W Van Dessel
    Laboratorium voor Bacteriologie, Katholieke Universiteit Leuven, Rega Instituut, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Virology 331:325-37. 2005

Scientific Experts

  • David L Jakeman
  • T Izard
  • Matthew Bush
  • Gang Liu
  • Mark W Ruszczycky
  • Maureen J Bibb
  • David Sherman
  • Feng Gao
  • S Pospisil
  • Bin Hong
  • E Lammertyn
  • Yeo Joon Yoon
  • Koji Yanai
  • Je Won Park
  • Amy Wright
  • Sung Ryeol Park
  • Ah Reum Han
  • Jae Kyung Sohng
  • CHARLES SIH
  • WALTON BYRNES
  • Yongquan Xue
  • Eun Ji Kim
  • Yeon Hee Ban
  • Won Seok Jung
  • Young Ji Yoo
  • Keqian Yang
  • Sushila Maharjan
  • Eunji Kim
  • Tae Jin Oh
  • E Sethe Burgie
  • PAUL SHELDON
  • WALTON MALCOLM BYRNES
  • Peter McCarthy
  • Charles Hutchinson
  • WILLIAM GERWICK
  • Kwangkyoung Liou
  • Hei Chan Lee
  • Balakrishnan Naveena
  • Huarong Tan
  • Juan Wang
  • Linqi Wang
  • Nagendra Prasad Kurumbang
  • Binod Babu Pageni
  • Jay Sung Joong Hong
  • Pramod B Shinde
  • Svetlana A Borisova
  • Byung Gee Kim
  • Keqiang Fan
  • Kateryna Podzelinska
  • Gangming Xu
  • Ryan Latimer
  • Walter E Kowtoniuk
  • Leo C Vining
  • Jeffrey D Kittendorf
  • Hung wen Liu
  • Cha Yong Choi
  • Hazel M Holden
  • Jens Reuther
  • Marta Santos
  • Steven J Malcolmson
  • Margaret C M Smith
  • Yuanyuan Pan
  • Julio C Ayala
  • So Ra Lee
  • Nagarajan Partha
  • Dong Gun Lee
  • Jaeyong Cho
  • Yang Shen
  • Rong Ding
  • Uwe Rix
  • Steven T Pullan
  • Yongchang Ouyang
  • Stephanie N Dupuis
  • Mi Sun Ahn
  • Giorgia Letizia Marcone
  • Yuki Goto
  • Xiuyun Tian
  • Anupam Bhattacharya
  • Zongchao Jia
  • David L Zechel
  • Ji Hye Paik
  • Haihua Yang
  • Shengying Li
  • Y Grace Chen
  • Sailesh Malla
  • L C Vining
  • Jia Li
  • David R Liu
  • Isha Agarwal
  • Yinghua Shen

Detail Information

Publications98

  1. ncbi Site-specific integration of bacteriophage VWB genome into Streptomyces venezuelae and construction of a VWB-based integrative vector
    L Van Mellaert
    Laboratory of Bacteriology, Rega Instituut, KU Leuven, Belgium
    Microbiology 144:3351-8. 1998
    The temperate bacteriophage VWB integrates into the chromosome of Streptomyces venezuelae ETH14630 via site-specific integration...
  2. ncbi Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae
    Won Seok Jung
    Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56 1, Shilim Dong, Gwanak Gu, Seoul 151 742, South Korea
    Appl Microbiol Biotechnol 76:1373-81. 2007
    To develop a system for combinatorial biosynthesis of glycosylated macrolides, Streptomyces venezuelae was genetically manipulated to be deficient in the production of its macrolide antibiotics by deletion of the entire biosynthetic gene ..
  3. ncbi Modular architecture of the conjugative plasmid pSVH1 from Streptomyces venezuelae
    Jens Reuther
    Microbiology Biotechnology, Microbiological Institute, Faculty of Biology, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Plasmid 55:201-9. 2006
    The conjugative rolling circle replication (RCR) type plasmid pSVH1 from the chloramphenicol producer Streptomyces venezuelae was characterized by DNA sequence analysis and insertion/deletion analysis...
  4. doi Three-dimensional structure of DesVI from Streptomyces venezuelae: a sugar N,N-dimethyltransferase required for dTDP-desosamine biosynthesis
    E Sethe Burgie
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 47:3982-8. 2008
    ..Six enzymes are required for the biosynthesis of dTDP-desosamine in Streptomyces venezuelae, with the last step catalyzed by DesVI, an N, N-dimethyltransferase...
  5. doi Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
    Sushila Maharjan
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Tangjeonmyun, Asansi, Chungnam 336 708, Republic of Korea
    Biotechnol Lett 32:277-82. 2010
    Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides...
  6. pmc Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine
    E Sethe Burgie
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 46:8999-9006. 2007
    ..In the bacterium Streptomyces venezuelae, six enzymes are required for the production of dTDP-desosamine...
  7. ncbi Heterologous production and detection of recombinant directing 2-deoxystreptamine (DOS) in the non-aminoglycoside-producing host Streptomyces venezuelae YJ003
    Nagendra Prasad Kurumbang
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asan 336 708, Korea
    J Microbiol Biotechnol 18:866-73. 2008
    ..The recombinant pNDOS was transformed into a non-aminoglycoside-producing host, Streptomyces venezuelae YJ003, for heterologous expression...
  8. ncbi Genetically engineered biosynthesis of macrolide derivatives including 4-amino-4,6-dideoxy-L-glucose from Streptomyces venezuelae YJ003-OTBP3
    Binod Babu Pageni
    Institute of Biomolecule Reconstruction iBR, Department of Pharmaceutical Engineering, SunMoon University, Asan 336 708, Korea
    J Microbiol Biotechnol 18:88-94. 2008
    ..These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar...
  9. pmc Expression, purification and preliminary diffraction studies of CmlS
    Ryan Latimer
    Department of Chemistry, Queen s University, Kingston, Ontario, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:260-3. 2009
    CmlS, a flavin-dependent halogenase (FDH) present in the chloramphenicol-biosynthetic pathway in Streptomyces venezuelae, directs the dichlorination of an acetyl group...
  10. ncbi Complete genomic nucleotide sequence and analysis of the temperate bacteriophage VWB
    W Van Dessel
    Laboratorium voor Bacteriologie, Katholieke Universiteit Leuven, Rega Instituut, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Virology 331:325-37. 2005
    The entire double-stranded DNA genome of the Streptomyces venezuelae bacteriophage VWB was sequenced and analyzed. Its size is 49,220 bp with an overall molar G + C content of 71.2 mol%...
  11. pmc Genes required for aerial growth, cell division, and chromosome segregation are targets of WhiA before sporulation in Streptomyces venezuelae
    Matthew J Bush
    Department of Molecular Microbiology, John Innes Centre, Norwich, United Kingdom
    MBio 4:e00684-13. 2013
    ..ChIP-seq) and microarray transcriptional profiling, exploiting a new model organism for the genus, Streptomyces venezuelae, which sporulates in liquid culture...
  12. ncbi Inactivation of the 20S proteasome in Streptomyces lividans and its influence on the production of heterologous proteins
    Bin Hong
    Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical University, No 1 Tiantanxili, Beijing 100050, China
    Microbiology 151:3137-45. 2005
    ..Using the expression and secretion signals of the subtilisin inhibitor of Streptomyces venezuelae CBS762...
  13. pmc Functional analyses of oxygenases in jadomycin biosynthesis and identification of JadH as a bifunctional oxygenase/dehydrase
    Yi Hua Chen
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences and Graduate School of Chinese Academy of Sciences, Beijing 100080, China
    J Biol Chem 280:22508-14. 2005
    A novel angucycline metabolite, 2,3-dehydro-UWM6, was identified in a jadH mutant of Streptomyces venezuelae ISP5230. Both UWM6 and 2,3-dehydro-UWM6 could be converted to jadomycin A or B by a ketosynthase alpha (jadA) mutant of S...
  14. ncbi Construction of ivermectin producer by domain swaps of avermectin polyketide synthase in Streptomyces avermitilis
    Xiaolin Zhang
    Academy of State Administration of Grain, No 11 Baiwanzhuang Avenue, Beijing, 100037, PR China
    Appl Microbiol Biotechnol 72:986-94. 2006
    ..the DNA fragment encoding the DH, enoylreductase, and KR domains from module 4 of the pikromycin PKS of Streptomyces venezuelae ATCC 15439 using a gene replacement vector pXL211...
  15. ncbi Bacterial P450-catalyzed polyketide hydroxylation on a microfluidic platform
    Aravind Srinivasan
    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Biotechnol Bioeng 88:528-35. 2004
    ..The PikC hydroxylase Streptomyces venezuelae was incorporated into a PDMS-based microfluidic channel...
  16. ncbi Comparison of the Sec and Tat secretion pathways for heterologous protein production by Streptomyces lividans
    Kristien Schaerlaekens
    Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Biotechnol 112:279-88. 2004
    ..lower for Tat-dependent than for Sec-dependent transport using the Sec-dependent signal peptide of the Streptomyces venezuelae subtilisin inhibitor. Surprisingly, Sec-dependent transport was enhanced in the tatB deletion strain...
  17. ncbi Structural basis for macrolactonization by the pikromycin thioesterase
    David L Akey
    Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109 2216, USA
    Nat Chem Biol 2:537-42. 2006
    ..of macrolactone ring formation catalyzed by the pikromycin polyketide synthase thioesterase domain from Streptomyces venezuelae. A pentaketide phosphonate mimic of the final pikromycin linear chain-elongation intermediate was ..
  18. ncbi Total synthesis of narbonolide and biotransformation to pikromycin
    Lakshmanan Venkatraman
    Department of Medicinal Chemistry, 8 101 WDH, University of Minnesota, Minneapolis, Minnesota 55455 0353, USA
    J Org Chem 71:9853-6. 2006
    ..A pikAI deletion mutant of Streptomyces venezuelae was used to biotransform synthetic narbonolide to pikromycin by glycosylation and oxidation in vivo...
  19. ncbi The dynamic structure of jadomycin B and the amino acid incorporation step of its biosynthesis
    Uwe Rix
    Division of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 907 Rose Street, Lexington, Kentucky 40536 0082, USA
    J Am Chem Soc 126:4496-7. 2004
    ..with a unique 8H-benz[b]oxazolo[3,2-f]phenanthridine pentacyclic skeleton produced by the bacterium Streptomyces venezuelae ISP 5230, exists in a dynamic equilibrium of two diastereomers differing in the configuration of C-3a...
  20. pmc Evolutionary relationships among actinophages and a putative adaptation for growth in Streptomyces spp
    Margaret C M Smith
    Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom
    J Bacteriol 195:4924-35. 2013
    ..e., use of the rare UUA codon, to control translation of the essential phage protein, the terminase. The Streptomyces venezuelae generalized transducing phage SV1 was used to predict the presence of other generalized transducing ..
  21. ncbi The hidden steps of domain skipping: macrolactone ring size determination in the pikromycin modular polyketide synthase
    Brian J Beck
    Department of Microbiology and Biotechnology Institute, University of Minnesota, Mayo Mail Code 196, 420 Delaware Street S E, Minneapolis, MN 55455, USA
    Chem Biol 9:575-83. 2002
    The pikromycin (Pik) polyketide synthase (PKS) from Streptomyces venezuelae comprises four multifunctional polypeptides (PikAI, PikAII, PikAIII, and PikAIV)...
  22. doi Combinatorial biosynthesis and antibacterial evaluation of glycosylated derivatives of 12-membered macrolide antibiotic YC-17
    Pramod B Shinde
    Department of Chemistry and Nano Sciences, Ewha Womans University, Seoul 120 750, Republic of Korea Institute of Nano Biotechnology, Ewha Womans University, Seoul 120 750, Republic of Korea
    J Biotechnol 168:142-8. 2013
    ..the gene encoding a substrate-flexible glycosyltransferase DesVII were constructed and introduced into Streptomyces venezuelae YJ003 mutant strain bearing a deletion of a desosamine biosynthetic (des) gene cluster...
  23. ncbi Generation of multiple bioactive macrolides by hybrid modular polyketide synthases in Streptomyces venezuelae
    Yeo Joon Yoon
    Department of Microbiology and Biotechnology Institute, University of Minnesota, Mayo Mail Code 196, 1460 Mayo Memorial Building, Minneapolis, MN 55455, USA
    Chem Biol 9:203-14. 2002
    ..This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products...
  24. ncbi Characterization of the glycosyltransferase activity of desVII: analysis of and implications for the biosynthesis of macrolide antibiotics
    Svetlana A Borisova
    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    J Am Chem Soc 126:6534-5. 2004
    ..involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described...
  25. doi Engineered biosynthesis of glycosylated derivatives of narbomycin and evaluation of their antibacterial activities
    Ah Reum Han
    Interdisciplinary Programs of Bioengineering, Seoul National University, Seoul 151 742, Republic of Korea
    Appl Microbiol Biotechnol 93:1147-56. 2012
    A 14-membered macrolide antibiotic narbomycin produced from Streptomyces venezuelae ATCC 15439 is composed of polyketide macrolactone ring and D-desosamine as a deoxysugar moiety, which acts as an important determinant of its ..
  26. pmc Disruption of rimP-SC, encoding a ribosome assembly cofactor, markedly enhances the production of several antibiotics in Streptomyces coelicolor
    Yuanyuan Pan
    Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    Microb Cell Fact 12:65. 2013
    ..the close relationship between protein synthesis and the production of secondary metabolites, the function of ribosome assembly cofactor RimP on antibiotics production was studied in Streptomyces coelicolor and Streptomyces venezuelae.
  27. doi Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans
    Julio C Ayala
    Institute of Pharmacy and Food, University of Havana, Havana, Cuba
    J Microbiol Methods 94:192-8. 2013
    ..protein 1), were equipped with a 3'-Strep-tag® II-encoding sequence and placed under control of the Streptomyces venezuelae CBS762.70 subtilisin inhibitor (vsi) transcriptional, translational and signal sequences...
  28. pmc The posttranslational modification cascade to the thiopeptide berninamycin generates linear forms and altered macrocyclic scaffolds
    Steven J Malcolmson
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:8483-8. 2013
    ..from Streptomyces bernensis UC 5144, its heterologous expression in Streptomyces lividans TK24 and Streptomyces venezuelae ATCC 10712, and detection of variant and incompletely processed scaffolds...
  29. doi An efficacious degradation of pesticide by salt tolerant Streptomyces venezuelae ACT 1
    Balakrishnan Naveena
    Department of Chemical Engineering, AC College of Technology, Anna University Chennai, Chennai 600 025, India
    Bioresour Technol 132:378-82. 2013
    Degradation of the organophosphorus pesticide has been studied using the marine isolate, Streptomyces venezuelae ACT1. The organism exhibited a specific growth rate of 0.371h(-1) and the organophosphorus hydrolase activity rate as 0...
  30. pmc Heterologous production of 4-O-demethylbarbamide, a marine cyanobacterial natural product
    Eun Ji Kim
    Department of Chemistry and Nano Sciences, Ewha Womans University, Seoul 120 750, Republic of Korea
    Org Lett 14:5824-7. 2012
    ..cluster, obtained from the marine cyanobacterium Moorea producens, in the terrestrial actinobacterium Streptomyces venezuelae, resulted in the production of a new barbamide congener 4-O-demethylbarbamide, demonstrating the ..
  31. doi New methymycin derivatives of Streptomyces venezuelae ATCC 15439 and their inhibitory effects on human T cell proliferation mediated by PMA/ionomycin
    Rong Ding
    College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China
    Arch Pharm Res 35:1567-72. 2012
    ..and 3'-demethyldeoxymethymycin (2), together with seven known ones (3-9), were obtained from the strain Streptomyces venezuelae ATCC 15439. Their structures were determined on the basis of IR, MS, 1D and 2D NMR data...
  32. doi Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01)
    Yang Shen
    School of Biotechnology, East China University of Science and Technology, Meilong Rd 130, Shanghai, People s Republic of China
    Biotechnol Lett 34:2087-91. 2012
    ..Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A...
  33. doi Enhanced production of thrombinase by Streptomyces venezuelae: kinetic studies on growth and enzyme production of mutant strain
    Balakrishnan Naveena
    Department of Chemical Engineering, A C College of Technology, Anna University Chennai, Chennai 600 025, India
    Bioresour Technol 111:417-24. 2012
    This investigation provides the enhanced production of thrombinase, a fibrinolytic enzyme using mutant Streptomyces venezuelae. Initially the mutagenesis of the marine isolate was done by UV and Ethyl methane sulfonate (EMS) and their ..
  34. doi Polyester hydrolytic and synthetic activity catalyzed by the medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces venezuelae SO1
    Marta Santos
    Enzyme and Cell Technology Group, Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country UPV EHU, P O Box 644, 48080, Bilbao, Spain
    Appl Microbiol Biotechnol 97:211-22. 2013
    ..medium-chain-length polyhydroxyalkanote (MCL-PHA) depolymerase from an isolate identified as Streptomyces venezuelae SO1 was purified to electrophoretic homogeneity and characterized...
  35. pmc Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator
    Linqi Wang
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People s Republic of China
    Proc Natl Acad Sci U S A 106:8617-22. 2009
    ..However, such mechanisms have not been established. JadR1, an OmpR-type ARR of Streptomyces venezuelae, appears to activate the transcription of jadomycin B (JdB) biosynthetic genes while repressing its own ..
  36. doi Enhancement of doxorubicin production by expression of structural sugar biosynthesis and glycosyltransferase genes in Streptomyces peucetius
    Sailesh Malla
    Institute of Biomolecule Reconstruction iBR, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan ri, Tangjeong myeon, Asansi, Chungnam 336 708, Republic of Korea
    J Biosci Bioeng 108:92-8. 2009
    To enhance doxorubicin (DXR) production, the structural sugar biosynthesis genes desIII and desIV from Streptomyces venezuelae ATCC 15439 and the glycosyltransferase pair dnrS/dnrQ from Streptomyces peucetius ATCC 27952 were cloned into ..
  37. pmc Discovery of unique lanthionine synthetases reveals new mechanistic and evolutionary insights
    Yuki Goto
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois, United States of America
    PLoS Biol 8:e1000339. 2010
    ..of three distinct catalytic domains and demonstrate in vitro enzyme activity of a family member from Streptomyces venezuelae. Analysis of individually expressed and purified domains shows that LanL enzymes install dehydroamino ..
  38. doi Engineering of plant-specific phenylpropanoids biosynthesis in Streptomyces venezuelae
    Sung Ryeol Park
    Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Republic of Korea
    J Biotechnol 141:181-8. 2009
    ..To expand the applicability of Streptomyces venezuelae as a heterologous host to plant polyketide production, flavonoid and stilbene biosynthetic genes were ..
  39. pmc Novel mechanism of glycopeptide resistance in the A40926 producer Nonomuraea sp. ATCC 39727
    Giorgia Letizia Marcone
    Dipartimento di Biotecnologie e Scienze Molecolari, Universita degli Studi dell Insubria, Varese, Italy
    Antimicrob Agents Chemother 54:2465-72. 2010
    ..Heterologous expression of vanY in a sensitive Streptomyces species, Streptomyces venezuelae, resulted in higher levels of glycopeptide resistance.
  40. pmc Stoichiometry of the redox neutral deamination and oxidative dehydrogenation reactions catalyzed by the radical SAM enzyme DesII
    Mark W Ruszczycky
    Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
    J Am Chem Soc 132:2359-69. 2010
    DesII from Streptomyces venezuelae is a radical SAM (S-adenosyl-l-methionine) enzyme that catalyzes the deamination of TDP-4-amino-4,6-dideoxy-d-glucose to form TDP-3-keto-4,6-dideoxy-d-glucose in the biosynthesis of TDP-d-desosamine...
  41. ncbi [Evaluation of the activities of two promoters in Streptomycetes by reporter gene method]
    Jia Li
    School of Life Sciences, Capital Normal University, Beijing 100048, China
    Wei Sheng Wu Xue Bao 49:1454-8. 2009
    ..The mutated promoter of the erythromycin resistance gene (PermE *) is a strong promoter generally used in streptomycetes, and we evaluated the expression activities of a new promoter (Psf) and PermE * in Streptomycetes...
  42. pmc Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine
    Ping Hui Szu
    Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
    J Am Chem Soc 131:14030-42. 2009
    ..antibiotics including methymycin (2), neomethymycin (3), pikromycin (4), and narbomycin (5) produced by Streptomyces venezuelae . It plays an essential role in conferring biological activities to its parent aglycones...
  43. pmc LC/MS analysis of cellular RNA reveals NAD-linked RNA
    Y Grace Chen
    Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Chem Biol 5:879-81. 2009
    ..This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro ..
  44. doi Chloramphenicol biosynthesis: the structure of CmlS, a flavin-dependent halogenase showing a covalent flavin-aspartate bond
    Kateryna Podzelinska
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Mol Biol 397:316-31. 2010
    ..The operon for chloramphenicol biosynthesis in Streptomyces venezuelae encodes the chloramphenicol halogenase CmlS, which belongs to the large and diverse family of flavin-..
  45. ncbi [Construction of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and the expression of vitreoscilla hemoglobin]
    Feng Gao
    College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A and F University, Yangling 712100, China
    Wei Sheng Wu Xue Bao 50:406-10. 2010
    To Construct of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and express of vitreoscilla hemoglobin.
  46. pmc "Pseudo" gamma-butyrolactone receptors respond to antibiotic signals to coordinate antibiotic biosynthesis
    Gangming Xu
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    J Biol Chem 285:27440-8. 2010
    ..Likewise, JadR2 is also a putative GBL receptor homologue in Streptomyces venezuelae, the producer of chloramphenicol and cryptic antibiotic jadomycin...
  47. ncbi Enhanced flavonoid production in Streptomyces venezuelae via metabolic engineering
    Sung Ryeol Park
    Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120 750, Korea
    J Microbiol Biotechnol 21:1143-6. 2011
    ..Recently, we have developed a recombinant Streptomyces venezuelae as a heterologous host for the production of flavonoids...
  48. doi Jadomycins derived from the assimilation and incorporation of norvaline and norleucine
    Stephanie N Dupuis
    Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada
    J Nat Prod 74:2420-4. 2011
    b>Streptomyces venezuelae ISP5230 is recognized for the production of chloramphenicol and the jadomycin family of natural products...
  49. doi Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation
    Je Won Park
    Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, Republic of Korea
    Nat Chem Biol 7:843-52. 2011
    ..of putative biosynthetic genes from Streptomyces kanamyceticus in the non-aminoglycoside-producing Streptomyces venezuelae. Unexpectedly, we discovered that the biosynthetic pathway contains an early branch point, governed by ..
  50. doi A novel role of 'pseudo'γ-butyrolactone receptors in controlling γ-butyrolactone biosynthesis in Streptomyces
    Juan Wang
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Mol Microbiol 82:236-50. 2011
    ..Similarly, the pseudo GBL receptor JadR2 in Streptomyces venezuelae was shown to repress the expression of jadW1, which also encodes the putative GBL synthase...
  51. ncbi Biosynthesis of glycosylated derivatives of tylosin in Streptomyces venezuelae
    Ah Reum Han
    Interdisciplinary Programs of Bioengineering, Seoul National University, Seoul, Korea
    J Microbiol Biotechnol 21:613-6. 2011
    b>Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis ..
  52. pmc Development of a Streptomyces venezuelae-based combinatorial biosynthetic system for the production of glycosylated derivatives of doxorubicin and its biosynthetic intermediates
    Ah Reum Han
    Interdisciplinary Programs of Bioengineering, Seoul National University, Seoul 151 742, Republic of Korea
    Appl Environ Microbiol 77:4912-23. 2011
    ..or its biosynthetic intermediates, rhodomycin D and daunorubicin, was developed through the use of Streptomyces venezuelae mutants carrying plasmids that direct the biosynthesis of different nucleotide deoxysugars and their ..
  53. pmc Mechanistic studies of the radical S-adenosyl-L-methionine enzyme DesII: EPR characterization of a radical intermediate generated during its catalyzed dehydrogenation of TDP-D-quinovose
    Mark W Ruszczycky
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, Texas 78712, USA
    J Am Chem Soc 133:7292-5. 2011
    DesII, a radical S-adenosyl-l-methionine (SAM) enzyme from Streptomyces venezuelae, catalyzes the deamination of TDP-4-amino-4,6-dideoxy-D-glucose to TDP-3-keto-4,6-dideoxy-D-glucose in the desosamine biosynthetic pathway...
  54. doi Microbial transformation of trichostatin A to 2,3-dihydrotrichostatin A
    Je Won Park
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Chungnam 336 708, Republic of Korea
    J Nat Prod 74:1272-4. 2011
    ..2,3-dihydrotrichostatin A, was created from trichostatin A by employing a recombinant strain of Streptomyces venezuelae as a microbial catalyst...
  55. pmc Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes
    Steven T Pullan
    Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK
    BMC Genomics 12:175. 2011
    ..We applied a global in vivo approach to identify the GlnR regulon of Streptomyces venezuelae, which, unlike many actinomycetes, grows in a diffuse manner that is suitable for physiological studies...
  56. doi A method to type the potential angucycline producers in actinomycetes isolated from marine sponges
    Yongchang Ouyang
    Key Laboratory of Marine Bio resources Sustainable Utilization LMB CAS, Guangdong Key Laboratory of Marine Materia Medica LMMM GD, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People s Republic of China
    Antonie Van Leeuwenhoek 99:807-15. 2011
    ..of angucycline, were designed and evaluated in a PCR protocol targeting the jadomycin cyclase gene of Streptomyces venezuelae ISP5230. The identity of the target amplicon was confirmed by sequencing...
  57. ncbi Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae
    Sung Ryeol Park
    Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120 750, Korea
    J Microbiol Biotechnol 20:1295-9. 2010
    Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides...
  58. doi Biosynthesis of ribostamycin derivatives by reconstitution and heterologous expression of required gene sets
    Nagendra Prasad Kurumbang
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, SunMoon University, 100 Kalsan ri, Tangjeong myeon, Asan, Chungnam, Republic of Korea
    Appl Biochem Biotechnol 163:373-82. 2011
    ..biosynthesis of 6'-deamino-6'-hydroxyribostamycin (a ribostamycin derivative or pseudoribostamycin) in Streptomyces venezuelae YJ003 by reconstructing gene cassettes for direct ribosylation of paromamine...
  59. pmc Characterization of glycosyltransferase DesVII and its auxiliary partner protein DesVIII in the methymycin/picromycin biosynthetic pathway
    Svetlana A Borisova
    Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    Biochemistry 49:8071-84. 2010
    ..in the biosynthesis of the macrolide antibiotics methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described...
  60. pmc A chemical screen for biological small molecule-RNA conjugates reveals CoA-linked RNA
    Walter E Kowtoniuk
    Howard Hughes Medical Institute and Department of Chemistry and Chemical Biology, 12 Oxford Street, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:7768-73. 2009
    ..Application to Escherichia coli and Streptomyces venezuelae RNA revealed an RNA-linked hydroxyfuranone or succinyl ester group, in addition to a number of other ..
  61. pmc Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine
    E Sethe Burgie
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Protein Sci 16:887-96. 2007
    ..Six enzymes are required for its biosynthesis in Streptomyces venezuelae. The focus of this article is DesV, which catalyzes the PLP-dependent replacement of a 3-keto group with ..
  62. ncbi Functional analysis of desVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation
    Jay Sung Joong Hong
    Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, San 56 1, Shilim Dong, Gwanak Gu, Seoul 151 742, Republic of Korea
    Gene 386:123-30. 2007
    ..in the biosynthesis of macrolide antibiotics, neomethymycin, methymycin and pikromycin, in Streptomyces venezuelae ATCC 15439...
  63. ncbi Streptomyces gulbargensis sp. nov., isolated from soil in Karnataka, India
    Syed G Dastager
    Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650 091, P R China
    Antonie Van Leeuwenhoek 91:99-104. 2007
    ..16S rRNA gene sequence analysis revealed that strain DAS 131T was most closely related to Streptomyces venezuelae ISP 5230T (AY999739) with a sequence similarity of 99.5% (8 nucleotide differences out of 1,477)...
  64. pmc The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae
    David H Sherman
    Life Sciences Institute and Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA
    J Biol Chem 281:26289-97. 2006
    The pikromycin (Pik)/methymycin biosynthetic pathway of Streptomyces venezuelae represents a valuable system for dissecting the fundamental mechanisms of modular polyketide biosynthesis, aminodeoxysugar assembly, glycosyltransfer, and ..
  65. ncbi Novel and expanded jadomycins incorporating non-proteogenic amino acids
    David L Jakeman
    College of Pharmacy, Dalhousie University, 5968 College Street, Halifax, B3H 3J5, NS, Canada
    Bioorg Med Chem Lett 15:5280-3. 2005
    Jadomycin B is a secondary metabolite produced, in response to stress, by Streptomyces venezuelae ISP5230 grown in nutrient-deprived media...
  66. ncbi Novel jadomycins: incorporation of non-natural and natural amino acids
    David L Jakeman
    College of Pharmacy, Dalhousie University, 5968 College Street, Halifax, Nova Scotia, B3H 3J5, Canada
    Bioorg Med Chem Lett 15:1447-9. 2005
    Electrospray ionization mass spectrometry of extracts from Streptomyces venezuelae ISP5230 cultures grown on chemically synthesized non-natural L-amino acids, D-amino acids or any of the 20 natural amino acids demonstrated incorporation ..
  67. ncbi Para-position derivatives of fungal anthelmintic cyclodepsipeptides engineered with Streptomyces venezuelae antibiotic biosynthetic genes
    Koji Yanai
    Microbiological Resources and Technology Laboratories, Meiji Seika Kaisha, Ltd, 788 Kayama, Odawara shi, Kanagawa 250 0852, Japan
    Nat Biotechnol 22:848-55. 2004
    ..PF1022. Three genes cloned from Streptomyces venezuelae, and required for the biosynthesis of p-aminophenylpyruvate from chorismate in the chloramphenicol ..
  68. ncbi Culture conditions improving the production of jadomycin B
    David L Jakeman
    College of Pharmacy, Dalhousie University, Halifax, NS, Canada
    J Ind Microbiol Biotechnol 33:767-72. 2006
    The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection...
  69. ncbi Biosynthesis of sulfur-containing amino acids in Streptomyces venezuelae ISP5230: roles for cystathionine beta-synthase and transsulfuration
    Z Chang
    Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1
    Microbiology 148:2135-47. 2002
    A 0.5 kb fragment of Streptomyces venezuelae ISP5230 genomic DNA was amplified by PCR using primers based on consensus sequences of cysteine synthase isozyme A from bacteria...
  70. ncbi Oxidation of lincomycin by hydrogen peroxide restricts its potential biotransformation with haloperoxidases
    S Pospisil
    Institute of Microbiology, Academy of Sciences of the Czech Republic, 124 20 Prague, Czechia
    Folia Microbiol (Praha) 46:376-8. 2001
    Lincomycin biotransformation was conducted by using Streptomyces venezuelae and Streptomyces phaeochromogenes cell-free extracts...
  71. pmc Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity
    T Izard
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105 2794, USA
    Protein Sci 10:1508-13. 2001
    b>Streptomyces venezuelae synthesizes chloramphenicol (Cm), an inhibitor of ribosomal peptidyl transferase activity, thereby inhibiting bacterial growth...
  72. pmc The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 19:2690-700. 2000
    Chloramphenicol (Cm), produced by the soil bacterium Streptomyces venezuelae, is an inhibitor of bacterial ribosomal peptidyltransferase activity...
  73. ncbi An acyl-coenzyme A carboxylase encoding gene associated with jadomycin biosynthesis in Streptomyces venezuelae ISP5230
    L Han
    Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    Microbiology 146:903-10. 2000
    ..region of chromosomal DNA lying between jadR1 and jadI in the gene cluster for jadomycin biosynthesis in Streptomyces venezuelae ISP5230 detected an ORF encoding 584 amino acids similar in sequence to the biotin carboxylase (BC) and ..
  74. ncbi Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces venezuelae: evidence that it is not required for chlorination in chloramphenicol biosynthesis
    S J Facey
    Institut fur Mikrobiologie, Universitat Hohenheim, Stuttgart, Germany
    Microbiology 142:657-65. 1996
    Genomic DNA libraries of Streptomyces venezuelae ISP5230 and of a mutant blocked at the chlorination step of chloramphenicol biosynthesis were probed by hybridization with a synthetic oligonucleotide corresponding to the N-terminal amino ..
  75. ncbi A role for pabAB, a p-aminobenzoate synthase gene of Streptomyces venezuelae ISP5230, in chloramphenicol biosynthesis
    M P Brown
    Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    Microbiology 142:1345-55. 1996
    Mutagenesis of Streptomyces venezuelae ISP5230 and selection for P-aminobenzoic acid-dependent growth in the presence of sulfanilamide yielded pab mutants (VS519 and VS620) that continued to produce chloramphenicol (Cm), although with ..
  76. ncbi Cloning and sequence analysis of a recA-like gene from Streptomyces venezuelae ISP5230
    W Yao
    Biology Department, Dalhousie University, Halifax, Nova Scotia, Canada
    FEMS Microbiol Lett 118:51-6. 1994
    When genomic DNA fragments from Streptomyces venezuelae ISP5230 were probed at moderate stringency with recA from Mycobacterium tuberculosis, a 2.0-kb SmaI fragment was identified. The fragment was isolated by cloning a BamHI digest of S...
  77. ncbi Analysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry
    Je Won Park
    Division of Nano Sciences and Department of Chemistry, Ewha Womans University, Seoul, Republic of Korea
    J Mass Spectrom 42:1136-47. 2007
    ..metabolomic study is demonstrated by the profiling of the intracellular CoA-ester pools in the wild-type Streptomyces venezuelae strain producing polyketide antibiotics (methymycin and pikromycin), a polyketide synthase (PKS)-deleted ..
  78. ncbi Engineering a regulatory region of jadomycin gene cluster to improve jadomycin B production in Streptomyces venezuelae
    Jian Ting Zheng
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, P O Box 2714, Beijing, 100081, People s Republic of China
    Appl Microbiol Biotechnol 76:883-8. 2007
    b>Streptomyces venezuelae ISP5230 produces a group of jadomycin congeners with cytotoxic activities. To improve jadomycin fermentation process, a genetic engineering strategy was designed to replace a 3...
  79. ncbi Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003
    Laxmi Prasad Thapa
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, 100 Kalsanri, Tangjeonmyun, Asansi, Chungnam 336 708, Republic of Korea
    Appl Microbiol Biotechnol 76:1357-64. 2007
    ..It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid ..
  80. pmc Structure of Deinococcus radiodurans tunicamycin-resistance protein (TmrD), a phosphotransferase
    Ulrike Kapp
    European Synchrotron Radiation Facility, 6 rue Jules Horowitz, BP 220, 38043 Grenoble, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:479-86. 2008
    ..strong structural similarity to the family of nucleoside monophosphate kinases and to the chloramphenicol phosphotransferase of Streptomyces venezuelae, suggesting that the mode of action is possibly by phosphorylation of tunicamycin.
  81. pmc Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set
    Je Won Park
    Division of Nano Sciences, Ewha Womans University, 11 1 Daehyung dong, Seodaemun gu, Seoul 120 750, Republic of Korea
    Proc Natl Acad Sci U S A 105:8399-404. 2008
    ..related aminoglycoside-producing strains were introduced into a nonaminoglycoside producing strain of Streptomyces venezuelae. Heterologous expression of different combinations of putative 2-DOS biosynthetic genes revealed that a ..
  82. pmc The fused TrpEG from Streptomyces venezuelae is an anthranilate synthase, not a 2-amino-2-deoxyisochorismate [corrected] (ADIC) synthase
    Meseret Ashenafi
    Department of Biochemistry and Molecular Biology, College of Medicine, Howard University, Washington, DC 20059, USA
    Ethn Dis 18:S2-9-13. 2008
    The chloramphenicol producer Streptomyces venezuelae contains an enzyme, SvTrpEG, that has a high degree of amino acid sequence similarity to the phenazine biosynthetic enzyme PhzE of certain species of Pseudomonas...
  83. doi Heterologous production of epothilones B and D in Streptomyces venezuelae
    Sung Ryeol Park
    Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120 750, Republic of Korea
    Appl Microbiol Biotechnol 81:109-17. 2008
    ..The entire epothilone biosynthetic gene cluster was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin polyketide synthase gene cluster...
  84. pmc The methymycin/pikromycin pathway: a model for metabolic diversity in natural product biosynthesis
    Jeffrey D Kittendorf
    University of Michigan Life Sciences Institute, Department of Medicinal Chemistry, 210 Washtenaw Avenue, Ann Arbor, MI 48109 2216, USA
    Bioorg Med Chem 17:2137-46. 2009
    ..substrate flexibility, combining to produce six structurally diverse macrolide antibiotics in Streptomyces venezuelae. Thus, it is appealing to consider that the pikromycin biosynthetic enzymes could be leveraged for high-..
  85. pmc Antimicrobial activities of jadomycin B and structurally related analogues
    David L Jakeman
    College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
    Antimicrob Agents Chemother 53:1245-7. 2009
    ..We have isolated a series of structurally related polyketide-derived natural products from Streptomyces venezuelae ISP5230...
  86. pmc Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae
    Shengying Li
    Life Sciences Institute, Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 284:5723-30. 2009
    The cytochrome P-450 PikC from Streptomyces venezuelae exhibits significant substrate tolerance and performs multiple hydroxylation reactions on structurally variant macrolides bearing the deoxyamino sugar desosamine...
  87. ncbi Identification and functional characterization of an afsR homolog regulatory gene from Streptomyces venezuelae ATCC 15439
    Sushila Maharjan
    Department of Pharmaceutical Engineering, SunMoon University, Asansi 336 708, Korea
    J Microbiol Biotechnol 19:121-7. 2009
    Sequencing analysis of a 5-kb DNA fragment from Streptomyces venezuelae ATCC 15439 revealed the presence of one 3.1-kb open reading frame (ORF), designated afsRsv...
  88. doi Combinatorial biosynthesis of 5-O-desosaminyl erythronolide A as a potent precursor of ketolide antibiotics
    Devi B Basnet
    Division of Nano Sciences, Ewha Womans University, Seoul 120 750, Republic of Korea
    J Biotechnol 135:92-6. 2008
    ..gene pikC, which encodes the substrate-flexible hydroxylase from the pikromycin biosynthetic pathway of Streptomyces venezuelae, in the eryBV deletion mutant strain of Sac. erythraea led to 5-O-desosaminyl erythronolide A production.
  89. doi Metabolic engineering of noviose: heterologous expression of novWUS and generation of a new hybrid antibiotic, noviosylated 10-deoxymethynolide/narbonolide, from Streptomyces venezuelae YJ003-OTBP1
    Binod Babu Pageni
    Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100, Kalsan ri, Tangjeong myeon, Asan Si, Chungnam 336 708, Republic of Korea
    Biotechnol Lett 30:1609-15. 2008
    ..This established the function of novWUS and, at the same time, it also proved that the noviosyl derivative of 10-deoxymethynolide(2)/narbonolide(4) obtained from S. venezuelae YJ003-OTBP1 is a novel hybrid antibiotic...
  90. ncbi Identification by gene deletion analysis of barS2, a gene involved in the biosynthesis of gamma-butyrolactone autoregulator in Streptomyces virginiae
    Yong Jik Lee
    International Center for Biotechnology, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Arch Microbiol 189:367-74. 2008
    ..The deduced product of barS2 possessed moderate similarity to a putative dehydrogenase of Streptomyces venezuelae, encoded by jadW2 located in similar gene arrangement to that in the regulatory island of S. virginiae...
  91. doi Total synthesis of 10-deoxymethynolide and narbonolide
    Richeng Xuan
    Department of Chemistry, Chungbuk National University, Cheongju, Chungbuk 361 763, Korea
    J Org Chem 73:1456-61. 2008
    ..These lactones are produced by pikromycin polyketide synthase from Streptomyces venezuelae. Polyketide lactones, 10-deoxymethynolide and narbonolide, which contain 12- and 14-membered rings, ..
  92. pmc Enhanced heterologous production of desosaminyl macrolides and their hydroxylated derivatives by overexpression of the pikD regulatory gene in Streptomyces venezuelae
    Won Seok Jung
    Division of Nano Sciences, Ewha Womans University, Seoul 120 750, South Korea
    Appl Environ Microbiol 74:1972-9. 2008
    To elevate the production level of heterologous polyketide in Streptomyces venezuelae, an additional copy of the positive regulatory gene pikD was introduced into the pikromycin (Pik) polyketide synthase (PKS) deletion mutant of S...
  93. doi Biosynthesis of spectinomycin: heterologous production of spectinomycin and spectinamine in an aminoglycoside-deficient host, Streptomyces venezuelae YJ003
    L P Thapa
    Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Chungnam, Republic of Korea
    J Appl Microbiol 105:300-8. 2008
    To obtain spectinomycin and spectinamine by heterologous expression into the biosynthetic deoxysugar (desosamine) gene-deleted host Streptomyces venezuelae YJ003.
  94. doi Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin
    Sushila Maharjan
    Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, 100, Kalsan ri, Tangjeong myeon, Asan Si, Chungnam 336 708, Republic of Korea
    Biotechnol Lett 30:1621-6. 2008
    ..The elevated transcripts of the pikD and ketosynthase genes were consistent with the enhanced production of pikromycin...
  95. ncbi Further biological and molecular characterization of actinophage VWB
    J Anne
    Laboratory of Microbiology, University of Leuven, Belgium
    J Gen Microbiol 136:1365-72. 1990
    ..f.u. Although phage VWB could infect only Streptomyces venezuelae ETH 14630 (ATCC 40755), of six different S...
  96. ncbi Management of anemic effects of chloramphenicol using amino acids on albino rats infected with Salmonella typhi
    K C Chilaka
    Department of Pharmacology and Therapeutics, College of Health Sciences, Nnamdi Azikwe University, Nnewi Campus, Anambra State, Nigeria
    Am J Ther 13:118-20. 2006
    Chloramphenicol is an antibiotic originally extracted from the fungus Streptomyces venezuelae, but it is now synthesized. It is a drug of choice for many of its toxicity in the blood-forming organs...
  97. ncbi Expression of biologically active human tumor necrosis factor beta by Streptomyces lividans
    Bin Hong
    Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    Yi Chuan Xue Bao 30:209-14. 2003
    ..translation and secretion signals of subtilisin inhibitor VSI which is naturally produced by Streptomyces venezuelae CBS762.70...
  98. ncbi Conjugative DNA transfer in Streptomyces: SpdB2 involved in the intramycelial spreading of plasmid pSVH1 is an oligomeric integral membrane protein that binds to dsDNA
    Yvonne Tiffert
    Mikrobiologie Biotechnologie, Mikrobiologisches Institut, Fakultat fur Biologie, Eberhard Karls Universitat Tubingen, Auf der Morgenstelle 28, 72076 Tubingen, Germany
    Microbiology 153:2976-83. 2007
    ..spdB2 of the conjugative pSVH1 plasmid of Streptomyces venezuelae was heterologously expressed in Escherichia coli and Streptomyces lividans, with a C-terminal His-tag-..

Research Grants51

  1. BIOSYNTHESIS OF AROMATIC POLYKETIDES
    CHARLES SIH; Fiscal Year: 2001
    ..For jadomycin biosynthesis in Streptomyces venezuelae: (a) Express and purify the Jad-Orf4 polyketide cyclase...
  2. BIOCHEMISTRY AND MOLECULAR GENETICS OF MITOMYCIN BIOSYNT
    David Sherman; Fiscal Year: 2003
    ..Overall, this work will provide an important theoretical and experimental base for future combinatorial biology-based production of novel AHBA-derived natural products using molecular genetic technology. ..
  3. Genomic Analysis of Antibiotic Biosynthesis
    David Sherman; Fiscal Year: 2005
    ..coelicolor genome. ..
  4. Molecular Analysis of Modular Polyketide Synthases
    David Sherman; Fiscal Year: 2007
    ....
  5. Structure and Engineering of Natural Product Cytochrome P450 Enzymes
    David Sherman; Fiscal Year: 2007
    ..Specific Aim 2. Explore the role of macrolide sugar-mediated anchoring on substrate binding and catalytic activity of PikC. Specific Aim 3. Engineering of novel macrolide hydroxylases. ..
  6. Structure and Engineering of Natural Product Cytochrome P450 Enzymes
    David H Sherman; Fiscal Year: 2010
    ..Specific Aim 2. Explore the role of macrolide sugar-mediated anchoring on substrate binding and catalytic activity of PikC. Specific Aim 3. Engineering of novel macrolide hydroxylases. ..
  7. Biosynthetic Analysis of Marine Cyanobacterial Pathways
    David H Sherman; Fiscal Year: 2010
    ....
  8. Biosynthetic Analysis of Marine Cyanobacterial Pathways
    David Sherman; Fiscal Year: 2007
    ....
  9. Molecular Analysis of Modular Polyketide Synthases
    David Sherman; Fiscal Year: 2009
    ..abstract_text> ..
  10. COMBINATORIAL BIOSYNTHESIS AND THE PIKROMYCIN PATHWAY
    David Sherman; Fiscal Year: 2002
    ..key tailoring enzymes, and overall pathway regulation in the pikromycin (pik) biosynthetic pathway from Streptomyces venezuelae. The pikromycin system includes a set of 18 genes residing on 60 kb of DNA with a locus containing two ..
  11. MOLECULAR GENETIC STUDIES OF METHYMYCIN BIOSYNTHESIS
    David Sherman; Fiscal Year: 1993
    ..to establish an effective experimental model, we will investigate the methymycin (met) type I PKS of Streptomyces venezuelae. Although we expect the molecular genetic details will have important comparative value, this work will ..
  12. METABOLIC ENGINEERING OF CEPHAMYCIN C BIOSYNTHESIS
    David Sherman; Fiscal Year: 2000
    ..Please visit: http://www.cems.umn.edu/~hu-grp/gfp/nih.html) ..