Rhodococcus jostii RHA1


Alias: Rhodococcus sp. RHA1, Rhodococcus jostii str. RHA1, Rhodococcus jostii strain RHA1, Rhodococcus sp. (strain RHA1)

Top Publications

  1. Villalba M, Alvarez H. Identification of a novel ATP-binding cassette transporter involved in long-chain fatty acid import and its role in triacylglycerol accumulation in Rhodococcus jostii RHA1. Microbiology. 2014;160:1523-32 pubmed publisher
    ..TAG metabolism has yet been characterized in these bacteria, we used the available genomic information of Rhodococcus jostii RHA1 to perform a broad survey of genes coding for putative lipid transporter proteins in this oleaginous ..
  2. Ros oniec K, Wilbrink M, Capyk J, Mohn W, Ostendorf M, van der Geize R, et al. Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1. Mol Microbiol. 2009;74:1031-43 pubmed publisher
    The cyp125 gene of Rhodococcus jostii RHA1 was previously found to be highly upregulated during growth on cholesterol and the orthologue in Mycobacterium tuberculosis (rv3545c) has been implicated in pathogenesis...
  3. Nagy I, Tamura T, Vanderleyden J, Baumeister W, De Mot R. The 20S proteasome of Streptomyces coelicolor. J Bacteriol. 1998;180:5448-53 pubmed
    ..and showed that the beta subunit is encoded with a 53-amino-acid propeptide which is removed during proteasome assembly. The upstream DNA region contains the conserved orf7 and an AAA ATPase gene (arc). ..
  4. Indest K, Eberly J, Hancock D, Jung C, Carr M, Blakeney G. Rhodococcus jostii RHA1 TadA-homolog deletion mutants accumulate less polyhydroxyalkanoates (PHAs) than the parental strain. J Gen Appl Microbiol. 2016;62:213-6 pubmed publisher
  5. Mayaux J, Cerebelaud E, Soubrier F, Faucher D, Petre D. Purification, cloning, and primary structure of an enantiomer-selective amidase from Brevibacterium sp. strain R312: structural evidence for genetic coupling with nitrile hydratase. J Bacteriol. 1990;172:6764-73 pubmed
    ..Large amounts of an active recombinant enzyme could be produced in Escherichia coli at 30 degrees C under the control of an E. coli promoter and ribosome-binding site. ..
  6. Nguyen Q, de Gonzalo G, Binda C, Rioz Martínez A, Mattevi A, Fraaije M. Biocatalytic Properties and Structural Analysis of Eugenol Oxidase from Rhodococcus jostii RHA1: A Versatile Oxidative Biocatalyst. Chembiochem. 2016;17:1359-66 pubmed publisher
    Eugenol oxidase (EUGO) from Rhodococcus jostii RHA1 had previously been shown to convert only a limited set of phenolic compounds...
  7. Choi K, Kim D, Koh S, So J, Kim J, Kim E. Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene. J Microbiol. 2004;42:160-2 pubmed
  8. Vereecke D, Cornelis K, Temmerman W, Jaziri M, Van Montagu M, Holsters M, et al. Chromosomal locus that affects pathogenicity of Rhodococcus fascians. J Bacteriol. 2002;184:1112-20 pubmed
    ..Hence, such interaction could be referred to as metabolic habitat modification...
  9. Hart S, Kirby R, Woods D. Structure of a Rhodococcus gene encoding pigment production in Escherichia coli. J Gen Microbiol. 1990;136:1357-63 pubmed
    ..This small pigment gene may become useful for the development of a new generation of chromogenic cloning vectors which do not require expensive substrates for the detection of gene expression. ..

More Information


  1. O Mahony R, Doran J, Coffey L, Cahill O, Black G, O Reilly C. Characterisation of the nitrile hydratase gene clusters of Rhodococcus erythropolis strains AJ270 and AJ300 and Microbacterium sp. AJ115 indicates horizontal gene transfer and reveals an insertion of IS1166. Antonie Van Leeuwenhoek. 2005;87:221-32 pubmed
    ..The nitrile hydratase/amidase enzymes of strain AJ270 are inducible with acetonitrile or acetamide. Interestingly although a number of Fe-type nitrile hydratases have been shown to be photosensitive, the enzyme from strain AJ270 is not...
  2. Vereecke D, Cornelis K, Temmerman W, Holsters M, Goethals K. Versatile persistence pathways for pathogens of animals and plants. Trends Microbiol. 2002;10:485-8 pubmed
    ..Hence, the ability to switch diet inside the host appears to be governed by 'persistence' enzymes that are conserved between pathogens of animals and plants...
  3. Tanaka Y, Yoshikawa O, Maruhashi K, Kurane R. The cbs mutant strain of Rhodococcus erythropolis KA2-5-1 expresses high levels of Dsz enzymes in the presence of sulfate. Arch Microbiol. 2002;178:351-7 pubmed
    ..The cbs mutants also expressed high levels of Dsz enzymes when methionine was used as the sole source of sulfur. ..
  4. van der Geize R, Yam K, Heuser T, Wilbrink M, Hara H, Anderton M, et al. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Proc Natl Acad Sci U S A. 2007;104:1947-52 pubmed publisher
    ..The results suggest that cholesterol metabolism is central to M. tuberculosis's unusual ability to survive in macrophages and provide insights into potential targets for novel therapeutics...
  5. Soong C, Ogawa J, Shimizu S. Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase. Biochem Biophys Res Commun. 2001;286:222-6 pubmed publisher
    ..The existence of a consecutive enzyme named ureidomalonase, which hydrolyzes ureidomalonic acid to urea and malonic acid, was also discovered during the purification of barbiturase...
  6. Nagy I, De Mot R. Sequence analysis of the oxidase/reductase genes upstream of the Rhodococcus erythropolis aldehyde dehydrogenase gene thcA reveals a gene organisation different from Mycobacterium tuberculosis. DNA Seq. 1999;10:61-6 pubmed
    ..In Mycobacterium tuberculosis H37Rv, most of these ORFs have homologs that are also clustered in the genome, but some striking differences in gene organization were observed between Rhodococcus and Mycobacterium...
  7. Wang Y, Shimodaira J, Miyasaka T, Morimoto S, Oomori T, Ogawa N, et al. Detection of bphAa gene expression of Rhodococcus sp. strain RHA1 in soil using a new method of RNA preparation from soil. Biosci Biotechnol Biochem. 2008;72:694-701 pubmed publisher
  8. van Oosterwijk N, Knol J, Dijkhuizen L, van der Geize R, Dijkstra B. Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of 3-ketosteroid ?(4)-(5?)-dehydrogenase from Rhodococcus jostii RHA1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:1269-73 pubmed publisher
    ..Seeding greatly improved the number of crystals obtained. The crystals belonged to space group C222(1), with unit-cell parameters a = 99.2, b = 114.3, c = 110.2 Å. Data were collected to a resolution of 1.6 Å. ..
  9. Kalkus J, Menne R, Reh M, Schlegel H. The terminal structures of linear plasmids from Rhodococcus opacus. Microbiology. 1998;144 ( Pt 5):1271-9 pubmed
    ..The results support the assumption that long TIRs are not absolutely necessary for replication and maintenance of linear plasmids. ..
  10. Casabon I, Crowe A, Liu J, Eltis L. FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. Mol Microbiol. 2013;87:269-83 pubmed publisher
    The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection...
  11. Tsujimura M, Dohmae N, Odaka M, Chijimatsu M, Takio K, Yohda M, et al. Structure of the photoreactive iron center of the nitrile hydratase from Rhodococcus sp. N-771. Evidence of a novel post-translational modification in the cysteine ligand. J Biol Chem. 1997;272:29454-9 pubmed
    ..These results indicate that the NHase from Rhodococcus sp. N-771 has a novel non-heme iron enzyme containing a cysteine-sulfinic acid in the iron center. Possible ligand residues of the iron center are discussed. ..
  12. Huang W, Jia J, Cummings J, Nelson M, Schneider G, Lindqvist Y. Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold. Structure. 1997;5:691-9 pubmed
    ..The low-spin electronic configuration presumably results in part from two Fe-amide nitrogen bonds. The structure is consistent with the metal ion having a role as a Lewis acid in the catalytic reaction...
  13. Hernández M, Comba S, Arabolaza A, Gramajo H, Alvarez H. Overexpression of a phosphatidic acid phosphatase type 2 leads to an increase in triacylglycerol production in oleaginous Rhodococcus strains. Appl Microbiol Biotechnol. 2015;99:2191-207 pubmed publisher
    ..In this work, we identified and characterized a putative PAP type 2 (PAP2) encoded by the ro00075 gene in Rhodococcus jostii RHA1. Heterologous expression of ro00075 in Escherichia coli resulted in a fourfold increase in PAP activity ..
  14. De Mot R, Nagy I, Schoofs G, Vanderleyden J. Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from Rhodococcus sp. NI86/21. Gene. 1994;143:91-3 pubmed
    ..The protein deduced from a third Rhodococcus gene aligns well with CobL of P. denitrificans and with the protein pair CbiE/CbiT of S. typhimurium, which are involved in corrin methylation and decarboxylation. ..
  15. Wolf S, Nagy I, Lupas A, Pfeifer G, Cejka Z, M ller S, et al. Characterization of ARC, a divergent member of the AAA ATPase family from Rhodococcus erythropolis. J Mol Biol. 1998;277:13-25 pubmed publisher
    ..Two-dimensional crystals grown on lipid monolayers yielded images of the ATPase molecules in "end-on" orientation at 1.9 nm resolution...
  16. Jang J, Kim D, Bae H, Choi K, Chae J, Zylstra G, et al. Isolation and characterization of a rhodococcus species strain able to grow on ortho- and para-xylene. J Microbiol. 2005;43:325-30 pubmed
    ..PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJYJ1. ..
  17. Furusawa Y, Nagarajan V, Tanokura M, Masai E, Fukuda M, Senda T. Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1. J Mol Biol. 2004;342:1041-52 pubmed publisher
    ..The cooperative actions of residues in the three groups seem to determine the substrate specificity of the enzyme...
  18. Dong L, Nakashima N, Tamura N, Tamura T. Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: application for recombinant protein expression in Rhodococcus. FEMS Microbiol Lett. 2004;237:35-40 pubmed publisher
    ..The tipA gene is presumed to be transcribed into a leaderless mRNA. We applied the tipA promoter successfully for recombinant protein expression in R. erythropolis cells...
  19. Denger K, Ruff J, Schleheck D, Cook A. Rhodococcus opacus expresses the xsc gene to utilize taurine as a carbon source or as a nitrogen source but not as a sulfur source. Microbiology. 2004;150:1859-67 pubmed
    ..A putative tauD gene (with three other candidates) was detected in strain ISO-5. Regulation of the different forms of metabolism of taurine remains to be elucidated. ..
  20. Wall D, Duffy P, Dupont C, Prescott J, Meijer W. Isocitrate lyase activity is required for virulence of the intracellular pathogen Rhodococcus equi. Infect Immun. 2005;73:6736-41 pubmed publisher
    ..In conclusion, the aceA gene was shown to be essential for virulence of R. equi, suggesting that membrane lipids may be an important source of carbon for phagocytosed R. equi...
  21. Takeda H, Yamada A, Miyauchi K, Masai E, Fukuda M. Characterization of transcriptional regulatory genes for biphenyl degradation in Rhodococcus sp. strain RHA1. J Bacteriol. 2004;186:2134-46 pubmed
    ..Growth was restored by introduction of an intact bphS gene into SDR1. These results indicate that at least bphS is indispensably responsible for the growth of RHA1 on biphenyl...
  22. Sakai M, Masai E, Asami H, Sugiyama K, Kimbara K, Fukuda M. Diversity of 2,3-dihydroxybiphenyl dioxygenase genes in a strong PCB degrader, Rhodococcus sp. strain RHA1. J Biosci Bioeng. 2002;93:421-7 pubmed
    ..In contrast, the bphC2, bphC3, and bphC4 genes may not be involved in biphenyl and PCB degradation...
  23. Witt S, Kwon Y, Sharon M, Felderer K, Beuttler M, Robinson C, et al. Proteasome assembly triggers a switch required for active-site maturation. Structure. 2006;14:1179-88 pubmed
    ..These data support the proposed assembly-dependent activation model in which the S2-S3 loop acts as an activation switch. ..
  24. Sakai M, Miyauchi K, Kato N, Masai E, Fukuda M. 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 2003;69:427-33 pubmed
  25. De Mot R, Nagy I, Schoofs G, Vanderleyden J. Sequence of Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease. Can J Microbiol. 1994;40:403-7 pubmed
    ..A truncated open reading frame downstream of eutC contained an N-terminal motif characteristic of membrane-anchored lipoproteins. ..
  26. Kelly B, Wall D, Boland C, Meijer W. Isocitrate lyase of the facultative intracellular pathogen Rhodococcus equi. Microbiology. 2002;148:793-8 pubmed publisher
    ..It is proposed that a stable hairpin structure with a free energy (DeltaG) of -28.5 kcal x mol(-1) and located in the 90 bp aceA-fadB intergenic region is involved in stabilizing the aceA transcript...
  27. Otani H, Stogios P, Xu X, Nocek B, Li S, Savchenko A, et al. The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism. Nucleic Acids Res. 2016;44:595-607 pubmed publisher
    ..repressor, regulates the cou genes, encoding p-hydroxycinnamate catabolism in the soil bacterium Rhodococcus jostii RHA1. The CouR dimer bound two molecules of the catabolite p-coumaroyl-CoA (Kd = 11 ± 1 μM)...
  28. Boland C, Meijer W. The iron dependent regulatory protein IdeR (DtxR) of Rhodococcus equi. FEMS Microbiol Lett. 2000;191:1-5 pubmed
    ..diphtheriae in an iron dependent manner. An open reading frame specifying a 283-amino acid polypeptide similar to galE encoding UDP-galactose 4-epimerase was present downstream of the ideR gene. ..
  29. Masai E, Sugiyama K, Iwashita N, Shimizu S, Hauschild J, Hatta T, et al. The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1. Gene. 1997;187:141-9 pubmed
    ..Southern blot analysis indicated that the meta-cleavage pathway for biphenyl/PCB catabolism in RHA1 is directed by the 390 kb plasmid borne bphDEF genes located separately from bphACB gene cluster on the 1100 kb plasmid...
  30. Nagy I, Geert S, Jos V, De Mot R. Further sequence analysis of the DNA regions with the Rhodococcus 20S proteasome structural genes reveals extensive homology with Mycobacterium leprae. DNA Seq. 1997;7:225-8 pubmed
    ..These downstream ORFs also show a significant level of sequence homology with the ORF6(1) and ORF6(2) which are located upstream of the proteasome structural genes in the respective clusters. ..
  31. Masai E, Yamada A, Healy J, Hatta T, Kimbara K, Fukuda M, et al. Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 1995;61:2079-85 pubmed
    ..coli in an in vitro transcription-translation system. Insertion mutations of bphA1 and bphC of Rhodococcus sp. strain RHA1 were constructed by gene replacement with cloned gene fragments.(ABSTRACT TRUNCATED AT 250 WORDS)..
  32. Ikehata O, Nishiyama M, Horinouchi S, Beppu T. Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli. Eur J Biochem. 1989;181:563-70 pubmed
    ..The activity of the enzyme was restored, to some extent, by solubilization of the proteins with 8 M urea and subsequent dialysis for refolding at pH 10 in the presence of Fe2+ and pyrroloquinoline quinone. ..
  33. Faust A, Niefind K, Hummel W, Schomburg D. The structure of a bacterial L-amino acid oxidase from Rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation. J Mol Biol. 2007;367:234-48 pubmed publisher
    ..These results are of general relevance for the mechanism of flavoproteins and lead to the proposal of a common dehydrogenation step in the mechanism for l and d-amino acid oxidases...
  34. Takeo M, Yasukawa T, Abe Y, Niihara S, Maeda Y, Negoro S. Cloning and characterization of a 4-nitrophenol hydroxylase gene cluster from Rhodococcus sp. PN1. J Biosci Bioeng. 2003;95:139-45 pubmed
    ..J. Bacteriol., 182, 627-636, 2000). ..
  35. Okamoto S, Eltis L. Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1. Mol Microbiol. 2007;65:828-38 pubmed publisher
    ..We propose that this acetonitrile hydratase (ANHase) represents the first member of a previously unknown class of NHases...
  36. Nagy I, Verheijen S, De Schrijver A, Van Damme J, Proost P, Schoofs G, et al. Characterization of the Rhodococcus sp. NI86/21 gene encoding alcohol: N,N'-dimethyl-4-nitrosoaniline oxidoreductase inducible by atrazine and thiocarbamate herbicides. Arch Microbiol. 1995;163:439-46 pubmed
    ..N-Terminal sequence analysis of purified MNO from Rhodococcus sp. NI86/21 confirmed the identity with ThcE. When overproduced in Escherichia coli, ThcE was insoluble and no MNO activity was detected...
  37. Somalinga V, Mohn W. Rhodococcus jostii porin A (RjpA) functions in cholate uptake. Appl Environ Microbiol. 2013;79:6191-3 pubmed publisher
    ..jostii on cholate but not on cholesterol. Eventual growth on cholate involved increased expression of other porins, namely, RjpB, RjpC, and RjpD. Porins appear essential for the uptake of bile acids by mycolic acid bacteria. ..
  38. Vereecke D, Villarroel R, Van Montagu M, Desomer J. Cloning and sequence analysis of the gene encoding isocitrate lyase from Rhodococcus fascians. Gene. 1994;145:109-14 pubmed
    ..Comparison of the prokaryotic and eukaryotic Icl confirms the earlier proposal of Matsuoka and McFadden [J. Bacteriol. 143 (1988) 4528-4536] that the enzyme has enlarged during evolution. ..
  39. Larkin M, Allen C, Kulakov L, Lipscomb D. Purification and characterization of a novel naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038. J Bacteriol. 1999;181:6200-4 pubmed
    ..The deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in Pseudomonas putida NCIB 9816-4, for which the tertiary structure has been reported. ..
  40. Nagy I, Schoofs G, Compernolle F, Proost P, Vanderleyden J, De Mot R. Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase. J Bacteriol. 1995;177:676-87 pubmed
    ..strain NI86/21. In addition to the major metabolite N-depropyl EPTC, another degradation product was identified, EPTC-sulfoxide...
  41. Komeda H, Kobayashi M, Shimizu S. Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc Natl Acad Sci U S A. 1996;93:4267-72 pubmed
  42. Eulberg D, Golovleva L, Schlömann M. Characterization of catechol catabolic genes from Rhodococcus erythropolis 1CP. J Bacteriol. 1997;179:370-81 pubmed
  43. Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, et al. Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nat Struct Biol. 1998;5:347-51 pubmed
    ..This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins...
  44. Powell J, Archer J. Molecular characterisation of a Rhodococcus ohp operon. Antonie Van Leeuwenhoek. 1998;74:175-88 pubmed
  45. Nojiri M, Yohda M, Odaka M, Matsushita Y, Tsujimura M, Yoshida T, et al. Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine. J Biochem. 1999;125:696-704 pubmed
    ..Although another modification of alphaCys114 could not be identified because of the instability under acidic conditions, the recombinant NHase could be reversibly inactivated by nitric oxide...
  46. Khamis A, Raoult D, La Scola B. rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol. 2004;42:3925-31 pubmed publisher
    ..The sequence of this fragment (434 to 452 bp) allows accurate species identification and may be used in the future for routine sequence-based identification of Corynebacterium species...
  47. Soong C, Ogawa J, Sakuradani E, Shimizu S. Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism. J Biol Chem. 2002;277:7051-8 pubmed publisher
    ..The full-length gene encoding the barbiturase (bar) was cloned and overexpressed in Escherichia coli. The kinetic parameters and physicochemical properties of the cloned enzyme were apparently similar to those of the wild-type...
  48. Xie S, Kato Y, Komeda H, Yoshida S, Asano Y. A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4. Biochemistry. 2003;42:12056-66 pubmed
    ..e., NHase and amidase, and their regulatory and activator proteins. We report here the existence of an aldoxime dehydratase genetically linked with NHase and amidase, and responsible for the metabolism of alkylaldoxime in R. globerulus. ..
  49. Priefert H, O Brien X, Lessard P, Dexter A, Choi E, Tomic S, et al. Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24. Appl Microbiol Biotechnol. 2004;65:168-76 pubmed publisher
    ..The results indicate the role of the tid operon in the bioconversion of indene to 1-indenol and cis-(1S,2R)-indandiol by Rhodococcus sp. I24...
  50. Tamura T, Nagy I, Lupas A, Lottspeich F, Cejka Z, Schoofs G, et al. The first characterization of a eubacterial proteasome: the 20S complex of Rhodococcus. Curr Biol. 1995;5:766-74 pubmed
    ..The recent description of the first eubacterial ubiquitin, and our discovery of a eubacterial proteasome show that the ubiquitin pathway of protein degradation is ancestral and common to all forms of life...
  51. Iwasaki T, Takeda H, Miyauchi K, Yamada T, Masai E, Fukuda M. Characterization of two biphenyl dioxygenases for biphenyl/PCB degradation in A PCB degrader, Rhodococcus sp. strain RHA1. Biosci Biotechnol Biochem. 2007;71:993-1002 pubmed publisher
    ..The wide substrate preference of EtbA/EbdA dioxygenase suggested that it plays a more important role in polychlorinated biphenyl (PCB) degradation than does BphA dioxygenase...
  52. Kitagawa W, Suzuki A, Hoaki T, Masai E, Fukuda M. Multiplicity of aromatic ring hydroxylation dioxygenase genes in a strong PCB degrader, Rhodococcus sp. strain RHA1 demonstrated by denaturing gradient gel electrophoresis. Biosci Biotechnol Biochem. 2001;65:1907-11 pubmed
    ..The gene of band E was not induced by any of these substrates. Thus the combination of DGGE and Southern hybridization enable us to address the multiplicity of the ring hydroxylation dioxygenase genes and to isolate some of them...
  53. Kitagawa W, Miyauchi K, Masai E, Fukuda M. Cloning and characterization of benzoate catabolic genes in the gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. J Bacteriol. 2001;183:6598-606 pubmed
    ..In contrast, two rhodococcal strains that cannot transform benzoate were found not to have RHA1 benABC homologs, suggesting that many Rhodococcus strains contain benzoate catabolic genes similar to RHA1 benABC. ..
  54. Nagarajan V, Sakurai N, Kubota M, Nonaka T, Nagumo H, Takeda H, et al. Crystallization of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1. Protein Pept Lett. 2003;10:412-7 pubmed
    ..The crystals belong to the tetragonal system in the space group P4(1)2(1)2 or P4(3)2(1)2 and diffract to better than 2.2A resolution. ..
  55. Swain K, Casabon I, Eltis L, Mohn W. Two transporters essential for reassimilation of novel cholate metabolites by Rhodococcus jostii RHA1. J Bacteriol. 2012;194:6720-7 pubmed publisher
    ..We investigated two transporters associated with cholate catabolism in Rhodococcus jostii RHA1. Reverse transcriptase quantitative-PCR indicated that an ATP-binding cassette (ABC) transporter and a ..
  56. Yamada A, Kishi H, Sugiyama K, Hatta T, Nakamura K, Masai E, et al. Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 1998;64:2006-12 pubmed
    ..Strain RCD1, an RHA1 mutant strain lacking both the bphD gene and the etbD2 gene, grew well on ethylbenzene. This result suggested that the etbD1 gene product is involved in the meta-cleavage metabolic pathway of ethylbenzene. ..
  57. Endo T, Watanabe I. Nitrile hydratase of Rhodococcus sp. N-774. Purification and amino acid sequences. FEBS Lett. 1989;243:61-4 pubmed
    ..The activity of the purified enzyme did not decrease during incubation in the dark, whereas it gradually decreased in intact cells. ..
  58. Kwon Y, Nagy I, Adams P, Baumeister W, Jap B. Crystal structures of the Rhodococcus proteasome with and without its pro-peptides: implications for the role of the pro-peptide in proteasome assembly. J Mol Biol. 2004;335:233-45 pubmed
    ..This appears to be a fail-safe mechanism ensuring that the active sites on the beta-subunits are activated only after proteasome assembly is complete. ..
  59. Hauschild J, Masai E, Sugiyama K, Hatta T, Kimbara K, Fukuda M, et al. Identification of an alternative 2,3-dihydroxybiphenyl 1,2-dioxygenase in Rhodococcus sp. strain RHA1 and cloning of the gene. Appl Environ Microbiol. 1996;62:2940-6 pubmed
    ..The possible implication of coexpressed BphC extradiol dioxygenases in the strong polychlorinated-biphenyl degradation activity of RHA1 was suggested. ..