Mycobacterium smegmatis MC2


Alias: Mycobacterium smegmatis str. MC2 155, Mycobacterium smegmatis str. MC2, Mycobacterium smegmatis 'MC2 155', Mycobacterium smegmatis MC2 155, Mycobacterium smegmatis strain MC2 155

Top Publications

  1. Stadthagen G, Sambou T, Guerin M, Barilone N, Boudou F, Kordulakova J, et al. Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. J Biol Chem. 2007;282:27270-6 pubmed
    ..Results thus suggest that Rv3032 encodes the alpha-(1-->4)-glucosyltransferase responsible for the elongation of MGLP, whereas MSMEG2349 encodes the O-methyltransferase required for the 6-O-methylation of these compounds. ..
  2. Morita Y, Sena C, Waller R, Kurokawa K, Sernee M, Nakatani F, et al. PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria. J Biol Chem. 2006;281:25143-55 pubmed
    ..Taken together, PimE represents the first PPM-dependent mannosyl-transferase shown to be involved in PIM biosynthesis, where it mediates the fifth mannose transfer. ..
  3. Li W, Xin Y, McNeil M, Ma Y. rmlB and rmlC genes are essential for growth of mycobacteria. Biochem Biophys Res Commun. 2006;342:170-8 pubmed
    ..These results demonstrate that rmlB and rmlC genes are essential for mycobacterial growth, therefore, RmlB and RmlC are essential targets to develop new anti-tuberculosis drugs. ..
  4. Stephan J, Bender J, Wolschendorf F, Hoffmann C, Roth E, Mailänder C, et al. The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. Mol Microbiol. 2005;58:714-30 pubmed
    ..These results show that porin-mediated influx of nutrients is a major determinant of the growth rate of M. smegmatis. ..
  5. Sondén B, Kocíncová D, Deshayes C, Euphrasie D, Rhayat L, Laval F, et al. Gap, a mycobacterial specific integral membrane protein, is required for glycolipid transport to the cell surface. Mol Microbiol. 2005;58:426-40 pubmed
    ..This Gap family represents a new paradigm for the transport of small molecules across the mycobacterial envelope, a critical determinant of mycobacterial virulence. ..
  6. Hett E, Chao M, Deng L, Rubin E. A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor. PLoS Pathog. 2008;4:e1000001 pubmed publisher
    ..These data reveal the unusual essentiality of a peptidoglycan hydrolase and suggest a novel protein-protein interaction as one way of regulating its activity. ..
  7. O Hare H, Duran R, Cerveñansky C, Bellinzoni M, Wehenkel A, Pritsch O, et al. Regulation of glutamate metabolism by protein kinases in mycobacteria. Mol Microbiol. 2008;70:1408-23 pubmed publisher
    ..Previous reports of a defect in glutamate metabolism caused by pknG deletion may thus be explained by the effect of unphosphorylated GarA on these two enzyme activities, which may also contribute to the attenuation of virulence. ..
  8. Siegrist M, Unnikrishnan M, McConnell M, Borowsky M, Cheng T, Siddiqi N, et al. Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition. Proc Natl Acad Sci U S A. 2009;106:18792-7 pubmed publisher
    ..Mycobacteria thus require a specialized secretion system for acquiring iron from siderophores. ..
  9. Kordulakova J, Gilleron M, Puzo G, Brennan P, Gicquel B, Mikusova K, et al. Identification of the required acyltransferase step in the biosynthesis of the phosphatidylinositol mannosides of mycobacterium species. J Biol Chem. 2003;278:36285-95 pubmed
    ..We also provide the first evidence that two distinct pathways lead to the formation of acylated PIM2 from PIM1 in mycobacteria. ..

More Information


  1. Datta S, Ganesh N, Chandra N, Muniyappa K, Vijayan M. Structural studies on MtRecA-nucleotide complexes: insights into DNA and nucleotide binding and the structural signature of NTP recognition. Proteins. 2003;50:474-85 pubmed
    ..Comparison with other NTP binding proteins reveals many commonalties in modes of binding by diverse members in the structural family, contributing to our understanding of the structural signature of NTP recognition. ..
  2. Stahl C, Kubetzko S, Kaps I, Seeber S, Engelhardt H, Niederweis M. MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis. Mol Microbiol. 2001;40:451-64 pubmed
  3. Kendall S, Withers M, Soffair C, Moreland N, Gurcha S, Sidders B, et al. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Mol Microbiol. 2007;65:684-99 pubmed
    ..tuberculosis. We conclude that this regulator, designated elsewhere as kstR, controls the expression of genes used for utilizing diverse lipids as energy sources, possibly imported through the mce4 system. ..
  4. Shi W, Zhang X, Jiang X, Yuan H, Lee J, Barry C, et al. Pyrazinamide inhibits trans-translation in Mycobacterium tuberculosis. Science. 2011;333:1630-2 pubmed publisher
    ..Trans-translation is essential for freeing scarce ribosomes in nonreplicating organisms, and its inhibition may explain the ability of PZA to eradicate persisting organisms. ..
  5. Schaeffer M, Khoo K, Besra G, Chatterjee D, Brennan P, Belisle J, et al. The pimB gene of Mycobacterium tuberculosis encodes a mannosyltransferase involved in lipoarabinomannan biosynthesis. J Biol Chem. 1999;274:31625-31 pubmed
    ..smegmatis overexpressing the pimB gene were used in cell-free assays to show that PimB catalyzes the formation of triacylphosphatidylinositol dimannoside from GDP-mannose and triacylphosphatidylinositol monomannoside. ..
  6. Datta S, Prabu M, Vaze M, Ganesh N, Chandra N, Muniyappa K, et al. Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation. Nucleic Acids Res. 2000;28:4964-73 pubmed
    ..The formation of a helical filament with a deep groove appears to be an inherent property of MtRecA. The histidine in loop L1 appears to be positioned appropriately for DNA interaction. ..
  7. Nambi S, Basu N, Visweswariah S. cAMP-regulated protein lysine acetylases in mycobacteria. J Biol Chem. 2010;285:24313-23 pubmed publisher
    ..Thus, this report not only represents the first demonstration of protein lysine acetylation in mycobacteria but also describes a unique functional interplay between a cyclic nucleotide binding domain and a protein acetyltransferase. ..
  8. de Mendonça Lima L, Picardeau M, Raynaud C, Rauzier J, de la Salmonière Y, Barker L, et al. Erp, an extracellular protein family specific to mycobacteria. Microbiology. 2001;147:2315-20 pubmed
    ..The genome sequence flanking the erp gene includes cell-wall-related ORFs and displays extensive conservation between saprophytic and pathogenic mycobacteria. ..
  9. Wolschendorf F, Mahfoud M, Niederweis M. Porins are required for uptake of phosphates by Mycobacterium smegmatis. J Bacteriol. 2007;189:2435-42 pubmed
    ..However, porins that could mediate the diffusion of phosphates across the OM of M. bovis BCG and Mycobacterium tuberculosis are unknown. ..
  10. Kordulakova J, Gilleron M, Mikusova K, Puzo G, Brennan P, Gicquel B, et al. Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria. J Biol Chem. 2002;277:31335-44 pubmed
    ..smegmatis appears to be dependent on PimA and essential for growth. This work provides the first direct evidence of the essentiality of phosphatidylinositol mannosides for the growth of mycobacteria. ..
  11. Sartain M, Belisle J. N-Terminal clustering of the O-glycosylation sites in the Mycobacterium tuberculosis lipoprotein SodC. Glycobiology. 2009;19:38-51 pubmed publisher
    ..The use of recombinant amino acid substitutions to alter glycosylation sites provided further evidence that glycosylation influences proteolytic processing and ultimately positioning of cell wall proteins. ..
  12. Stephanou N, Gao F, Bongiorno P, Ehrt S, Schnappinger D, Shuman S, et al. Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks. J Bacteriol. 2007;189:5237-46 pubmed
    ..These findings demonstrate that prokaryotic NHEJ is specifically required for DSB repair in late stationary phase and can mediate mutagenic repair of homing endonuclease-generated chromosomal DSBs. ..
  13. Datta S, Krishna R, Ganesh N, Chandra N, Muniyappa K, Vijayan M. Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexes. J Bacteriol. 2003;185:4280-4 pubmed
    ..These observations provide a framework for exploring the known properties of the RecA proteins. ..
  14. Rodrigues L, Ramos J, Couto I, Amaral L, Viveiros M. Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance. BMC Microbiol. 2011;11:35 pubmed publisher
  15. Kendall S, Burgess P, Balhana R, Withers M, Ten Bokum A, Lott J, et al. Cholesterol utilization in mycobacteria is controlled by two TetR-type transcriptional regulators: kstR and kstR2. Microbiology. 2010;156:1362-71 pubmed publisher
    ..The functional significance of the regulon and implications for the control of cholesterol utilization are discussed. ..
  16. Belanger A, Hatfull G. Exponential-phase glycogen recycling is essential for growth of Mycobacterium smegmatis. J Bacteriol. 1999;181:6670-8 pubmed
    ..Thus, in addition to its conventional storage role, glycogen may also serve as a carbon capacitor for glycolysis during the exponential growth of M. smegmatis. ..
  17. Burns K, Liu W, Boshoff H, Dorrestein P, Barry C. Proteasomal protein degradation in Mycobacteria is dependent upon a prokaryotic ubiquitin-like protein. J Biol Chem. 2009;284:3069-75 pubmed publisher
    ..These data therefore establish that, despite differences in both sequence and target linkage, Pup plays an analogous role to ubiquitin in targeting proteins to the proteasome for degradation. ..
  18. Nigou J, Gilleron M, Puzo G. Lipoarabinomannans: from structure to biosynthesis. Biochimie. 2003;85:153-66 pubmed
    ..This offers the possibility of comparative studies that should help in deciphering the structure-function relationships and biosynthesis of these complex molecules in the future. ..
  19. Aniukwu J, Glickman M, Shuman S. The pathways and outcomes of mycobacterial NHEJ depend on the structure of the broken DNA ends. Genes Dev. 2008;22:512-27 pubmed publisher
    ..We conclude that the mechanisms of mycobacterial NHEJ are many and the outcomes depend on the initial structures of the DSBs and the available ensemble of end-processing and end-sealing components, which are not limited to Ku and LigD. ..
  20. Amon J, Bräu T, Grimrath A, Hänssler E, Hasselt K, Höller M, et al. Nitrogen control in Mycobacterium smegmatis: nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the OmpR-type regulator GlnR. J Bacteriol. 2008;190:7108-16 pubmed publisher
    ..The GlnR protein binds specifically to the corresponding promoter sequences and functions as an activator of transcription when cells are subjected to nitrogen starvation. ..
  21. Hümpel A, Gebhard S, Cook G, Berney M. The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress. J Bacteriol. 2010;192:2491-502 pubmed publisher
    ..smegmatis. This study emphasizes the importance of this sigma factor for stationary-phase adaptation and stress response in mycobacteria. ..
  22. Kurthkoti K, Kumar P, Jain R, Varshney U. Important role of the nucleotide excision repair pathway in Mycobacterium smegmatis in conferring protection against commonly encountered DNA-damaging agents. Microbiology. 2008;154:2776-85 pubmed publisher
    ..Taken together with previous observations on NER-deficient M. tuberculosis, these results suggest that NER is an important DNA repair pathway in mycobacteria. ..
  23. Kang C, Nyayapathy S, Lee J, Suh J, Husson R. Wag31, a homologue of the cell division protein DivIVA, regulates growth, morphology and polar cell wall synthesis in mycobacteria. Microbiology. 2008;154:725-35 pubmed publisher
    ..Taken together, these results indicate that Wag31 regulates cell shape and cell wall synthesis in M. tuberculosis through a molecular mechanism by which the activity of Wag31 can be modulated in response to environmental signals. ..
  24. Kaur D, Berg S, Dinadayala P, Gicquel B, Chatterjee D, McNeil M, et al. Biosynthesis of mycobacterial lipoarabinomannan: role of a branching mannosyltransferase. Proc Natl Acad Sci U S A. 2006;103:13664-9 pubmed
    ..The generation of mutants defective in the synthesis of LM/LAM will help define the role of these GPIs in the immunology and pathogenesis of mycobacterial infections and physiology of the organism. ..
  25. Coros A, Callahan B, Battaglioli E, Derbyshire K. The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis. Mol Microbiol. 2008;69:794-808 pubmed publisher
    ..smegmatis DNA transfer and M. tuberculosis virulence. ..
  26. Mishra A, Alderwick L, Rittmann D, Wang C, Bhatt A, Jacobs W, et al. Identification of a novel alpha(1-->6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant. Mol Microbiol. 2008;68:1595-613 pubmed publisher
    ..smegmatis did not result in loss of mycobacterial LM/LAM, indicating a functional redundancy for this enzyme in mycobacteria. ..
  27. Li X, Zhang L, Nikaido H. Efflux pump-mediated intrinsic drug resistance in Mycobacterium smegmatis. Antimicrob Agents Chemother. 2004;48:2415-23 pubmed
    ..Finally, use of isogenic beta-lactamase-deficient strains showed the contribution of LfrA and LfrX to resistance to certain beta-lactams in M. smegmatis. ..
  28. Converse S, Cox J. A protein secretion pathway critical for Mycobacterium tuberculosis virulence is conserved and functional in Mycobacterium smegmatis. J Bacteriol. 2005;187:1238-45 pubmed
    ..M. smegmatis, therefore, represents a powerful system to study the multicomponent Snm secretory machine and to understand the role of this conserved system in mycobacterial biology. ..
  29. Bitter W, Houben E, Bottai D, Brodin P, Brown E, Cox J, et al. Systematic genetic nomenclature for type VII secretion systems. PLoS Pathog. 2009;5:e1000507 pubmed publisher
  30. Sandy J, Mushtaq A, Kawamura A, Sinclair J, Sim E, Noble M. The structure of arylamine N-acetyltransferase from Mycobacterium smegmatis--an enzyme which inactivates the anti-tubercular drug, isoniazid. J Mol Biol. 2002;318:1071-83 pubmed
    ..The structure of M. smegmatis NAT provides a template for modelling the structure of the M. tuberculosis enzyme and for structure-based ligand design as an approach to designing anti-TB drugs. ..
  31. Milano A, Forti F, Sala C, Riccardi G, Ghisotti D. Transcriptional regulation of furA and katG upon oxidative stress in Mycobacterium smegmatis. J Bacteriol. 2001;183:6801-6 pubmed
    ..Transcription from a cloned pkatG, lacking the upstream pfurA region, was not induced upon oxidative stress, suggesting a cis-acting regulatory role of this region. ..
  32. Niederweis M, Ehrt S, Heinz C, Klöcker U, Karosi S, Swiderek K, et al. Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis. Mol Microbiol. 1999;33:933-45 pubmed
    ..These results suggest that MspA is the prototype of a new class of channel-forming proteins. ..
  33. Perrodou E, Deshayes C, Muller J, Schaeffer C, Van Dorsselaer A, Ripp R, et al. ICDS database: interrupted CoDing sequences in prokaryotic genomes. Nucleic Acids Res. 2006;34:D338-43 pubmed
    ..This allows us to estimate the specificity and sensitivity (95 and 82%, respectively) of our program and the efficiency of primer determination. ..
  34. Gong C, Bongiorno P, Martins A, Stephanou N, Zhu H, Shuman S, et al. Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity repair system driven by Ku, ligase D and ligase C. Nat Struct Mol Biol. 2005;12:304-12 pubmed
    ..Another ATP-dependent DNA ligase (LigC) provides a backup mechanism for LigD-independent error-prone repair of blunt-end DSBs. We speculate that NHEJ allows mycobacteria to evade genotoxic host defense. ..
  35. Flint J, Kowalski J, Karnati P, Derbyshire K. The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA transfer in Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 2004;101:12598-603 pubmed
    ..smegmatis. Our results indicate that the M. tuberculosis and M. smegmatis RD1 regions are functionally equivalent and provide a unique perspective on the role of this critical secretion apparatus. ..
  36. Crick D, Schulbach M, Zink E, Macchia M, Barontini S, Besra G, et al. Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis. J Bacteriol. 2000;182:5771-8 pubmed
    ..Thus, the difference in the compositions of the Pol-P of M. smegmatis and M. tuberculosis can be attributed to distinct enzymatic differences between these two organisms. ..
  37. Manina G, Bellinzoni M, Pasca M, Neres J, Milano A, Ribeiro A, et al. Biological and structural characterization of the Mycobacterium smegmatis nitroreductase NfnB, and its role in benzothiazinone resistance. Mol Microbiol. 2010;77:1172-85 pubmed publisher
    ..Although Mycobacterium tuberculosis seems to lack nitroreductases able to inactivate these drugs, our findings are valuable for the design of new BTZ molecules, which may be more effective in vivo. ..
  38. Dhandayuthapani S, Zhang Y, Mudd M, Deretic V. Oxidative stress response and its role in sensitivity to isoniazid in mycobacteria: characterization and inducibility of ahpC by peroxides in Mycobacterium smegmatis and lack of expression in M. aurum and M. tuberculosis. J Bacteriol. 1996;178:3641-9 pubmed
    ..tuberculosis and Mycobacterium bovis INH(r) strains. We propose that mutations activating ahpC expression may contribute to the emergence of INH(r) strains. ..
  39. Daugherty A, Powers K, Standley M, Kim C, Purdy G. Mycobacterium smegmatis RoxY is a repressor of oxyS and contributes to resistance to oxidative stress and bactericidal ubiquitin-derived peptides. J Bacteriol. 2011;193:6824-33 pubmed publisher
    ..Our data indicate that RoxY, OxyS, and AhpD play a role in the mycobacterial oxidative stress response and are important for resistance to host antimicrobial peptides...
  40. Banerjee A, Dubnau E, Quemard A, Balasubramanian V, Um K, Wilson T, et al. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science. 1994;263:227-30 pubmed
    ..These results suggest that InhA is likely a primary target of action for INH and ETH...
  41. Xu H, Hegde S, Blanchard J. Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP. Biochemistry. 2011;50:5883-92 pubmed publisher
    ..Therefore, Pat and the sirtuin-like deacetylase in mycobacteria constitute a reversible acetylation system that regulates the activity of ACS...
  42. Miesel L, Weisbrod T, Marcinkeviciene J, Bittman R, Jacobs W. NADH dehydrogenase defects confer isoniazid resistance and conditional lethality in Mycobacterium smegmatis. J Bacteriol. 1998;180:2459-67 pubmed
    ..tuberculosis complex. The genetic data presented here indicate that defects in NADH oxidation cause all of the mutant traits and that an increase in the NADH/NAD+ ratio confers INH resistance...
  43. Baulard A, Betts J, Engohang Ndong J, Quan S, McAdam R, Brennan P, et al. Activation of the pro-drug ethionamide is regulated in mycobacteria. J Biol Chem. 2000;275:28326-31 pubmed publisher
    ..This study opens up new avenues of research relating to ETH activation in mycobacteria, possibly leading to an improved efficacy of ETH and to the generation of new anti-mycobacterial agents...
  44. Empadinhas N, Albuquerque L, Mendes V, Macedo Ribeiro S, da Costa M. Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase. FEMS Microbiol Lett. 2008;280:195-202 pubmed publisher
    ..It is shown that these genes encode retaining family 81 glycosyltransferases regardless of the low amino acid identity with other known enzymes of this family...
  45. Shi L, Zhou R, Liu Z, Lowary T, Seeberger P, Stocker B, et al. Transfer of the first arabinofuranose residue to galactan is essential for Mycobacterium smegmatis viability. J Bacteriol. 2008;190:5248-55 pubmed publisher
    ..These observations indicate that transfer of the first arabinofuranose residue to galactan is essential for M. smegmatis viability. ..
  46. Balhana R, Swanston S, Coade S, Withers M, Sikder M, Stoker N, et al. bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria. FEMS Microbiol Lett. 2013;345:132-40 pubmed publisher
    ..A conserved operator motif was identified, and binding of purified recombinant M. tuberculosis BkaR to the motif was demonstrated. Analysis of the stoichiometry of binding showed that BkaR binds to the motif as a dimer...
  47. Williams S, Chatterji D. Flexible aspartates propel iron to the ferroxidation sites along pathways stabilized by a conserved arginine in Dps proteins from Mycobacterium smegmatis. Metallomics. 2017;9:685-698 pubmed publisher
  48. Knipfer N, Seth A, Shrader T. Unmarked gene integration into the chromosome of Mycobacterium smegmatis via precise replacement of the pyrF gene. Plasmid. 1997;37:129-40 pubmed publisher
    ..Two pyrF-containing plasmids, designed to exploit the new delta pyrF strain, have been constructed and their possible applications to problems in mycobacteriology are discussed...
  49. Banerjee A, Adolph R, Gopalakrishnapai J, Kleinboelting S, Emmerich C, Steegborn C, et al. A universal stress protein (USP) in mycobacteria binds cAMP. J Biol Chem. 2015;290:12731-43 pubmed publisher
    ..This family of USPs is conserved in all mycobacteria, and we suggest that they serve as "sinks" for cAMP, making this second messenger available for downstream effectors as and when ATP levels are altered in the cell. ..
  50. Roy S, Saraswathi R, Gupta S, Sekar K, Chatterji D, Vijayan M. Role of N and C-terminal tails in DNA binding and assembly in Dps: structural studies of Mycobacterium smegmatis Dps deletion mutants. J Mol Biol. 2007;370:752-67 pubmed publisher
    ..This disorder appears to result in the formation of a trimeric species of the N-terminal deletion mutant contrary to the indication provided by the native structure. The ferroxidation site is intact in the mutants...
  51. Carroll J, Pastuszak I, Edavana V, Pan Y, Elbein A. A novel trehalase from Mycobacterium smegmatis - purification, properties, requirements. FEBS J. 2007;274:1701-14 pubmed publisher
    ..The enzyme has a pH optimum of 7.1, and is active in various buffers, as long as inorganic phosphate and Mg(2+) are present. Glucose was the only product produced by the trehalase in the presence of either phosphate or arsenate...
  52. Sharma R, Zaveri A, Gopalakrishnapai J, Srinath T, Thiruneelakantan S, Varshney U, et al. Paralogous cAMP receptor proteins in Mycobacterium smegmatis show biochemical and functional divergence. Biochemistry. 2014;53:7765-76 pubmed publisher
    ..This gene duplication event has subsequently led to the evolution of two proteins whose biochemical differences translate to differential gene regulation, thus catering to the specific needs of the organism. ..
  53. Yang Q, Huang F, Hu L, He Z. Physical and functional interactions between 3-methyladenine DNA glycosylase and topoisomerase I in mycobacteria. Biochemistry (Mosc). 2012;77:378-87 pubmed publisher
    ..Several mutations in MAG that led to the loss of its interaction with and activity regulation of TopA were also characterized. The results of this study further elucidate glycosylase regulation in both M. smegmatis and M. tuberculosis...
  54. Carrère Kremer S, Blaise M, Singh V, Alibaud L, Tuaillon E, Halloum I, et al. A new dehydratase conferring innate resistance to thiacetazone and intra-amoebal survival of Mycobacterium smegmatis. Mol Microbiol. 2015;96:1085-102 pubmed publisher
    ..This in vivo growth defect was fully restored upon complementation with catalytically active MSMEG_6754 or HadABC, indicating that MSMEG_6754 plays a critical role in the survival of M. smegmatis within the environmental host. ..
  55. Tschumi A, Grau T, Albrecht D, Rezwan M, Antelmann H, Sander P. Functional analyses of mycobacterial lipoprotein diacylglyceryl transferase and comparative secretome analysis of a mycobacterial lgt mutant. J Bacteriol. 2012;194:3938-49 pubmed publisher
    ..We identify the major Lgt in M. smegmatis, show that lipoproteins lacking the lipid anchor are secreted into the culture filtrate, and demonstrate that M. tuberculosis lgt is essential and thus a validated drug target...
  56. Pavelka M, Jacobs W. Biosynthesis of diaminopimelate, the precursor of lysine and a component of peptidoglycan, is an essential function of Mycobacterium smegmatis. J Bacteriol. 1996;178:6496-507 pubmed
    ..The evidence presented here indicates that the aspartate pathway is essential to M. smegmatis and that DAP is the essential product of this pathway...
  57. Yang J, Liu Y, Bi J, Cai Q, Liao X, Li W, et al. Structural basis for targeting the ribosomal protein S1 of Mycobacterium tuberculosis by pyrazinamide. Mol Microbiol. 2015;95:791-803 pubmed publisher
    ..Our findings provide insights into the mode of action of PZA and molecular basis of PZA resistance associated with RpsA mutations. ..
  58. Glover R, Kriakov J, Garforth S, Baughn A, Jacobs W. The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. J Bacteriol. 2007;189:5495-503 pubmed publisher
    ..A DNA motif consisting of an inverted repeat was identified in each of the promoters bound by RegX3 approximately P. Based upon our findings, we propose a model for P(i)-regulated gene expression mediated by SenX3-RegX3 in mycobacteria...
  59. Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, et al. Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications. J Mol Biol. 2003;331:385-93 pubmed
    ..Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB...
  60. Bhat A, Leelaram M, Hegde S, Nagaraja V. Deciphering the distinct role for the metal coordination motif in the catalytic activity of Mycobacterium smegmatis topoisomerase I. J Mol Biol. 2009;393:788-802 pubmed publisher
    ..Thus, although architecturally and mechanistically similar to topoisomerase I from E. coli, the metal coordination pattern of the mycobacterial enzyme is distinct, opening up avenues to exploit the enzyme to develop inhibitors...
  61. Rawat M, Uppal M, Newton G, Steffek M, Fahey R, Av Gay Y. Targeted mutagenesis of the Mycobacterium smegmatis mca gene, encoding a mycothiol-dependent detoxification protein. J Bacteriol. 2004;186:6050-8 pubmed publisher
    ..Mutants disrupted in genes belonging to MSH biosynthesis are also more susceptible to streptomycin, providing further evidence that Mca detoxifies streptomycin in the mycobacterial cell in an MSH-dependent manner...
  62. Hutter B, Dick T. Increased alanine dehydrogenase activity during dormancy in Mycobacterium smegmatis. FEMS Microbiol Lett. 1998;167:7-11 pubmed
    ..As alanine synthesis is coupled to NADH oxidation, we propose that the induction of alanine dehydrogenase activity might also support the maintenance of the NAD pool when oxygen as a terminal electron acceptor becomes limiting...
  63. Hett E, Chao M, Rubin E. Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria. PLoS Pathog. 2010;6:e1001020 pubmed publisher
    ..These data reveal a post-translational mechanism for regulating cell wall hydrolysis and synthesis through protein-protein interactions between enzymes with antagonistic functions...
  64. Pi N, Hoang M, Gao H, Mougous J, Bertozzi C, Leary J. Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry. Anal Biochem. 2005;341:94-104 pubmed publisher
    ..To our knowledge, this is the first detailed mechanistic data reported for Stf0, which further demonstrates the power of mass spectrometry in elucidating the reaction pathway and catalytic mechanism of promising enzymatic systems...
  65. Tersa M, Raich L, Albesa Jové D, Trastoy B, Prandi J, Gilleron M, et al. The Molecular Mechanism of Substrate Recognition and Catalysis of the Membrane Acyltransferase PatA from Mycobacteria. ACS Chem Biol. 2018;13:131-140 pubmed publisher
  66. Deshayes C, Bach H, Euphrasie D, Attarian R, Coureuil M, Sougakoff W, et al. MmpS4 promotes glycopeptidolipids biosynthesis and export in Mycobacterium smegmatis. Mol Microbiol. 2010;78:989-1003 pubmed publisher
    ..We suggest that MmpS proteins facilitate lipid biosynthesis by acting as a scaffold for coupled biosynthesis and transport machinery...
  67. Yu S, Fiss E, Jacobs W. Analysis of the exochelin locus in Mycobacterium smegmatis: biosynthesis genes have homology with genes of the peptide synthetase family. J Bacteriol. 1998;180:4676-85 pubmed
    ..The fxbB and fxbC genes encode large proteins of 257 and 497 kDa, respectively, which are highly homologous to peptide synthetases, indicating that exochelin biosynthesis occurs by a nonribosomal mechanism...
  68. Li H, Graupner M, Xu H, White R. CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii. Biochemistry. 2003;42:9771-8 pubmed publisher
    ..Evidence for the occurrence of this intermediate is presented. A reaction mechanism for the enzyme is proposed and compared with other members of the ADP-forming amide bond ligase family...
  69. Newton G, Ta P, Bzymek K, Fahey R. Biochemistry of the initial steps of mycothiol biosynthesis. J Biol Chem. 2006;281:33910-20 pubmed publisher
    ..This demonstrates the presence of a myo-inositol transporter and a kinase that generates 1L-myo-inositol 1-phosphate. The biochemical pathway of mycothiol biosynthesis is now fully elucidated...
  70. Plocinska R, Purushotham G, Sarva K, Vadrevu I, Pandeeti E, Arora N, et al. Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression. J Biol Chem. 2012;287:23887-99 pubmed publisher
    ..We propose that MtrB septal association is a necessary feature of MtrB activation that promotes MtrA phosphorylation and MtrA regulon expression...
  71. Huang F, He Z. Characterization of a conserved interaction between DNA glycosylase and ParA in Mycobacterium smegmatis and M. tuberculosis. PLoS ONE. 2012;7:e38276 pubmed publisher
    ..tuberculosis and M. smegmatis. Our findings provide important new insights into the regulatory mechanism of cell growth and division in mycobacteria...
  72. Honor N, Cole S. Streptomycin resistance in mycobacteria. Antimicrob Agents Chemother. 1994;38:238-42 pubmed
    ..avium, M. gordonae, and M. szulgai, that are naturally resistant to streptomycin. This suggests that permeability barriers may be responsible for the resistance to the antibiotic...
  73. Rybniker J, Nowag A, van Gumpel E, Nissen N, Robinson N, Plum G, et al. Insights into the function of the WhiB-like protein of mycobacteriophage TM4--a transcriptional inhibitor of WhiB2. Mol Microbiol. 2010;77:642-57 pubmed publisher
    ..Thus, we provide substantial evidence supporting the hypothesis of viral and bacterial WhiB proteins being important Fe-S containing transcriptional regulators with DNA-binding capability...
  74. Lu L, Sun Q, Fan X, Zhong Y, Yao Y, Zhao G. Mycobacterial MazG is a novel NTP pyrophosphohydrolase involved in oxidative stress response. J Biol Chem. 2010;285:28076-85 pubmed publisher
    ..Our work implicates a role for the MazG activity in the virulence of M. tuberculosis...
  75. Nguyen H, Wolff K, Cartabuke R, Ogwang S, Nguyen L. A lipoprotein modulates activity of the MtrAB two-component system to provide intrinsic multidrug resistance, cytokinetic control and cell wall homeostasis in Mycobacterium. Mol Microbiol. 2010;76:348-64 pubmed publisher
    ..These results support a model in which LpqB, MtrB and MtrA form a three-component system that co-ordinates cytokinetic and cell wall homeostatic processes...
  76. García Fernández J, Papavinasasundaram K, Galán B, Sassetti C, Garcia J. Unravelling the pleiotropic role of the MceG ATPase in Mycobacterium smegmatis. Environ Microbiol. 2017;19:2564-2576 pubmed publisher
    ..Several ?MceG phenotypes were recapitulated in a strain lacking only the unique C-terminal region of MceG, suggesting an important functional or regulatory function for this domain. ..
  77. Li S, Kang J, Yu W, Zhou Y, Zhang W, Xin Y, et al. Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556. PLoS ONE. 2012;7:e42769 pubmed publisher
    ..These results demonstrated that MSMEG_1556 is essential for growth of M. smegmatis. This study provided evidence that GlmM enzyme could be as a potential target for developing anti-tuberculosis drugs...