Mycobacterium bovis AF2122/97

Summary

Alias: Mycobacterium bovis str. AF2122/97, Mycobacterium bovis subsp. bovis AF2122/97, Mycobacterium bovis subsp. bovis str. AF2122/97

Top Publications

  1. Constant P, Perez E, Malaga W, Lanéelle M, Saurel O, Daffe M, et al. Role of the pks15/1 gene in the biosynthesis of phenolglycolipids in the Mycobacterium tuberculosis complex. Evidence that all strains synthesize glycosylated p-hydroxybenzoic methyl esters and that strains devoid of phenolglycolipids harbor a frames. J Biol Chem. 2002;277:38148-58 pubmed
    ..These data indicate that Pks15/1 is involved in the elongation of p-hydroxybenzoic acid to give p-hydroxyphenylalkanoates, which in turn are converted, presumably by the PpsA-E synthase, to phenolphthiocerol derivatives. ..
  2. Mehta R, Pearson J, Mahajan S, Nath A, Hickey M, Sherman D, et al. Adenylylation and catalytic properties of Mycobacterium tuberculosis glutamine synthetase expressed in Escherichia coli versus mycobacteria. J Biol Chem. 2004;279:22477-82 pubmed
    ..This parallels the low adenylylation states observed for GS from mycobacteria and suggests the intriguing possibility that adenylylation in the pathogenic versus non-pathogenic mycobacteria is differentially regulated. ..
  3. Matsuo T, Matsuo H, Ohara N, Matsumoto S, Kitaura H, Mizuno A, et al. Cloning and sequencing of an MPB70 homologue corresponding to MPB83 from Mycobacterium bovis BCG. Scand J Immunol. 1996;43:483-9 pubmed
    ..It was speculated that the gene the authors characterized probably corresponded to the mpb83 gene. ..
  4. Kim J, Kim J, Kim S, Bai G, Cho S, Kang S, et al. Cloning and sequencing of the secY gene homolog from Mycobacterium bovis BCG. Biochem Mol Biol Int. 1997;43:391-8 pubmed
    ..Comparative analyses of the deduced amino acid sequence of additional partial ORF revealed strong similarity to the known adenylate kinases. ..
  5. Ohara N, Kimura M, Wada N, Yamada T. Cloning and sequencing of the gene encoding the ribosomal L7/L12-like protein of Mycobacterium bovis BCG. Nucleic Acids Res. 1993;21:3579 pubmed
  6. Iwanaga S, Ohara N, Kariu T, Kimura M, Yamasaki N, Yamada T. Cloning and nucleotide sequence of the gene cluster encoding ribosomal proteins S12 and S7 from Mycobacterium bovis BCG. Biochem Mol Biol Int. 1995;36:209-18 pubmed
    ..However, the intercistronic region between S12 and S7 genes, which plays an important role for autoregulation for the str operon in E. coli, is completely absent in M. bovis. ..
  7. Kim J, Lee H, Lim J, Kim S, Cho S, Jeong W, et al. Molecular cloning and characterization of Mycobacterium bovis BCG pcp gene encoding pyrrolidone carboxyl peptidase. Mol Cells. 2001;12:347-52 pubmed
    ..A multiple sequence alignment revealed highly conserved domains. The BCG pcp gene was overexpressed in Escherichia coli. The Pcp was purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay. ..
  8. Bigi F, Espitia C, Alito A, Zumarraga M, Romano M, Cravero S, et al. A novel 27 kDa lipoprotein antigen from Mycobacterium bovis. Microbiology. 1997;143 ( Pt 11):3599-605 pubmed publisher
    ..bovis/M. tuberculosis has been identified. The results presented here and elsewhere suggest that mycobacterial lipoproteins should be considered in the design of new recombinant vaccines and diagnostic methods...
  9. Rainwater D, Kolattukudy P. Fatty acid biosynthesis in Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guérin. Purification and characterization of a novel fatty acid synthase, mycocerosic acid synthase, which elongates n-fatty acyl-CoA with methylmalonyl-CoA. J Biol Chem. 1985;260:616-23 pubmed
    ..It is concluded that the mycocerosic acid synthase is a multifunctional enzyme similar to the well-characterized multifunctional fatty acid synthases except for the substrate specificity...
  10. Dubnau E, Soares S, Huang T, Jacobs W. Overproduction of mycobacterial ribosomal protein S13 induces catalase/peroxidase activity and hypersensitivity to isoniazid in Mycobacterium smegmatis. Gene. 1996;170:17-22 pubmed
    ..The overproduction of S13 may induce a stress response, resulting in increased expression of katG (encoding Kat/Prx) in Ms, thereby causing hypersensitivity to INH...

Detail Information

Publications101 found, 100 shown here

  1. Constant P, Perez E, Malaga W, Lanéelle M, Saurel O, Daffe M, et al. Role of the pks15/1 gene in the biosynthesis of phenolglycolipids in the Mycobacterium tuberculosis complex. Evidence that all strains synthesize glycosylated p-hydroxybenzoic methyl esters and that strains devoid of phenolglycolipids harbor a frames. J Biol Chem. 2002;277:38148-58 pubmed
    ..These data indicate that Pks15/1 is involved in the elongation of p-hydroxybenzoic acid to give p-hydroxyphenylalkanoates, which in turn are converted, presumably by the PpsA-E synthase, to phenolphthiocerol derivatives. ..
  2. Mehta R, Pearson J, Mahajan S, Nath A, Hickey M, Sherman D, et al. Adenylylation and catalytic properties of Mycobacterium tuberculosis glutamine synthetase expressed in Escherichia coli versus mycobacteria. J Biol Chem. 2004;279:22477-82 pubmed
    ..This parallels the low adenylylation states observed for GS from mycobacteria and suggests the intriguing possibility that adenylylation in the pathogenic versus non-pathogenic mycobacteria is differentially regulated. ..
  3. Matsuo T, Matsuo H, Ohara N, Matsumoto S, Kitaura H, Mizuno A, et al. Cloning and sequencing of an MPB70 homologue corresponding to MPB83 from Mycobacterium bovis BCG. Scand J Immunol. 1996;43:483-9 pubmed
    ..It was speculated that the gene the authors characterized probably corresponded to the mpb83 gene. ..
  4. Kim J, Kim J, Kim S, Bai G, Cho S, Kang S, et al. Cloning and sequencing of the secY gene homolog from Mycobacterium bovis BCG. Biochem Mol Biol Int. 1997;43:391-8 pubmed
    ..Comparative analyses of the deduced amino acid sequence of additional partial ORF revealed strong similarity to the known adenylate kinases. ..
  5. Ohara N, Kimura M, Wada N, Yamada T. Cloning and sequencing of the gene encoding the ribosomal L7/L12-like protein of Mycobacterium bovis BCG. Nucleic Acids Res. 1993;21:3579 pubmed
  6. Iwanaga S, Ohara N, Kariu T, Kimura M, Yamasaki N, Yamada T. Cloning and nucleotide sequence of the gene cluster encoding ribosomal proteins S12 and S7 from Mycobacterium bovis BCG. Biochem Mol Biol Int. 1995;36:209-18 pubmed
    ..However, the intercistronic region between S12 and S7 genes, which plays an important role for autoregulation for the str operon in E. coli, is completely absent in M. bovis. ..
  7. Kim J, Lee H, Lim J, Kim S, Cho S, Jeong W, et al. Molecular cloning and characterization of Mycobacterium bovis BCG pcp gene encoding pyrrolidone carboxyl peptidase. Mol Cells. 2001;12:347-52 pubmed
    ..A multiple sequence alignment revealed highly conserved domains. The BCG pcp gene was overexpressed in Escherichia coli. The Pcp was purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay. ..
  8. Bigi F, Espitia C, Alito A, Zumarraga M, Romano M, Cravero S, et al. A novel 27 kDa lipoprotein antigen from Mycobacterium bovis. Microbiology. 1997;143 ( Pt 11):3599-605 pubmed publisher
    ..bovis/M. tuberculosis has been identified. The results presented here and elsewhere suggest that mycobacterial lipoproteins should be considered in the design of new recombinant vaccines and diagnostic methods...
  9. Rainwater D, Kolattukudy P. Fatty acid biosynthesis in Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guérin. Purification and characterization of a novel fatty acid synthase, mycocerosic acid synthase, which elongates n-fatty acyl-CoA with methylmalonyl-CoA. J Biol Chem. 1985;260:616-23 pubmed
    ..It is concluded that the mycocerosic acid synthase is a multifunctional enzyme similar to the well-characterized multifunctional fatty acid synthases except for the substrate specificity...
  10. Dubnau E, Soares S, Huang T, Jacobs W. Overproduction of mycobacterial ribosomal protein S13 induces catalase/peroxidase activity and hypersensitivity to isoniazid in Mycobacterium smegmatis. Gene. 1996;170:17-22 pubmed
    ..The overproduction of S13 may induce a stress response, resulting in increased expression of katG (encoding Kat/Prx) in Ms, thereby causing hypersensitivity to INH...
  11. Nair J, Rouse D, Morris S. Nucleotide sequence analysis of the ribosomal S12 gene of Mycobacterium intracellulare. Nucleic Acids Res. 1993;21:1039 pubmed
  12. Azad A, Sirakova T, Fernandes N, Kolattukudy P. Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria. J Biol Chem. 1997;272:16741-5 pubmed
    ..With the identification of the pps gene clusters in both M. tuberculosis and M. leprae, it should be possible to test the postulated roles of these unique lipids in tuberculosis and leprosy...
  13. Yamaguchi R, Matsuo K, Yamazaki A, Nagai S, Terasaka K, Yamada T. Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression. FEBS Lett. 1988;240:115-7 pubmed
    ..This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57. ..
  14. Radford A, Duffield B, Plackett P. Cloning of a species-specific antigen of Mycobacterium bovis. Infect Immun. 1988;56:921-5 pubmed
    ..Apart from this deletion, there were seven other variations between the cloned sequence and that deduced from M. bovis BCG MPB70. ..
  15. Thole J, Keulen W, De Bruyn J, Kolk A, Groothuis D, Berwald L, et al. Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12. Infect Immun. 1987;55:1466-75 pubmed
    ..bovis BCG or MbaA showed a delayed-type hypersensitivity reaction after challenge with purified MbaA, supporting the previously observed strong reactivity of human T-cell clones with this, for mycobacteria, common antigen...
  16. Sharma V, Sharma S, Hoener zu Bentrup K, McKinney J, Russell D, Jacobs W, et al. Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis. Nat Struct Biol. 2000;7:663-8 pubmed publisher
    ..The structure of a C191S mutant of the enzyme with the inhibitor 3-nitropropionate provides further insight into the reaction mechanism...
  17. Tripathi D, Chandra H, Bhatnagar R. Poly-L-glutamate/glutamine synthesis in the cell wall of Mycobacterium bovis is regulated in response to nitrogen availability. BMC Microbiol. 2013;13:226 pubmed publisher
    ..This study demonstrate that the nitrogen availability not only regulates GS expression and activity in M. bovis but also affects cell surface properties by modulating synthesis of PLG. ..
  18. Madhusudan K, Nagaraja V. Mycobacterium smegmatis DNA gyrase: cloning and overexpression in Escherichia coli. Microbiology. 1995;141 ( Pt 12):3029-37 pubmed
    ..This activity was ATP-dependent and novobiocin-sensitive. The identity of the genes was also confirmed by complementation analysis. ..
  19. Rosenkrands I, Rasmussen P, Carnio M, Jacobsen S, Theisen M, Andersen P. Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. Infect Immun. 1998;66:2728-35 pubmed
    ..Interspecies analysis by immunoblotting and PCR demonstrated that CFP29 is widely distributed in mycobacterial species...
  20. Choi K, Kendrick N, Daniels L. Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis. J Bacteriol. 2002;184:2420-8 pubmed
    ..bovis BCG sequence (approximately 95,000 Da), as well as three other histidine-tagged proteins of significantly smaller size, which are thought to be proteolysis products of the FbiC fusion protein...
  21. Walburger A, Koul A, Ferrari G, Nguyen L, Prescianotto Baschong C, Huygen K, et al. Protein kinase G from pathogenic mycobacteria promotes survival within macrophages. Science. 2004;304:1800-4 pubmed publisher
  22. Collins D, Stephens D. Identification of an insertion sequence, IS1081, in Mycobacterium bovis. FEMS Microbiol Lett. 1991;67:11-5 pubmed
    ..IS1081 may be useful for genetic manipulations and for developing a diagnostic test for bovine tuberculosis based on the polymerase chain reaction...
  23. McKenzie K, Adams E, Britton W, Garsia R, Basten A. Sequence and immunogenicity of the 70-kDa heat shock protein of Mycobacterium leprae. J Immunol. 1991;147:312-9 pubmed
    ..leprae recombinant proteins were lower than to mAb affinity-purified BCG p70. Thus, the M. leprae 70-kDa heat shock protein elicits T and B cell responses in subjects exposed to mycobacteria, despite its homology with the human hsp70. ..
  24. Hance A, Grandchamp B, Levy Frebault V, Lecossier D, Rauzier J, Bocart D, et al. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol Microbiol. 1989;3:843-9 pubmed
    ..These results suggest that this approach may prove useful in the early diagnosis of mycobacterial infection. ..
  25. Kapur V, Li L, Hamrick M, Plikaytis B, Shinnick T, Telenti A, et al. Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing. Arch Pathol Lab Med. 1995;119:131-8 pubmed
    ..Taken together, the data demonstrate the feasibility of mycobacterial species identification and potential to identify mutations associated with antimicrobial resistance in less than 48 hours. ..
  26. Gordon S, Brosch R, Billault A, Garnier T, Eiglmeier K, Cole S. Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays. Mol Microbiol. 1999;32:643-55 pubmed
    ..These deletions affect a further seven genes, including a fourth phospholipase, plcD. In summary, the insertions and deletions described here have important implications for our understanding of the evolution of the tubercle complex...
  27. Wilson T, de Lisle G, Collins D. Effect of inhA and katG on isoniazid resistance and virulence of Mycobacterium bovis. Mol Microbiol. 1995;15:1009-15 pubmed
    ..Integration of a functional katG gene into the most resistant strain restored full virulence. This clearly established that katG is a virulence factor for M. bovis and that mutation of the inhA gene has no effect on virulence...
  28. Kenney T, Churchward G. Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycin. J Bacteriol. 1994;176:6153-6 pubmed
    ..Forty-six percent of the mutations (13 of 28) were due to a transition changing an AAG Lys codon to an AGG Arg codon, with eight changes at codon 43 and five at codon 88. ..
  29. Ohara N, Kimura M, Higashi Y, Yamada T. Isolation and amino acid sequence of the 30S ribosomal protein S19 from Mycobacterium bovis BCG. FEBS Lett. 1993;331:9-14 pubmed
    ..coli, and B. stearothermophilus, respectively; 33% of the residues of MboS19 were identical to those in the archaebacterial ribosomal protein HmaS19. ..
  30. Kim J, Kim J, Choe Y. Cloning and sequencing of the pyrG encoding cytidine 5'-triphosphate synthetase from Mycobacterium bovis BCG. Biochem Mol Biol Int. 1997;43:925-33 pubmed
  31. Nagabhushanam V, Praszkier J, Cheers C. Molecular and immunological characterization of Mycobacterium avium 65 kDa heat shock protein (Hsp65). Immunol Cell Biol. 2001;79:454-61 pubmed
    ..Therefore, the findings indicate that Hsp65 is not a dominant T-cell antigen during M. avium infection. ..
  32. Malone K, Farrell D, Stuber T, Schubert O, Aebersold R, Robbe Austerman S, et al. Updated Reference Genome Sequence and Annotation of Mycobacterium bovis AF2122/97. Genome Announc. 2017;5: pubmed publisher
    ..The update includes 42 new coding sequences (CDSs), 14 modified annotations, 26 single-nucleotide polymorphism (SNP) corrections, and disclosure that the RD900 locus, previously described as absent from the genome, is in fact present. ..
  33. Timm J, Van Rompaey I, Tricot C, Massaer M, Haeseleer F, Fauconnier A, et al. Molecular cloning, characterization and purification of ornithine carbamoyltransferase from Mycobacterium bovis BCG. Mol Gen Genet. 1992;234:475-80 pubmed
    ..The native enzyme has an estimated molecular mass of 110 kDa, suggesting a trimeric structure as is the case for most of the anabolic OTCases known from various organisms. ..
  34. Matsuo T, Matsumoto S, Ohara N, Kitaura H, Mizuno A, Yamada T. Differential transcription of the MPB70 genes in two major groups of Mycobacterium bovis BCG substrains. Microbiology. 1995;141 ( Pt 7):1601-7 pubmed publisher
    ..On the basis of these results, the difference in the secretion of the MPB70 protein between BCG Tokyo and Pasteur was attributed to differential transcription efficiencies...
  35. Aldovini A, Husson R, Young R. The uraA locus and homologous recombination in Mycobacterium bovis BCG. J Bacteriol. 1993;175:7282-9 pubmed
    ..These results have implications for understanding the role of mycobacterial genes in disease pathogenesis and for the genetic engineering of improved mycobacterial vaccines. ..
  36. Arruda S, Bomfim G, Knights R, Huima Byron T, Riley L. Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells. Science. 1993;261:1454-7 pubmed
    ..This capacity to gain entry into mammalian cells and survive inside the macrophage was localized to two distinct loci on the cloned M. tuberculosis DNA fragment...
  37. Han M, Son M, Lee S, Kim J, Huh J, Kim J, et al. Molecular cloning of the leuB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis. Biochem Mol Biol Int. 1997;41:657-63 pubmed
    ..011 bp encoding isopropylmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp. ..
  38. Chambers M, Whelan A, Spallek R, Singh M, Coddeville B, Guerardel Y, et al. Non-acylated Mycobacterium bovis glycoprotein MPB83 binds to TLR1/2 and stimulates production of matrix metalloproteinase 9. Biochem Biophys Res Commun. 2010;400:403-8 pubmed publisher
    ..Direct interaction of RecMPB83 with TLR2 was demonstrated by surface plasmon-resonance. MPB83 may act as a virulence factor through TLR2 mediated induction of MMP-9...
  39. St landre M, Bosseloir Y, De Bruyn J, Maes P, Content J. Cloning and sequence analysis of the gene encoding an NADP-dependent alcohol dehydrogenase in Mycobacterium bovis BCG. Gene. 1992;121:79-86 pubmed
    ..Of the 22 strictly conserved residues in this group, 19 are also conserved in M. bovis BCG ADH (BCGADH)...
  40. Radford A, Wood P, Billman Jacobe H, Geysen H, Mason T, Tribbick G. Epitope mapping of the Mycobacterium bovis secretory protein MPB70 using overlapping peptide analysis. J Gen Microbiol. 1990;136:265-72 pubmed publisher
  41. Collins M, Patki A, Wall S, Nolan A, Goodger J, Woodward M, et al. Cloning and characterization of the gene for the '19 kDa' antigen of Mycobacterium bovis. J Gen Microbiol. 1990;136:1429-36 pubmed publisher
    ..This sequence is identical to that, independently determined, of a gene from M. tuberculosis, usually referred to as the 19 kDa antigen. The reasons for the apparent size discrepancies are discussed...
  42. Yamaguchi R, Matsuo K, Yamazaki A, Abe C, Nagai S, Terasaka K, et al. Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG. Infect Immun. 1989;57:283-8 pubmed
    ..The strict specificity of MPB64 could be applied to immunodiagnosis of tuberculosis...
  43. Terasaka K, Yamaguchi R, Matsuo K, Yamazaki A, Nagai S, Yamada T. Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG. FEMS Microbiol Lett. 1989;49:273-6 pubmed
    ..The SP displayed a characteristic feature of an Ala-rich property which would be efficient in a SP function...
  44. Harboe M, Nagai S, Patarroyo M, Torres M, Ramirez C, Cruz N. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG. Infect Immun. 1986;52:293-302 pubmed
  45. Heym B, Alzari P, Honor N, Cole S. Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis. Mol Microbiol. 1995;15:235-45 pubmed
    ..The second group comprises a frequently occurring amino acid substitution and a single mutation that are both located in the C-terminal domain but do not noticeably alter either enzyme activity or heat stability...
  46. Mahairas G, Sabo P, Hickey M, Singh D, Stover C. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. J Bacteriol. 1996;178:1274-82 pubmed
    ..These findings may be applicable to the rational design of a new attenuated tuberculosis vaccine and the development of new diagnostic tests to distinguish BCG vaccination from tuberculosis infection...
  47. Wilson T, Collins D. ahpC, a gene involved in isoniazid resistance of the Mycobacterium tuberculosis complex. Mol Microbiol. 1996;19:1025-34 pubmed
    ..Increased expression of AhpC may account for some of the INH resistance of strains of the M. tuberculosis complex...
  48. Dubnau E, Lan elle M, Soares S, B nichou A, Vaz T, Prom D, et al. Mycobacterium bovis BCG genes involved in the biosynthesis of cyclopropyl keto- and hydroxy-mycolic acids. Mol Microbiol. 1997;23:313-22 pubmed
    ..Furthermore, there is a perfect match between the structures of the keto- and the hydroxy-mycolic acids. We propose a biosynthetic model in which there is a direct relationship between these two types of mycolic acid...
  49. Sander P, Prammananan T, Meier A, Frischkorn K, B ttger E. The role of ribosomal RNAs in macrolide resistance. Mol Microbiol. 1997;26:469-80 pubmed
    ..recessivity and gene dosage effects in eubacterial ribosomal nucleic acids to be addressed experimentally in vivo...
  50. Miesel L, Weisbrod T, Marcinkeviciene J, Bittman R, Jacobs W. NADH dehydrogenase defects confer isoniazid resistance and conditional lethality in Mycobacterium smegmatis. J Bacteriol. 1998;180:2459-67 pubmed
    ..tuberculosis complex. The genetic data presented here indicate that defects in NADH oxidation cause all of the mutant traits and that an increase in the NADH/NAD+ ratio confers INH resistance...
  51. Yuan Y, Zhu Y, Crane D, Barry C. The effect of oxygenated mycolic acid composition on cell wall function and macrophage growth in Mycobacterium tuberculosis. Mol Microbiol. 1998;29:1449-58 pubmed
    ..These results establish a critical role for mycolate composition in proper cell wall function during the growth of MTB in vivo...
  52. Behr M, Schroeder B, Brinkman J, Slayden R, Barry C. A point mutation in the mma3 gene is responsible for impaired methoxymycolic acid production in Mycobacterium bovis BCG strains obtained after 1927. J Bacteriol. 2000;182:3394-9 pubmed
    ..These findings indicate that a point mutation in mma3 occurred between 1927 and 1931, and that this mutant population became the dominant clone of BCG at the Pasteur Institute...
  53. Silva P, Bigi F, Santangelo M, Romano M, Mart n C, Cataldi A, et al. Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2001;45:800-4 pubmed publisher
    ..M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria...
  54. Scarsdale J, Kazanina G, He X, Reynolds K, Wright H. Crystal structure of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III. J Biol Chem. 2001;276:20516-22 pubmed publisher
    ..This mtFabH presents a new target for structure-based design of novel antimycobacterial agents...
  55. Steyn A, Collins D, Hondalus M, Jacobs W, Kawakami R, Bloom B. Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth. Proc Natl Acad Sci U S A. 2002;99:3147-52 pubmed publisher
    ..bovis and M. tuberculosis whiB3 genes in pathogenesis generated in different animal models. We propose that M. tuberculosis WhiB3 functions as a transcription factor regulating genes that influence the immune response of the host...
  56. Prabhakar S, Mishra A, Singhal A, Katoch V, Thakral S, Tyagi J, et al. Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis. J Clin Microbiol. 2004;42:2724-32 pubmed publisher
    ..tuberculosis and M. bovis...
  57. Temmerman S, Place S, Debrie A, Locht C, Mascart F. Effector functions of heparin-binding hemagglutinin-specific CD8+ T lymphocytes in latent human tuberculosis. J Infect Dis. 2005;192:226-32 pubmed publisher
  58. Ad kambi T, Berger P, Raoult D, Drancourt M. rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol. 2006;56:133-43 pubmed publisher
  59. Mathur M, Kolattukudy P. Molecular cloning and sequencing of the gene for mycocerosic acid synthase, a novel fatty acid elongating multifunctional enzyme, from Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guerin. J Biol Chem. 1992;267:19388-95 pubmed
    ..This result is consistent with the report that mycocerosic acid is present only in pathogenic mycobacteria...
  60. Ohara N, Kitaura H, Hotokezaka H, Nishiyama T, Wada N, Matsumoto S, et al. Characterization of the gene encoding the MPB51, one of the major secreted protein antigens of Mycobacterium bovis BCG, and identification of the secreted protein closely related to the fibronectin binding 85 complex. Scand J Immunol. 1995;41:433-42 pubmed
    ..Two-dimensional electrophoresis of culture fluids of BCG and Western blotting indicated the existence of the other novel protein(s) which strongly cross-reacted with the alpha antigen (85B) and MPB51...
  61. Cirillo J, Weisbrod T, Pascopella L, Bloom B, Jacobs W. Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria. Mol Microbiol. 1994;11:629-39 pubmed
    ..Primer extension analysis revealed that the only transcriptional start in this region is found 5' of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon...
  62. Honor N, Cole S. Streptomycin resistance in mycobacteria. Antimicrob Agents Chemother. 1994;38:238-42 pubmed
    ..avium, M. gordonae, and M. szulgai, that are naturally resistant to streptomycin. This suggests that permeability barriers may be responsible for the resistance to the antibiotic...
  63. Banerjee A, Dubnau E, Quemard A, Balasubramanian V, Um K, Wilson T, et al. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science. 1994;263:227-30 pubmed
    ..These results suggest that InhA is likely a primary target of action for INH and ETH...
  64. Agranoff D, Krishna S. Metal ion homeostasis and intracellular parasitism. Mol Microbiol. 1998;28:403-12 pubmed
    ..Mycobacterial homologues have recently been identified by genomic analysis. These findings suggest a model in which competition for divalent cations plays a pivotal role in the interaction between host and parasite...
  65. Fernandes N, Wu Q, Kong D, Puyang X, Garg S, Husson R. A mycobacterial extracytoplasmic sigma factor involved in survival following heat shock and oxidative stress. J Bacteriol. 1999;181:4266-74 pubmed
    ..Expression of the second gene in the sigH operon is required for complementation of the sigH stress phenotypes. SigH is an alternative sigma factor that plays a role in the mycobacterial stress response...
  66. Couture M, Yeh S, Wittenberg B, Wittenberg J, Ouellet Y, Rousseau D, et al. A cooperative oxygen-binding hemoglobin from Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 1999;96:11223-8 pubmed
    ..The results suggest that, physiologically, the primary role of HbN may be to protect the bacilli against reactive nitrogen species produced by the host macrophage...
  67. Ronning D, Klabunde T, Besra G, Vissa V, Belisle J, Sacchettini J. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat Struct Biol. 2000;7:141-6 pubmed publisher
    ..Also, a large region of conserved surface residues among ag85A, B and C is a probable site for the interaction of ag85 proteins with human fibronectin...
  68. Baulard A, Betts J, Engohang Ndong J, Quan S, McAdam R, Brennan P, et al. Activation of the pro-drug ethionamide is regulated in mycobacteria. J Biol Chem. 2000;275:28326-31 pubmed publisher
    ..This study opens up new avenues of research relating to ETH activation in mycobacteria, possibly leading to an improved efficacy of ETH and to the generation of new anti-mycobacterial agents...
  69. Podust L, Poulos T, Waterman M. Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors. Proc Natl Acad Sci U S A. 2001;98:3068-73 pubmed publisher
    ..These new structures provide a basis for rational design of new, more efficacious antifungal agents as well as insight into the molecular mechanism of P450 catalysis...
  70. Braunstein M, Brown A, Kurtz S, Jacobs W. Two nonredundant SecA homologues function in mycobacteria. J Bacteriol. 2001;183:6979-90 pubmed publisher
    ..This finding, in combination with the fact that SecA2 is highly conserved throughout mycobacteria, suggests a second role for SecA2. The possibility exists that another role for SecA2 is to export a specific subset of proteins...
  71. Ucko M, Colorni A, Kvitt H, Diamant A, Zlotkin A, Knibb W. Strain variation in Mycobacterium marinum fish isolates. Appl Environ Microbiol. 2002;68:5281-7 pubmed
  72. Michell S, Whelan A, Wheeler P, Panico M, Easton R, Etienne A, et al. The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1-->3)-mannobiose moieties. J Biol Chem. 2003;278:16423-32 pubmed publisher
    ..Given such a possibility characterization of mycobacterial glycoproteins is a step toward understanding their functional role and elucidating the mechanisms of mycobacterial glycosylation...
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    ..This mutant strain does not express the mycobacterial cell entry protein (Mce) and exhibits a reduced ability to invade the non-phagocytic epithelial cell line HeLa as compared to wild-type BCG...
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    ..This sequence polymorphism has been confirmed by Western blot analysis of M. bovis BCG protein extracts...
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    ..The specific reactions catalyzed by FbiA and FbiB are unknown, but both function between FO and F(420)-5,6, since FO is made by both mutants...
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    ..smegmatis, M. bovis BCG and M. tuberculosis. Therefore, InhA is the primary target of action of INH and ETH in all three species...
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    ..Identification of the genetic determinants regulated by MprA will ultimately enhance our understanding of the mechanisms utilized by M. tuberculosis to undergo latency...
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    ..Reverse transcription followed by quantitative real time PCR in response to diamide stress demonstrated that protein expression is directly proportional to the corresponding gene transcription...
  92. Gonzalo Asensio J, Maia C, Ferrer N, Barilone N, Laval F, Soto C, et al. The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in Mycobacterium tuberculosis. J Biol Chem. 2006;281:1313-6 pubmed publisher
    ..This work represents an important step toward the functional characterization of PhoP-PhoR and the understanding of complex lipid synthesis in M. tuberculosis...
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    ..tuberculosis can be achieved. Coordination of the PknB and SigH regulatory pathways through phosphorylation of RshA may lead to adaptive responses that are important in the pathogenesis of M. tuberculosis infection...
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    ..This study also provides a platform for further studies into the metabolism of M. tuberculosis using ¹³C-MFA...
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    ..We conclude that molecular identification by analysis of the 723-bp rpoB sequence is a rapid and accurate tool for identification of RGM...
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    ..At a more basic level, the characterization of the molecular and cellular function of Stealth proteins may shed light on fundamental mechanisms of innate immune defense against microbial invasion...
  98. Rawat M, Av Gay Y. Mycothiol-dependent proteins in actinomycetes. FEMS Microbiol Rev. 2007;31:278-92 pubmed publisher
    ..Following a little over a decade of research since the discovery of mycothiol in 1994, we summarize the current knowledge about the role of mycothiol as an enzyme cofactor and consider possible mycothiol-dependent enzymes...
  99. Capyk J, Kalscheuer R, Stewart G, Liu J, Kwon H, Zhao R, et al. Mycobacterial cytochrome p450 125 (cyp125) catalyzes the terminal hydroxylation of c27 steroids. J Biol Chem. 2009;284:35534-42 pubmed publisher
    ..This study establishes the catalytic function of Cyp125 and, in identifying an important difference in the catabolic potential of M. bovis and M. tuberculosis, suggests that Cyp125 may have an additional function in pathogenesis...
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    ..Altogether, these data allowed us to precisely define the functions fulfilled by the various FadD proteins encoded by the DIM + PGL cluster...
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    ..Here, the authors show that PtpA also enters the nucleus, affects the expression of several host genes, and promotes proliferation and migration of a cancer cell line. ..