Haloferax volcanii DS2


Alias: Haloferax volcanii ATCC 29605, Haloferax volcanii str. DS2, Haloferax volcanii strain DS2

Top Publications

  1. Bitan Banin G, Ortenberg R, Mevarech M. Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene. J Bacteriol. 2003;185:772-8 pubmed
    ..Application of this gene knockout system is described. ..
  2. Serrano J, Camacho M, Bonete M. Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii. FEBS Lett. 1998;434:13-6 pubmed
    ..High activities of this enzyme were detected when the carbon source was acetate. Both glyoxylate cycle key enzymes, isocitrate lyase and malate synthase, from Hf. volcanii were purified and characterized. ..
  3. Allers T, Ngo H, Mevarech M, Lloyd R. Development of additional selectable markers for the halophilic archaeon Haloferax volcanii based on the leuB and trpA genes. Appl Environ Microbiol. 2004;70:943-53 pubmed
    ..We constructed both integrative and replicative plasmids carrying the leuB or trpA gene under control of a constitutive promoter. The use of these selectable markers in deletion of the lhr gene of H. volcanii is described. ..
  4. Yurist Doutsch S, Magidovich H, Ventura V, Hitchen P, Dell A, Eichler J. N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM. Mol Microbiol. 2010;75:1047-58 pubmed publisher
    ..These experiments thus represent the first step towards in vitro reconstitution of the archaeal N-glycosylation process. ..
  5. Reuter C, Kaczowka S, Maupin Furlow J. Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii. J Bacteriol. 2004;186:7763-72 pubmed
    ..Together these results support a model in which the cell regulates the ratio of the different 20S proteasome and PAN proteins to modulate the structure and ultimately the function of this central energy-dependent proteolytic system. ..
  6. Humbard M, Zhou G, Maupin Furlow J. The N-terminal penultimate residue of 20S proteasome alpha1 influences its N(alpha) acetylation and protein levels as well as growth rate and stress responses of Haloferax volcanii. J Bacteriol. 2009;191:3794-803 pubmed publisher
  7. Delmas S, Shunburne L, Ngo H, Allers T. Mre11-Rad50 promotes rapid repair of DNA damage in the polyploid archaeon Haloferax volcanii by restraining homologous recombination. PLoS Genet. 2009;5:e1000552 pubmed publisher
    ..Our results suggest that recombination might be similarly repressed in other polyploid organisms and at repetitive sequences in haploid and diploid species. ..
  8. Humbard M, Stevens S, Maupin Furlow J. Posttranslational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii. J Bacteriol. 2006;188:7521-30 pubmed
    ..These findings represent the first evidence of acetylation and phosphorylation of archaeal proteasomes and are one of the limited examples of post- and/or cotranslational modification of proteins in this unusual group of organisms. ..
  9. Lichi T, Ring G, Eichler J. Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii. Eur J Biochem. 2004;271:1382-90 pubmed
    ..Moreover, FtsY, but not the NG domain, was shown to mediate membrane association of H. volcanii SRP54, a protein that did not otherwise interact with the membrane. ..

More Information


  1. Kaminski L, Abu Qarn M, Guan Z, Naparstek S, Ventura V, Raetz C, et al. AglJ adds the first sugar of the N-linked pentasaccharide decorating the Haloferax volcanii S-layer glycoprotein. J Bacteriol. 2010;192:5572-9 pubmed publisher
    ..Finally, while aglJ can be deleted, H. volcanii surface layer integrity is compromised in the absence of the encoded protein. ..
  2. Kaczowka S, Maupin Furlow J. Subunit topology of two 20S proteasomes from Haloferax volcanii. J Bacteriol. 2003;185:165-74 pubmed
    ..These results support a model that the ratio of alpha proteins may modulate the composition and subunit topology of 20S proteasomes in the cell. ..
  3. Miranda H, Nembhard N, Su D, Hepowit N, Krause D, Pritz J, et al. E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea. Proc Natl Acad Sci U S A. 2011;108:4417-22 pubmed publisher
    ..In sulfur transfer, SAMP1 and SAMP2 appear specific for MoCo biosynthesis and the thiolation of tRNA, respectively. Overall, this study provides a fundamental insight into the diverse cellular functions of the Ubl system. ..
  4. Wilson H, Aldrich H, Maupin Furlow J. Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis. J Bacteriol. 1999;181:5814-24 pubmed
    ..This study is the first description of a prokaryote which produces two separate 20S proteasomes and suggests that there may be distinct physiological roles for the two different alpha subunits in this halophilic archaeon. ..
  5. Guan Z, Naparstek S, Kaminski L, Konrad Z, Eichler J. Distinct glycan-charged phosphodolichol carriers are required for the assembly of the pentasaccharide N-linked to the Haloferax volcanii S-layer glycoprotein. Mol Microbiol. 2010;78:1294-303 pubmed publisher
    ..This represents the first evidence that in Archaea, as in Eukarya, the oligosaccharides N-linked to glycoproteins are sequentially assembled from glycans originating from distinct phosphodolichol carriers. ..
  6. Magidovich H, Yurist Doutsch S, Konrad Z, Ventura V, Dell A, Hitchen P, et al. AglP is a S-adenosyl-L-methionine-dependent methyltransferase that participates in the N-glycosylation pathway of Haloferax volcanii. Mol Microbiol. 2010;76:190-9 pubmed publisher
    ..volcanii S-layer glycoprotein. Subsequent purification of a tagged version of AglP and development of an in vitro assay to test the function of the protein confirmed that AglP is a S-adenosyl-L-methionine-dependent methyltransferase. ..
  7. Yurist Doutsch S, Eichler J. Manual annotation, transcriptional analysis, and protein expression studies reveal novel genes in the agl cluster responsible for N glycosylation in the halophilic archaeon Haloferax volcanii. J Bacteriol. 2009;191:3068-75 pubmed publisher
    ..Using computer-based tools or visual inspection, together with transcriptional analysis and protein expression approaches, genes encoding AglP, AglQ, and AglR are now described. ..
  8. Abu Qarn M, Yurist Doutsch S, Giordano A, Trauner A, Morris H, Hitchen P, et al. Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. J Mol Biol. 2007;374:1224-36 pubmed
    ..Thus, these results offer experimental evidence showing that N-glycosylation endows H. volcanii with an ability to maintain an intact and stable cell envelope in hypersaline surroundings, ensuring survival in this extreme environment. ..
  9. Zhao A, Gray F, MacNeill S. ATP- and NAD+-dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii. Mol Microbiol. 2006;59:743-52 pubmed
    ..Thus the LigN protein acquired by LGT appears to have been co-opted as a back-up for LigA function, perhaps to provide additional ligase activity under conditions of high genotoxic stress...
  10. Cohen Rosenzweig C, Yurist Doutsch S, Eichler J. AglS, a novel component of the Haloferax volcanii N-glycosylation pathway, is a dolichol phosphate-mannose mannosyltransferase. J Bacteriol. 2012;194:6909-16 pubmed publisher
  11. Serrano J, Bonete M. Sequencing, phylogenetic and transcriptional analysis of the glyoxylate bypass operon (ace) in the halophilic archaeon Haloferax volcanii. Biochim Biophys Acta. 2001;1520:154-62 pubmed
    ..Two sets of palindromic sequences were found in the promoter region, possibly involved in binding of transcriptional regulators (repressors and/or activators)...
  12. Woods W, Dyall Smith M. Construction and analysis of a recombination-deficient (radA) mutant of Haloferax volcanii. Mol Microbiol. 1997;23:791-7 pubmed
    ..Despite its slower growth rate, Hf. volcanii DS52 was still easy to culture and transform, and should be suitable for use in studies where a recombination-deficient background is desired...
  13. Fischer S, Maier L, Stoll B, Brendel J, Fischer E, Pfeiffer F, et al. An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA. J Biol Chem. 2012;287:33351-63 pubmed
    ..Cells could survive the plasmid challenge if their CRISPR/Cas system was altered or defective, e.g. by deletion of the cas gene cassette. Experimental PAM data were supplemented with bioinformatics data on Haloferax and Haloquadratum...
  14. Kaminski L, Guan Z, Abu Qarn M, Konrad Z, Eichler J. AglR is required for addition of the final mannose residue of the N-linked glycan decorating the Haloferax volcanii S-layer glycoprotein. Biochim Biophys Acta. 2012;1820:1664-70 pubmed publisher
    ..Although roles have been assigned to the majority of Agl proteins, others await description. In the following, the contribution of AglR to N-glycosylation was addressed...
  15. Yurist Doutsch S, Abu Qarn M, Battaglia F, Morris H, Hitchen P, Dell A, et al. AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein. Mol Microbiol. 2008;69:1234-45 pubmed publisher
    ..volcanii cells raised in different growth conditions. Such changes in N-glycosylation gene transcription levels offer additional support for the adaptive role of this post-translational modification in H. volcanii...
  16. Zhou G, Kowalczyk D, Humbard M, Rohatgi S, Maupin Furlow J. Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcanii. J Bacteriol. 2008;190:8096-105 pubmed publisher
    ..This contrasted with what was seen for panA knockouts, which displayed enhanced thermotolerance. Together, these results provide new and important insight into the biological role of proteasomes in archaea...
  17. Guan Z, Naparstek S, Calo D, Eichler J. Protein glycosylation as an adaptive response in Archaea: growth at different salt concentrations leads to alterations in Haloferax volcanii S-layer glycoprotein N-glycosylation. Environ Microbiol. 2012;14:743-53 pubmed publisher
    ..Thus, in response to changes in environmental salinity, Hfx. volcanii not only modulates the N-linked glycans decorating the S-layer glycoprotein but also the sites of such post-translational modification...
  18. Alsafadi D, Paradisi F. Covalent immobilization of alcohol dehydrogenase (ADH2) from Haloferax volcanii: how to maximize activity and optimize performance of halophilic enzymes. Mol Biotechnol. 2014;56:240-7 pubmed publisher
    ..One step purification-immobilization of this enzyme has been carried out on metal chelate-epoxy Sepabeads, as an efficient method to obtain immobilized biocatalyst directly from bacterial extracts. ..
  19. Timpson L, Liliensiek A, Alsafadi D, Cassidy J, Sharkey M, Liddell S, et al. A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii. Appl Microbiol Biotechnol. 2013;97:195-203 pubmed publisher
    ..Furthermore, HvADH2 exhibited tolerance to organic solvents. HvADH2 therefore displays much greater potential as an industrially useful biocatalyst than HvADH1. ..
  20. McDougall J, Wittmann Liebold B. Comparative analysis of the protein components from 5S rRNA.protein complexes of halophilic archaebacteria. Eur J Biochem. 1994;221:779-85 pubmed
    ..Although the L5 group of ribosomal proteins also shows a high conservation, it appears that the proteins may have had more freedom to diverge throughout evolution. ..
  21. Cerletti M, Martinez M, Gimenez M, Sastre D, Paggi R, De Castro R. The LonB protease controls membrane lipids composition and is essential for viability in the extremophilic haloarchaeon Haloferax volcanii. Environ Microbiol. 2014;16:1779-92 pubmed publisher
    ..The phenotypes associated to a membrane-bound LonB protease mutant were examined for the first time providing insight on the relevance of this protease in archaeal physiology. ..
  22. Li Y, Maciejewski M, Martin J, Jin K, Zhang Y, Maupin Furlow J, et al. Crystal structure of the ubiquitin-like small archaeal modifier protein 2 from Haloferax volcanii. Protein Sci. 2013;22:1206-17 pubmed publisher
    ..These results provide insights into the structure-function relationship of sampylating proteins of fundamental importance in post-translational protein modification and environmental cues in Archaea. ..
  23. Camacho M, Brown R, Bonete M, Danson M, Hough D. Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence. FEMS Microbiol Lett. 1995;134:85-90 pubmed
  24. Pribat A, Blaby I, Lara Núñez A, Jeanguenin L, Fouquet R, Frelin O, et al. A 5-formyltetrahydrofolate cycloligase paralog from all domains of life: comparative genomic and experimental evidence for a cryptic role in thiamin metabolism. Funct Integr Genomics. 2011;11:467-78 pubmed publisher
    ..We therefore propose (a) that COG0212 has an unrecognized yet sometimes crucial role in thiamin metabolism, most probably in salvage or detoxification, and (b) that is not a 5-FCL and should no longer be so annotated. ..
  25. McMillan L, Hepowit N, Maupin Furlow J. Archaeal Inorganic Pyrophosphatase Displays Robust Activity under High-Salt Conditions and in Organic Solvents. Appl Environ Microbiol. 2016;82:538-48 pubmed publisher
    ..Overall, we demonstrate HvPPA to be useful for hydrolyzing PPi under conditions of reduced water activity that are a hurdle to current PPA-based technologies. ..
  26. Steinert K, Kroth Pancic P, Bickel Sandkötter S. Nucleotide sequence of the ATPase A- and B-subunits of the halophilic archaebacterium Haloferax volcanii and characterization of the enzyme. Biochim Biophys Acta. 1995;1249:137-44 pubmed
    ..Bafilomycin, NBD-Cl and nitrate showed no effect. These results will be discussed in context with some specific differences in the primary structure of the Haloferax A-subunit. ..
  27. Lam W, Logan S, Doolittle W. Genes for tryptophan biosynthesis in the halophilic archaebacterium Haloferax volcanii: the trpDFEG cluster. J Bacteriol. 1992;174:1694-7 pubmed
    ..Residues involved in feedback inhibition of eubacterial anthranilate synthetases are conserved. ..
  28. Tripepi M, Esquivel R, Wirth R, Pohlschröder M. Haloferax volcanii cells lacking the flagellin FlgA2 are hypermotile. Microbiology. 2013;159:2249-58 pubmed publisher
    ..Future studies will build upon the data presented here to elucidate the significance of the hypermotility of this ?flgA2 mutant, and will illuminate the regulation and function of archaeal flagella. ..
  29. Binbuga B, Boroujerdi A, Young J. Structure in an extreme environment: NMR at high salt. Protein Sci. 2007;16:1783-7 pubmed publisher
    ..Structure calculations show that this protein has a solution structure which is similar to the previously determined crystal structure with a difference at the N terminus of beta3 and the type of beta-turn connection beta7 and beta8...
  30. Lestini R, Laptenok S, Kühn J, Hink M, Schanne Klein M, Liebl U, et al. Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication. Nucleic Acids Res. 2013;41:10358-70 pubmed publisher
    ..We suggest that the evolutionary conserved function of Hef/FANCM proteins is to enhance replication fork stability by directly interacting with collapsed replication forks. ..
  31. James K, Bonete M, Byrom D, Danson M, Hough D. Citrate synthase from Haloferax volcanii: enzyme purification and gene cloning. Biochem Soc Trans. 1992;20:12S pubmed
  32. Cerletti M, Paggi R, Guevara C, Poetsch A, De Castro R. Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii. J Proteomics. 2015;121:1-14 pubmed publisher
    ..This knowledge will advance the understanding on archaeal physiology and the biological function of membrane proteases in microorganisms. ..
  33. Ouellette M, Makkay A, Papke R. Dihydroxyacetone metabolism in Haloferax volcanii. Front Microbiol. 2013;4:376 pubmed publisher
    ..BLASTp analyses demonstrate that the DHA kinase genes are patchily distributed among the Halobacteria, whereas the glycerol kinase gene is widely distributed, suggesting a widespread capability for DHA metabolism. ..
  34. Vannice J, Skaff D, Wyckoff G, Miziorko H. Expression in Haloferax volcanii of 3-hydroxy-3-methylglutaryl coenzyme A synthase facilitates isolation and characterization of the active form of a key enzyme required for polyisoprenoid cell membrane biosynthesis in halophilic archaea. J Bacteriol. 2013;195:3854-62 pubmed publisher
    ..In in vivo experiments, hymeglusin blocks the propagation of H. volcanii cells, indicating the critical role that the mevalonate pathway plays in isoprenoid biosynthesis by these archaea. ..
  35. Maier L, Benz J, Fischer S, Alstetter M, Jaschinski K, Hilker R, et al. Deletion of the Sm1 encoding motif in the lsm gene results in distinct changes in the transcriptome and enhanced swarming activity of Haloferax cells. Biochimie. 2015;117:129-37 pubmed publisher
    ..Northern blot analyses confirmed down-regulation of two genes. In addition, the deletion strain showed a gain of function in swarming, in congruence with the up-regulation of transcripts encoding proteins required for motility. ..
  36. Gäbel K, Schmitt J, Schulz S, Näther D, Soppa J. A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants. PLoS ONE. 2013;8:e77188 pubmed publisher
    ..The phenotypes of deletion mutants and conditional depletion mutants were compared to that of the wild-type under various conditions, and growth characteristics are discussed. ..
  37. Bastard K, Perret A, Mariage A, Bessonnet T, Pinet Turpault A, Petit J, et al. Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis. Nat Chem Biol. 2017;13:858-866 pubmed publisher
    ..We also uncovered a divergent subgroup of MetX enzymes in fungi that participate only in L-cysteine biosynthesis as O-succinyl-L-serine transferases. ..
  38. Wurm J, Griese M, Bahr U, Held M, Heckel A, Karas M, et al. Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs. RNA. 2012;18:412-20 pubmed publisher
    ..However, the in-frame deletion of the pseudouridine N1-methyltransferase gene in H. volcanii did not result in a discernable phenotype in line with similar observations for knockouts of other T-arm methylating enzymes...
  39. Lam W, Cohen A, Tsouluhas D, Doolittle W. Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii. Proc Natl Acad Sci U S A. 1990;87:6614-8 pubmed
    ..Twenty-nine mutations in tryptophan biosynthesis mapped to two separate chromosomal locations. DNA sequencing of one gene cluster shows a unique gene order (trpCBA) and unusual potential secondary structures in the 5'-flanking region. ..
  40. Abdul Halim M, Pfeiffer F, Zou J, Frisch A, Haft D, Wu S, et al. Haloferax volcanii archaeosortase is required for motility, mating, and C-terminal processing of the S-layer glycoprotein. Mol Microbiol. 2013;88:1164-75 pubmed publisher
  41. Levin I, Giladi M, Altman Price N, Ortenberg R, Mevarech M. An alternative pathway for reduced folate biosynthesis in bacteria and halophilic archaea. Mol Microbiol. 2004;54:1307-18 pubmed publisher
    ..The purified recombinant H. pylori dihydropteroate synthase was found to be a flavoprotein...
  42. Mengele R, Sumper M. Drastic differences in glycosylation of related S-layer glycoproteins from moderate and extreme halophiles. J Biol Chem. 1992;267:8182-5 pubmed
    ..This is discussed in terms of a recent model explaining the stability of halophilic proteins...
  43. Vettakkorumakankav N, Danson M, Hough D, Stevenson K, Davison M, Young J. Dihydrolipoamide dehydrogenase from the halophilic archaebacterium Haloferax volcanii: characterization and N-terminal sequence. Biochem Cell Biol. 1992;70:70-5 pubmed
    ..The amino acid composition and the amino acid sequence of the first 49 residues of the N-terminus have been determined...
  44. Poidevin L, MacNeill S. Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations. BMC Mol Biol. 2006;7:44 pubmed publisher
    ..As such the LigN enzyme has potential both as a novel tool for biotechnology and as a model enzyme for studying the adaptation of proteins to high intracellular salt levels...
  45. Tozik I, Huang Q, Zwieb C, Eichler J. Reconstitution of the signal recognition particle of the halophilic archaeon Haloferax volcanii. Nucleic Acids Res. 2002;30:4166-75 pubmed
    ..volcanii SRP54 to SRP RNA is enhanced in the presence of SRP19. Finally, immunolocalization reveals that H.volcanii SRP54 is found in the cytosolic fraction, where it is associated with the ribosomal fraction of the cell...
  46. Zusman T, Rosenshine I, Boehm G, Jaenicke R, Leskiw B, Mevarech M. Dihydrofolate reductase of the extremely halophilic archaebacterium Halobacterium volcanii. The enzyme and its coding gene. J Biol Chem. 1989;264:18878-83 pubmed
    ..Preliminary biochemical characterization shows that the enzyme is unstable at salt concentrations lower than 2 M and that its activity increases with increase in the KCl or NaCl concentrations...
  47. Parente J, Casabuono A, Ferrari M, Paggi R, De Castro R, Couto A, et al. A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii. J Biol Chem. 2014;289:11304-17 pubmed publisher
    ..Furthermore, this study provides the first insight on the biological role of rhomboid proteases in Archaea, suggesting a link between protein glycosylation and this protease family...
  48. Thompson D, Palmer J, Daniels C. Expression and heat-responsive regulation of a TFIIB homologue from the archaeon Haloferax volcanii. Mol Microbiol. 1999;33:1081-92 pubmed
  49. Zafrilla B, Mart nez Espinosa R, Esclapez J, P rez Pomares F, Bonete M. SufS protein from Haloferax volcanii involved in Fe-S cluster assembly in haloarchaea. Biochim Biophys Acta. 2010;1804:1476-82 pubmed publisher
    ..In this study, we have cloned, expressed and characterised a cysteine desulphurase (SufS) from Haloferax volcanii and demonstrated that this protein is able to reconstitute the [Fe-S] cluster of halophilic ferredoxin...
  50. Kessel M, Wildhaber I, Cohen S, Baumeister W. Three-dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea. EMBO J. 1988;7:1549-54 pubmed
    ..1976) we have integrated our reconstruction into a model of halobacterial cell envelope...
  51. Bidle K, Hanson T, Howell K, Nannen J. HMG-CoA reductase is regulated by salinity at the level of transcription in Haloferax volcanii. Extremophiles. 2007;11:49-55 pubmed publisher
    ..To our knowledge, this is the first report demonstrating that the expression of HMGR is regulated in response to non-optimal salinity in a halophilic archaeon...
  52. Johnsen U, Dambeck M, Zaiss H, Fuhrer T, Soppa J, Sauer U, et al. D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii. J Biol Chem. 2009;284:27290-303 pubmed publisher
    ..However, the pathway shows similarities to proposed oxidative pentose degradation pathways to alpha-ketoglutarate in few bacteria, e.g. Azospirillum brasilense and Caulobacter crescentus, and in the archaeon Sulfolobus solfataricus...
  53. El Yacoubi B, Phillips G, Blaby I, Haas C, Cruz Y, Greenberg J, et al. A Gateway platform for functional genomics in Haloferax volcanii: deletion of three tRNA modification genes. Archaea. 2009;2:211-9 pubmed
    ..Preliminary phenotypic analysis of the deletion mutants was conducted, and confirmed all three predictions...
  54. Sherwood K, Cano D, Maupin Furlow J. Glycerol-mediated repression of glucose metabolism and glycerol kinase as the sole route of glycerol catabolism in the haloarchaeon Haloferax volcanii. J Bacteriol. 2009;191:4307-15 pubmed publisher
    ..Furthermore, our findings reveal a unique example of glycerol-induced repression of glucose metabolism in H. volcanii...
  55. Tarasov V, Pyatibratov M, Tang S, Dyall Smith M, Fedorov O. Role of flagellins from A and B loci in flagella formation of Halobacterium salinarum. Mol Microbiol. 2000;35:69-78 pubmed
    ..In broth cultures of this mutant, the medium accumulated flagella with basal body-like structures at their ends...
  56. Bracken C, Neighbor A, Lamlenn K, Thomas G, Schubert H, Whitby F, et al. Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and G. BMC Struct Biol. 2011;11:23 pubmed publisher
    ..Sequence analysis in light of the structure indicates that additional members of isoform H likely exist in the databases but have been misannotated...
  57. Charlebois R, Lam W, Cline S, Doolittle W. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium. Proc Natl Acad Sci U S A. 1987;84:8530-4 pubmed
    ..We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro...
  58. Thompson L, Daniels C. A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties. J Biol Chem. 1988;263:17951-9 pubmed
    ..The possible relationship of this enzyme to other RNA endonucleases is discussed...
  59. Sandler S, Satin L, Samra H, Clark A. recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae. Nucleic Acids Res. 1996;24:2125-32 pubmed
    ..coli and S.cerevisiae. Hence it is likely that radA in this organism is a constitutively expressed gene and we discuss possible implications of the lack of UV-inducibility...
  60. Yurist S, Dahan I, Eichler J. SRP19 is a dispensable component of the signal recognition particle in Archaea. J Bacteriol. 2007;189:276-9 pubmed publisher
    ..The absence of SRP19 did, however, increase membrane bacterioruberin levels...
  61. Watanabe M, Matsuo M, Tanaka S, Akimoto H, Asahi S, Nishimura S, et al. Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain. J Biol Chem. 1997;272:20146-51 pubmed
    ..volcanii cells. Thus, this novel TGT in H. volcanii is a key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs...
  62. Hepowit N, Uthandi S, Miranda H, Toniutti M, Prunetti L, Olivarez O, et al. Archaeal JAB1/MPN/MOV34 metalloenzyme (HvJAMM1) cleaves ubiquitin-like small archaeal modifier proteins (SAMPs) from protein-conjugates. Mol Microbiol. 2012;86:971-87 pubmed publisher
    ..HvJAMM1-type proteins are thought to release the SAMPs from proteins modified post-translationally as well as those synthesized as domain fusions...
  63. Kleman Leyer K, Armbruster D, Daniels C. Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems. Cell. 1997;89:839-47 pubmed
    ..These results provide evidence that the archaeal and eucaryal tRNA intron processing systems are related and suggest a common origin for tRNA introns in these organisms...
  64. Joshi P, Dennis P. Characterization of paralogous and orthologous members of the superoxide dismutase gene family from genera of the halophilic archaebacteria. J Bacteriol. 1993;175:1561-71 pubmed
    ..strain GRB are only about 87% identical. In the alignment of all seven sequences, there are nine codon positions where both the TCN and AGY serine codons are utilized; some or all of these may well be examples of convergent evolution...
  65. Kaminski L, Guan Z, Yurist Doutsch S, Eichler J. Two distinct N-glycosylation pathways process the Haloferax volcanii S-layer glycoprotein upon changes in environmental salinity. MBio. 2013;4:e00716-13 pubmed publisher
  66. Vannice J, Skaff D, Keightley A, Addo J, Wyckoff G, Miziorko H. Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway. J Bacteriol. 2014;196:1055-63 pubmed publisher
    ..These results also represent, to our knowledge, the first identification and characterization of any phosphomevalonate decarboxylase...
  67. Brendel J, Stoll B, Lange S, Sharma K, Lenz C, Stachler A, et al. A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (crispr)-derived rnas (crrnas) in Haloferax volcanii. J Biol Chem. 2014;289:7164-77 pubmed publisher
    ..This is the first systematic in vivo analysis of Cas6 protein variants. In addition, we show that the H. volcanii I-B system contains a Cascade-like complex with a Cas7, Cas5, and Cas6 core that protects the crRNA...
  68. Thompson L, Daniels C. Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease. J Biol Chem. 1990;265:18104-11 pubmed
  69. Lam W, Doolittle W. Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii. J Biol Chem. 1992;267:5829-34 pubmed
  70. Rawls K, Martin J, Maupin Furlow J. Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii. J Bacteriol. 2011;193:4469-76 pubmed publisher
    ..Overall, this study provides fundamental insights into glycerol metabolism in H. volcanii and enhances our understanding of central metabolic pathways of haloarchaea...
  71. Humbard M, Miranda H, Lim J, Krause D, Pritz J, Zhou G, et al. Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii. Nature. 2010;463:54-60 pubmed publisher
    ..The widespread distribution and diversity of pathways modified by SAMPylation suggest that this type of protein conjugation is central to the archaeal lineage...
  72. Phillips G, Chikwana V, Maxwell A, El Yacoubi B, Swairjo M, Iwata Reuyl D, et al. Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA. J Biol Chem. 2010;285:12706-13 pubmed publisher
    ..This is the only example of the conversion of a nitrile to a formamidine known in biology and represents a new class of amidinotransferase chemistry...
  73. Hand N, Klein R, Laskewitz A, Pohlschr der M. Archaeal and bacterial SecD and SecF homologs exhibit striking structural and functional conservation. J Bacteriol. 2006;188:1251-9 pubmed publisher
    ..Taken together, these results demonstrate significant functional conservation among the prokaryotic SecD and SecF homologs despite the distinct composition of their translocation machineries...
  74. Darwin K, Hofmann K. SAMPyling proteins in archaea. Trends Biochem Sci. 2010;35:348-51 pubmed publisher
    ..The recent discovery of ubiquitin-like proteins that form polymeric chains and covalently modify proteins in archaea finally closes the evolutionary gap among the domains of life...
  75. Kaminski L, Eichler J. Identification of residues important for the activity of Haloferax volcanii AglD, a component of the archaeal N-glycosylation pathway. Archaea. 2010;2010:315108 pubmed publisher
  76. Rawls K, Yacovone S, Maupin Furlow J. GlpR represses fructose and glucose metabolic enzymes at the level of transcription in the haloarchaeon Haloferax volcanii. J Bacteriol. 2010;192:6251-60 pubmed publisher
    ..The results presented here provide genetic and biochemical evidence that GlpR controls both fructose and glucose metabolic enzymes through transcriptional repression of the glpR-pfkB operon and kdgK1 during growth on glycerol...
  77. Irihimovitch V, Ring G, Elkayam T, Konrad Z, Eichler J. Isolation of fusion proteins containing SecY and SecE, components of the protein translocation complex from the halophilic archaeon Haloferax volcanii. Extremophiles. 2003;7:71-7 pubmed publisher
    ..Thus, CBD-cellulose interactions can be employed as a salt-insensitive affinity purification system for the capture of complexes containing the Haloferax volcanii translocation apparatus components SecY and SecE...
  78. Wanner C, Soppa J. Genetic identification of three ABC transporters as essential elements for nitrate respiration in Haloferax volcanii. Genetics. 1999;152:1417-28 pubmed
    ..The third ABC transporter is proposed to be anion specific. Genome sequencing has shown that ABC transporters are widespread in Archaea. Nevertheless, this study represents only the second example of a functional characterization...
  79. Dummer A, Bonsall J, Cihla J, Lawry S, Johnson G, Peck R. Bacterioopsin-mediated regulation of bacterioruberin biosynthesis in Halobacterium salinarum. J Bacteriol. 2011;193:5658-67 pubmed publisher
    ..Specifically, BO accumulation promotes retinal production by inhibiting bacterioruberin biosynthesis...
  80. Konrad Z, Eichler J. Lipid modification of proteins in Archaea: attachment of a mevalonic acid-based lipid moiety to the surface-layer glycoprotein of Haloferax volcanii follows protein translocation. Biochem J. 2002;366:959-64 pubmed publisher
    ..e. following protein translocation across the membrane...
  81. Rose R, Pohlschr der M. In vivo analysis of an essential archaeal signal recognition particle in its native host. J Bacteriol. 2002;184:3260-7 pubmed
  82. Naparstek S, Guan Z, Eichler J. A predicted geranylgeranyl reductase reduces the ?-position isoprene of dolichol phosphate in the halophilic archaeon, Haloferax volcanii. Biochim Biophys Acta. 2012;1821:923-33 pubmed publisher
    ..Glycosylation of dolichol phosphate in the deletion strain occurred preferentially on that version of the lipid saturated at both the ?- and ?-position isoprene subunits...
  83. Tripepi M, You J, Temel S, nder z, Brisson D, Pohlschr der M. N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella. J Bacteriol. 2012;194:4876-87 pubmed publisher
    ..volcanii flagella and demonstrate that Hfx. volcanii flagellins are excellent reporter proteins for the study of haloarchaeal glycosylation processes...
  84. Miranda H, Antelmann H, Hepowit N, Chavarria N, Krause D, Pritz J, et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. Mol Cell Proteomics. 2014;13:220-39 pubmed publisher
    ..Overall, our study of SAMP3 provides new insight into the diversity of functional ubiquitin-like protein modifiers and the network of ubiquitin-like protein targets in Archaea. ..
  85. Chatterjee K, Blaby I, Thiaville P, Majumder M, Grosjean H, Yuan Y, et al. The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA. RNA. 2012;18:421-33 pubmed publisher
    ..These results led to renaming the Archaeal COG1901 members as TrmY proteins...
  86. Sumper M, Berg E, Mengele R, Strobel I. Primary structure and glycosylation of the S-layer protein of Haloferax volcanii. J Bacteriol. 1990;172:7111-8 pubmed
    ..5-nm level attained so far (M. Kessel, I. Wildhaber, S. Cohe, and W. Baumeister, EMBO J. 7:1549-1554, 1988)...
  87. Dilks K, Gim nez M, Pohlschr der M. Genetic and biochemical analysis of the twin-arginine translocation pathway in halophilic archaea. J Bacteriol. 2005;187:8104-13 pubmed publisher
    ..Using size exclusion chromatography to further characterize cytoplasmic and membrane TatA proteins, we find these proteins present in high-molecular-weight complexes in both cellular fractions...
  88. Pickl A, Johnsen U, Sch nheit P. Fructose degradation in the haloarchaeon Haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate aldolase. J Bacteriol. 2012;194:3088-97 pubmed publisher
    ..This is the first report of the functional involvement of a bacterial-like PTS and of class II FBA in the sugar metabolism of archaea...
  89. Tripepi M, Imam S, Pohlschr der M. Haloferax volcanii flagella are required for motility but are not involved in PibD-dependent surface adhesion. J Bacteriol. 2010;192:3093-102 pubmed publisher
    ..volcanii to adhere to a glass surface, indicating that a nonflagellar type IV pilus-like structure plays a critical role in H. volcanii surface adhesion...
  90. Delmas S, Duggin I, Allers T. DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Mol Microbiol. 2013;87:168-79 pubmed publisher