Halobacterium salinarum R1


Alias: Halobacterium salinarum DSM 671, Halobacterium salinarum str. R1, Halobacterium salinarum strain R1

Top Publications

  1. Marwan W, Oesterhelt D. Quantitation of photochromism of sensory rhodopsin-I by computerized tracking of Halobacterium halobium cells. J Mol Biol. 1990;215:277-85 pubmed
    ..v. receptor to SR-I373. The kind of kinetic analysis described here, might be a useful tool in assigning spectroscopic data of pigments to photoreceptor function also in other organisms. ..
  2. Koch M, Oesterhelt D. MpcT is the transducer for membrane potential changes in Halobacterium salinarum. Mol Microbiol. 2005;55:1681-94 pubmed
    ..Htr14 was therefore renamed to Membrane potential change Transducer, or MpcT. It is the first transducer for which the causative stimulus could be narrowed to a change in DeltaPsi, as opposed to a change in pH or cellular redox state. ..
  3. Peck R, Echavarri Erasun C, Johnson E, Ng W, Kennedy S, Hood L, et al. brp and blh are required for synthesis of the retinal cofactor of bacteriorhodopsin in Halobacterium salinarum. J Biol Chem. 2001;276:5739-44 pubmed
    ..The level of beta-carotene increased approximately 5.3-fold. The simplest interpretation of these results is that brp and blh encode similar proteins that catalyze or regulate the conversion of beta-carotene to retinal. ..
  4. Tarasov V, Besir H, Schwaiger R, Klee K, Furtwängler K, Pfeiffer F, et al. A small protein from the bop-brp intergenic region of Halobacterium salinarum contains a zinc finger motif and regulates bop and crtB1 transcription. Mol Microbiol. 2008;67:772-80 pubmed publisher
    ..In silico analysis of the genomes from H. salinarum and other archaea revealed a large family of similar small zinc finger motif proteins, some of which may also be involved in transcription regulation of their adjacent genes. ..
  5. Koch M, Staudinger W, Siedler F, Oesterhelt D. Physiological sites of deamidation and methyl esterification in sensory transducers of Halobacterium salinarum. J Mol Biol. 2008;380:285-302 pubmed publisher
    ..Compared to previously reported methods, the described approach significantly facilitates the identification of physiological transducer modification sites. ..
  6. Marwan W, Bibikov S, Montrone M, Oesterhelt D. Mechanism of photosensory adaptation in Halobacterium salinarium. J Mol Biol. 1995;246:493-9 pubmed
    ..We suggest that reversible methylation of the transducer protein HtrI provides the chemical mechanism of sensory adaptation in H. salinarium and also explains the different sensitivity of the cells to orange and UV light. ..
  7. Rudolph J, Oesterhelt D. Deletion analysis of the che operon in the archaeon Halobacterium salinarium. J Mol Biol. 1996;258:548-54 pubmed
    ..These results are compared with the corresponding deletion strains in Escherichia coli and provide new insights into the eu- and archeabacterial flagellar switch. ..
  8. Schlesner M, Miller A, Streif S, Staudinger W, Müller J, Scheffer B, et al. Identification of Archaea-specific chemotaxis proteins which interact with the flagellar apparatus. BMC Microbiol. 2009;9:56 pubmed publisher
    ..Altogether, these results demonstrate that, in the archaeal domain, previously unrecognized archaea-specific Che proteins are essential for relaying taxis signaling to the flagellar apparatus. ..
  9. Sasaki J, Nara T, Spudich E, Spudich J. Constitutive activity in chimeras and deletions localize sensory rhodopsin II/HtrII signal relay to the membrane-inserted domain. Mol Microbiol. 2007;66:1321-30 pubmed

More Information


  1. Schwaiger R, Schwarz C, Furtwängler K, Tarasov V, Wende A, Oesterhelt D. Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1. BMC Mol Biol. 2010;11:40 pubmed publisher
    Archaea combine bacterial-as well as eukaryotic-like features to regulate cellular processes. Halobacterium salinarum R1 encodes eight leucine-responsive regulatory protein (Lrp)-homologues...
  2. Ferrando May E, Krah M, Marwan W, Oesterhelt D. The methyl-accepting transducer protein HtrI is functionally associated with the photoreceptor sensory rhodopsin I in the archaeon Halobacterium salinarium. EMBO J. 1993;12:2999-3005 pubmed
  3. Kokoeva M, Storch K, Klein C, Oesterhelt D. A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids. EMBO J. 2002;21:2312-22 pubmed publisher
    ..salinarum utilizes these solutes for osmotic adaptation...
  4. Rudolph J, Oesterhelt D. Chemotaxis and phototaxis require a CheA histidine kinase in the archaeon Halobacterium salinarium. EMBO J. 1995;14:667-73 pubmed
    ..This indicates that CheAH.s. plays a crucial role in chemical and light signal integration, presumably interacting with at least two phototransducers and a number of chemoreceptors...
  5. Rudolph J, Tolliday N, Schmitt C, Schuster S, Oesterhelt D. Phosphorylation in halobacterial signal transduction. EMBO J. 1995;14:4249-57 pubmed
    ..The mechanism of chemo- and phototactic signal transduction in the Archaeon H.salinarium, therefore, is similar to the two-component signaling system known from chemotaxis in the eubacterium E.coli...
  6. Betlach M, Friedman J, Boyer H, Pfeifer F. Characterization of a halobacterial gene affecting bacterio-opsin gene expression. Nucleic Acids Res. 1984;12:7949-59 pubmed
    ..The DNA sequences immediately upstream of the bop and the brp genes have significant homologies and there is a short complementary sequence. The role of the brp gene in bacterio-opsin gene expression is unclear...
  7. Grininger M, Seiler F, Zeth K, Oesterhelt D. Dodecin sequesters FAD in closed conformation from the aqueous solution. J Mol Biol. 2006;364:561-6 pubmed
    ..Moreover, in extraordinary FAD binding, dodecin serves as a model for studying bound monomeric (FAD) versus bound dimeric (e.g. riboflavin) flavin properties. ..
  8. Turner G, Chittiboyina S, Pohren L, Hines K, Correia J, Mitchell D. The bacteriorhodopsin carboxyl-terminus contributes to proton recruitment and protein stability. Biochemistry. 2009;48:1112-22 pubmed publisher
  9. Watanabe H, Ishikura T, Yamato T. Theoretical modeling of the O-intermediate structure of bacteriorhodopsin. Proteins. 2009;75:53-61 pubmed publisher
    ..The modeled structure of the O-intermediate has some implications about proton transfer in the later stages of the photocycle and the structural response of bacteriorhodopsin to the inner charge distribution. ..
  10. Rhinow D, Chizhik I, Baumann R, Noll F, Hampp N. Crystallinity of purple membranes comprising the chloride-pumping bacteriorhodopsin variant D85T and its modulation by pH and salinity. J Phys Chem B. 2010;114:15424-8 pubmed publisher
    ..Furthermore, the ability to reversibly modulate the crystallinity of PMs probably will be useful for the preparation of larger artificial crystalline arrays of BR and its variants. ..
  11. Blanck A, Oesterhelt D, Ferrando E, Schegk E, Lottspeich F. Primary structure of sensory rhodopsin I, a prokaryotic photoreceptor. EMBO J. 1989;8:3963-71 pubmed
    ..This amino acid replacement is proposed to be of crucial importance in the evolution of the slow-cycling photosensing pigment SR-I. ..
  12. Barnberg E, Hegemann P, Oesterhelt D. The chromoprotein of halorhodopsin is the light-driven electrogenic chloride pump in halobacterium halobiumt. Biochemistry. 1984;23:6216-21 pubmed
    ..The TTFB-enhanced stationary photocurrent is caused by the transport of an HA2- species. The results obtained demonstrate that the chromoprotein of halorhodopsin is the lightdriven C1- pump in H. halobiurn. ..
  13. Gulko M, Dyall Smith M, Gonzalez O, Oesterhelt D. How do haloarchaea synthesize aromatic amino acids?. PLoS ONE. 2014;9:e107475 pubmed publisher
    ..DNA microarray data indicated that the 13 genes of the canonical pathway appear to be utilised for AroAA biosynthesis in H. salinarum, as they are differentially expressed when cells are grown in medium lacking AroAA. ..
  14. Schlesner M, Miller A, Besir H, Aivaliotis M, Streif J, Scheffer B, et al. The protein interaction network of a taxis signal transduction system in a halophilic archaeon. BMC Microbiol. 2012;12:272 pubmed publisher
    ..Secondly, we propose a hypothetical feedback loop from the response regulator to Htr methylation made from the CheC proteins, CheD and CheB, which might contribute to adaptation analogous to the CheC/CheD system of B. subtilis. ..
  15. Storch K, Rudolph J, Oesterhelt D. Car: a cytoplasmic sensor responsible for arginine chemotaxis in the archaeon Halobacterium salinarum. EMBO J. 1999;18:1146-58 pubmed
    ..In both bacteria and the archaea this is the first chemoeffector molecule of a soluble methylatable transducer to be identified. ..
  16. Chuong A, Miri M, Busskamp V, Matthews G, Acker L, Sørensen A, et al. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nat Neurosci. 2014;17:1123-9 pubmed publisher
    ..The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience. ..
  17. Tamogami J, Kikukawa T, Ikeda Y, Takemura A, Demura M, Kamo N. The photochemical reaction cycle and photoinduced proton transfer of sensory rhodopsin II (Phoborhodopsin) from Halobacterium salinarum. Biophys J. 2010;98:1353-63 pubmed publisher
    ..The analysis yielded a value of 7.5 for the pKa of X-H. The proton uptake and release occurred during M-decay and O-decay, respectively. ..
  18. Bergo V, Spudich E, Spudich J, Rothschild K. Active water in protein-protein communication within the membrane: the case of SRII-HtrII signal relay. Biochemistry. 2009;48:811-3 pubmed publisher
    ..We conclude that water potentially plays an important role in the SRII --> HtrII signal transfer mechanism in the membrane's hydrophobic core. ..
  19. Morgan J, Vakkasoglu A, Lugtenburg J, Gennis R, Maeda A. Structural changes due to the deprotonation of the proton release group in the M-photointermediate of bacteriorhodopsin as revealed by time-resolved FTIR spectroscopy. Biochemistry. 2008;47:11598-605 pubmed publisher
    ..Loss of the interaction of the backbone carbonyl groups in helix G with Tyr57 and the Schiff base, and separation of Tyr57 from Arg82, may be causes of these spectral changes, leading to the stabilization of the protonated Asp85 in M. ..
  20. Wang Y, Zhao Y, Ming M, Wu J, Huang W, Ding J. Effect of substitution of proline-77 to aspartate on the light-driven proton release of bacteriorhodopsin. Photochem Photobiol. 2012;88:922-7 pubmed publisher
    ..4. The coupling strength between D85 and PRC were also weakened, as expected. These data indicate that the 77th residue in AR4 greatly account for the difference between the two proton pumps. ..
  21. Varga K, Aslimovska L, Parrot I, Dauvergne M, Haertlein M, Forsyth V, et al. NMR crystallography: the effect of deuteration on high resolution 13C solid state NMR spectra of a 7-TM protein. Biochim Biophys Acta. 2007;1768:3029-35 pubmed
    ..1) decoupling comparison of the protonated and deuterated bR imply that deuteration may be advantageous for samples in which low power 1H decoupling is required. ..
  22. Gmelin W, Zeth K, Efremov R, Heberle J, Tittor J, Oesterhelt D. The crystal structure of the L1 intermediate of halorhodopsin at 1.9 angstroms resolution. Photochem Photobiol. 2007;83:369-77 pubmed
    ..Thus, intraproteinous chloride translocation from the extracellular to the cytoplasmic part of the protein must occur in reaction steps following the L1 intermediate in the catalytic cycle of halorhodopsin. ..
  23. Kixmüller D, Greie J. An ATP-driven potassium pump promotes long-term survival of Halobacterium salinarum within salt crystals. Environ Microbiol Rep. 2012;4:234-41 pubmed publisher
    ..Therefore, a steady potassium supply, even under unfavourable energetic conditions, plays a key role in long-term survival and desiccation tolerance. ..
  24. Roychoudhury A, Bieker A, Haussinger D, Oesterhelt F. Membrane protein stability depends on the concentration of compatible solutes--a single molecule force spectroscopic study. Biol Chem. 2013;394:1465-74 pubmed publisher
    ..This also helps to understand the molecular mechanism involved in protein stabilization by organic osmolytes. ..
  25. Krebs M, RajBhandary U, Khorana H. Nucleotide sequence of ISH11, a new Halobacterium halobium insertion element isolated from the plasmid pGRB1. Nucleic Acids Res. 1990;18:6699 pubmed
  26. Dioumaev A, Lanyi J. Switch from conventional to distributed kinetics in the bacteriorhodopsin photocycle. Biochemistry. 2008;47:11125-33 pubmed publisher
  27. Leffers H, Gropp F, Lottspeich F, Zillig W, Garrett R. Sequence, organization, transcription and evolution of RNA polymerase subunit genes from the archaebacterial extreme halophiles Halobacterium halobium and Halococcus morrhuae. J Mol Biol. 1989;206:1-17 pubmed
    ..Phylogenetic analyses based on sequence alignments confirmed that the extreme halophiles belong to the archaebacterial kingdom...
  28. Spudich E, Ozorowski G, Schow E, Tobias D, Spudich J, Luecke H. A transporter converted into a sensor, a phototaxis signaling mutant of bacteriorhodopsin at 3.0 Å. J Mol Biol. 2012;415:455-63 pubmed publisher
  29. Tarasov V, Schwaiger R, Furtw ngler K, Dyall Smith M, Oesterhelt D. A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum. BMC Mol Biol. 2011;12:42 pubmed publisher
    ..The activation of the bop promoter was shown to be dependent not only on two major factors, Bat and Brz, but is also tuned by the small basic protein, Brb...
  30. Lechner J, Sumper M. The primary structure of a procaryotic glycoprotein. Cloning and sequencing of the cell surface glycoprotein gene of halobacteria. J Biol Chem. 1987;262:9724-9 pubmed
    ..14 threonine residues are clustered adjacent to this membrane anchor and linked to these threonines are all the disaccharides of the cell surface glycoprotein. 12 N-glycosylation sites are distributed over the polypeptide chain...
  31. Sineshchekov O, Sasaki J, Wang J, Spudich J. Attractant and repellent signaling conformers of sensory rhodopsin-transducer complexes. Biochemistry. 2010;49:6696-704 pubmed publisher
  32. Krah M, Marwan W, Verm glio A, Oesterhelt D. Phototaxis of Halobacterium salinarium requires a signalling complex of sensory rhodopsin I and its methyl-accepting transducer HtrI. EMBO J. 1994;13:2150-5 pubmed
    ..It is concluded that SRI and HtrI form a stable complex in the cell membrane that signals to the flagellar motor and defines absorbance maximum, photocycling rate and photochemical efficiency of SRI...
  33. Grininger M, N ll G, Traw ger S, Sinner E, Oesterhelt D. Electrochemical switching of the flavoprotein dodecin at gold surfaces modified by flavin-DNA hybrid linkers. Biointerphases. 2008;3:51-8 pubmed publisher
    ..A possible explanation for the strong influence of the surface immobilization protocol on addressing dodecin by the applied potential is that electron transfer is rather mediated by defects in the monolayer than modified ds-DNA...
  34. Peck R, Johnson E, Krebs M. Identification of a lycopene beta-cyclase required for bacteriorhodopsin biogenesis in the archaeon Halobacterium salinarum. J Bacteriol. 2002;184:2889-97 pubmed
    ..Comparative sequence analysis yields a topological model of the protein and provides a plausible evolutionary connection between heterodimeric lycopene cyclases in bacteria and bifunctional lycopene cyclase-phytoene synthases in fungi...
  35. Margolin W, Wang R, Kumar M. Isolation of an ftsZ homolog from the archaebacterium Halobacterium salinarium: implications for the evolution of FtsZ and tubulin. J Bacteriol. 1996;178:1320-7 pubmed
    ..Phylogenetic analysis demonstrated that the H. salinarium FtsZ protein is more related to tubulins than are the FtsZ proteins of eubacteria, supporting the hypothesis that FtsZ may have evolved into eukaryotic tubulin...
  36. Grininger M, Staudt H, Johansson P, Wachtveitl J, Oesterhelt D. Dodecin is the key player in flavin homeostasis of archaea. J Biol Chem. 2009;284:13068-76 pubmed publisher
    ..Intriguingly, the different structural and functional properties of a homologous bacterial dodecin suggest that dodecin has different roles in different kingdoms of life...
  37. Rudolph J, Nordmann B, Storch K, Gruenberg H, Rodewald K, Oesterhelt D. A family of halobacterial transducer proteins. FEMS Microbiol Lett. 1996;139:161-8 pubmed
    ..The Htps contain from 0 to 3 transmembrane helices and Western blotting showed that HtpIII is soluble. The arrangement of the domains in these Htps suggests a modular architecture in their construction...
  38. Strahl H, Greie J. The extremely halophilic archaeon Halobacterium salinarum R1 responds to potassium limitation by expression of the K+-transporting KdpFABC P-type ATPase and by a decrease in intracellular K+. Extremophiles. 2008;12:741-52 pubmed publisher
  39. Kokoeva M, Oesterhelt D. BasT, a membrane-bound transducer protein for amino acid detection in Halobacterium salinarum. Mol Microbiol. 2000;35:647-56 pubmed
    ..Thus, BasT and the arginine sensor Car cover the entire spectrum of chemotactic responses towards attractant amino acids in H. salinarum...
  40. Kolbe M, Besir H, Essen L, Oesterhelt D. Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution. Science. 2000;288:1390-6 pubmed
    ..Ion dragging across the protonated Schiff base explains why chloride and proton translocation modes are mechanistically equivalent in archaeal rhodopsins...
  41. Blanck A, Oesterhelt D. The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin. EMBO J. 1987;6:265-73 pubmed
    ..A ribosomal binding site is located within the translated region. The HR protein moiety is processed at the amino terminus, as well as the carboxy terminus, yielding a dominant species of calculated Mr 26 961...
  42. Bieger B, Essen L, Oesterhelt D. Crystal structure of halophilic dodecin: a novel, dodecameric flavin binding protein from Halobacterium salinarum. Structure. 2003;11:375-85 pubmed
    ..Based on the structure and the wide spread occurrences in pathogenic and soil eubacteria, a function in flavin storage or protection against radical or oxygenic stress is suggested for the dodecins...
  43. Wende A, Johansson P, Vollrath R, Dyall Smith M, Oesterhelt D, Grininger M. Structural and biochemical characterization of a halophilic archaeal alkaline phosphatase. J Mol Biol. 2010;400:52-62 pubmed publisher
    ..We compare the archaeal AP with its bacterial and eukaryotic counterparts, and we focus on the role of crown domains in enhancing protein stability, regulating enzyme function, and guiding phosphoesters into the active-site funnel...
  44. Wimmer F, Oberwinkler T, Bisle B, Tittor J, Oesterhelt D. Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum. FEBS Lett. 2008;582:3771-5 pubmed publisher
    ..This clearly demonstrates that the purified 34kD protein is the functional unit...
  45. Ruepp A, Soppa J. Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene cluster. J Bacteriol. 1996;178:4942-7 pubmed
    ..The data indicate that expression of the arc gene cluster and its regulation differ in H. salinarium and P. aeruginosa...
  46. Kixm ller D, Strahl H, Wende A, Greie J. Archaeal transcriptional regulation of the prokaryotic KdpFABC complex mediating K(+) uptake in H. salinarum. Extremophiles. 2011;15:643-52 pubmed publisher
  47. Kikuchi A, Sagami H, Ogura K. Evidence for covalent attachment of diphytanylglyceryl phosphate to the cell-surface glycoprotein of Halobacterium halobium. J Biol Chem. 1999;274:18011-6 pubmed
    ..These results indicate that the cell-surface glycoprotein (200 kDa) is modified with diphytanylglyceryl phosphate...
  48. Zhang J, Yamazaki Y, Hikake M, Murakami M, Ihara K, Kouyama T. Crystal structure of the O intermediate of the Leu93?Ala mutant of bacteriorhodopsin. Proteins. 2012;80:2384-96 pubmed publisher
    ..Another significant difference is seen in the pH dependence of the structure of the proton release group, the pK(a) value of which is suggested to be much lower in O(slow) than in M...
  49. Grininger M, Zeth K, Oesterhelt D. Dodecins: a family of lumichrome binding proteins. J Mol Biol. 2006;357:842-57 pubmed publisher
    ..Studies on mutant protein and a Halorhodospira halophila homologue spread the idea of a lumichrome binding system as a possible "waste"-trapping device, widely distributed in prokaryotes...
  50. Mescher M, Strominger J. Purification and characterization of a prokaryotic glycoprotein from the cell envelope of Halobacterium salinarium. J Biol Chem. 1976;251:2005-14 pubmed
    ..N- and O-glycosidic linkages are the most common carbohydrate-protein linkages in mammalian glycoproteins but, to our knowledge, this is the first report of either type of linkage in a prokaryotic cell envelope protein...