Chlamydomonas reinhardtii

Summary

Alias: Chlamydomonas reinhardtii strain CC-1373, Chlamydomonas reihhardtii, Chlamydomonas reinhardtii P.A.Dangeard, Chlamydomonas smithii, Chlamydomonas smithii R.W.Howshaw & H.Ettl

Top Publications

  1. Duanmu D, Miller A, Horken K, Weeks D, Spalding M. Knockdown of limiting-CO2-induced gene HLA3 decreases HCO3- transport and photosynthetic Ci affinity in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2009;106:5990-5 pubmed publisher
    The CO(2)-concentrating mechanism (CCM) of Chlamydomonas reinhardtii and other microalgal species is essential for photosynthetic growth in most natural settings...
  2. Schulz Raffelt M, Lodha M, Schroda M. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. Plant J. 2007;52:286-95 pubmed
    ..of heat shock factor 1 (HSF1), encoded by one of two HSF genes identified in the genome of Chlamydomonas reinhardtii. Chlamydomonas HSF1 shares features characteristic of class A HSFs of higher plants...
  3. Casas Mollano J, Jeong B, Xu J, Moriyama H, Cerutti H. The MUT9p kinase phosphorylates histone H3 threonine 3 and is necessary for heritable epigenetic silencing in Chlamydomonas. Proc Natl Acad Sci U S A. 2008;105:6486-91 pubmed publisher
    ..We report here that a Chlamydomonas reinhardtii mutant defective in a Ser/Thr protein kinase (MUT9p), which phosphorylates histones H3 and H2A, shows ..
  4. Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, et al. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008;7:1136-45 pubmed publisher
    ..The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein. ..
  5. Mittelmeier T, Berthold P, Danon A, Lamb M, Levitan A, Rice M, et al. C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii. Eukaryot Cell. 2008;7:2100-12 pubmed publisher
    ..and asymmetric localization of the photosensory eyespot in the biflagellate, unicellular green alga Chlamydomonas reinhardtii requires coordinated organization of photoreceptors in the plasma membrane and pigment granule/thylakoid ..
  6. Sugiyama Y, Wang H, Hikima T, Sato M, Kuroda J, Takahashi T, et al. Photocurrent attenuation by a single polar-to-nonpolar point mutation of channelrhodopsin-2. Photochem Photobiol Sci. 2009;8:328-36 pubmed publisher
    ..Our results suggest that the residue E97 is one of the molecular determinants involved in the ion flux regulation. ..
  7. van Dijk K, Marley K, Jeong B, Xu J, Hesson J, Cerny R, et al. Monomethyl histone H3 lysine 4 as an epigenetic mark for silenced euchromatin in Chlamydomonas. Plant Cell. 2005;17:2439-53 pubmed
    ..This dualistic nature of the H3K4 methyl mark has thus far remained unresolved. In the green alga Chlamydomonas reinhardtii, Mut11p, related to a subunit of trithorax-like methyltransferase complexes, is required for ..
  8. Sinetova M, Kupriyanova E, Markelova A, Allakhverdiev S, Pronina N. Identification and functional role of the carbonic anhydrase Cah3 in thylakoid membranes of pyrenoid of Chlamydomonas reinhardtii. Biochim Biophys Acta. 2012;1817:1248-55 pubmed publisher
    ..associated with thylakoid membranes in the chloroplast and pyrenoid was studied in wild-type cells of Chlamydomonas reinhardtii and in its cia3 mutant deficient in the activity of the Cah3 protein...
  9. Ma D, King Y, Kim Y, Luckett W, Boyle J, Chang Y. Amplification and characterization of an inverted repeat from the Chlamydomonas reinhardtii mitochondrial genome. Gene. 1992;119:253-7 pubmed
    ..oligos) were synthesized and used in the polymerase chain reaction (PCR) to amplify the termini of the Chlamydomonas reinhardtii mitochondrial (mt) genome. A 0...

More Information

Publications173 found, 100 shown here

  1. Casas Mollano J, van Dijk K, Eisenhart J, Cerutti H. SET3p monomethylates histone H3 on lysine 9 and is required for the silencing of tandemly repeated transgenes in Chlamydomonas. Nucleic Acids Res. 2007;35:939-50 pubmed
    ..Interestingly, a SU(VAR)3-9 homolog in the unicellular green alga Chlamydomonas reinhardtii, SET3p, functions in vitro as a specific H3K9 monomethyltransferase...
  2. Lee J, Lin H, Joo S, Goodenough U. Early sexual origins of homeoprotein heterodimerization and evolution of the plant KNOX/BELL family. Cell. 2008;133:829-40 pubmed publisher
    ..We propose that combinatorial homeoprotein-based transcriptional control, a core feature of the fungal/animal radiation, may have originated in a sexual context and enabled the evolution of land-plant body plans. ..
  3. Voytsekh O, Seitz S, Iliev D, Mittag M. Both subunits of the circadian RNA-binding protein CHLAMY1 can integrate temperature information. Plant Physiol. 2008;147:2179-93 pubmed publisher
    The circadian RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits named C1 and C3...
  4. Loiselay C, Gumpel N, Girard Bascou J, Watson A, Purton S, Wollman F, et al. Molecular identification and function of cis- and trans-acting determinants for petA transcript stability in Chlamydomonas reinhardtii chloroplasts. Mol Cell Biol. 2008;28:5529-42 pubmed publisher
    ..In the green alga Chlamydomonas reinhardtii, expression of the chloroplast petA gene, which codes for cytochrome f, depends on two specific nucleus-..
  5. Radu I, Bamann C, Nack M, Nagel G, Bamberg E, Heberle J. Conformational changes of channelrhodopsin-2. J Am Chem Soc. 2009;131:7313-9 pubmed publisher
    ..of the new class of light-gated ion channels which serve as phototaxis receptors in the green alga Chlamydomonas reinhardtii. The protein is employed in optogenetics where neural circuits are optically stimulated under high ..
  6. Ibrahim F, Rymarquis L, Kim E, Becker J, Balassa E, Green P, et al. Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas. Proc Natl Acad Sci U S A. 2010;107:3906-11 pubmed publisher
    ..Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels...
  7. Lucker B, Miller M, Dziedzic S, Blackmarr P, Cole D. Direct interactions of intraflagellar transport complex B proteins IFT88, IFT52, and IFT46. J Biol Chem. 2010;285:21508-18 pubmed publisher
    Intraflagellar transport (IFT) particles of Chlamydomonas reinhardtii contain two distinct protein complexes, A and B, composed of at least 6 and 15 protein subunits, respectively. As isolated from C...
  8. Eberhard S, Loiselay C, Drapier D, Bujaldon S, Girard Bascou J, Kuras R, et al. Dual functions of the nucleus-encoded factor TDA1 in trapping and translation activation of atpA transcripts in Chlamydomonas reinhardtii chloroplasts. Plant J. 2011;67:1055-66 pubmed publisher
    ..required for translation of the chloroplast atpA transcript that encodes subunit ? of ATP synthase in Chlamydomonas reinhardtii. The sequence of TDA1 contains eight copies of a degenerate 38-residue motif, that we named octotrico ..
  9. Strenkert D, Schmollinger S, Sommer F, Schulz Raffelt M, Schroda M. Transcription factor-dependent chromatin remodeling at heat shock and copper-responsive promoters in Chlamydomonas reinhardtii. Plant Cell. 2011;23:2285-301 pubmed publisher
    ..of the heat shock and copper starvation responses, respectively, in the unicellular green alga Chlamydomonas reinhardtii. Generally, we detected lower nucleosome occupancy, higher levels of histone H3/4 acetylation, and lower ..
  10. Houille Vernes L, Rappaport F, Wollman F, Alric J, Johnson X. Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas. Proc Natl Acad Sci U S A. 2011;108:20820-5 pubmed publisher
    ..This model suggests that, in green algae and plants, the redox poise results from the balanced accumulation of PTOX and NADPH dehydrogenase. ..
  11. Magneschi L, Catalanotti C, Subramanian V, Dubini A, Yang W, Mus F, et al. A mutant in the ADH1 gene of Chlamydomonas reinhardtii elicits metabolic restructuring during anaerobiosis. Plant Physiol. 2012;158:1293-305 pubmed publisher
    The green alga Chlamydomonas reinhardtii has numerous genes encoding enzymes that function in fermentative pathways...
  12. Esparza J, O TOOLE E, Li L, Giddings T, Kozak B, Albee A, et al. Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii. PLoS ONE. 2013;8:e53940 pubmed publisher
    ..We suggest that the triplet microtubules are likely to play a key role in katanin p80 recruitment to the basal body of Chlamydomonas rather than the transition fibers that are needed for IFT localization...
  13. Vahrenholz C, Riemen G, Pratje E, Dujon B, Michaelis G. Mitochondrial DNA of Chlamydomonas reinhardtii: the structure of the ends of the linear 15.8-kb genome suggests mechanisms for DNA replication. Curr Genet. 1993;24:241-7 pubmed
    The mitochondrial genome of Chlamydomonas reinhardtii is a linear double-stranded DNA of 15.8 kb. With the exception of the termini its DNA sequence has been published...
  14. Wirschell M, Zhao F, Yang C, Yang P, Diener D, Gaillard A, et al. Building a radial spoke: flagellar radial spoke protein 3 (RSP3) is a dimer. Cell Motil Cytoskeleton. 2008;65:238-48 pubmed
  15. Kohinata T, Nishino H, Fukuzawa H. Significance of zinc in a regulatory protein, CCM1, which regulates the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Cell Physiol. 2008;49:273-83 pubmed publisher
    ..In a unicellular green alga, Chlamydomonas reinhardtii, a regulatory factor CCM1 is indispensable for the regulation of the CCM by sensing CO(2) availability...
  16. Jans F, Mignolet E, Houyoux P, Cardol P, Ghysels B, Cuiné S, et al. A type II NAD(P)H dehydrogenase mediates light-independent plastoquinone reduction in the chloroplast of Chlamydomonas. Proc Natl Acad Sci U S A. 2008;105:20546-51 pubmed publisher
    ..approach that the NDA2 gene, belonging to a type II NAD(P)H dehydrogenases family in the green microalga Chlamydomonas reinhardtii, encodes a chloroplastic dehydrogenase that functions to reduce PQ nonphotochemically in this alga...
  17. Bower R, VanderWaal K, O TOOLE E, Fox L, Perrone C, Mueller J, et al. IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell. 2009;20:3055-63 pubmed publisher
    ..The results reveal an essential role for the IC138 subcomplex in the regulation of I1 activity by the radial spoke/phosphorylation pathway. ..
  18. Godman J, Molnar A, Baulcombe D, Balk J. RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii. Biochem J. 2010;431:345-51 pubmed publisher
    The genome of the green alga Chlamydomonas reinhardtii encodes two [FeFe]-hydrogenases, HydA1 and HydA2, and the hydrogenase-like protein HYD3...
  19. Philipps G, Krawietz D, Hemschemeier A, Happe T. A pyruvate formate lyase-deficient Chlamydomonas reinhardtii strain provides evidence for a link between fermentation and hydrogen production in green algae. Plant J. 2011;66:330-40 pubmed publisher
    The green alga Chlamydomonas reinhardtii has a complex anaerobic metabolism characterized by a plastidic hydrogenase (HYD1) coupled to photosynthesis and a bacterial-type fermentation system in which pyruvate formate lyase (PFL1) is the ..
  20. Nordhues A, Schöttler M, Unger A, Geimer S, Schönfelder S, Schmollinger S, et al. Evidence for a role of VIPP1 in the structural organization of the photosynthetic apparatus in Chlamydomonas. Plant Cell. 2012;24:637-59 pubmed publisher
    ..As this functional assignment is under debate, we investigated the function of VIPP1 in Chlamydomonas reinhardtii. Using immunofluorescence, we localized VIPP1 to distinct spots within the chloroplast...
  21. Catalanotti C, Dubini A, Subramanian V, Yang W, Magneschi L, Mus F, et al. Altered fermentative metabolism in Chlamydomonas reinhardtii mutants lacking pyruvate formate lyase and both pyruvate formate lyase and alcohol dehydrogenase. Plant Cell. 2012;24:692-707 pubmed publisher
    b>Chlamydomonas reinhardtii, a unicellular green alga, often experiences hypoxic/anoxic soil conditions that activate fermentation metabolism...
  22. Rompolas P, Patel King R, King S. Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility. Mol Biol Cell. 2012;23:3554-65 pubmed publisher
    ..These data reveal that the cell actively monitors motility and dynamically modulates flagellar levels of the dynein regulatory factor Lis1 in response to imposed alterations in beat parameters...
  23. Belzile O, Hernandez Lara C, Wang Q, Snell W. Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT. Curr Biol. 2013;23:1460-5 pubmed publisher
    ..Thus, integral membrane proteins can negotiate passage through the ciliary diffusion barrier without the need for a motor. ..
  24. Boer P, Gray M. Genes encoding a subunit of respiratory NADH dehydrogenase (ND1) and a reverse transcriptase-like protein (RTL) are linked to ribosomal RNA gene pieces in Chlamydomonas reinhardtii mitochondrial DNA. EMBO J. 1988;7:3501-8 pubmed
    ..8-kb mitochondrial genome of Chlamydomonas reinhardtii, a unicellular green alga...
  25. Boer P, Gray M. The URF 5 gene of Chlamydomonas reinhardtii mitochondria: DNA sequence and mode of transcription. EMBO J. 1986;5:21-8 pubmed
    ..mitochondrial genome has been identified in the mitochondrial DNA of the unicellular green alga, Chlamydomonas reinhardtii. The algal URF 5 gene is closely flanked by the gene for subunit I of cytochrome oxidase (COI) and by an ..
  26. Piasecki B, LaVoie M, Tam L, Lefebvre P, Silflow C. The Uni2 phosphoprotein is a cell cycle regulated component of the basal body maturation pathway in Chlamydomonas reinhardtii. Mol Biol Cell. 2008;19:262-73 pubmed
    Mutations in the UNI2 locus in Chlamydomonas reinhardtii result in a "uniflagellar" phenotype in which flagellar assembly occurs preferentially from the older basal body and ultrastructural defects reside in the transition ..
  27. Huang K, Diener D, Mitchell A, Pazour G, Witman G, Rosenbaum J. Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. J Cell Biol. 2007;179:501-14 pubmed
    ..disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned the gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with the characteristics of PKD2 family members...
  28. Berthold P, Tsunoda S, Ernst O, Mages W, Gradmann D, Hegemann P. Channelrhodopsin-1 initiates phototaxis and photophobic responses in chlamydomonas by immediate light-induced depolarization. Plant Cell. 2008;20:1665-77 pubmed publisher
    Channelrhodopsins (CHR1 and CHR2) are light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. In neuroscience, they are used to trigger action potentials by light in neuronal cells, tissues, or living ..
  29. Fischer B, Dayer R, Schwarzenbach Y, Lemaire S, Behra R, Liedtke A, et al. Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii. Plant Mol Biol. 2009;71:569-83 pubmed publisher
    ..In Chlamydomonas reinhardtii, the glutathione peroxidase homologous gene GPXH/GPX5 was shown to be specifically upregulated by ..
  30. Yamano T, Tsujikawa T, Hatano K, Ozawa S, Takahashi Y, Fukuzawa H. Light and low-CO2-dependent LCIB-LCIC complex localization in the chloroplast supports the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Cell Physiol. 2010;51:1453-68 pubmed publisher
    ..under CO2-limiting conditions in aquatic photosynthetic organisms, including the green alga Chlamydomonas reinhardtii. The CCM is assumed to be comprised of inorganic carbon transport systems that, in conjunction with ..
  31. Correa Galvis V, Redekop P, Guan K, Griess A, Truong T, Wakao S, et al. Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016;291:17478-87 pubmed publisher
    ..In the green alga Chlamydomonas reinhardtii, non-photochemical quenching becomes activated upon high light acclimation and requires the accumulation ..
  32. Thieulin Pardo G, Schramm A, Lignon S, Lebrun R, Kojadinovic M, Gontero B. The intriguing CP12-like tail of adenylate kinase 3 from Chlamydomonas reinhardtii. FEBS J. 2016;283:3389-407 pubmed publisher
    ..Here, we analyzed the chloroplast ADK 3 from the green alga, Chlamydomonas reinhardtii for the first time...
  33. Dünschede B, Träger C, Schröder C, Ziehe D, Walter B, Funke S, et al. Chloroplast SRP54 Was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution. J Biol Chem. 2015;290:13104-14 pubmed publisher
    ..This study shows that the cpSRP system in the green alga Chlamydomonas reinhardtii differs significantly from that of higher plants as cpSRP43 is not complexed to cpSRP54 in Chlamydomonas ..
  34. Huwald D, Schrapers P, Kositzki R, Haumann M, Hemschemeier A. Characterization of unusual truncated hemoglobins of Chlamydomonas reinhardtii suggests specialized functions. Planta. 2015;242:167-85 pubmed publisher
    Annotated hemoglobin genes in Chlamydomonas reinhardtii form functional globins, despite unusual architectures. Spectral characteristics show subtle biochemical differences...
  35. Marx C, Wünsch C, Kück U. The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing. Eukaryot Cell. 2015;14:998-1005 pubmed publisher
    The mRNA maturation of the tripartite chloroplast psaA gene from the green alga Chlamydomonas reinhardtii depends on various nucleus-encoded factors that participate in trans splicing of two group II introns...
  36. Grzesiuk M, Wacker A, Spijkerman E. Photosynthetic sensitivity of phytoplankton to commonly used pharmaceuticals and its dependence on cellular phosphorus status. Ecotoxicology. 2016;25:697-707 pubmed publisher
    ..b>Chlamydomonas reinhardtii was the most sensitive to fluoxetine (EC50 of 1...
  37. Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell. 2017;29:2498-2518 pubmed publisher
    The fast-growing biflagellated single-celled chlorophyte Chlamydomonas reinhardtii is the most widely used alga in basic research...
  38. Gallaher S, Fitz Gibbon S, Strenkert D, Purvine S, Pellegrini M, Merchant S. High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isol. Plant J. 2017;: pubmed publisher
    b>Chlamydomonas reinhardtii is a unicellular chlorophyte alga that is widely-studied as a reference organism for understanding photosynthesis, sensory and motile cilia, and for development of an algal-based platform for producing biofuels ..
  39. Wang J, Wang J, Liu J, Li J, Zhou L, Zhang H, et al. The evaluation of endocrine disrupting effects of tert-butylphenols towards estrogenic receptor ?, androgen receptor and thyroid hormone receptor ? and aquatic toxicities towards freshwater organisms. Environ Pollut. 2018;240:396-402 pubmed publisher
    ..with indicator organisms including Photobacterium phosphoreum, Vibrio fischeri and freshwater green alga Chlamydomonas reinhardtii. 2-TBP and 2,4-DTBP exhibited moderate antagonistic effects toward human ER? and AR in a concentration-..
  40. Costa R, Oliveira T, Ferreira J, Cardoso V, Batista F. Prospective technology on bioethanol production from photofermentation. Bioresour Technol. 2015;181:330-7 pubmed publisher
    ..This work studied the production of ethanol from green alga Chlamydomonas reinhardtii through the cellular metabolism in a light/dark cycle at 25 °C in a TAP medium with sulfur depletion...
  41. Kilaru A, Cao X, Dabbs P, Sung H, Rahman M, Thrower N, et al. Oil biosynthesis in a basal angiosperm: transcriptome analysis of Persea Americana mesocarp. BMC Plant Biol. 2015;15:203 pubmed publisher
    ..This study provides a foundation for future investigations to increase oil-content and has implications for metabolic engineering to enhance storage oil content in nonseed tissues of diverse species. ..
  42. Bajhaiya A, Dean A, Zeef L, Webster R, Pittman J. PSR1 Is a Global Transcriptional Regulator of Phosphorus Deficiency Responses and Carbon Storage Metabolism in Chlamydomonas reinhardtii. Plant Physiol. 2016;170:1216-34 pubmed publisher
    ..Here, we show that P starvation-induced lipid and starch accumulation is inhibited in a Chlamydomonas reinhardtii mutant lacking the transcription factor Pi Starvation Response1 (PSR1)...
  43. Song Z, Zhang X, Jia S, Yelick P, Zhao C. Zebrafish as a Model for Human Ciliopathies. J Genet Genomics. 2016;43:107-20 pubmed publisher
    ..Model organisms, including Chlamydomonas reinhardtii and Caenorhabditis elegans have been widely used to study the complex genetic basis of ciliopathies...
  44. Yamasaki T, Onishi M, Kim E, Cerutti H, Ohama T. RNA-binding protein DUS16 plays an essential role in primary miRNA processing in the unicellular alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016;113:10720-5 pubmed publisher
    ..an essential role in processing of primary-miRNA (pri-miRNA) transcripts in the unicellular green alga Chlamydomonas reinhardtii In animals and plants, dsRNA-binding proteins involved in miRNA biogenesis harbor two or three dsRNA-..
  45. Pandey K, Islam S, Happe T, Armstrong F. Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases. Proc Natl Acad Sci U S A. 2017;114:3843-3848 pubmed publisher
    ..Experiments were carried out on two [FeFe]-hydrogenases, CrHydA1 from the green alga Chlamydomonas reinhardtii, which contains only the active-site "H cluster," and CpI from the fermentative anaerobe ..
  46. Kronholm I, Bassett A, Baulcombe D, Collins S. Epigenetic and Genetic Contributions to Adaptation in Chlamydomonas. Mol Biol Evol. 2017;34:2285-2306 pubmed publisher
    ..We manipulated DNA methylation and histone acetylation in the unicellular green alga Chlamydomonas reinhardtii both genetically and chemically to change the amount of epigenetic variation generated or transmitted in ..
  47. Azadi Chegeni F, Schiphorst C, Pandit A. In vivo NMR as a tool for probing molecular structure and dynamics in intact Chlamydomonas reinhardtii cells. Photosynth Res. 2018;135:227-237 pubmed publisher
    ..dynamics of molecular constituents in fresh, intact cells and in freshly prepared thylakoid membranes of Chlamydomonas reinhardtii (Cr.) green algae. For isotope labeling, wild-type Cr...
  48. Sun J, Khan E, Simsek S, Ohm J, Simsek H. Bioavailability of dissolved organic nitrogen (DON) in wastewaters from animal feedlots and storage lagoons. Chemosphere. 2017;186:695-701 pubmed publisher
    ..the ABDON bioassays were comprised of individual species and several combinations involving two algae (Chlamydomonas reinhardtii and Chlorella vulgaris) and a mixed liquor suspended solids (MLSS) bacterial culture...
  49. Wang Y, Yu J, Wang P, Deng S, Chang J, Ran Z. Response of energy microalgae Chlamydomonas reinhardtii to nitrogen and phosphorus stress. Environ Sci Pollut Res Int. 2018;25:5762-5770 pubmed publisher
    ..of the N and P concentration on growth, biomass yield, protein yield, and cell ultrastructure of Chlamydomonas reinhardtii (C. reinhardtii) were investigated in this study...
  50. Korkaric M, Xiao M, Behra R, Eggen R. Acclimation of Chlamydomonas reinhardtii to ultraviolet radiation and its impact on chemical toxicity. Aquat Toxicol. 2015;167:209-19 pubmed publisher
    ..To test this for combinations of UVR and chemical stressors, we first acclimatized the model green alga Chlamydomonas reinhardtii to UVR and subsequently compared the sensitivity of UVR pre-exposed and control algae towards chemicals...
  51. Chen C, Kao A, Tsai Z, Chow T, Chang H, Zhao X, et al. Expression of type 2 diacylglycerol acyltransferse gene DGTT1 from Chlamydomonas reinhardtii enhances lipid production in Scenedesmus obliquus. Biotechnol J. 2016;11:336-44 pubmed publisher
    ..The overexpression plasmid containing the type 2 diacylglycerol acyltransferse (DGAT) gene DGTT1 from Chlamydomonas reinhardtii was constructed and transformed into S. obliquus CPC2, and the positive transformants were obtained...
  52. Wang F, Qi Y, Malnoë A, Choquet Y, Wollman F, de Vitry C. The High Light Response and Redox Control of Thylakoid FtsH Protease in Chlamydomonas reinhardtii. Mol Plant. 2017;10:99-114 pubmed publisher
    In Chlamydomonas reinhardtii, the major protease involved in the maintenance of photosynthetic machinery in thylakoid membranes, the FtsH protease, mostly forms large hetero-oligomers (?1 MDa) comprising FtsH1 and FtsH2 subunits, ..
  53. Stopka S, Mansour T, Shrestha B, Maréchal Ã, Falconet D, Vertes A. Turnover rates in microorganisms by laser ablation electrospray ionization mass spectrometry and pulse-chase analysis. Anal Chim Acta. 2016;902:1-7 pubmed publisher
    ..capabilities by determining metabolite, lipid, and peptide turnover in the photosynthetic green algae, Chlamydomonas reinhardtii, in the presence of (15)N-labeled ammonium chloride as the main nitrogen source...
  54. Duanmu D, Spalding M. Insertional suppressors of Chlamydomonas reinhardtii that restore growth of air-dier lcib mutants in low CO2. Photosynth Res. 2011;109:123-32 pubmed publisher
    b>Chlamydomonas reinhardtii and other microalgae show adaptive changes to limiting CO(2) conditions by induction of CO(2)-concentrating mechanisms...
  55. Scaife M, Smith A. Towards developing algal synthetic biology. Biochem Soc Trans. 2016;44:716-22 pubmed publisher
    ..tools and genome sequences are available for a number of model species including the green alga Chlamydomonas reinhardtii and the diatom Phaeodactylum tricornutum, although for both species there are bottlenecks to be overcome ..
  56. Tahirbegi I, Ehgartner J, Sulzer P, Zieger S, Kasjanow A, Paradiso M, et al. Fast pesticide detection inside microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence. Biosens Bioelectron. 2017;88:188-195 pubmed publisher
    ..is developed for in-situ analysis of pesticide concentration detected via metabolism/photosynthesis of Chlamydomonas reinhardtii algal cells (algae) in tap water...
  57. Qi F, Xu Y, Yu Y, Liang X, Zhang L, Zhao H, et al. Enhancing growth of Chlamydomonas reinhardtii and nutrient removal in diluted primary piggery wastewater by elevated CO2 supply. Water Sci Technol. 2017;75:2281-2290 pubmed publisher
    ..from 5% to 15% and performed best with 5% CO2 aeration in primary piggery wastewater for Chlamydomonas reinhardtii growth...
  58. Perozeni F, Stella G, Ballottari M. LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae. Int J Mol Sci. 2018;19: pubmed publisher
    ..In the model organism for green algae Chlamydomonas reinhardtii, NPQ is triggered by pigment binding proteins called light-harvesting-complexes-stress-related (..
  59. Sommer F, Drepper F, Haehnel W, Hippler M. The hydrophobic recognition site formed by residues PsaA-Trp651 and PsaB-Trp627 of photosystem I in Chlamydomonas reinhardtii confers distinct selectivity for binding of plastocyanin and cytochrome c6. J Biol Chem. 2004;279:20009-17 pubmed publisher
    ..the mutants PsaA-W651F and PsaA-W651S by site-directed mutagenesis and biolistic transformation of Chlamydomonas reinhardtii. The protein-protein interaction and the electron transfer between the donors and PSI isolated from the ..
  60. Chamizo Ampudia A, Galvan A, Fernandez E, Llamas A. Characterization of Chlamydomonas 102 and 104 mutants reveals intermolecular complementation in the molybdenum cofactor protein CNX1E. Protist. 2013;164:116-28 pubmed publisher
    b>Chlamydomonas reinhardtii is a eukaryotic, unicellular, biflagellate green alga. In Chlamydomonas nitrate assimilation depends on the activity of the enzyme nitrate reductase that requires the molybdenum cofactor (Moco)...
  61. Zaffagnini M, Michelet L, Sciabolini C, Di Giacinto N, Morisse S, Marchand C, et al. High-resolution crystal structure and redox properties of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii. Mol Plant. 2014;7:101-20 pubmed publisher
    ..Here, we have produced recombinantly and purified TPI from the unicellular green alga Chlamydomonas reinhardtii (Cr)...
  62. Heinnickel M, Kim R, Wittkopp T, Yang W, Walters K, Herbert S, et al. Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016;113:2774-9 pubmed publisher
    A Chlamydomonas reinhardtii mutant lacking CGL71, a thylakoid membrane protein previously shown to be involved in photosystem I (PSI) accumulation, exhibited photosensitivity and highly reduced abundance of PSI under photoheterotrophic ..
  63. Recuenco Muñoz L, Offre P, Valledor L, Lyon D, Weckwerth W, Wienkoop S. Targeted quantitative analysis of a diurnal RuBisCO subunit expression and translation profile in Chlamydomonas reinhardtii introducing a novel Mass Western approach. J Proteomics. 2015;113:143-53 pubmed publisher
    ..In this study the absolute amount of rbc transcripts and RSUs was quantified in Chlamydomonas reinhardtii grown during a diurnal light/dark cycle...
  64. Yang W, Catalanotti C, D Adamo S, Wittkopp T, Ingram Smith C, Mackinder L, et al. Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production. Plant Cell. 2014;26:4499-518 pubmed publisher
    b>Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1...
  65. Le Quiniou C, van Oort B, Drop B, van Stokkum I, Croce R. The High Efficiency of Photosystem I in the Green Alga Chlamydomonas reinhardtii Is Maintained after the Antenna Size Is Substantially Increased by the Association of Light-harvesting Complexes II. J Biol Chem. 2015;290:30587-95 pubmed publisher
    ..efficiency by comparing time-resolved fluorescence kinetics of PSI-LHCI and PSI-LHCI-LHCII complexes of Chlamydomonas reinhardtii. PSI-LHCI-LHCII of C...
  66. Subrahmanian N, Remacle C, Hamel P. Plant mitochondrial Complex I composition and assembly: A review. Biochim Biophys Acta. 2016;1857:1001-14 pubmed publisher
    ..I composition and assembly derived from studies in plant model systems such as Arabidopsis thaliana and Chlamydomonas reinhardtii. Plant Complex I is highly conserved and comprises a significant number of subunits also present in ..
  67. Hirashima T, Tajima N, Sato N. Draft Genome Sequences of Four Species of Chlamydomonas Containing Phosphatidylcholine. Genome Announc. 2016;4: pubmed publisher
    ..Although the model green alga Chlamydomonas reinhardtii lacks PC, four species containing PC were found in the genus Chlamydomonas Here, we report the draft ..
  68. Blaby Haas C, Merchant S. Regulating cellular trace metal economy in algae. Curr Opin Plant Biol. 2017;39:88-96 pubmed publisher
    ..focus on recent progress made toward understanding the pathways by which the single-celled, green alga Chlamydomonas reinhardtii controls its cellular trace metal economy...
  69. Jacobs J, Pudollek S, Hemschemeier A, Happe T. A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii. FEBS Lett. 2009;583:325-9 pubmed publisher
    We have found the transcript of one of at least six ferredoxin encoding genes of the green alga Chlamydomonas reinhardtii, FDX5, strongly accumulating in anaerobiosis, indicating a vital role of the encoded protein in the anaerobic ..
  70. Nohr D, Franz S, Rodriguez R, Paulus B, Essen L, Weber S, et al. Extended Electron-Transfer in Animal Cryptochromes Mediated by a Tetrad of Aromatic Amino Acids. Biophys J. 2016;111:301-311 pubmed publisher
    ..time-resolved optical and electron-paramagnetic resonance spectroscopic study of two cryptochromes from Chlamydomonas reinhardtii and Drosophila melanogaster...
  71. Larosa V, Meneghesso A, La Rocca N, Steinbeck J, Hippler M, Szabo I, et al. Mitochondria Affect Photosynthetic Electron Transport and Photosensitivity in a Green Alga. Plant Physiol. 2018;176:2305-2314 pubmed publisher
    ..This is illustrated by the observation that the strong photosensitivity of Chlamydomonas reinhardtii cells depleted of the chloroplast protein PGRL1 was rescued by the introduction of a mitochondrial ..
  72. Schulze S, Oltmanns A, Machnik N, Liu G, Xu N, Jarmatz N, et al. N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonasreinhardtii. Plant Physiol. 2018;176:1952-1964 pubmed publisher
    At present, only little is known about the enzymatic machinery required for N-glycosylation in Chlamydomonas reinhardtii, leading to the formation of N-glycans harboring Xyl and methylated Man...
  73. Batyrova K, Hallenbeck P. Hydrogen Production by a Chlamydomonas reinhardtii Strain with Inducible Expression of Photosystem II. Int J Mol Sci. 2017;18: pubmed publisher
    b>Chlamydomonas reinhardtii cy6Nac2...
  74. Tammana D, Tammana T. Chlamydomonas FAP265 is a tubulin polymerization promoting protein, essential for flagellar reassembly and hatching of daughter cells from the sporangium. PLoS ONE. 2017;12:e0185108 pubmed publisher
    ..They reassembled their flagella only after hatching from the sporangium suggesting that FAP265 plays an important role in flagellar reassembly after cell division. ..
  75. Boer P, Gray M. Nucleotide sequence of a protein coding region in Chlamydomonas reinhardtii mitochondrial DNA. Nucleic Acids Res. 1986;14:7506-7 pubmed
  76. Karkehabadi S, Satagopan S, Taylor T, Spreitzer R, Andersson I. Structural analysis of altered large-subunit loop-6/carboxy-terminus interactions that influence catalytic efficiency and CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry. 2007;46:11080-9 pubmed publisher
    ..Genetic screening in Chlamydomonas reinhardtii previously identified a loop-6 V331A substitution that decreases carboxylation and CO2/O2 specificity...
  77. Voigt J, Frank R, Wöstemeyer J. The chaotrope-soluble glycoprotein GP1 is a constituent of the insoluble glycoprotein framework of the Chlamydomonas cell wall. FEMS Microbiol Lett. 2009;291:209-15 pubmed publisher
    b>Chlamydomonas reinhardtii wild-type cells are surrounded by the insoluble cell wall component, a sac-like framework of cross-linked glycoproteins containing 22% hydroxyproline...
  78. Bruun S, Naumann H, Kuhlmann U, Schulz C, Stehfest K, Hegemann P, et al. The chromophore structure of the long-lived intermediate of the C128T channelrhodopsin-2 variant. FEBS Lett. 2011;585:3998-4001 pubmed publisher
    ..These findings imply that the parent state recovery via P380/P353 involves the transient hydrolysis and re-formation of the retinal-protein linkage. ..
  79. Ochoa Méndez C, Lara Hernández I, González L, Aguirre Banuelos P, Ibarra Barajas M, Castro Moreno P, et al. Bioactivity of an antihypertensive peptide expressed in Chlamydomonas reinhardtii. J Biotechnol. 2016;240:76-84 pubmed publisher
    ..At the same dose, the recombinant protein exerts an ACE-inhibitory effect. This is the first study that indicates the potential of this microalga producing an antihypertensive peptide as a dietary supplement for hypertension patients. ..
  80. Singh A, Sharma N, Farooqi H, Abdin M, Mock T, Kumar S. Phycoremediation of municipal wastewater by microalgae to produce biofuel. Int J Phytoremediation. 2017;19:805-812 pubmed publisher
    ..Four microalgal species (Chlamydomonas reinhardtii, Chlorella sp...
  81. Tsai C, Uygun S, Roston R, Shiu S, Benning C. Recovery from N Deprivation Is a Transcriptionally and Functionally Distinct State in Chlamydomonas. Plant Physiol. 2018;176:2007-2023 pubmed publisher
    ..from N deprivation and quiescence is an active and orderly process as we are showing here for Chlamydomonas reinhardtii We conducted comparative transcriptomics on this alga to discern processes relevant to quiescence in ..
  82. Willmund F, Dorn K, Schulz Raffelt M, Schroda M. The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 2008;148:2070-82 pubmed publisher
    ..characterization of CDJ1, one of three zinc-finger-containing J-domain proteins encoded by the Chlamydomonas reinhardtii genome. Fractionation experiments indicate that CDJ1 is a plastidic protein...
  83. Plecenikova A, Mages W, Andr sson l, Hrossova D, Valuchova S, Vlcek D, et al. Studies on recombination processes in two Chlamydomonas reinhardtii endogenous genes, NIT1 and ARG7. Protist. 2013;164:570-82 pubmed publisher
    Integration of exogenous DNA in the unicellular green alga Chlamydomonas reinhardtii is principally carried out by mechanisms involving non-homologous recombination (NHR), rather than homologous recombination (HR)...
  84. Douchi D, Qu Y, Longoni P, Legendre Lefebvre L, Johnson X, Schmitz Linneweber C, et al. A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii. Plant Cell. 2016;28:1182-99 pubmed publisher
    ..Here, we show that the maturation of psaC mutant (mac1) of Chlamydomonas reinhardtii is defective in photosystem I and fails to accumulate psaC mRNA...
  85. Paik S, Sim S, Jeon N. Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity. Bioresour Technol. 2017;233:433-437 pubmed publisher
    ..A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7...
  86. Rockwell N, Lagarias J. Ferredoxin-dependent bilin reductases in eukaryotic algae: Ubiquity and diversity. J Plant Physiol. 2017;217:57-67 pubmed publisher
    ..Bilin biosynthesis is essential for phototrophic growth in Chlamydomonas reinhardtii despite the absence of phytochromes or phycobiliproteins in this organism, raising the possibility that ..
  87. Atteia A, van Lis R, Wetterskog D, Gutiérrez Cirlos E, Ongay Larios L, Franzen L, et al. Structure, organization and expression of the genes encoding mitochondrial cytochrome c(1) and the Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Mol Genet Genomics. 2003;268:637-44 pubmed
    The sequence and organization of the Chlamydomonas reinhardtii genes encoding cytochrome c(1) ( Cyc1) and the Rieske-type iron-sulfur protein ( Isp), two key nucleus-encoded subunits of the mitochondrial cytochrome bc(1) complex, are ..
  88. Eisenhauer K, Kuhne J, Ritter E, Berndt A, Wolf S, Freier E, et al. In channelrhodopsin-2 Glu-90 is crucial for ion selectivity and is deprotonated during the photocycle. J Biol Chem. 2012;287:6904-11 pubmed publisher
    ..By dynamic homology modeling, we further hypothesized that the conductive pore is flanked by Glu-90 and located between helices A, B, C, and G. ..
  89. Formighieri C, Cazzaniga S, Kuras R, Bassi R. Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of tail-anchored proteins. Plant J. 2013;73:850-61 pubmed publisher
    as1, for antenna size mutant 1, was obtained by insertion mutagenesis of the unicellular green alga Chlamydomonas reinhardtii. This strain has a low chlorophyll content, 8% with respect to the wild type, and displays a general reduction ..
  90. Moparthi S, Thieulin Pardo G, Mansuelle P, Rigneault H, Gontero B, Wenger J. Conformational modulation and hydrodynamic radii of CP12 protein and its complexes probed by fluorescence correlation spectroscopy. FEBS J. 2014;281:3206-17 pubmed publisher
    ..Here, we characterize the diffusion dynamics and hydrodynamic radii of CP12 from Chlamydomonas reinhardtii upon binding to GAPDH and PRK using fluorescence correlation spectroscopy experiments...
  91. Hung C, Kanehara K, Nakamura Y. Isolation and characterization of a mutant defective in triacylglycerol accumulation in nitrogen-starved Chlamydomonas reinhardtii. Biochim Biophys Acta. 2016;1861:1282-1293 pubmed publisher
    Triacylglycerol (TAG), a major source of biodiesel production, accumulates in nitrogen-starved Chlamydomonas reinhardtii. However, the metabolic pathway of starch-to-TAG conversion remains elusive because an enzyme that affects the ..
  92. Nishimura T, Sato F, Ifuku K. In vivo system for analyzing the function of the PsbP protein using Chlamydomonas reinhardtii. Photosynth Res. 2017;133:117-127 pubmed publisher
    ..This study provides a platform not only for the functional analysis of PsbP in vivo but also for structural analysis of the PSII-LHCII supercomplex from green algae. ..