Yersinia pestis CO92

Summary

Alias: Yersinia pestis str. CO92, Yersinia pestis strain CO92

Top Publications

  1. Dube P, Handley S, Revell P, Miller V. The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection. Infect Immun. 2003;71:3512-20 pubmed
    ..Furthermore, these data suggest that the RovA regulon may be dispensable for Y. enterocolitica systemic disease and inflammatory responses if the Peyer's patches are bypassed. ..
  2. Dorrell N, Li S, Everest P, Dougan G, Wren B. Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant. FEMS Microbiol Lett. 1998;165:145-51 pubmed
    ..The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge. ..
  3. Pilsl H, Killmann H, Hantke K, Braun V. Periplasmic location of the pesticin immunity protein suggests inactivation of pesticin in the periplasm. J Bacteriol. 1996;178:2431-5 pubmed
    ..In the case of pesticin, recombination may have destroyed the lysis gene, of which only a rudimentary fragment exists on pPCP1. ..
  4. Petersen S, Young G. Essential role for cyclic AMP and its receptor protein in Yersinia enterocolitica virulence. Infect Immun. 2002;70:3665-72 pubmed
    ..The results indicate that the cAMP-CRP regulatory system is required for Y. enterocolitica virulence. ..
  5. Lathem W, Price P, Miller V, Goldman W. A plasminogen-activating protease specifically controls the development of primary pneumonic plague. Science. 2007;315:509-13 pubmed
    ..Inhibition of Pla expression prolonged the survival of animals with the disease, offering a therapeutic option to extend the period during which antibiotics are effective. ..
  6. Agar S, Sha J, Baze W, Erova T, Foltz S, Suarez G, et al. Deletion of Braun lipoprotein gene (lpp) and curing of plasmid pPCP1 dramatically alter the virulence of Yersinia pestis CO92 in a mouse model of pneumonic plague. Microbiology. 2009;155:3247-59 pubmed publisher
    Deletion of the murein (Braun) lipoprotein gene, lpp, attenuates the Yersinia pestis CO92 strain in mouse models of bubonic and pneumonic plague...
  7. Zhan L, Yang L, Zhou L, Li Y, Gao H, Guo Z, et al. Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis. BMC Microbiol. 2009;9:178 pubmed publisher
    ..pestis. The sycO-ypkA-yopJ promoter-proximate regions are extremely conserved in Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Therefore, data presented here can be generally applied to the above three pathogenic yersiniae. ..
  8. Plesniak L, Mahalakshmi R, Rypien C, Yang Y, Racic J, Marassi F. Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids. Biochim Biophys Acta. 2011;1808:482-9 pubmed publisher
    ..The initial NMR and CD spectra show that Ail adopts a well-defined transmembrane ?-sheet conformation in lipids...
  9. Gao H, Zhang Y, Han Y, Yang L, Liu X, Guo Z, et al. Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis. BMC Microbiol. 2011;11:39 pubmed publisher
    ..coli. The main difference is that ompF expression is not repressed at high osmolarity in Y. pestis, which is likely due to the absence of a promoter-distal OmpR-binding site for ompF. ..

More Information

Publications133 found, 100 shown here

  1. Yamashita S, Lukacik P, Barnard T, Noinaj N, Felek S, Tsang T, et al. Structural insights into Ail-mediated adhesion in Yersinia pestis. Structure. 2011;19:1672-82 pubmed publisher
    ..We also evaluated the contribution of laminin to delivery of Yops to HEp-2 cells. This work constitutes a structural description of how a bacterial outer membrane protein uses a multivalent approach to bind host cells. ..
  2. Tiner B, Sha J, Ponnusamy D, Baze W, Fitts E, Popov V, et al. Intramuscular Immunization of Mice with a Live-Attenuated Triple Mutant of Yersinia pestis CO92 Induces Robust Humoral and Cell-Mediated Immunity To Completely Protect Animals against Pneumonic Plague. Clin Vaccine Immunol. 2015;22:1255-68 pubmed publisher
    Earlier, we showed that the Δlpp ΔmsbB Δail triple mutant of Yersinia pestis CO92 with deleted genes encoding Braun lipoprotein (Lpp), an acyltransferase (MsbB), and the attachment invasion locus (Ail), respectively, was ..
  3. Moore B, New R, Butcher W, Mahood R, Steward J, Bayliss M, et al. Dual route vaccination for plague with emergency use applications. Vaccine. 2018;36:5210-5217 pubmed publisher
    ..organism challenge was achieved in Balb/c mice exposed to 2 × 104 median lethal doses of Yersinia pestis Co92, by the sub-cutaneous route at 25 days after the oral booster immunisation...
  4. Young G, Smith M, Minnich S, Miller V. The Yersinia enterocolitica motility master regulatory operon, flhDC, is required for flagellin production, swimming motility, and swarming motility. J Bacteriol. 1999;181:2823-33 pubmed
    ..Phenotypically, induction of the ptac-flhDC fusion also corresponded to increased levels of both swimming and swarming motility...
  5. Winfield M, Latifi T, Groisman E. Transcriptional regulation of the 4-amino-4-deoxy-L-arabinose biosynthetic genes in Yersinia pestis. J Biol Chem. 2005;280:14765-72 pubmed publisher
    ..Our results demonstrate that closely related bacterial species may use disparate regulatory pathways to control genes encoding conserved proteins...
  6. van Lier C, Tiner B, Chauhan S, Motin V, Fitts E, Huante M, et al. Further characterization of a highly attenuated Yersinia pestis CO92 mutant deleted for the genes encoding Braun lipoprotein and plasminogen activator protease in murine alveolar and primary human macrophages. Microb Pathog. 2015;80:27-38 pubmed publisher
    We recently characterized the Δlpp Δpla double in-frame deletion mutant of Yersinia pestis CO92 molecularly, biologically, and immunologically...
  7. Ponnusamy D, Fitts E, Sha J, Erova T, Kozlova E, Kirtley M, et al. High-throughput, signature-tagged mutagenic approach to identify novel virulence factors of Yersinia pestis CO92 in a mouse model of infection. Infect Immun. 2015;83:2065-81 pubmed publisher
  8. Cherepanov P, Mikhailova T, Karimova G, Zakharova N, Ershov I, Volkovoĭ K. [Cloning and detailed mapping of the fra-ymt region of the Yersinia pestis pFra plasmid]. Mol Gen Mikrobiol Virusol. 1991;:19-26 pubmed
    ..The antigen F1 is an 1-2 Md polymer containing a 16 kDa protein subunit. The mouse toxin a 240 kDa protein consisting of 61 kDa subunits. The nucleotide sequence of ymt gene has been defined. ..
  9. Willias S, Chauhan S, Lo C, Chain P, Motin V. CRP-Mediated Carbon Catabolite Regulation of Yersinia pestis Biofilm Formation Is Enhanced by the Carbon Storage Regulator Protein, CsrA. PLoS ONE. 2015;10:e0135481 pubmed publisher
    ..However, deletion of hmsP in the csrA-deficient mutant enabled excessive biofilm production, suggesting CsrA enables potent Y. pestis biofilm production through cyclic diguanylate regulation. ..
  10. Chen Y, Duan R, Li X, Li K, Liang J, Liu C, et al. Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis. Mol Immunol. 2015;68:290-9 pubmed publisher
    ..Immunological analysis showed the immunogenicity of each OmpA and cross-immunogenicity of OmpA for pathogenic Yersinia where OmpA may be a vaccine candidate for Y. pestis and other pathogenic Yersinia. ..
  11. Fauconnier A, Allaoui A, Campos A, Van Elsen A, Cornelis G, Bollen A. Flagellar flhA, flhB and flhE genes, organized in an operon, cluster upstream from the inv locus in Yersinia enterocolitica. Microbiology. 1997;143 ( Pt 11):3461-71 pubmed publisher
    ..Finally, the incongruous localization of inv amidst the flagellar cluster is discussed; while transposition could explain this phenomenon, no trace of such an event was detected...
  12. Iriarte M, Stainier I, Cornelis G. The rpoS gene from Yersinia enterocolitica and its influence on expression of virulence factors. Infect Immun. 1995;63:1840-7 pubmed
    ..An intact rpoS gene was necessary for full expression of yst in the stationary phase but not for the expression of myf and of pYV-encoded virulence determinants...
  13. Lillard J, Fetherston J, Pedersen L, Pendrak M, Perry R. Sequence and genetic analysis of the hemin storage (hms) system of Yersinia pestis. Gene. 1997;193:13-21 pubmed
    ..5 kDa protein with a pI of 6.68. Hms- Y. pestis strain M23-2 transformed with the cloned hmsHFRS locus developed an Hms(c) phenotype (Hms+ at 26-37 degrees C) due to mutations in genes outside the pgm locus...
  14. Derbise A, Pierre F, Merchez M, Pradel E, Laouami S, Ricard I, et al. Inheritance of the lysozyme inhibitor Ivy was an important evolutionary step by Yersinia pestis to avoid the host innate immune response. J Infect Dis. 2013;207:1535-43 pubmed publisher
    ..Our study also shows that a gene that is not necessary for the virulence of an ancestral bacterium may become essential in the emergence of a new pathogen...
  15. O Gaora P, Maskel D, Coleman D, Cafferkey M, Dougan G. Cloning and characterisation of the serC and aroA genes of Yersinia enterocolitica, and construction of an aroA mutant. Gene. 1989;84:23-30 pubmed
    ..The cloned Y. enterocolitica aroA gene was inactivated in vitro and reintroduced into the parental Y. enterocolitica 8081 strain using the suicide vector pJM703.1. Stable aroA insertion mutants of Y. enterocolitica were isolated. ..
  16. Staggs T, Perry R. Fur regulation in Yersinia species. Mol Microbiol. 1995;17:601 pubmed
  17. Heusipp G, Young G, Miller V. HreP, an in vivo-expressed protease of Yersinia enterocolitica, is a new member of the family of subtilisin/kexin-like proteases. J Bacteriol. 2001;183:3556-63 pubmed
    ..Expression of an HreP-His(6) tag fusion protein in E. coli revealed that HreP undergoes autocatalytic processing at a consensus cleavage site of subtilisin/kexin-like proteases, thereby releasing the proprotein. ..
  18. Rana R, Simpson P, Zhang M, Jennions M, Ukegbu C, Spear A, et al. Yersinia pestis TIR-domain protein forms dimers that interact with the human adaptor protein MyD88. Microb Pathog. 2011;51:89-95 pubmed publisher
    ..YpTIR is an excellent candidate for further study of the mechanism of action of pathogenic bacterial Tdps. ..
  19. Bellows L, Koestler B, Karaba S, Waters C, Lathem W. Hfq-dependent, co-ordinate control of cyclic diguanylate synthesis and catabolism in the plague pathogen Yersinia pestis. Mol Microbiol. 2012;86:661-74 pubmed publisher
    ..pestis lacking this RNA chaperone in animal models of plague...
  20. Ding Y, Fujimoto L, Yao Y, Plano G, Marassi F. Influence of the lipid membrane environment on structure and activity of the outer membrane protein Ail from Yersinia pestis. Biochim Biophys Acta. 2015;1848:712-20 pubmed publisher
    ..The data show that even when detergent micelles support the protein fold, detergents can interfere with activity in subtle ways. ..
  21. Barteneva N, Evstafieva A, Gorelov V, Wenzel B. Identification and sequencing of a plasmid (pYV96)-encoded gene product of Yersinia enterocolitica recognized by antibodies in sera of patients with autoimmune thyroid disease. Ann N Y Acad Sci. 1994;730:345-7 pubmed
  22. Nelson K, Young G, Miller V. Identification of a locus involved in systemic dissemination of Yersinia enterocolitica. Infect Immun. 2001;69:6201-8 pubmed publisher
    ..This suggests that the phenotype of an rscR mutant is due to the loss of RscA...
  23. Cantwell A, Bubeck S, Dube P. YopH inhibits early pro-inflammatory cytokine responses during plague pneumonia. BMC Immunol. 2010;11:29 pubmed publisher
    ..Altogether, our results suggest that YopH can inhibit an early pro-inflammatory response in the lungs of mice and that this is an important step in the pathogenesis of infection...
  24. Gao H, Zhang Y, Yang L, Liu X, Guo Z, Tan Y, et al. Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis. BMC Microbiol. 2011;11:40 pubmed publisher
    ..coli. Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria. ..
  25. Gaddy C, Cuevas P, Hartman L, Howe G, Worsham P, Minogue T. Development of real-time PCR assays for specific detection of hmsH, hmsF, hmsR, and irp2 located within the 102-kb pgm locus of Yersinia pestis. Mol Cell Probes. 2014;28:288-95 pubmed publisher
    ..pestis strains tested. The results from the comparative analysis document the specificity and sensitivity of the real-time PCR assays and further solidify the ostensible benefits of real-time PCR over conventional PCR. ..
  26. Osei Owusu P, Jessen Condry D, Toosky M, Roughead W, Bradley D, Nilles M. The N terminus of type III secretion needle protein YscF from Yersinia pestis functions to modulate innate immune responses. Infect Immun. 2015;83:1507-22 pubmed publisher
    ..These results suggest that the N terminus of YscF can function to decrease cytokine induction, perhaps contributing to a favorable immune environment leading to survival of Y. pestis within the eukaryotic host. ..
  27. Cao S, Liu W, Tan Y, Yang H, Zhang T, Wang T, et al. An Interaction between the Inner Rod Protein YscI and the Needle Protein YscF Is Required to Assemble the Needle Structure of the Yersinia Type Three Secretion System. J Biol Chem. 2017;292:5488-5498 pubmed publisher
    ..Consistently, YscI mutants that did not bind YscF resulted in greatly decreased HeLa cell cytotoxicity. Together, these results show that YscI participates in needle assembly by directly interacting with YscF. ..
  28. Young G, Miller V. Identification of novel chromosomal loci affecting Yersinia enterocolitica pathogenesis. Mol Microbiol. 1997;25:319-28 pubmed
    ..enterocolitica mutants were tested in the mouse model for effects on pathogenesis. All of the mutant strains were affected for virulence when assayed for survival in host tissues and LD50 analysis...
  29. Pederson K, Carlson S, Pierson D. The ClpP protein, a subunit of the Clp protease, modulates ail gene expression in Yersinia enterocolitica. Mol Microbiol. 1997;26:99-107 pubmed
    ..Thus, ClpP has a role in the modulation of ail transcription in Y. enterocolitica. ..
  30. Ellison D, Young B, Nelson K, Miller V. YmoA negatively regulates expression of invasin from Yersinia enterocolitica. J Bacteriol. 2003;185:7153-9 pubmed
    ..This mutant shows increased inv expression at 37 degrees C. The mutant can be fully complemented for inv expression by a plasmid expressing ymoA. These results indicate that YmoA plays a role in the negative regulation of inv...
  31. Jacobsen N, Bogdanovich T, Skurnik M, Lübeck P, Ahrens P, Hoorfar J. A real-time PCR assay for the specific identification of serotype O:9 of Yersinia enterocolitica. J Microbiol Methods. 2005;63:151-6 pubmed
    ..The assay was specific for the Ye O:9 tested, the detection limit was 1-10 genome copies of purified DNA and amplification efficiency was between 90.5-103%, indicating a linear regression throughout the detection window. ..
  32. Andersson J, Fitts E, Kirtley M, Ponnusamy D, Peniche A, Dann S, et al. New Role for FDA-Approved Drugs in Combating Antibiotic-Resistant Bacteria. Antimicrob Agents Chemother. 2016;60:3717-29 pubmed publisher
    ..7 murine macrophages resistant to cytotoxicity induced by the highly virulent Yersinia pestis CO92 strain...
  33. Garcia E, Nedialkov Y, Elliott J, Motin V, Brubaker R. Molecular characterization of KatY (antigen 5), a thermoregulated chromosomally encoded catalase-peroxidase of Yersinia pestis. J Bacteriol. 1999;181:3114-22 pubmed
    ..rbsA was located upstream of katY, and cybB, cybC, dmsABC, and araD were mapped downstream. These genes are not linked to katG or katP in E. coli. ..
  34. Grabenstein J, Fukuto H, Palmer L, Bliska J. Characterization of phagosome trafficking and identification of PhoP-regulated genes important for survival of Yersinia pestis in macrophages. Infect Immun. 2006;74:3727-41 pubmed publisher
    ..Viable count assays carried out with Y. pestis ugd, mgtC, and ugd mgtC mutants revealed that the products of ugd and mgtC function independently to promote early survival of Y. pestis in macrophage phagosomes...
  35. Lathem W, Schroeder J, Bellows L, Ritzert J, Koo J, Price P, et al. Posttranscriptional regulation of the Yersinia pestis cyclic AMP receptor protein Crp and impact on virulence. MBio. 2014;5:e01038-13 pubmed publisher
    ..In turn, this allows Y. pestis to fine-tune Crp levels to optimize virulence gene expression during plague infection and may allow the bacterium to adapt to its unique environmental niches. ..
  36. Eddy J, Schroeder J, Zimbler D, Caulfield A, Lathem W. Proteolysis of plasminogen activator inhibitor-1 by Yersinia pestis remodulates the host environment to promote virulence. J Thromb Haemost. 2016;14:1833-43 pubmed publisher
    ..We conclude that the inactivation of PAI-1 by Y. pestis alters the host environment to promote virulence during pneumonic plague. ..
  37. Staggs T, Perry R. Fur regulation in Yersinia species. Mol Microbiol. 1992;6:2507-16 pubmed
    ..pestis fur gene. These data indicate that Y. pestis and Y. enterocolitica possess homologous Fur systems which regulate expression of proteins in response to iron availability...
  38. Haddix P, Straley S. Structure and regulation of the Yersinia pestis yscBCDEF operon. J Bacteriol. 1992;174:4820-8 pubmed
    ..YscB and YscC were expressed in Escherichia coli by using a high-level transcription system. Attempts to express YscD were only partially successful, but they revealed interesting regulation at the translational level...
  39. Sodeinde O, Goguen J. Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of Salmonella typhimurium. Infect Immun. 1989;57:1517-23 pubmed
    ..We also identified a transcription unit that resides on the complimentary strand and overlaps the pla gene...
  40. Pepe J, Badger J, Miller V. Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene. Mol Microbiol. 1994;11:123-35 pubmed
    ..These results suggest that invasin expression in Y. enterocolitica may remain elevated early during interaction with the intestinal epithelium, a site at which invasin was shown to be necessary...
  41. Roggenkamp A, Bittner T, Leitritz L, Sing A, Heesemann J. Contribution of the Mn-cofactored superoxide dismutase (SodA) to the virulence of Yersinia enterocolitica serotype O8. Infect Immun. 1997;65:4705-10 pubmed
    ..enterocolitica could also be due to detoxification of endogenous, metabolically produced oxygen radicals which are encountered by extracellular enteric pathogens during the invasion of the host...
  42. Revell P, Miller V. A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence. Mol Microbiol. 2000;35:677-85 pubmed
    ..The more severe animal phenotype combined with the lack of impact on known virulence genes aside from inv suggests RovA regulates potentially novel virulence genes of Y. enterocolitica during infection...
  43. Oyston P, Dorrell N, Williams K, Li S, Green M, Titball R, et al. The response regulator PhoP is important for survival under conditions of macrophage-induced stress and virulence in Yersinia pestis. Infect Immun. 2000;68:3419-25 pubmed
    ..The mean lethal dose of the phoP mutant in mice was increased 75-fold in comparison with that of the wild-type strain, indicating that the PhoPQ system plays a key role in regulating the virulence of Y. pestis...
  44. Bresolin G, Morgan J, Ilgen D, Scherer S, Fuchs T. Low temperature-induced insecticidal activity of Yersinia enterocolitica. Mol Microbiol. 2006;59:503-12 pubmed publisher
    ..Overall the results suggest that the tc-PAI(Ye) could play an important role in the transmission and survival of pathogenic Y. enterocolitica strains outside mammalian hosts...
  45. Haymond A, Johny C, Dowdy T, Schweibenz B, Villarroel K, Young R, et al. Kinetic characterization and allosteric inhibition of the Yersinia pestis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (MEP synthase). PLoS ONE. 2014;9:e106243 pubmed publisher
    ..It is particularly noteworthy that allosteric regulation of MEP synthase has not been described previously. Thus, our discovery implicates an alternative site (and new chemical space) for rational drug development. ..
  46. Gupta A, Zadafiya P, Mahalakshmi R. Differential contribution of tryptophans to the folding and stability of the attachment invasion locus transmembrane β-barrel from Yersinia pestis. Sci Rep. 2014;4:6508 pubmed publisher
  47. Tiner B, Sha J, Kirtley M, Erova T, Popov V, Baze W, et al. Combinational deletion of three membrane protein-encoding genes highly attenuates yersinia pestis while retaining immunogenicity in a mouse model of pneumonic plague. Infect Immun. 2015;83:1318-38 pubmed publisher
    Previously, we showed that deletion of genes encoding Braun lipoprotein (Lpp) and MsbB attenuated Yersinia pestis CO92 in mouse and rat models of bubonic and pneumonic plague...
  48. Galyov E, Karlishev A, Volkovoy K, Denesyuk A, Nazimov I, Rubtsov K, et al. Nucleotide sequence of the Yersinia pestis gene encoding F1 antigen and the primary structure of the protein. Putative T and B cell epitopes. FEBS Lett. 1990;277:230-2 pubmed
    ..coli consensus signal sequence. The F1 was assumed to have beta-sheet structure for the most part. The region located between amino acids 100 and 150 was suggested to contain putative antigenic determinants and to stimulate T cells...
  49. Price S, Leung K, Barve S, Straley S. Molecular analysis of lcrGVH, the V antigen operon of Yersinia pestis. J Bacteriol. 1989;171:5646-53 pubmed
    ..However, two-dimensional gel analysis showed that net LcrH expression was regulated by Ca2+. We propose that lcrH lies within two differentially regulated operons, its own and lcrGVH. ..
  50. Lindler L, Tall B. Yersinia pestis pH 6 antigen forms fimbriae and is induced by intracellular association with macrophages. Mol Microbiol. 1993;8:311-24 pubmed
    ..Together with an earlier study showing that a Y. pestis psaA mutant was reduced in virulence, these results demonstrate that the expression of fimbriae which are induced in host macrophages is involved in plague pathogenesis...
  51. Yamamoto T, Miura H, Ohsumi K, Yamaguchi H, Taguchi H, Ogata S. Cloning and nucleotide sequence analysis of immunodominant heat-shock protein of Yersinia enterocolitica. Res Microbiol. 1993;144:691-701 pubmed
    ..2% identical to GroEL of Escherichia coli. CRPB, another protein encoded by the operon, shows extensive sequence homology, 91.8% identical to GroES of E. coli which is a member of chaperonin-10. ..
  52. Zhang L, Toivanen P, Skurnik M. The gene cluster directing O-antigen biosynthesis in Yersinia enterocolitica serotype 0:8: identification of the genes for mannose and galactose biosynthesis and the gene for the O-antigen polymerase. Microbiology. 1996;142 ( Pt 2):277-88 pubmed
    ..Also in other Yersinia spp., the locus downstream of the hemH gene is occupied by gene clusters associated with LPS biosynthesis. ..
  53. Chenal Francisque V, Tourneux L, Carniel E, Christova P, Li de la Sierra I, B rzu O, et al. The highly similar TMP kinases of Yersinia pestis and Escherichia coli differ markedly in their AZTMP phosphorylating activity. Eur J Biochem. 1999;265:112-9 pubmed
    ..coli enzyme suggest that segments lying outside the main regions involved in nucleotide binding and catalysis are responsible for the different rates of AZTMP phosphorylation...
  54. Bengoechea J, Skurnik M. Temperature-regulated efflux pump/potassium antiporter system mediates resistance to cationic antimicrobial peptides in Yersinia. Mol Microbiol. 2000;37:67-80 pubmed
    ..It is proposed that the RosA/RosB system protects the bacteria by both acidifying the cytoplasm to prevent the CAMPs action and pumping the CAMPs out of the cell...
  55. Swietnicki W, Powell B, Goodin J. Yersinia pestis Yop secretion protein F: purification, characterization, and protective efficacy against bubonic plague. Protein Expr Purif. 2005;42:166-72 pubmed
    ..The data were statistically significant (P = 0.053 by two-tailed Fisher's test), suggesting that the YscF protein can provide a protective immune response against lethal plague challenge during subcutaneous plague infection. ..
  56. Cornelius C, Quenee L, Elli D, Ciletti N, Schneewind O. Yersinia pestis IS1541 transposition provides for escape from plague immunity. Infect Immun. 2009;77:1807-16 pubmed publisher
    ..These findings suggest a model in which IS1541 insertion into caf1A provides for reversible changes in envelope structure, enabling Y. pestis to escape from adaptive immune responses and plague immunity...
  57. Papadopoulou E, Goodchild S, Cleary D, Weller S, Gale N, Stubberfield M, et al. Using surface-enhanced Raman spectroscopy and electrochemically driven melting to discriminate Yersinia pestis from Y. pseudotuberculosis based on single nucleotide polymorphisms within unpurified polymerase chain reaction amplicons. Anal Chem. 2015;87:1605-12 pubmed publisher
    ..Despite the variability introduced by these differences, the assays are shown to be reliable and robust providing a new platform for strain discrimination using unpurified PCR samples. ..
  58. Hare J, McDonough K. High-frequency RecA-dependent and -independent mechanisms of Congo red binding mutations in Yersinia pestis. J Bacteriol. 1999;181:4896-904 pubmed
    ..We propose that a lack of selection for the CR-binding ability of Y. pestis in vitro may contribute to the mutation frequencies observed at the hmsHFRS and hmsT loci...
  59. Jones H, Lillard J, Perry R. HmsT, a protein essential for expression of the haemin storage (Hms+) phenotype of Yersinia pestis. Microbiology. 1999;145 ( Pt 8):2117-28 pubmed publisher
    ..E. coli MG1655 contains homologues of hmsHFRST (ycdSRQPT) but has an Hms- phenotype. Only ycdQ and ycdP complement mutations in their respective homologues, hmsR and hmsS, in Y. pestis...
  60. Gelhaus H, Rozak D, Nierman W, Chen D, Varga J, Zadeh M, et al. Exogenous Yersinia pestis quorum sensing molecules N-octanoyl-homoserine lactone and N-(3-oxooctanoyl)-homoserine lactone regulate the LcrV virulence factor. Microb Pathog. 2009;46:283-7 pubmed publisher
    ..DNA microarray analysis shows 10 additional T3SS genes are consistently down-regulated by C8 or oxo-C8. This is the first report demonstrating that AHLs regulate Y. pestis virulence factor expression...
  61. Lawrenz M, Pennington J, Miller V. Acquisition of omptin reveals cryptic virulence function of autotransporter YapE in Yersinia pestis. Mol Microbiol. 2013;89:276-87 pubmed publisher
    ..pestis, was acquired along with the acquisition of pPCP1 during the divergence of Y. pestis from Y. pseudotuberculosis, and are the first evidence of a novel mechanism to regulate bacterial adherence...
  62. Karlyshev A, Galyov E, Guzayev A, Abramov V, Zav yalov V. A new gene of the f1 operon of Y. pestis involved in the capsule biogenesis. FEBS Lett. 1992;297:77-80 pubmed
    ..coli proteins involved in the biogenesis of fimbria. The deletion and in trans complementation analyses showed that this gene is not necessary for extracellular transport of the F1 subunit but plays a role in the capsule assembly...
  63. Plano G, Barve S, Straley S. LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response. J Bacteriol. 1991;173:7293-303 pubmed
    ..The alkaline phosphatase activities of the resultant hybrid proteins were consistent with a model predicting eight amino-terminal transmembrane segments that anchor a large cytoplasmic carboxyl-terminal domain to the inner membrane...
  64. Barve S, Straley S. lcrR, a low-Ca2(+)-response locus with dual Ca2(+)-dependent functions in Yersinia pestis. J Bacteriol. 1990;172:4661-71 pubmed
    ..These data suggest that the lcrR locus is necessary for the regulation of LcrG expression in the absence of Ca2+. Therefore, this locus has a dual regulatory role in the low-Ca2+ response...
  65. McDonough K, Falkow S. A Yersinia pestis-specific DNA fragment encodes temperature-dependent coagulase and fibrinolysin-associated phenotypes. Mol Microbiol. 1989;3:767-75 pubmed
    ..Our results lead us to believe that this regulation occurs post-translationally. It is possible that the alternative forms of the Pla protein are essential to 'flea blockage' and subsequent transmission of the plague bacillus to animals...
  66. Fetherston J, Lillard J, Perry R. Analysis of the pesticin receptor from Yersinia pestis: role in iron-deficient growth and possible regulation by its siderophore. J Bacteriol. 1995;177:1824-33 pubmed
    ..HMWP2 likely participates in synthesis of a siderophore which may induce expression of the receptor for pesticin and the siderophore...
  67. de Koning Ward T, Ward A, Robins Browne R. Characterisation of the urease-encoding gene complex of Yersinia enterocolitica. Gene. 1994;145:25-32 pubmed
    ..This suggests that the ure genes were acquired by Y. enterocolitica from an unrelated organism or alternatively, that they diverged from those of other Enterobacteriaceae some considerable time ago...
  68. Skurnik M, Venho R, Toivanen P, Al Hendy A. A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis. Mol Microbiol. 1995;17:575-94 pubmed
    ..enterocolitica serotypes. The results also allow us to speculate that the trs operon is a relic of the ancestral rfb region of Y. enterocolitica O:3 carrying genes indispensable for the completion of the core polysaccharide biosynthesis...
  69. Bearden S, Fetherston J, Perry R. Genetic organization of the yersiniabactin biosynthetic region and construction of avirulent mutants in Yersinia pestis. Infect Immun. 1997;65:1659-68 pubmed
    ..Finally, Y. pestis strains with mutations in either the psn or irp2 gene were avirulent in mice when inoculated subcutaneously...
  70. Goverde R, Huis in t Veld J, Kusters J, Mooi F. The psychrotrophic bacterium Yersinia enterocolitica requires expression of pnp, the gene for polynucleotide phosphorylase, for growth at low temperature (5 degrees C). Mol Microbiol. 1998;28:555-69 pubmed
    ..33 kbp region upstream of pnp harboured a cold-inducible promoter. A putative cold shock promoter motif (ATTGG) was observed in this region...
  71. Buchrieser C, Rusniok C, Frangeul L, Couve E, Billault A, Kunst F, et al. The 102-kilobase pgm locus of Yersinia pestis: sequence analysis and comparison of selected regions among different Yersinia pestis and Yersinia pseudotuberculosis strains. Infect Immun. 1999;67:4851-61 pubmed
    ..Furthermore, sequence comparisons among 29 Yersinia strains suggest that Y. pestis is a recently emerged pathogen that is probably entering the initial phase of reductive evolution...
  72. Henderson T, Nilles A, Valjavec Gratian M, Hill T. Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity. Mol Genet Genomics. 2001;265:941-53 pubmed
    ..coli to assess their ability to arrest DNA replication. The results presented here support a role for protein-protein interactions in Tus function, and suggest that residues E47 and E49 participate in replication fork arrest...
  73. Radnedge L, Agron P, Worsham P, Andersen G. Genome plasticity in Yersinia pestis. Microbiology. 2002;148:1687-98 pubmed publisher
    ..The DFR profiles detailed here demonstrate genome plasticity within Y. pestis, and they imply evolutionary relationships among the three biovars of Y. pestis, as well as between Y. pestis and Y. pseudotuberculosis...
  74. Hinnebusch B, Jarrett C, Callison J, Gardner D, Buchanan S, Plano G. Role of the Yersinia pestis Ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague. Infect Immun. 2011;79:4984-9 pubmed publisher
    ..pestis F1 antigen. The Y. pestis Ail protein is an important bubonic plague virulence factor that inhibits the innate immune response, in particular the recruitment of a protective PMN response to the infected lymph node...
  75. de la Cruz F, Carmona M, Juar z A. The Hha protein from Escherichia coli is highly homologous to the YmoA protein from Yersinia enterocolitica. Mol Microbiol. 1992;6:3451-2 pubmed
  76. Wren B, Colby S, Cubberley R, Pallen M. Degenerate PCR primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria. FEMS Microbiol Lett. 1992;78:287-91 pubmed
    ..Mutagenesis of the newly identified genes should allow us to determine their function and the genes under their control...
  77. Galyov E, Karlishev A, Chernovskaya T, Dolgikh D, Volkovoy K, Abramov V, et al. Expression of the envelope antigen F1 of Yersinia pestis is mediated by the product of caf1M gene having homology with the chaperone protein PapD of Escherichia coli. FEBS Lett. 1991;286:79-82 pubmed
    ..coli. The data presented allow one to suppose Caf1M and PapD proteins perform similar functions in the biogenesis of the Y. pestis capsule and E. coli P-pili, respectively...
  78. Cornelis G, Sluiters C, Delor I, Geib D, Kaniga K, Lambert de Rouvroit C, et al. ymoA, a Yersinia enterocolitica chromosomal gene modulating the expression of virulence functions. Mol Microbiol. 1991;5:1023-34 pubmed
    ..This work supports recent reports which propose a role for DNA topology and bacterial chromatin structure in thermoregulation of virulence functions...
  79. Badger J, Miller V. Role of RpoS in survival of Yersinia enterocolitica to a variety of environmental stresses. J Bacteriol. 1995;177:5370-3 pubmed
    ..However, a Y. enterocolitica rpoS mutant was not affected in expression of inv or ail, invasion of tissue culture cells, or virulence in mice...
  80. Iriarte M, Stainier I, Mikulskis A, Cornelis G. The fliA gene encoding sigma 28 in Yersinia enterocolitica. J Bacteriol. 1995;177:2299-304 pubmed
    ..The fliA mutant allowed us to monitor the role of motility in pathogenesis. At least in the mouse model, motility seemed not to be required for Y. enterocolitica pathogenesis...
  81. Fields K, Plano G, Straley S. A low-Ca2+ response (LCR) secretion (ysc) locus lies within the lcrB region of the LCR plasmid in Yersinia pestis. J Bacteriol. 1994;176:569-79 pubmed
  82. Guilvout I, Mercereau Puijalon O, Bonnefoy S, Pugsley A, Carniel E. High-molecular-weight protein 2 of Yersinia enterocolitica is homologous to AngR of Vibrio anguillarum and belongs to a family of proteins involved in nonribosomal peptide synthesis. J Bacteriol. 1993;175:5488-504 pubmed
    ..This finding suggests that HMWP2 may participate in the nonribosomal synthesis of small biologically active peptides...
  83. Heinrichs D, Poole K. Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa. J Bacteriol. 1993;175:5882-9 pubmed
    ..aeruginosa. In agreement with this, a PchR mutant obtained by in vitro mutagenesis and gene replacement was deficient in production of the ferripyochelin receptor and pyochelin...
  84. Skurnik M, Batsford S, Mertz A, Schiltz E, Toivanen P. The putative arthritogenic cationic 19-kilodalton antigen of Yersinia enterocolitica is a urease beta-subunit. Infect Immun. 1993;61:2498-504 pubmed
    ..Screening of a genomic library of Y. enterocolitica O:3 with a 19-kDa-antigen-specific DNA probe allowed recombinant clones containing the entire urease operon to be obtained. These clones expressed urease activity in Escherichia coli...
  85. Yamamoto T, Hanawa T, Ogata S, Kamiya S. Identification and characterization of the Yersinia enterocolitica gsrA gene, which protectively responds to intracellular stress induced by macrophage phagocytosis and to extracellular environmental stress. Infect Immun. 1996;64:2980-7 pubmed
    ..enterocolitica, allowing it to respond to the intracellular stress in macrophages as well as extracellular environmental stress...
  86. Trotot P, Sismeiro O, Vivar s C, Glaser P, Bresson Roy A, Danchin A. Comparative analysis of the cya locus in enterobacteria and related gram-negative facultative anaerobes. Biochimie. 1996;78:277-87 pubmed
    ..Finally, comparison of the different polypeptide sequences of ACs permits discussion of important features of the catalytic and regulatory centers of the protein...
  87. Zhang L, Radziejewska Lebrecht J, Krajewska Pietrasik D, Toivanen P, Skurnik M. Molecular and chemical characterization of the lipopolysaccharide O-antigen and its role in the virulence of Yersinia enterocolitica serotype O:8. Mol Microbiol. 1997;23:63-76 pubmed
    ..The virulence of the wild-type strain and that of the complemented strain were significantly higher (approx. 100-fold) than that of the rough mutant in an orally infected mouse model, showing that YeO8 O-antigen is a virulence factor...
  88. Hornung J, Jones H, Perry R. The hmu locus of Yersinia pestis is essential for utilization of free haemin and haem--protein complexes as iron sources. Mol Microbiol. 1996;20:725-39 pubmed
  89. Hare J, Wagner A, McDonough K. Independent acquisition and insertion into different chromosomal locations of the same pathogenicity island in Yersinia pestis and Yersinia pseudotuberculosis. Mol Microbiol. 1999;31:291-303 pubmed
    ..pestis and Y. pseudotuberculosis. The two YP-HPI locations and their possession of an integrase gene support a model of site-specific integration of the YP-HPI into these bacteria...
  90. Buchrieser C, Brosch R, Bach S, Guiyoule A, Carniel E. The high-pathogenicity island of Yersinia pseudotuberculosis can be inserted into any of the three chromosomal asn tRNA genes. Mol Microbiol. 1998;30:965-78 pubmed
  91. Jackson M, Plano G. DsbA is required for stable expression of outer membrane protein YscC and for efficient Yop secretion in Yersinia pestis. J Bacteriol. 1999;181:5126-30 pubmed
    ..These results suggest that YscC contains at least one disulfide bond that is essential for the function of this protein in Yop secretion...
  92. Skurnik M, Peippo A, Ervel E. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1. Mol Microbiol. 2000;37:316-30 pubmed
    ..Apparently, the expression of the O-antigen is not beneficial for the virulence or to the lifestyle of Y. pestis and, therefore, as one step in the evolution of Y. pestis, the O-antigen gene cluster was inactivated...