Vibrio cholerae O1 biovar El Tor str. N16961


Alias: Vibrio cholerae O1 biovar eltor str. N16961, Vibrio cholerae El Tor N16961, Vibrio cholerae serotype O1 biotype ElTor strain N16961, Vibrio cholerae serotype O1 biotype El Tor strain N16961

Top Publications

  1. Williams S, Varcoe L, Attridge S, Manning P. Vibrio cholerae Hcp, a secreted protein coregulated with HlyA. Infect Immun. 1996;64:283-9 pubmed
    ..We predict that an intermediate regulator may be involved in the activation of hcp by HlyU. This raises the possibility that HlyU is part of a regulatory cascade. ..
  2. Marsh J, Taylor R. Genetic and transcriptional analyses of the Vibrio cholerae mannose-sensitive hemagglutinin type 4 pilus gene locus. J Bacteriol. 1999;181:1110-7 pubmed
    ..The finding that the MSHA locus lies between these two E. coli homologs and that it is flanked by a 7-bp direct repeat suggests that the MSHA locus may have been acquired as a mobile genetic element. ..
  3. Fullner K, Mekalanos J. Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infect Immun. 1999;67:1393-404 pubmed
    ..Taken together, these data demonstrate the effectiveness of the V. cholerae genome project for rapid identification and characterization of potential virulence factors. ..
  4. Sheahan K, Cordero C, Satchell K. Identification of a domain within the multifunctional Vibrio cholerae RTX toxin that covalently cross-links actin. Proc Natl Acad Sci U S A. 2004;101:9798-803 pubmed
    ..cholerae acquired the domain by horizontal gene transfer and the ACD was inserted into the RTX toxin by gene duplication through the evolution of V. cholerae. ..
  5. Karatan E, Duncan T, Watnick P. NspS, a predicted polyamine sensor, mediates activation of Vibrio cholerae biofilm formation by norspermidine. J Bacteriol. 2005;187:7434-43 pubmed
    ..We suggest that norspermidine serves as an intercellular signaling molecule that mediates the attachment of V. cholerae to the biotic surfaces presented by one or more of these organisms. ..
  6. Häse C, Finkelstein R. Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of an HA/protease-negative strain. J Bacteriol. 1991;173:3311-7 pubmed
    ..cholerae by recombination to construct the HA/protease-negative strain HAP-1. The cloned fragment containing the hap gene was then shown to complement the mutant strain. ..
  7. Häse C, Finkelstein R. Comparison of the Vibrio cholerae hemagglutinin/protease and the Pseudomonas aeruginosa elastase. Infect Immun. 1990;58:4011-5 pubmed
    ..1 micrograms and necrosis at a higher dose (i.e., 5 micrograms). We conclude that the V. cholerae HA/protease and the P. aeruginosa elastase are structurally, functionally, and immunologically related. ..
  8. Toma C, Honma Y. Cloning and genetic analysis of the Vibrio cholerae aminopeptidase gene. Infect Immun. 1996;64:4495-500 pubmed
    ..These results suggested that the protein was a leucine aminopeptidase. The PCR analysis of lap gene distribution showed that it was widely distributed among the V. cholerae strains. It was not present in the other species examined. ..
  9. Sperandio V, Bailey C, Giron J, DiRita V, Silveira W, Vettore A, et al. Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae. Infect Immun. 1996;64:5406-9 pubmed
    ..The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains. ..

More Information

Publications158 found, 100 shown here

  1. Yildiz F, Schoolnik G. Role of rpoS in stress survival and virulence of Vibrio cholerae. J Bacteriol. 1998;180:773-84 pubmed
    ..In addition, the rpoS mutation led to reduced production or secretion of hemagglutinin/protease. However, rpoS is not critical for in vivo survival, as determined by an infant mouse intestinal competition assay. ..
  2. Lin W, Fullner K, Clayton R, Sexton J, Rogers M, Calia K, et al. Identification of a vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage. Proc Natl Acad Sci U S A. 1999;96:1071-6 pubmed
    ..Furthermore, the RTX toxin of V. cholerae may be associated with residual adverse properties displayed by certain live, attenuated cholera vaccines. ..
  3. Li C, Crawford J, DiRita V, Kaper J. Molecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae. Mol Microbiol. 2000;35:189-203 pubmed
    ..The regions protected by ToxR on each of these promoters are all A/T rich and large in size, although they are positioned differently relative to each transcriptional start site. ..
  4. Miller M, Skorupski K, Lenz D, Taylor R, Bassler B. Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell. 2002;110:303-14 pubmed
    ..cholerae. This quorum sensing apparatus is unusually complex, as it is composed of at least three parallel signaling channels. We show that in V. cholerae these communication systems converge to control virulence. ..
  5. Satchell K. MARTX, multifunctional autoprocessing repeats-in-toxin toxins. Infect Immun. 2007;75:5079-84 pubmed
  6. Pickering B, Smith D, Watnick P. Glucose-specific enzyme IIA has unique binding partners in the vibrio cholerae biofilm. MBio. 2012;3:e00228-12 pubmed publisher
    ..This work increases our understanding of the complexity of regulation by EIIA(Glc) and provides a link between the PTS and Csr networks, two global regulatory cascades that influence microbial physiology. ..
  7. Ding Y, Davis B, Waldor M. Hfq is essential for Vibrio cholerae virulence and downregulates sigma expression. Mol Microbiol. 2004;53:345-54 pubmed
    ..However, increased sigma(E) does not appear to account for this strain's reduced virulence. It is likely that sRNAs, in conjunction with Hfq, are critical regulators of V. cholerae pathogenicity. ..
  8. Mathur J, Davis B, Waldor M. Antimicrobial peptides activate the Vibrio cholerae sigmaE regulon through an OmpU-dependent signalling pathway. Mol Microbiol. 2007;63:848-58 pubmed
    ..We propose that AP-induced membrane perturbations change the conformation of OmpU to trigger a DegS-dependent sigma(E)-activating cascade. Thus, OmpU appears to act as a sensor component in a signal transduction pathway. ..
  9. Goulart C, dos Santos G, Barbosa L, Lery L, Bisch P, von Kr ger W. A ToxR-dependent role for the putative phosphoporin VCA1008 in bile salt resistance in Vibrio cholerae El Tor N16961. Microbiology. 2010;156:3011-20 pubmed publisher
  10. Huber K, Waldor M. Filamentous phage integration requires the host recombinases XerC and XerD. Nature. 2002;417:656-9 pubmed publisher
    ..cholerae chromosomes. Examination of sequences of the integration sites of other filamentous phages indicates that the XerCD recombinases also mediate the integration of these phage genomes at dif-like sites in various bacterial species...
  11. Lenz D, Mok K, Lilley B, Kulkarni R, Wingreen N, Bassler B. The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae. Cell. 2004;118:69-82 pubmed publisher
    ..We propose that Hfq, together with these sRNAs, creates an ultrasensitive regulatory switch that controls the critical transition into the high cell density, quorum-sensing mode...
  12. Das B, Bhadra R. Molecular characterization of vibrio cholerae DeltarelA DeltaspoT double mutants. Arch Microbiol. 2008;189:227-38 pubmed publisher
    ..Since these phenotypes of DeltarelA DeltaspoT mutants could be reverted back to DeltarelA phenotypes by providing SpoT in trans, it appears that the spoT gene function is crucial in V. cholerae...
  13. Ludlam A, Moore B, Xu Z. The crystal structure of ribosomal chaperone trigger factor from Vibrio cholerae. Proc Natl Acad Sci U S A. 2004;101:13436-41 pubmed publisher
    ..The dimer interface is formed between domains I and III and contains residues of mixed properties. Further implications about dimerization, ribosome binding, and other functions of trigger factor are discussed...
  14. Higgins D, Pomianek M, Kraml C, Taylor R, Semmelhack M, Bassler B. The major Vibrio cholerae autoinducer and its role in virulence factor production. Nature. 2007;450:883-6 pubmed publisher
    ..These findings suggest that CAI-1 could be used as a therapy to prevent cholera infection and, furthermore, that strategies to manipulate bacterial quorum sensing hold promise in the clinical arena...
  15. Zhang R, Westbrook M, Westbrook E, Scott D, Otwinowski Z, Maulik P, et al. The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid. J Mol Biol. 1995;251:550-62 pubmed publisher
    ..The binding of choleragenoid to the A subunit or to its receptor pentasaccharide modestly affects the local stereochemistry without perceptibly altering the subunit interface...
  16. Furuno M, Sato K, Kawagishi I, Homma M. Characterization of a flagellar sheath component, PF60, and its structural gene in marine Vibrio. J Biochem. 2000;127:29-36 pubmed
    ..The expression of the pfsA gene may be coordinately regulated with flagellar formation and similarly regulated to PF47 flagellin. ..
  17. Pickens J, Mitchell D, Liu J, Tan X, Zhang Z, Verlinde C, et al. Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer. Chem Biol. 2004;11:1205-15 pubmed publisher
  18. Lai C, Cancedda F, Chang D. Primary structure of cholera toxin subunit A1: isolation, partial sequences and alignment of the BrCN fragments. FEBS Lett. 1979;100:85-9 pubmed
  19. Nesper J, Kraiss A, Schild S, Blass J, Klose K, Bockem hl J, et al. Comparative and genetic analyses of the putative Vibrio cholerae lipopolysaccharide core oligosaccharide biosynthesis (wav) gene cluster. Infect Immun. 2002;70:2419-33 pubmed
    ..cholerae virulence...
  20. Bhowmick R, Ghosal A, Das B, Koley H, Saha D, Ganguly S, et al. Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin. Infect Immun. 2008;76:4968-77 pubmed publisher
  21. Kovacikova G, Skorupski K. A Vibrio cholerae LysR homolog, AphB, cooperates with AphA at the tcpPH promoter to activate expression of the ToxR virulence cascade. J Bacteriol. 1999;181:4250-6 pubmed
    ..These results suggest that AphB plays a role in the differential regulation of virulence genes between the two disease-causing biotypes...
  22. Abendroth J, Bagdasarian M, Sandkvist M, Hol W. The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily. J Mol Biol. 2004;344:619-33 pubmed publisher
    ..Functional data as well as structural homology and sequence conservation suggest that domain II interacts with EpsE, the major cytoplasmic binding partner of EpsL...
  23. Childers B, Weber G, Prouty M, Castaneda M, Peng F, Klose K. Identification of residues critical for the function of the Vibrio cholerae virulence regulator ToxT by scanning alanine mutagenesis. J Mol Biol. 2007;367:1413-30 pubmed publisher
    ..This study illuminates the contribution of specific amino acids to the dimerization, DNA binding, and transcriptional activity of ToxT...
  24. Gebhart C, Ielmini M, Reiz B, Price N, Aas F, Koomey M, et al. Characterization of exogenous bacterial oligosaccharyltransferases in Escherichia coli reveals the potential for O-linked protein glycosylation in Vibrio cholerae and Burkholderia thailandensis. Glycobiology. 2012;22:962-74 pubmed publisher
  25. Klapper D, Finkelstein R, Capra J. Subunit structure and N-terminal amino acid sequence of the three chains of cholera enterotoxin. Immunochemistry. 1976;13:605-11 pubmed
  26. Trucksis M, Galen J, Michalski J, Fasano A, Kaper J. Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette. Proc Natl Acad Sci U S A. 1993;90:5267-71 pubmed
    ..The gene encoding Ace is located immediately upstream of the genes encoding Zot and cholera toxin. The ctx, zot, and ace genes, which are located on a dynamic sector of the chromosome, comprise a V. cholerae "virulence cassette."..
  27. Hiratsu K, Yamamoto K, Makino K. Molecular cloning and functional analysis of an rpoS homologue gene from Vibrio cholerae N86. Genes Genet Syst. 1997;72:115-8 pubmed
    ..coli. In addition, the nlpD-like gene was found in the upstream region of the rpoS gene in the same order as in E. coli. These results suggest that the organization of these genes is highly conserved between E. coli and V. cholerae. ..
  28. Bik E, Gouw R, Mooi F. DNA fingerprinting of Vibrio cholerae strains with a novel insertion sequence element: a tool to identify epidemic strains. J Clin Microbiol. 1996;34:1453-61 pubmed
    ..cholerae strain carrying non-O1 antigens. This study underlines the importance of genotypic methods for the differentiation of V. cholerae strains and for recognition of strains with epidemic potential...
  29. Pal R, Bag S, Dasgupta S, Das B, Bhadra R. Functional characterization of the stringent response regulatory gene dksA of Vibrio cholerae and its role in modulation of virulence phenotypes. J Bacteriol. 2012;194:5638-48 pubmed publisher
    ..cholerae. Altogether, it appears that apart from stringent-response regulation, DksA(Vc) also has important roles in fine regulation of virulence-related phenotypes of V. cholerae...
  30. Lai C. Determination of the primary structure of cholera toxin B subunit. J Biol Chem. 1977;252:7249-56 pubmed
    ..The chymotryptic peptides from the citraconylated B subunit proved to be useful in the alignment of the tryptic peptide. ..
  31. Klose K, Novik V, Mekalanos J. Identification of multiple sigma54-dependent transcriptional activators in Vibrio cholerae. J Bacteriol. 1998;180:5256-9 pubmed
    ..None of the mutant strains exhibited a defect in the ability to colonize infant mice, suggesting the presence of additional sigma54-dependent activators not identified by this technique. ..
  32. Duvanova O, Mishankin B, Vodopianov A, Sorokin V. [N-ACETYL-?-D-GLUCOSAMINIDASE OF VIBRIO CHOLERAE]. Zh Mikrobiol Epidemiol Immunobiol. 2016;:41-8 pubmed
  33. Baudry B, Fasano A, Ketley J, Kaper J. Cloning of a gene (zot) encoding a new toxin produced by Vibrio cholerae. Infect Immun. 1992;60:428-34 pubmed
    ..3-kb open reading frame which could potentially encode a 44.8-kDa polypeptide. The location of the zot gene encoding the new toxin is immediately upstream of the ctx operon encoding cholera toxin...
  34. Davis B, Kimsey H, Chang W, Waldor M. The Vibrio cholerae O139 Calcutta bacteriophage CTXphi is infectious and encodes a novel repressor. J Bacteriol. 1999;181:6779-87 pubmed
    ..Analysis of the prophage arrays generated by sequentially integrated CTX phages revealed that pathogenic V. cholerae O139 Calcutta probably arose via infection of an O139 CTX(ET)phi lysogen by CTX(calc)phi...
  35. Krumholz L, Esser U, Simoni R. Nucleotide sequence of the unc operon of Vibrio alginolyticus. Nucleic Acids Res. 1989;17:7993-4 pubmed
  36. Butterton J, Choi M, Watnick P, Carroll P, Calderwood S. Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases. J Bacteriol. 2000;182:1731-8 pubmed
    ..The protein product of vibF is homologous to the multifunctional nonribosomal protein synthetases and is necessary for the biosynthesis of vibriobactin. ..
  37. Nusrin S, Khan G, Bhuiyan N, Ansaruzzaman M, Hossain M, Safa A, et al. Diverse CTX phages among toxigenic Vibrio cholerae O1 and O139 strains isolated between 1994 and 2002 in an area where cholera is endemic in Bangladesh. J Clin Microbiol. 2004;42:5854-6 pubmed publisher
  38. Parsot C, Mekalanos J. Structural analysis of the acfA and acfD genes of Vibrio cholerae: effects of DNA topology and transcriptional activators on expression. J Bacteriol. 1992;174:5211-8 pubmed
    ..In contrast, the expression of the acfA promoter was activated 10-fold and that of the acfD promoter was activated 3-fold by ToxT, supporting the previously proposed cascade model for organization of the ToxR regulon. ..
  39. Kovach M, Shaffer M, Peterson K. A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae. Microbiology. 1996;142 ( Pt 8):2165-74 pubmed publisher
    ..The fact that the integrase gene is absent in avirulent strains suggests that epidemic strains of V. cholerae obtained the TCP-ACF colonization gene cluster via horizontal transfer...
  40. Hu S, Peek J, Rattigan E, Taylor R, Martin J. Structure of TcpG, the DsbA protein folding catalyst from Vibrio cholerae. J Mol Biol. 1997;268:137-46 pubmed publisher
  41. Klose K, Mekalanos J. Distinct roles of an alternative sigma factor during both free-swimming and colonizing phases of the Vibrio cholerae pathogenic cycle. Mol Microbiol. 1998;28:501-20 pubmed
    ..cholerae life cycle, and some as yet unidentified gene(s) that aid colonization within the host...
  42. Mandal M, Lee M, Barrick J, Weinberg Z, Emilsson G, Ruzzo W, et al. A glycine-dependent riboswitch that uses cooperative binding to control gene expression. Science. 2004;306:275-9 pubmed publisher
    ..Thus, riboswitches perform key regulatory roles and exhibit complex performance characteristics that previously had been observed only with protein factors...
  43. De Silva R, Kovacikova G, Lin W, Taylor R, Skorupski K, Kull F. Crystal structure of the virulence gene activator AphA from Vibrio cholerae reveals it is a novel member of the winged helix transcription factor superfamily. J Biol Chem. 2005;280:13779-83 pubmed publisher
    ..The structure of AphA also provides insights into how it cooperates with AphB to activate transcription, most likely by forming a heterotetrameric complex at the tcpPH promoter...
  44. Kudryashov D, Durer Z, Ytterberg A, Sawaya M, Pashkov I, Prochazkova K, et al. Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin. Proc Natl Acad Sci U S A. 2008;105:18537-42 pubmed publisher
  45. Martinez R, Dharmasena M, Kirn T, Taylor R. Characterization of two outer membrane proteins, FlgO and FlgP, that influence vibrio cholerae motility. J Bacteriol. 2009;191:5669-79 pubmed publisher
    ..cholerae O1 of the classical biotype. This study reveals a novel mechanism for which the outer membrane proteins FlgO and FlgP function in motility to mediate flagellar stability and influence attachment and colonization...
  46. Kim Y, Zhou M, Moy S, Morales J, Cunningham M, Joachimiak A. High-resolution structure of the nitrile reductase QueF combined with molecular simulations provide insight into enzyme mechanism. J Mol Biol. 2010;404:127-37 pubmed publisher
    ..The crystal structure also suggests that two conserved residues (His233 and Asp102) play an important role in the delivery of a fourth proton to the substrate...
  47. Wang H, Wu J, Ayala J, Benitez J, Silva A. Interplay among cyclic diguanylate, HapR, and the general stress response regulator (RpoS) in the regulation of Vibrio cholerae hemagglutinin/protease. J Bacteriol. 2011;193:6529-38 pubmed publisher
    ..Based on the above findings, we propose a model for the interplay between HapR, RpoS, and c-di-GMP in the regulation of HA/protease expression...
  48. Dolores J, Satchell K. Analysis of Vibrio cholerae genome sequences reveals unique rtxA variants in environmental strains and an rtxA-null mutation in recent altered El Tor isolates. MBio. 2013;4:e00624 pubmed publisher
    ..A similar inactivation occurred during classical cholera pandemics, highlighting that evolution of El Tor cholera is following a similar path of increased dependence on cholera toxin, while eliminating other secreted factors...
  49. Ferraroni M, Del Prete S, Vullo D, Capasso C, Supuran C. Crystal structure and kinetic studies of a tetrameric type II β-carbonic anhydrase from the pathogenic bacterium Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 2015;71:2449-56 pubmed publisher
  50. Litwin C, Boyko S, Calderwood S. Cloning, sequencing, and transcriptional regulation of the Vibrio cholerae fur gene. J Bacteriol. 1992;174:1897-903 pubmed
    ..These studies identify a gene in V. cholerae homologous in both function and sequence to the fur gene of E. coli, and we have designated this gene the fur gene of V. cholerae...
  51. Faast R, Ogierman M, Stroeher U, Manning P. Nucleotide sequence of the structural gene, tcpA, for a major pilin subunit of Vibrio cholerae. Gene. 1989;85:227-31 pubmed
    ..The TcpA protein shows some similarities to the major pilins of the methylated phenylalanine type or type-4 pili from other bacteria; however, it is sufficiently different that it may represent a new class...
  52. Morona R, Stroeher U, Karageorgos L, Brown M, Manning P. A putative pathway for biosynthesis of the O-antigen component, 3-deoxy-L-glycero-tetronic acid, based on the sequence of the Vibrio cholerae O1 rfb region. Gene. 1995;166:19-31 pubmed
    ..A biosynthetic pathway for the Vc O-antigen component 3-deoxy-L-glycero-tetronic acid, based on the enzymatic functions predicted for the RfbK, RfbL, RfbM, RfbN and RfbO proteins, is presented...
  53. Vimont S, Dumontier S, Escuyer V, Berche P. The rfaD locus: a region of rearrangement in Vibrio cholerae O139. Gene. 1997;185:43-7 pubmed
    ..cholerae O1, resulting in the emergence of V. cholerae O139. The putative source of exogenous DNA might have been V. cholerae O22, O141 and O155. ..
  54. Karaolis D, Johnson J, Bailey C, Boedeker E, Kaper J, Reeves P. A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Proc Natl Acad Sci U S A. 1998;95:3134-9 pubmed
    ..As the PAI contains the CTXPhi receptor, it may represent the initial genetic factor required for the emergence of epidemic and pandemic cholera. We propose to call this island VPI (V. cholerae pathogenicity island)...
  55. H se C, Mekalanos J. Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 1999;96:3183-7 pubmed
    ..Thus, the regulation of the main virulence factors of V. cholerae appears to be modulated by endogenous and exogenous sodium levels in a complex way...
  56. Banerjee R, Das S, Mukhopadhyay K, Nag S, Chakrabortty A, Chaudhuri K. Involvement of in vivo induced cheY-4 gene of Vibrio cholerae in motility, early adherence to intestinal epithelial cells and regulation of virulence factors. FEBS Lett. 2002;532:221-6 pubmed
    ..In contrast to the cheY-4 null mutant, duplication of cheY-4 gene resulted in increased expression of ctxAB and tcpA, the two major virulence genes of V. cholerae...
  57. Lupardus P, Shen A, Bogyo M, Garcia K. Small molecule-induced allosteric activation of the Vibrio cholerae RTX cysteine protease domain. Science. 2008;322:265-8 pubmed publisher
    ..Biochemical and kinetic analyses of CPD mutants indicate that InsP6 binding induces an allosteric switch that leads to the autoprocessing and intracellular release of toxin-effector domains...
  58. Dasgupta S, Basu P, Pal R, Bag S, Bhadra R. Genetic and mutational characterization of the small alarmone synthetase gene relV of Vibrio cholerae. Microbiology. 2014;160:1855-66 pubmed publisher
    ..Since the minimal functional length of RelV was found to be 189 aa, which includes the 94 aa long RSD region, it seems that the flanking residues of the RSD are also important for maintaining the (p)ppGpp synthetase activity. ..
  59. Sakharwade S, Mukhopadhaya A. Vibrio cholerae porin OmpU induces LPS tolerance by attenuating TLR-mediated signaling. Mol Immunol. 2015;68:312-24 pubmed publisher
    ..7 cells, and down-regulates LPS-induced cell surface expression of co-stimulatory molecules. Altogether, OmpU causes suppression of LPS-mediated responses by attenuating the LPS-mediated TLR signaling pathway. ..
  60. Parsot C, Mekalanos J. Expression of ToxR, the transcriptional activator of the virulence factors in Vibrio cholerae, is modulated by the heat shock response. Proc Natl Acad Sci U S A. 1990;87:9898-902 pubmed
    ..coli heat shock promoters was detected upstream from the V. cholerae htpG gene. We propose a model in which the regulation of toxR expression by temperature is controlled by the level of sigma-32 (RpoH) RNA polymerase...
  61. Duffy L, Peterson J, Kurosky A. Isolation and characterization of a precursor form of the 'A' subunit of cholera toxin. FEBS Lett. 1981;126:187-90 pubmed
  62. McNally T, Huang Q, Janis R, Liu Z, Olejniczak E, Reilly R. Structural analysis of UBL5, a novel ubiquitin-like modifier. Protein Sci. 2003;12:1562-6 pubmed publisher
    ..In addition, we have confirmed an earlier report of an interaction between UBL5 and the cyclin-like kinase, CLK4, which we have determined is specific and does not extend to other cyclin-like kinase family members...
  63. Fujinaga Y, Wolf A, Rodighiero C, Wheeler H, Tsai B, Allen L, et al. Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm. Mol Biol Cell. 2003;14:4783-93 pubmed publisher
    ..Our results explain why LTIIb does not cause disease in humans and suggest that gangliosides with high affinity for lipid rafts may provide a general vehicle for the transport of toxins to the ER...
  64. Habibian R, Dzioba J, Barrett J, Galperin M, Loewen P, Dibrov P. Functional analysis of conserved polar residues in Vc-NhaD, Na+/H+ antiporter of Vibrio cholerae. J Biol Chem. 2005;280:39637-43 pubmed publisher
  65. Sheikh M, Potter J, Johnson K, Sim R, Boyd E, Taylor G. Crystal structure of VC1805, a conserved hypothetical protein from a Vibrio cholerae pathogenicity island, reveals homology to human p32. Proteins. 2008;71:1563-71 pubmed publisher
  66. Divya M, Sivakumar K, Sarada Devi K, Remadevi S, Thomas S. Novel multiple mutations in the topoisomerase gene of Haitian variant Vibrio cholerae O1. Antimicrob Agents Chemother. 2014;58:4982-3 pubmed publisher
  67. Michetti D, Brandsdal B, Bon D, Isaksen G, Tiberti M, Papaleo E. A comparative study of cold- and warm-adapted Endonucleases A using sequence analyses and molecular dynamics simulations. PLoS ONE. 2017;12:e0169586 pubmed publisher
    ..This makes T120V an amenable candidate for future experimental mutagenesis. ..
  68. Hughes K, Everiss K, Kovach M, Peterson K. Isolation and characterization of the Vibrio cholerae acfA gene, required for efficient intestinal colonization. Gene. 1995;156:59-61 pubmed
    ..The T7 expression system also showed that, in the presence of known SPI inhibitors, a 25-kDa unprocessed form of AcfA is produced. ..
  69. Merritt E, Kuhn P, Sarfaty S, Erbe J, Holmes R, Hol W. The 1.25 A resolution refinement of the cholera toxin B-pentamer: evidence of peptide backbone strain at the receptor-binding site. J Mol Biol. 1998;282:1043-59 pubmed publisher
  70. Das S, Chakrabortty A, Banerjee R, Chaudhuri K. Involvement of in vivo induced icmF gene of Vibrio cholerae in motility, adherence to epithelial cells, and conjugation frequency. Biochem Biophys Res Commun. 2002;295:922-8 pubmed
    ..We propose that the increased adherence to epithelial cell line and increased conjugation frequency of the mutant result from some sort of cell surface reorganization...
  71. Huda M, Chen J, Morita Y, Kuroda T, Mizushima T, Tsuchiya T. Gene cloning and characterization of VcrM, a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1. Microbiol Immunol. 2003;47:419-27 pubmed
    ..Efflux of acriflavine due to VcrM was dependent on Na+ or Li+. Moreover, Na+ efflux was observed with VcrM when TPPCl was added to Na+-loaded cells. Therefore, we conclude that VcrM is a Na+/drug antiporter-type multidrug efflux pump...
  72. O Neal C, Amaya E, Jobling M, Holmes R, Hol W. Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism. Biochemistry. 2004;43:3772-82 pubmed publisher
    ..On the basis of these six new views of the CT holotoxin, we propose a model for how the activational modifications experienced by wild-type CT are communicated to the active site...
  73. Stroeher U, Karageorgos L, Morona R, Manning P. Serotype conversion in Vibrio cholerae O1. Proc Natl Acad Sci U S A. 1992;89:2566-70 pubmed
    ..It exists as a separate transcript from the rest of the rfb region, and the distinctive G + C content of rfbT suggests that it has been acquired from a non-Vibrio source...
  74. Zhang Z, Merritt E, Ahn M, Roach C, Hou Z, Verlinde C, et al. Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin. J Am Chem Soc. 2002;124:12991-8 pubmed
    ..These results reiterate the power of the structure-based design of multivalent protein ligands as a general strategy for achieving high affinity and potent inhibition...
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    ..cholerae strains. The phenotypes of these mutants showed that this DNA region is involved in MSHA production, but is not required for general extracellular protein secretion...
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    ..A potential promoter with a sequence homologous to that of cAMP-CRP-activated promoters in E. coli was identified upstream of ORF3, the gene preceding mshA...
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    ..cholerae chemotaxis machinery. The data presented in this report provide preliminary evidence that acfB encodes an environmental sensor/signal-transducing protein involved in V. cholerae colonization...
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    ..These data suggest that this region of HutA is involved in heme binding...
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    ..These results indicate that ChiA and cholera toxin have functionally similar extracellular transport signals that are essential for eps-dependent secretion...
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    ..Thus, CTXphi, like the E. coli filamentous phages, uses both a pilus and TolQRA to enter its host. This suggests that the pathway for filamentous phage entry into cells is conserved between host bacterial species...
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    ..cholerae. The ppk null mutant of V. cholerae has diminished adaptation to high concentrations of calcium in the medium as well as motility and abiotic surface attachment...
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    ..We hypothesize that these VPI sequence differences provide preliminary evidence to help explain the differences in virulence factor expression between epidemic strains (i.e., the biotypes) of V. cholerae...
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    ..Moreover, the application of the Sawyer's test and split decomposition to detect intragenic recombination in the sequenced gene fragments did not indicate the existence of recombination in our O139 population...
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    ..The observed binding interactions can be exploited in the design of improved toxin binding inhibitors...
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    ..The discovery of the sialic acid binding site in the N-lectin-like domain suggests that this might help target VCNA to sialic acid-rich environments, thereby enhancing the catalytic efficiency of the enzyme...
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    ..The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs...