Shigella flexneri 2a str. 301

Summary

Alias: Shigella flexneri serotype 2a str. 301

Top Publications

  1. Tobe T, Yoshikawa M, Mizuno T, Sasakawa C. Transcriptional control of the invasion regulatory gene virB of Shigella flexneri: activation by virF and repression by H-NS. J Bacteriol. 1993;175:6142-9 pubmed
  2. Goldberg M, Barzu O, Parsot C, Sansonetti P. Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement. J Bacteriol. 1993;175:2189-96 pubmed
    ..Localization of IcsA within the tail at a distance from the bacterium would require its secretion; we demonstrate here that in vitro IcsA is secreted into the culture supernatant in a cleaved form. ..
  3. Brandon L, Goehring N, Janakiraman A, Yan A, Wu T, Beckwith J, et al. IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed. Mol Microbiol. 2003;50:45-60 pubmed
    ..Based on these data, we propose a model for coordinate polar targeting and secretion of IcsA at the bacterial pole. ..
  4. Le Gall T, Mavris M, Martino M, Bernardini M, Denamur E, Parsot C. Analysis of virulence plasmid gene expression defines three classes of effectors in the type III secretion system of Shigella flexneri. Microbiology. 2005;151:951-62 pubmed
    ..The differential regulation of expression of TTS effectors in response to the TTS apparatus activity suggests that different effectors might be required at different times following contact of bacteria with host cells. ..
  5. Arbibe L, Kim D, Batsche E, Pedron T, Mateescu B, Muchardt C, et al. An injected bacterial effector targets chromatin access for transcription factor NF-kappaB to alter transcription of host genes involved in immune responses. Nat Immunol. 2007;8:47-56 pubmed
    ..S. flexneri has thus evolved the capacity to precisely modulate host cell epigenetic 'information' as a strategy for repressing innate immunity. ..
  6. Menard R, Sansonetti P, Parsot C. The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD. EMBO J. 1994;13:5293-302 pubmed
    ..We propose that IpaB and IpaD, by interacting in the secretion apparatus, modulate secretion. ..
  7. Henderson I, Czeczulin J, Eslava C, Noriega F, Nataro J. Characterization of pic, a secreted protease of Shigella flexneri and enteroaggregative Escherichia coli. Infect Immun. 1999;67:5587-96 pubmed
    ..Functional analysis of the Pic protein implicates this factor in mucinase activity, serum resistance, and hemagglutination. Our data suggest that Pic may be a multifunctional protein involved in enteric pathogenesis. ..
  8. Al Hasani K, Henderson I, Sakellaris H, Rajakumar K, Grant T, Nataro J, et al. The sigA gene which is borne on the she pathogenicity island of Shigella flexneri 2a encodes an exported cytopathic protease involved in intestinal fluid accumulation. Infect Immun. 2000;68:2457-63 pubmed
    ..SigA was at least partly responsible for the ability of S. flexneri to stimulate fluid accumulation in ligated rabbit ileal loops. ..
  9. Al Hasani K, Rajakumar K, Bulach D, Robins Browne R, Adler B, Sakellaris H. Genetic organization of the she pathogenicity island in Shigella flexneri 2a. Microb Pathog. 2001;30:1-8 pubmed
    ..coli. The ShET1 enterotoxin genes, associated predominantly with S. flexneri 2a strains, are also located on the she PAI. ..

More Information

Publications171 found, 100 shown here

  1. Alto N, Shao F, Lazar C, Brost R, Chua G, Mattoo S, et al. Identification of a bacterial type III effector family with G protein mimicry functions. Cell. 2006;124:133-45 pubmed
    ..The activities of IpgB2, IpgB1, and Map are dependent on an invariant WxxxE motif found in numerous effectors leading to the speculation that they all function by a similar molecular mechanism. ..
  2. Prosseda G, Fradiani P, Di Lorenzo M, Falconi M, Micheli G, Casalino M, et al. A role for H-NS in the regulation of the virF gene of Shigella and enteroinvasive Escherichia coli. Res Microbiol. 1998;149:15-25 pubmed
    ..Our results strongly suggest that H-NS controls virF expression by binding to the virF promoter and by repressing its expression at low temperature and at low pH...
  3. Prosseda G, Falconi M, Giangrossi M, Gualerzi C, Micheli G, Colonna B. The virF promoter in Shigella: more than just a curved DNA stretch. Mol Microbiol. 2004;51:523-37 pubmed publisher
  4. Zurawski D, Mitsuhata C, Mumy K, McCormick B, Maurelli A. OspF and OspC1 are Shigella flexneri type III secretion system effectors that are required for postinvasion aspects of virulence. Infect Immun. 2006;74:5964-76 pubmed
    ..Ultimately, OspF and OspC1 are essential for PMN transepithelial migration, a phenotype associated with increased inflammation and bacterial access to the submucosa, which are fundamental aspects of S. flexneri pathogenesis...
  5. Yoshida S, Handa Y, Suzuki T, Ogawa M, Suzuki M, Tamai A, et al. Microtubule-severing activity of Shigella is pivotal for intercellular spreading. Science. 2006;314:985-9 pubmed publisher
    ..Degradation of microtubules by VirA was dependent on its alpha-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells...
  6. Yang J, Nie H, Chen L, Zhang X, Yang F, Xu X, et al. Revisiting the molecular evolutionary history of Shigella spp. J Mol Evol. 2007;64:71-9 pubmed publisher
    ..coli multiple times during a prolonged period of time, resulting in Shigella species with diverse genomes but similar pathogenic properties...
  7. Bernardini M, Mounier J, d Hauteville H, Coquis Rondon M, Sansonetti P. Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci U S A. 1989;86:3867-71 pubmed
  8. Lett M, Sasakawa C, Okada N, Sakai T, Makino S, Yamada M, et al. virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence. J Bacteriol. 1989;171:353-9 pubmed
    ..The nucleotide sequence of 4,472 bp, which contains the functional virG gene and its own regulatory sequence, was determined, and a large open reading frame encoding 1,102 amino acid residues was identified. ..
  9. Amemura M, Makino K, Shinagawa H, Nakata A. Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon. J Bacteriol. 1986;168:294-302 pubmed
    ..The 450-amino-acid ORF4 protein contains long hydrophobic regions and is likely to be a membrane protein...
  10. Freestone P, Grant S, Toth I, Norris V. Identification of phosphoproteins in Escherichia coli. Mol Microbiol. 1995;15:573-80 pubmed
    ..In addition, a calcium-stimulated protein of 70 kDa was identified as the heat-shock protein DnaK, and a 17 kDa lipid-stimulated phosphoprotein as nucleotide diphosphate kinase...
  11. Fahrner K, Block S, Krishnaswamy S, Parkinson J, Berg H. A mutant hook-associated protein (HAP3) facilitates torsionally induced transformations of the flagellar filament of Escherichia coli. J Mol Biol. 1994;238:173-86 pubmed
    ..The N-terminal sequence of HAP3 was found to be similar to the N-terminal sequence of flagellin, and the possibility that it provides a nucleation site for the C-terminal region of flagellin is discussed. ..
  12. Takeda K, Miyatake H, Yokota N, Matsuyama S, Tokuda H, Miki K. Crystal structures of bacterial lipoprotein localization factors, LolA and LolB. EMBO J. 2003;22:3199-209 pubmed publisher
    ..The results are discussed in the context of the current model for the lipoprotein transfer from the inner to the outer membrane through a hydrophilic environment...
  13. Masai H, Bond M, Arai K. Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication. Proc Natl Acad Sci U S A. 1986;83:1256-60 pubmed
    ..These results indicate that protein i is encoded by dnaT and that it is essential for chromosomal DNA replication and is involved in the induction of stable DNA replication during the SOS response...
  14. Baudry B, Kaczorek M, Sansonetti P. Nucleotide sequence of the invasion plasmid antigen B and C genes (ipaB and ipaC) of Shigella flexneri. Microb Pathog. 1988;4:345-57 pubmed
    ..The surprising A and T richness of the three genes as compared with the Escherichia coli and Shigella genomes, resulted in a biased codon usage, and raises the question of the origin of the sequences. ..
  15. Westh Hansen S, Jensen N, Munch Petersen A. Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur J Biochem. 1987;168:385-91 pubmed
    ..333 kDa, was deduced to be the coding region of nupG. Minicell-forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane-bound polypeptide with an apparent molecular mass of approximately 43 kDa...
  16. Komine Y, Kitabatake M, Yokogawa T, Nishikawa K, Inokuchi H. A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli. Proc Natl Acad Sci U S A. 1994;91:9223-7 pubmed
    ..A pair could be overcome by the introduction of a gene encoding alanyl-tRNA synthetase (alaS) on a multicopy plasmid. The evidence suggests that the proposed structural features of 10Sa RNA are indeed manifested in vivo...
  17. Koh Y, Roe J. Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS locus in Escherichia coli. J Bacteriol. 1995;177:2673-8 pubmed
    ..Northern (RNA) and S1 analyses indicated that there are two kinds of pqi-5 transcript; one covers ORF-A only and the other covers ORF-A and possibly also ORF-B...
  18. Wolodko W, Fraser M, James M, Bridger W. The crystal structure of succinyl-CoA synthetase from Escherichia coli at 2.5-A resolution. J Biol Chem. 1994;269:10883-90 pubmed
    ..A short segment of the beta-subunit from one alpha beta-dimer is in close proximity to the CoA-binding site of the other alpha beta-dimer, providing a possible rationale for the overall tetrameric structure. ..
  19. Macpherson D, Manning P, Morona R. Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri. Mol Microbiol. 1994;11:281-92 pubmed
    ..A reporter plasmid to detect genes encoding enzymes of the dTDP-rhamnose pathway is described. An analysis of the intergenic region between galF and rfbB has been made, and comparison with the same region from S. enterica LT2 discussed. ..
  20. Roderick S, Matthews B. Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme. Biochemistry. 1993;32:3907-12 pubmed
    ..In terms of both the novel backbone fold and the constitution of the active site, MAP appears to represent a new class of proteolytic enzyme...
  21. Volz K. A test case for structure-based functional assignment: the 1.2 A crystal structure of the yjgF gene product from Escherichia coli. Protein Sci. 1999;8:2428-37 pubmed publisher
    ..The lessons learned from this attempt to deduce function from structure may be useful to future projects in structural genomics...
  22. Sol M, L pez Hern ndez E, Cronet P, Lacroix E, Serrano L, Coll M, et al. Towards understanding a molecular switch mechanism: thermodynamic and crystallographic studies of the signal transduction protein CheY. J Mol Biol. 2000;303:213-25 pubmed publisher
  23. Yao X, Wang H, Shi Z, Yan X, Feng E, Yang B, et al. Identification of RanBMP interacting with Shigella flexneri IpaC invasin by two-hybrid system of yeast. World J Gastroenterol. 2003;9:1347-51 pubmed
    ..These results provide evidence that IpaC may be involved in the invasion process of S. flexneri by interacting with RanBPM, and RanBPM is most likely to be the downstream target of IpaC in the cascade events of S. flexneri infection. ..
  24. Li H, Xu H, Zhou Y, Zhang J, Long C, Li S, et al. The phosphothreonine lyase activity of a bacterial type III effector family. Science. 2007;315:1000-3 pubmed publisher
    ..This unexpected enzymatic activity, termed phosphothreonine lyase, appeared specific for MAPKs and was shared by other OspF family members...
  25. Klink B, Barden S, Heidler T, Borchers C, Ladwein M, Stradal T, et al. Structure of Shigella IpgB2 in complex with human RhoA: implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry. J Biol Chem. 2010;285:17197-208 pubmed publisher
    ..These data refine our understanding of the mode of action not only of WXXXE GEFs but also of mammalian GEFs of the DH/PH family...
  26. Tse Dinh Y, Wang J. Complete nucleotide sequence of the topA gene encoding Escherichia coli DNA topoisomerase I. J Mol Biol. 1986;191:321-31 pubmed
    ..Mapping of promoters by deletion of sequences upstream from the ATG initiation codon indicates the existence of at least two promoters that direct transcription into topA...
  27. Fenton W, Kashi Y, Furtak K, Horwich A. Residues in chaperonin GroEL required for polypeptide binding and release. Nature. 1994;371:614-9 pubmed publisher
    ..A highly conserved residue, Asp 87, positioned within a putative nucleotide-binding pocket in the top of the equatorial domain, is essential for ATP hydrolysis and polypeptide release...
  28. Daughdrill G, Chadsey M, Karlinsey J, Hughes K, Dahlquist F. The C-terminal half of the anti-sigma factor, FlgM, becomes structured when bound to its target, sigma 28. Nat Struct Biol. 1997;4:285-91 pubmed
    ..We postulate that the lack of structure in free and bound FlgM is important to its role as an exported protein...
  29. Xiang S, Short S, Wolfenden R, Carter C. The structure of the cytidine deaminase-product complex provides evidence for efficient proton transfer and ground-state destabilization. Biochemistry. 1997;36:4768-74 pubmed publisher
  30. Gehring A, Bradley K, Walsh C. Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate. Biochemistry. 1997;36:8495-503 pubmed publisher
  31. Moche M, Schneider G, Edwards P, Dehesh K, Lindqvist Y. Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase. J Biol Chem. 1999;274:6031-4 pubmed
    ..Cerulenin is covalently attached to the active site cysteine through its C2 carbon atom. The fit of the inhibitor to the active site is not optimal, and there is thus room for improvement through structure based design...
  32. Endrizzi J, Beernink P, Alber T, Schachman H. Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: implications for allosteric regulation. Proc Natl Acad Sci U S A. 2000;97:5077-82 pubmed
    ..Consequently, the conformational changes attributable to the allosteric transition of the holoenzyme remain to be defined...
  33. Matak Vinkovic D, Vinkovic M, Saldanha S, Ashurst J, von Delft F, Inoue T, et al. Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism. Biochemistry. 2001;40:14493-500 pubmed
    ..A model for the ternary enzyme-NADPH-ketopantoate ternary complex provides a rationale for kinetic data reported for specific site-directed mutants. ..
  34. Santapaola D, Del Chierico F, Petrucca A, Uzzau S, Casalino M, Colonna B, et al. Apyrase, the product of the virulence plasmid-encoded phoN2 (apy) gene of Shigella flexneri, is necessary for proper unipolar IcsA localization and for efficient intercellular spread. J Bacteriol. 2006;188:1620-7 pubmed publisher
    ..Apyrase may be important in a deoxynucleoside triphosphate-hydrolyzing activity-independent manner, suggesting that it may act as an interaction partner in the process of IcsA localization...
  35. May K, Morona R. Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. J Bacteriol. 2008;190:4666-76 pubmed publisher
    ..Using this approach, we have identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment...
  36. Botteaux A, Sory M, Biskri L, Parsot C, Allaoui A. MxiC is secreted by and controls the substrate specificity of the Shigella flexneri type III secretion apparatus. Mol Microbiol. 2009;71:449-60 pubmed publisher
    ..Although with a 10-fold reduced efficiency compared with the wild-type strain, the mxiC mutant was still able to enter epithelial cells...
  37. Lawlis V, Dennis M, Chen E, Smith D, Henner D. Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli. Appl Environ Microbiol. 1984;47:15-21 pubmed
    ..Open reading frames which, if translated, would encode proteins of molecular weights 54,000 and 52,000 were found. These were identified as the isomerase and kinase structural genes, respectively...
  38. Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H. The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I. J Mol Biol. 1993;233:109-22 pubmed publisher
    ..To some extent, the gene order correlates with the topological arrangement of the encoded subunits. The conception of modular evolution of NADH: ubiquinone oxidoreductase is further supported by the arrangement of the nuo-genes...
  39. Tung C, Joseph S, Sanbonmatsu K. All-atom homology model of the Escherichia coli 30S ribosomal subunit. Nat Struct Biol. 2002;9:750-5 pubmed publisher
    ..The hypervariable regions in our structure, which differ from the structure of the 30S ribosomal subunit from Thermus thermophilus, are consistent with the cryo-EM map of the E. coli ribosome...
  40. Chuang S, Burland V, Plunkett G, Daniels D, Blattner F. Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli. Gene. 1993;134:1-6 pubmed
    ..Both HslU and the P. haemolytica protein share a ATP/GTP-binding motif near their N-termini. The two operons described here are transcribed counterclockwise on the standard genetic map...
  41. Obmolova G, Teplyakov A, Khil P, Howard A, Camerini Otero R, Gilliland G. Crystal structure of the Escherichia coli Tas protein, an NADP(H)-dependent aldo-keto reductase. Proteins. 2003;53:323-5 pubmed
  42. Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H. Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence. J Biochem. 1985;97:993-9 pubmed
    ..C., et al. (1979) J. Bacteriol. 139, 339-345). From the deduced amino acid sequence, the secondary structure was predicted...
  43. Wallace B, Kushner S. Genetic and physical analysis of the thioredoxin (trxA) gene of Escherichia coli K-12. Gene. 1984;32:399-408 pubmed
    ..Three-factor P1 transductional crosses and physical mapping experiments have determined a map order of ilv-trxA-uvrD-corA-metE. ..
  44. Vyas N, Vyas M, Quiocho F. The 3 A resolution structure of a D-galactose-binding protein for transport and chemotaxis in Escherichia coli. Proc Natl Acad Sci U S A. 1983;80:1792-6 pubmed
    ..The four major hydrophobic clusters that stabilize the structure of the L-arabinose-binding protein are also present in the D-galactose-binding protein. ..
  45. Konigsberg W, Henderson L. Amino acid sequence of the catalytic subunit of aspartate transcarbamoylase from Escherichia coli. Proc Natl Acad Sci U S A. 1983;80:2467-71 pubmed
    ..The protein contains 310 amino acids and has a calculated molecular weight of 33,944. The negatively and positively charged residues are distributed uniformly, and there is no indication of charge clustering in the linear sequence. ..
  46. Schachman H, Pauza C, Navre M, Karels M, Wu L, Yang Y. Location of amino acid alterations in mutants of aspartate transcarbamoylase: Structural aspects of interallelic complementation. Proc Natl Acad Sci U S A. 1984;81:115-9 pubmed
    ..Combining these results with knowledge of the crystallographic structure of the wild-type enzyme provides a basis for tentative structural mechanisms for the observed complementation behavior of the mutant proteins. ..
  47. Schnier J, Kimura M, Foulaki K, Subramanian A, Isono K, Wittmann Liebold B. Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Proc Natl Acad Sci U S A. 1982;79:1008-11 pubmed
    ..The pattern of degenerate codon usage in the S1 gene is nonrandom and similar to that reported for other ribosomal protein genes. ..
  48. Watarai M, Tobe T, Yoshikawa M, Sasakawa C. Disulfide oxidoreductase activity of Shigella flexneri is required for release of Ipa proteins and invasion of epithelial cells. Proc Natl Acad Sci U S A. 1995;92:4927-31 pubmed
    ..These results indicated that the DsbA protein performs an essential function during the invasion of mammalian cells, by facilitating transport of the Spa32 protein across the outer membrane...
  49. Rouvi re P, de las Pe as A, Mecsas J, Lu C, Rudd K, Gross C. rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli. EMBO J. 1995;14:1032-42 pubmed
    ..Based upon homology arguments, we suggest that a gene encoding a negative regulator of sigma E activity is located immediately downstream of rpoE and may function as the target of the E sigma E inducing signal...
  50. Van Nuland N, Hangyi I, Van Schaik R, Berendsen H, van Gunsteren W, Scheek R, et al. The high-resolution structure of the histidine-containing phosphocarrier protein HPr from Escherichia coli determined by restrained molecular dynamics from nuclear magnetic resonance nuclear Overhauser effect data. J Mol Biol. 1994;237:544-59 pubmed publisher
    ..This suggests that the strain may be an artifact of crystallization conditions instead of an essential element in the phosphorylation/dephosphorylation process...
  51. Koonin E, Rudd K. A conserved domain in putative bacterial and bacteriophage transglycosylases. Trends Biochem Sci. 1994;19:106-7 pubmed
  52. Allaoui A, Sansonetti P, Parsot C. MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri lpa invasins. Mol Microbiol. 1993;7:59-68 pubmed
    ..Both YscC and PulD are involved in extracellular protein secretion. These results indicate that MxiD is an essential component of the lpa secretion apparatus...
  53. Imamura R, Yamanaka K, Ogura T, Hiraga S, Fujita N, Ishihama A, et al. Identification of the cpdA gene encoding cyclic 3',5'-adenosine monophosphate phosphodiesterase in Escherichia coli. J Biol Chem. 1996;271:25423-9 pubmed
    ..Our results suggested that regulation of intracellular concentration of cAMP is dependent not only on synthesis of cAMP but also on hydrolysis of cAMP by cAMP phosphodiesterase...
  54. Krahn J, Kim J, Burns M, Parry R, Zalkin H, Smith J. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site. Biochemistry. 1997;36:11061-8 pubmed publisher
    ..Different catalytic and inhibitory modes of divalent cation binding to the PRTase active site are revealed in the active conformer of the enzyme and in a feedback-inhibited GMP complex...
  55. Muchmore C, Krahn J, Kim J, Zalkin H, Smith J. Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Protein Sci. 1998;7:39-51 pubmed
    ..subtilis enzyme represents the metal-containing enzymes. The structure of the E. coli enzyme suggests that a common ancestor of the two enzyme subfamilies may have included an Fe-S cluster. ..
  56. Purdy G, Payne S. The SHI-3 iron transport island of Shigella boydii 0-1392 carries the genes for aerobactin synthesis and transport. J Bacteriol. 2001;183:4176-82 pubmed publisher
    ..An S. boydii aerobactin synthesis mutant, 0-1392 iucB, was constructed and was similar to the wild type in tissue culture assays of invasion and intercellular spread...
  57. Kramer R, Slagowski N, Eze N, Giddings K, Morrison M, Siggers K, et al. Yeast functional genomic screens lead to identification of a role for a bacterial effector in innate immunity regulation. PLoS Pathog. 2007;3:e21 pubmed publisher
    ..These studies demonstrate how yeast systems biology can facilitate functional characterization of pathogenic bacterial effector proteins...
  58. Weber R, Silverman P. The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component. J Mol Biol. 1988;203:467-78 pubmed
    ..Based on these structural data, we propose that CpxA functions as a trans-membrane sensory protein. The DNA sequence data also indicate that cpxA is the 3' gene of an operon...
  59. Lawther R, Wek R, Lopes J, Pereira R, Taillon B, Hatfield G. The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res. 1987;15:2137-55 pubmed
    ..This new information contributes to a more complete understanding of the overall control of the biosynthesis of isoleucine and valine...
  60. Soderberg B, Holmgren A, Branden C. Structure of oxidized thioredoxin to 4 with 5 A resolution. J Mol Biol. 1974;90:143-52 pubmed
  61. Ke H, Honzatko R, Lipscomb W. Structure of unligated aspartate carbamoyltransferase of Escherichia coli at 2.6-A resolution. Proc Natl Acad Sci U S A. 1984;81:4037-40 pubmed
  62. Plumbridge J. Co-ordinated regulation of amino sugar biosynthesis and degradation: the NagC repressor acts as both an activator and a repressor for the transcription of the glmUS operon and requires two separated NagC binding sites. EMBO J. 1995;14:3958-65 pubmed
    ..Moreover, the location of the distal NagC site suggests that this site is behaving like an upstream activating sequence (UAS)...
  63. Van Nuland N, Boelens R, Scheek R, Robillard G. High-resolution structure of the phosphorylated form of the histidine-containing phosphocarrier protein HPr from Escherichia coli determined by restrained molecular dynamics from NMR-NOE data. J Mol Biol. 1995;246:180-93 pubmed publisher
  64. Tominaga A, Mahmoud M, Mukaihara T, Enomoto M. Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella. Mol Microbiol. 1994;12:277-85 pubmed
    ..Loss of motility in Shigella seems to be evolutionarily a recent event...
  65. Zhang H, Zhao D, Revington M, Lee W, Jia X, Arrowsmith C, et al. The solution structures of the trp repressor-operator DNA complex. J Mol Biol. 1994;238:592-614 pubmed publisher
    ..Other direct H-bonds from Lys72, Ala80, Ile79, Thr83 and Arg84 to base-pair functional groups can also be formed in our solution structures...
  66. Murayama N, Shimizu H, Takiguchi S, Baba Y, Amino H, Horiuchi T, et al. Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F. J Mol Biol. 1996;256:483-502 pubmed
    ..tldD is a novel gene, but the tldE and zfiA genes proved to be the pmbA gene (production of Microcin B17) and the csrA gene (carbon storage regulator), respectively...
  67. Farewell A, Diez A, DiRusso C, Nystr m T. Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes. J Bacteriol. 1996;178:6443-50 pubmed
    ..In addition, cells carrying fadRS219N survive long-term stasis poorly, indicating that FadR-dependent alterations in fatty acid metabolism are an integral and important part of the adaptation to stationary phase...
  68. Yao X, Wei D, Soden C, Summers M, Beckett D. Structure of the carboxy-terminal fragment of the apo-biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase. Biochemistry. 1997;36:15089-100 pubmed publisher
    ..Although the overall folding of the two proteins is highly similar, small structural differences are apparent for residues of the biotin-binding loop that may be important for mediating specific protein-protein interactions...
  69. Allison T, Wood T, Briercheck D, Rastinejad F, Richardson J, Rule G. Crystal structure of the RNA-binding domain from transcription termination factor rho. Nat Struct Biol. 1998;5:352-6 pubmed
    ..The beta-barrel of rho130 is also surprisingly similar to the N-terminal beta-barrel of F1 ATPase, extending the applicability of F1 ATPase as a structural model for hexameric rho...
  70. Podobnik M, McInerney P, O Donnell M, Kuriyan J. A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases. J Mol Biol. 2000;300:353-62 pubmed publisher
    ..The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity...
  71. Phadtare S, Inouye M. Role of CspC and CspE in regulation of expression of RpoS and UspA, the stress response proteins in Escherichia coli. J Bacteriol. 2001;183:1205-14 pubmed publisher
    ..In the light of these observations, it seems plausible that CspC and CspE function as regulatory elements for the expression of stress proteins in the complex stress response network of E. coli...
  72. Wang J. A corrected quaternary arrangement of the peptidase HslV and atpase HslU in a cocrystal structure. J Struct Biol. 2001;134:15-24 pubmed
    ..Once these errors are corrected, a quaternary arrangement that is similar to those observed in the other structures emerges. ..
  73. Senchenkova S, Feng L, Yang J, Shashkov A, Cheng J, Liu D, et al. Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens. J Bacteriol. 2005;187:2551-4 pubmed
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