Pseudomonas syringae pv. tomato str. DC3000

Summary

Alias: Pseudomonas syringae DC3000, Pseudomonas syringae pv. tomato DC3000

Top Publications

  1. Brooks D, Hernández Guzmán G, Kloek A, Alarcon Chaidez F, Sreedharan A, Rangaswamy V, et al. Identification and characterization of a well-defined series of coronatine biosynthetic mutants of Pseudomonas syringae pv. tomato DC3000. Mol Plant Microbe Interact. 2004;17:162-74 pubmed
    ..thaliana is caused by the absence of the intact COR toxin. This is the first study to use biochemically and genetically defined COR mutants to address the role of COR in pathogenesis. ..
  2. Nomura K, DebRoy S, Lee Y, Pumplin N, Jones J, He S. A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science. 2006;313:220-3 pubmed
    ..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants. ..
  3. Hutcheson S, Bretz J, Sussan T, Jin S, Pak K. Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type III protein secretion in Pseudomonas syringae strains. J Bacteriol. 2001;183:5589-98 pubmed
    ..Physical interaction of HrpR and HrpS was confirmed by column-binding experiments. The results show that HrpR and HrpS physically interact to regulate the sigma(54)-dependent hrpL promoter in P. syringae strains. ..
  4. Cheng W, Munkvold K, Gao H, Mathieu J, Schwizer S, Wang S, et al. Structural analysis of Pseudomonas syringae AvrPtoB bound to host BAK1 reveals two similar kinase-interacting domains in a type III Effector. Cell Host Microbe. 2011;10:616-26 pubmed publisher
    ..BAK1 kinase activity is inhibited by AvrPtoB, and mutations at the interaction interface disrupt AvrPtoB virulence activity. These results shed light on a structural mechanism underlying host-pathogen coevolution. ..
  5. Shan L, Thara V, Martin G, Zhou J, Tang X. The pseudomonas AvrPto protein is differentially recognized by tomato and tobacco and is localized to the plant plasma membrane. Plant Cell. 2000;12:2323-2338 pubmed
    ..These findings demonstrate that AvrPto is recognized differently by the R genes in tomato and tobacco and that the recognition of AvrPto probably is associated with the plasma membrane. ..
  6. Bretz J, Losada L, Lisboa K, Hutcheson S. Lon protease functions as a negative regulator of type III protein secretion in Pseudomonas syringae. Mol Microbiol. 2002;45:397-409 pubmed
    ..These results indicate that expression of the hrp regulon and type III secretion are negatively regulated by Lon-mediated degradation of HrpR...
  7. Jamir Y, Guo M, Oh H, Petnicki Ocwieja T, Chen S, Tang X, et al. Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast. Plant J. 2004;37:554-65 pubmed
    ..The high proportion of effectors that suppress PCD suggests that suppressing plant immunity is one of the primary roles for DC3000 effectors and a central requirement for P. syringae pathogenesis. ..
  8. Lin N, Martin G. An avrPto/avrPtoB mutant of Pseudomonas syringae pv. tomato DC3000 does not elicit Pto-mediated resistance and is less virulent on tomato. Mol Plant Microbe Interact. 2005;18:43-51 pubmed
    ..Our results indicate that AvrPto and AvrPtoB have phenotypically redundant avirulence activity on Pto-expressing tomato and additive virulence activities on susceptible tomato plants. ..
  9. Schechter L, Vencato M, Jordan K, Schneider S, Schneider D, Collmer A. Multiple approaches to a complete inventory of Pseudomonas syringae pv. tomato DC3000 type III secretion system effector proteins. Mol Plant Microbe Interact. 2006;19:1180-92 pubmed
    ..syringae can be identified efficiently by bioinformatic methods; however, a precise inventory of the subset of Hops that are important in pathogenesis awaits more knowledge based on mutant phenotypes and functions within plants. ..

More Information

Publications109 found, 100 shown here

  1. Zhang J, Shao F, Li Y, Cui H, Chen L, Li H, et al. A Pseudomonas syringae effector inactivates MAPKs to suppress PAMP-induced immunity in plants. Cell Host Microbe. 2007;1:175-85 pubmed
    ..The MAPKs MPK3 and MPK6 physically interact with HopAI1 indicating that they are direct targets of HopAI1. These findings uncover a mechanism by which Pseudomonas syringae overcomes host innate immunity to promote pathogenesis. ..
  2. Shan L, He P, Li J, Heese A, Peck S, Nürnberger T, et al. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Microbe. 2008;4:17-27 pubmed publisher
    ..These findings uncover a unique strategy of bacterial pathogenesis where virulence effectors block signal transmission through a key common component of multiple MAMP-receptor complexes. ..
  3. Hann D, Rathjen J. Early events in the pathogenicity of Pseudomonas syringae on Nicotiana benthamiana. Plant J. 2007;49:607-18 pubmed
    ..This observation separates the respective mechanisms of the two effectors, and suggests that AvrPtoB may target the defence machinery directly for its suppressive effect. ..
  4. Wei C, Kvitko B, Shimizu R, Crabill E, Alfano J, Lin N, et al. A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III effector HopQ1-1 is able to cause disease in the model plant Nicotiana benthamiana. Plant J. 2007;51:32-46 pubmed
    ..syringae pathovars are limited by the complex interactions of effector repertoires with plant anti-effector surveillance systems, and they demonstrate that N. benthamiana can be a useful model host for DC3000. ..
  5. Abramovitch R, Kim Y, Chen S, Dickman M, Martin G. Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death. EMBO J. 2003;22:60-9 pubmed
    ..Thus, our results indicate that a type III effector can induce plant susceptibility to bacterial infection by inhibiting host PCD. ..
  6. Hauck P, Thilmony R, He S. A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Proc Natl Acad Sci U S A. 2003;100:8577-82 pubmed
    ..AvrPto is therefore one of the long postulated suppressors of an salicylic acid-independent, cell wall-based defense that is aimed at hrp mutant bacteria. ..
  7. Espinosa A, Guo M, Tam V, Fu Z, Alfano J. The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants. Mol Microbiol. 2003;49:377-87 pubmed
    ..The identification of HopPtoD2 as a PTP and a PCD suppressor suggests that the inactivation of MAPK pathways is a virulence strategy utilized by bacterial plant pathogens. ..
  8. Bretz J, Mock N, Charity J, Zeyad S, Baker C, Hutcheson S. A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection. Mol Microbiol. 2003;49:389-400 pubmed
    ..The results indicate that HopPtoD2 is a translocated effector with protein tyrosine phosphatase activity that modulates plant defence responses. ..
  9. Petnicki Ocwieja T, van Dijk K, Alfano J. The hrpK operon of Pseudomonas syringae pv. tomato DC3000 encodes two proteins secreted by the type III (Hrp) protein secretion system: HopB1 and HrpK, a putative type III translocator. J Bacteriol. 2005;187:649-63 pubmed
    ..Taken together, HopB1 is a type III effector and HrpK plays an important role in the TTSS and is a putative type III translocator. ..
  10. Chang J, Urbach J, Law T, Arnold L, Hu A, Gombar S, et al. A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae. Proc Natl Acad Sci U S A. 2005;102:2549-54 pubmed
    ..syringae pathovars and show that type III effector protein suites are highly variable in this pathogen, presumably reflecting the evolutionary selection imposed by the various host plants. ..
  11. Janjusevic R, Abramovitch R, Martin G, Stebbins C. A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase. Science. 2006;311:222-6 pubmed
    ..These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses. ..
  12. Badel J, Shimizu R, Oh H, Collmer A. A Pseudomonas syringae pv. tomato avrE1/hopM1 mutant is severely reduced in growth and lesion formation in tomato. Mol Plant Microbe Interact. 2006;19:99-111 pubmed
    ..These data suggest that AvrE1 acts within plant cells and promotes lesion formation and that the combined action of AvrE1 and HopM1 is particularly important in promoting bacterial growth in planta. ..
  13. Robert Seilaniantz A, Shan L, Zhou J, Tang X. The Pseudomonas syringae pv. tomato DC3000 type III effector HopF2 has a putative myristoylation site required for its avirulence and virulence functions. Mol Plant Microbe Interact. 2006;19:130-8 pubmed
    ..HopF2 contains a putative myristoylation site. Mutational analysis indicated that this site is required for plasma membrane localization and virulence and avirulence activities of HopF2. ..
  14. Fu Z, Guo M, Jeong B, Tian F, Elthon T, Cerny R, et al. A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity. Nature. 2007;447:284-8 pubmed
    ..Our results suggest a pathogenic strategy where the ADP-ribosylation of RNA-binding proteins quells host immunity by affecting RNA metabolism and the plant defence transcriptome. ..
  15. Rosebrock T, Zeng L, Brady J, Abramovitch R, Xiao F, Martin G. A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Nature. 2007;448:370-4 pubmed
    ..Various wild species of tomato were found to exhibit immunity in response to AvrPtoB(1-387 )and not to full-length AvrPtoB. Thus, by acquiring an E3 ligase domain, AvrPtoB has thwarted a highly conserved host resistance mechanism. ..
  16. Xing W, Zou Y, Liu Q, Liu J, Luo X, Huang Q, et al. The structural basis for activation of plant immunity by bacterial effector protein AvrPto. Nature. 2007;449:243-7 pubmed
    ..Together, our results show that AvrPto derepresses host defences by interacting with the two defence-inhibition loops of Pto. ..
  17. Underwood W, Zhang S, HE S. The Pseudomonas syringae type III effector tyrosine phosphatase HopAO1 suppresses innate immunity in Arabidopsis thaliana. Plant J. 2007;52:658-72 pubmed
    ..Genome-wide expression analysis revealed that expression of several well-known defense genes was suppressed in hrpA mutant-infected HopAO1 transgenic plants. ..
  18. Xiang T, Zong N, Zou Y, Wu Y, Zhang J, Xing W, et al. Pseudomonas syringae effector AvrPto blocks innate immunity by targeting receptor kinases. Curr Biol. 2008;18:74-80 pubmed
    ..The results suggest that the mechanism by which AvrPto recognizes virulence targets is linked to the evolution of Pto, which, in association with Prf, recognizes the bacterium and triggers strong resistance. ..
  19. Navarro L, Jay F, Nomura K, He S, Voinnet O. Suppression of the microRNA pathway by bacterial effector proteins. Science. 2008;321:964-7 pubmed publisher
    ..These results provide evidence that, like viruses, bacteria have evolved to suppress RNA silencing to cause disease. ..
  20. Ntoukakis V, Mucyn T, Gimenez Ibanez S, Chapman H, Gutierrez J, Balmuth A, et al. Host inhibition of a bacterial virulence effector triggers immunity to infection. Science. 2009;324:784-7 pubmed publisher
    ..Pto avoids this by phosphorylating and inactivating the AvrPtoB E3 domain. Thus, inactivation of a pathogen virulence molecule is one mechanism by which plants resist disease. ..
  21. Bogdanove A, Kim J, Wei Z, Kolchinsky P, Charkowski A, Conlin A, et al. Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. Proc Natl Acad Sci U S A. 1998;95:1325-30 pubmed
    ..DspF and AvrF are small (16 kDa and 14 kDa) and acidic with predicted amphipathic alpha helices in their C termini; they resemble chaperones for virulence factors secreted by type III secretion systems of animal pathogens...
  22. Alfano J, Charkowski A, Deng W, Badel J, Petnicki Ocwieja T, van Dijk K, et al. The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity . Proc Natl Acad Sci U S A. 2000;97:4856-61 pubmed
    ..Deletion of the Pto DC3000 EEL slightly reduces bacterial growth in tomato, whereas deletion of a large portion of the CEL strongly reduces growth and abolishes pathogenicity in tomato. ..
  23. Badel J, Nomura K, Bandyopadhyay S, Shimizu R, Collmer A, He S. Pseudomonas syringae pv. tomato DC3000 HopPtoM (CEL ORF3) is important for lesion formation but not growth in tomato and is secreted and translocated by the Hrp type III secretion system in a chaperone-dependent manner. Mol Microbiol. 2003;49:1239-51 pubmed
    ..Thus, HopPtoM is a type III effector that, among known plant pathogen effectors, is unusual in making a major contribution to the elicitation of lesion symptoms but not growth in host tomato leaves. ..
  24. DebRoy S, Thilmony R, Kwack Y, Nomura K, He S. A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. Proc Natl Acad Sci U S A. 2004;101:9927-32 pubmed
  25. Vinatzer B, Jelenska J, Greenberg J. Bioinformatics correctly identifies many type III secretion substrates in the plant pathogen Pseudomonas syringae and the biocontrol isolate P. fluorescens SBW25. Mol Plant Microbe Interact. 2005;18:877-88 pubmed
    ..Finally, using the reduced AvrRpt2(101-255) reporter, we confirmed seven predicted effectors from P. syringae pv. tomato DC3000, four from P. syringae pv. syringae B728a, and two from P. fluorescens SBW25. ..
  26. Abramovitch R, Janjusevic R, Stebbins C, Martin G. Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Proc Natl Acad Sci U S A. 2006;103:2851-6 pubmed
    ..Overall, these data reveal a unique bacterial pathogenesis strategy, where AvrPtoB manipulates the host Ub system and requires intrinsic E3 Ub ligase activity to suppress plant immunity. ..
  27. Fu Z, Guo M, Alfano J. Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin. J Bacteriol. 2006;188:6060-9 pubmed
  28. Ferreira A, Myers C, Gordon J, Martin G, Vencato M, Collmer A, et al. Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv. tomato DC3000, allows de novo reconstruction of the Hrp cis clement, and identifies novel coregulated genes. Mol Plant Microbe Interact. 2006;19:1167-79 pubmed
    ..syringae genomes, and it supports subsequent identification of effectors and other factors that likely are important to the host-specific virulence of P. syringae. ..
  29. Lin N, Martin G. Pto- and Prf-mediated recognition of AvrPto and AvrPtoB restricts the ability of diverse pseudomonas syringae pathovars to infect tomato. Mol Plant Microbe Interact. 2007;20:806-15 pubmed
  30. Oh H, Kvitko B, Morello J, Collmer A. Pseudomonas syringae lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J Bacteriol. 2007;189:8277-89 pubmed
    ..The three Hrp-associated lytic transglycosylases in DC3000 appear to have overlapping functions in contributing to T3SS functions during infection. ..
  31. Kvitko B, Ramos A, Morello J, Oh H, Collmer A. Identification of harpins in Pseudomonas syringae pv. tomato DC3000, which are functionally similar to HrpK1 in promoting translocation of type III secretion system effectors. J Bacteriol. 2007;189:8059-72 pubmed
    ..syringae T3SS translocon. ..
  32. Munkvold K, Martin M, Bronstein P, Collmer A. A survey of the Pseudomonas syringae pv. tomato DC3000 type III secretion system effector repertoire reveals several effectors that are deleterious when expressed in Saccharomyces cerevisiae. Mol Plant Microbe Interact. 2008;21:490-502 pubmed publisher
    ..HopAA1-1 colocalized with porin to yeast mitochondria and was shown to cause cell death in yeast and plants in a domain-dependent manner. These results support the use of yeast for the study of plant-pathogen effector repertoires. ..
  33. Wilton M, Subramaniam R, Elmore J, Felsensteiner C, Coaker G, Desveaux D. The type III effector HopF2Pto targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence. Proc Natl Acad Sci U S A. 2010;107:2349-54 pubmed publisher
    ..This virulence activity was not observed in plants genetically lacking RIN4. These data provide evidence that RIN4 is a major virulence target of HopF2(Pto) and that a pathogenic advantage can be conveyed by TTSEs that target RIN4. ..
  34. Jovanovic M, James E, Burrows P, Rego F, Buck M, Schumacher J. Regulation of the co-evolved HrpR and HrpS AAA+ proteins required for Pseudomonas syringae pathogenicity. Nat Commun. 2011;2:177 pubmed publisher
    ..The distinct HrpR and HrpS functionalities suggest how partial paralogue degeneration has potentially led to a novel control mechanism for EBPs and indicate subunit-specific roles for EBPs in ?(54)-RNA polymerase activation...
  35. Lidell M, Hutcheson S. Characterization of the hrpJ and hrpU operons of Pseudomonas syringae pv. syringae Pss61: similarity with components of enteric bacteria involved in flagellar biogenesis and demonstration of their role in HarpinPss secretion. Mol Plant Microbe Interact. 1994;7:488-97 pubmed
    ..typhimurium. ..
  36. Hong C, Jo S, Jeong H, Park J. Draft Genome Sequence of the Endophytic Strain Rhodococcus kyotonensis KB10, a Potential Biodegrading and Antibacterial Bacterium Isolated from Arabidopsis thaliana. Genome Announc. 2016;4: pubmed publisher
    ..This bacterium contains an ectoine biosynthesis gene cluster and has the potential to degrade nitroaromatic compounds. The identified bacterium may be a suitable biocontrol agent and degrader of environmental pollutants. ..
  37. Wen Z, Yao L, Singer S, Muhammad H, Li Z, Wang X. Constitutive heterologous overexpression of a TIR-NB-ARC-LRR gene encoding a putative disease resistance protein from wild Chinese Vitis pseudoreticulata in Arabidopsis and tobacco enhances resistance to phytopathogenic fungi and bacteria. Plant Physiol Biochem. 2017;112:346-361 pubmed publisher
    ..Taken together, these results indicate that VpTNL1 contributes to PM resistance in grapevine and provide an interesting gene target for the future amelioration of grape via breeding and/or biotechnology. ..
  38. Borodin A, Danilkovich A, Allikmets R, Rostapshov V, Chernov I. [Nucleotide sequence of of rpoB gene encoding the RNA-polymerase beta-subunit in Pseudomonas putida]. Dokl Akad Nauk SSSR. 1988;302:1261-5 pubmed
  39. Borodin A, Danilkovich A, Chernov I, Azhikina T, Rostapshov V. [Genes coding for RNA polymerase in bacteria. III. The use of modified Sanger's method for sequencing the C-terminal region of rpoB gene, N-terminal region of rpoC gene and intercistron region of RNA polymerase in Pseudomonas putida]. Bioorg Khim. 1988;14:1179-82 pubmed
    ..The Sanger method was modified and the primary structure of the SalI-C fragment of the Pseudomonas putida rpoBC operon was elucidated. ..
  40. Nikaidou N, Kamio Y, Izaki K. Expression of a pectin lyase gene in Escherichia coli from Pseudomonas marginalis N6301. Biosci Biotechnol Biochem. 1994;58:2297-8 pubmed
    ..coli. Highly conserved sequences of recA are observed among E. coli, P. fluorescens, and P. marginalis. From these results, we presume that recA is required for the expression of the pectin lyase gene in P. marginalis N6301. ..
  41. Rangaswamy V, Jiralerspong S, Parry R, Bender C. Biosynthesis of the Pseudomonas polyketide coronafacic acid requires monofunctional and multifunctional polyketide synthase proteins. Proc Natl Acad Sci U S A. 1998;95:15469-74 pubmed
    ..In this report a gene cluster encoding a pseudomonad polyketide has been completely sequenced and the deduced gene functions have been used to develop a biosynthetic scheme...
  42. Taira S, Tuimala J, Roine E, Nurmiaho Lassila E, Savilahti H, Romantschuk M. Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit. Mol Microbiol. 1999;34:737-44 pubmed
    ..Insertions in the 5' non-translated region and the first codons within the open reading frame affected mRNA production or stability and abolished protein production...
  43. Peng S, Zhang H, Gao Y, Pan X, Cao P, Li M, et al. Crystal structure of uroporphyrinogen III synthase from Pseudomonas syringae pv. tomato DC3000. Biochem Biophys Res Commun. 2011;408:576-81 pubmed publisher
    ..Mutation of Arg219 to Ala caused a decrease in enzymatic activity to about 25% that of the wild type enzyme. Our results provide the structural basis and biochemical evidence to further elucidate the catalytic mechanism of U3S. ..
  44. Misas Villamil J, Kolodziejek I, van der Hoorn R. Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels. Plant J. 2011;67:774-82 pubmed publisher
    ..Distant colonization does not require flagellin-mediated motility, and is common for P. syringae pathovars that represent different phylogroups...
  45. Ardanov P, Sessitsch A, Häggman H, Kozyrovska N, Pirttilä A. Methylobacterium-induced endophyte community changes correspond with protection of plants against pathogen attack. PLoS ONE. 2012;7:e46802 pubmed publisher
    ..Our results demonstrate that endophytic Methylobacterium spp. strains have varying effects on plant disease resistance, which can be modulated through the endophyte community of the host. ..
  46. Philmus B, Shaffer B, Kidarsa T, Yan Q, Raaijmakers J, Begley T, et al. Investigations into the Biosynthesis, Regulation, and Self-Resistance of Toxoflavin in Pseudomonas protegens Pf-5. Chembiochem. 2015;16:1782-90 pubmed publisher
    ..Toxoflavin production by P. protegens causes inhibition of several plant-pathogenic bacteria, and introduction of toxM into the toxoflavin-sensitive strain Pseudomonas syringae DC3000 results in resistance to toxoflavin.
  47. Jayaraman J, Choi S, Prokchorchik M, Choi D, Spiandore A, Rikkerink E, et al. A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response. Sci Rep. 2017;7:3557 pubmed publisher
    ..Collectively, data herein indicate that HopZ5 is a plasma membrane-localized acetyltransferase with autoacetylation activity required for avirulence. ..
  48. French C, Boonstra B, Bufton K, Bruce N. Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens. J Bacteriol. 1997;179:2761-5 pubmed
    ..STH is related to the flavoprotein disulfide oxidoreductases but lacks one of the conserved redox-active cysteine residues. The gene is highly similar to an E. coli gene of unknown function. ..
  49. Heeb S, Blumer C, Haas D. Regulatory RNA as mediator in GacA/RsmA-dependent global control of exoproduct formation in Pseudomonas fluorescens CHA0. J Bacteriol. 2002;184:1046-56 pubmed
    ..By a titration effect, RsmZ may then alleviate the repressing activity of RsmA on the expression of target mRNAs...
  50. Li C, Brown I, Mansfield J, Stevens C, Boureau T, Romantschuk M, et al. The Hrp pilus of Pseudomonas syringae elongates from its tip and acts as a conduit for translocation of the effector protein HrpZ. EMBO J. 2002;21:1909-15 pubmed publisher
    ..Our results indicate that both HrpA and HrpZ travel through the Hrp pilus, which functions as a conduit for the long-distance translocation of effector proteins...
  51. Parales R, Harwood C. Nucleotide sequence of the gyrB gene of Pseudomonas putida. Nucleic Acids Res. 1990;18:5880 pubmed
  52. Liu L, Shaw P. Characterization of dapB, a gene required by Pseudomonas syringae pv. tabaci BR2.024 for lysine and tabtoxinine-beta-lactam biosynthesis. J Bacteriol. 1997;179:507-13 pubmed
    ..These data also suggest that L-2,3,4,5-tetrahydrodipicolinate is a common intermediate for both lysine and tabtoxin biosynthesis. ..
  53. Lan L, Deng X, Zhou J, Tang X. Genome-wide gene expression analysis of Pseudomonas syringae pv. tomato DC3000 reveals overlapping and distinct pathways regulated by hrpL and hrpRS. Mol Plant Microbe Interact. 2006;19:976-87 pubmed publisher
    ..The novel genes and pathways identified by the microarray provide new insight into the bacterial functions coordinating with the TTSS...
  54. G hre V, Spallek T, H weker H, Mersmann S, Mentzel T, Boller T, et al. Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB. Curr Biol. 2008;18:1824-32 pubmed publisher
  55. Worley J, Russell A, Wexler A, Bronstein P, Kvitko B, Krasnoff S, et al. Pseudomonas syringae pv. tomato DC3000 CmaL (PSPTO4723), a DUF1330 family member, is needed to produce L-allo-isoleucine, a precursor for the phytotoxin coronatine. J Bacteriol. 2013;195:287-96 pubmed publisher
    ..This characterization of CmaL identifies a critical missing factor in coronatine production and provides a foundation for further investigation of a member of the widespread DUF1330 protein family...
  56. Su F, Villaume S, Rabenoelina F, Crouzet J, Clement C, Vaillant Gaveau N, et al. Different Arabidopsis thaliana photosynthetic and defense responses to hemibiotrophic pathogen induced by local or distal inoculation of Burkholderia phytofirmans. Photosynth Res. 2017;134:201-214 pubmed publisher
    ..Our findings indicated that the role of Bp here is not multifaceted, and relies only on priming of defense mechanisms but not on improving photosynthetic activity. ..
  57. Hosoya H, Nakamura K, Furukawa K. Promoter screening from Pseudomonas species by a promoter probe transposon and its structure. Biosci Biotechnol Biochem. 1994;58:2096-8 pubmed
    ..Another promoter (264 bp) (GC = 49.2%) isolated from P. fluorescens could be shortened to 154 bp by exonuclease deletion without any effect on its promoter activity in several Pseudomonas species. ..
  58. Soutourina O, Semenova E, Parfenova V, Danchin A, Bertin P. Control of bacterial motility by environmental factors in polarly flagellated and peritrichous bacteria isolated from Lake Baikal. Appl Environ Microbiol. 2001;67:3852-9 pubmed
    ..Thus, striking similarities observed in the two organisms suggest that these processes have evolved toward a similar regulatory mechanism in polarly flagellated and laterally flagellated (peritrichous) bacteria...
  59. Jovanovic M, Lawton E, Schumacher J, Buck M. Interplay among Pseudomonas syringae HrpR, HrpS and HrpV proteins for regulation of the type III secretion system. FEMS Microbiol Lett. 2014;356:201-11 pubmed publisher
    ..Deletion analysis of HrpR and HrpS proteins showed that C-terminal parts of HrpR and HrpS confer determinants indispensable for their self-assembly. ..
  60. Butcher B, Chakravarthy S, D Amico K, Stoos K, Filiatrault M. Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motility. BMC Microbiol. 2016;16:194 pubmed publisher
    ..Additionally, our data suggests that pyrimidines may be limited in the apoplastic space of the plant host tomato. ..
  61. Kaltdorf M, Dandekar T, Naseem M. Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity. Methods Mol Biol. 2017;1569:83-92 pubmed publisher
    ..We describe a detailed working protocol how to use the modified SQUAD-package by exemplifying the contrasting effects of auxin and cytokinins in shaping plant-pathogen interaction. ..
  62. Kim Y, Miller C, Anderson A. Transcriptional regulation by iron of genes encoding iron- and manganese-superoxide dismutases from Pseudomonas putida. Gene. 1999;239:129-35 pubmed
    ..4 kb and 1.2 kb) from the sodA operon. Our results reveal an intricate role of iron in the transcriptional regulation of both Pp sodA and sodB genes. ..
  63. Bjerkan T, Bender C, Ertesvåg H, Drabløs F, Fakhr M, Preston L, et al. The Pseudomonas syringae genome encodes a combined mannuronan C-5-epimerase and O-acetylhydrolase, which strongly enhances the predicted gel-forming properties of alginates. J Biol Chem. 2004;279:28920-9 pubmed
    ..Such a property has to our knowledge not been previously reported for an enzyme acting on a polysaccharide. ..
  64. Song E, Park Y, Chae S, Kim J, Cho H, Lee G, et al. Construction of a bacterial artificial chromosome library and characterization of hrp/hrc gene cluster of Pseudomonas syringae pathovar tagetis LMG5090. Biotechnol Lett. 2006;28:969-77 pubmed
    ..syringae pv. tomato DC3000, P. syringae pv. syringae B728a, and P. syringae pv. phaseolicola 1448A revealed that the entire hrp/hrc gene cluster of P. syringae pv. tagetis is conserved and identically arranged in all four pathovars. ..
  65. de Torres Zabala M, Truman W, Bennett M, Lafforgue G, Mansfield J, Rodriguez Egea P, et al. Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease. EMBO J. 2007;26:1434-43 pubmed publisher
    ..Our data suggest that a major virulence strategy is effector-mediated manipulation of plant hormone homeostasis, which leads to the suppression of defence responses...
  66. Nogales J, Vargas P, Farias G, Olmedilla A, Sanjuán J, Gallegos M. FleQ coordinates flagellum-dependent and -independent motilities in Pseudomonas syringae pv. tomato DC3000. Appl Environ Microbiol. 2015;81:7533-45 pubmed publisher
    ..syringae pv. tomato DC3000 to colonize tomato host plants and cause disease. ..
  67. Roine E, Saarinen J, Kalkkinen N, Romantschuk M. Purified HrpA of Pseudomonas syringae pv. tomato DC3000 reassembles into pili. FEBS Lett. 1997;417:168-72 pubmed
    ..HrpA and its degradation products were each shown to reassemble into filament structures after denaturation and renaturation showing that HrpA alone is sufficient for formation of filament structures...
  68. Arnold D, Jackson R, Fillingham A, Goss S, Taylor J, Mansfield J, et al. Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi. Microbiology. 2001;147:1171-82 pubmed publisher
    ..This conservation of flanking sequences has implications for the horizontal transfer of avirulence and virulence genes, suggesting that specific regions of the bacterial genome act as sites for their integration/excision...
  69. Guttman D, Vinatzer B, Sarkar S, Ranall M, Kettler G, Greenberg J. A functional screen for the type III (Hrp) secretome of the plant pathogen Pseudomonas syringae. Science. 2002;295:1722-6 pubmed publisher
    ..The secretion of two of these putative effectors was shown to be type III--dependent. Effectors showed high interstrain variation, supporting a role for some effectors in adaptation to different hosts...
  70. Losada L, Hutcheson S. Type III secretion chaperones of Pseudomonas syringae protect effectors from Lon-associated degradation. Mol Microbiol. 2005;55:941-53 pubmed publisher
    ..The results imply that Lon is involved at two distinct levels in the regulation of the P. syringae TTSS: regulation of assembly of the secreton and modulation of effector secretion...
  71. Leonard P, Brzozowski A, Lebedev A, Marshall C, Smith D, Verma C, et al. The 1.8 A resolution structure of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL) from Pseudomonas fluorescens, an enzyme that catalyses the transformation of feruloyl-coenzyme A to vanillin. Acta Crystallogr D Biol Crystallogr. 2006;62:1494-501 pubmed publisher
  72. Goel A, Lundberg D, Torres M, Matthews R, Akimoto Tomiyama C, Farmer L, et al. The Pseudomonas syringae type III effector HopAM1 enhances virulence on water-stressed plants. Mol Plant Microbe Interact. 2008;21:361-70 pubmed publisher
    ..syringae virulence by manipulation of ABA responses that suppress defense responses. In addition, host ABA responses enhanced by type III delivery of HopAM1 protect developing bacterial colonies inside leaves from osmotic stress...
  73. De N, Pirruccello M, Krasteva P, Bae N, Raghavan R, Sondermann H. Phosphorylation-independent regulation of the diguanylate cyclase WspR. PLoS Biol. 2008;6:e67 pubmed publisher
    ..A structural comparison reveals resemblance of the oligomeric states to assemblies of GAF domains, widely used regulatory domains in signaling molecules conserved from archaea to mammals, suggesting a similar mechanism of regulation...
  74. Guo M, Block A, Bryan C, Becker D, Alfano J. Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis. J Bacteriol. 2012;194:5054-64 pubmed publisher
    ..Thus, our results indicate that these catalases are virulence factors for DC3000 and are collectively required for pathogenesis...
  75. Palm Forster M, Eschen Lippold L, Uhrig J, Scheel D, Lee J. A novel family of proline/serine-rich proteins, which are phospho-targets of stress-related mitogen-activated protein kinases, differentially regulates growth and pathogen defense in Arabidopsis thaliana. Plant Mol Biol. 2017;95:123-140 pubmed publisher
    ..As datamining associates PRP expression profiles with hypoxia or oxidative stress and PRP-overexpressing plants have elevated levels of reactive oxygen species, PRP may connect MAPK and oxidative stress signaling. ..
  76. Durairaj K, Velmurugan P, Park J, Chang W, Park Y, Senthilkumar P, et al. Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses. Microbiol Res. 2018;206:43-49 pubmed publisher
    ..In these potent bacterial strains, we observed improved antagonistic activity against Pseudomonas syringae DC3000. These strains produced biocatalysts for plant growth promotion, and in vivo examination of Solanum ..
  77. Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol. 1995;61:1104-9 pubmed
    ..putida or its subgroups. The direct sequencing method of gyrB developed in this study provides a rapid and convenient system for bacterial identification, taxonomic analysis, and monitoring of bacteria in the natural environment...
  78. Kannan K, Janiyani K, Shivaji S, Ray M. Histidine utilisation operon (hut) is upregulated at low temperature in the antarctic psychrotrophic bacterium Pseudomonas syringae. FEMS Microbiol Lett. 1998;161:7-14 pubmed
    ..syringae, P. fluorescens and P. putida also suggested that the hut operon is expressed at an elevated level at low temperature...
  79. Shao F, Merritt P, Bao Z, Innes R, Dixon J. A Yersinia effector and a Pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis. Cell. 2002;109:575-88 pubmed
    ..These findings provide new insights into mechanisms of animal and plant pathogenesis...
  80. Wehling M, Guo M, Fu Z, Alfano J. The Pseudomonas syringae HopPtoV protein is secreted in culture and translocated into plant cells via the type III protein secretion system in a manner dependent on the ShcV type III chaperone. J Bacteriol. 2004;186:3621-30 pubmed publisher
  81. Kvitko B, Park D, Vel squez A, Wei C, Russell A, Martin G, et al. Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors. PLoS Pathog. 2009;5:e1000388 pubmed publisher
  82. Laville J, Voisard C, Keel C, Maurhofer M, D fago G, Haas D. Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco. Proc Natl Acad Sci U S A. 1992;89:1562-6 pubmed
    ..Expression of the gacA gene itself was increased in stationary phase. We propose that GacA, perhaps activated by conditions of restricted growth, functions as a global regulator of secondary metabolism in P. fluorescens...
  83. Kim K, Lee S, Lee K, Lim D. Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73. J Bacteriol. 1998;180:3692-6 pubmed
    ..Characterization of these mutants and identification of mutant genes suggested that active efflux mechanism and efficient repair of damaged membranes were important in toluene resistance...
  84. Fakhr M, Pe aloza V zquez A, Chakrabarty A, Bender C. Regulation of alginate biosynthesis in Pseudomonas syringae pv. syringae. J Bacteriol. 1999;181:3478-85 pubmed
    ..However, both the algD and algR1 upstream regions in P. syringae contained the consensus sequence recognized by sigma22, suggesting that algT is required for transcription of both genes...
  85. Keith L, Partridge J, Bender C. dnaK and the heat stress response of Pseudomonas syringae pv. glycinea. Mol Plant Microbe Interact. 1999;12:563-74 pubmed publisher
    ..Our results indicate that P. syringae pv. glycinea responds to heat shock by producing DnaK, but DnaK does not aid in acclimation to sustained elevated temperatures...
  86. Li H, Ullrich M. Characterization and mutational analysis of three allelic lsc genes encoding levansucrase in Pseudomonas syringae. J Bacteriol. 2001;183:3282-92 pubmed publisher
    ..PCR screening in various P. syringae strains with primers derived from the three characterized lsc genes demonstrated the presence of multiple Lsc isoenzymes in other P. syringae pathovars...
  87. Preston G, Bertrand N, Rainey P. Type III secretion in plant growth-promoting Pseudomonas fluorescens SBW25. Mol Microbiol. 2001;41:999-1014 pubmed
    ..While functionality of the TTSS has been demonstrated, the ecological significance of the rhizosphere-expressed TTSS of P. fluorescens SBW25 remains unclear...
  88. Pozidis C, Chalkiadaki A, Gomez Serrano A, Stahlberg H, Brown I, Tampakaki A, et al. Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization. J Biol Chem. 2003;278:25816-24 pubmed publisher
    ..We propose that TTS ATPases catalyze protein translocation as activated homo-oligomers at the plasma membrane...
  89. McCarthy C, Woods R, Beacham I. Regulation of the aprX-lipA operon of Pseudomonas fluorescens B52: differential regulation of the proximal and distal genes, encoding protease and lipase, by ompR-envZ. FEMS Microbiol Lett. 2004;241:243-8 pubmed publisher
    ..Differences in the regulation of lipase and protease may be related to the proximal and distal locations of aprX and lipA within the operon...
  90. Rodr guez Herva J, Gonz lez Melendi P, Cuartas Lanza R, Ant nez Lamas M, R o Alvarez I, Li Z, et al. A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses. Cell Microbiol. 2012;14:669-81 pubmed publisher
    ..Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant immunity responses and a target for pathogen suppression...
  91. Wang L, Guo Z, Zhang Y, Wang Y, Yang G, Yang L, et al. Overexpression of LhSorNPR1, a NPR1-like gene from the oriental hybrid lily 'Sorbonne', conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. Physiol Mol Biol Plants. 2017;23:793-808 pubmed publisher
    ..The enhanced disease resistance of LhSorNPR1 expressing plants could correlate to elevated expression levels in pathogenesis-related genes (PR1, PR2, and PR5) in vivo...
  92. Sato S, Hayashi M, Imamura S, Ozeki Y, Kawaguchi A. Primary structures of the genes, faoA and faoB, from Pseudomonas fragi B-0771 which encode the two subunits of the HDT multienzyme complex involved in fatty acid beta-oxidation. J Biochem. 1992;111:8-15 pubmed
    ..coli cells transformed with the plasmid possessing the faoA gene, suggesting that both the hydratase and dehydrogenase activities may be exhibited by the alpha-subunit of HDT.(ABSTRACT TRUNCATED AT 250 WORDS)..