Pseudomonas syringae pv. syringae B728a


Alias: Pseudomonas syringae pv. syringae str. B728a, Pseudomonas syringae pv. syringae strain B728a

Top Publications

  1. Preston G, Deng W, Huang H, Collmer A. Negative regulation of hrp genes in Pseudomonas syringae by HrpV. J Bacteriol. 1998;180:4532-7 pubmed
    ..These results suggest that HrpF, HrpG, and HrpT are all components of the type III protein secretion system whereas HrpV is a negative regulator of transcription of the Hrp regulon. ..
  2. Bretz J, Losada L, Lisboa K, Hutcheson S. Lon protease functions as a negative regulator of type III protein secretion in Pseudomonas syringae. Mol Microbiol. 2002;45:397-409 pubmed
    ..These results indicate that expression of the hrp regulon and type III secretion are negatively regulated by Lon-mediated degradation of HrpR...
  3. Jakob K, Goss E, Araki H, Van T, Kreitman M, Bergelson J. Pseudomonas viridiflava and P. syringae--natural pathogens of Arabidopsis thaliana. Mol Plant Microbe Interact. 2002;15:1195-203 pubmed publisher
    ..viridiflava isolates. We believe that these pathogens will provide a powerful system for exploring coevolution in natural plant-pathogen interactions...
  4. Wei C, Deng W, Huang H. A chaperone-like HrpG protein acts as a suppressor of HrpV in regulation of the Pseudomonas syringae pv. syringae type III secretion system. Mol Microbiol. 2005;57:520-36 pubmed
  5. Yin J, Zheng W, Gao Y, Jiang C, Shi H, Diao X, et al. Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas. iScience. 2019;14:1-14 pubmed publisher
    ..coli. The utility of these systems was demonstrated by engineering P. aeruginosa genomes to create an attenuated rhamnolipid producer. Our work enhances the potential for functional genomics in Pseudomonas. ..
  6. Yu X, Lund S, Greenwald J, Records A, Scott R, Nettleton D, et al. Transcriptional analysis of the global regulatory networks active in Pseudomonas syringae during leaf colonization. MBio. 2014;5:e01683-14 pubmed publisher
  7. Tarighi S, Taheri P. The role of a periplasmic gluconolactonase (PpgL)-like protein in Pseudomonas syringae pv. syringae B728a. World J Microbiol Biotechnol. 2011;27:1303-11 pubmed publisher
    ..Together, this work reveals the important role of the new PpgL-like protein PspL in quorum sensing of P. syringae pv. syringae B728a. ..
  8. Huang F, Tang J, He L, Ding X, Huang S, Zhang Y, et al. Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a. Microb Cell Fact. 2018;17:31 pubmed publisher
    ..coelicolor M145 and S. lividans TK24. Syringolin derivatives demonstrated high cytotoxicity in vitro and in vivo. Hence, this paper provided an important foundation for the discovery and production of new antitumor compounds. ..
  9. Lee J, Klusener B, Tsiamis G, Stevens C, Neyt C, Tampakaki A, et al. HrpZ(Psph) from the plant pathogen Pseudomonas syringae pv. phaseolicola binds to lipid bilayers and forms an ion-conducting pore in vitro. Proc Natl Acad Sci U S A. 2001;98:289-94 pubmed publisher
    ..Such pore-forming activity may allow nutrient release and/or delivery of virulence factors during bacterial colonization of host plants...

More Information

Publications140 found, 100 shown here

  1. Mukhopadhyay P, Williams J, Mills D. Molecular analysis of a pathogenicity locus in Pseudomonas syringae pv. syringae. J Bacteriol. 1988;170:5479-88 pubmed
    ..Fusion of the lacZ gene to ORF2 led to the expression of a hybrid protein inducible in Escherichia coli. The functions of the putative proteins encoded by ORF1 and ORF2 are unknown at present. ..
  2. Lüneberg E, Muller D, Steinmetz I, Frosch M. Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species. FEMS Microbiol Lett. 1997;154:131-7 pubmed
    ..aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the gamma-subclass of the Proteobacteria. ..
  3. Kim Y, Miller C, Anderson A. Transcriptional regulation by iron of genes encoding iron- and manganese-superoxide dismutases from Pseudomonas putida. Gene. 1999;239:129-35 pubmed
    ..4 kb and 1.2 kb) from the sodA operon. Our results reveal an intricate role of iron in the transcriptional regulation of both Pp sodA and sodB genes. ..
  4. Fadouloglou V, Tampakaki A, Glykos N, Bastaki M, Hadden J, Phillips S, et al. Structure of HrcQB-C, a conserved component of the bacterial type III secretion systems. Proc Natl Acad Sci U S A. 2004;101:70-5 pubmed publisher
    ..Based on the analogies between HrcQ(B) and its flagellum homologues, we propose that HrcQ(B)-C participates in the formation of a C-ring-like assembly...
  5. da Costa e Silva O, Kosuge T. Molecular characterization and expression analysis of the anthranilate synthase gene of Pseudomonas syringae subsp. savastanoi. J Bacteriol. 1991;173:463-71 pubmed
    ..savastanoi is independent of the concentration of tryptophan in the culture medium. Implications of such an expression pattern on the virulence of this bacterium are discussed. ..
  6. Borodin A, Danilkovich A, Chernov I, Azhikina T, Rostapshov V. [Genes coding for RNA polymerase in bacteria. III. The use of modified Sanger's method for sequencing the C-terminal region of rpoB gene, N-terminal region of rpoC gene and intercistron region of RNA polymerase in Pseudomonas putida]. Bioorg Khim. 1988;14:1179-82 pubmed
    ..The Sanger method was modified and the primary structure of the SalI-C fragment of the Pseudomonas putida rpoBC operon was elucidated. ..
  7. Liao C, McCallus D, Fett W. Molecular characterization of two gene loci required for production of the key pathogenicity factor pectate lyase in Pseudomonas viridiflava. Mol Plant Microbe Interact. 1994;7:391-400 pubmed
    ..Mutants of P. viridiflava strain SF312A deficient in production of Pel, Prt, and the exopolysaccharide alginate also were identified.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  8. Singer A, Wu B, Yee A, Houliston S, Xu X, Cui H, et al. Structural analysis of HopPmaL reveals the presence of a second adaptor domain common to the HopAB family of Pseudomonas syringae type III effectors. Biochemistry. 2012;51:1-3 pubmed publisher
  9. De Mot R, Proost P, Van Damme J, Vanderleyden J. Homology of the root adhesin of Pseudomonas fluorescens OE 28.3 with porin F of P. aeruginosa and P. syringae. Mol Gen Genet. 1992;231:489-93 pubmed
    ..However, a cysteine-rich domain present in the OprFs of P. aeruginosa and P. syringae is absent from the adhesin of P. fluorescens. Instead, it contains a shorter sequence with eight alternating proline residues. ..
  10. Stoddard B, Howell P, Ringe D, Petsko G. The 2.1-A resolution structure of iron superoxide dismutase from Pseudomonas ovalis. Biochemistry. 1990;29:8885-93 pubmed
    ..This residue shows the same structural interactions in both cases, implying that iron and manganese SODs are second-site revertants of one another. ..
  11. Zhang J, Quigley N, Gross D. Analysis of the syrB and syrC genes of Pseudomonas syringae pv. syringae indicates that syringomycin is synthesized by a thiotemplate mechanism. J Bacteriol. 1995;177:4009-20 pubmed
    ..In addition, a zinc-binding motif was found near the C terminus of SyrC. The data suggest that SyrB and SyrC function as peptide synthetases in a thiotemplate mechanism of syringomycin biosynthesis. ..
  12. Grimm C, Aufsatz W, Panopoulos N. The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit. Mol Microbiol. 1995;15:155-65 pubmed
    ..The hrpS transcription start site maps 179 nucleotides upstream of the initiation codon ATG, as determined by primer extension analysis, and is preceded by a typical -12/-24 promoter motif...
  13. Michel V, Lehoux I, Depret G, Anglade P, Labadie J, Hebraud M. The cold shock response of the psychrotrophic bacterium Pseudomonas fragi involves four low-molecular-mass nucleic acid-binding proteins. J Bacteriol. 1997;179:7331-42 pubmed
    ..The four peptides belong to the family of small nucleic acid-binding proteins as CspA, the major Escherichia coli Csp. They are likely to play a major role in the adaptative response of P. fragi to environmental temperature changes. ..
  14. Guenzi E, Galli G, Grgurina I, Gross D, Grandi G. Characterization of the syringomycin synthetase gene cluster. A link between prokaryotic and eukaryotic peptide synthetases. J Biol Chem. 1998;273:32857-63 pubmed
    ..This feature, together with the absence of a single transcription unit and the absence of epimerase-like domains make syringomycin synthetase more related to the eukaryotic peptide synthetases than to the bacterial counterparts...
  15. Keith L, Bender C. AlgT (sigma22) controls alginate production and tolerance to environmental stress in Pseudomonas syringae. J Bacteriol. 1999;181:7176-84 pubmed
    ..The latter two compounds are frequently encountered during colonization of plant tissue and may be unique signals for algT activation in P. syringae...
  16. De N, Pirruccello M, Krasteva P, Bae N, Raghavan R, Sondermann H. Phosphorylation-independent regulation of the diguanylate cyclase WspR. PLoS Biol. 2008;6:e67 pubmed publisher
    ..A structural comparison reveals resemblance of the oligomeric states to assemblies of GAF domains, widely used regulatory domains in signaling molecules conserved from archaea to mammals, suggesting a similar mechanism of regulation...
  17. Parales R, Harwood C. Nucleotide sequence of the gyrB gene of Pseudomonas putida. Nucleic Acids Res. 1990;18:5880 pubmed
  18. Xu G, Gross D. Physical and functional analyses of the syrA and syrB genes involved in syringomycin production by Pseudomonas syringae pv. syringae. J Bacteriol. 1988;170:5680-8 pubmed
    ..The syrA and syrB genes were required for the formation of proteins SR4 (approximately 350,000) and SR5 (approximately 130,000), which are believed to be components of the syringomycin synthetase complex. ..
  19. Venturi V, Wolfs K, Leong J, Weisbeek P. Amplification of the groESL operon in Pseudomonas putida increases siderophore gene promoter activity. Mol Gen Genet. 1994;245:126-32 pubmed
    ..The groESL operon codes for the chaperone proteins GroES and GroEL, which are responsible for mediating the folding and assembly of many proteins. ..
  20. Sundin G. Examination of base pair variants of the strA-strB streptomycin resistance genes from bacterial pathogens of humans, animals and plants. J Antimicrob Chemother. 2000;46:848-9 pubmed
  21. Heeb S, Blumer C, Haas D. Regulatory RNA as mediator in GacA/RsmA-dependent global control of exoproduct formation in Pseudomonas fluorescens CHA0. J Bacteriol. 2002;184:1046-56 pubmed
    ..By a titration effect, RsmZ may then alleviate the repressing activity of RsmA on the expression of target mRNAs...
  22. M ller S, Pozidis C, Stone R, Meesters C, Chami M, Engel A, et al. Double hexameric ring assembly of the type III protein translocase ATPase HrcN. Mol Microbiol. 2006;61:119-25 pubmed publisher
    ..0 +/- 2.0 nm high and has a 2.0-3.8 nm wide inner channel. This structure is compared to a homology model based on the structure of the F1-beta-ATPase. A model for its incorporation within the T3S apparatus is presented...
  23. Lidell M, Hutcheson S. Characterization of the hrpJ and hrpU operons of Pseudomonas syringae pv. syringae Pss61: similarity with components of enteric bacteria involved in flagellar biogenesis and demonstration of their role in HarpinPss secretion. Mol Plant Microbe Interact. 1994;7:488-97 pubmed
    ..typhimurium. ..
  24. Huang H, Xiao Y, Lin R, Lu Y, Hutcheson S, Collmer A. Characterization of the Pseudomonas syringae pv. syringae 61 hrpJ and hrpI genes: homology of HrpI to a superfamily of proteins associated with protein translocation. Mol Plant Microbe Interact. 1993;6:515-20 pubmed
    ..The products of these genes were confirmed by T7 polymerase-dependent expression and sodium dodecyl sulfate-polyacrylamide gel analysis. HrpI belongs to a superfamily of proteins represented by Yersinia pestis LcrD. ..
  25. White A, Metcalf W. Two C-P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphite, and hypophosphite. J Bacteriol. 2004;186:4730-9 pubmed publisher
    ..The substrate ranges of both C-P lyases are limited, as growth on other phosphonate compounds, including glyphosate and phenylphosphonate, was not observed...
  26. Wu H, Kosaka H, Kato J, Kuroda A, Ikeda T, Takiguchi N, et al. Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system. J Biosci Bioeng. 1999;87:273-9 pubmed
    ..putida. Although overexpression of the pstSCAB genes in P. putida PRS2000 resulted in decreased cell growth, this recombinant strain could remove Pi at a rate similar to that seen with the control strain. ..
  27. Desveaux D, Singer A, Wu A, McNulty B, Musselwhite L, Nimchuk Z, et al. Type III effector activation via nucleotide binding, phosphorylation, and host target interaction. PLoS Pathog. 2007;3:e48 pubmed publisher
    ..Our data suggest that activated AvrB, bound to RIN4, is indirectly recognized by RPM1 to initiate plant immune system function...
  28. Patel H, Ferrante P, Covaceuszach S, Lamba D, Scortichini M, Venturi V. The kiwifruit emerging pathogen Pseudomonas syringae pv. actinidiae does not produce AHLs but possesses three luxR solos. PLoS ONE. 2014;9:e87862 pubmed publisher
    ..Gene promoter analysis revealed that the three solos are not auto-regulated and investigated their possible role in several bacterial phenotypes...
  29. Harris J, Auffret A, Northrop F, Walker J. Structural comparisons of superoxide dismutases. Eur J Biochem. 1980;106:297-303 pubmed
    ..Two histidine residues are conserved in all proteins and secondary structure predictions suggest they are in close proximity in the same alpha-helix. These residues may provide ligands for the bound metal. ..
  30. Nikaidou N, Kamio Y, Izaki K. Expression of a pectin lyase gene in Escherichia coli from Pseudomonas marginalis N6301. Biosci Biotechnol Biochem. 1994;58:2297-8 pubmed
    ..coli. Highly conserved sequences of recA are observed among E. coli, P. fluorescens, and P. marginalis. From these results, we presume that recA is required for the expression of the pectin lyase gene in P. marginalis N6301. ..
  31. Huang Y, Ito J. DNA polymerase C of the thermophilic bacterium Thermus aquaticus: classification and phylogenetic analysis of the family C DNA polymerases. J Mol Evol. 1999;48:756-69 pubmed
    ..Third, the cyanobacterial family C DNA pol, classified as class III because it is encoded by a split gene, forms a group with the high-G+C Gram-positive bacteria...
  32. Musa A, Minard P, Mazzucchi U. Identification and expression of the Pseudomonas syringae pv. aptata hrpZ(Psa) gene which encodes an harpin elicitor. Antonie Van Leeuwenhoek. 2001;79:61-71 pubmed
    ..On the basis of the amino acid sequence and its ability to induce HR in tobacco leaves, it was identified as a P. syringae pv. aptata harpin. ..
  33. Jovanovic M, James E, Burrows P, Rego F, Buck M, Schumacher J. Regulation of the co-evolved HrpR and HrpS AAA+ proteins required for Pseudomonas syringae pathogenicity. Nat Commun. 2011;2:177 pubmed publisher
    ..The distinct HrpR and HrpS functionalities suggest how partial paralogue degeneration has potentially led to a novel control mechanism for EBPs and indicate subunit-specific roles for EBPs in ?(54)-RNA polymerase activation...
  34. Danilkovich A, Borodin A, Allikmets R, Rostapshov V, Chernov I. [Nucleotide sequence of the rpoC-gene coding for RNA polymerase beta'-subunit of Pseudomonas putida]. Dokl Akad Nauk SSSR. 1988;303:241-5 pubmed
  35. Isobe T, Fang Y, Muno D, Okuyama T, Ohmori D, Yamakura F. Amino acid sequence of iron-superoxide dismutase from Pseudomonas ovalis. FEBS Lett. 1987;223:92-6 pubmed
    ..The sequence information of the P. ovalis dismutase will facilitate refinement of the X-ray crystallographic data that are now available at 2.9 A resolution. ..
  36. Mazzola M, White F. A mutation in the indole-3-acetic acid biosynthesis pathway of Pseudomonas syringae pv. syringae affects growth in Phaseolus vulgaris and syringomycin production. J Bacteriol. 1994;176:1374-82 pubmed
    ..The results indicate that bacterially derived IAA, or its biosynthesis, is involved in the regulation of in planta growth and in the expression of other factors that affect the host-pathogen interaction. ..
  37. Liu L, Shaw P. Characterization of dapB, a gene required by Pseudomonas syringae pv. tabaci BR2.024 for lysine and tabtoxinine-beta-lactam biosynthesis. J Bacteriol. 1997;179:507-13 pubmed
    ..These data also suggest that L-2,3,4,5-tetrahydrodipicolinate is a common intermediate for both lysine and tabtoxin biosynthesis. ..
  38. Dekkers L, Bloemendaal C, de Weger L, Wijffelman C, Spaink H, Lugtenberg B. A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365. Mol Plant Microbe Interact. 1998;11:45-56 pubmed publisher
    ..We conclude that colonization is an active process in which an environmental stimulus, through this two-component system, activates a so far unknown trait that is crucial for colonization...
  39. Preston L, Wong T, Bender C, Schiller N. Characterization of alginate lyase from Pseudomonas syringae pv. syringae. J Bacteriol. 2000;182:6268-71 pubmed
    ..Site-directed mutagenesis of histidine and tryptophan residues at positions 204 and 207, respectively, indicated that these amino acids are critical for lyase activity. ..
  40. Sinha H, Pain A, Johnstone K. Analysis of the role of recA in phenotypic switching of Pseudomonas tolaasii. J Bacteriol. 2000;182:6532-5 pubmed
    ..It is concluded that phenotypic switching from 1116S to 1116R is recA dependent whereas that from 1116R to 1116S is recA independent...
  41. Keith L, Bender C. Genetic divergence in the algT-muc operon controlling alginate biosynthesis and response to environmental stress in Pseudomonas syringae. DNA Seq. 2001;12:125-9 pubmed
    ..syringae revealed a unique arrangement when compared to other bacteria and lacked a mucC homologue. The relative importance of the mucC gene in the algT (rpoE) operon of various bacterial species is discussed. ..
  42. Bjerkan T, Bender C, Ertesvåg H, Drabløs F, Fakhr M, Preston L, et al. The Pseudomonas syringae genome encodes a combined mannuronan C-5-epimerase and O-acetylhydrolase, which strongly enhances the predicted gel-forming properties of alginates. J Biol Chem. 2004;279:28920-9 pubmed
    ..Such a property has to our knowledge not been previously reported for an enzyme acting on a polysaccharide. ..
  43. Blasiak L, Vaillancourt F, Walsh C, Drennan C. Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis. Nature. 2006;440:368-71 pubmed publisher
    ..6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion...
  44. Mansfield J, Jenner C, Hockenhull R, Bennett M, Stewart R. Characterization of avrPphE, a gene for cultivar-specific avirulence from Pseudomonas syringae pv. phaseolicola which is physically linked to hrpY, a new hrp gene identified in the halo-blight bacterium. Mol Plant Microbe Interact. 1994;7:726-39 pubmed
    ..Sequence analysis showed that the region linked to avrPphE was very similar to DNA containing hrp genes from P. s. pv. syringae including hrpJ, hrpL, and hrpK. ..
  45. Carpena X, Soriano M, Klotz M, Duckworth H, Donald L, Melik Adamyan W, et al. Structure of the Clade 1 catalase, CatF of Pseudomonas syringae, at 1.8 A resolution. Proteins. 2003;50:423-36 pubmed
    ..A comparison of the water occupancy in this region with the same region in other catalases reveals significant differences among the catalases. ..
  46. Kang B, Cho B, Anderson A, Kim Y. The global regulator GacS of a biocontrol bacterium Pseudomonas chlororaphis O6 regulates transcription from the rpoS gene encoding a stationary-phase sigma factor and affects survival in oxidative stress. Gene. 2004;325:137-43 pubmed
    ..This RNA is implicated in the control of genes activated by the GacS system. Thus, the mechanism by which GacS mediates the activation of genes under its control requires further investigation in Pc O6. ..
  47. Yamamoto S, Wakayama M, Tachiki T. Cloning and expression of Pseudomonas taetrolens Y-30 gene encoding glutamine synthetase: an enzyme available for theanine production by coupled fermentation with energy transfer. Biosci Biotechnol Biochem. 2006;70:500-7 pubmed
    ..taetrolens Y-30. Recombinant GS had the same properties as those of unnadenylylated intrinsic GS, and formed theanine in the mixture of coupled fermentation with energy transfer. ..
  48. Berge O, Monteil C, Bartoli C, Chandeysson C, Guilbaud C, Sands D, et al. A user's guide to a data base of the diversity of Pseudomonas syringae and its application to classifying strains in this phylogenetic complex. PLoS ONE. 2014;9:e105547 pubmed publisher
    ..Finally, our analysis leads to predictions about the diversity of P. syringae that is yet to be discovered. We present here an expandable framework mainly based on cts genetic analysis into which more diversity can be integrated. ..
  49. Anzai H, Yoneyama K, Yamaguchi I. The nucleotide sequence of tabtoxin resistance gene (ttr) of Pseudomonas syringae pv. tabaci. Nucleic Acids Res. 1990;18:1890 pubmed
  50. Takagi J, Tokushige M, Shimura Y. Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens. J Biochem. 1986;100:697-705 pubmed
    ..Cys-140 and Cys-430 of the E. coli enzyme, which had been assigned as functionally essential (Ida & Tokushige (1985) J. Biochem. 98, 793-797), were substituted by Ala-140 and Ala-431, respectively, in the P. fluorescens enzyme. ..
  51. Huang H, He S, Bauer D, Collmer A. The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants. J Bacteriol. 1992;174:6878-85 pubmed
    ..These results suggest that the P. syringae pv. syringae HrpH protein is involved in the secretion of a proteinaceous HR elicitor...
  52. Xiao Y, Lu Y, Heu S, Hutcheson S. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J Bacteriol. 1992;174:1734-41 pubmed
    ..syringae 61 hrp genes at levels exceeding 0.028%. The results indicate that enhanced expression of hrp genes occurs early in the interaction with nonhost plant species in an apparent response to altered nutritional conditions...
  53. Cha J, Cooksey D. Copper resistance in Pseudomonas syringae mediated by periplasmic and outer membrane proteins. Proc Natl Acad Sci U S A. 1991;88:8915-9 pubmed
    ..One molecule of CopA bound 10.9 +/- 1.2 atoms of copper and one molecule of CopC bound 0.6 +/- 0.1 atom of copper. The Cop proteins apparently mediate sequestration of copper outside of the cytoplasm as a copper-resistance mechanism...
  54. Sundin G, Bender C. Expression of the strA-strB streptomycin resistance genes in Pseudomonas syringae and Xanthomonas campestris and characterization of IS6100 in X. campestris. Appl Environ Microbiol. 1995;61:2891-7 pubmed
    ..Furthermore, the widespread dissemination of Tn5393 and derivatives in phytopathogenic prokaryotes confirms the importance of these bacteria as reservoirs of antibiotic resistance in the environment...
  55. Rich J, Kinscherf T, Kitten T, Willis D. Genetic evidence that the gacA gene encodes the cognate response regulator for the lemA sensor in Pseudomonas syringae. J Bacteriol. 1994;176:7468-75 pubmed
    ..Southwestern (DNA-protein) analysis revealed that the lemA and gacA genes were required for the full expression of a DNA binding activity...
  56. Mills S, Lim C, Cooksey D. Purification and characterization of CopR, a transcriptional activator protein that binds to a conserved domain (cop box) in copper-inducible promoters of Pseudomonas syringae. Mol Gen Genet. 1994;244:341-51 pubmed
    ..Pcop and PcopH do not share a sequence consensus with other characterized promoters from P. syrinagae or E. coli. The results presented delineate important regions on two copper-inducible promoters form P. syringae...
  57. Xiao Y, Heu S, Yi J, Lu Y, Hutcheson S. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J Bacteriol. 1994;176:1025-36 pubmed
    ..The results indicate that hrpRS and hrpL are part of a regulatory cascade in which HrpR and HrpS activate expression of hrpL and HrpL, a putative sigma factor, induces expression of HrpL-responsive genes...
  58. Quigley N, Mo Y, Gross D. SyrD is required for syringomycin production by Pseudomonas syringae pathovar syringae and is related to a family of ATP-binding secretion proteins. Mol Microbiol. 1993;9:787-801 pubmed
    ..It is proposed that SyrD is a cytoplasmic membrane protein that functions as an ATP-driven efflux pump for the secretion of syringomycin...
  59. Runyen Janecky L, Sample A, Maleniak T, West S. A divergently transcribed open reading frame is located upstream of the Pseudomonas aeruginosa vfr gene, a homolog of Escherichia coli crp. J Bacteriol. 1997;179:2802-9 pubmed
    ..Thus, the orfX mRNA cannot hybridize to the 5' end of the vfr mRNA to inhibit vfr transcription, a mechanism that has been postulated to control crp transcription in E. coli...
  60. Sato S, Nam J, Kasuga K, Nojiri H, Yamane H, Omori T. Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10. J Bacteriol. 1997;179:4850-8 pubmed
  61. Kitten T, Kinscherf T, McEvoy J, Willis D. A newly identified regulator is required for virulence and toxin production in Pseudomonas syringae. Mol Microbiol. 1998;28:917-29 pubmed
    ..Thus, salA appears to encode a novel regulator that activates the expression of at least two separate genetic subsets of the gacS/gacA regulon, one pathway leading to syringomycin production and the other resulting in plant disease...
  62. Fakhr M, Pe aloza V zquez A, Chakrabarty A, Bender C. Regulation of alginate biosynthesis in Pseudomonas syringae pv. syringae. J Bacteriol. 1999;181:3478-85 pubmed
    ..However, both the algD and algR1 upstream regions in P. syringae contained the consensus sequence recognized by sigma22, suggesting that algT is required for transcription of both genes...
  63. Keith L, Partridge J, Bender C. dnaK and the heat stress response of Pseudomonas syringae pv. glycinea. Mol Plant Microbe Interact. 1999;12:563-74 pubmed publisher
    ..Our results indicate that P. syringae pv. glycinea responds to heat shock by producing DnaK, but DnaK does not aid in acclimation to sustained elevated temperatures...
  64. Hendrickson E, Guevera P, Pe aloza V zquez A, Shao J, Bender C, Ausubel F. Virulence of the phytopathogen Pseudomonas syringae pv. maculicola is rpoN dependent. J Bacteriol. 2000;182:3498-507 pubmed
    ..However, constitutive expression of hrpL in ES4326 rpoN::Km(r) did not restore coronatine production, showing that coronatine biosynthesis requires factors other than hrpL...
  65. Scholz Schroeder B, Hutchison M, Grgurina I, Gross D. The contribution of syringopeptin and syringomycin to virulence of Pseudomonas syringae pv. syringae strain B301D on the basis of sypA and syrB1 biosynthesis mutant analysis. Mol Plant Microbe Interact. 2001;14:336-48 pubmed publisher
    ..These data demonstrate that syringopeptin and syringomycin are major virulence determinants of P. syringae pv. syringae...
  66. Li H, Ullrich M. Characterization and mutational analysis of three allelic lsc genes encoding levansucrase in Pseudomonas syringae. J Bacteriol. 2001;183:3282-92 pubmed publisher
    ..PCR screening in various P. syringae strains with primers derived from the three characterized lsc genes demonstrated the presence of multiple Lsc isoenzymes in other P. syringae pathovars...
  67. Nojiri H, Sekiguchi H, Maeda K, Urata M, Nakai S, Yoshida T, et al. Genetic characterization and evolutionary implications of a car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10. J Bacteriol. 2001;183:3663-79 pubmed publisher
    ..In conclusion, through the above gene rearrangement, the novel genetic structure of the car gene cluster has been constructed. In addition, it was also revealed that the car and ant gene clusters are located on the megaplasmid pCAR1...
  68. Lu S, Scholz Schroeder B, Gross D. Characterization of the salA, syrF, and syrG regulatory genes located at the right border of the syringomycin gene cluster of Pseudomonas syringae pv. syringae. Mol Plant Microbe Interact. 2002;15:43-53 pubmed publisher
    ..These results demonstrate that salA is located upstream of syrF in the regulatory hierarchy controlling syringomycin production and virulence in P. syringae pv. syringae...
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    ..The secretion of two of these putative effectors was shown to be type III--dependent. Effectors showed high interstrain variation, supporting a role for some effectors in adaptation to different hosts...
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    ..The knowledge of the location of the Cu(II) in the protein is important for the copper transfer mechanism...
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    ..Therefore phase variation not only plays a role in escaping animal defense but it also appears to play a much broader and vital role in the ecology of bacteria producing exoenzymes, antibiotics, and other secondary metabolites...
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    ..The structure and arrangement of the modules lead to the formulation of a model explaining the synthesis of the tripeptide, including the formation of the two nonproteinogenic amino acids in the ring structure of syringolin A...
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    ..syringae pv. phaseolicola. The low-abundance hrcU mRNA had a half-life of 16.5 min, whereas other transcripts had half-lives between 3 and 8 min...
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    ..In contrast, the most efficient substrates for PcpS are CPs from primary metabolism. These results indicate phosphopantetheinyl transferases from different Pseudomonas sp. may vary significantly in their enzymatic properties...
  75. Zhang L, Koay M, Maher M, Xiao Z, Wedd A. Intermolecular transfer of copper ions from the CopC protein of Pseudomonas syringae. Crystal structures of fully loaded Cu(I)Cu(II) forms. J Am Chem Soc. 2006;128:5834-50 pubmed publisher
    ..CopC has the potential to interact directly with each of the four cop proteins coexpressed to the periplasm...
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    ..However, this is the first example of a bacterial pathogen exploiting a J domain protein to promote pathogenesis through alterations of chloroplast structure and function...
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    ..Upstream of mtlE, a putative promoter/operator region was identified by promoter probe studies which was active in P. fluorescens but not in E. coli...
  79. Hettwer U, Jaeckel F, Boch J, Meyer M, Rudolph K, Ullrich M. Cloning, nucleotide sequence, and expression in Escherichia coli of levansucrase genes from the plant pathogens Pseudomonas syringae pv. glycinea and P. syringae pv. phaseolicola. Appl Environ Microbiol. 1998;64:3180-7 pubmed
    ..coli. A PCR screening with primers derived from lsc of P. syringae pv. glycinea PG4180 allowed the detection of this gene in a number of related bacteria...
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    ..The present structure represents a link between copper-trafficking proteins and cupredoxins. Within a structural and genomic analysis, the role of CopC in copper trafficking is discussed...
  81. Arnesano F, Banci L, Bertini I, Mangani S, Thompsett A. A redox switch in CopC: an intriguing copper trafficking protein that binds copper(I) and copper(II) at different sites. Proc Natl Acad Sci U S A. 2003;100:3814-9 pubmed publisher
    ..CopC and CopA are coded in the same operon, responsible for copper resistance. These peculiar and novel properties of CopC are discussed with respect to their relevance for copper homeostasis...
  82. Pozidis C, Chalkiadaki A, Gomez Serrano A, Stahlberg H, Brown I, Tampakaki A, et al. Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization. J Biol Chem. 2003;278:25816-24 pubmed publisher
    ..We propose that TTS ATPases catalyze protein translocation as activated homo-oligomers at the plasma membrane...
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    ..syringae strains and that it evolved independently of the hrp pathogenicity island central conserved region, most likely through integron-like assembly of transposed gene cassettes...
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    ..We suggest that this interaction serves to further regulate activation of disease resistance signaling following recognition of P. syringae DC3000-AvrRpt2 by Arabidopsis...
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    ..Additionally, we illustrate the utility of this recombineering system to make targeted gene disruptions in the P. syringae chromosome...
  86. Liao C, McCallus D, Wells J, Tzean S, Kang G. The repB gene required for production of extracellular enzymes and fluorescent siderophores in Pseudomonas viridiflava is an analog of the gacA gene of Pseudomonas syringae. Can J Microbiol. 1996;42:177-82 pubmed
    ..fluorescens, and P. syringae included in the study. The data presented here and earlier indicate that the repA/repB gene regulatory system of P. viridiflava is analogous to the lemA/gacA system of P. syringae and P. fluorescens...
  87. Kannan K, Janiyani K, Shivaji S, Ray M. Histidine utilisation operon (hut) is upregulated at low temperature in the antarctic psychrotrophic bacterium Pseudomonas syringae. FEMS Microbiol Lett. 1998;161:7-14 pubmed
    ..syringae, P. fluorescens and P. putida also suggested that the hut operon is expressed at an elevated level at low temperature...
  88. De Vos D, Bouton C, Sarniguet A, de Vos P, Vauterin M, Cornelis P. Sequence diversity of the oprI gene, coding for major outer membrane lipoprotein I, among rRNA group I pseudomonads. J Bacteriol. 1998;180:6551-6 pubmed
    ..All other rRNA group I pseudomonads clustered in a manner that was in agreement with other studies, showing that the oprI gene can be useful as a complementary phylogenetic marker for classification of rRNA group I pseudomonads...
  89. Vermeij P, Wietek C, Kahnert A, W est T, Kertesz M. Genetic organization of sulphur-controlled aryl desulphonation in Pseudomonas putida S-313. Mol Microbiol. 1999;32:913-26 pubmed
    ..AsfR was a negative regulator of asfABC expression, and toluenesulphonate induced expression of these genes indirectly by reducing the expression of the asfR gene...
  90. Blumer C, Heeb S, Pessi G, Haas D. Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proc Natl Acad Sci U S A. 1999;96:14073-8 pubmed
    ..Mutational inactivation of the chromosomal rsmA gene partially suppressed a gacS defect. Thus, a central, GacA-dependent switch from primary to secondary metabolism may operate at the level of translation...
  91. Idei A, Kawai E, Akatsuka H, Omori K. Cloning and characterization of the Pseudomonas fluorescens ATP-binding cassette exporter, HasDEF, for the heme acquisition protein HasA. J Bacteriol. 1999;181:7545-51 pubmed
    ..fluorescens and S. marcescens sequences were produced and tested for secretion through the Has exporters. The C-terminal region of HasA was shown to be involved in the secretion specificity of the P. fluorescens Has exporter...
  92. Soutourina O, Semenova E, Parfenova V, Danchin A, Bertin P. Control of bacterial motility by environmental factors in polarly flagellated and peritrichous bacteria isolated from Lake Baikal. Appl Environ Microbiol. 2001;67:3852-9 pubmed
    ..Thus, striking similarities observed in the two organisms suggest that these processes have evolved toward a similar regulatory mechanism in polarly flagellated and laterally flagellated (peritrichous) bacteria...