Pseudomonas putida KT2440


Alias: Pseudomonas putida (strain KT2440), Pseudomonas putida str. KT2440

Top Publications

  1. Duque E, Segura A, Mosqueda G, Ramos J. Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida. Mol Microbiol. 2001;39:1100-6 pubmed
    ..The second ttgDEF efflux pump was expressed at low levels in this mutant strain, suggesting that the Lrp-like protein is a global regulatory protein involved in the solvent-tolerant response of this strain. ..
  2. Dekkers L, Bloemendaal C, de Weger L, Wijffelman C, Spaink H, Lugtenberg B. A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365. Mol Plant Microbe Interact. 1998;11:45-56 pubmed publisher
    ..We conclude that colonization is an active process in which an environmental stimulus, through this two-component system, activates a so far unknown trait that is crucial for colonization...
  3. Rojas A, Duque E, Mosqueda G, Golden G, Hurtado A, Ramos J, et al. Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E. J Bacteriol. 2001;183:3967-73 pubmed
    ..The triple mutant was hypersensitive to toluene, as shown by its inability to grow with toluene supplied via the vapor phase. ..
  4. Teran W, Felipe A, Segura A, Rojas A, Ramos J, Gallegos M. Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR. Antimicrob Agents Chemother. 2003;47:3067-72 pubmed
    ..This is the first case in which the expression of a drug transporter of the resistance-nodulation-division family has been shown to be regulated directly by antibiotics. ..
  5. Hõrak R, Ilves H, Pruunsild P, Kuljus M, Kivisaar M. The ColR-ColS two-component signal transduction system is involved in regulation of Tn4652 transposition in Pseudomonas putida under starvation conditions. Mol Microbiol. 2004;54:795-807 pubmed
    ..To our knowledge, Tn4652 is the first bacterial transposon regulated by a two-component system. This finding indicates that transpositional activity can respond to signals sensed and processed by the host. ..
  6. Teran W, Krell T, Ramos J, Gallegos M. Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem. 2006;281:7102-9 pubmed
    ..This provides important insight into the molecular mechanism of effector-mediated derepression. ..
  7. Lieder S, Jahn M, Koepff J, Müller S, Takors R. Environmental stress speeds up DNA replication in Pseudomonas putida in chemostat cultivations. Biotechnol J. 2016;11:155-63 pubmed publisher
    ..Applying continuous steady state cultivations (chemostats), the DNA content of a Pseudomonas putida KT2440 population was quantified with flow cytometry at distinct growth rates...
  8. Jeon E, Song J, Cha H, Lee S, Lee J, Park J. Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in Escherichia coli cell membrane. J Biotechnol. 2018;281:161-167 pubmed publisher
    ..coli expressing the ADH of M. luteus and a Baeyer-Villiger monooxygenase of Pseudomonas putida KT2440 was 2-fold increased to 40 U/g dry cells with expression of FadL to an appropriate level...
  9. Yu H, Tang H, Li Y, Xu P. Molybdenum-containing nicotine hydroxylase genes in a nicotine degradation pathway that is a variant of the pyridine and pyrrolidine pathways. Appl Environ Microbiol. 2015;81:8330-8 pubmed publisher
    ..genes were heterologously expressed in the non-nicotine-degrading strains Escherichia coli DH5α and Pseudomonas putida KT2440; only the Pseudomonas strain acquired the ability to degrade nicotine...

More Information

Publications319 found, 100 shown here

  1. Behrens B, Engelen J, Tiso T, Blank L, Hayen H. Characterization of rhamnolipids by liquid chromatography/mass spectrometry after solid-phase extraction. Anal Bioanal Chem. 2016;408:2505-14 pubmed publisher
    ..Solid-phase extraction exhibited higher yields and reproducibility as well as lower experimental effort. ..
  2. Rand J, Pisithkul T, Clark R, Thiede J, Mehrer C, Agnew D, et al. A metabolic pathway for catabolizing levulinic acid in bacteria. Nat Microbiol. 2017;2:1624-1634 pubmed publisher
    ..we report the identification and characterization of a seven-gene operon that enables LA catabolism in Pseudomonas putida KT2440. When the pathway was reconstituted with purified proteins, we observed the formation of four acyl-CoA ..
  3. Nazmi A, Muthu M, Lloyd Jones G. Manipulating intradiol dioxygenases by C-terminus truncation. Enzyme Microb Technol. 2019;125:21-28 pubmed publisher
    ..were selected and were originally described from the model organisms; Burkholderia xenovorans LB400, Pseudomonas putida KT2440 and Acinetobacter baylyi ADP1...
  4. Wittgens A, Santiago Schuebel B, Henkel M, Tiso T, Blank L, Hausmann R, et al. Heterologous production of long-chain rhamnolipids from Burkholderia glumae in Pseudomonas putida-a step forward to tailor-made rhamnolipids. Appl Microbiol Biotechnol. 2018;102:1229-1239 pubmed publisher
    ..for rhamnolipid biosynthesis in Burkholderia glumae were heterologously expressed in the non-pathogenic Pseudomonas putida KT2440. Our results show that long-chain rhamnolipids from Burkholderia spec. can be produced in P. putida...
  5. Burmeister A, Hilgers F, Langner A, Westerwalbesloh C, Kerkhoff Y, Tenhaef N, et al. A microfluidic co-cultivation platform to investigate microbial interactions at defined microenvironments. Lab Chip. 2018;19:98-110 pubmed publisher
    ..As a second example we investigated bacterial conjugation between Escherichia coli S17-1 and Pseudomonas putida KT2440 cells...
  6. Dong W, Chen Q, Hou Y, Li S, Zhuang K, Huang F, et al. Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA. Appl Environ Microbiol. 2015;81:8254-64 pubmed publisher
    ..Complementation with meaBA in MEA3-1Mut and heterologous expression in Pseudomonas putida strain KT2440 resulted in the production of an active MEHQ monooxygenase. ..
  7. Karmann S, Follonier S, Bassas Galia M, Panke S, Zinn M. Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a BODIPY 493/503-SYTO 62 double-staining. J Microbiol Methods. 2016;131:166-171 pubmed publisher
    ..Finally, fed-batch experiments with Pseudomonas putida KT2440 and Rhodospirillum rubrum S1 revealed that the method was robust and independent of the strain and type ..
  8. Baek A, Jeon E, Lee S, Park J. Expression levels of chaperones influence biotransformation activity of recombinant Escherichia coli expressing Micrococcus luteus alcohol dehydrogenase and Pseudomonas putida Baeyer-Villiger monooxygenase. Biotechnol Bioeng. 2015;112:889-95 pubmed publisher
    ..activity of recombinant Escherichia coli expressing Micrococcus luteus alcohol dehydrogenase and the Pseudomonas putida KT2440 Baeyer-Villiger monooxygenase improved significantly with co-expression of γ-prefoldin or recombinant ..
  9. Worrich A, König S, Miltner A, Banitz T, Centler F, Frank K, et al. Mycelium-Like Networks Increase Bacterial Dispersal, Growth, and Biodegradation in a Model Ecosystem at Various Water Potentials. Appl Environ Microbiol. 2016;82:2902-2908 pubmed publisher
    ..approach, we used a green fluorescent protein (GFP)-tagged derivative of the soil bacterium Pseudomonas putida KT2440 as a model organism and sodium benzoate as a representative of polar aromatic contaminants...
  10. Johnson C, Beckham G. Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin. Metab Eng. 2015;28:240-7 pubmed publisher
    ..We demonstrate that replacement of the catechol ortho degradation pathway endogenous to Pseudomonas putida KT2440 with an exogenous meta-cleavage pathway from P...
  11. Hiroe A, Watanabe S, Kobayashi M, Nomura C, Tsuge T. Increased synthesis of poly(3-hydroxydodecanoate) by random mutagenesis of polyhydroxyalkanoate synthase. Appl Microbiol Biotechnol. 2018;102:7927-7934 pubmed publisher
    ..PHA synthase was modified through random mutagenesis of a region of the PHA synthase 1 gene from Pseudomonas putida KT2440 (phaC1Pp)...
  12. Singh B, Röhm K. A new subfamily of bacterial glutamate/aspartate receptors. Biol Chem. 2008;389:33-6 pubmed the solute-binding protein AatJ that is required for glutamate uptake by the AatJMQP transporter in Pseudomonas putida KT2440. A homology model of the AatJ-glutamate complex was constructed using the E...
  13. Martínez García E, Aparicio T, de Lorenzo V, Nikel P. Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors. Methods Mol Biol. 2017;1498:273-293 pubmed
    ..of these tailored mini-transposon vectors, which could also be used for chromosomal engineering of a wide variety of Gram-negative microorganisms, is demonstrated in the platform environmental bacterium Pseudomonas putida KT2440.
  14. Currie F, Broadhurst D, Dunn W, Sellick C, Goodacre R. Metabolomics reveals the physiological response of Pseudomonas putida KT2440 (UWC1) after pharmaceutical exposure. Mol Biosyst. 2016;12:1367-77 pubmed publisher
    ..In this study we attempt to measure the effects of exposing cultures of Pseudomonas putida KT2440 (UWC1) to six pharmaceuticals by looking at differences in metabolite levels...
  15. Eberlein C, Starke S, Doncel Á, Scarabotti F, Heipieper H. Quantification of outer membrane vesicles: a potential tool to compare response in Pseudomonas putida KT2440 to stress caused by alkanols. Appl Microbiol Biotechnol. 2019;103:4193-4201 pubmed publisher
  16. Wu Z, Chen Z, Gao X, Li J, Shang G. Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing. Appl Microbiol Biotechnol. 2019;103:2783-2795 pubmed publisher
    b>Pseudomonas putida KT2440 is a Gram-negative, biosafety strain that plays important roles in environmental and biotechnological applications...
  17. Kohlstedt M, Wittmann C. GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas aeruginosa PAO1. Metab Eng. 2019;54:35-53 pubmed publisher
    ..setup created 534 mass isotopomers and enabled high-resolution flux analysis of the cell factory Pseudomonas putida KT2440 and the human pathogen P. aeruginosa PAO1...
  18. Lu J, Wang Y, Li J, Mao L, Nguyen S, Duarte T, et al. Triclosan at environmentally relevant concentrations promotes horizontal transfer of multidrug resistance genes within and across bacterial genera. Environ Int. 2018;121:1217-1226 pubmed publisher
    ..coli K-12 MG1655 or Pseudomonas putida KT2440 were the intra- and inter-genera recipients, respectively...
  19. Tarazona N, Maestro B, Revelles O, Sanz J, Prieto M. Role of leucine zipper-like motifs in the oligomerization of Pseudomonas putida phasins. Biochim Biophys Acta Gen Subj. 2018;1863:362-370 pubmed publisher
    ..used to characterize the coiled-coil motifs of phasins PhaF and PhaI -produced by the model bacterium Pseudomonas putida KT2440-...
  20. Tan C, Zhang X, Zhu Z, Xu M, Yang T, Osire T, et al. Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440. Microb Cell Fact. 2019;18:12 pubmed publisher
    ..9 h compared to only 3.2 h of the wild type SMO. Moreover, overexpression of SMO in Pseudomonas putida KT2440 with NADH regeneration was carried out in order to improve biotransformation efficiency for epoxide ..
  21. Pérez de Luque A, Tille S, Johnson I, Pascual Pardo D, Ton J, Cameron D. The interactive effects of arbuscular mycorrhiza and plant growth-promoting rhizobacteria synergistically enhance host plant defences against pathogens. Sci Rep. 2017;7:16409 pubmed publisher
    ..were (co)inoculated with the mycorrhizal fungus Rhizophagus irregularis and the rhizobacterial strain Pseudomonas putida KT2440. The cultivar with high mycorrhizal compatibility supported higher levels of rhizobacterial colonization ..
  22. Gao J, Ramsay J, Ramsay B. Fed-batch production of poly-3-hydroxydecanoate from decanoic acid. J Biotechnol. 2016;218:102-7 pubmed publisher
    ..A fed-batch fermentation process was developed for the production of MCL-PHA from decanoic acid using Pseudomonas putida KT2440. Decanoic acid was kept in liquid form by heating or by mixing with acetic acid to prevent ..
  23. Borrero de Acuña J, Hidalgo Dumont C, Pacheco N, Cabrera A, Poblete Castro I. A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production. Sci Rep. 2017;7:4373 pubmed publisher
    ..inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in ..
  24. Mozejko Ciesielska J, Pokoj T, Ciesielski S. Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress. J Ind Microbiol Biotechnol. 2018;45:433-446 pubmed publisher
    ..In this study, we used transcriptome analysis of Pseudomonas putida KT2440 to gain a genome-wide view of the mechanisms of environmental-friendly biopolymers accumulation under ..
  25. Vives Peris V, Molina L, Segura A, Gomez Cadenas A, Pérez Clemente R. Root exudates from citrus plants subjected to abiotic stress conditions have a positive effect on rhizobacteria. J Plant Physiol. 2018;228:208-217 pubmed publisher, the effect of root exudates obtained from in vitro cultured citrus plants on two rhizobacteria (Pseudomonas putida KT2440 and Novosphingobium sp. HR1a) was evaluated...
  26. Heinrich D, Raberg M, Fricke P, Kenny S, Morales Gamez L, Babu R, et al. Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum. Appl Environ Microbiol. 2016;82:6132-6140 pubmed
    ..For this, genes from Pseudomonas putida KT2440 coding for a 3-hydroxyacyl acyl carrier protein (ACP) thioesterase (phaG), a medium-chain-length (MCL) ..
  27. Xiao Y, Liu H, Nie H, Xie S, Luo X, Chen W, et al. Expression of the phosphodiesterase BifA facilitating swimming motility is partly controlled by FliA in Pseudomonas putida KT2440. Microbiologyopen. 2017;6: pubmed publisher
    ..Our results suggest that FliA acts as a negative regulator to modulate the c-di-GMP level via controlling transcription of bifA to facilitate swimming motility. ..
  28. Liu H, Yan H, Xiao Y, Nie H, Huang Q, Chen W. The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440. Microbiol Res. 2019;218:1-11 pubmed publisher
    In Pseudomonas putida KT2440, the exopolysaccharide Pea is associated with biofilm stability and pellicle formation; however, little is known about its regulatory pathway...
  29. Martín Mora D, Reyes Darias J, Ortega Ã, Corral Lugo A, Matilla M, Krell T. McpQ is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes. Environ Microbiol. 2016;18:3284-3295 pubmed publisher
    ..Therefore, chemotaxis to compound families by the concerted action of broad and narrow range receptors may represent a general mechanism. ..
  30. Xiao Y, Zhu W, Liu H, Nie H, Chen W, Huang Q. FinR Regulates Expression of nicC and nicX Operons, Involved in Nicotinic Acid Degradation in Pseudomonas putida KT2440. Appl Environ Microbiol. 2018;84: pubmed publisher
    ..The work deepened our knowledge of FinR function and nicotinic acid degradation in P. putida. ..
  31. Espeso D, Martinez Garcia E, Carpio A, De Lorenzo V. Dynamics of Pseudomonas putida biofilms in an upscale experimental framework. J Ind Microbiol Biotechnol. 2018;45:899-911 pubmed publisher
    ..While hydrodynamics could be used to control material features of biofilms of the platform strain Pseudomonas putida KT2440 there is a dearth of experimental data on surface-associated growth behavior in such settings...
  32. Nikel P, Romero Campero F, Zeidman J, Goñi Moreno Ã, de Lorenzo V. The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor. MBio. 2015;6: pubmed publisher
    The growth of the soil bacterium Pseudomonas putida KT2440 on glycerol as the sole carbon source is characterized by a prolonged lag phase, not observed with other carbon substrates...
  33. Zhang M, Gao C, Guo X, Guo S, Kang Z, Xiao D, et al. Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving L-2-hydroxyglutarate. Nat Commun. 2018;9:2114 pubmed publisher
    ..Here, we discover that Pseudomonas putida KT2440 has an additional glutarate catabolic pathway involving L-2-hydroxyglutarate (L-2-HG), an abnormal ..
  34. Blanco Romero E, Redondo Nieto M, Martínez Granero F, Garrido Sanz D, Ramos Gonzalez M, Martin M, et al. Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440. Sci Rep. 2018;8:13145 pubmed publisher
    ..putatively regulated by the transcriptional regulatory protein FleQ in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440. This protein was previously identified as a master regulator of flagella and biofilm formation in both ..
  35. Granja Travez R, Bugg T. Characterization of multicopper oxidase CopA from Pseudomonas putida KT2440 and Pseudomonas fluorescens Pf-5: Involvement in bacterial lignin oxidation. Arch Biochem Biophys. 2018;660:97-107 pubmed publisher
    ..A double gene deletion of copA-I and copA-II genes in Pseudomonas putida KT2440 was constructed, and this mutant showed diminished growth capability on different small aromatic ..
  36. Yun C, Takahashi Y, Shintani M, Takeda T, Suzuki Minakuchi C, Okada K, et al. MvaT Family Proteins Encoded on IncP-7 Plasmid pCAR1 and the Host Chromosome Regulate the Host Transcriptome Cooperatively but Differently. Appl Environ Microbiol. 2016;82:832-42 pubmed publisher
    ..The IncP-7 conjugative plasmid pCAR1 carries an mvaT-homologous gene, pmr. In Pseudomonas putida KT2440 bearing pCAR1, pmr and the chromosomally carried homologous genes, turA and turB, are transcribed at ..
  37. Belda E, van Heck R, José Lopez Sanchez M, Cruveiller S, Barbe V, Fraser C, et al. The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis. Environ Microbiol. 2016;18:3403-3424 pubmed publisher
    By the time the complete genome sequence of the soil bacterium Pseudomonas putida KT2440 was published in 2002 (Nelson et al...
  38. Wigneswaran V, Nielsen K, Sternberg C, Jensen P, Folkesson A, Jelsbak L. Biofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain Pseudomonas putida KT2440. Microb Cell Fact. 2016;15:181 pubmed
    ..of biosurfactants was investigated using rhamnolipids as the model compound in biofilm encased Pseudomonas putida KT2440. The rhlAB operon from P. aeruginosa was introduced into P. putida to produce mono-rhamnolipids...
  39. Thuptimdang P, Limpiyakorn T, McEvoy J, Prüß B, Khan E. Effect of silver nanoparticles on Pseudomonas putida biofilms at different stages of maturity. J Hazard Mater. 2015;290:127-33 pubmed publisher
    This study determined the effect of silver nanoparticles (AgNPs) on Pseudomonas putida KT2440 biofilms at different stages of maturity...
  40. Ravi K, García Hidalgo J, Gorwa Grauslund M, Liden G. Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost. Appl Microbiol Biotechnol. 2017;101:5059-5070 pubmed publisher
    ..and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection...
  41. Hanko E, Minton N, Malys N. Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator. Sci Rep. 2017;7:1724 pubmed publisher
    ..characterise 3-HP-inducible promoters and their corresponding LysR-type transcriptional regulators from Pseudomonas putida KT2440. A newly-developed modular reporter system proved possible to demonstrate that PpMmsR/P mmsA ..
  42. Cho Y, Kim S, Kim J, Lee D, Park K, Park Y. Effect of PelB signal sequences on Pfe1 expression and ω-hydroxyundec-9-enoic acid biotransformation in recombinant Escherichia coli. Appl Microbiol Biotechnol. 2018;102:7407-7416 pubmed publisher alcohol dehydrogenase (Adh) from Micrococcus luteus, a Baeyer-Villiger monooxygenase (BVMO) from Pseudomonas putida KT2440 and an esterase (Pfe1) from Pseudomonas fluorescens SIK WI were overexpressed in Escherichia coli BL21(..
  43. Prieto A, Escapa I, Martínez V, Dinjaski N, Herencias C, de la Peña F, et al. A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida. Environ Microbiol. 2016;18:341-57 pubmed publisher
    ..between bacterial physiology, PHA metabolism and other cell functions in model bacteria such as Pseudomonas putida KT2440. PHA granules or carbonosomes are supramolecular complexes of biopolyester and proteins that are ..
  44. Hokamura A, Wakida I, Miyahara Y, Tsuge T, Shiratsuchi H, Tanaka K, et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose. J Biosci Bioeng. 2015;120:305-10 pubmed publisher
    ..transferase, actually functions as a 3-hydroxyacyl-ACP thioesterase, and the product of PP0763 gene from Pseudomonas putida KT2440 has a (R)-3-hydroxyacyl (3HA)-CoA ligase activity (Wang et al., Appl. Environ. Microbiol...
  45. Ibrahim S, Crack J, Rolfe M, Borrero de Acuña J, Thomson A, Le Brun N, et al. Three Pseudomonas putida FNR Family Proteins with Different Sensitivities to O2. J Biol Chem. 2015;290:16812-23 pubmed publisher
    ..Here, three FNR proteins (ANR, PP_3233, and PP_3287) from a single bacterial species, Pseudomonas putida KT2440, have been analyzed...
  46. Benedetti I, de Lorenzo V, Nikel P. Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes. Metab Eng. 2016;33:109-118 pubmed publisher
    ..The transition between planktonic and biofilm lifestyles of the platform strain Pseudomonas putida KT2440 is ruled by a regulatory network controlling the levels of the trigger signal cyclic di-GMP (c-di-GMP)...
  47. Corral Lugo A, de la Torre J, Matilla M, Fernández M, Morel B, Espinosa Urgel M, et al. Assessment of the contribution of chemoreceptor-based signalling to biofilm formation. Environ Microbiol. 2016;18:3355-3372 pubmed publisher
    ..Data agree with other reports showing that polyamines are signal molecules involved in the regulation of bacteria-plant communication and biofilm formation. ..
  48. Beckers V, Poblete Castro I, Tomasch J, Wittmann C. Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol. Microb Cell Fact. 2016;15:73 pubmed publisher
    ..The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of ..
  49. Huertas Rosales Ã, Ramos González M, Espinosa Urgel M. Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida. Appl Environ Microbiol. 2016;82:5673-86 pubmed publisher
    In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P...
  50. Aparicio T, De Lorenzo V, Martinez Garcia E. CRISPR/Cas9-based counterselection boosts recombineering efficiency in Pseudomonas putida. Biotechnol J. 2017;: pubmed publisher DNA recombineering techniques has greatly eased genetic design of the platform strain Pseudomonas putida KT2440, available methods still produce the desired modifications/deletions at low frequencies...
  51. Suzuki Minakuchi C, Hirotani R, Shintani M, Takeda T, Takahashi Y, Matsui K, et al. Effects of three different nucleoid-associated proteins encoded on IncP-7 plasmid pCAR1 on host Pseudomonas putida KT2440. Appl Environ Microbiol. 2015;81:2869-80 pubmed publisher
    ..In this study, the effects of single or double disruption of pmr, pnd, and phu were assessed in host Pseudomonas putida KT2440. When pmr and pnd or pmr and phu were simultaneously disrupted, both the segregational stability and the ..
  52. Mazurkewich S, Brott A, Kimber M, Seah S. Structural and Kinetic Characterization of the 4-Carboxy-2-hydroxymuconate Hydratase from the Gallate and Protocatechuate 4,5-Cleavage Pathways of Pseudomonas putida KT2440. J Biol Chem. 2016;291:7669-86 pubmed publisher
    ..A comparison with the PIG-L deacetylases led to a proposed mechanism for GalB wherein Glu-48 positions and activates the metal-ligated water for the hydration reaction and His-164 acts as a catalytic acid. ..
  53. An R, Moe L. Regulation of Pyrroloquinoline Quinone-Dependent Glucose Dehydrogenase Activity in the Model Rhizosphere-Dwelling Bacterium Pseudomonas putida KT2440. Appl Environ Microbiol. 2016;82:4955-64 pubmed publisher
    ..Here we use the model rhizosphere-dwelling bacterium Pseudomonas putida KT2440 to explore GDH activity and PQQ synthesis, as well as gene expression of the GDH-encoding gene (gcd) and ..
  54. Patil M, Patel G, Surywanshi B, Shaikh N, Garg P, Chisti Y, et al. Disruption of Pseudomonas putida by high pressure homogenization: a comparison of the predictive capacity of three process models for the efficient release of arginine deiminase. AMB Express. 2016;6:84 pubmed publisher
    Disruption of Pseudomonas putida KT2440 by high-pressure homogenization in a French press is discussed for the release of arginine deiminase (ADI)...
  55. Obeng E, Brossette T, Ongkudon C, Budiman C, Maas R, Jose J. The workability of Escherichia coli BL21 (DE3) and Pseudomonas putida KT2440 expression platforms with autodisplayed cellulases: a comparison. Appl Microbiol Biotechnol. 2018;102:4829-4841 pubmed publisher
    This article comparatively reports the workability of Escherichia coli BL21(DE3) and Pseudomonas putida KT2440 cell factories for the expression of three model autodisplayed cellulases (i.e...
  56. Gupta I, Anderson A, Rai M. Toxicity of fungal-generated silver nanoparticles to soil-inhabiting Pseudomonas putida KT2440, a rhizospheric bacterium responsible for plant protection and bioremediation. J Hazard Mater. 2015;286:48-54 pubmed publisher
    ..Silver nanoparticles obtained herein were found to exert the hazardous effect at the concentration of 0.4 μg/ml, which warrants further detailed investigations concerning toxicity. ..
  57. Sun J, Wang Q, Jiang Y, Wen Z, Yang L, Wu J, et al. Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microb Cell Fact. 2018;17:41 pubmed publisher
    The soil bacterium Pseudomonas putida KT2440 is a "generally recognized as safe"-certified strain with robust property and versatile metabolism...
  58. Martínez García E, Aparicio T, de Lorenzo V, Nikel P. New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014;2:46 pubmed publisher
    ..After validating the pBAMD vectors in the environmental bacterium Pseudomonas putida KT2440, their use was also illustrated by inserting the entire poly(3-hydroxybutyrate) (PHB) synthesis pathway ..
  59. Nikel P, Chavarría M, Fuhrer T, Sauer U, de Lorenzo V. Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways. J Biol Chem. 2015;290:25920-32 pubmed publisher
    The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the Entner-Doudoroff (ED) route...
  60. Equar M, Tani Y, Mihara H. Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. J Nutr Sci Vitaminol (Tokyo). 2015;61:506-10 pubmed publisher
    ..We have purified and characterized ThiO from Pseudomonas putida KT2440. It has a monomeric structure that is distinct from the homotetrameric ThiOs from Bacillus subtilis and ..
  61. Chen Z, Ling W, Shang G. Recombineering and I-SceI-mediated Pseudomonas putida KT2440 scarless gene deletion. FEMS Microbiol Lett. 2016;: pubmed
    b>Pseudomonas putida KT2440 is a saprophytic, generally recognized as safe microorganism that plays important roles in the biodegradation and production of value-added chemicals. Chromosomal gene deletion of P...
  62. Beuker J, Barth T, Steier A, Wittgens A, Rosenau F, Henkel M, et al. High titer heterologous rhamnolipid production. AMB Express. 2016;6:124 pubmed publisher
    Heterologous mono-rhamnolipid production by Pseudomonas putida KT2440 pSynPro8oT_rhlAB using glucose as the single carbon source was characterized in fed-batch bioreactor cultivations...
  63. Elmore J, Furches A, Wolff G, Gorday K, Guss A. Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440. Metab Eng Commun. 2017;5:1-8 pubmed publisher
    ..This genome integration and reporter systems are extensible for testing other genetic parts, such as examining terminator strength, and will allow rapid integration of heterologous pathways for metabolic engineering...
  64. Qiu Y, Zhang J, Li B, Wen X, Liang P, Huang X. A novel microfluidic system enables visualization and analysis of antibiotic resistance gene transfer to activated sludge bacteria in biofilm. Sci Total Environ. 2018;642:582-590 pubmed publisher
    ..g., activated sludge bacteria. The average plasmid transfer frequency per receptor (T/R) from RP4-hosted Pseudomonas putida KT2440 to activated sludge bacteria was quantified to be 2...
  65. Vives Peris V, Gomez Cadenas A, Pérez Clemente R. Salt stress alleviation in citrus plants by plant growth-promoting rhizobacteria Pseudomonas putida and Novosphingobium sp. Plant Cell Rep. 2018;37:1557-1569 pubmed publisher
    ..In this work, Citrus macrophylla (alemow) plants inoculated with the rhizobacteria Pseudomonas putida KT2440 or Novosphingobium sp. HR1a were subjected to salt stress for 30 days...
  66. Borodin A, Danilkovich A, Allikmets R. [Nucleotide sequence of the rplL gene coding for ribosomal protein L7/L12 of Pseudomonas putida]. Bioorg Khim. 1989;15:560-1 pubmed
    ..Although Asp55 residue in L7/L12 was previously shown to be conservative in ten different organisms, the Pseudomonas putida L7/L12 proved to contain Asn55, thus showing that Asp55 is not invariant. ..
  67. Lüneberg E, Muller D, Steinmetz I, Frosch M. Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species. FEMS Microbiol Lett. 1997;154:131-7 pubmed
    ..aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the gamma-subclass of the Proteobacteria. ..
  68. Baune M, Qi Y, Scholz K, Volmer D, Hayen H. Structural characterization of pyoverdines produced by Pseudomonas putida KT2440 and Pseudomonas taiwanensis VLB120. Biometals. 2017;30:589-597 pubmed publisher
    ..Peptide sequencing by MS/MS provided the sequence Orn-Asp-OHAsn-Thr-AcOHOrn-Ser-cOHOrn. Of particular interest is the presence of OHAsn, which has not been reported as PVD constituent before. ..
  69. Jiang T, Guo X, Yan J, Zhang Y, Wang Y, Zhang M, et al. A Bacterial Multidomain NAD-Independent d-Lactate Dehydrogenase Utilizes Flavin Adenine Dinucleotide and Fe-S Clusters as Cofactors and Quinone as an Electron Acceptor for d-Lactate Oxidization. J Bacteriol. 2017;199: pubmed publisher
    ..A study of it will be helpful in understanding the detailed mechanisms underlying the lactate utilization processes. ..
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    The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P...
  71. H rak R, Kivisaar M. Expression of the transposase gene tnpA of Tn4652 is positively affected by integration host factor. J Bacteriol. 1998;180:2822-9 pubmed
    ..Also, an in vitro binding assay demonstrated that both ends of Tn4652 bind IHF from a cell lysate of E. coli...
  72. Wang Y, Zhang C, Gong T, Zuo Z, Zhao F, Fan X, et al. An upp-based markerless gene replacement method for genome reduction and metabolic pathway engineering in Pseudomonas mendocina NK-01 and Pseudomonas putida KT2440. J Microbiol Methods. 2015;113:27-33 pubmed publisher
    ..The upp-based counterselection system could be further adapted for P. mendocina NK-01 and P. putida KT2440 and used for genome reduction and metabolic pathway engineering. ..
  73. Franden M, Jayakody L, Li W, Wagner N, Cleveland N, Michener W, et al. Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization. Metab Eng. 2018;48:197-207 pubmed publisher
    ..b>Pseudomonas putida KT2440, which has been engineered for environmental remediation applications given its high toxicity tolerance ..
  74. Burns G, Sykes P, Hatter K, Sokatch J. Isolation of a third lipoamide dehydrogenase from Pseudomonas putida. J Bacteriol. 1989;171:665-8 pubmed
    ..These results suggest that LPD-3 is a lipoamide dehydrogenase associated with an unknown multienzyme complex which can replace LPD-Glc as the E3 component of pyruvate and 2-ketoglutarate dehydrogenases in lpdG mutants. ..
  75. Iwaki M, Kagamiyama H, Nozaki M. The primary structure of the beta-subunit of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Arch Biochem Biophys. 1981;210:210-23 pubmed
  76. Valentin H, Stuart E, Fuller R, Lenz R, Dennis D. Investigation of the function of proteins associated to polyhydroxyalkanoate inclusions in Pseudomonas putida BMO1. J Biotechnol. 1998;64:145-57 pubmed
    ..Gas-chromatographic analysis of the cellular dry mass revealed that the mutant strains accumulated 30-50% less PHA than the P. putida wild type...
  77. Dubern J, Lagendijk E, Lugtenberg B, Bloemberg G. The heat shock genes dnaK, dnaJ, and grpE are involved in regulation of putisolvin biosynthesis in Pseudomonas putida PCL1445. J Bacteriol. 2005;187:5967-76 pubmed publisher
    ..In addition, putisolvin biosynthesis of PCL1445 was found to be dependent on the GacA/GacS two-component signaling system. Expression analysis indicated that dnaK is positively regulated by GacA/GacS...
  78. Vrionis H, Daugulis A, Kropinski A. Identification and characterization of the AgmR regulator of Pseudomonas putida: role in alcohol utilization. Appl Microbiol Biotechnol. 2002;58:469-75 pubmed
    ..The successful elimination of alcohol utilization in the agmR mutant indicates control by AgmR on multiple pathways and presents a useful strain for biotechnological applications requiring alcohol non-utilizing microbial catalysts. ..
  79. Luo X, Yang Y, Ling W, Zhuang H, Li Q, Shang G. Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination. FEMS Microbiol Lett. 2016;363: pubmed publisher
    b>Pseudomonas putida KT2440 is a saprophytic, environmental microorganism that plays important roles in the biodegradation of environmental toxic compounds and production of polymers, chemicals and secondary metabolites...
  80. Wehrmann M, Berthelot C, Billard P, Klebensberger J. The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440. mSphere. 2018;3: pubmed publisher
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    ..The high sequence and structure conservation of the PutA RHH domain suggest interdomain interactions play an important role in the evolution of the protein. ..
  82. Wittgens A, Kovačić F, Müller M, Gerlitzki M, Santiago Schübel B, Hofmann D, et al. Novel insights into biosynthesis and uptake of rhamnolipids and their precursors. Appl Microbiol Biotechnol. 2017;101:2865-2878 pubmed publisher
    ..proteins involved in rhamnolipid biosynthesis the heterologous expression of rhl-genes in non-pathogenic Pseudomonas putida KT2440 strains was used in this study to circumvent the complex quorum sensing regulation in P. aeruginosa...
  83. Hirayama H, Takami H, Inoue A, Horikoshi K. Isolation and characterization of toluene-sensitive mutants from Pseudomonas putida IH-2000. FEMS Microbiol Lett. 1998;169:219-25 pubmed
    ..Our findings indicate that cyo is an important gene for toluene tolerance, although its role is still unclear...
  84. Bond C, Huang J, Hajduk R, Flick K, Heath P, Stoddard B. Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis. Acta Crystallogr D Biol Crystallogr. 2000;56:1359-66 pubmed
    ..The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution...
  85. Casey W, Nikodinovic Runic J, Fonseca Garcia P, Guzik M, McGrath J, Quinn J, et al. The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida?KT2440. Environ Microbiol Rep. 2013;5:740-6 pubmed publisher
    The primary enzyme involved in polyphosphate (polyP) synthesis, polyP kinase (ppk), has been deleted in Pseudomonas putida KT2440. This has resulted in a threefold to sixfold reduction in polyhydroxyalkanoate (PHA) accumulation compared ..
  86. McCarthy C, Woods R, Beacham I. Regulation of the aprX-lipA operon of Pseudomonas fluorescens B52: differential regulation of the proximal and distal genes, encoding protease and lipase, by ompR-envZ. FEMS Microbiol Lett. 2004;241:243-8 pubmed publisher
    ..Differences in the regulation of lipase and protease may be related to the proximal and distal locations of aprX and lipA within the operon...
  87. Kim H, Park S, Cho S, Yang J, Jeong K, Park J, et al. Bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant Corynebacterium glutamicum-based biocatalyst. J Ind Microbiol Biotechnol. 2017;44:1301-1311 pubmed publisher
    Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid...
  88. Kohler T, Alvarez J, Harayama S. Regulation of the rpoN, ORF102 and ORF154 genes in Pseudomonas putida. FEMS Microbiol Lett. 1994;115:177-84 pubmed
    ..putida was independent of the nitrogen status of the cell but was 5 times higher in the rpoN mutant than in the wild-type strain, suggesting that the expression of the rpoN gene in this organism is autoregulated. ..
  89. Kivistik P, Kivi R, Kivisaar M, H rak R. Identification of ColR binding consensus and prediction of regulon of ColRS two-component system. BMC Mol Biol. 2009;10:46 pubmed publisher
    ..putida. It is notable that many of ColR-regulated genes encode membrane-related products thus confirming the previously proposed role of ColRS system in regulation of membrane functionality...
  90. Tettmann B, Dötsch A, Armant O, Fjell C, Overhage J. Knockout of extracytoplasmic function sigma factor ECF-10 affects stress resistance and biofilm formation in Pseudomonas putida KT2440. Appl Environ Microbiol. 2014;80:4911-9 pubmed publisher
    ..putida KT2440. ..
  91. Simon O, Klebensberger J, Mükschel B, Klaiber I, Graf N, Altenbuchner J, et al. Analysis of the molecular response of Pseudomonas putida KT2440 to the next-generation biofuel n-butanol. J Proteomics. 2015;122:11-25 pubmed publisher
    ..proteome and phospholipid analysis to characterize the molecular response of the potential biocatalyst Pseudomonas putida KT2440 to the next-generation biofuel n-butanol...
  92. Herrera M, Duque E, Rodr guez Herva J, Fern ndez Escamilla A, Ramos J. Identification and characterization of the PhhR regulon in Pseudomonas putida. Environ Microbiol. 2010;12:1427-38 pubmed publisher
    ..These results place PhhR as an important regulator in the control of bacterial responses to aromatic amino acids...