Genomes and Genes
Pseudomonas aeruginosa PAO1
Alias: Pseudomonas aeruginosa str. PAO1, Pseudomonas aeruginosa PA01, Pseudomonas aeruginosa str. PA01
Publications143 found, 100 shown here
- O Callaghan J, Reen F, Adams C, Casey P, Gahan C, O Gara F. A novel host-responsive sensor mediates virulence and type III secretion during Pseudomonas aeruginosa-host cell interactions. Microbiology. 2012;158:1057-70 pubmed publisher..Through this signal transduction pathway, T3SS activation was mediated via the RsmAYZ regulatory cascade and involved the global anaerobic response regulator Anr. ..
- Maseda H, Uwate M, Nakae T. Transcriptional regulation of the mexEF-oprN multidrug efflux pump operon by MexT and an unidentified repressor in nfxC-type mutant of Pseudomonas aeruginosa. FEMS Microbiol Lett. 2010;311:36-43 pubmed publisher..Deletion of this region caused a sudden rise in MexEF-OprN production, suggesting that this region accommodates the binding of a putative repressor protein. ..
- Kong K, Aguila A, Schneper L, Mathee K. Pseudomonas aeruginosa ?-lactamase induction requires two permeases, AmpG and AmpP. BMC Microbiol. 2010;10:328 pubmed publisher..b>Pseudomonas aeruginosa PAO1 has two ampG paralogs, PA4218 (ampP) and PA4393 (ampG)...
- Abdou L, Chou H, Haas D, Lu C. Promoter recognition and activation by the global response regulator CbrB in Pseudomonas aeruginosa. J Bacteriol. 2011;193:2784-92 pubmed publisher..Based on these results and previously published data, a consensus CbrB recognition sequence is proposed. This sequence has similarity to the consensus NtrC recognition sequence, which is relevant for nitrogen control. ..
- Damron F, Owings J, Okkotsu Y, Varga J, Schurr J, Goldberg J, et al. Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN. J Bacteriol. 2012;194:1317-30 pubmed publisher..Together, these data strongly suggest that KinB regulates virulence factors important for the development of acute pneumonia and conversion to mucoidy. ..
- Aguirre Ramirez M, Medina G, González Valdez A, Grosso Becerra V, Soberón Chávez G. The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor ?S. Microbiology. 2012;158:908-16 pubmed publisher..One of these transcriptional start sites (P2) is responsible for most of its expression and is dependent on the stationary phase sigma factor ?(S) and on RhlR/C(4)-HSL through its binding to an atypical 'las box'. ..
- Bardoel B, Hartsink D, Vughs M, de Haas C, Van Strijp J, Van Kessel K. Identification of an immunomodulating metalloprotease of Pseudomonas aeruginosa (IMPa). Cell Microbiol. 2012;14:902-13 pubmed publisher..Next to PSGL-1, IMPa targets CD43 and CD44 that are also involved in leucocyte homing. These data indicate that IMPa prevents neutrophil extravasation and thereby protects P. aeruginosa from neutrophil attack. ..
- Balasubramanian D, Schneper L, Merighi M, Smith R, Narasimhan G, Lory S, et al. The regulatory repertoire of Pseudomonas aeruginosa AmpC ß-lactamase regulator AmpR includes virulence genes. PLoS ONE. 2012;7:e34067 pubmed publisher..Unraveling this complex regulatory circuit will provide a better understanding of the bacterial response to antibiotics and how the organism coordinately regulates a myriad of virulence factors in response to antibiotic exposure. ..
- Islam S, Fieldhouse R, Anderson E, Taylor V, Keates R, Ford R, et al. A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1. Mol Microbiol. 2012;84:1165-76 pubmed publisherHeteropolymeric B-band O-antigen (O-Ag) biosynthesis in Pseudomonas aeruginosa PAO1 follows the Wzy-dependent pathway, beginning with translocation of undecaprenyl pyrophosphate-linked anionic O-Ag subunits (O units) from the inner to ..
- Petrova O, Sauer K. PAS domain residues and prosthetic group involved in BdlA-dependent dispersion response by Pseudomonas aeruginosa biofilms. J Bacteriol. 2012;194:5817-28 pubmed publisher..The findings suggest that BdlA plays a role in intracellular sensing of dispersion-inducing conditions and together with DipA forms a regulatory network that modulates an intracellular cyclic d-GMP (c-di-GMP) pool to enable dispersion. ..
- Jin Y, Yang H, Qiao M, Jin S. MexT regulates the type III secretion system through MexS and PtrC in Pseudomonas aeruginosa. J Bacteriol. 2011;193:399-410 pubmed publisher..A model has been proposed for the MexS/MexT-mediated regulation of the T3SS, the MexEF efflux pump, and the production of elastase and pyocyanin. ..
- Schweizer H, Choi K. Pseudomonas aeruginosa aerobic fatty acid desaturase DesB is important for virulence factor production. Arch Microbiol. 2011;193:227-34 pubmed publisher..Taken together, these results demonstrate that DesB is not only a fatty acid desaturase but also a factor required for full virulence in Pseudomonas aeruginosa. DesB may thus constitute a novel drug target. ..
- Fito Boncompte L, Chapalain A, Bouffartigues E, Chaker H, Lesouhaitier O, Gicquel G, et al. Full virulence of Pseudomonas aeruginosa requires OprF. Infect Immun. 2011;79:1176-86 pubmed publisher..This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production, providing novel insights into virulence expression. ..
- Bartels K, Funken H, Knapp A, Brocker M, Bott M, Wilhelm S, et al. Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa. J Bacteriol. 2011;193:1107-13 pubmed publisher..Furthermore, we demonstrate that this glycosylation is required for the transport of LecB. ..
- Petrova O, Schurr J, Schurr M, Sauer K. The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA. Mol Microbiol. 2011;81:767-83 pubmed publisher..Therefore, we propose that BfmR regulates biofilm development by limiting bacteriophage-mediated lysis and thus, eDNA release, via PhdA. ..
- Somprasong N, Jittawuttipoka T, Duang Nkern J, Romsang A, Chaiyen P, Schweizer H, et al. Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. J Bacteriol. 2012;194:3904-12 pubmed publisherThe Pseudomonas aeruginosa PAO1 thiol peroxidase homolog (Tpx) belongs to a family of enzymes implicated in the removal of toxic peroxides...
- Bouffartigues E, Gicquel G, Bazire A, Bains M, Maillot O, Vieillard J, et al. Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced. J Bacteriol. 2012;194:4301-11 pubmed publisher..The lack of OprF itself increased oprF transcription. Since these conditions led to cell wall alterations, oprF transcription could be activated by signals triggered by perturbation of the cell envelope. ..
- Huangyutitham V, Güvener Z, Harwood C. Subcellular clustering of the phosphorylated WspR response regulator protein stimulates its diguanylate cyclase activity. MBio. 2013;4:e00242-13 pubmed publisher..We conclude that oligomer formation visualized as subcellular clusters is an additional mechanism by which the activities of response regulator-diguanylate cyclases can be regulated. ..
- Liao J, Schurr M, Sauer K. The MerR-like regulator BrlR confers biofilm tolerance by activating multidrug efflux pumps in Pseudomonas aeruginosa biofilms. J Bacteriol. 2013;195:3352-63 pubmed publisher..Our results indicate that BrlR acts as an activator of multidrug efflux pumps to confer tolerance to P. aeruginosa biofilms and to resist the action of antimicrobial agents. ..
- Baraquet C, Harwood C. Cyclic diguanosine monophosphate represses bacterial flagella synthesis by interacting with the Walker A motif of the enhancer-binding protein FleQ. Proc Natl Acad Sci U S A. 2013;110:18478-83 pubmed publisher..aeruginosa. The Walker A motif of FleQ is perfectly conserved, opening up the possibility that other AAA+ ATPases may respond to c-di-GMP. ..
- De N, Pirruccello M, Krasteva P, Bae N, Raghavan R, Sondermann H. Phosphorylation-independent regulation of the diguanylate cyclase WspR. PLoS Biol. 2008;6:e67 pubmed publisher..A structural comparison reveals resemblance of the oligomeric states to assemblies of GAF domains, widely used regulatory domains in signaling molecules conserved from archaea to mammals, suggesting a similar mechanism of regulation...
- Goldová J, Ulrych A, Hercík K, Branny P. A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. BMC Genomics. 2011;12:437 pubmed publisher..Based on these data, it is likely that the reduced virulence of the mutant strain results from an impaired ability to survive in the host due to the limited response to stress conditions. ..
- Huang J, Sonnleitner E, Ren B, Xu Y, Haas D. Catabolite repression control of pyocyanin biosynthesis at an intersection of primary and secondary metabolism in Pseudomonas aeruginosa. Appl Environ Microbiol. 2012;78:5016-20 pubmed publisher..Crc bound to an extended imperfect recognition sequence that was interrupted by the AUG translation initiation codon. ..
- Milojevic T, Grishkovskaya I, Sonnleitner E, Djinovic Carugo K, Blasi U. The Pseudomonas aeruginosa catabolite repression control protein Crc is devoid of RNA binding activity. PLoS ONE. 2013;8:e64609 pubmed publisher..Taken together, these data challenge a role of Crc as a direct translational repressor in carbon catabolite repression in P. aeruginosa. ..
- Vance R, Rietsch A, Mekalanos J. Role of the type III secreted exoenzymes S, T, and Y in systemic spread of Pseudomonas aeruginosa PAO1 in vivo. Infect Immun. 2005;73:1706-13 pubmed..Furthermore, our findings suggest that the effector-independent popB-dependent cytotoxicity that we and others have observed in vitro in macrophage cell lines may not be of great importance in vivo. ..
- Miller D, Zhang Y, Rock C, White S. Structure of RhlG, an essential beta-ketoacyl reductase in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa. J Biol Chem. 2006;281:18025-32 pubmed..These structural and biochemical studies establish RhlG as a NADPH-dependent beta-ketoacyl reductase of the SDR protein superfamily and further suggest that the ACP of fatty acid synthesis does not carry the substrates for RhlG. ..
- Brutinel E, Vakulskas C, Brady K, Yahr T. Characterization of ExsA and of ExsA-dependent promoters required for expression of the Pseudomonas aeruginosa type III secretion system. Mol Microbiol. 2008;68:657-71 pubmed publisher..The combined data support a model in which two ExsA(His) molecules bind adjacent sites on the promoter to activate T3SS gene transcription. ..
- Farrow J, Sund Z, Ellison M, Wade D, Coleman J, Pesci E. PqsE functions independently of PqsR-Pseudomonas quinolone signal and enhances the rhl quorum-sensing system. J Bacteriol. 2008;190:7043-51 pubmed publisher..Overall, our data lead us to conclude that PqsE functions as a regulator that is independent of PqsR and PQS but dependent on the rhl quorum-sensing system. ..
- Damron F, Qiu D, Yu H. The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis. J Bacteriol. 2009;191:2285-95 pubmed publisher..Our results also indicate that AlgW-mediated MucA degradation required algB and rpoN in the kinB mutant. Collectively, these studies indicate that KinB is a negative regulator of alginate production in wt mucA strain PAO1. ..
- Rampioni G, Pustelny C, Fletcher M, Wright V, Bruce M, Rumbaugh K, et al. Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts. Environ Microbiol. 2010;12:1659-73 pubmed publisher..Taken together these data reveal that PqsE is a key regulator within the QS circuitry facilitating the environmental adaptation of P. aeruginosa. ..
- Damron F, Davis M, Withers T, Ernst R, Goldberg J, Yu G, et al. Vanadate and triclosan synergistically induce alginate production by Pseudomonas aeruginosa strain PAO1. Mol Microbiol. 2011;81:554-70 pubmed publisher..Altogether, these findings suggest a model of alginate induction and the PIA-AMV medium may be suitable for examining early lung colonization phenotypes in CF before the selection of the mucA mutants. ..
- Murina V, Lekontseva N, Nikulin A. Hfq binds ribonucleotides in three different RNA-binding sites. Acta Crystallogr D Biol Crystallogr. 2013;69:1504-13 pubmed publisher..The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface. ..
- Balyimez A, Colmer Hamood J, San Francisco M, Hamood A. Characterization of the Pseudomonas aeruginosa metalloendopeptidase, Mep72, a member of the Vfr regulon. BMC Microbiol. 2013;13:269 pubmed publisher..coli. Vfr directly regulates the expression of the PA2782-mep72 operon by binding to its upstream region. However, unlike other Vfr-targeted genes, Vfr binding does not require an intact Vfr consensus binding sequence. ..
- Liang H, Li L, Dong Z, Surette M, Duan K. The YebC family protein PA0964 negatively regulates the Pseudomonas aeruginosa quinolone signal system and pyocyanin production. J Bacteriol. 2008;190:6217-27 pubmed publisher..The results added another layer of regulation in the rather complex quorum-sensing systems in P. aeruginosa and demonstrated a clear functional clue for the YebC family proteins. ..
- Dekimpe V, Déziel E. Revisiting the quorum-sensing hierarchy in Pseudomonas aeruginosa: the transcriptional regulator RhlR regulates LasR-specific factors. Microbiology. 2009;155:712-23 pubmed publisher..This work demonstrates that the QS hierarchy is more complex than the model simply presenting the las system above the rhl system. ..
- Beaufort N, Corvazier E, Hervieu A, Choqueux C, Dussiot M, Louedec L, et al. The thermolysin-like metalloproteinase and virulence factor LasB from pathogenic Pseudomonas aeruginosa induces anoikis of human vascular cells. Cell Microbiol. 2011;13:1149-67 pubmed publisher..Thus, among P. aeruginosa-secreted metalloproteinases, LasB can induce vascular cell anoikis through simultaneous proteolysis of ECM components and cell receptors, suggesting the uPAR-vitronectin axis as a major target in this process. ..
- Toutain C, Zegans M, O Toole G. Evidence for two flagellar stators and their role in the motility of Pseudomonas aeruginosa. J Bacteriol. 2005;187:771-7 pubmed..Either stator is sufficient for swimming, but both are necessary for swarming motility under most of the conditions tested, suggesting that these two stators may have different roles in these two types of motility. ..
- Chieda Y, Iiyama K, Yasunaga Aoki C, Lee J, Kusakabe T, Shimizu S. Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori. FEMS Microbiol Lett. 2005;244:181-6 pubmed publisher..However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae...
- Sonnleitner E, Schuster M, Sorger Domenigg T, Greenberg E, Blasi U. Hfq-dependent alterations of the transcriptome profile and effects on quorum sensing in Pseudomonas aeruginosa. Mol Microbiol. 2006;59:1542-58 pubmed..The model is corroborated by the observation that a rsmY(-) mutant mimics the hfq(-) phenotype with regard to rhlI expression. ..
- Agarwal R, Bonanno J, Burley S, Swaminathan S. Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal. Acta Crystallogr D Biol Crystallogr. 2006;62:383-91 pubmed publisher..28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported...
- Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, et al. Structural basis of biological N2O generation by bacterial nitric oxide reductase. Science. 2010;330:1666-70 pubmed publisher..Protons required for the NOR reaction are probably provided from the extracellular side. ..
- Croda García G, Grosso Becerra V, González Valdez A, Servín Gonzalez L, Soberón Chávez G. Transcriptional regulation of Pseudomonas aeruginosa rhlR: role of the CRP orthologue Vfr (virulence factor regulator) and quorum-sensing regulators LasR and RhlR. Microbiology. 2011;157:2545-55 pubmed publisher..We also show that rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter. This positive autoregulation plays a major role in rhlR expression. ..
- Sun Y, Karmakar M, Taylor P, Rietsch A, Pearlman E. ExoS and ExoT ADP ribosyltransferase activities mediate Pseudomonas aeruginosa keratitis by promoting neutrophil apoptosis and bacterial survival. J Immunol. 2012;188:1884-95 pubmed publisher..aeruginosa keratitis in ExoS- and ExoT-producing strains is almost entirely due to their ADPR activities, which subvert the host response by targeting the antibacterial activity of infiltrating neutrophils...
- Liang H, Deng X, Ji Q, Sun F, Shen T, He C. The Pseudomonas aeruginosa global regulator VqsR directly inhibits QscR to control quorum-sensing and virulence gene expression. J Bacteriol. 2012;194:3098-108 pubmed publisher..Taken together, these findings provide new insights into the complex regulation network of QS systems in P. aeruginosa. ..
- Baraquet C, Murakami K, Parsek M, Harwood C. The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP. Nucleic Acids Res. 2012;40:7207-18 pubmed publisher..The mechanism of action of FleQ is distinct from that of other bacterial transcription factors that both activate and repress gene expression from a single promoter. ..
- Westwood I, Holton S, Rodrigues Lima F, Dupret J, Bhakta S, Noble M, et al. Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase. Biochem J. 2005;385:605-12 pubmed..The recombinant protein has been expressed in sufficient quantity to allow protein crystallization, and we have subsequently determined the 1.95 A structure of PANAT by X-ray crystallography. ..
- Oinuma K, Greenberg E. Acyl-homoserine lactone binding to and stability of the orphan Pseudomonas aeruginosa quorum-sensing signal receptor QscR. J Bacteriol. 2011;193:421-8 pubmed publisher..Our data support a model whereby QscR polypeptides fold properly in the absence of an acyl-HSL, but soluble, acyl-HSL-free QscR does not accumulate because it is subject to rapid aggregation or proteolysis. ..
- O Connor J, Kuwada N, Huangyutitham V, Wiggins P, Harwood C. Surface sensing and lateral subcellular localization of WspA, the receptor in a chemosensory-like system leading to c-di-GMP production. Mol Microbiol. 2012;86:720-9 pubmed publisher..Thus, the amino acid sequence of the WspA periplasmic region did not need to be conserved for the Wsp system to respond to surfaces. ..
- Colmer Hamood J, Aramaki H, Gaines J, Hamood A. Transcriptional analysis of the Pseudomonas aeruginosa toxA regulatory gene ptxR. Can J Microbiol. 2006;52:343-56 pubmed..RNA polymerase reconstituted with either RpoS or AlgU produced no transcripts. However, a transcript was produced by RpoH-reconstituted RNA polymerase. ..
- Zaborina O, Lepine F, Xiao G, Valuckaite V, Chen Y, Li T, et al. Dynorphin activates quorum sensing quinolone signaling in Pseudomonas aeruginosa. PLoS Pathog. 2007;3:e35 pubmed..Taken together, these data demonstrate that P. aeruginosa can intercept opioid compounds released during host stress and integrate them into core elements of quorum sensing circuitry leading to enhanced virulence. ..
- Abeyrathne P, Lam J. WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core. Mol Microbiol. 2007;65:1345-59 pubmed..A waaL mutant of P. aeruginosa could not be cross-complemented by waaL of Escherichia coli, which suggested that each of these proteins has specificity for its cognate core oligosaccharide. ..
- Zamorano L, Reeve T, Deng L, Juan C, Moya B, Cabot G, et al. NagZ inactivation prevents and reverts beta-lactam resistance, driven by AmpD and PBP 4 mutations, in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2010;54:3557-63 pubmed publisher..Therefore, NagZ is envisaged to be a candidate target for preventing and reverting beta-lactam resistance in P. aeruginosa. ..
- Liang H, Duan J, Sibley C, Surette M, Duan K. Identification of mutants with altered phenazine production in Pseudomonas aeruginosa. J Med Microbiol. 2011;60:22-34 pubmed publisher..These findings suggest that qteE plays an important role in P. aeruginosa pathogenicity and is part of the regulatory networks controlling phenazine production. ..
- Angus A, Evans D, Barbieri J, Fleiszig S. The ADP-ribosylation domain of Pseudomonas aeruginosa ExoS is required for membrane bleb niche formation and bacterial survival within epithelial cells. Infect Immun. 2010;78:4500-10 pubmed publisher..aeruginosa bleb niches and intracellular survival involve ExoS ADP-r activity and implicate a connection between bleb niche formation and the known role(s) of ExoS-mediated apoptosis and/or Rab GTPase inactivation. ..
- Roy A, Petrova O, Sauer K. The phosphodiesterase DipA (PA5017) is essential for Pseudomonas aeruginosa biofilm dispersion. J Bacteriol. 2012;194:2904-15 pubmed publisher..Dispersion was found to require protein synthesis and resulted in increased dipA expression and reduction of c-di-GMP levels. We propose a role of DipA in enabling dispersion in P. aeruginosa biofilms. ..
- Abeyrathne P, Daniels C, Poon K, Matewish M, Lam J. Functional characterization of WaaL, a ligase associated with linking O-antigen polysaccharide to the core of Pseudomonas aeruginosa lipopolysaccharide. J Bacteriol. 2005;187:3002-12 pubmed..These results demonstrate that waaL in P. aeruginosa encodes a functional O-antigen ligase that is important for cell wall integrity and motility of the bacteria. ..
- Siehnel R, Traxler B, An D, Parsek M, Schaefer A, Singh P. A unique regulator controls the activation threshold of quorum-regulated genes in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2010;107:7916-21 pubmed publisher..This unique regulator governs two central QS control points in P. aeruginosa and shapes the expression pattern thought fundamental to the biological functions of QS. ..
- Chen H, Yi C, Zhang J, Zhang W, Ge Z, Yang C, et al. Structural insight into the oxidation-sensing mechanism of the antibiotic resistance of regulator MexR. EMBO Rep. 2010;11:685-90 pubmed publisher..1 A. This crystal structure reveals the mechanism by which oxidative signal allosterically derepresses the MexR-controlled transcription activation. ..
- Moscoso J, Mikkelsen H, Heeb S, Williams P, Filloux A. The Pseudomonas aeruginosa sensor RetS switches type III and type VI secretion via c-di-GMP signalling. Environ Microbiol. 2011;13:3128-38 pubmed publisher..These results provide a firm link between the RetS/GacS and the c-di-GMP pathways, which coordinate bacterial lifestyles, as well as secretion systems that determine the infection strategy of P. aeruginosa. ..
- Daddaoua A, Fillet S, Fernandez M, Udaondo Z, Krell T, Ramos J. Genes for carbon metabolism and the ToxA virulence factor in Pseudomonas aeruginosa are regulated through molecular interactions of PtxR and PtxS. PLoS ONE. 2012;7:e39390 pubmed publisher..Data suggest two different mechanisms of control exerted by the same regulator. ..
- Russell A, LeRoux M, Hathazi K, Agnello D, Ishikawa T, Wiggins P, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013;496:508-12 pubmed publisher..The surprising finding that virulence-associated phospholipases can serve as specific antibacterial effectors suggests that interbacterial interactions are a relevant factor driving the continuing evolution of pathogenesis. ..
- Romsang A, Atichartpongkul S, Trinachartvanit W, Vattanaviboon P, Mongkolsuk S. Gene expression and physiological role of Pseudomonas aeruginosa methionine sulfoxide reductases during oxidative stress. J Bacteriol. 2013;195:3299-308 pubmed publisherb>Pseudomonas aeruginosa PAO1 has two differentially expressed methionine sulfoxide reductase genes: msrA (PA5018) and msrB (PA2827)...
- Balasubramanian D, Kumari H, Jaric M, Fernandez M, Turner K, Dove S, et al. Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response. Nucleic Acids Res. 2014;42:979-98 pubmed publisher..This study further attests to the role of AmpR in regulating virulence and physiological processes in P. aeruginosa. ..
- Ruer S, Ball G, Filloux A, de Bentzmann S. The 'P-usher', a novel protein transporter involved in fimbrial assembly and TpsA secretion. EMBO J. 2008;27:2669-80 pubmed publisher..The P-usher defines a novel transport pathway involving a molecular machine made with old spare parts...
- Bazire A, Shioya K, Soum Soutera E, Bouffartigues E, Ryder C, Guentas Dombrowsky L, et al. The sigma factor AlgU plays a key role in formation of robust biofilms by nonmucoid Pseudomonas aeruginosa. J Bacteriol. 2010;192:3001-10 pubmed publisher..Expressing the ppyR gene in PAOU partially restored the formation of robust biofilms. ..
- Kuang Z, Hao Y, Hwang S, Zhang S, Kim E, Akinbi H, et al. The Pseudomonas aeruginosa flagellum confers resistance to pulmonary surfactant protein-A by impacting the production of exoproteases through quorum-sensing. Mol Microbiol. 2011;79:1220-35 pubmed publisher..Thus, reduced ability of ?fliC bacteria to quorum-sense attenuates production of exoproteases and limits degradation of SP-A, thereby conferring susceptibility to this major pulmonary host defence protein. ..
- Jones C, Ryder C, Mann E, Wozniak D. AmrZ modulates Pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon. J Bacteriol. 2013;195:1637-44 pubmed publisher..aeruginosa biofilm infections from colonizing to chronic biofilms through repression of the psl operon while activating the algD operon...
- Pastor A, Chabert J, Louwagie M, Garin J, Attree I. PscF is a major component of the Pseudomonas aeruginosa type III secretion needle. FEMS Microbiol Lett. 2005;253:95-101 pubmed..YscF of Yersinia enterocolitica was able to functionally complement the pscF deletion, but required 11 P. aeruginosa-specific amino acids at the N-terminus for its function. ..
- Liang H, Li L, Kong W, Shen L, Duan K. Identification of a novel regulator of the quorum-sensing systems in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2009;293:196-204 pubmed publisher..This adds an important new player in the complex quorum-sensing network in P. aeruginosa. ..
- Sayner S, Frank D, King J, Chen H, VandeWaa J, Stevens T. Paradoxical cAMP-induced lung endothelial hyperpermeability revealed by Pseudomonas aeruginosa ExoY. Circ Res. 2004;95:196-203 pubmed..These findings collectively illustrate a previously unrecognized mechanism of hyperpermeability induced by rises in cytosolic cAMP. ..
- Leduc D, Beaufort N, de Bentzmann S, Rousselle J, Namane A, Chignard M, et al. The Pseudomonas aeruginosa LasB metalloproteinase regulates the human urokinase-type plasminogen activator receptor through domain-specific endoproteolysis. Infect Immun. 2007;75:3848-58 pubmed..The LasB proteinase is thus endowed with a high potential for the alteration of uPAR expression and functioning on inflammatory cells during infections by P. aeruginosa. ..
- Cisz M, Lee P, Rietsch A. ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa. J Bacteriol. 2008;190:2726-38 pubmed..The requirement for a host cell cofactor to control effector secretion may help explain the recently observed phenomenon of target cell specificity in both the Yersinia and P. aeruginosa type III secretion systems. ..
- Qiu D, Eisinger V, Head N, Pier G, Yu H. ClpXP proteases positively regulate alginate overexpression and mucoid conversion in Pseudomonas aeruginosa. Microbiology. 2008;154:2119-30 pubmed publisher..The ClpXP and ClpP2 proteins appear to be part of a proteolytic network that degrades the cytoplasmic portion of truncated MucA proteins to release the sequestered AlgU, which drives alginate biosynthesis. ..
- Chemani C, Imberty A, de Bentzmann S, Pierre M, Wimmerova M, Guery B, et al. Role of LecA and LecB lectins in Pseudomonas aeruginosa-induced lung injury and effect of carbohydrate ligands. Infect Immun. 2009;77:2065-75 pubmed publisher..Our results demonstrate that there is a relationship between lectins and the pathogenicity of P. aeruginosa. Inhibition of the lectins by specific carbohydrates may provide new therapeutic perspectives. ..
- Cezairliyan B, Sauer R. Control of Pseudomonas aeruginosa AlgW protease cleavage of MucA by peptide signals and MucB. Mol Microbiol. 2009;72:368-79 pubmed publisher..We demonstrate that tight binding of MucB to MucA strongly inhibits this cleavage. We also probe the roles of structural features of AlgW, including a key regulatory loop and its PDZ domain, in regulating substrate binding and cleavage. ..
- Morita Y, Gilmour C, Metcalf D, Poole K. Translational control of the antibiotic inducibility of the PA5471 gene required for mexXY multidrug efflux gene expression in Pseudomonas aeruginosa. J Bacteriol. 2009;191:4966-75 pubmed publisher..aeruginosa. These data suggest that PA5471 is not sufficient for MexXY recruitment in response to antibiotic exposure and that additional antibiotic-dependent effects are needed. ..
- Brencic A, McFarland K, McManus H, Castang S, Mogno I, Dove S, et al. The GacS/GacA signal transduction system of Pseudomonas aeruginosa acts exclusively through its control over the transcription of the RsmY and RsmZ regulatory small RNAs. Mol Microbiol. 2009;73:434-45 pubmed publisher..This work highlights the importance of post-transcriptional mechanisms involving sRNAs in controlling gene expression during bacterial adaptation to different environments. ..
- Byrd M, Sadovskaya I, Vinogradov E, Lu H, Sprinkle A, Richardson S, et al. Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production. Mol Microbiol. 2009;73:622-38 pubmed publisher..The structural and genetic data presented here provide a basis for further investigation of the Psl proteins and potential roles for Psl in the biology and pathogenesis of P. aeruginosa. ..
- Schuster M, Urbanowski M, Greenberg E. Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Proc Natl Acad Sci U S A. 2004;101:15833-9 pubmed..aeruginosa quorum sensing. Insights into the molecular mechanism of LasR function have implications for the development of quorum-sensing targeted antivirulence compounds. ..
- Linares J, Lopez J, Camafeita E, Albar J, Rojo F, Martinez J. Overexpression of the multidrug efflux pumps MexCD-OprJ and MexEF-OprN is associated with a reduction of type III secretion in Pseudomonas aeruginosa. J Bacteriol. 2005;187:1384-91 pubmed..The relevance of potential metabolic and quorum-sensing imbalances due to overexpression of MDR pumps associated with this phenotype is also discussed. ..
- Carty N, Layland N, Colmer Hamood J, Calfee M, Pesci E, Hamood A. PtxR modulates the expression of QS-controlled virulence factors in the Pseudomonas aeruginosa strain PAO1. Mol Microbiol. 2006;61:782-94 pubmed..These results suggest that PtxR negatively regulates the expression of the rhamnolipid and pyocyanin genes through rhlI and the pqsABCDE operon while it positively regulates the expression of lasB through lasI. ..
- Güvener Z, Harwood C. Subcellular location characteristics of the Pseudomonas aeruginosa GGDEF protein, WspR, indicate that it produces cyclic-di-GMP in response to growth on surfaces. Mol Microbiol. 2007;66:1459-73 pubmed..Our results suggest that the compartmentalized production of c-di-GMP in response to a stimulus associated with growth on a surface is an important functional characteristic of the Wsp system. ..
- Marshall B, Stintzi A, Gilmour C, Meyer J, Poole K. Citrate-mediated iron uptake in Pseudomonas aeruginosa: involvement of the citrate-inducible FecA receptor and the FeoB ferrous iron transporter. Microbiology. 2009;155:305-15 pubmed publisher..These data indicate that Fe delivered to P. aeruginosa by citrate is released as Fe2+, probably in the periplasm, prior to its transport into cells via Fe transport components. ..
- Morita Y, Cao L, Gould V, Avison M, Poole K. nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. J Bacteriol. 2006;188:8649-54 pubmed..Moreover, increased expression from this promoter was seen in a nalD mutant, consistent with NalD directly controlling mexAB-oprM expression from a second promoter. ..
- Urbanowski M, Brutinel E, Yahr T. Translocation of ExsE into Chinese hamster ovary cells is required for transcriptional induction of the Pseudomonas aeruginosa type III secretion system. Infect Immun. 2007;75:4432-9 pubmed
- Merighi M, Lee V, Hyodo M, Hayakawa Y, Lory S. The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Mol Microbiol. 2007;65:876-95 pubmed..Alg44 appears to be an essential component of the alginate biosynthetic apparatus, where, following binding of c-di-GMP, it controls polymerization or transport of the polysaccharide. ..
- Rampioni G, Schuster M, Greenberg E, Bertani I, Grasso M, Venturi V, et al. RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa. Mol Microbiol. 2007;66:1557-65 pubmed..These investigations highlight the importance of RsaL as a global regulator of P. aeruginosa physiology that provides a counterbalance to 3OC(12)-HSL-dependent gene activation via multiple mechanisms. ..
- Hickman J, Harwood C. Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor. Mol Microbiol. 2008;69:376-89 pubmed publisher..Our results show that FleQ is a new type of c-di-GMP binding protein that controls the transcriptional regulation of EPS biosynthesis genes in P. aeruginosa. ..
- Zhang Y, Frank M, Zhu K, Mayasundari A, Rock C. PqsD is responsible for the synthesis of 2,4-dihydroxyquinoline, an extracellular metabolite produced by Pseudomonas aeruginosa. J Biol Chem. 2008;283:28788-94 pubmed publisher..DHQ was produced by Escherichia coli coexpressing PqsA and PqsD, illustrating that these two proteins are the only factors necessary for DHQ synthesis. Thus, PqsD is responsible for the production of DHQ in P. aeruginosa. ..