Haemophilus influenzae Rd KW20

Summary

Alias: Haemophilus influenzae KW20, Haemophilus influenzae Rd

Top Publications

  1. Kidd S, Jiang D, Jennings M, McEwan A. Glutathione-dependent alcohol dehydrogenase AdhC is required for defense against nitrosative stress in Haemophilus influenzae. Infect Immun. 2007;75:4506-13 pubmed
    In Haemophilus influenzae Rd KW20, we identified a gene, adhC, which encodes a class III alcohol dehydrogenase (AdhC) and has S-nitrosoglutathione reductase activity. adhC exists on an operon with estD, which encodes an esterase...
  2. Jiang D, Tikhomirova A, Bent S, Kidd S. A discrete role for FNR in the transcriptional response to moderate changes in oxygen by Haemophilus influenzae Rd KW20. Res Microbiol. 2016;167:103-13 pubmed publisher
    ..It was the changed whole cell profile that impacted on ROS/RNS defence, but surprisingly, the FNR-regulated, anaerobic nitrite reductase (NrfA) continued to be expressed and had a role in this phenotype. ..
  3. Walter R, Stuy J. Isolation and characterization of a UV-sensitive mutator (mutB1) mutant of Haemophilus influenzae. J Bacteriol. 1988;170:2537-42 pubmed
    ..This mutant did not degrade its DNA following exposure to UV. It is speculated that the mutB1 mutation is similar to the Escherichia coli uvrD mutation. ..
  4. Zhu Q, Zhao S, Somerville R. Expression, purification, and functional analysis of the TyrR protein of Haemophilus influenzae. Protein Expr Purif. 1997;10:237-46 pubmed
    The gene that was inferred to encode the TyrR protein of Haemophilus influenzae Rd was synthesized by polymerase chain reaction and inserted into a T7-based expression vector. Methods were developed to overexpress the TyrR protein of H...
  5. Lewis H, Furlong E, Laubert B, Eroshkina G, Batiyenko Y, Adams J, et al. A structural genomics approach to the study of quorum sensing: crystal structures of three LuxS orthologs. Structure. 2001;9:527-37 pubmed
    ..Specifically, acting as a homodimer, the protein binds a methionine analog, S-ribosylhomocysteine (SRH). The zinc atom is in position to cleave the ribose ring in a step along the synthesis pathway of AI-2...
  6. Weiser J, Love J, Moxon E. The molecular mechanism of phase variation of H. influenzae lipopolysaccharide. Cell. 1989;59:657-65 pubmed
    ..Phase variation also allows for selection of organisms displaying certain LPS epitopes in vivo...
  7. Loosmore S, Yang Y, Coleman D, Shortreed J, England D, Klein M. Outer membrane protein D15 is conserved among Haemophilus influenzae species and may represent a universal protective antigen against invasive disease. Infect Immun. 1997;65:3701-7 pubmed
    ..Thus, D15 is a highly conserved antigen that is protective in animal models and it may be a useful component of a universal subunit vaccine against Haemophilus infection and disease...
  8. Cirilli M, Zheng R, Scapin G, Blanchard J. Structural symmetry: the three-dimensional structure of Haemophilus influenzae diaminopimelate epimerase. Biochemistry. 1998;37:16452-8 pubmed publisher
  9. Zhang M, Monzingo A, Segatori L, Georgiou G, Robertus J. Structure of DsbC from Haemophilus influenzae. Acta Crystallogr D Biol Crystallogr. 2004;60:1512-8 pubmed
    ..influenzae protein allows the movement of the N-terminal domain with respect to the C-terminal domain through motions in the flexible hinge, generating high thermal parameters and unusually high anisotropy in the crystallographic data. ..

More Information

Publications163 found, 100 shown here

  1. Mirza I, Nazi I, Korczynska M, Wright G, Berghuis A. Crystal structure of homoserine transacetylase from Haemophilus influenzae reveals a new family of alpha/beta-hydrolases. Biochemistry. 2005;44:15768-73 pubmed publisher
    ..Furthermore, the properties of the tunnel provide a rationale for how homoserine transacetylase catalyzes a transferase reaction vs hydrolysis, despite extensive similarity in active site architecture to hydrolytic enzymes...
  2. Maskell D. Cloning and sequencing of the Haemophilus influenzae aroA gene. Gene. 1993;129:155-6 pubmed
    ..The stop codon of the putative H. influenzae purN gene overlaps the start codon for H. influenzae aroA, suggesting that the two genes may form an operon and that they may be translationally coupled. ..
  3. Lange B, Rujan T, Martin W, Croteau R. Isoprenoid biosynthesis: the evolution of two ancient and distinct pathways across genomes. Proc Natl Acad Sci U S A. 2000;97:13172-7 pubmed publisher
    ..The results suggest that lateral gene transfer between eubacteria subsequent to the origin of plastids has played a major role in the evolution of this pathway...
  4. Morton D, Smith A, VanWagoner T, Seale T, Whitby P, Stull T. Lipoprotein e (P4) of Haemophilus influenzae: role in heme utilization and pathogenesis. Microbes Infect. 2007;9:932-9 pubmed publisher
    ..In addition, a rat model of infection demonstrated a role for e (P4) in the pathogenesis of invasive disease...
  5. Sharma U, Dwarakanath P, Banerjee N, Town C, Balganesh T. Expression and characterization of the ponA (ORF I) gene of Haemophilus influenzae: functional complementation in a heterologous system. J Bacteriol. 1995;177:6745-50 pubmed
    ..coli delta ponA ponB::spcr expressing the ORF I gene, and the amino acid alignment of the PBP 1 family of high-molecular-weight PBPs group the ORF I protein into the PBP 1A family of high-molecular-weight PBPs. ..
  6. Sousa M, McKay D. Structure of the universal stress protein of Haemophilus influenzae. Structure. 2001;9:1135-41 pubmed
    ..By implication, one subset of the universal stress proteins presumably has an ATP-dependent function, while another subset functions in ATP-independent activities...
  7. Kwon A, Trame C, McKay D. Kinetics of protein substrate degradation by HslUV. J Struct Biol. 2004;146:141-7 pubmed
    ..The fall-off in degradation by HslUV for the more stable substrates that are degraded by ClpXP is consistent with the two systems acting on different spectra of biological substrates. ..
  8. Larson T, Goodgal S. Sequence and transcriptional regulation of com101A, a locus required for genetic transformation in Haemophilus influenzae. J Bacteriol. 1991;173:4683-91 pubmed
    A 2.8-kb EcoRI-BglII fragment cloned from the wild-type Haemophilus influenzae Rd chromosome is shown to increase the transformability of the Com-101 mutant through trans complementation...
  9. Singh B, Al Jubair T, Förnvik K, Thunnissen M, Riesbeck K. Crystallization and X-ray diffraction analysis of a novel surface-adhesin protein: protein E from Haemophilus influenzae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:222-6 pubmed publisher
    ..Diffraction data collected from native and SeMet-derivative crystals extended to resolutions of 1.8 and 2.6 Å, respectively. ..
  10. Loosmore S, Yang Y, Coleman D, Shortreed J, England D, Harkness R, et al. Cloning and expression of the Haemophilus influenzae transferrin receptor genes. Mol Microbiol. 1996;19:575-86 pubmed
    ..influenzae strains, as determined by Western blot analysis. In a passive model of bacteraemia, infant rats were protected from challenge with Hib after transfer of anti-rTbp2 antiserum, but not after anti-rTbp1 antiserum. ..
  11. Mulligan C, Geertsma E, Severi E, Kelly D, Poolman B, Thomas G. The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter. Proc Natl Acad Sci U S A. 2009;106:1778-83 pubmed publisher
    ..Importantly, the binding protein also confers directionality on the transporter, and reversal of sialic acid transport from import to export is only possible in the presence of an excess of unliganded SiaP...
  12. Mol C, Brooun A, Dougan D, Hilgers M, Tari L, Wijnands R, et al. Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae. J Bacteriol. 2003;185:4152-62 pubmed
  13. Lehmann C, Lim K, Chalamasetty V, Krajewski W, Melamud E, Galkin A, et al. The HI0073/HI0074 protein pair from Haemophilus influenzae is a member of a new nucleotidyltransferase family: structure, sequence analyses, and solution studies. Proteins. 2003;50:249-60 pubmed publisher
    ..Double- and single-stranded DNA binding assays showed no evidence of DNA binding to HI0074 or to HI0073/HI0074 complex despite the suggestive shape of the putative binding cleft formed by the HI0074 dimer...
  14. Santos Lopez A, Bernabe Balas C, Ares Arroyo M, Ortega Huedo R, Hoefer A, San Millan A, et al. A Naturally Occurring Single Nucleotide Polymorphism in a Multicopy Plasmid Produces a Reversible Increase in Antibiotic Resistance. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..Using the Haemophilus influenzae Rd KW20 strain as a model system, we combined antibiotic susceptibility tests, quantitative PCRs, competition ..
  15. Skaare D, Anthonisen I, Kahlmeter G, Matuschek E, Natås O, Steinbakk M, et al. Emergence of clonally related multidrug resistant Haemophilus influenzae with penicillin-binding protein 3-mediated resistance to extended-spectrum cephalosporins, Norway, 2006 to 2013. Euro Surveill. 2014;19: pubmed
    ..Prior to this study, no multidrug resistant high-rPBP3 H. influenzae had been reported in Norway. Intensified surveillance of antimicrobial resistance is needed to guide empiric therapy. ..
  16. Jiang D, Tikhomirova A, Kidd S. Haemophilus influenzae strains possess variations in the global transcriptional profile in response to oxygen levels and this influences sensitivity to environmental stresses. Res Microbiol. 2016;167:13-9 pubmed publisher
    An alcohol dehydrogenase, AdhC, is required for Haemophilus influenzae Rd KW20 growth with high oxygen. AdhC protects against both exogenous and metabolically generated, endogenous reactive aldehydes...
  17. Willis M, Zhuang Z, Song F, Howard A, Dunaway Mariano D, Herzberg O. Structure of YciA from Haemophilus influenzae (HI0827), a hexameric broad specificity acyl-coenzyme A thioesterase. Biochemistry. 2008;47:2797-805 pubmed publisher
    The crystal structure of HI0827 from Haemophilus influenzae Rd KW20, initially annotated "hypothetical protein" in sequence databases, exhibits an acyl-coenzyme A (acyl-CoA) thioesterase "hot dog" fold with a trimer of dimers oligomeric ..
  18. Parsons L, Grishaev A, Bax A. The periplasmic domain of TolR from Haemophilus influenzae forms a dimer with a large hydrophobic groove: NMR solution structure and comparison to SAXS data. Biochemistry. 2008;47:3131-42 pubmed publisher
  19. O Neill J, St Geme J, Cutter D, Adderson E, Anyanwu J, Jacobs R, et al. Invasive disease due to nontypeable Haemophilus influenzae among children in Arkansas. J Clin Microbiol. 2003;41:3064-9 pubmed
    ..influenzae may have more invasive potential, especially in young children and patients with underlying medical conditions. At this point, the specific factors that contribute to enhanced virulence remain unclear...
  20. Chandler M. The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd. Proc Natl Acad Sci U S A. 1992;89:1626-30 pubmed
    The Haemophilus influenzae Rd strain JG87 contains a single mini-Tn10kan insertion that causes a deficiency in the development of competence for genetic transformation...
  21. Ito H, Sadaoka A, Kotani H, Hiraoka N, Nakamura T. Cloning, nucleotide sequence, and expression of the HincII restriction-modification system. Nucleic Acids Res. 1990;18:3903-11 pubmed
    ..influenzae Rc. The amino acid sequence of M.HincII was compared with the sequences of four other adenine-specific type II methylases. Important homology was found between tne M.HincII and these other methylases...
  22. Munson R, Grass S, West R. Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae. Infect Immun. 1993;61:4017-20 pubmed
    ..The gene for outer membrane protein P5 was sequenced. The mature protein has a molecular weight of 35,628. The protein is 50% identical and 65% similar to the OmpA protein of Escherichia coli. ..
  23. Mochalkin I, Lightle S, Zhu Y, Ohren J, Spessard C, Chirgadze N, et al. Characterization of substrate binding and catalysis in the potential antibacterial target N-acetylglucosamine-1-phosphate uridyltransferase (GlmU). Protein Sci. 2007;16:2657-66 pubmed
  24. Baelen S, DeWitte F, Clantin B, Villeret V. Structure of the secretion domain of HxuA from Haemophilus influenzae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69:1322-7 pubmed publisher
  25. Jarosik G, Sanders J, Cope L, Muller Eberhard U, Hansen E. A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b. Infect Immun. 1994;62:2470-7 pubmed
    ..These results indicate that the H. influenzae TonB protein is required not only for heme utilization by this pathogen in vitro, but also for virulence of H. influenzae type b in an animal model...
  26. Brooke J, Valvano M. Molecular cloning of the Haemophilus influenzae gmhA (lpcA) gene encoding a phosphoheptose isomerase required for lipooligosaccharide biosynthesis. J Bacteriol. 1996;178:3339-41 pubmed
    ..coli strain chi711. Amino acid sequence comparisons of the GmhA proteins of E. coli and H. influenzae with other proteins in the databases revealed the existence of a novel family of phosphosugar a1do-keto isomerases. ..
  27. Servos S, Khan S, Maskell D. Cloning and expression of genes encoding lipid A biosynthesis from Haemophilus influenzae type b. Gene. 1996;175:137-41 pubmed
    ..influenzae lpxA and lpxB are able to complement temperature-sensitive mutants in the equivalent genes in E. coli. This provides evidence that the genetic manipulation of lpx genes to generate altered lipid A molecules may be possible. ..
  28. Brigé A, Cole J, Hagen W, Guisez Y, Van Beeumen J. Overproduction, purification and novel redox properties of the dihaem cytochrome c, NapB, from Haemophilus influenzae. Biochem J. 2001;356:851-8 pubmed
    ..The midpoint reduction potentials of the two haem groups were determined to be -25 mV and -175 mV. These results support our hypothesis that the Nap system fulfils a nitrate-scavenging role in H. influenzae. ..
  29. Broussard T, Pakhomova S, Neau D, Bonnot R, Waldrop G. Structural Analysis of Substrate, Reaction Intermediate, and Product Binding in Haemophilus influenzae Biotin Carboxylase. Biochemistry. 2015;54:3860-70 pubmed publisher
  30. Zulty J, Barcak G. Identification of a DNA transformation gene required for com101A+ expression and supertransformer phenotype in Haemophilus influenzae. Proc Natl Acad Sci U S A. 1995;92:3616-20 pubmed
    ..Collectively, our results suggest that TfoX may play a key role in the development of genetic competence by regulating the expression of late competence-specific genes...
  31. Born T, Franklin M, Blanchard J. Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Haemophilus influenzae met2-encoded homoserine transacetylase. Biochemistry. 2000;39:8556-64 pubmed
    ..On the basis of these observations, we propose a chemical mechanism for this important member of the acyltransferase family and contrast its mechanism with that of homoserine transsuccinylase. ..
  32. Brigé A, Leys D, Van Beeumen J. Crystallization and preliminary X-ray analysis of the recombinant dihaem cytochrome c (NapB) from Haemophilus influenzae. Acta Crystallogr D Biol Crystallogr. 2001;57:418-20 pubmed
    ..Taking the molecular mass of the crystallized form ( approximately 8500 Da) into account, the solvent content was estimated to be 53%, with a V(M) value of 2.64 A(3) Da(-1). ..
  33. Enne V, King A, Livermore D, Hall L. Sulfonamide resistance in Haemophilus influenzae mediated by acquisition of sul2 or a short insertion in chromosomal folP. Antimicrob Agents Chemother. 2002;46:1934-9 pubmed
    ..The two mechanisms of resistance, mediated by sul2 and modified folP, were detected in isolates from both the United Kingdom and Kenya...
  34. Willis M, Song F, Zhuang Z, Krajewski W, Chalamasetty V, Reddy P, et al. Structure of YciI from Haemophilus influenzae (HI0828) reveals a ferredoxin-like alpha/beta-fold with a histidine/aspartate centered catalytic site. Proteins. 2005;59:648-52 pubmed publisher
  35. Mallam A, Jackson S. Probing nature's knots: the folding pathway of a knotted homodimeric protein. J Mol Biol. 2006;359:1420-36 pubmed publisher
    ..Kinetic simulations suggest that all intermediates are on-pathway. These results provide the valuable groundwork required to further understand how Nature codes for knot formation...
  36. Valvano M, Messner P, Kosma P. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology. 2002;148:1979-89 pubmed publisher
  37. Peric M, Bozdogan B, Jacobs M, Appelbaum P. Effects of an efflux mechanism and ribosomal mutations on macrolide susceptibility of Haemophilus influenzae clinical isolates. Antimicrob Agents Chemother. 2003;47:1017-22 pubmed
    ..3%) due to one or several ribosomal mutations. Occasional hypersusceptible strains (1.8%) were found and had no macrolide resistance mechanisms and appeared to be the only truly macrolide-susceptible variants of H. influenzae...
  38. Christensen H, Kuhnert P, Olsen J, Bisgaard M. Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae. Int J Syst Evol Microbiol. 2004;54:1601-9 pubmed publisher
    ..The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves...
  39. Bolon D, Grant R, Baker T, Sauer R. Specificity versus stability in computational protein design. Proc Natl Acad Sci U S A. 2005;102:12724-9 pubmed publisher
  40. Maskell D, Szabo M, Butler P, Williams A, Moxon E. Molecular analysis of a complex locus from Haemophilus influenzae involved in phase-variable lipopolysaccharide biosynthesis. Mol Microbiol. 1991;5:1013-22 pubmed
    ..These mutants had a galE phenotype, as evidenced by galactose sensitivity, altered LPS when grown in the absence of exogenous galactose, and reduced virulence in infant rats...
  41. Ubukata K, Shibasaki Y, Yamamoto K, Chiba N, Hasegawa K, Takeuchi Y, et al. Association of amino acid substitutions in penicillin-binding protein 3 with beta-lactam resistance in beta-lactamase-negative ampicillin-resistant Haemophilus influenzae. Antimicrob Agents Chemother. 2001;45:1693-9 pubmed publisher
    ..Even though mutations in another gene(s) may be involved in beta-lactam resistance, these data indicate that mutations in the ftsI gene are the most important for development of resistance to beta-lactams in BLNAR strains...
  42. Gorman J, Shapiro L. Structure of serine acetyltransferase from Haemophilus influenzae Rd. Acta Crystallogr D Biol Crystallogr. 2004;60:1600-5 pubmed publisher
    The crystal structure of serine acetyltransferase (SAT) from Haemophilus influenzae Rd determined at 2.7 A resolution is presented...
  43. Janson H, Hedén L, Grubb A, Ruan M, Forsgren A. Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli. Infect Immun. 1991;59:119-25 pubmed
    ..The sequence of protein D shows no similarity to those of other immunoglobulin-binding proteins. Protein D is the first example of immunoglobulin receptors from gram-negative bacteria that has been cloned and sequenced. ..
  44. Nwankwo D, Moran L, Slatko B, Waite Rees P, Dorner L, Benner J, et al. Cloning, analysis and expression of the HindIII R-M-encoding genes. Gene. 1994;150:75-80 pubmed
    ..HindIII ENase) and methyltransferase (M.HindIII MTase) from Haemophilus influenzae Rd were cloned and expressed in Escherichia coli and their nucleotide (nt) sequences were determined...
  45. Lim K, Zhang H, Tempczyk A, Bonander N, Toedt J, Howard A, et al. Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine. Proteins. 2001;45:397-407 pubmed publisher
    ..Examination of the Enzyme Commission list of methyltransferases prompted a manual inspection of 10 possible substrates using computer graphics and suggested that the ortho-substituted benzoic acids fit best in the active site...
  46. Kim S, Woo J, Hwang Y, Jeong D, Shin D, Kim K, et al. The tetrameric structure of Haemophilus influenza hybrid Prx5 reveals interactions between electron donor and acceptor proteins. J Biol Chem. 2003;278:10790-8 pubmed publisher
    ..Differences of electron donor binding surfaces in Prx proteins revealed by an analysis based on the structural information explain the electron donor specificities of various Prx proteins...
  47. Lehmann C, Pullalarevu S, Krajewski W, Willis M, Galkin A, Howard A, et al. Structure of HI0073 from Haemophilus influenzae, the nucleotide-binding domain of a two-protein nucleotidyl transferase. Proteins. 2005;60:807-11 pubmed
  48. Blanco J, Moore R, Faehnle C, Viola R. Critical catalytic functional groups in the mechanism of aspartate-beta-semialdehyde dehydrogenase. Acta Crystallogr D Biol Crystallogr. 2004;60:1808-15 pubmed
    ..However, small perturbations in the positioning of essential catalytic groups or reactive intermediates have dramatic effects on catalytic efficiency. ..
  49. Platonov A, Mironov K, Iatsyshina S, Koroleva I, Platonova O, Gushchin A, et al. [Multilocus sequence-typing for characterization of Moscow strains of Haemophilus influenzae type b]. Mol Gen Mikrobiol Virusol. 2003;:21-5 pubmed
    ..2 isolates from Norway and Sweden from among 7 foreign Hib strains studied up to now can be described as belonging to the same clonal complex; 5 Hib strains were different from the Russian ones. ..
  50. Chanyangam M, Smith A, Moseley S, Kuehn M, Jenny P. Contribution of a 28-kilodalton membrane protein to the virulence of Haemophilus influenzae. Infect Immun. 1991;59:600-8 pubmed
    ..We conclude that this 28-kDa outer membrane protein aids transepithelial invasion of type b strains but is not essential...
  51. Ahn H, Kim H, Yoon H, Lee B, Suh S, Yang J. Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition. EMBO J. 2003;22:2593-603 pubmed publisher
    ..The C-terminal domain shows structural similarity to trp repressor. We propose a plausible model for the TrmD(2)-tRNA(2) complex, which provides insights into recognition of the general tRNA structure by TrmD...
  52. Dastidar V, Mao W, Lomovskaya O, Zgurskaya H. Drug-induced conformational changes in multidrug efflux transporter AcrB from Haemophilus influenzae. J Bacteriol. 2007;189:5550-8 pubmed publisher
    ..We report that the reactivity of specific cysteines with FM is affected by the presence of some but not all substrates. Our results suggest that substrates induce conformational changes in AcrB(HI)...
  53. Harrington J, Wong S, Rosadini C, Garifulin O, Boyartchuk V, Akerley B. Resistance of Haemophilus influenzae to reactive nitrogen donors and gamma interferon-stimulated macrophages requires the formate-dependent nitrite reductase regulator-activated ytfE gene. Infect Immun. 2009;77:1945-58 pubmed publisher
    ..These results identify a requirement for FNR in positive control of ytfE and indicate a critical role for ytfE in resistance of H. influenzae to reactive nitrogen species and the antibacterial effects of macrophages...
  54. Flack F, Loosmore S, Chong P, Thomas W. The sequencing of the 80-kDa D15 protective surface antigen of Haemophilus influenzae. Gene. 1995;156:97-9 pubmed
    ..Here, the gene encoding D-15-Ag is shown to encode a 797-aa polypeptide which, after cleavage of the predicted signal peptide, would have a molecular mass of 85,632 Da...
  55. Cope L, Yogev R, Muller Eberhard U, Hansen E. A gene cluster involved in the utilization of both free heme and heme:hemopexin by Haemophilus influenzae type b. J Bacteriol. 1995;177:2644-53 pubmed
    ..These studies indicate that at least two and possible three gene products are required for utilization of heme bound to hemopexin by Hib strain DL42...
  56. Song X, Forsgren A, Janson H. The gene encoding protein D (hpd) is highly conserved among Haemophilus influenzae type b and nontypeable strains. Infect Immun. 1995;63:696-9 pubmed
    ..Protein D is 64 and 36% identical and 77 and 56% similar to the glycerophosphodiester phosphodiesterases (GlpQ) of Escherichia coli and Bacillus subtilis...
  57. van Ham S, van Alphen L, Mooi F, van Putten J. The fimbrial gene cluster of Haemophilus influenzae type b. Mol Microbiol. 1994;13:673-84 pubmed
    ..influenzae genome as a mobile virulence unit...
  58. Janson H, Ruan M, Forsgren A. Limited diversity of the protein D gene (hpd) among encapsulated and nonencapsulated Haemophilus influenzae strains. Infect Immun. 1993;61:4546-52 pubmed
    ..4-kbp DNA fragment upstream of the hpd gene that is absent in nontypeable strains. On the basis of the high degree of conservation of the hpd gene among H. influenzae strains, we conclude that protein D is a possible vaccine candidate...
  59. Soutourina J, Plateau P, Delort F, Peirotes A, Blanquet S. Functional characterization of the D-Tyr-tRNATyr deacylase from Escherichia coli. J Biol Chem. 1999;274:19109-14 pubmed
    ..In support of the idea of a general role of the D-Tyr-tRNATyr deacylase function in the detoxification of cells, similar genes can be recognized in Saccharomyces cerevisiae, Caenorhabditis elegans, Arabidopsis thaliana, mouse, and man...
  60. Sousa M, Trame C, Tsuruta H, Wilbanks S, Reddy V, McKay D. Crystal and solution structures of an HslUV protease-chaperone complex. Cell. 2000;103:633-43 pubmed
    ..When the complex forms, the carboxy-terminal helices of HslU distend and bind between subunits of HslV, and the apical helices of HslV shift substantially, transmitting a conformational change to the active site region of the protease...
  61. Horton N, Dorner L, Perona J. Sequence selectivity and degeneracy of a restriction endonuclease mediated by DNA intercalation. Nat Struct Biol. 2002;9:42-7 pubmed publisher
    ..Comparison of the HincII and EcoRV cocrystal structures suggests that sequence-dependent differences in base-stacking free energies are a crucial underlying factor mediating protein recognition by indirect readout...
  62. Lehmann C, Lim K, Toedt J, Krajewski W, Howard A, Eisenstein E, et al. Structure of 2C-methyl-D-erythrol-2,4-cyclodiphosphate synthase from Haemophilus influenzae: activation by conformational transition. Proteins. 2002;49:135-8 pubmed publisher
  63. Lim K, Zhang H, Tempczyk A, Krajewski W, Bonander N, Toedt J, et al. Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot. Proteins. 2003;51:56-67 pubmed publisher
    ..The substrate-binding domain is absent in HI0766 sequence family and may be provided by another Haemophilus influenzae partner protein, which is yet to be identified...
  64. Zhou J, Xu Z. Structural determinants of SecB recognition by SecA in bacterial protein translocation. Nat Struct Biol. 2003;10:942-7 pubmed publisher
    ..It also suggests a model of SecB-SecA interaction and its implication for the mechanism of pre-protein transfer in bacterial protein translocation...
  65. Morton D, Smith A, Ren Z, Madore L, VanWagoner T, Seale T, et al. Identification of a haem-utilization protein (Hup) in Haemophilus influenzae. Microbiology. 2004;150:3923-33 pubmed publisher
    ..The mutation also resulted in a reduced ability to utilize haem, haem-haemopexin, haem-albumin and haemoglobin-haptoglobin, thus identifying a general haem-utilization protein (Hup) in Haemophilus influenzae...
  66. Huang B, Vetting M, Roderick S. The active site of O-acetylserine sulfhydrylase is the anchor point for bienzyme complex formation with serine acetyltransferase. J Bacteriol. 2005;187:3201-5 pubmed publisher
    ..These results explain the partial inhibition of OASS by SAT on complex formation as well as the competitive dissociation of the complex by O-acetylserine...
  67. Parsons L, Lin F, Orban J. Peptidoglycan recognition by Pal, an outer membrane lipoprotein. Biochemistry. 2006;45:2122-8 pubmed publisher
    ..The structure provides insight into the mode of cell wall recognition for a broad class of Gram-negative membrane proteins, including OmpA and MotB, which have peptidoglycan-binding domains homologous to that of Pal...
  68. Meng G, Surana N, St Geme J, Waksman G. Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter. EMBO J. 2006;25:2297-304 pubmed publisher
    ..Overall, this study provides important insights into the mechanism of translocation in trimeric autotransporters...
  69. Felts R, Ou Z, Reilly T, Tanner J. Structure of recombinant Haemophilus influenzae e (P4) acid phosphatase reveals a new member of the haloacid dehalogenase superfamily. Biochemistry. 2007;46:11110-9 pubmed publisher
    ..This observation suggests a structure-based vaccine design strategy in which the dimer interface is disrupted in order to expose epitopes that are buried in dimeric P4...
  70. McCrea K, Watson W, Gilsdorf J, Marrs C. Identification of hifD and hifE in the pilus gene cluster of Haemophilus influenzae type b strain Eagan. Infect Immun. 1994;62:4922-8 pubmed
    ..These data indicate that the pepN homolog is not required for pilus biogenesis and that one end of the pilus gene cluster has been defined...
  71. Versalovic J, Koeuth T, Britton R, Geszvain K, Lupski J. Conservation and evolution of the rpsU-dnaG-rpoD macromolecular synthesis operon in bacteria. Mol Microbiol. 1993;8:343-55 pubmed
    ..However, MMS operon gene organization and cis-acting regulatory sequences appear to be conserved in diverse bacteria...
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    ..Extracts prepared from E. coli expressing recombinant protein D had an 8- to 22-fold-higher specific activity of glycerophosphodiester phosphodiesterase compared with extracts of E. coli not expressing protein D...
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    ..These results indicate that expression of a wild-type OxyR protein by H. influenzae is essential to allow this organism to protect itself against oxidative stresses in vitro...
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    ..Consistent with a role of Hi uvrA in DNA repair, a Hi uvrA mutant exhibited increased sensitivity of UV irradiation. Furthermore, Hi uvrA was able to complement a mutation in the E. coli uvrA locus...
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    ..The ModE-molybdate complex binds to DNA as a homodimer in E. coli and possibly in other organisms as well. In R. capsulatus, however, two ModE homologues (MopAB proteins) are required for repression...
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    ..These can undergo a second recombination reaction to generate odd-noded knots...
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    ..By analogy, YbaK may also play a role in nucleotide or oligonucleotide binding, the nature of which is yet to be determined...
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    ..The distribution of conserved residues suggests that the protein may work as a "molecular switch" triggered by ATP hydrolysis. The phylogenetic pattern of YjeE suggests its involvement in cell wall biosynthesis...
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    ..Based on these similarities, as well as having a comparable molecular surface to dsDNA, we propose that HI1450 may function as a dsDNA mimic in order to inhibit or regulate an as yet unidentified dsDNA binding protein...
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    ..Bacterial racemases, including glutamate racemase and DAP epimerase, are potential targets for the development of new agents effective against organisms resistant to conventional antibiotics...
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    ..The presence of two molybdate loci in H. influenzae suggests multiple transport systems for one substrate, with molABC constituting a low-affinity molybdate locus. ..
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