Genomes and Genes
Escherichia coli str. K-12 substr. MG1655
Alias: Escherichia coli MG1655, Escherichia coli str. K12 substr. MG1655, Escherichia coli str. MG1655, Escherichia coli strain MG1655
Publications127 found, 100 shown here
- Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterL M Guzman
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
J Bacteriol 177:4121-30. 1995..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
- Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantP P Cherepanov
Universitat Oldenburg, Germany
Gene 158:9-14. 1995..This system was applied in the construction of an E. coli endA deletion mutation which can be transduced by P1 to the genetic background of interest using TcR as a marker. The transductant can then be freed of the TcR if required...
- Molecular basis of bacterial outer membrane permeability revisitedHiroshi Nikaido
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
Microbiol Mol Biol Rev 67:593-656. 2003....
- An efficient recombination system for chromosome engineering in Escherichia coliD Yu
Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 97:5978-83. 2000..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
- Prokaryotic toxin-antitoxin stress response lociKenn Gerdes
Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
Nat Rev Microbiol 3:371-82. 2005..It has been proposed that toxin-antitoxin loci function in bacterial programmed cell death, but evidence now indicates that these loci provide a control mechanism that helps free-living prokaryotes cope with nutritional stress...
- The acetate switchAlan J Wolfe
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
Microbiol Mol Biol Rev 69:12-50. 2005..Next, the way in which cells regulate AMP-ACS activity through reversible acetylation is described. Finally, the "acetate switch" as it exists in selected eubacteria, archaea, and eukaryotes, including humans, is described...
- Multistability in the lactose utilization network of Escherichia coliErtugrul M Ozbudak
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nature 427:737-40. 2004..The phase diagram thus serves as a sensitive probe of molecular interactions and as a powerful tool for rational network design...
- The TetR family of transcriptional repressorsJuan L Ramos
Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
Microbiol Mol Biol Rev 69:326-56. 2005..Information related to the TetR family of regulators has been updated in a database that can be accessed at www.bactregulators.org...
- Two-component signal transductionA M Stock
Center for Advanced Biotechnology and Medicine and Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Annu Rev Biochem 69:183-215. 2000..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins...
- Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like proteinMaria A Schumacher
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
EMBO J 21:3546-56. 2002..Such binding suggests a mechanism for Hfq function...
- Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coliIris Keren
Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave, Boston, MA 02115
J Bacteriol 186:8172-80. 2004..The function of these specialized dormant cells is to ensure the survival of the population in the presence of lethal factors...
- Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivityHarald Weber
Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
J Bacteriol 187:1591-603. 2005..Thus, not only the expression of genes within a regulatory network but also the architecture of the network itself can be subject to regulation...
- Structural and mechanistic determinants of c-di-GMP signallingTilman Schirmer
Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
Nat Rev Microbiol 7:724-35. 2009..Here, we review recent data on the structure and functional properties of the protein families that are involved in c-di-GMP signalling and discuss the mechanistic implications...
- Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coliFranziska Mika
Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
Mol Microbiol 84:51-65. 2012..Thus, antagonistic regulation of csgD and RprA at the mRNA level integrates cell envelope stress signals with global gene expression during stationary phase and biofilm formation...
- The Y-family of DNA polymerasesH Ohmori
Mol Cell 8:7-8. 2001
- Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tractsByung Kwan Cho
Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA
Genome Res 18:900-10. 2008..These novel results provide new insight into how Fis modulates DNA topology at a genome scale and thus advance our understanding of the architectural bases of the E. coli nucleoid...
- Mechanisms and functions of DNA mismatch repairGuo Min Li
Graduate Center for Toxicology and Markey Cancer Center, Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536, USA
Cell Res 18:85-98. 2008....
- Optimality and evolutionary tuning of the expression level of a proteinErez Dekel
Department of Molecular Cell Biology and Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel
Nature 436:588-92. 2005..Thus, protein expression from the lac operon seems to be a solution of a cost-benefit optimization problem, and can be rapidly tuned by evolution to function optimally in new environments...
- A synthetic oscillatory network of transcriptional regulatorsM B Elowitz
Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
Nature 403:335-8. 2000..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
- SSB as an organizer/mobilizer of genome maintenance complexesRobert D Shereda
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
Crit Rev Biochem Mol Biol 43:289-318. 2008..Similar themes to those highlighted in this review are evident in all biological systems...
- Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coliClaude Sauter
European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nucleic Acids Res 31:4091-8. 2003..Finally, a detailed structural comparison shows that the Sm-fold is remarkably well conserved in bacteria, Archaea and Eukarya, and represents a universal and modular building unit for oligomeric RNA binding proteins...
- Bacterial persistence as a phenotypic switchNathalie Q Balaban
Laboratory of Living Matter and Center for Studies in Physics and Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Science 305:1622-5. 2004..Inherent heterogeneity of bacterial populations may be important in adaptation to fluctuating environments and in the persistence of bacterial infections...
- Translational control and target recognition by Escherichia coli small RNAs in vivoJohannes H Urban
Max Planck Institute for Infection Biology, RNA Biology Group, Chariteplatz 1, 10117 Berlin, Germany
Nucleic Acids Res 35:1018-37. 2007..We expect our GFP fusion approach to be applicable to sRNA targets of other bacteria, and also demonstrate that Vibrio RyhB sRNA represses a Vibrio sodB fusion when co-expressed in E.coli...
- DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxideM Zheng
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Bacteriol 183:4562-70. 2001..These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide...
- RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAsTeppei Morita
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
Genes Dev 19:2176-86. 2005..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
- The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNAToby J Soper
Program in Cellular, Molecular, Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, Maryland 21218 2685, USA
RNA 14:1907-17. 2008..Once base-pairing between DsrA and rpoS mRNA is established, interactions between Hfq and the mRNA may stabilize the RNA complex by removing Hfq from the sRNA...
- Insights into the replisome from the structure of a ternary complex of the DNA polymerase III alpha-subunitRichard A Wing
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
J Mol Biol 382:859-69. 2008..Finally, superimposing a recent structure of the clamp bound to DNA on this Pol IIIalpha complex with DNA places a loop of the beta-binding domain into the appropriate clamp cleft and supports a mechanism of polymerase switching...
- CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coliXin Wang
Department of Microbiology and Immunology, Emory University School of Medicine, 3105 Rollins Research Center, 1510 Clifton Road N E, Atlanta, GA 30322, USA
Mol Microbiol 56:1648-63. 2005..These studies define the crucial mechanisms, though not the only means, by which the Csr system influences biofilm formation...
- Effect of RyhB small RNA on global iron use in Escherichia coliEric Massé
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
J Bacteriol 187:6962-71. 2005..Our results demonstrate the broad effects of a single noncoding RNA on iron homeostasis...
- Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110Koji Hayashi
Division of Genome Dynamics, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi Pref, Japan
Mol Syst Biol 2:2006.0007. 2006..Errors in the original MG1655 sequence (<1 per 13,000 bases) were mostly within portions sequenced with out-dated technology based on radioactive chemistry...
- Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coliChristina Kahramanoglou
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
Nucleic Acids Res 39:2073-91. 2011..Our study serves as a proof-of-principle for the use of ChIP-seq for global DNA-binding proteins in bacteria, which should become significantly more economical and feasible with the development of multiplexing techniques...
- Structure of the SSB-DNA polymerase III interface and its role in DNA replicationAimee H Marceau
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706 1532, USA
EMBO J 30:4236-47. 2011..Destabilization of the χ/SSB complex in vivo produces cells with temperature-dependent cell cycle defects that appear to arise from replisome instability...
- The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coliUwe Sauer
Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
J Biol Chem 279:6613-9. 2004..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism?..
- Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAsPeter J Mikulecky
Department of Chemistry, Indiana University, 800 E Kirkwood Avenue, Bloomington, Indiana 47405, USA
Nat Struct Mol Biol 11:1206-14. 2004..Our model explains how Hfq can simultaneously bind a ncRNA and its mRNA target to facilitate the strand displacement reaction required for Hfq-dependent translational regulation...
- Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotesDeo Prakash Pandey
Department of Biochemistry and Molecular Biology, University of Southern Denmark DK 5230 Odense M, Denmark
Nucleic Acids Res 33:966-76. 2005....
- Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coliTao Wu
Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
Cell 121:235-45. 2005..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
- N6-methyl-adenine: an epigenetic signal for DNA-protein interactionsDidier Wion
INSERM U318, CHU Michallon, Universite Joseph Fourier, 38043 Grenoble, France
Nat Rev Microbiol 4:183-92. 2006..In alpha-proteobacteria, CcrM methylation regulates the cell cycle in Caulobacter, Rhizobium and Agrobacterium, and has a role in Brucella abortus infection...
- Persisters: a distinct physiological state of E. coliDevang Shah
Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave, Boston, MA 02115, USA
BMC Microbiol 6:53. 2006..Recent progress in understanding persisters is encouraging, but the main obstacle in understanding their nature was our inability to isolate these elusive cells from a wild-type population since their discovery in 1944...
- The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stabilityR Lange
Department of Biology, University of Konstanz, Germany
Genes Dev 8:1600-12. 1994....
- AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutantsH Okusu
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
J Bacteriol 178:306-8. 1996..This experiment identified the AcrAB system as the major pump responsible for making the Mar mutants resistant to many agents, including tetracycline, chloramphenicol, ampicillin, nalidixic acid, and rifampin...
- Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coliB L Wei
Department of Molecular Biology and Immunology, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Blvd, Fort Worth, TX 76107 2699, USA
Mol Microbiol 40:245-56. 2001..Thus, CsrA stimulates flhDC gene expression by a post-transcriptional mechanism reminiscent of its function in the repression of glycogen biosynthesis...
- DNA as a nutrient: novel role for bacterial competence gene homologsS E Finkel
Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 1340, USA
J Bacteriol 183:6288-93. 2001..Homologs of these E. coli genes are present in many members of the gamma subclass of Proteobacteria, suggesting that the mechanisms for consumption of DNA may have been widely conserved during evolution...
- The HtrA family of proteases: implications for protein composition and cell fateTim Clausen
Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, 82152 Martinsried, Germany
Mol Cell 10:443-55. 2002..Here, we discuss the properties and roles of this ATP-independent protease chaperone system in protein metabolism and cell fate...
- (p)ppGpp: still magical?Katarzyna Potrykus
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2785, USA
Annu Rev Microbiol 62:35-51. 2008..Many basic questions still exist. This review tries to focus on some issues that linger even for the most widely characterized bacterial strains...
- Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in checkNam Ky Tonthat
Department of Biochemistry and Molecular Biology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
EMBO J 30:154-64. 2011..Thus, the combined data reveal how SlmA derails Z-ring formation at the correct place and time to effect NO...
- Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coliHiroshi Ogasawara
Department of Frontier Bioscience, Hosei University, Tokyo, Japan
FEMS Microbiol Lett 312:160-8. 2010..The possible interplay between three activators was analysed in detail...
- A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutatorS Altuvia
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 90:43-53. 1997..Our results suggest that the oxyS RNA acts as a regulator that integrates adaptation to hydrogen peroxide with other cellular stress responses and helps to protect cells against oxidative damage...
- GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motilityRoger Simm
Microbiology and Tumorbiology Center MTC, Karolinska Institutet, Box 280, SE 171 77 Stockholm, Sweden
Mol Microbiol 53:1123-34. 2004..Thus, the data suggest that c-di-GMP is a novel global second messenger in bacteria the metabolism of which is controlled by GGDEF and EAL domain proteins...
- DNA mismatch repairThomas A Kunkel
Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Annu Rev Biochem 74:681-710. 2005..Emphasis is on structure-function studies of MMR proteins, on how mismatches are recognized, on the process by which the newly replicated strand is identified, and on excision of the replication error...
- Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coliChristopher J Cardinale
Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA
Science 320:935-8. 2008..Deletion of the cryptic rac prophage in wild-type E. coli increases bicyclomycin resistance and permits deletion of nusG. Thus, Rho termination, supported by NusA and NusG, is required to suppress the toxic activity of foreign genes...
- Structural basis for the regulated protease and chaperone function of DegPTobias Krojer
Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
Nature 453:885-90. 2008..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
- Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coliChristina Pesavento
Institut für Biologie Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
Genes Dev 22:2434-46. 2008..In summary, these data reveal the logic and sequence of molecular events underlying the motile-to-adhesive "lifestyle" switch in E. coli...
- Structural analysis of a monomeric form of the twin-arginine leader peptide binding chaperone Escherichia coli DmsDCharles M Stevens
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
J Mol Biol 389:124-33. 2009..coli DmsA and the most conserved regions on the surface of EcDmsD...
- Structural analysis of substrate binding by the TatBC component of the twin-arginine protein transport systemMichael J Tarry
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
Proc Natl Acad Sci U S A 106:13284-9. 2009..Similar TatBC-substrate complexes can be generated by an alternative in vitro reconstitution method and using a different substrate protein...
- Remnant signal peptides on non-exported enzymes: implications for the evolution of prokaryotic respiratory chainsBérengère Ize
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Microbiology 155:3992-4004. 2009..These data highlight an intimate genetic and evolutionary link between some non-exported redox enzymes and those transported across membranes by the Tat translocation system...
- Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelopeJustyna Sawa
Institute of Molecular Pathology, A 1030 Vienna, Austria
J Biol Chem 286:30680-90. 2011..Furthermore, our in vitro and in vivo data imply a pH-related function of DegQ in the bacterial cell envelope...
- The single-stranded DNA-binding protein of Escherichia coliR R Meyer
Department of Biological Sciences, University of Cincinnati, Ohio 45221
Microbiol Rev 54:342-80. 1990..During recombination, SSB interacts with the RecBCD enzyme to find Chi sites, promotes binding of RecA protein, and promotes strand uptake...
- A large-conductance mechanosensitive channel in E. coli encoded by mscL aloneS I Sukharev
Laboratory of Molecular Biology, University of Wisconsin Madison 53706
Nature 368:265-8. 1994..The mscL nucleotide sequence predicts a unique protein of only 136 amino acids, with a highly hydrophobic core and very different from porins or other known proteins...
- Coordinated regulation of amino sugar-synthesizing and -degrading enzymes in Escherichia coli K-12J A Plumbridge
Institut de Biologie Physico chimique URA1139, Paris, France
J Bacteriol 175:4951-6. 1993....
- A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32J Gamer
Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
EMBO J 15:607-17. 1996..These data indicate that reversible inhibition of sigma32 activity through transient association of DnaK and DnaJ is a central regulatory element of the heat shock response...
- The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coliBijoy K Mohanty
Department of Genetics, University of Georgia, Athens, GA 30602, USA
Mol Microbiol 54:905-20. 2004..Analysis of mRNA half-lives in hfq, deltapcnB and hfq deltapcnB mutants suggests that Hfq and PAP I function in the same mRNA decay pathway...
- Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhBTaras Afonyushkin
Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter Dr Bohrgasse 9 4, A 1030 Vienna, Austria
Nucleic Acids Res 33:1678-89. 2005..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover...
- A switch from high-fidelity to error-prone DNA double-strand break repair underlies stress-induced mutationRebecca G Ponder
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell 19:791-804. 2005..Our findings reveal an RpoS-controlled switch from high-fidelity to mutagenic DSBR under stress. This limits genetic instability both in time and to localized genome regions, potentially important evolutionary strategies...
- Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitchAlexander Serganov
Nature 441:1167-71. 2006..In addition, this structure provides insight into how folded RNAs can form precision binding pockets that rival those formed by protein genetic factors...
- The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone poolMartijn Bekker
Swammerdam Institute for Life Sciences Molecular Microbial Physiology, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
J Bacteriol 192:746-54. 2010..coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity...
- In situ protein folding and activation in bacterial inclusion bodiesNuria Gonzalez-Montalban
Institute for Biotechnology and Biomedicine, Department of Genetics and Microbiology, Autonomous University of Barcelona and CIBER BBN Network in Bioengineering, Biomaterials and Nanomedicine, Bellaterra, 08193 Barcelona, Spain
Biotechnol Bioeng 100:797-802. 2008..In addition, in situ folding and protein activation in inclusion bodies is negatively regulated by the chaperone DnaK...
- The mechanisms of carbon catabolite repression in bacteriaJosef Deutscher
Laboratorie de Microbiologie et Génétique Moléculaire, INRA AgroParisTech CNRS, F 78850 Thiverval Grignon, France
Curr Opin Microbiol 11:87-93. 2008..PTS-independent CCR mechanisms are operative in several other bacteria...
- DNA apurinic-apyrimidinic site binding and excision by endonuclease IVElsa D Garcin
Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, MB4 La Jolla, California 92037, USA
Nat Struct Mol Biol 15:515-22. 2008....
- Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coliPaola Sperandeo
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy
J Bacteriol 190:4460-9. 2008..Moreover, the location of at least one of these five proteins in every cellular compartment suggests a model for how the LPS assembly pathway is organized and ordered in space...
- Protein exchange dynamics at chemoreceptor clusters in Escherichia coliSonja Schulmeister
Zentrum für Molekulare Biologie and Interdisziplinäres Zentrum für Wissenschaftliches Rechnen, Universitat Heidelberg, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 105:6403-8. 2008..Our results indicate that the rates of protein turnover at the cluster have evolved to ensure optimal performance of the chemotaxis pathway...
- Fold and function of polypeptide transport-associated domains responsible for delivering unfolded proteins to membranesTimothy J Knowles
Cancer Research UK Institute for Cancer Studies, School of Medicine, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
Mol Microbiol 68:1216-27. 2008..Together, this provides the first structural model of how multiple POTRA domains recruit substrates from the periplasmic solution into the outer membrane...
- Determination of the number of active GroES subunits in the fused heptamer GroES required for interactions with GroELTatsuya Nojima
Chemical Resources Laboratory R1 7, Tokyo Institute of Technology, Yokohama, Japan
J Biol Chem 283:18385-92. 2008..These results indicate the presence of an intermediate GroEL/substrate/GroES complex in which the substrate and GroES bind to GroEL by sharing seven common binding sites...
- Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNACai Guang Yang
Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA
Nature 452:961-5. 2008..In addition, the first crystal structure of ABH2, presented here, provides a structural basis for designing inhibitors of this human DNA repair protein...
- Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) RecombinationRalf Heermann
Ludwig Maximilians Universitat Munchen, Department Biologie I, Bereich Mikrobiologie, Maria Ward Str, 1a, D 80638 München, Germany
Microb Cell Fact 7:14. 2008..For many investigations, chromosomal systems are required rather than artificial plasmid based systems...
- Spatial regulators for bacterial cell division self-organize into surface waves in vitroMartin Loose
Biotechnologisches Zentrum der Technischen Universität Dresden, Tatzberg 47 51, 01307 Dresden, Germany
Science 320:789-92. 2008..We present a reaction-diffusion model of the MinD and MinE dynamics that accounts for our experimental observations and also captures the in vivo oscillations...
- Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin moduleGuang Yao Li
Division of Signaling Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
J Mol Biol 380:107-19. 2008....
- Messenger RNA interferase RelE controls relBE transcription by conditional cooperativityMartin Overgaard
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
Mol Microbiol 69:841-57. 2008..A mutational analysis of the operator sites showed that RelE in excess counteracted cooperative binding of the RelB(2)*RelE complexes to the operator sites. Thus, RelE controls relBE transcription by conditional cooperativity...
- Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrateS Meyer
Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, Dortmund 44227, Germany
J Mol Biol 380:532-47. 2008..We propose a model for the interaction between GidA and MnmE, which is supported by site-directed mutagenesis. Our data suggest that this interaction is modulated and potentially regulated by the switch function of the G domain of MnmE...
- Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoterDouglas F Browning
School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
J Bacteriol 190:7258-67. 2008..Differences in the upstream IHF and Fis binding sites at the nir promoter in related enteric bacteria fix the level of nir operon expression under anaerobic growth conditions...
- GadX/GadW-dependent regulation of the Escherichia coli acid fitness island: transcriptional control at the gadY-gadW divergent promoters and identification of four novel 42 bp GadX/GadW-specific binding sitesAngela Tramonti
Istituto di Biologia e Patologia Molecolari, CNR, Sapienza Universita di Roma, Roma, Italy
Mol Microbiol 70:965-82. 2008..The presence of five GadX/GadW-specific binding sites in the AFI suggests that GadX and GadW may act as H-NS counter-silencers...
- CDD: specific functional annotation with the Conserved Domain DatabaseAron Marchler-Bauer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bldg 38 A, Room 8N805, 8600 Rockville Pike, Bethesda, MD 20894, USA
Nucleic Acids Res 37:D205-10. 2009....
- Molecular basis for regulation of the heat shock transcription factor sigma32 by the DnaK and DnaJ chaperonesFernanda Rodriguez
Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
Mol Cell 32:347-58. 2008....
- Transforming DNA uptake gene orthologs do not mediate spontaneous plasmid transformation in Escherichia coliDongchang Sun
Centre National de la Recherche Scientifique, LMGM UMR5100, F 31000 Toulouse, France
J Bacteriol 191:713-9. 2009..Our data together, with previous reports that HofQ is required for the use of dsDNA as a carbon source, suggest the existence of two routes for DNA entry, at least across the outer membrane of E. coli...
- Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell deathMichael A Kohanski
Department of Biomedical Engineering, Center for BioDynamics, and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
Cell 135:679-90. 2008....
- PSICIC: noise and asymmetry in bacterial division revealed by computational image analysis at sub-pixel resolutionJonathan M Guberman
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Comput Biol 4:e1000233. 2008....
- Role of the sRNA GcvB in regulation of cycA in Escherichia coliSarah C Pulvermacher
Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Microbiology 155:106-14. 2009..However, mutations predicted to disrupt base-pairing between cycA mRNA and GcvB did not alter expression of cycA : : lacZ. A model for GcvB function in cell physiology is discussed...
- Contact-dependent growth inhibition causes reversible metabolic downregulation in Escherichia coliS K Aoki
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106, USA
J Bacteriol 191:1777-86. 2009..Consistent with the observed decrease in Deltap, the phage shock response was induced in cells undergoing CDI but not in recovering cells, based on analysis of levels of pspA mRNA...
- FtsZ condensates: an in vitro electron microscopy studyDavid Popp
ERATO Actin Filament Dynamics Project, Japan Science and Technology Corporation, c o RIKEN Harima Institute at Spring 8, 1 1 1 Kouto, Sayo, Hyogo 679 5148, Japan
Biopolymers 91:340-50. 2009..We shed light on the molecular arrangement of FtsZ filaments within these suprastructures using high resolution electron microscopy...
- The oligomerization of OxyR in Escherichia coliGwendowlyn S Knapp
Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, Texas 77843 2128, USA
Protein Sci 18:101-7. 2009..We compared the properties of these hot spots to those described in the literature from other systems. The hot spots identified in this study are not especially conserved amongst a set of OxyR orthologs...
- Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNACatherine Joce
School of Chemistry, University of Leeds, Leeds, UKLS2 9JT, UK
Org Biomol Chem 7:635-8. 2009..The efficient synthesis of a range of stable SAM mimetics, and their ability to promote the binding of the E. coli methionine repressor (MetJ) to its operator DNA, is described...
- Maturation and degradation of ribosomal RNA in bacteriaMurray P Deutscher
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA
Prog Mol Biol Transl Sci 85:369-91. 2009..The second focus of this review is to describe these degradative reactions, the RNases that carry them out, and the conditions that initiate the turnover process...
- Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality controlQing Tao Shen
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
Proc Natl Acad Sci U S A 106:4858-63. 2009..Our findings imply that DegP might regulate its dual roles during protein quality control, depending on its assembly state in the narrow bacterial envelope...
- DinB upregulation is the sole role of the SOS response in stress-induced mutagenesis in Escherichia coliRodrigo S Galhardo
Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030 3411, USA
Genetics 182:55-68. 2009....
- Kinetic behavior of the major multidrug efflux pump AcrB of Escherichia coliKeiji Nagano
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 106:5854-8. 2009..For the very hydrophilic cefazolin there was little sign of efflux...
- Specific genomic sequences of E. coli promote replicational initiation by directly reactivating ADP-DnaAKazuyuki Fujimitsu
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812 8582, Japan
Genes Dev 23:1221-33. 2009..Our findings reveal a novel regulatory pathway that promotes the initiation of chromosomal replication via DnaA reactivation...
- The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinDBang Shen
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
Mol Microbiol 72:410-24. 2009..This binding displaces FtsA and/or ZipA, and more importantly, positions MinC(N) near the FtsZ polymers making it a more effective inhibitor...
- Multiple chaperonins in bacteria--why so many?Peter A Lund
School of Biosciences, University of Birmingham, Birmingham, UK
FEMS Microbiol Rev 33:785-800. 2009....
- The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicinsYing Zhang
Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
Mol Microbiol 75:623-36. 2010..coli cells...
- A conserved haem redox and trafficking pathway for cofactor attachmentCynthia L Richard-Fogal
Department of Biology, Washington University, St Louis, MO 63130, USA
EMBO J 28:2349-59. 2009....
- Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabBYoujun Feng
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
J Biol Chem 284:29526-35. 2009..These data indicate that the key reaction in UFA synthesis catalyzed by FabB is elongation of the cis-3-decenoyl-ACP produced by FabA...
- Induction kinetics of a conditional pH stress response system in Escherichia coliGeorg Fritz
Arnold Sommerfeld Center for Theoretical Physics, Ludwig Maximilians Universitat Munchen, Germany
J Mol Biol 393:272-86. 2009..Furthermore, the analysis puts causal constraints on the precise mechanism of signal transduction via the regulatory protein CadC...
- GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLIValley J Stewart; Fiscal Year: 2010..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
- Novel Approaches for the Control of Microbial PathogensJOSEPH F LUTKENHAUS; Fiscal Year: 2010..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..
- Glucose Signaling: Regulation of Enzyme ActivityDONALD PETTIGREW; Fiscal Year: 2006..coli. ..
- IMP Dehydrogenase and the Hydra of Cancer ChemotherapyGEORGE MARKHAM; Fiscal Year: 2008..These results will reveal the inner working of an essential enzyme and whether there is promise for enhancing clinical efficacy through combinations of known drugs or the discovery of novel subdomain ligands. ..
- SCREEN DEVELOPMENT FOR ANTIMICROBIAL PRODRUGSKim Lewis; Fiscal Year: 2009..RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
- BASIC MECHANISMS OF ION CHANNEL FUNCTIONChristopher Miller; Fiscal Year: 2009..abstract_text> ..
- The Nature of Bacterial UncultivabilityKim Lewis; Fiscal Year: 2008..Unculturable bacteria are an enormous untapped source of potentially useful pharmaceutical compounds. Unculturable bacteria also make up most of the human oral and intestinal microflora. [unreadable] [unreadable] [unreadable]..
- Structural studies on the Initiator protein, IBP39.Maria Schumacher; Fiscal Year: 2008..vaginalis suggests it is a possible target for the treatment of trichomoniasis, which afflicts over 170 million individuals each _,ear, ..
- MECHANISMS OF CHAPERONE FUNCTION IN THE LENSHassane McHaourab; Fiscal Year: 2008..Reporter group spectroscopic approaches will be used to provide insight into the structural basis of recognition and binding. ..
- X-Ray Crystallographic Studies of N4 RNA PolymerasesKatsuhiko Murakami; Fiscal Year: 2008..These studies will provide insights into the mechanism of transcription of the T7 bacteriophage-like single-subunit RNAP family, and specifically those enzymes in the family that require accessory factors. ..
- MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORSDeepak Bastia; Fiscal Year: 2008..abstract_text> ..
- Phages of Burkholderia Cepacia: Biology and TherapeuticsRyland Young; Fiscal Year: 2009..This project seeks to explore the use of these natural antibacterial agents to address this critical public health need. ..
- Engineering Large scale pathways from organisms to Escherichia coliJames Liao; Fiscal Year: 2008..In particular, selected projects proposed here will be used as modules in a workshop established by FRT at Ohio State and form the basis for a course (Metabolic Engineering) at UCLA. ..
- GENE CONTROL IN INFECTION AND LYSOGENY BY PHAGE LAMBDAJeffrey Roberts; Fiscal Year: 2008..We will investigate the mechanism of transcription termination and the manner in which regulatory proteins interfere with this process. ..
- SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETINGRobert Stroud; Fiscal Year: 2007..These will instruct in the mechanism of replacing the role of RNA by protein, and therefore in the essence of the dynamic roles of SRP RNA and cpSRP43. ..
- OmpR and acid regulation of Escherichia coli fim genesWILLIAM SCHWAN; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- Plasmid-Mediated Quinolone ResistanceGeorge Jacoby; Fiscal Year: 2007..unreadable] [unreadable]..
- Non-natural Nucleoside Inhibitors of DNA PolymerasesAnthony Berdis; Fiscal Year: 2009..This knowledge will facilitate the development of new drugs as the repertoire of targets becomes more differentiated. ..
- Proton-coupled electron translocation in metalloenzymesROBERT CUKIER; Fiscal Year: 2006..Contemporaneously, we will explore PTR by simulation methodologies that we are developing, which combine quantum mechanics and molecular dynamics to quantitatively describe proton translocation. ..
- Biochemistry of Phenazine Production in Pseudomonas aeruginosaJames Parsons; Fiscal Year: 2009..New strategies that complement existing antimicrobial therapies clearly need to be developed to more effectively combat P. aeruginosa infections. ..
- TETHERED DOMAINS AS REGULATORY ELEMENTSGregory Grant; Fiscal Year: 2009..Our observations that PGDH from M. tuberculosis is inhibited by L-serine and that PGDH from humans is not, may provide a new focus in drug development for treatment of tuberculosis. ..
- Structural studies on the P1 plasmid partition apparatusMaria Schumacher; Fiscal Year: 2009....
- The structural basis for APP cleavabilityYa Ha; Fiscal Year: 2009..abstract_text> ..
- Chemogenomic Analysis of E. coli Response to NO speciesJames C Liao; Fiscal Year: 2010..If hew TF targets are predicted, then the iteration continues. Otherwise, the predicted networks are verified using genetic and biochemical experiments. ..
- Fatty Acid Biosynthesis in Cryptosporidium parvumGuan Zhu; Fiscal Year: 2010..3) To validate that fatty acid metabolic enzymes may serve as rational drug target in Cryptosporidium by discovering inhibitors selectively against, parasite fatty acid metabolic enzymes. ..
- Nitric Oxide Interaction with Red Blood CellsJames C Liao; Fiscal Year: 2010..Specific aim 2 will focus on the functional roles of this regulation using isolated porcine pulmonary and coronary microvessels. Together, these results will suggest clinical relevance and potential interventions. ..
- Metabolomics of the Virus-host Cell InteractionJoshua D Rabinowitz; Fiscal Year: 2010..Such pathways, once identified, will be attractive new targets for antiviral therapy. ..
- Structural mechanism of DNA segregation by the pSK41 par systemMaria Schumacher; Fiscal Year: 2010..Thus, understanding the structural basis for DNA segregation by this par system will provide several points of potential therapeutic intervention against pSK41 harboring multidrug resistant S. aureus strains. ..
- The Electrochemistry of Diheme Cytochrome c PeroxidasesSean J Elliott; Fiscal Year: 2010..Further, our study of triheme CCPs will elucidate the CCP machinery which is unique to pathogens such as Salmonella enterica and Yersinia pestis, providing new insights into their biochemical pathways. ..
- TERMINAL ENZYMES OF HEME SYNTHESISHarry A Dailey; Fiscal Year: 2010..3.) identify and characterize protein-protein interactions between ferrochelatase and PPO. ..
- MECHANISM OF TERMINATION OF DNA REPLICATIONDeepak Bastia; Fiscal Year: 2010..Finally, we wish to uncover the mode of action of the Swi1 and Swi3 proteins to determine if these act as "antisweepases". ..
- PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE ITOMOKO NONE OHNISHI; Fiscal Year: 2010..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
- Chemoreception and Signal AmplificationMICHAEL MANSON; Fiscal Year: 2005..The supramolecular architecture of the receptor patch and its associated proteins will be examined using electron tomography. ..
- REGULATION OF PYRIMIDINE GENE EXPRESSION IN BACTERIACHARLES TURNBOUGH; Fiscal Year: 2001..Although these studies are done with E. coli, the work is likely to be applicable to the study of gene expression and regulation in all bacteria and probably in eukaryotes, as well. ..
- Studying GrpE: Protein Function, Stability, and FoldingANDREW MEHL; Fiscal Year: 2002..Point mutants will also be created to test the function of salt bridges within the dimer interface. An internal deletion mutant is proposed that will potentially lead to only a monomer of GrpE. ..
- Proline uptake in staphylococcus aureus pathogenesisWILLIAM SCHWAN; Fiscal Year: 2002..The results of this study will help us understand the role of proline transport in S. aureus infections. ..
- NATURAL SUBSTRATES AND INHIBITORS OF MICROBIAL MDR PUMPSKim Lewis; Fiscal Year: 2002..MDR inhibitors will potentiate the action of conventional antibiotics, aiding eradication of multidrug resistant human pathogens. ..
- STRUCTURAL & ENERGETIC PRINCIPLES OF MEMBRANE PROTEINSKAREN FLEMING; Fiscal Year: 2002....
- Zeiss LSM510 META confocal-fluorescence spectroscopyHAROLD ERICKSON; Fiscal Year: 2003..Projects for this module are proposed by two faculty from Cell Biology and two from Biochemistry. This will be the only FCS facility at Duke University, and we expect additional projects from across the campus. ..
- Contribution of DNA photoproducts to UV mutagenesisDouglas Fix; Fiscal Year: 2003..Furthermore, these AREA-funded studies will provide valuable research experience for undergraduate students in the biological sciences. ..
- MECHANISM OF E. COLI TERMINATION FACTOR RHOBarbara Stitt; Fiscal Year: 2003..The results will provide a better understanding of how the energy of ATP hydrolysis can be transduced to movement. ..
- CHARACTERIZATION OF B ANTHRACIS EXOSPORIUM PROTEINSCHARLES TURNBOUGH; Fiscal Year: 2004..anthracis spore surface (i.e., exosporium) to create affinity-matured single chain antibodies that neutralize exosporium protein activity, then test the effects of these reagents on spore properties. ..
- Cryptosporidium parvum DNA replication proteinsGuan Zhu; Fiscal Year: 2004..The completion of these aims will not only prove or refute our hypothesis, but also establish a new foundation for the drug discovery targeting the parasite unique DNA mechanism machinery. ..
- Bacterial Cell Surfaces Gordon ConferenceBarry Rosen; Fiscal Year: 2004..abstract_text> ..
- Transport and Metabolism of Folate Analogs in E. coliJACALYN GREEN; Fiscal Year: 2004..coli, especially with regard to a newly identified transport protein AbgT. ..
- STRUCTURE BASED DRUG DESIGNRobert Stroud; Fiscal Year: 2004..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..
- Nucleic Acid Gordon Research ConferenceANNA PYLE; Fiscal Year: 2004..Poster sessions and optional recreational activities will stimulate informal interactions among participants. ..
- THYMIDYLATE SYNTHASERobert Stroud; Fiscal Year: 2001..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
- NOVEL METHODS FOR DISCOVERY OF ANTI-MICROBIALSKim Lewis; Fiscal Year: 2005..Determination of chemical structure will be performed by a combination of MS and NMR methods. ..
- BIODEFENSE THERAPEUTICS FROM UNCULTURED MICROORGANISMSKim Lewis; Fiscal Year: 2005..Determination of chemical structure will be performed by a combination of MS and NMR methods. ..
- Development of a HTS system:topoisomerase targets (RMI)Yuk Ching Tse Dinh; Fiscal Year: 2004..The screening system should also be applicable for targeting topoisomerases in pathogenic bacteria relevant for biodefense. ..
- ASM Conference on the New Phage BiologyRyland Young; Fiscal Year: 2004..The site was chosen to minimize costs, facilitate travel arrangements, and to foster an interactive atmosphere which will break down disciplinary borders. ..
- 2005 Mechanisms of Membrane Transport Gordon ConferenceRobert Stroud; Fiscal Year: 2005..abstract_text> ..
- Molecular Basis for Transmembrane Conduction & SignalingRobert Stroud; Fiscal Year: 2006..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
- Automated Chip-Based Metabolomic AnalysisJames Liao; Fiscal Year: 2006..abstract_text> ..
- Molecular mechanism of antibiotic rifampicin actionIrina Artsimovitch; Fiscal Year: 2006..Finally a set of rules for design of more potent rifamycin-like antibiotics will be formulated to achieve the long-term goal of knowledge-based creation of the new generation of antibacterial drugs. ..
- Substrate Recognition of a tRNA Modifying EnzymeGeorge Garcia; Fiscal Year: 2006..A clear understanding of the similarities and differences between the TGTs from eubacteria and human will be of significance for many reasons but most obviously for the future design of novel antibiotics. ..
- MUROPEPTIDE RECYCLING PATHWAY & BETA LACTAMASE INDUCTIONJames Park; Fiscal Year: 2006..unreadable] [unreadable]..
- Single Molecule Studies of DNA HelicasesPIERO BIANCO; Fiscal Year: 2007..abstract_text> ..
- Migraine Headache Treatment by Drug Aerosol InhalationJoshua Rabinowitz; Fiscal Year: 2004..Accomplishment of the goals of Phase II will lead directly to human clinical testing of a commercially viable device that could improve the lives of millions of people every year. ..
- ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFERPERRY FREY; Fiscal Year: 2006..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
- ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISMGEORGE MARKHAM; Fiscal Year: 2008..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
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