Escherichia coli O157:H7 str. Sakai

Summary

Alias: Escherichia coli O157:H7 strain Sakai

Top Publications

  1. Srimanote P, Paton A, Paton J. Characterization of a novel type IV pilus locus encoded on the large plasmid of locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infect Immun. 2002;70:3094-100 pubmed
    ..Homologues of the pO113 pil locus were present in several other LEE-negative STEC strains but not in LEE-positive STEC strains belonging to serogroup O26, O111, or O157. ..
  2. Lathem W, Grys T, Witowski S, Torres A, Kaper J, Tarr P, et al. StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor. Mol Microbiol. 2002;45:277-88 pubmed
    ..Cleavage of C1-INH by StcE could plausibly cause localized pro-inflammatory and coagulation responses resulting in tissue damage, intestinal oedema and thrombotic abnormalities. ..
  3. Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G. Solution structure of the R3H domain from human Smubp-2. J Mol Biol. 2003;326:217-23 pubmed
    ..A strong correlation between conservation of hydrophobic amino acids and side-chain solvent protection indicates that the structure of the Smubp-2 R3H domain is representative of R3H domains in general. ..
  4. Lathem W, Bergsbaken T, Witowski S, Perna N, Welch R. Acquisition of stcE, a C1 esterase inhibitor-specific metalloprotease, during the evolution of Escherichia coli O157:H7. J Infect Dis. 2003;187:1907-14 pubmed
    ..coli 1 complex in a stepwise manner. These data refine the sequential model of E. coli O157:H7 evolution proposed elsewhere. ..
  5. Batisson I, Guimond M, Girard F, An H, Zhu C, Oswald E, et al. Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli. Infect Immun. 2003;71:4516-25 pubmed
    ..These observations suggest that Paa contributes to the early stages of A/E E. coli virulence. ..
  6. Cantarelli V, Kodama T, Nijstad N, Abolghait S, Nada S, Okada M, et al. Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu/TccP. Cell Microbiol. 2007;9:1782-95 pubmed
    ..Binding of cortactin to these effectors is dependent upon tyrosine phosphorylation and a balance between tyrosine phosphorylation and dephosphorylation of cortactin is required to regulate pedestal formation by EHEC. ..
  7. Ostheimer G, Barkan A, Matthews B. Crystal structure of E. coli YhbY: a representative of a novel class of RNA binding proteins. Structure. 2002;10:1593-601 pubmed
    ..Taken together, the evidence suggests that members of UPF0044 constitute a previously unrecognized class of RNA binding domain...
  8. Kim J, Nietfeldt J, Ju J, Wise J, Fegan N, Desmarchelier P, et al. Ancestral divergence, genome diversification, and phylogeographic variation in subpopulations of sorbitol-negative, beta-glucuronidase-negative enterohemorrhagic Escherichia coli O157. J Bacteriol. 2001;183:6885-97 pubmed publisher
    ..DNA sequence analysis of representative polyphyletic markers showed that genome diversity accrued through random drift and bacteriophage-mediated events...
  9. Chander M, Raducha Grace L, Demple B. Transcription-defective soxR mutants of Escherichia coli: isolation and in vivo characterization. J Bacteriol. 2003;185:2441-50 pubmed
    ..This work begins the general identification of the residues in the SoxR polypeptide that are critical for transducing oxidative stress signals into gene activation. ..

More Information

Publications189 found, 100 shown here

  1. Diez A, Tunlid A, Nystrom T. The Escherichia coli ftsK1 mutation attenuates the induction of sigma(S)-dependent genes upon transition to stationary phase. FEMS Microbiol Lett. 2002;206:19-23 pubmed
    ..Thus, a mutation in the cell division gene, ftsK, uncouples the otherwise coordinated induction of sigma(S)-dependent genes and the universal stress response gene, uspA, during entry into stationary phase. ..
  2. Wang L, Huskic S, Cisterne A, Rothemund D, Reeves P. The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene. J Bacteriol. 2002;184:2620-5 pubmed
    ..Genes outside the recombination sites are 99.6 to 100% identical in the two clones, while most genes thought to have transferred with the O157 gene cluster are 95 to 98% identical...
  3. Liu Z, Tek V, Akoev V, Zolkiewski M. Conserved amino acid residues within the amino-terminal domain of ClpB are essential for the chaperone activity. J Mol Biol. 2002;321:111-20 pubmed
  4. Akerlund T, Gullbrand B, Nordstr m K. Effects of the Min system on nucleoid segregation in Escherichia coli. Microbiology. 2002;148:3213-22 pubmed publisher
    ..It is also shown how the disturbed nucleoid segregation can explain in part the unexpected finding that the clear majority of cells in min mutant populations contain 2(n) (n=0, 1, 2.) origins of replication...
  5. Polidoro M, De Biase D, Montagnini B, Guarrera L, Cavallo S, Valenti P, et al. The expression of the dodecameric ferritin in Listeria spp. is induced by iron limitation and stationary growth phase. Gene. 2002;296:121-8 pubmed
  6. Kalinin A, Rak A, Shcherbakov D, Bayer P. 1H, 13C and 15N resonance assignments of the ribosome-associated cold shock response protein Yfia of Escherichia coli. J Biomol NMR. 2002;23:335-6 pubmed
  7. Clarke D, Coulson J, Baillie R, Campopiano D. Biotinylation in the hyperthermophile Aquifex aeolicus. Eur J Biochem. 2003;270:1277-87 pubmed
  8. Anderson T, Sauer R. Role of an N(cap) residue in determining the stability and operator-binding affinity of Arc repressor. Biophys Chem. 2003;100:341-50 pubmed
    ..Based upon the results presented here, revised sequence alignments of the MetJ and NikR subfamilies with Arc and other ribbon-helix-helix proteins are proposed. ..
  9. Camara J, Skarstad K, Crooke E. Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein. J Bacteriol. 2003;185:3244-8 pubmed
    ..Analysis of deltahda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability...
  10. Chang C, Pl ckthun A, Wlodawer A. Crystal structure of a truncated version of the phage lambda protein gpD. Proteins. 2004;57:866-8 pubmed publisher
  11. Johnson J, Clabots C, Rosen H. Effect of inactivation of the global oxidative stress regulator oxyR on the colonization ability of Escherichia coli O1:K1:H7 in a mouse model of ascending urinary tract infection. Infect Immun. 2006;74:461-8 pubmed publisher
    ..coli. They also suggest that neutrophil phagocyte oxidase is not critical for defense against E. coli UTI and that the major oxidative stresses against which OxyR protects E. coli within the host milieu are not phagocyte derived...
  12. Turner A, Beavis J, Stephens J, Greenwood J, Gewert C, Thomas N, et al. Construction and phase I clinical evaluation of the safety and immunogenicity of a candidate enterotoxigenic Escherichia coli vaccine strain expressing colonization factor antigen CFA/I. Infect Immun. 2006;74:1062-71 pubmed publisher
    ..This live attenuated bacterial vaccine is safe and immunogenic in healthy adult volunteers...
  13. Kitagawa R, Takaya A, Yamamoto T. Dual regulatory pathways of flagellar gene expression by ClpXP protease in enterohaemorrhagic Escherichia coli. Microbiology. 2011;157:3094-103 pubmed publisher
    ..We have also shown that the degradation of FlhD and FlhC by ClpXP is responsible for downregulating flagellar expression even when LEE expression is induced...
  14. Springs S, Bass S, Bowman G, Nodelman I, Schutt C, McLendon G. A multigeneration analysis of cytochrome b(562) redox variants: evolutionary strategies for modulating redox potential revealed using a library approach. Biochemistry. 2002;41:4321-8 pubmed
  15. Miller A, Wang L, Kendall D. SecB modulates the nucleotide-bound state of SecA and stimulates ATPase activity. Biochemistry. 2002;41:5325-32 pubmed
    ..The inhibition of SecA ATPase activity by sodium azide in the presence of IMVs and a functional signal peptide further indicates that SecB promotes the ATP-bound form of SecA...
  16. Jiang Z, Mu Y, Li W, Yan G, Luo G. The progress in mechanism of selenoprotein biosynthesis. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002;34:395-9 pubmed
    ..The researchers have made exploration in selenoprotein engineering by virtue of the mechanism of selenocysteine incorporation in Escherichia coli. ..
  17. Chivers P, Sauer R. NikR repressor: high-affinity nickel binding to the C-terminal domain regulates binding to operator DNA. Chem Biol. 2002;9:1141-8 pubmed
    ..Nickel binding to a set of low-affinity NikR sites resulted in an additional large increase in operator affinity and substantially increased the size of the NikR footprint on the operator...
  18. Etienne F, Spector D, Brot N, Weissbach H. A methionine sulfoxide reductase in Escherichia coli that reduces the R enantiomer of methionine sulfoxide. Biochem Biophys Res Commun. 2003;300:378-82 pubmed
    ..A new member of the Msr family (fRMsr) has been identified in E. coli extracts that reduces free met-R-(o) to met. Partial purification of FRMsr has been obtained using extracts from an MsrA/MsrB double mutant of E. coli...
  19. Walsh N, Alba B, Bose B, Gross C, Sauer R. OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain. Cell. 2003;113:61-71 pubmed
    ..Peptide binding to inhibitory PDZ domains in proteases related to DegS, including DegP/HtrA, may also regulate the degradation of specific substrates by these enzymes...
  20. Gong S, Richard H, Foster J. YjdE (AdiC) is the arginine:agmatine antiporter essential for arginine-dependent acid resistance in Escherichia coli. J Bacteriol. 2003;185:4402-9 pubmed
    ..The data also illustrate that the AdiA decarboxylase:AdiC antiporter system is designed to function only at acid levels sufficient to harm the cell...
  21. Boylan J, Posey J, Gherardini F. Borrelia oxidative stress response regulator, BosR: a distinctive Zn-dependent transcriptional activator. Proc Natl Acad Sci U S A. 2003;100:11684-9 pubmed publisher
    ..On the basis of these data, we propose that, despite the similarity to PerR, BosR functions primarily as a transcriptional activator, not a repressor of oxidative stress response, in B. burgdorferi...
  22. Petersen A, Christensen J, Kuhnert P, Bisgaard M, Olsen J. Vertical transmission of a fluoroquinolone-resistant Escherichia coli within an integrated broiler operation. Vet Microbiol. 2006;116:120-8 pubmed publisher
  23. Paudyal S, Alfonso Prieto M, Carnevale V, Redhu S, Klein M, Nicholson A. Combined computational and experimental analysis of a complex of ribonuclease III and the regulatory macrodomain protein, YmdB. Proteins. 2015;83:459-72 pubmed publisher
    ..The features of the modeled complex relevant to potential RNase III regulatory mechanisms are discussed. ..
  24. Yee A, Chang X, Pineda Lucena A, Wu B, Semesi A, Le B, et al. An NMR approach to structural proteomics. Proc Natl Acad Sci U S A. 2002;99:1825-30 pubmed publisher
    ..Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins...
  25. Zucchini C, Strippoli P, Biolchi A, Solmi R, Lenzi L, D Addabbo P, et al. The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1. Int J Mol Med. 2003;11:697-704 pubmed
    ..Study of TRUB1 (and TRUB2) should facilitate understanding of the molecular mechanisms of RNA modification and the involvement of psi synthases in human pathology, including dyskeratosis-like diseases. ..
  26. Noble M, Zeelen J, Wierenga R, Mainfroid V, Goraj K, Gohimont A, et al. Structure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution. Acta Crystallogr D Biol Crystallogr. 1993;49:403-17 pubmed
    ..In addition, TIM from E. coli shows peculiarities in its dimer interface, and in the packing of core residues within the beta-barrel. ..
  27. Carr P, Cheah E, Suffolk P, Vasudevan S, Dixon N, Ollis D. X-ray structure of the signal transduction protein from Escherichia coli at 1.9 A. Acta Crystallogr D Biol Crystallogr. 1996;52:93-104 pubmed
    ..The effects of crystal packing forces are discussed and potential interaction sites with other proteins and effector molecules are identified. ..
  28. Rumpel S, Kim H, Vijayan V, Becker S, Zweckstetter M. Backbone resonance assignment of the homodimeric, 35 kDa chaperone CesT from enteropathogenic Escherichia coli. J Biomol NMR. 2005;31:377-8 pubmed
  29. Mellies J, Barron A, Haack K, Korson A, Oldridge D. The global regulator Ler is necessary for enteropathogenic Escherichia coli colonization of Caenorhabditis elegans. Infect Immun. 2006;74:64-72 pubmed publisher
  30. Nakanishi N, Abe H, Ogura Y, Hayashi T, Tashiro K, Kuhara S, et al. ppGpp with DksA controls gene expression in the locus of enterocyte effacement (LEE) pathogenicity island of enterohaemorrhagic Escherichia coli through activation of two virulence regulatory genes. Mol Microbiol. 2006;61:194-205 pubmed publisher
    ..These findings suggest that the regulation of virulence genes in EHEC is integrated with E. coli's stringent response system, through the regulation of virulence regulatory genes...
  31. Hall D, Bond C, Leonard G, Watt C, Berry A, Hunter W. Structure of tagatose-1,6-bisphosphate aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases. J Biol Chem. 2002;277:22018-24 pubmed publisher
    ..The availability of more space and a wider range of aldehyde partners used by TBPA together with the highly specific nature of FBPA suggest that TBPA might be a preferred enzyme to modify for use in biotransformation chemistry...
  32. Young J, Hartl F. A stress sensor for the bacterial periplasm. Cell. 2003;113:1-2 pubmed
    ..This interaction relieves the inhibition of the neighboring protease domain of DegS, triggering a proteolysis cascade that leads to the sigma(E)-driven expression of periplasmic chaperones...
  33. Andrews S, Robinson A, Rodr guez Qui ones F. Bacterial iron homeostasis. FEMS Microbiol Rev. 2003;27:215-37 pubmed
  34. March s O, Ledger T, Boury M, Ohara M, Tu X, Goffaux F, et al. Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G2/M transition. Mol Microbiol. 2003;50:1553-67 pubmed
    ..These cells arrested at the G2/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle...
  35. Nakano H, Uchiyama S, Yoshida T, Ohkubo T, Kato H, Yamagata Y, et al. Crystallization and preliminary X-ray crystallographic studies of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly. Acta Crystallogr D Biol Crystallogr. 2002;58:124-6 pubmed
    ..The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 46.02, b = 49.27, c = 49.37 A, beta = 110.1 degrees. The currently refined structure indicates that RRF has a tRNA-like L-shaped conformation...
  36. Tajkhorshid E, Nollert P, Jensen M, Miercke L, O Connell J, Stroud R, et al. Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science. 2002;296:525-30 pubmed publisher
    ..Both simulations and observations revealed a more regular distribution of channel water and an increased water permeability for the W48F/F200T mutant...
  37. Zhao G, Ceci P, Ilari A, Giangiacomo L, Laue T, Chiancone E, et al. Iron and hydrogen peroxide detoxification properties of DNA-binding protein from starved cells. A ferritin-like DNA-binding protein of Escherichia coli. J Biol Chem. 2002;277:27689-96 pubmed publisher
    ..In the latter process a hydrous ferric oxide mineral core is produced within the protein, thus avoiding oxidative damage mediated by Fenton chemistry...
  38. Haebel P, Goldstone D, Katzen F, Beckwith J, Metcalf P. The disulfide bond isomerase DsbC is activated by an immunoglobulin-fold thiol oxidoreductase: crystal structure of the DsbC-DsbDalpha complex. EMBO J. 2002;21:4774-84 pubmed
    ..Our results explain how DsbC is selectively activated by DsbD using electrons derived from the cytoplasm...
  39. Naas T, Aubert D, Fortineau N, Nordmann P. Cloning and sequencing of the beta-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus. FEMS Microbiol Lett. 2002;215:81-7 pubmed
    ..This work provides further evidence of the molecular heterogeneity of class C beta-lactamases. ..
  40. Hu Z, Saez C, Lutkenhaus J. Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE. J Bacteriol. 2003;185:196-203 pubmed
    ..These results suggest that MinE induces a conformational change in MinD bound to the bilayer that results in the release of MinC. Also, it is argued that binding of MinD to the membrane activates MinC...
  41. Altboum Z, Levine M, Galen J, Barry E. Genetic characterization and immunogenicity of coli surface antigen 4 from enterotoxigenic Escherichia coli when it is expressed in a Shigella live-vector strain. Infect Immun. 2003;71:1352-60 pubmed
    ..These findings pave the way for CS4 to be included in multivalent ETEC vaccines, including an attenuated Shigella live-vector-based ETEC vaccine...
  42. Kurihara T, Mihara H, Kato S, Yoshimura T, Esaki N. Assembly of iron-sulfur clusters mediated by cysteine desulfurases, IscS, CsdB and CSD, from Escherichia coli. Biochim Biophys Acta. 2003;1647:303-9 pubmed
  43. Hoff K, Cupp Vickery J, Vickery L. Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system. J Biol Chem. 2003;278:37582-9 pubmed publisher
    ..The results are discussed in the context of a structural model based on the crystallographic structure of the DnaK peptide-binding domain...
  44. McCool J, Ford C, Sandler S. A dnaT mutant with phenotypes similar to those of a priA2::kan mutant in Escherichia coli K-12. Genetics. 2004;167:569-78 pubmed publisher
    ..coli K-12. Instead, this mutant dnaT gene was found to complement the E. coli K-12 dnaT822 mutant phenotypes. The significance of these results is discussed in terms of models for replication restart...
  45. Ludwig A, von Rhein C, Bauer S, H ttinger C, Goebel W. Molecular analysis of cytolysin A (ClyA) in pathogenic Escherichia coli strains. J Bacteriol. 2004;186:5311-20 pubmed publisher
    ..The presented data indicate that cytolysin A can play a role only for some of the pathogenic E. coli strains...
  46. Brown P, Beckett D. Use of binding enthalpy to drive an allosteric transition. Biochemistry. 2005;44:3112-21 pubmed publisher
    ..This transition is "paid for" by the enthalpy that would have otherwise been realized from the formation of noncovalent bonds between the ligand and repressor monomer...
  47. Wang H, Li Q, Fang Y, Yu S, Tang B, Na L, et al. Biochemical and functional characterization of the periplasmic domain of the outer membrane protein A from enterohemorrhagic Escherichia coli. Microbiol Res. 2016;182:109-15 pubmed publisher
    ..In addition, OmpAper contributes to the adhesion of bacteria to HeLa cells in vitro and ex vivo. These results provide an additional understanding of the role of OmpA in EHEC physiology and pathogenesis. ..
  48. Sperandio V, Torres A, Kaper J. Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol. 2002;43:809-21 pubmed
  49. Zeth K, Ravelli R, Paal K, Cusack S, Bukau B, Dougan D. Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA. Nat Struct Biol. 2002;9:906-11 pubmed publisher
    ..From this model, we proposed a molecular mechanism to explain the ClpS-mediated switch in ClpA substrate specificity...
  50. Ueno H, Yonesaki T. Role of Escherichia coli Hfq in late-gene silencing of bacteriophage T4 dmd mutant. Genes Genet Syst. 2002;77:301-8 pubmed
    ..We also showed that purified Hfq protein bound stoichiometrically to soc RNA. These results strongly suggest that the hfq gene has a stimulatory role in dmd mutant-specific mRNA degradation...
  51. Lutkenhaus J. Dynamic proteins in bacteria. Curr Opin Microbiol. 2002;5:548-52 pubmed
    ..These studies further demonstrate that the eukaryotic cytoskeleton had its origins in bacteria...
  52. Van Lanen S, Kinzie S, Matthieu S, Link T, Culp J, Iwata Reuyl D. tRNA modification by S-adenosylmethionine:tRNA ribosyltransferase-isomerase. Assay development and characterization of the recombinant enzyme. J Biol Chem. 2003;278:10491-9 pubmed publisher
    ..7 microm and k(cat) = 14.7 min(-1)), demonstrating that the anticodon stem-loop is sufficient for recognition and catalysis by QueA...
  53. Gille C, Goede A, Schl etelburg C, Preissner R, Kloetzel P, G bel U, et al. A comprehensive view on proteasomal sequences: implications for the evolution of the proteasome. J Mol Biol. 2003;326:1437-48 pubmed
    ..The structure-based multiple proteasomal alignment of 433 sequences from 143 organisms can be downloaded from the URL dagger and will be updated regularly...
  54. Kim P, Banack T, Lerman D, Tracy J, Camara J, Crooke E, et al. Identification of a novel membrane-associated gene product that suppresses toxicity of a TrfA peptide from plasmid RK2 and its relationship to the DnaA host initiation protein. J Bacteriol. 2003;185:1817-24 pubmed
    ..Recently, the Dp was independently shown to help prevent overinitiation in E. coli and was termed Hda (S. Kato and T. Katayama, EMBO J. 20:4253-4262, 2001)...
  55. Echave P, Tamarit J, Cabiscol E, Ros J. Novel antioxidant role of alcohol dehydrogenase E from Escherichia coli. J Biol Chem. 2003;278:30193-8 pubmed publisher
    ..We therefore propose that AdhE acts as a H2O2 scavenger in Escherichia coli cells grown under aerobic conditions...
  56. Ma Z, Richard H, Foster J. pH-Dependent modulation of cyclic AMP levels and GadW-dependent repression of RpoS affect synthesis of the GadX regulator and Escherichia coli acid resistance. J Bacteriol. 2003;185:6852-9 pubmed
    ..The presence of multiple pH control pathways governing expression of this acid resistance system is thought to reflect different environmental routes to a low pH...
  57. Burnouf D, Olieric V, Wagner J, Fujii S, Reinbolt J, Fuchs R, et al. Structural and biochemical analysis of sliding clamp/ligand interactions suggest a competition between replicative and translesion DNA polymerases. J Mol Biol. 2004;335:1187-97 pubmed
  58. Ackerley D, Gonzalez C, Park C, Blake R, Keyhan M, Matin A. Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol. 2004;70:873-82 pubmed
    ..The results nevertheless suggest that YieF may be an even more suitable candidate for further studies than ChrR...
  59. Severinova E, Severinov K. Localization of the Escherichia coli RNA polymerase beta' subunit residue phosphorylated by bacteriophage T7 kinase Gp0.7. J Bacteriol. 2006;188:3470-6 pubmed publisher
  60. Vazquez Juarez R, Kuriakose J, Rasko D, Ritchie J, Kendall M, Slater T, et al. CadA negatively regulates Escherichia coli O157:H7 adherence and intestinal colonization. Infect Immun. 2008;76:5072-81 pubmed publisher
    ..In the infant rabbit model, the cadA mutant outcompeted the wild-type strain in the ileum but not in the cecum or mid-colon, raising the possibility that CadA negatively regulates EHEC pathogenicity in a tissue-specific fashion...
  61. Rudi o Pi era E, Morales Arrieta S, Rojas Trejo S, Horjales E. Structural flexibility, an essential component of the allosteric activation in Escherichia coli glucosamine-6-phosphate deaminase. Acta Crystallogr D Biol Crystallogr. 2002;58:10-20 pubmed
    ..The concerted character of the allosteric transition is also analysed in view of the apparent dynamics of the conformers...
  62. Richard S, Ferrer J, Bowman M, Lillo A, Tetzlaff C, Cane D, et al. Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway. J Biol Chem. 2002;277:8667-72 pubmed publisher
  63. Iverson T, Luna Chavez C, Croal L, Cecchini G, Rees D. Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. J Biol Chem. 2002;277:16124-30 pubmed publisher
    ..This acidic residue, Glu-C29, in the E. coli enzyme may act as a proton shuttle from the quinol during enzyme turnover...
  64. Kato S, Mihara H, Kurihara T, Takahashi Y, Tokumoto U, Yoshimura T, et al. Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: implications for the mechanism of iron-sulfur cluster assembly. Proc Natl Acad Sci U S A. 2002;99:5948-52 pubmed publisher
  65. Urbonavicius J, Durand J, Bj rk G. Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri. J Bacteriol. 2002;184:5348-57 pubmed
    ..Moreover, lack of Psi55 in tRNA of human pathogen Shigella flexneri leads to a reduced expression of several virulence-associated genes...
  66. Hamabata T, Tanaka T, Ozawa A, Shima T, Sato T, Takeda Y. Genetic variation in the flanking regions of Shiga toxin 2 gene in Shiga toxin-producing Escherichia coli O157:H7 isolated in Japan. FEMS Microbiol Lett. 2002;215:229-36 pubmed
    ..These results suggest that the DNA sequences surrounding the stx(2) genes are preferably integrated by IS1203v in wild-type Shiga toxin-producing E. coli strains. ..
  67. Lanz R, Kuhnert P, Boerlin P. Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet Microbiol. 2003;91:73-84 pubmed
    ..coli isolates from animals...
  68. Uehara T, Park J. Identification of MpaA, an amidase in Escherichia coli that hydrolyzes the gamma-D-glutamyl-meso-diaminopimelate bond in murein peptides. J Bacteriol. 2003;185:679-82 pubmed
  69. Jordan S, Cronan J. The Escherichia coli lipB gene encodes lipoyl (octanoyl)-acyl carrier protein:protein transferase. J Bacteriol. 2003;185:1582-9 pubmed
    ..A nonhydrolyzable lipoyl-AMP analogue was found to competitively inhibit both ACP-dependent and ATP-dependent reactions of LplA, suggesting that the same active site catalyzes two chemically diverse reactions. ..
  70. Spector D, Etienne F, Brot N, Weissbach H. New membrane-associated and soluble peptide methionine sulfoxide reductases in Escherichia coli. Biochem Biophys Res Commun. 2003;302:284-9 pubmed
    ..coli. In addition, a new soluble Msr activity was also detected in the soluble extracts of the double mutant that specifically reduces the S epimer of met(o) in proteins. ..
  71. Howard M, Rutenberg A. Pattern formation inside bacteria: fluctuations due to the low copy number of proteins. Phys Rev Lett. 2003;90:128102 pubmed publisher
    ..We find that, for some parameter regions, the protein concentrations are low enough that fluctuations are essential for the generation of patterns. We also examine the role of fluctuations in constraining protein concentration levels...
  72. Mass E, Majdalani N, Gottesman S. Regulatory roles for small RNAs in bacteria. Curr Opin Microbiol. 2003;6:120-4 pubmed
    ..Small RNAs can both positively and negatively regulate translation, can simultaneously regulate multiple mRNA targets, and can change the pattern of polarity within an operon...
  73. Sand O, Gingras M, Beck N, Hall C, Trun N. Phenotypic characterization of overexpression or deletion of the Escherichia coli crcA, cspE and crcB genes. Microbiology. 2003;149:2107-17 pubmed publisher
    ..7-fold activation of rcsA. These activities were augmented when crcB was overexpressed with cspE (100-fold camphor resistance and 2.1-fold induction of rcsA)...
  74. Ma Z, Gong S, Richard H, Tucker D, Conway T, Foster J. GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12. Mol Microbiol. 2003;49:1309-20 pubmed
    ..The number of regulatory proteins (five), sigma factors (two) and regulatory feedback loops focused on gadA/BC expression make this one of the most intensively regulated systems in E. coli...
  75. Chen J, Lu G, Lin J, Davidson A, Quiocho F. A tweezers-like motion of the ATP-binding cassette dimer in an ABC transport cycle. Mol Cell. 2003;12:651-61 pubmed
    ..The two nucleotide binding domains of the dimer open and close like a pair of tweezers, suggesting a regulatory mechanism for ATPase activity that may be tightly coupled to translocation...
  76. D Souza J, Samuel G, Reeves P. Evolutionary origins and sequence of the Escherichia coli O4 O-antigen gene cluster. FEMS Microbiol Lett. 2005;244:27-32 pubmed publisher
    ..coli O26; indicating a common ancestor. These lateral transfer events observed within O-antigen gene clusters may occur as part of the evolution of the pathogenic clones...
  77. Rangarajan E, Li Y, Iannuzzi P, Tocilj A, Hung L, Matte A, et al. Crystal structure of a dodecameric FMN-dependent UbiX-like decarboxylase (Pad1) from Escherichia coli O157: H7. Protein Sci. 2004;13:3006-16 pubmed publisher
    ..This exposure affects the location of the substrate binding site and, specifically, its access to the FMN cofactor. Therefore, Pad1 forms a separate family, distinguishable from the HFCD family...
  78. Meinhardt H, de Boer P. Pattern formation in Escherichia coli: a model for the pole-to-pole oscillations of Min proteins and the localization of the division site. Proc Natl Acad Sci U S A. 2001;98:14202-7 pubmed publisher
    ..On time average, MinC concentration is highest at the poles, forcing FtsZ assembly to the center. The mechanism is self-organizing and does not require any other hypothetical topological determinant...
  79. Merlin C, McAteer S, Masters M. Tools for characterization of Escherichia coli genes of unknown function. J Bacteriol. 2002;184:4573-81 pubmed
    ..Replacements of yacF, yacG, yacH, yacK (cueO), yacL, ruvA, ruvB, yabB, and yabC made with the cassette were used to verify its properties...
  80. Michel G, Sauv V, Larocque R, Li Y, Matte A, Cygler M. The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot. Structure. 2002;10:1303-15 pubmed
    ..The conserved residues in this novel family of 2'O-methyltransferases cluster in the knotted region, suggesting the location of the catalytic and AdoMet binding sites...
  81. Tucker D, Tucker N, Conway T. Gene expression profiling of the pH response in Escherichia coli. J Bacteriol. 2002;184:6551-8 pubmed
    ..Thus, the acid-inducible genes clustered in the gadA region appear to be involved in glutatmate-dependent acid resistance, although their specific roles remain to be elucidated...
  82. Yang W. Damage repair DNA polymerases Y. Curr Opin Struct Biol. 2003;13:23-30 pubmed
  83. Mileykovskaya E, Fishov I, Fu X, Corbin B, Margolin W, Dowhan W. Effects of phospholipid composition on MinD-membrane interactions in vitro and in vivo. J Biol Chem. 2003;278:22193-8 pubmed publisher
    ..These results suggest that MinD has a preference for anionic phospholipids and that MinD oscillation behavior, and therefore cell division site selection, may be regulated by membrane phospholipid composition...
  84. Ueta M, Yoshida H, Wada C, Baba T, Mori H, Wada A. Ribosome binding proteins YhbH and YfiA have opposite functions during 100S formation in the stationary phase of Escherichia coli. Genes Cells. 2005;10:1103-12 pubmed publisher
    ..Since 100S particles were seen in the yfiA deletion mutant containing RMF and YhbH, YhbH probably converts immature 90S ribosomes into mature 100S particles...
  85. Bielaszewska M, Prager R, Zhang W, Friedrich A, Mellmann A, Tsch pe H, et al. Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM. Appl Environ Microbiol. 2006;72:1900-9 pubmed publisher
    ..The variability of PFGE patterns for the progeny of a single colony must be considered when interpreting PFGE patterns in SF EHEC O157-associated outbreaks...
  86. Jensen S, Reeves P. Molecular evolution of the GDP-mannose pathway genes (manB and manC) in Salmonella enterica. Microbiology. 2001;147:599-610 pubmed publisher
    ..enterica and more closely related species...
  87. BOHM A, Diez J, Diederichs K, Welte W, Boos W. Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli: implications for mal gene regulation, inducer exclusion, and subunit assembly. J Biol Chem. 2002;277:3708-17 pubmed
    ..The validity of the modeled MalK structure was verified by structure-directed mutagenesis of amino acids located within the proposed MalK-MalT interaction site...
  88. Ramirez Arcos S, Salimnia H, Bergevin I, Paradis M, Dillon J. Expression of Neisseria gonorrhoeae cell division genes ftsZ, ftsE and minD is influenced by environmental conditions. Res Microbiol. 2001;152:781-91 pubmed
    ..These data infer that gonococci regulate their cell division in response to different environments...
  89. Subbarayan P, Deutscher M. Escherichia coli RNase M is a multiply altered form of RNase I. RNA. 2001;7:1702-7 pubmed
    ..We also suggest that RNase M is just one of several previously described endoribonuclease activities that are actually manifestations of RNase I...
  90. Gustavsson N, Diez A, Nystr m T. The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage. Mol Microbiol. 2002;43:107-17 pubmed
    ..Moreover, combination of usp mutations had no additive effect on UV sensitivity indicating that they are all co-operating and required in the same pathway, which could explain the co-ordinated regulation of the genes...
  91. Arjunan P, Nemeria N, Brunskill A, Chandrasekhar K, Sax M, Yan Y, et al. Structure of the pyruvate dehydrogenase multienzyme complex E1 component from Escherichia coli at 1.85 A resolution. Biochemistry. 2002;41:5213-21 pubmed
    ..It also provides the first representative example for the entire family of homodimeric (alpha2) E1 multienzyme complex components, and should serve as a model for this class of enzymes...
  92. Pedersen K, Christensen S, Gerdes K. Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins. Mol Microbiol. 2002;45:501-10 pubmed
    ..These results preclude that RelE and ChpAK mediate cell killing during the conditions used here. In vivo and in vitro analyses of a mutant RelE protein supported that inhibition of colony formation was due to inhibition of translation...