Escherichia coli

Summary

Alias: Escherichia coli retron Ec86, Escherichia coli retron Ec79, Escherichia coli retron Ec67, Escherichia coli retron Ec107, Escherichia coli (Migula 1895) Castellani and Chalmers 1919, "Bacillus coli" Migula 1895, "Bacterium coli" (Migula 1895) Lehmann and Neumann 1896, Bacterium coli commune, Bacterium coli, Bacillus coli, "Bacterium coli commune" Escherich 1885, Eschericia coli, Escherchia coli, Enterococcus coli, bacterium 10a, bacterium E3

Top Publications

  1. ncbi Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
  2. ncbi The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 145:1005-14. 1999
  3. ncbi Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons
    C Levesque
    Departement de Biochimie, Faculte des sciences et de genie, Universite Laval, Ste Foy, Quebec, Canada
    Gene 142:49-54. 1994
  4. pmc Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain
    Charles M Dozois
    Department of Biology, Washington University, Campus Box 1137, One Brookings Drive, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 100:247-52. 2003
  5. ncbi Gene cassettes: a new class of mobile element
    G D Recchia
    CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia
    Microbiology 141:3015-27. 1995
  6. ncbi A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide
    Mourad Sabri
    Institut National de la Recherche Scientifique, INRS Institut Armand Frappier, 531 Boul des Prairies, Laval, Quebec, Canada H7V 1B7
    Microbiology 152:745-58. 2006
  7. ncbi Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21
    L Sundstrom
    Department of Pharmaceutical Microbiology, Uppsala University, Sweden
    Mol Gen Genet 213:191-201. 1988
  8. pmc Genomic analysis of a pathogenicity island in uropathogenic Escherichia coli CFT073: distribution of homologous sequences among isolates from patients with pyelonephritis, cystitis, and Catheter-associated bacteriuria and from fecal samples
    D M Guyer
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 66:4411-7. 1998
  9. pmc Pathogenicity island sequences of pyelonephritogenic Escherichia coli CFT073 are associated with virulent uropathogenic strains
    J S Kao
    Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA
    Infect Immun 65:2812-20. 1997
  10. pmc Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain
    D L Provence
    Department of Biology, Washington University, St Louis, Missouri 63130
    Infect Immun 62:1369-80. 1994

Detail Information

Publications216 found, 100 shown here

  1. ncbi Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
    Almost all clinical enterohaemorrhagic Escherichia coli (EHEC) O157:H7 isolates harbour a large virulence plasmid designated pO157. In this study, pO157 of EHEC O157:H7 reference strain EDL 933 was characterized at the molecular level...
  2. ncbi The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 145:1005-14. 1999
    Shiga-toxin-producing Escherichia coli (STEC) of different serotypes are known to harbour large plasmids...
  3. ncbi Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons
    C Levesque
    Departement de Biochimie, Faculte des sciences et de genie, Universite Laval, Ste Foy, Quebec, Canada
    Gene 142:49-54. 1994
    ..The strongest promoter is the version (TTGACAN17TAAACT) found in plasmid R388 and in transposon Tn1696. This promoter is six times more efficient than the derepressed tac promoter...
  4. pmc Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain
    Charles M Dozois
    Department of Biology, Washington University, Campus Box 1137, One Brookings Drive, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 100:247-52. 2003
    b>Escherichia coli is a diverse bacterial species that comprises commensal nonpathogenic strains such as E. coli K-12 and pathogenic strains that cause a variety of diseases in different host species. Avian pathogenic E...
  5. ncbi Gene cassettes: a new class of mobile element
    G D Recchia
    CSIRO Division of Biomolecular Engineering, Sydney Laboratory, North Ryde, NSW, Australia
    Microbiology 141:3015-27. 1995
  6. ncbi A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide
    Mourad Sabri
    Institut National de la Recherche Scientifique, INRS Institut Armand Frappier, 531 Boul des Prairies, Laval, Quebec, Canada H7V 1B7
    Microbiology 152:745-58. 2006
    ..ion transporters, homologous to Salmonella enterica SitABCD, has been identified in the avian pathogenic Escherichia coli (APEC) strain chi7122...
  7. ncbi Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21
    L Sundstrom
    Department of Pharmaceutical Microbiology, Uppsala University, Sweden
    Mol Gen Genet 213:191-201. 1988
    ..Similar structures were found at the 3' ends of the antibiotic resistance genes in Tn7, which could indicate similar recombination mechanisms to be effective in the evolutionary construction of all these different resistance elements...
  8. pmc Genomic analysis of a pathogenicity island in uropathogenic Escherichia coli CFT073: distribution of homologous sequences among isolates from patients with pyelonephritis, cystitis, and Catheter-associated bacteriuria and from fecal samples
    D M Guyer
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 66:4411-7. 1998
    Urinary tract infection is the most frequently diagnosed kidney and urologic disease and Escherichia coli is by far the most common etiologic agent...
  9. pmc Pathogenicity island sequences of pyelonephritogenic Escherichia coli CFT073 are associated with virulent uropathogenic strains
    J S Kao
    Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA
    Infect Immun 65:2812-20. 1997
    Urinary tract infection is the most frequently diagnosed kidney and urologic disease, and Escherichia coli is by far the most common etiologic agent...
  10. pmc Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain
    D L Provence
    Department of Biology, Washington University, St Louis, Missouri 63130
    Infect Immun 62:1369-80. 1994
    ..the isolation and characterization of a gene that may be important in the adherence of avian pathogenic Escherichia coli to the avian respiratory tract. The E...
  11. ncbi The newly characterized colicin Y provides evidence of positive selection in pore-former colicin diversification
    M A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA
    Microbiology 146:1671-7. 2000
    ..have been proposed in the process of protein diversification of the large family of antimicrobial toxins of Escherichia coli, known as the colicins...
  12. pmc Genetic structure and distribution of four pathogenicity islands (PAI I(536) to PAI IV(536)) of uropathogenic Escherichia coli strain 536
    Ulrich Dobrindt
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, D 97070 Wurzburg, Germany
    Infect Immun 70:6365-72. 2002
    For the uropathogenic Escherichia coli strain 536 (O6:K15:H31), the DNA sequences of three pathogenicity islands (PAIs) (PAI I(536) to PAI III(536)) and their flanking regions (about 270 kb) were determined to further characterize the ..
  13. pmc Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K
    Matija Rijavec
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana, Slovenia
    Appl Environ Microbiol 73:1029-32. 2007
    Colicin K exhibited pronounced inhibitory activity against uropathogenic Escherichia coli (UPEC) strains...
  14. ncbi Risk factors for and outcomes of bloodstream infection caused by extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella species in children
    Theoklis E Zaoutis
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    Pediatrics 115:942-9. 2005
    The increasing prevalence of infections caused by extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella species (ESBL-EK) has become a growing concern in the hospitalized patient population...
  15. ncbi 99mTc-labeled antimicrobial peptide ubiquicidin (29-41) accumulates less in Escherichia coli infection than in Staphlococcus aureus infection
    Muhammad Saeed Akhtar
    Punjab Institute of Nuclear Medicine, Faisalabad, Pakistan
    J Nucl Med 45:849-56. 2004
    99mTc-Labeled antimicrobial peptide ubiquicidin, (99m)Tc-UBI (29-41) in a freeze-dried kit, was evaluated as a bacterial infection-seeking agent in Staphlococcus aureus- and Escherichia coli-induced infections.
  16. doi Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomics
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    Anal Chem 82:2717-25. 2010
    Six protein biomarkers from two strains of Escherichia coli O157:H7 and one non-O157:H7, nonpathogenic strain of E...
  17. ncbi Expression in Escherichia coli of Y5 mutant and N-terminal domain-deleted DNA gyrase B proteins affects strongly plasmid maintenance
    L Brino
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France
    Plasmid 39:21-34. 1998
    b>Escherichia coli DNA gyrase B subunit (GyrB) is composed of a 43-kDa N-terminal domain containing an ATP-binding site and a 47-kDa C-terminal domain involved in the interaction with the gyrase A subunit (GyrA)...
  18. ncbi Expression in Escherichia coli of Y5-mutant and N-terminal domain-deleted DNA gyrase B proteins affects strongly plasmid maintenance
    L Brino
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, ULP, Illkirch, France
    Plasmid 38:188-201. 1997
    b>Escherichia coli DNA gyrase B subunit (GyrB) is composed of a 43-kDa N-terminal domain containing an ATP-binding site and a 47-kDa C-terminal domain involved in the interaction with the gyrase A subunit (GyrA)...
  19. ncbi Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera toxin
    T K Sixma
    BIOSON Research Institute, University of Groningen, The Netherlands
    J Mol Biol 230:890-918. 1993
    Heat-labile enterotoxin (LT) from Escherichia coli is a bacterial protein toxin with an AB5 multimer structure, in which the B pentamer has a membrane binding function and the A subunit is needed for enzymatic activity...
  20. pmc Genetic interactions between the Escherichia coli umuDC gene products and the beta processivity clamp of the replicative DNA polymerase
    M D Sutton
    Biology Department, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 183:2897-909. 2001
    The Escherichia coli umuDC gene products encode DNA polymerase V, which participates in both translesion DNA synthesis (TLS) and a DNA damage checkpoint control...
  21. ncbi Linkage between fructose 1,6-bisphosphate binding and the dimer-tetramer equilibrium of Escherichia coli glycerol kinase: critical behavior arising from change of ligand stoichiometry
    Peng Yu
    Department of Biochemistry and Biophysics, Center for Advanced Biomolecular Research, Texas A and M University, College Station, Texas 77843 2128, USA
    Biochemistry 42:4243-52. 2003
    b>Escherichia coli glycerol kinase (EC 2.7.1...
  22. pmc The Escherichia coli BarA-UvrY two-component system is a virulence determinant in the urinary tract
    Henrik Tomenius
    Swedish Institute for Infectious Disease Control, SE 17182 Solna, Sweden
    BMC Microbiol 6:27. 2006
    ..and the Pseudomonas spp GacS-GacA all belong to the same orthologous family of two-component systems as the Escherichia coli BarA-UvrY...
  23. ncbi An activator of glutamate decarboxylase genes regulates the expression of enteropathogenic Escherichia coli virulence genes through control of the plasmid-encoded regulator, Per
    S Shin
    Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Mol Microbiol 41:1133-50. 2001
    Enteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhoea in a number of developing countries and is the prototype of pathogenic bacteria that cause attaching and effacing (A/E) intestinal lesions...
  24. ncbi Effects of heat stress on the antimicrobial drug resistance of Escherichia coli of the intestinal flora of swine
    M H Moro
    Department of Microbiology, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    J Appl Microbiol 88:836-44. 2000
    The effects of heat stress on the antimicrobial drug resistance of Escherichia coli of the intestinal tract of swine were studied in animals from a farm that had not been supplementing antimicrobials in feed for the past 10 years...
  25. doi New enterovirulent Escherichia coli serogroup 64474 showing antigenic and genotypic relationships to Shigella boydii 16
    Armando Navarro
    Departamento de Salud Publica, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Mexico City DF 04510, Mexico
    J Med Microbiol 59:453-61. 2010
    Studies based on the analysis of housekeeping genes indicate that Escherichia coli and all Shigella species, except for Shigella boydii type 13, belong to a single species...
  26. ncbi Valyl-tRNA synthetase from Escherichia coli MALDI-MS identification of the binding sites for L-valine or for noncognate amino acids upon qualitative comparative labeling with reactive amino-acid analogs
    C Hountondji
    Laboratoire de Biochimie CNRS UMR 7654, Ecole Polytechnique, Palaiseau, France
    Eur J Biochem 267:4789-98. 2000
    ..These reagents were used for qualitative comparative labeling of Escherichia coli valyl-tRNA synthetase (ValRS), an enzyme with Val/Ile editing activity, in order to identify the binding ..
  27. ncbi Modulation of serum cytokine levels by a novel superoxide dismutase mimetic, M40401, in an Escherichia coli model of septic shock: correlation with preserved circulating catecholamines
    Heather Macarthur
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, MO, USA
    Crit Care Med 31:237-45. 2003
    ..Here we examined if maintenance of serum catecholamine levels by the superoxide dismutase mimetic, M40401, modulates serum cytokine levels and arterial hypotension in an Escherichia coli-infected conscious rat model of septic shock.
  28. doi Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes
    Thi Thu Hao Van
    Biotechnology and Environmental Biology, School of Applied Sciences, RMIT University, Bundoora West Campus, Bundoora, Melbourne, VIC 3083, Australia
    Int J Food Microbiol 124:217-23. 2008
    This study was conducted to examine a current baseline profile of antimicrobial resistance and virulence of Escherichia coli isolated from foods commonly sold in the market place in Vietnam. E...
  29. ncbi Prevalent phenotypes and antibiotic resistance in Escherichia coli and Klebsiella pneumoniae at an Indian tertiary care hospital: plasmid-mediated cefoxitin resistance
    M Shahid
    Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
    Int J Infect Dis 12:256-64. 2008
    ..Due to increased transcontinental movement of the human population, it would be wise to know the prevalence and resistance complexity of these strains, well in advance, in order to formulate a policy for empirical therapy...
  30. ncbi Comparison between the growth of Yersinia enterocolitica, listeria monocytogenes, escherichia coli O157:H7 and salmonella spp. in ground beef packed by three commercially used packaging techniques
    H Nissen
    MATFORSK, Norwegian Food Research Institute, Oslovn
    Int J Food Microbiol 59:211-20. 2000
    Growth of the pathogens Yersinia enterocolitica, Listeria monocytogenes, Escherichia coli O157:H7 and strains of Salmonella were compared in ground beef packed in modified atmospheres of 60% CO2/40% N2/0...
  31. ncbi The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli
    M Terrak
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6, B 4000 Sart Tilman Liège, Belgium
    Mol Microbiol 34:350-64. 1999
    The penicillin-binding protein (PBP) 1b of Escherichia coli catalyses the assembly of lipid-transported N-acetyl glucosaminyl-beta-1, 4-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelyl+ ++- (L)-D-alanyl-D-alanine ..
  32. doi Heterogeneity in enterohemorrhagic Escherichia coli O157:H7 fecal shedding in cattle is related to Escherichia coli O157:H7 colonization of the small and large intestine
    Danica Baines
    Lethbridge Research Centre, Agriculture and Agri Food Canada, Lethbridge, ABT1J4B1, Canada
    Can J Microbiol 54:984-95. 2008
    In the last decade, Escherichia coli O157:H7 have emerged as important pathogens of the gastrointestinal tract of humans. Healthy cattle have been identified as the primary reservoir, however, the factors affecting heterogeneous E...
  33. ncbi Comparison of Escherichia coli and Klebsiella pneumoniae liver abscesses
    Shiuan Chih Chen
    Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
    Am J Med Sci 334:97-105. 2007
    b>Escherichia coli and Klebsiella pneumoniae are the most common causative pathogens of pyogenic liver abscesses...
  34. ncbi Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium
    Mohammad Mohsenzadeh
    Department of Food Hygiene and Public Health, Ferdowsi University of Mashhad, P O Box 91775 1793, Mashhad, Iran
    Pak J Biol Sci 10:3693-7. 2007
    ..Artmesia dracunculus) and pennyroyal (Mentha pullegium) essential oils on the Staphylococcus aureus and Escherichia coli was studied in nutrient broth medium...
  35. doi Evaluation of fermentation, drying, and/or high pressure processing on viability of Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., and Trichinella spiralis in raw pork and Genoa salami
    Anna C S Porto-Fett
    U S Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Microbial Food Safety Research Unit, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Int J Food Microbiol 140:61-75. 2010
    ..of fermentation, drying, and high pressure processing (HPP) to inactivate Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., and Trichinella spiralis in Genoa salami produced with trichinae-infected pork...
  36. doi Effects of mucin and its carbohydrate constituents on Escherichia coli O157 growth in batch culture fermentations with ruminal or fecal microbial inoculum
    J T Fox
    Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan 66506, USA
    J Anim Sci 87:1304-13. 2009
    In cattle, Escherichia coli O157 generally persists in the large intestine more often than in the rumen. In contrast to the rumen, the large intestine is lined by an epithelial membrane that secretes mucus...
  37. ncbi Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase
    S Boschi-Muller
    Laboratoire d Enzymologie et de Génie Génétique, Universite de Nancy I, URA CNRS 457, B P 239, 54506 Vandoeuvre les Nancy Cedex, France
    J Biol Chem 272:15106-12. 1997
    GapB-encoded protein of Escherichia coli and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) share more than 40% amino acid identity...
  38. ncbi Binding of Escherichia coli primary replicative helicase DnaB protein to single-stranded DNA. Long-range allosteric conformational changes within the protein hexamer
    M J Jezewska
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 1053, USA
    Biochemistry 35:2129-45. 1996
    Quantitative analyses of the interactions of the Escherichia coli primary replicative helicase DnaB protein with single-stranded DNA have been performed using the thermodynamically rigorous fluorescence titration technique...
  39. ncbi Effective medicinal plants against enterohaemorrhagic Escherichia coli O157:H7
    Supayang Voravuthikunchai
    School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia
    J Ethnopharmacol 94:49-54. 2004
    ..of subinhibitory concentrations of antibiotics on the production of verocytotoxin (VT) by enterohaemorrhagic Escherichia coli (EHEC) O157:H7 has been claimed...
  40. ncbi Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
    G Unden
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Biochim Biophys Acta 1320:217-34. 1997
    The electron-transport chains of Escherichia coli are composed of many different dehydrogenases and terminal reductases (or oxidases) which are linked by quinones (ubiquinone, menaquinone and demethylmenaquinone)...
  41. ncbi Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis
    G Waksman
    Laboratory of Molecular Biophysics, Rockefeller University, New York, NY 10021
    J Mol Biol 236:800-16. 1994
    The crystal structures of three forms of Escherichia coli thioredoxin reductase have been refined: the oxidized form of the wild-type enzyme at 2...
  42. pmc Polyamine stress at high pH in Escherichia coli K-12
    Elizabeth Yohannes
    Department of Biology, Kenyon College, Gambier, OH 43022, USA
    BMC Microbiol 5:59. 2005
    Polyamines such as spermine and spermidine are required for growth of Escherichia coli; they interact with nucleic acids, and they bind to ribosomes...
  43. ncbi A comparison of mutation spectra detected by the Escherichia coli lac(+) reversion assay and the Salmonella typhimurium his(+) reversion assay
    T Ohta
    School of Life Science, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Mutagenesis 15:317-23. 2000
    Each of the Escherichia coli tester strains in the WP3101P-WP3106P series contains an F' plasmid with a different base substitution mutation within the lacZ gene...
  44. ncbi [Selective screening of enterohemorrhagic Escherichia coli depending on hemolysis on Beutin's washed sheep blood agar plates]
    N Kimura
    Ishikawa Health Service Association
    Kansenshogaku Zasshi 72:223-30. 1998
    ..et al have studied hemolysin production in a large number of serologically diverse verotoxin (VT) producing Escherichia coli (VTEC) strains and found a close association between hemolysin production and VT production...
  45. ncbi Mode of selectivity in cyclic AMP receptor protein-dependent promoters in Escherichia coli
    E A Pyles
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77556 1055, USA
    Biochemistry 35:1162-72. 1996
    b>Escherichia coli cAMP receptor protein (CRP) controls more than 20 genes. There are significant differences in the promoter regions in these genes...
  46. ncbi Kinetic mechanism of DNA binding and DNA-induced dimerization of the Escherichia coli Rep helicase
    K P Bjornson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 35:2268-82. 1996
    The monomeric Escherichia coli Rep protein undergoes a DNA-induced dimerization upon binding either single-stranded (ss) or duplex DNA with the dimer being the active form of the Rep helicase...
  47. ncbi Microbial source tracking by DNA sequence analysis of the Escherichia coli malate dehydrogenase gene
    Kathryn M Ivanetich
    University of California San Francisco, Biomolecular Resource Center and Department of Pharmaceutical Chemistry, Surge 104, 90 Medical Center Way, San Francisco, CA 94143 0541, USA
    J Microbiol Methods 67:507-26. 2006
    ..of microbial organisms by DNA sequencing proposed by Olive and Bean were applied to several genes in Escherichia coli to identify targets for the development of microbial source tracking assays...
  48. ncbi Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range
    Zhili Li
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Anal Biochem 343:35-47. 2005
    b>Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of phosphoenolpyruvate (PEP) and D-arabinose 5-phosphate (A5P) to produce KDO8P and inorganic phosphate...
  49. doi Perineal swabs reveal effect of super shedders on the transmission of Escherichia coli O157:H7 in commercial feedlots
    T P Stephens
    Alberta Agriculture and Rural Development, Agriculture Centre, 100, 5401 1st Avenue South, Lethbridge, Alberta, Canada T1J 4V6
    J Anim Sci 87:4151-60. 2009
    Cattle that shed more than 10(4) cfu/g of Escherichia coli O157 in feces have been described as super shedders (SS) and are thought to have major impacts on prevalence and transmission of this organism...
  50. ncbi Hemolytic-Uremic Syndrome and Escherichia coli O121 at a Lake in Connecticut, 1999
    T A McCarthy
    Epidemic Intelligence Service, Connecticut Department of Public Health, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Pediatrics 108:E59. 2001
    Non-O157 Shiga toxin-producing Escherichia coli (STEC) have emerged as an important public health problem. Outbreaks attributed to non-O157 STEC rarely are reported...
  51. ncbi Evaluation of techniques for enrichment and isolation of Escherichia coli O157:H7 from artificially contaminated sprouts
    S D Weagant
    Pacific Regional Laboratory Northwest, US Food and Drug Administration, Bothell, WA 98021, USA
    Int J Food Microbiol 71:87-92. 2001
    ..have high levels of nutrients, and a neutral pH, they are subject to the outgrowth of pathogens such as Escherichia coli O157:H7...
  52. pmc Impact of empiric antimicrobial therapy on outcomes in patients with Escherichia coli and Klebsiella pneumoniae bacteremia: a cohort study
    Kerri A Thom
    Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, MD, USA
    BMC Infect Dis 8:116. 2008
    ..whether appropriate empiric antimicrobial therapy improves outcomes in patients with bacteremia due to Escherichia coli or Klebsiella...
  53. ncbi Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar
    T P McDonald
    Department of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom
    J Biol Chem 272:15189-99. 1997
    The galactose-H+ symport protein (GalP) of Escherichia coli is very similar to the human glucose transport protein, GLUT1, and both contain a highly conserved Asn residue in predicted helix 11 that is different in a cytochalasin B-..
  54. ncbi [Antibacterial actin of vinegar against food-borne pathogenic bacteria including Escherichia coli O157:H7 (Part 2). Effect of sodium chloride and temperature on bactericidal activity]
    E Entani
    Nakano Central Research Institute of Nakano Vinegar Co. Ltd
    Kansenshogaku Zasshi 71:451-8. 1997
    ..5% acidity) on food-borne pathogenic bacteria including Escherichia coli O157:H7 and other bacteria were examined. the order of bactericidal activities was NIHAIZU (3...
  55. pmc Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain
    Germán L Rosano
    Molecular Biology Division, Instituto de Biologia Molecular y Celular de Rosario IBR, CONICET, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina
    Microb Cell Fact 8:41. 2009
    ..abstract:..
  56. ncbi Prevalence of enteropathogenic Escherichia coli in naturally occurring cases of poult enteritis-mortality syndrome
    S Pakpinyo
    Department of Farm Animal Health and Resource Management, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA
    Avian Dis 46:360-9. 2002
    Enteropathogenic Escherichia coli (EPEC) previously were identified in poult enteritis-mortality syndrome (PEMS)-affected turkeys and associated as a cause of this disease...
  57. doi Growth performance and gastrointestinal microbial ecology responses of piglets receiving Saccharomyces cerevisiae fermentation products after an oral challenge with Escherichia coli (K88)
    E Kiarie
    Department of Animal Science, Winnipeg, Manitoba R3T 2N2, Canada
    J Anim Sci 89:1062-78. 2011
    ..on growth performance and gastrointestinal (GIT) microbial ecology in 90 weanling pigs orally challenged with Escherichia coli K88(+) (ETEC) were investigated...
  58. ncbi [Investigation of bactericidal effect and nitric oxide responses of Caco-2 epithelial cells and THP-1 macrophage cells against Streptococcus pyogenes and Escherichia coli]
    Derya Biriken Salin
    Ankara Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Ankara
    Mikrobiyol Bul 43:373-81. 2009
    ..Therefore, Streptococcus pyogenes and Escherichia coli activated Caco-2 cells and THP-1 macrophage-like cells were investigated for their bactericidal activities ..
  59. ncbi On the multiple functional roles of the active site histidine in catalysis and allosteric regulation of Escherichia coli glucosamine 6-phosphate deaminase
    G M Montero-Morán
    Departamento de Bioquimica, Laboratorio de Fisicoquímica y Diseño de Proteínas, Facultad de Medicina, Universidad Nacional Autonoma de Mexico UNAM, P O Box 70 159, Mexico City 04510, D F, Mexico
    Biochemistry 40:10187-96. 2001
    The active site of glucosamine-6-phosphate deaminase (EC 3.5.99.6, formerly 5.3.1.10) from Escherichia coli was first characterized on the basis of the crystallographic structure of the enzyme bound to the competitive inhibitor 2-amino-2-..
  60. ncbi Quantitative resistance level (MIC) of bacterial pathogens (Escherichia coli, Pasteurella multocida, Pseudomonas aeruginosa, Salmonella sp., Staphylococcus aureus) isolated from chickens and turkeys: national resistance monitoring by the BVL 2004/2005
    Jürgen Wallmann
    Federal Office of Consumer Protection and Food Safety BVL, Berlin, Germany
    Berl Munch Tierarztl Wochenschr 120:452-63. 2007
    In the study 2004/2005, the current quantitative resistance level of Escherichia coli, Pasteurella multocida, Pseudomonas aeruginosa, Salmonella sp...
  61. ncbi Influence of colostrum deprivation and concurrent Cryptosporidium parvum infection on the colonization and persistence of Escherichia coli O157 : H7 in young lambs
    R M La Ragione
    Department of Food and Environmental Safety, Veterinary Laboratories Agency Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    J Med Microbiol 55:819-28. 2006
    b>Escherichia coli O157 : H7 and Cryptosporidium parvum infections of man have been associated with direct contact with small ruminants...
  62. doi Modeling the survival of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium during fermentation, drying, and storage of soudjouk-style fermented sausage
    Cheng An Hwang
    Microbial Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 E Mermaid Lane, Wyndmoor, PA 19038, USA
    Int J Food Microbiol 129:244-52. 2009
    This study quantified and modeled the survival of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Typhimurium in soudjouk-style fermented sausage during fermentation, drying, and storage...
  63. ncbi Role of Asp222 in the catalytic mechanism of Escherichia coli aspartate aminotransferase: the amino acid residue which enhances the function of the enzyme-bound coenzyme pyridoxal 5'-phosphate
    T Yano
    Department of Medical Chemistry, Osaka Medical College, Japan
    Biochemistry 31:5878-87. 1992
    ..This residue of Escherichia coli aspartate aminotransferase was replaced by Ala, Asn, or Glu by site-directed mutagenesis...
  64. ncbi RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes
    B Krummel
    Department of Biochemistry, University of California, Berkeley 94720
    Biochemistry 28:7829-42. 1989
    We have studied the properties and structures of a series of Escherichia coli RNA polymerase ternary complexes formed during the initial steps of RNA chain initiation and elongation...
  65. ncbi Mechanism of carbamoyl phosphate synthetase from Escherichia coli--binding of the ATP molecules used in the reaction and sequestration by the enzyme of the ATP molecule that yields carbamoyl phosphate
    V Rubio
    Instituto de Investigaciones Citológicas FVIB CSIC, Valencia, Spain
    Eur J Biochem 255:262-70. 1998
    ..of ATP that are involved in the overall reaction catalyzed by carbamoyl-phosphate synthetase (CPS) of Escherichia coli, and a mechanism recently proposed for this reaction, has led us to reexamine ATP binding using pulse/chase ..
  66. pmc Evaluation of the NCCLS extended-spectrum beta-lactamase confirmation methods for Escherichia coli with isolates collected during Project ICARE
    Fred C Tenover
    Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 41:3142-6. 2003
    To determine whether confirmatory tests for extended-spectrum beta-lactamase (ESBL) production in Escherichia coli are necessary, we selected 131 E...
  67. doi Transport of Escherichia coli in 25 m quartz sand columns
    G Lutterodt
    UNESCO IHE Institute for Water Education, P O Box 3015, 2601 DA Delft, The Netherlands
    J Contam Hydrol 119:80-8. 2011
    ..experiments were conducted to measure the distant dependent sticking efficiencies of two low attaching Escherichia coli strains (UCFL-94 and UCFL-131)...
  68. ncbi Influence of dietary endophyte (Neotyphodium coenophialum)-infected tall fescue (Festuca arundinacea) seed on fecal shedding of antibiotic resistance-selected Escherichia coli O157:H7 in ewes
    M L Looper
    USDA ARS, Dale Bumpers Small Farms Research Center, Booneville, AR 72927, USA
    J Anim Sci 85:1102-8. 2007
    ..fescue seed (Festuca arundinacea, cultivar 'Kentucky 31') on fecal shedding and intestinal concentrations of Escherichia coli O157:H7 and the concentrations of prolactin, cortisol, and NEFA in experimentally inoculated ewes...
  69. ncbi Kinetic mechanism of adenine nucleotide binding to and hydrolysis by the Escherichia coli Rep monomer. 2. Application of a kinetic competition approach
    K J Moore
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    Biochemistry 33:14565-78. 1994
    The Escherichia coli Rep protein is a DNA helicase that functions as a homodimer to catalyze the unwinding of duplex DNA during DNA replication in a reaction that is coupled to the binding and hydrolysis of ATP...
  70. doi Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: efficacy and mode of action
    A F Pedroso
    Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32606, USA
    J Dairy Sci 93:1098-104. 2010
    This study aimed to evaluate the effectiveness of 3 commercial bacterial inoculants at controlling Escherichia coli O157:H7 in corn silages during ensiling and feedout phases of silage production...
  71. pmc Serotypes, virulence genes and intimin types of Shiga toxin (verocytotoxin)-producing Escherichia coli isolates from minced beef in Lugo (Spain) from 1995 through 2003
    Azucena Mora
    Laboratorio de Referencia de E coli, Departamento de Microbioloxia e Parasitoloxia, Universidade de Santiago de Compostela USC, 27002 Lugo, Spain
    BMC Microbiol 7:13. 2007
    Shiga toxin-producing Escherichia coli (STEC) have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans, such as haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS)...
  72. ncbi [Evolution of Escherichia coli antibiotic resistances in urine samples from the community]
    Jose Maria Sanchez Merino
    Servicio de Urologia, Hospital El Bierzo, Ponferrada, Leon, Espana
    Arch Esp Urol 61:776-80. 2008
    The objectives of this work are two: first, to evaluate the resistance of Escherichia coli to several antibiotics and their trends over a six-year period in strands isolated in urine samples from patients receiving health-care in general ..
  73. ncbi Escherichia coli lipoyl synthase binds two distinct [4Fe-4S] clusters per polypeptide
    Robert M Cicchillo
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 43:11770-81. 2004
    ..However, the exact identity of the sulfur donor remains unknown. We report herein that LS from Escherichia coli can accommodate two [4Fe-4S] clusters per polypeptide and that this form of the enzyme is relevant to ..
  74. ncbi Differential effects of temperature and hydrostatic pressure on the formation of quinonoid intermediates from L-Trp and L-Met by H463F mutant Escherichia coli tryptophan indole-lyase
    Robert S Phillips
    Department of Chemistry, University of Georgia, Athens, Georgia 30602 2556, USA
    Biochemistry 44:14289-97. 2005
    b>Escherichia coli tryptophan indole-lyase (Trpase) is a bacterial pyridoxal 5'-phosphate (PLP)-dependent enzyme which catalyzes the reversible beta-elimination of l-Trp to give indole and ammonium pyruvate. H463F mutant E...
  75. ncbi Multiple seropathotypes of verotoxin-producing Escherichia coli (VTEC) disrupt interferon-gamma-induced tyrosine phosphorylation of signal transducer and activator of transcription (Stat)-1
    Narveen Jandu
    Research Institute, Hospital for Sick Children, Ont, Canada
    Microb Pathog 42:62-71. 2007
    Verotoxin-producing Escherichia coli (VTEC) O157:H7 inhibits interferon-gamma-stimulated tyrosine phosphorylation of signal transducer and activator of transcription (Stat)-1 in epithelial cells, independent of Verotoxins and the locus of ..
  76. pmc Mapping of RNA polymerase residues that interact with bacteriophage Xp10 transcription antitermination factor p7
    Yulia Yuzenkova
    Waksman Institute for Microbiology Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Mol Biol 375:29-35. 2008
    ..P7 does not bind to and has no effect on RNA polymerase from Escherichia coli. Here, we use a combination of biochemical and genetic methods to map the p7 interaction site to within four ..
  77. pmc The TFPI-2 derived peptide EDC34 improves outcome of gram-negative sepsis
    Praveen Papareddy
    Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, Biomedical Center, Lund, Sweden
    PLoS Pathog 9:e1003803. 2013
    ..Correspondingly, in mouse models of severe Escherichia coli and Pseudomonas aeruginosa infection, treatment with EDC34 reduced bacterial levels and lung damage...
  78. doi Two fluorogenic substrates for purine nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme
    Jacek Wierzchowski
    Department of Biophysics, University of Varmia and Masuria, 10 719 Olsztyn, Poland Electronic address
    Anal Biochem 446:25-7. 2014
    ..have been found to exhibit moderately good, and selective, substrate properties toward calf and bacterial (Escherichia coli) forms of purine nucleoside phosphorylase (PNP)...
  79. doi The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14
    Christian Pustelny
    Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany
    Environ Microbiol 15:597-609. 2013
    ..aeruginosa. We focused the work on a protein named HemK with high homology to PrmC of Escherichia coli displaying a similar enzymatic activity (therefore also referred to as PrmC)...
  80. pmc Divergent evolution of ligand binding in the o-succinylbenzoate synthase family
    Denis Odokonyero
    Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, Texas 77843 2128, United States
    Biochemistry 52:7512-21. 2013
    ..amino acid sequence that is 22 and 28% identical with those of two previously characterized OSBS enzymes from Escherichia coli and Amycolatopsis sp. T-1-60, respectively...
  81. doi Characterization and in vitro inhibition studies of Bacillus anthracis FtsZ: a potential antibacterial target
    Hae Chul Park
    Department of Chemistry and Institute of Natural Science, Hanyang University, Seoul, 133 791, Republic of Korea
    Appl Biochem Biotechnol 172:3263-70. 2014
    ..study, the FtsZ of Bacillus anthracis was cloned into a bacterial expression vector and overexpressed into Escherichia coli BL21 (DE3) cells. The overexpressed B...
  82. doi Antimicrobial resistance and class 1 and 2 integrons in Escherichia coli from meat turkeys in Northern Italy
    A Piccirillo
    a Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy
    Avian Pathol 43:396-405. 2014
    ..the antimicrobial resistance (AMR) and the presence of class 1 and 2 integrons in 48 avian pathogenic Escherichia coli (APEC) strains isolated from meat turkeys during three sequential production cycles. Thirty avian faecal E...
  83. doi Cloning, expression and biochemical characterization of mitochondrial and cytosolic malate dehydrogenase from Phytophthora infestans
    Patricia E López-Calcagno
    Laboratorio de Fisiologia, Facultad de Ciencias, Universidad de Los Andes, Merida, Venezuela
    Mycol Res 113:771-81. 2009
    ..and cytosolic malate dehydrogenase (mMDH and cMDH) of Phytophthora infestans were cloned and overexpressed in Escherichia coli as active enzymes...
  84. pmc Crystal structure of Thermobifida fusca Cse1 reveals target DNA binding site
    Melanie Tay
    Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, Singapore, 117543, Singapore
    Protein Sci 24:236-45. 2015
    ..Moreover, structural docking of T. fusca Cse1 into Escherichia coli Cascade cryoelectron microscopy maps, coupled with structural comparison, reveals a conserved positive patch ..
  85. ncbi Synthesis and characterization of dextran-capped silver nanoparticles with enhanced antibacterial activity
    Guili Yang
    Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center Academy of Military Medical Sciences, Beijing 100850, China
    J Nanosci Nanotechnol 12:3766-74. 2012
    ..Antibacterial tests of these silver nanoparticles were carried out for Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Klebsiella pneumoniae...
  86. doi Two Lactococcus lactis thioredoxin paralogues play different roles in responses to arsenate and oxidative stress
    Petr Efler
    Enzyme and Protein Chemistry, Søltofts Plads Building 224, Department of Systems Biology, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Microbiology 161:528-38. 2015
    ..TrxA is similar to the well-characterized Trx homologue from Escherichia coli and contains the common WCGPC active site motif, while TrxD is atypical and contains an aspartate residue in ..
  87. pmc Single-particle tracking for DNA tether length monitoring
    Noëlle Pouget
    Institut de Pharmacologie et Biologie Structurale UMR CNRS 5089, 205 route de Narbonne, 31077 Toulouse Cedex, France
    Nucleic Acids Res 32:e73. 2004
    ..by the evidence obtained for conformational changes introduced into a Holliday junction by the binding of the Escherichia coli RuvA protein...
  88. doi Improved biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by adding L-glutamine plus glycine instead of NAD+ in β-cyclodextrin-water system
    Yu Cai He
    Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China Electronic address
    Bioresour Technol 182:98-102. 2015
    ..was also added into this reaction media, and the biocatalytic activity of the whole-cell biocatalyst of Escherichia coli CCZU-K14 was increased by 1.34-fold than that without β-cyclodextrin...
  89. doi Channel characteristics of VDAC-3 from Arabidopsis thaliana
    C Berrier
    Neuroscience Paris Saclay Institute Neuro PSI UMR 9197, CNRS Universite Paris Sud, Batiment 430, F 91405 Orsay cedex, France
    Biochem Biophys Res Commun 459:24-8. 2015
    ..One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured...
  90. ncbi Cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from Serratia proteamaculans representing a new group of thermolysin-like proteases with short N-terminal region of precursor
    Ilya V Demidyuk
    Laboratory of Protein Engineering, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
    Protein Expr Purif 47:551-61. 2006
    The gene of Serratia proteamaculans proteinase, protealysin, was cloned, sequenced, and expressed in Escherichia coli. The gene encoded a precursor of 341 amino acids (AAs) with a significant homology to thermolysin-like proteinases (TLPs)..
  91. pmc Engineered recombinant human paraoxonase 1 (rHuPON1) purified from Escherichia coli protects against organophosphate poisoning
    Richard C Stevens
    Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195 7720, USA
    Proc Natl Acad Sci U S A 105:12780-4. 2008
    ..Expression of active recombinant human PON1 (rHuPON1) in Escherichia coli provides a system in which PON1 can be engineered to achieve a catalytic efficiency sufficient to protect ..
  92. ncbi NikA binds heme: a new role for an Escherichia coli periplasmic nickel-binding protein
    Mark Shepherd
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Biochemistry 46:5030-7. 2007
    NikA is a periplasmic binding protein involved in nickel uptake in Escherichia coli. NikA was identified as a heme-binding protein in the periplasm of anaerobically grown cells overexpressing CydDC, an ABC transporter that exports ..
  93. doi Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus
    Ana Carolina Magalhães Andrade de Góes
    Biopharmaceuticals Program, Bio Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    J Virol Methods 148:211-7. 2008
    ..A LAT, part of segment 6 corresponding to conserved N-terminal portion of the VP6 (1-245 aa) was cloned in Escherichia coli expression pGEX vector (glutathione S-transferase-GST gene fusion system) that has been modified previously ..
  94. doi Preparation and properties of dextran sulfate-lysozyme conjugate
    Zahra Alahdad
    Department of Food Science and Technology, School of Agriculture
    J Agric Food Chem 57:6449-54. 2009
    ..conjugate showed antibacterial activity and significantly inhibited growth of Staphylococcus aureus and Escherichia coli in a dose-dependent manner...
  95. pmc Massive formation of intracellular membrane vesicles in Escherichia coli by a monotopic membrane-bound lipid glycosyltransferase
    Hanna M Eriksson
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 106 91 Stockholm, Sweden
    J Biol Chem 284:33904-14. 2009
    ..Strong overexpression in Escherichia coli of a foreign monotopic glycosyltransferase (named monoglycosyldiacylglycerol synthase), synthesizing a ..
  96. doi Response surface methodology for process parameter optimization of hydrogen yield by the metabolically engineered strain Escherichia coli DJT135
    Dipankar Ghosh
    Departement de Microbiologie et Immunologie, Universite de Montreal, CP 6128 Succursale Centre Ville, Montreal, Quebec, Canada
    Bioresour Technol 101:1820-5. 2010
    ..conditions; pH, temperature and glucose concentration, on the molar hydrogen yield by a genetically optimized Escherichia coli strain, DJT135...
  97. doi X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate
    Kalyan Sundar Chakrabarti
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    FEBS J 277:697-712. 2010
    ..The tertiary structure of the dimeric N-terminal domain of Escherichia coli pantothenate synthetase, determined by X-ray crystallography to a resolution of 1...
  98. doi Rapid and sensitive DNA target detection using enzyme amplified electrochemical detection based on microchip
    Wei Cao
    College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, P R China
    Electrophoresis 31:659-65. 2010
    ..2 x 10(-11) M, respectively. The proposed method has been applied satisfactorily in the analysis of Escherichia coli genomic DNA. We selected the detection of PCR amplifications from the gene of E...
  99. pmc Mechanism of RecO recruitment to DNA by single-stranded DNA binding protein
    Mikhail Ryzhikov
    Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, 1100 S Grand Blvd, St Louis, MO 63021, USA
    Nucleic Acids Res 39:6305-14. 2011
    ..We present a crystal structure of Escherichia coli RecO bound to the conserved SSB C-terminus (SSB-Ct)...
  100. doi Stable integration of an engineered megabase repeat array into the maize genome
    Han Zhang
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Plant J 70:357-65. 2012
    ..We conclude that synthetic repeats can be delivered into plant cells by omitting passage through Escherichia coli, that they generally insert into one locus, and that great lengths may be achieved...
  101. pmc Genetic engineering of the phosphocarrier protein NPr of the Escherichia coli phosphotransferase system selectively improves sugar uptake activity
    Yossef Lopez-de Los Santos
    Departamento de Ingeniería Celular y Biocatálisis del Instituto de Biotecnología, Universidad Nacional Autonoma de Mexico, Avenue Universidad 2001, Cuernavaca, Morelos, Mexico, P C 62210
    J Biol Chem 287:29931-9. 2012
    The Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system (PTS) in prokaryotes mediates the uptake and phosphorylation of its numerous substrates through a phosphoryl transfer chain where a phosphoryl transfer protein, HPr, ..

Research Grants86

  1. S. typhimiurium Vaccine Against Bacterial Enteropathogens
    Roy Curtiss; Fiscal Year: 2007
    ..2 million are the result of infections by Salmonella and other related bacterial enteropathogens including Escherichia coli and Shigella species, and less closely related enteropathogens such as Vibrio cholerae, Campylobacter jejuni ..
  2. Stomate-based innate immunity against bacterial infection in Arabidopsis
    Maeli Melotto; Fiscal Year: 2012
    ..Specifically, stomata close in response to Escherichia coli O157:H7 and Pseudomonas syringae pv. tomato (Pst) DC3000 or bacterial PAMPs...
  3. Salmonella Vaccines Against Bacterial Enteropathogens
    Roy Curtiss; Fiscal Year: 2013
    ..8 (WHO) to 2.2 (UNESCO) million are due to infections with enteric pathogens including Salmonella, Escherichia coli and Shigella sp., and other entero-pathogens such as Yersinia sp...
  4. Biological Effects of DNA Adducts Formed by Nitroaromatic Compounds
    Zucai Suo; Fiscal Year: 2013
    ..to construct single-stranded plasmid or viral vectors and mutagenicity and genotoxicity will be evaluated in Escherichia coli and mammalian cells...
  5. The function of small RNA-based viral defense system in E. coli
    KONSTANTIN V SEVERINOV; Fiscal Year: 2013
    ..Neither stage of CRISPR response is fully understood. We propose to study CRISPR function in Escherichia coli, the best-studied prokaryote. CRISPR/cas loci of laboratory E. coli are dormant...
  6. Cellular functions of the essential PcrA helicase in Staphylococcus aureus
    Saleem A Khan; Fiscal Year: 2013
    ..PcrA is related to the Escherichia coli UvrD and Rep helicases and is known to be essential for UV DNA repair in Bacillus subtilis. In B...
  7. DNA-Binding Protein Discovery by the Combinatorial Method REPSA
    Michael van Dyke; Fiscal Year: 2013
    ..For example, while the bacteria Escherichia coli strain K12 has 4364 open reading frames, only about half of these genes have been well characterized by ..
  8. GENETIC ANALYSIS OF PROTEIN EXPORT
    Thomas J Silhavy; Fiscal Year: 2013
    ..of this grant has always been to understand the mechanisms of envelope biogenesis in molecular terms using Escherichia coli as a model system...
  9. Single-molecule visualization of transcription regulation mechanisms
    Jeff Gelles; Fiscal Year: 2013
    ..selected processes involved in regulation of transcription initiation and elongation in vitro using purified Escherichia coli proteins in reconstituted transcription reactions...
  10. Novel Bioconjugate Vaccines to Prevent Staphylococcus aureus Infection
    JEAN CLAIRE LEE; Fiscal Year: 2013
    ..This protein glycosylation system has been functionally transferred into Escherichia coli, and it allows the conjugation of an antigenic polysaccharide to a designer protein of choice through an N-..
  11. Interactions of Enteropathogenic E Coli with Intestinal Epithelial Cells
    Gail A Hecht; Fiscal Year: 2013
    ..Enteropathogenic Escherichia coli (EPEC) is a foodborne non-invasive pathogen that causes diarrhea...
  12. Emergence of E. coli sequence type ST131
    James R Johnson; Fiscal Year: 2013
    A specific group of Escherichia coli, sequence type ST131 is a newly recognized, widely disseminated cause of multi-drug-resistant (MDR extraintestinal infections that appears to represent an important emerging health threat in urgent ..
  13. Molecular Pathogenesis of Enterotoxigenic Escherichia coli Infections
    JAMES MICHAEL FLECKENSTEIN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The enterotoxigenic Escherichia coli (ETEC) are a genetically diverse type of pathogenic E...
  14. Production of Activated TDP-Deoxysugars in E. coli
    MICAH SHEPHERD; Fiscal Year: 2012
    ..We propose to investigate a novel approach to produce rare TDP-deoxysugars in Escherichia coli through metabolic engineering...
  15. Toxoid adjuvant CRM197 production in a stable reduced genome E. coli strain
    Frederick R Blattner; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Scarab Genomics has developed valuable reduced-genome strains of Escherichia coli in which multiple deletions remove from the genome much unwanted and all potentially hazardous DNA...
  16. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    Pei Zhou; Fiscal Year: 2012
    ..b>Escherichia coli lipid A is synthesized on the cytoplasmic surface of the inner membrane by a conserved pathway of nine ..
  17. Molecular analysis of oriT-independent transfer in the gonococcal R-plasmids
    LUIS TORRES-BAUZA; Fiscal Year: 2012
    ..We found that the 5.2 kb R- plasmid isolated in San Juan, pSJ5.2, can be mobilized to Escherichia coli by fusion (cointegration) with the help of the Enterobacterial R64, N3 and the 41 kb tet(M) conjugative ..
  18. Colitis Induced by immune responses to luminal bacteria-mouse
    Ryan B Sartor; Fiscal Year: 2012
    ..Selective colonization of germ-free IL-10-/- mice with Escherichia coli or Enterococcus faecalis induces different phenotypes of colitis for each bacteria. E. coli and E...
  19. 4th ASM Conference on Prokaryotic Cell Biology and Development
    Susan S Golden; Fiscal Year: 2012
    ..The core subject matter involves five widely and successfully studied systems: Escherichia coli (cell division, cytoskeletal structures, cell wall biosynthesis), Bacillus subtilis (cell cycle regulation, ..
  20. Fatty acid related regulation of enteric infectious disease
    FREDRICK JON KULL; Fiscal Year: 2012
    ..Preliminary analysis has indicated that a number of pathogenic bacteria, including several types of Escherichia coli, Salmonella typhi, Salmonella typhimurium, Shigella flexneri, and Yersinia enterocolitica, contain A/X ..
  21. Rho-Modifying Cytotoxic Necrotizing Factor of E. coli
    ALISON DAVIS O'BRIEN; Fiscal Year: 2013
    ..CNF1 and a membrane-lytic toxin, hemolysin (Hly), are often co- expressed by Escherichia coli strains that cause urinary tract infections (UTIs), i.e., cystitis or pyelonephritis, or acute prostatitis...
  22. The Electricidal Effect, a Novel Anti-Biofilm Strategy
    Robin Patel; Fiscal Year: 2013
    ..aeruginosa, S. aureus, and S. epidermidis, three isolates each of Escherichia coli and Enterococcus faecalis, and single isolates of each of Streptococcus mutans species group and Candida ..
  23. Bacterial Stress Responses to Intestinal Inflammation
    JONATHAN JAMES HANSEN; Fiscal Year: 2013
    ..Isolates of Escherichia coli (E...
  24. Stochastic Partitioning and Degradation of Macromolecules
    Johan Paulsson; Fiscal Year: 2013
    ..for different types of macromolecules: in-depth analyses of active segregation mechanisms of plasmids in Escherichia coli, a quantification of proteolysis in E. coli, and an mRNA and protein survey in Schizosaccharomyces pombe...
  25. Structure and mechanism of CRISPR interference.
    Ailong Ke; Fiscal Year: 2013
    ..far sequenced, including important human pathogens such as Campylobacter human jejuni, Clostridium botulinum, Escherichia coli, Listeria monocytogenes, Mycobacterium tuberculosis and Yersinia pestis...
  26. Bacterial cell killing by topoisomerase I mediated DNA lesion
    Yuk Ching Tse-Dinh; Fiscal Year: 2013
    ..Priority would be given to compounds with low toxicity that are active against gram negative Escherichia coli and Yersinia pestis in order to identify leads that can be used in development of therapeutic agents against ..
  27. ADHESIN-BASED NANOTHERAPEUTICS IN URINARY TRACT INFECTION
    DAVID ALAN HUNSTAD; Fiscal Year: 2013
    ..UTIs), a major source of morbidity and medical costs worldwide, are caused primarily by uropathogenic Escherichia coli, which employ an adhesive fiber termed the type 1 pilus to bind and invade bladder epithelial cells...
  28. Molecular Basis of E. coli Adhesins in Bladder Disorders
    Scott J Hultgren; Fiscal Year: 2013
    ..S., ~85% of which are caused by uropathogenic Escherichia coli (UPEC)...
  29. Structural basis for the assembly of the Sec translocase machinery
    CHARALAMPOS KALODIMOS; Fiscal Year: 2013
    ..We have extensively characterized over the last years all of the protein components from Escherichia coli and have obtained and present here strong data supporting intriguing emerging hypotheses about the mechanisms ..
  30. Analysis of metallo-beta-lactamase sequence constraints at high resolution
    Timothy Palzkill; Fiscal Year: 2013
    ..function will be determined by selecting functional clones from each library based on their ability to confer Escherichia coli resistance to a [unreadable]-lactam antibiotic of interest...
  31. Delivery of a bacterial inhibitor of NF-kB to colon tumors
    PHILIP ROSS HARDWIDGE; Fiscal Year: 2012
    ..This protein, NleH1, expressed by Escherichia coli O157:H7, binds to and inhibits the function of a newly identified subunit of NF-?B, the ribosomal protein S3 (..
  32. NOVEL SOLUBILITY-ENHANCING PROTEIN EXPRESSION TECHNOLOGY
    DAVID ALAN MEAD; Fiscal Year: 2013
    ..The most favored host for heterologous recombinant protein expression is Escherichia coli. Despite many improvements, producing soluble proteins in E...
  33. Phylogeny, Structure and Virulence Contributions of NMEC's Virulence Plasmids
    Lisa K Nolan; Fiscal Year: 2013
    ..neonatal bacterial meningitis is a major cause of mortality and morbidity worldwide with neonatal meningitis Escherichia coli (NMEC) being the most common agent of these infections...
  34. Maneuvering of Macrophage Function By Escherichia coli K1
    PRASADARAO V NEMANI; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Bacterial meningitis due to Escherichia coli K1 is a serious illness of the central nervous system. It is the most common pathogen causing meningitis in premature infants (46%)...
  35. Reciprocal Control of Motility and Adherence in UTI
    Harry L Mobley; Fiscal Year: 2013
    ..The most common uropathogen, Escherichia coli, can cause acute cystitis or pyelonephritis in the uncomplicated urinary tract...
  36. Discovery of Polymyxin-based Antibacterial Agents Active Against Multi-Drug Resis
    Lawrence H Lash; Fiscal Year: 2013
    ..In particular, the expansion of Gram-negative strains such as Klebsiella pneumonia, Escherichia coli, Acinetobacter baumannii and Pseudomonas aeruginosa and the rapidly spreading NDM-1 phenotypes are of grave ..
  37. Photoactivated Antimicrobial Collagen
    BARBARA ANN SOLTZ; Fiscal Year: 2013
    ..Similar results were obtained for Staphylococcus aureus, MRSA, Pseudomonas aeruginosa and Escherichia coli infections...
  38. National surveillance of emerging MDR in pediatric Enterobacteriaceae infections
    Danielle M Zerr; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: We propose to study antibiotic resistance in Enterobacteriaceae bacteria (such as Escherichia coli and Klebsiella pneumoniae) that produce urinary tract and bloodstream infections in children, by analyzing ..
  39. Challenges in beta-Lactamase Mediated Resistance
    Robert A Bonomo; Fiscal Year: 2013
    ..These [unreadable]-lactamases are found in ceftazidime resistant Klebsiella pneumoniae, Escherichia coli, Enterobacter spp., Acinetobacter baumannii, and Pseudomonas aeruginosa...
  40. Bordetella adenylate cyclase toxin: the role of cell interaction in toxin functio
    JOSHUA CLARK EBY; Fiscal Year: 2013
    ..of pore-forming bacterial protein toxins that are produced by other pathogenic bacteria such as uropathogenic Escherichia coli. Here, we will characterize the basic mechanisms of cell binding by AC toxin and show that these mechanisms ..
  41. Role of type IV secretion-mediated protein translocation in E. faecalis
    Peter J Christie; Fiscal Year: 2013
    ..faecalis donor cells to Escherichia coli recipients, and ii) a functional T4SS encoded by an 89-kilobase pathogenicity island (89K PAI) was shown to ..
  42. High-Throughput Assay Development for the Discovery of Small Molecule Inhibitors
    CHASE BEISEL; Fiscal Year: 2013
    ..Aim 1: Develop primary assays to screen for small molecule inhibitors of the Hfq-binding sRNA Spot 42 in Escherichia coli. The primary assay will rely on Spot 42, although the end goal is to identify inhibitors of global sRNA ..
  43. Characterization of a novel ETEC virulence regulator
    JOSEPH THOMAS WADE; Fiscal Year: 2012
    ..how transcription factors (TFs) regulate virulence processes in the pathogenic species, Enterotoxigenic Escherichia coli (ETEC)...
  44. REGULATION AND FUNCTION OF FIS IN E COLI
    Robert Osuna; Fiscal Year: 1999
    ..In Escherichia coli, the small DNA binding protein Fis is involved in an increasing number of cellular processes that include ..
  45. A New Point of Use Decontamination Method for Fresh Produce
    ADRIAN J DENVIR; Fiscal Year: 2010
    ..Foodborne illnesses in fruits and vegetables have been linked to several microbial species, including Escherichia coli O157:H7, Salmonella species, Listeria monocytogenes, Shigella species, Cyclospora cayetanensis, Hepatitis A ..
  46. Validating metabolic pathways in the intracellular pathogen Chlamydia trachomatis
    DEREK JAMES FISHER; Fiscal Year: 2010
    ..AroG, AroDE, and AroA) using genetic and biochemical approaches, including complementation studies in Escherichia coli null mutants, enzyme assays using purified recombinant proteins, and inhibitor assays;2) detecting chorismate ..
  47. Biological Effects of DNA Adducts Formed by Nitroaromatic Compounds
    ASHIS BASU; Fiscal Year: 2009
    ..to construct single-stranded plasmid or viral vectors and mutagenicity and genotoxicity will be evaluated in Escherichia coli and mammalian cells...
  48. Regulation of Streptomyces differentiation and antibiotic production by SoxR
    Monica Chander; Fiscal Year: 2010
    ..SoxR has traditionally been known as the mediator of an oxidative stress response in Escherichia coli and Salmonella enterica, but this role may be restricted to enteric bacteria...
  49. Novel Antibacterial Drugs Targeting DNA Repair Enzymes
    Gerald R Smith; Fiscal Year: 2010
    ..We will first screen for small-molecule inhibitors of RecBCD in Escherichia coli cells, using a simple, quick, and sensitive assay of nuclease activity in an easily grown E. coli strain...
  50. Protein Translocation by the Type III Secretion System
    Partho Ghosh; Fiscal Year: 2010
    ..Salmonella spp., and Escherichia coli O157:H7 among others...
  51. Development of the www.EcoliCommunity.org Information Resource
    Barry Wanner; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Basic knowledge of the biochemistry, molecular biology, and genetics of Escherichia coli K-12 may be more advanced than for any other organism...
  52. Bacterial heme transport and hemoglobin expression
    Douglas Henderson; Fiscal Year: 2009
    Our long-term goal is to produce large amounts of recombinant human hemoglobin in Escherichia coli to allow commercial production of the protein for formulation of hemoglobin-based oxygen carriers which will be used for blood ..
  53. Catalytic inactivation of miRNA function by customized RNase P-based ribozymes
    DANIEL R contact SCHOENBERG; Fiscal Year: 2010
    ..Our research design exploits the properties of M1 RNA, the catalytic subunit of Escherichia coli RNase P...
  54. Adherent Escherichia coli and associated virulence genes in children with IBD
    Sandra C Kim; Fiscal Year: 2010
    ..10 deficient (IL-10-/-) mice selectively colonized (monoassociated) with non-pathogenic strains of Escherichia coli (NC101) or Enterococcus faecalis (OG1RF) develop different phenotypes of colitis with clear regional and ..
  55. A new technology for preclinical development of functional bispecific antibodies
    Lee A Henderson; Fiscal Year: 2010
    ..potential of BsAbs has not been fully realized due to inefficient production methods, especially in Escherichia coli where several different BsAb formats have been met with limited success...
  56. MECHANISM AND CONTROL OF UREA BIOSYNTHESIS
    Frank M Raushel; Fiscal Year: 2011
    ..enzyme has recently been implicated in the biosynthesis of an extracellular death factor peptide, NNWNN, in Escherichia coli. The role of AsnA in the formation of this very unusual peptide will be determined...
  57. The novel cross-linking of the flagellar hook protein of Borrelia burgdorferi
    NYLES CHARON; Fiscal Year: 2012
    ..complexes will be purified from growing cells, and recombinant Bb FlgE (rFlgE) will be synthesized in Escherichia coli and purified as described...
  58. Limits and Trade-offs of Feedback Control
    Johan Paulsson; Fiscal Year: 2012
    ..design and interpret quantitative experiments for two systems that promote virulence and drug resistance in Escherichia coli. We focus on replication control of bacterial plasmids, where noise suppression is essential...
  59. Reactivity epitopes of ribonuclease III substrates
    ALLEN WHITALL NICHOLSON; Fiscal Year: 2010
    ..The mechanism of RNase III activation will be analyzed using an in vitro translation system. Along with Escherichia coli RNase III, this project will employ Aquifex aeolicus RNase III and Thermotoga maritima RNase III, for which ..
  60. MULTI-ANALYTE IMMUNOCHROMATOGRAPHIC TEST STRIP FOR BACTERIAL TOXIN DETECTION
    THOMAS W OWEN; Fiscal Year: 2011
    ..For example, Escherichia coli O157:H7 infections can spread through contaminated food, contaminated drinking water, contaminated swimming ..
  61. Exploiting deep ocean biology for biomedical discovery
    David C Rowley; Fiscal Year: 2011
    ..of both ATCC and clinical strains of multiple drug resistant Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae...
  62. Epidemiology of Community-Associated, ESBL-Producing Escherichia coli
    Yohei Doi; Fiscal Year: 2010
    Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has become widespread in healthcare settings. These organisms are not only resistant to most beta-lactams but also fluoroquinolones and aminoglycosides...
  63. Interaction between B. cereus RecQ helicase and topoisomerase III
    Zhiyu Li; Fiscal Year: 2010
    ..Unlike many single cellular organisms, such as Escherichia coli, Saccharomyces cerevisiae, and Saccharomyces pombe, which possess one or two type IA topoisomerases and ..
  64. Novel gene-silencing therapeutics for multidrug-resistant gram-negative pathogens
    DAVID ELIHU GREENBERG; Fiscal Year: 2013
    ..This group includes the Gram-negative pathogens Acinetobacter baumannii and Escherichia coli. A new paradigm in antibiotic discovery and design has recently been shown effective against numerous ..
  65. SHIGA-TOXINS: PRE-CLINICAL ANIMAL MODEL DEVELOPMENT AND THERAPEUTIC TESTING
    Shinichiro Kurosawa; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Shiga-like toxin producing enterohemorrhagic Escherichia coli (EHEC) strains are recognized as the most common cause of outbreaks and regional sporadic cases of bloody diarrhea and hemolytic uremic ..
  66. Short-term responses to temperature changes in Escherichia coli
    Christine A White Ziegler; Fiscal Year: 2010
    ..molecular biology approaches to explore the short-term response of both commensal and pathogenic strains of Escherichia coli to these temperatures...
  67. Pathogenic Mechanisms of Shiga Toxins
    Vernon L Tesh; Fiscal Year: 2010
    Infections with Shiga toxin (Stx)-producing bacteria Shigella dysenteriae 1 and Escherichia coli cause bloody diarrheal diseases which continue to be a major public health concern in developed and developing countries...
  68. Bacterial gene regulation by the NTP substrates of transcription initiation
    Charles L Turnbough; Fiscal Year: 2013
    ..The proposed studies will focus on Escherichia coli operons that encode proteins involved in pyrimidine biosynthesis or salvage...
  69. Electrochemical Method for Viable Pathogen Detection
    ADRIAN DENVIR; Fiscal Year: 2006
    ..is to develop a new, automated system for the rapid detection of bacterial contamination (especially Escherichia coli O157:H7) in meat and meat products...
  70. TREATMENT INTERVENTIONS FOR HEMORRHAGIC COLITIS
    Edgar Boedeker; Fiscal Year: 1999
    DESCRIPTION The broad aim of this application is to use a new animal model of enterohemorrhagic Escherichia coli (EHEC) infection to develop prophylactic and therapeutic regimens to prevent and treat EHEC disease...
  71. Molecular Modulation of Catalytic Sites of Escherichia coli F1Fo ATP Synthase
    Zulfiqar Ahmad; Fiscal Year: 2009
    ..0 million kg of ATP in a 75-year life span. In its simplest form, Escherichia coli contains eight different subunits. It can be separated into two sectors F1 and Fo...
  72. Mechanism of Regulating Division in Escherichia coli
    Jennifer R Juarez; Fiscal Year: 2012
    ..For this work the model organism Escherichia coli will be used...
  73. SHIGA-LIKE TOXIN OF ESCHERICHIA COLI
    ALISON O BRIEN; Fiscal Year: 1991
    b>Escherichia coli can cause diarrhea by several distinct mechanisms. A newly recognized group of E. coli cause hemorrhagic colitis and the hemolytic uremic syndrome. These enterohemorrhagic E...
  74. ESCHERICHIA COLI LT-RELATED ENTEROTOXINS
    RICHARD FINKELSTEIN; Fiscal Year: 1991
    ..k.a. LTs) from diarrheagenic strains of Escherichia coli of human (H) origin; LT from strains of E. coli of porcine (P) origin; a distinct LT, LT-II, from an E...
  75. REGULATION BY THE ESCHERICHIA COLI SMALL RNA, DSRA
    DARREN SLEDJESKI; Fiscal Year: 2002
    DsrA is a small, stable, untranslated RNA that is conserved between Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium. DsrA RNA regulates two different global regulatory networks...
  76. Determining vaccine candidacy of LT and STa toxoid fusions against enterotoxigeni
    Weiping Zhang; Fiscal Year: 2010
    Enterotoxigenic Escherichia coli (ETEC) strains are categorized as priority B pathogens by NIAID...
  77. Glycosylation of full-length antibodies in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    ..Full-length monoclonal antibodies (immunoglobulins) can be produced in Escherichia coli that are functionally similar to those produced in mammalian cells except in the ability to bind macrophage ..
  78. Humanizing N-linked glycosylation in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    ..jejuni asparagine-linked (Nlinked) protein glycosylation system can be functionally transferred into Escherichia coli, giving the recipient E. coli cells the ability to glycosylate proteins...
  79. Pathogenicity of Shiga Toxin-Producing E. coli
    ALISON O BRIEN; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): Enterohemorrhagic Escherichia coli (EHEC) O157:H7 belongs to a subset of Shiga toxin-producing E coli (STEC) that makes Shiga toxin (Stx) type 1 or type 2 (or a variant thereof), or both toxins, ..
  80. DNA METHYLATION AND PILI GENE REGULATION
    David Low; Fiscal Year: 2006
    ..and PapI orchestrate the reversible switch between OFF and ON Pap pili expression states in uropathogenic Escherichia coli (UPEC)...
  81. MECHANISM OF CARBOHYDRATE TRANSPORT IN BACTERIA
    Milton Saier; Fiscal Year: 2000
    Enteric bacteria such as Escherichia coli and Salmonella species are principal causative agents of diarrhea, dysentery, urinary tract infections, meningitis in children, septicemia, enteric fevers, an pneumonia...
  82. GENETIC ANALYSIS TO THE SIGNAL RECOGNITION PARTICLE
    Gregory Phillips; Fiscal Year: 1999
    ..to components of the mammalian SRP and SRP receptor have recently been found throughout nature, including in Escherichia coli. E. coli homologues include products of the ffh, ffs, and ftsY genes. All of these genes are essential for E...
  83. E COLI TRANSLOCATION ACROSS INTESTINAL EPITHELIUM
    Michelle Pietzak; Fiscal Year: 2003
    ..D., Dr. Pietzak will continue to pursue her basic investigations into the mechanisms by which Escherichia coli (E. coli) is able to translocate across intestinal epithelium and cause sepsis. E...
  84. THE CLONAL NATURE OF CYTOTOXIGENIC E. COLI
    THOMAS WHITTAM; Fiscal Year: 1992
    ..research is to define the clonal population structure and genetic relationships of pathogenic forms of Escherichia coli, with special reference to cytotoxin-producing strains that only recently have been linked to enteric disease ..
  85. THE CLONAL NATURE OF CYTOTOXIGENIC E. COLI
    THOMAS WHITTAM; Fiscal Year: 1991
    ..research is to define the clonal population structure and genetic relationships of pathogenic forms of Escherichia coli, with special reference to cytotoxin-producing strains that only recently have been linked to enteric disease ..
  86. VIRUL FACTORS OF UROPATH ESCHERICHIA COLI
    Peter Redford; Fiscal Year: 2003
    b>Escherichia coli is the most common cause of community-acquired urinary tract infections, resulting in an estimated eight million physician visits a year in the United States. It is also a leading cause of nosocomial infections...