Enterobacter sp. 638

Summary

Alias:

Top Publications

  1. ncbi The UDP-N-acetylglucosamine 1-carboxyvinyl-transferase of Enterobacter cloacae. Molecular cloning, sequencing of the gene and overexpression of the enzyme
    C Wanke
    Institut für Pflanzenwissenschaften, Eidgenossische Technische Hochschule, Zurich, Switzerland
    FEBS Lett 301:271-6. 1992
  2. ncbi Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase. Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181, His184
    Huma Khan
    Department of Biochemistry, University of Leicester, UK
    FEBS J 272:4660-71. 2005
  3. ncbi A novel inhibitor that suspends the induced fit mechanism of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA)
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 280:14070-5. 2005
  4. ncbi Evidence that the fosfomycin target Cys115 in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product release
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 280:3757-63. 2005
  5. ncbi Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation
    Huma Khan
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 279:30563-72. 2004
  6. ncbi A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate states
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 278:49215-22. 2003
  7. ncbi Kinetic and structural basis of reactivity of pentaerythritol tetranitrate reductase with NADPH, 2-cyclohexenone, nitroesters, and nitroaromatic explosives
    Huma Khan
    Department of Biochemistry and Centre for Chemical Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 277:21906-12. 2002
  8. ncbi Structures of nitroreductase in three states: effects of inhibitor binding and reduction
    Chad A Haynes
    Department of Molecular and Cellular Biochemistry, The University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 277:11513-20. 2002
  9. ncbi Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme
    T M Barna
    Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, UK
    J Mol Biol 310:433-47. 2001
  10. ncbi Comparative X-ray analysis of the un-liganded fosfomycin-target murA
    S Eschenburg
    Max Planck Institute for Medical Research, Department of Biophysics, Heidelberg, Germany
    Proteins 40:290-8. 2000

Research Grants

  1. Characterization of novel MurA inhibitors
    Ernst Schonbrunn; Fiscal Year: 2006

Scientific Experts

  • P Kuhnert
  • E Schonbrunn
  • Susanne Eschenburg
  • Huma Khan
  • Neil C Bruce
  • Terez Barna
  • Nigel S Scrutton
  • Andrew W Munro
  • Peter C E Moody
  • Richard J Harris
  • Chad A Haynes
  • T M Barna
  • S Eschenburg
  • David Leys
  • Melanie A Priestman
  • Carole Delachaume
  • Melanie Priestman
  • Florence Fassy
  • Farid A Abdul-Latif
  • Igor Barsukov
  • Wolfgang Kabsch
  • Martha L Healy
  • David W Rodgers
  • Ronald L Koder
  • Anne Frances Miller
  • S Sack
  • Daniel H Craig
  • N S Scrutton
  • I Barsukov
  • N C Bruce
  • H Khan
  • P C Moody
  • C Wanke
  • N Amrhein
  • Z Dauter
  • E Mandelkow
  • R Falchetto
  • C Bryant
  • M DeLuca

Detail Information

Publications16

  1. ncbi The UDP-N-acetylglucosamine 1-carboxyvinyl-transferase of Enterobacter cloacae. Molecular cloning, sequencing of the gene and overexpression of the enzyme
    C Wanke
    Institut für Pflanzenwissenschaften, Eidgenossische Technische Hochschule, Zurich, Switzerland
    FEBS Lett 301:271-6. 1992
    ....
  2. ncbi Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase. Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181, His184
    Huma Khan
    Department of Biochemistry, University of Leicester, UK
    FEBS J 272:4660-71. 2005
    ..Our data highlight mechanistic differences between Old Yellow Enzyme and PETN reductase and indicate that catalysis requires a metastable enzyme-steroid complex and not the most stable complex observed in crystallographic studies...
  3. ncbi A novel inhibitor that suspends the induced fit mechanism of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA)
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 280:14070-5. 2005
    ..The results provide evidence for the existence of a MurA.UNAG collision complex that may be specifically targeted by small molecules different from ground-state analogs of the enzymatic reaction...
  4. ncbi Evidence that the fosfomycin target Cys115 in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product release
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 280:3757-63. 2005
    ..Phosphate departure appears to trigger a suite of conformational changes, which finally leads to opening of the two-domain structure of MurA and the release of the second product enolpyruvyl-UDP-N-acetylglucosamine...
  5. ncbi Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation
    Huma Khan
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 279:30563-72. 2004
    ..Implications for the mechanism of high explosive degradation by PETN reductase are discussed...
  6. ncbi A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate states
    Susanne Eschenburg
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 278:49215-22. 2003
    ..The presented structures lead to a new view of the catalytic mechanism and, moreover, provide an ideal starting point for the rational design of potent inhibitors of MurA and AroA...
  7. ncbi Kinetic and structural basis of reactivity of pentaerythritol tetranitrate reductase with NADPH, 2-cyclohexenone, nitroesters, and nitroaromatic explosives
    Huma Khan
    Department of Biochemistry and Centre for Chemical Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    J Biol Chem 277:21906-12. 2002
    ..Our studies provide a structural and mechanistic rationale for the ability of PETN reductase to react with the nitroaromatic explosive compounds TNT and picric acid and for the inhibition of enzyme activity with 2,4-DNP...
  8. ncbi Structures of nitroreductase in three states: effects of inhibitor binding and reduction
    Chad A Haynes
    Department of Molecular and Cellular Biochemistry, The University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 277:11513-20. 2002
    ..Comparison of the two inhibitor complexes shows that a portion of helix H6 can flex to accommodate the differently sized inhibitors suggesting a mechanism for accommodating varied substrates...
  9. ncbi Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme
    T M Barna
    Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, UK
    J Mol Biol 310:433-47. 2001
    ..These studies rule out lateral motion of the steroid and indicate that the steroid orientation is "flipped" in different redox states of the enzyme...
  10. ncbi Comparative X-ray analysis of the un-liganded fosfomycin-target murA
    S Eschenburg
    Max Planck Institute for Medical Research, Department of Biophysics, Heidelberg, Germany
    Proteins 40:290-8. 2000
    ..The homogeneous population with L-isoaspartate in both structures suggests that the modification in Enterobacter cloacae MurA is not a mere aging defect but rather the result of a specific in vivo process...
  11. pmc Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA
    E Schonbrunn
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 97:6345-9. 2000
    ..Substrate binding to MurA is accompanied by large movements especially of the loop and Arg-91, which explains why ANS is an excellent sensor of conformational changes during catalysis of this pharmaceutically important enzyme...
  12. ncbi Role of the loop containing residue 115 in the induced-fit mechanism of the bacterial cell wall biosynthetic enzyme MurA
    E Schonbrunn
    Swiss Federal Institute of Technology, Institute of Plant Sciences, Universitatstrasse 2, CH 8092 Zurich, Switzerland
    Biochemistry 39:2164-73. 2000
    ....
  13. ncbi Crystal structure of UDP-N-acetylglucosamine enolpyruvyltransferase, the target of the antibiotic fosfomycin
    E Schonbrunn
    Max Planck Unit for Structural Molecular Biology, Notkestr 85, c o DESY, D 22603 Hamburg, Germany
    Structure 4:1065-75. 1996
    ..EPT is of potential pharmaceutical interest because it is inhibited by the broad spectrum antibiotic fosfomycin...
  14. ncbi Crystallization and preliminary X-ray diffraction analysis of UDP-N-acetylglucosamine enolpyruvyltransferase of Enterobacter cloacae
    S Sack
    Max Planck Unit for Structural Molecular Biology, Hamburg, Germany
    J Struct Biol 117:73-6. 1996
    ..9 A, b = 155.9 A, c = 83.8 A, beta = 91.6 degrees. Assuming two monomers per asymmetric unit, the solvent content of these crystals is 63%. Flash-frozen crystals diffract to beyond 2 A resolution...
  15. ncbi Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae
    C Bryant
    Department of Chemistry, University of California, San Diego, La Jolla 92093
    J Biol Chem 266:4119-25. 1991
    ....
  16. doi Phylogeny and prediction of genetic similarity of Cronobacter and related taxa by multilocus sequence analysis (MLSA)
    Peter Kuhnert
    Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, CH 3001 Bern, Switzerland
    Int J Food Microbiol 136:152-8. 2009
    ....

Research Grants1

  1. Characterization of novel MurA inhibitors
    Ernst Schonbrunn; Fiscal Year: 2006
    ..The long-term goal of this proposal is to provide a basis for the rational design of novel potent broad-spectrum antibacterial drugs that specifically target MurA. ..