Coxiella burnetii RSA 493


Alias: Coxiella burnetii str. RSA 493

Top Publications

  1. Beare P, Gilk S, Larson C, Hill J, Stead C, Omsland A, et al. Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages. MBio. 2011;2:e00175-11 pubmed publisher
  2. Lin Z, Mallavia L. The partition region of plasmid QpH1 is a member of a family of two trans-acting factors as implied by sequence analysis. Gene. 1995;160:69-74 pubmed
    ..Despite lower homologies, both QsopA and QsopB did share conserved aa sequence regions and invariant residues with other members within each family. ..
  3. Mo Y, Mallavia L. A Coxiella burnetii gene encodes a sensor-like protein. Gene. 1994;151:185-90 pubmed
  4. Wang X, Xiong X, Graves S, Stenos J, Wen B. Protein array of Coxiella burnetii probed with Q fever sera. Sci China Life Sci. 2013;56:453-9 pubmed publisher
    ..These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever. ..
  5. Heinzen R, Hackstadt T. A developmental stage-specific histone H1 homolog of Coxiella burnetii. J Bacteriol. 1996;178:5049-52 pubmed
    ..C. burnetii appears to be another example of an intracellular parasite with morphologically distinct developmental forms whose nucleoid structure may be controlled by histone H1 homologs. ..
  6. Willems H, Thiele D, Krauss H. Sequencing and linkage analysis of a Coxiella burnetii 2.1 kb NotI fragment. Eur J Epidemiol. 1995;11:559-61 pubmed
    ..Upon completing the ORF for the hemA gene by sequencing the adjacent NotI/EcoRI fragment a third ORF for the putative prfA gene was determined coding for release factor 1, an enzyme catalysing the last step in protein biosynthesis. ..
  7. Oswald W, Thiele D. A sporulation gene in Coxiella burnetii?. Zentralbl Veterinarmed B. 1993;40:366-70 pubmed
    ..This is first evidence of the molecular basis for formation of spores in Coxiella burnetii which has been postulated in the literature by electron microscopic investigations. ..
  8. Heinzen R, Frazier M, Mallavia L. Coxiella burnetii superoxide dismutase gene: cloning, sequencing, and expression in Escherichia coli. Infect Immun. 1992;60:3814-23 pubmed
    ..Isolation of the C. burnetii SOD gene may provide an opportunity to examine its role in the intracellular survival of this rickettsia. ..
  9. Luedtke B, Mahapatra S, Lutter E, Shaw E. The Coxiella Burnetii type IVB secretion system (T4BSS) component DotA is released/secreted during infection of host cells and during in vitro growth in a T4BSS-dependent manner. Pathog Dis. 2017;75: pubmed publisher
    ..These data are the first to identify the release/secretion of C. burnetii T4BSS components during axenic growth and the interaction of a T4BSS component with the PV membrane during infection of host cells. ..

More Information


  1. Zuber M, Hoover T, Court D. Cloning, sequencing and expression of the dnaJ gene of Coxiella burnetii. Gene. 1995;152:99-102 pubmed
    ..coli DnaJ protein. Deletion mapping and genetic complementation demonstrated that C. burnetii dnaJ is present on a 2-kb EcoRI-HindIII genomic DNA fragment, from which the nt sequence of the C. burnetii dnaJ gene was determined. ..
  2. Lin Z, Howe D, Mallavia L. Roa307, a protein encoded on Coxiella burnetii plasmid QpH1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes. Mol Gen Genet. 1995;248:487-90 pubmed
    ..Expression of roa307 was demonstrated by PhoA activity of an Roa307-PhoA fusion. ..
  3. Heinzen R, Mo Y, Robertson S, Mallavia L. Characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia Coxiella burnetii. Gene. 1995;155:27-34 pubmed
    ..coli wild type. A plasmid clone lacking the sdh upstream regulatory region did not complement nor produce sdh mRNA by dot blot analysis.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  4. Battisti J, Hicks L, Minnick M. A unique Coxiella burnetii lipoprotein involved in metal binding (LimB). Microbiology. 2011;157:966-76 pubmed publisher
    ..Overall, the results suggest that LimB is a unique Coxiella lipoprotein that serves as a surface receptor for divalent metal cations and may play a role in acquiring at least one of these metals during intracellular growth. ..
  5. Minnick M, Small C, Frazier M, Mallavia L. Analysis of the cbhE' plasmid gene from acute disease-causing isolates of Coxiella burnetii. Gene. 1991;103:113-8 pubmed
    ..CbhE is predominantly hydrophilic with a predicted pI of 4.43. The function of CbhE is unknown. No nt or aa sequences with homology to cbhE' or CbhE, respectively, were found in searches of a number of data bases. ..
  6. Heinzen R, Frazier M, Mallavia L. Sequence and linkage analysis of the Coxiella burnetii citrate synthase-encoding gene. Gene. 1991;109:63-9 pubmed
    ..coli succinate dehydrogenase. This report represents the first nt sequence analysis of a gene of known function from the obligate intracellular parasite, C. burnetii. ..
  7. Light S, Krishna S, Minasov G, Anderson W. An Unusual Cation-Binding Site and Distinct Domain-Domain Interactions Distinguish Class II Enolpyruvylshikimate-3-phosphate Synthases. Biochemistry. 2016;55:1239-45 pubmed publisher
    ..Finally, we identify distinct patterns of sequence conservation at the domain-domain interface and propose that the two EPSPS classes have evolved to differently optimize domain opening-closing dynamics. ..
  8. Zuber M, Hoover T, Court D. Analysis of a Coxiella burnetti gene product that activates capsule synthesis in Escherichia coli: requirement for the heat shock chaperone DnaK and the two-component regulator RcsC. J Bacteriol. 1995;177:4238-44 pubmed
    ..Discussions are presented that suggest three different roles for alternative forms of the DnaK-chaperone machinery in capsule production. ..
  9. Fielden L, Moffatt J, Kang Y, Baker M, Khoo C, Roy C, et al. A Farnesylated Coxiella burnetii Effector Forms a Multimeric Complex at the Mitochondrial Outer Membrane during Infection. Infect Immun. 2017;85: pubmed publisher
    ..This research reveals that mitochondria are a bona fide target of Coxiella effectors and MceA is a complex-forming effector at the mitochondrial outer membrane during Coxiella infection. ..
  10. Lin Z, Mallavia L. Identification of a partition region carried by the plasmid QpH1 of Coxiella burnetii. Mol Microbiol. 1994;13:513-23 pubmed
    ..These data suggest that fragment C carries the plasmid partition region of the plasmid QpH1. ..
  11. Zuber M, Hoover T, Powell B, Court D. Analysis of the rnc locus of Coxiella burnetii. Mol Microbiol. 1994;14:291-300 pubmed
    ..coli of three proteins with approximate molecular masses of 35, 27 and 25 kDa. Antibodies against purified E. coli Era protein cross-reacted with the 35 kDa protein of C. burnetii on Western blots. ..
  12. Weber M, Faris R, McLachlan J, Tellez A, Wright W, Galvan G, et al. Modulation of the host transcriptome by Coxiella burnetii nuclear effector Cbu1314. Microbes Infect. 2016;18:336-45 pubmed publisher
    ..Collectively these results suggest that Cbu1314 associates with host chromatin and plays a role in modulating the host transcriptome. ..
  13. Seshadri R, Hendrix L, Samuel J. Differential expression of translational elements by life cycle variants of Coxiella burnetii. Infect Immun. 1999;67:6026-33 pubmed
    ..Identification of two proteins differentially expressed by C. burnetii, EF-Tu and EF-Ts, both essential components of the translational machinery of the cell, supports the hypothesis that LCVs are metabolically more active than SCVs...
  14. Gilk S, Beare P, Heinzen R. Coxiella burnetii expresses a functional ?24 sterol reductase. J Bacteriol. 2010;192:6154-9 pubmed publisher
    ..These data indicate CBU1206 is an active sterol reductase and suggest the enzyme may act on host sterols during C. burnetii intracellular growth...
  15. Raghavan R, Miller S, Hicks L, Minnick M. The unusual 23S rRNA gene of Coxiella burnetii: two self-splicing group I introns flank a 34-base-pair exon, and one element lacks the canonical omegaG. J Bacteriol. 2007;189:6572-9 pubmed publisher
    ..burnetii's 23S rRNA gene is very unusual for an obligate intracellular bacterium and suggests a recent shift to its current lifestyle from a previous niche with greater opportunities for lateral gene transfer...
  16. Li Q, Niu D, Wen B, Chen M, Qiu L, Zhang J. Protective immunity against Q fever induced with a recombinant P1 antigen fused with HspB of Coxiella burnetii. Ann N Y Acad Sci. 2005;1063:130-42 pubmed publisher
    ..The data from this study indicate that fusion antigen P1-HspB is a good immunogen for eliciting immunoresponses against C. burnetii, and it may be a more suitable candidate for preparing subunit vaccine against Q fever...
  17. Varghees S, Kiss K, Frans G, Braha O, Samuel J. Cloning and porin activity of the major outer membrane protein P1 from Coxiella burnetii. Infect Immun. 2002;70:6741-50 pubmed
    ..Characterization of differentially expressed P1 as a porin increases our understanding of the function of morphological variants and their role in pathogenesis...
  18. Hoover T, Culp D, Vodkin M, Williams J, Thompson H. Chromosomal DNA deletions explain phenotypic characteristics of two antigenic variants, phase II and RSA 514 (crazy), of the Coxiella burnetii nine mile strain. Infect Immun. 2002;70:6726-33 pubmed
    ..Repeats, redundancies, paralogous genes, and two regions with reduced G+C contents were found within the deletions...
  19. Seshadri R, Samuel J. Characterization of a stress-induced alternate sigma factor, RpoS, of Coxiella burnetii and its expression during the development cycle. Infect Immun. 2001;69:4874-83 pubmed publisher
    ..These results suggest that LCV and SCV are not comparable to logarithmic and stationary phases of growth and may represent a novel adaptation for survival in both the phagolysosome and the extracellular environment...
  20. Nguyen S, Hirai K. Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol Lett. 1999;180:249-54 pubmed
    ..Taken together, the study results suggest that the icd-based differentiation method may be useful in clinical investigation of Coxiella infections...
  21. Van Nguyen S, To H, Yamaguchi T, Fukushi H, Hirai K. Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii. FEMS Microbiol Lett. 1999;175:101-6 pubmed
    ..This distinct property of the cloned IDH suggests that C. burnetii icd gene may have a role in the adaptation of the organism to the harsh acidic environment of the eucaryotic phagolysosomes...
  22. Macellaro A, Tujulin E, Hjalmarsson K, Norlander L. Identification of a 71-kilodalton surface-associated Hsp70 homologue in Coxiella burnetii. Infect Immun. 1998;66:5882-8 pubmed
    ..The dual localization was verified by Western blot analysis of proteins in C. burnetii cell fractions, using purified antibodies directed to the 71-kDa protein...
  23. Willems H, J ger C, Baljer G. Physical and genetic map of the obligate intracellular bacterium Coxiella burnetii. J Bacteriol. 1998;180:3816-22 pubmed
    ..The genetic map will help to determine whether gene order in different isolates is conserved...
  24. Mollet C, Drancourt M, Raoult D. Determination of Coxiella burnetii rpoB sequence and its use for phylogenetic analysis. Gene. 1998;207:97-103 pubmed
    ..burnetii belongs to the gamma-group of Proteobacteria. Furthermore, phylogeny inferred from comparison of RpoB, or homologous sequences including Archae, Bacteria and Eukarya, concurred with these results...
  25. Heinzen R, Frazier M, Mallavia L. Nucleotide sequence of Coxiella burnetii superoxide dismutase. Nucleic Acids Res. 1990;18:6437 pubmed
  26. Vodkin M, Williams J. A heat shock operon in Coxiella burnetti produces a major antigen homologous to a protein in both mycobacteria and Escherichia coli. J Bacteriol. 1988;170:1227-34 pubmed
    ..Since the recombinant protein was immunogenic, it may serve as an efficacious vaccine against C. burnetii and other pathogenic microorganisms that express the conserved antigen...
  27. Mo Y, Cianciotto N, Mallavia L. Molecular cloning of a Coxiella burnetii gene encoding a macrophage infectivity potentiator (Mip) analogue. Microbiology. 1995;141 ( Pt 11):2861-71 pubmed publisher
    ..Furthermore, TnphoA mutagenesis demonstrated that in E. coli CbMip was secreted. In view of the role of Mip/Mip-like proteins in the pathogenesis of Legionella and Chlamydia, CbMip may be a C. burnetii virulence factor...
  28. Suhan M, Chen S, Thompson H, Hoover T, Hill A, Williams J. Cloning and characterization of an autonomous replication sequence from Coxiella burnetii. J Bacteriol. 1994;176:5233-43 pubmed
    ..Two-dimensional agarose gel electrophoresis did not reveal the presence of initiating intermediates, indicating that the ARS did not initiate chromosome replication during laboratory growth of C. burnetii...
  29. Afseth G, Mo Y, Mallavia L. Characterization of the 23S and 5S rRNA genes of Coxiella burnetii and identification of an intervening sequence within the 23S rRNA gene. J Bacteriol. 1995;177:2946-9 pubmed
    ..An open reading frame in the IVS has been identified that shows 70% similarity at the amino acid level to IVS open reading frames characterized from four species of Leptospira...
  30. Martinez E, Cantet F, Fava L, Norville I, Bonazzi M. Identification of OmpA, a Coxiella burnetii protein involved in host cell invasion, by multi-phenotypic high-content screening. PLoS Pathog. 2014;10:e1004013 pubmed publisher
    ..In summary, we have developed multi-phenotypic assays for the study of host/pathogen interactions. By applying our methods to Coxiella burnetii, we have identified the first Coxiella protein involved in host cell invasion...
  31. Hendrix L, Mallavia L, Samuel J. Cloning and sequencing of Coxiella burnetii outer membrane protein gene com1. Infect Immun. 1993;61:470-7 pubmed
  32. Hoover T, Vodkin M, Williams J. A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence. J Bacteriol. 1992;174:5540-8 pubmed
    ..Despite the sequence changes, all three copies of the element have retained significant dyad symmetry in this region...
  33. Willems H, Ritter M, J ger C, Thiele D. Plasmid-homologous sequences in the chromosome of plasmidless Coxiella burnetii Scurry Q217. J Bacteriol. 1997;179:3293-7 pubmed
    ..Compared to plasmid QpH1, 6 ORFs were identical, 5 ORFs were different in size, 6 ORFs were newly generated, and 25 ORFs were lost. It was found that plasmid-homologous sequences in Scurry Q217 were of chromosomal origin...