fission yeast


Alias: Schizosaccharomyces pombe, Schizosaccharomyces malidevorans, Schizosaccharomyces pombeP

Top Publications

  1. Mikheyeva I, Grady P, Tamburini F, Lorenz D, Cam H. Multifaceted genome control by Set1 Dependent and Independent of H3K4 methylation and the Set1C/COMPASS complex. PLoS Genet. 2014;10:e1004740 pubmed publisher
    ..Here, we show that the fission yeast Schizosaccharomyces pombe Set1 utilizes distinct domain modules to regulate disparate classes of repetitive elements associated ..
  2. Tada K, Susumu H, Sakuno T, Watanabe Y. Condensin association with histone H2A shapes mitotic chromosomes. Nature. 2011;474:477-83 pubmed publisher
    ..Here we show that fission yeast (Schizosaccharomyces pombe) kinetochore proteins Pcs1 and Mde4--homologues of budding yeast (Saccharomyces cerevisiae) monopolin ..
  3. Zich J, Sochaj A, Syred H, Milne L, Cook A, Ohkura H, et al. Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex. Curr Biol. 2012;22:296-301 pubmed publisher
    ..Here we demonstrate that a kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and that levels of APC/C-associated Mad2 and Mad3 are dramatically ..
  4. Shimanuki M, Uehara L, Pluskal T, Yoshida T, Kokubu A, Kawasaki Y, et al. Klf1, a C2H2 zinc finger-transcription factor, is required for cell wall maintenance during long-term quiescence in differentiated G0 phase. PLoS ONE. 2013;8:e78545 pubmed publisher
    b>Fission yeast, Schizoaccharomyces pombe, is a model for studying cellular quiescence. Shifting to a medium that lacks a nitrogen-source induces proliferative cells to enter long-term G0 quiescence...
  5. Lando D, Endesfelder U, Berger H, Subramanian L, Dunne P, McColl J, et al. Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast. Open Biol. 2012;2:120078 pubmed publisher
    ..centromere-specific histone H3 variant CENP-A(Cnp1) with single-molecule sensitivity in fission yeast (Schizosaccharomyces pombe). The results obtained are validated by and compared with ChIP-seq analyses...
  6. Moss J, Tinline Purvis H, Walker C, Folkes L, Stratford M, Hayles J, et al. Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast. Genes Dev. 2010;24:2705-16 pubmed publisher
    ..we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonucleotide reductase ..
  7. Tumbale P, Appel C, Kraehenbuehl R, Robertson P, Williams J, Krahn J, et al. Structure of an aprataxin-DNA complex with insights into AOA1 neurodegenerative disease. Nat Struct Mol Biol. 2011;18:1189-95 pubmed publisher
    ..Here the structure of a Schizosaccharomyces pombe Aptx-DNA-AMP-Zn(2+) complex reveals active site and DNA interaction clefts formed by fusing a histidine ..
  8. Atkin J, Hálová L, Ferguson J, Hitchin J, Lichawska Cieslar A, Jordan A, et al. Torin1-mediated TOR kinase inhibition reduces Wee1 levels and advances mitotic commitment in fission yeast and HeLa cells. J Cell Sci. 2014;127:1346-56 pubmed publisher
    ..the catalytic site of TOR with the Torin1 inhibitor completely arrests growth without cell death in Schizosaccharomyces pombe. A mutation of the Tor2 glycine residue (G2040D) that lies adjacent to the key Torin-interacting ..
  9. Tange Y, Kurabayashi A, Goto B, Hoe K, Kim D, Park H, et al. The CCR4-NOT complex is implicated in the viability of aneuploid yeasts. PLoS Genet. 2012;8:e1002776 pubmed publisher
    ..sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell...

More Information

Publications307 found, 100 shown here

  1. Yamanaka S, Mehta S, REYES TURCU F, Zhuang F, Fuchs R, Rong Y, et al. RNAi triggered by specialized machinery silences developmental genes and retrotransposons. Nature. 2013;493:557-60 pubmed publisher
    ..Here we show that, in the fission yeast Schizosaccharomyces pombe, RNAi and heterochromatin factors cooperate to silence diverse loci, including sexual differentiation ..
  2. Schiller C, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, et al. Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling. Nat Struct Mol Biol. 2012;19:693-700 pubmed publisher
    ..To understand the functional architecture of MRN, we determined the crystal structures of the Schizosaccharomyces pombe Mre11 dimeric catalytic domain alone and in complex with a fragment of Nbs1...
  3. Navarro F, Nurse P. A systematic screen reveals new elements acting at the G2/M cell cycle control. Genome Biol. 2012;13:R36 pubmed publisher
    ..In the fission yeast Schizosaccharomyces pombe the activation of the G2/M CDK is regulated primarily by dephosphorylation of the conserved residue ..
  4. Aygün O, Mehta S, Grewal S. HDAC-mediated suppression of histone turnover promotes epigenetic stability of heterochromatin. Nat Struct Mol Biol. 2013;20:547-54 pubmed publisher
    ..HDAC) Clr3 as a factor required for inhibiting histone turnover across heterochromatin domains in Schizosaccharomyces pombe. Loss of RNA-interference factors, Clr4 methyltransferase or HP1 proteins involved in HDAC localization ..
  5. Castillo A, Pidoux A, Catania S, Durand Dubief M, Choi E, Hamilton G, et al. Telomeric repeats facilitate CENP-A(Cnp1) incorporation via telomere binding proteins. PLoS ONE. 2013;8:e69673 pubmed publisher
    ..deposition we investigated whether certain locations are favoured when additional CENP-A(Cnp1) is present in fission yeast cells...
  6. Buscaino A, White S, Houston D, Lejeune E, Simmer F, de Lima Alves F, et al. Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. PLoS Genet. 2012;8:e1002499 pubmed publisher
    Non-coding transcription can trigger histone post-translational modifications forming specialized chromatin. In fission yeast, heterochromatin formation requires RNAi and the histone H3K9 methyltransferase complex CLRC, composed of Clr4, ..
  7. Tashiro S, Asano T, Kanoh J, Ishikawa F. Transcription-induced chromatin association of RNA surveillance factors mediates facultative heterochromatin formation in fission yeast. Genes Cells. 2013;18:327-39 pubmed publisher
    ..Here, we show that two meiotic gene loci in fission yeast, mei4 and ssm4, comprise facultative heterochromatin that is regulated in a developmental stage-dependent ..
  8. Chen Y, Rai R, Zhou Z, Kanoh J, Ribeyre C, Yang Y, et al. A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms. Nat Struct Mol Biol. 2011;18:213-21 pubmed publisher
    ..It is involved in protecting chromosome ends in fission yeast and promoting gene silencing in Saccharomyces cerevisiae, whereas it represses homology-directed recombination ..
  9. Taoka M, Ishikawa D, Nobe Y, Ishikawa H, Yamauchi Y, Terukina G, et al. RNA cytidine acetyltransferase of small-subunit ribosomal RNA: identification of acetylation sites and the responsible acetyltransferase in fission yeast, Schizosaccharomyces pombe. PLoS ONE. 2014;9:e112156 pubmed publisher
    ..We performed a comprehensive MS-based analysis covering the entire sequence of the fission yeast, Schizosaccharomyces pombe, SSU rRNA and identified two acetylcytidines at positions 1297 and 1815 in the 3' half of the rRNA...
  10. Yamane K, Mizuguchi T, Cui B, Zofall M, Noma K, Grewal S. Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci. Mol Cell. 2011;41:56-66 pubmed publisher
    ..In fission yeast, HP1 proteins Chp2 and Swi6, which bind to methylated histone H3 lysine 9, associate with SHREC (Snf2/HDAC ..
  11. Buscaino A, Lejeune E, Audergon P, Hamilton G, Pidoux A, Allshire R. Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance. EMBO J. 2013;32:1250-64 pubmed publisher
    ..Here, we investigate the requirements for establishment, spreading and maintenance of heterochromatin using fission yeast centromeres as a paradigm...
  12. Kelly F, Nurse P. Spatial control of Cdc42 activation determines cell width in fission yeast. Mol Biol Cell. 2011;22:3801-11 pubmed publisher
    The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that grows by linear extension at the cell tips, with a nearly constant width throughout the cell cycle...
  13. Lorenz A, Osman F, Sun W, Nandi S, Steinacher R, Whitby M. The fission yeast FANCM ortholog directs non-crossover recombination during meiosis. Science. 2012;336:1585-8 pubmed publisher
    ..We show that Fml1, the Fanconi anemia complementation group M (FANCM)-ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway...
  14. Bajpai A, Feoktistova A, Chen J, McCollum D, Sato M, Carazo Salas R, et al. Dynamics of SIN asymmetry establishment. PLoS Comput Biol. 2013;9:e1003147 pubmed publisher
    Timing of cell division is coordinated by the Septation Initiation Network (SIN) in fission yeast. SIN activation is initiated at the two spindle pole bodies (SPB) of the cell in metaphase, but only one of these SPBs contains an active ..
  15. REYES TURCU F, Zhang K, Zofall M, Chen E, Grewal S. Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin. Nat Struct Mol Biol. 2011;18:1132-8 pubmed publisher
    Heterochromatin assembly at Schizosaccharomyces pombe centromeres involves a self-reinforcing loop mechanism wherein chromatin-bound RNAi factors facilitate targeting of Clr4-Rik1 methyltransferase...
  16. Arasada R, Pollard T. Contractile ring stability in S. pombe depends on F-BAR protein Cdc15p and Bgs1p transport from the Golgi complex. Cell Rep. 2014;8:1533-44 pubmed publisher
    Cdc15p is known to contribute to cytokinesis in fission yeast; however, the protein is not required to assemble the contractile ring of actin and myosin, but it helps to anchor the ring to the plasma membrane...
  17. Wood E, Nurse P. Pom1 and cell size homeostasis in fission yeast. Cell Cycle. 2013;12:3228-36 pubmed publisher
    ..A model has been proposed for cell size control in the rod-shaped cells of the fission yeast, Schizosaccharomyces pombe. This involves a protein localized to the cell ends, which inhibits mitotic activators in the middle of ..
  18. Dodgson J, Chessel A, Yamamoto M, Vaggi F, Cox S, Rosten E, et al. Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control. Nat Commun. 2013;4:1834 pubmed publisher
    ..Here we image frontally the cortex of live fission yeast cells using time-lapse and super-resolution microscopy...
  19. Yates L, Fleurdépine S, Rissland O, De Colibus L, Harlos K, Norbury C, et al. Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase. Nat Struct Mol Biol. 2012;19:782-787 pubmed publisher
    ..Here we describe crystal structures of the Schizosaccharomyces pombe cytoplasmic terminal uridylyl transferase Cid1 in two apo conformers and bound to UTP...
  20. Qu M, Rappas M, Wardlaw C, Garcia V, Ren J, Day M, et al. Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4(TopBP1). Mol Cell. 2013;51:723-736 pubmed publisher
    The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosaccharomyces pombe by physically coupling the Rad9-Rad1-Hus1 clamp, the Rad3(ATR) -Rad26(ATRIP) kinase complex, and the Crb2(53BP1) ..
  21. Pai C, Deegan R, Subramanian L, Gal C, Sarkar S, Blaikley E, et al. A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice. Nat Commun. 2014;5:4091 pubmed publisher
    ..Here we uncover a role for histone H3K36 modification in regulating DSB repair pathway choice in fission yeast. We find Set2-dependent H3K36 methylation reduces chromatin accessibility, reduces resection and promotes NHEJ,..
  22. Zofall M, Yamanaka S, REYES TURCU F, Zhang K, Rubin C, Grewal S. RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation. Science. 2012;335:96-100 pubmed publisher
    ..Here, we describe facultative heterochromatin islands in fission yeast and show that their formation at meiotic genes requires factors that eliminate meiotic messenger RNAs (mRNAs) ..
  23. Subramanian L, Toda N, Rappsilber J, Allshire R. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014;4:140043 pubmed publisher
    ..incorporation of CENP-A at centromeres depends on its chaperone HJURP(Scm3), and Mis18 in vertebrates and fission yeast. The recruitment of Mis18 and HJURP(Scm3) to centromeres is cell cycle regulated...
  24. Hálová L, Petersen J. Aurora promotes cell division during recovery from TOR-mediated cell cycle arrest by driving spindle pole body recruitment of Polo. J Cell Sci. 2011;124:3441-9 pubmed publisher
    ..In fission yeast, increased nutrient provision transiently delays mitotic onset without affecting growth...
  25. Zhang M, Wu P, Kelly F, Nurse P, Hang H. Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast. PLoS Biol. 2013;11:e1001597 pubmed publisher
    ..We propose that control of palmitoyltransferase activity levels provides a fundamental mechanism for modulating palmitoylomes and cellular functions. ..
  26. Choi E, Strålfors A, Catania S, Castillo A, Svensson J, Pidoux A, et al. Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast. PLoS Genet. 2012;8:e1002985 pubmed publisher
    ..It is known that normal centromeric DNA is transcribed in several systems including the fission yeast, Schizosaccharomyces pombe. Here, we show that factors which preserve stable histone H3 chromatin during transcription also play a ..
  27. Shepperd L, Meadows J, Sochaj A, Lancaster T, Zou J, Buttrick G, et al. Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint. Curr Biol. 2012;22:891-9 pubmed publisher
    ..We now report that phosphorylation of the conserved MELT motifs in Spc7 by Mph1 (Mps1) recruits Bub1 and Bub3 to the kinetochore and that this is required to maintain the SAC signal. ..
  28. Hiraga S, Alvino G, Chang F, Lian H, Sridhar A, Kubota T, et al. Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. Genes Dev. 2014;28:372-83 pubmed publisher
    ..b>Fission yeast and mammalian Rif1 proteins have also been implicated in regulating DNA replication...
  29. Yu Y, Kita A, Udo M, Katayama Y, Shintani M, Park K, et al. Sip1, a conserved AP-1 accessory protein, is important for Golgi/endosome trafficking in fission yeast. PLoS ONE. 2012;7:e45324 pubmed publisher
    ..that the AP-1 complex plays a role in Golgi/endosome trafficking, secretion, and vacuole fusion in fission yeast. Here, we isolated a mutant allele of its4(+)/sip1(+), which encodes a conserved AP-1 accessory protein...
  30. Oya E, Kato H, Chikashige Y, Tsutsumi C, Hiraoka Y, Murakami Y. Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways. PLoS Genet. 2013;9:e1003677 pubmed publisher
    Heterochromatin at the pericentromeric repeats in fission yeast is assembled and spread by an RNAi-dependent mechanism, which is coupled with the transcription of non-coding RNA from the repeats by RNA polymerase II...
  31. Subramanian D, Huang J, Sevugan M, Robinson R, Balasubramanian M, Tang X. Insight into actin organization and function in cytokinesis from analysis of fission yeast mutants. Genetics. 2013;194:435-46 pubmed publisher
    ..The actin cytoskeleton of the fission yeast, Schizosaccharomyces pombe, has been extensively characterized and contains structures analogous to those in other cell types...
  32. Arata M, Sato M, Yamashita A, Yamamoto M. The RNA-binding protein Spo5 promotes meiosis II by regulating cyclin Cdc13 in fission yeast. Genes Cells. 2014;19:225-38 pubmed publisher
    ..out a genetic screen to identify factors that regulate the initiation of meiosis II in the fission yeast Schizosaccharomyces pombe. We identified mutants deficient in meiosis II progression and repeatedly isolated mutants defective in ..
  33. Sansó M, Vargas Pérez I, Garcia P, Ayte J, Hidalgo E. Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombe. Mol Microbiol. 2011;82:542-54 pubmed publisher the current knowledge and future perspectives of how activation of Sty1 and Atf1 affect chromatin architecture of a large fraction of the Schizosaccharomyces pombe genome to trigger the cellular response to environmental stress.
  34. Chen Q, Pollard T. Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring. J Cell Biol. 2011;195:485-98 pubmed publisher
    We created two new mutants of fission yeast cofilin to investigate why cytokinesis in many organisms depends on this small actin-binding protein...
  35. Munoz J, Cortés J, Sipiczki M, Ramos M, Clemente Ramos J, Moreno M, et al. Extracellular cell wall ?(1,3)glucan is required to couple septation to actomyosin ring contraction. J Cell Biol. 2013;203:265-82 pubmed publisher
    ..Here we studied this process in fission yeast. The essential protein Bgs4 synthesizes the main cell wall ?(1,3)glucan...
  36. Tanae K, Horiuchi T, Matsuo Y, Katayama S, Kawamukai M. Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast. PLoS ONE. 2012;7:e30472 pubmed publisher
    ..To investigate the essential functions of Asf1 in Schizosaccharomyces pombe, asf1-ts mutants were constructed by random mutagenesis using PCR...
  37. Hickman M, Froyd C, Rusche L. Reinventing heterochromatin in budding yeasts: Sir2 and the origin recognition complex take center stage. Eukaryot Cell. 2011;10:1183-92 pubmed publisher
    ..has a distinct molecular composition compared to the more ubiquitous type of heterochromatin found in Schizosaccharomyces pombe, other fungi, animals, and plants and characterized by the presence of HP1 (heterochromatin protein 1)...
  38. Roseaulin L, Noguchi C, Martinez E, Ziegler M, Toda T, Noguchi E. Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex. PLoS Genet. 2013;9:e1003213 pubmed publisher
    ..We also suggest that this program prevents inappropriate duplication of the genome, which in turn contributes to the preservation of genomic integrity...
  39. Zhu Y, Ye Y, Wu Z, Wu J. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis. Mol Biol Cell. 2013;24:3187-204 pubmed publisher
    ..coordinates with Polo kinase Plo1 to control the medial cortical localization of anillin-like protein Mid1 in fission yeast. Here we show that an adaptor protein, Nod1, colocalizes with Gef2 in the contractile ring and its precursor ..
  40. Yamaoka T, Imada K, Fukunishi K, Yamasaki Y, Shimoda C, Nakamura T. The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation. Eukaryot Cell. 2013;12:1162-70 pubmed publisher
    ..Previous studies have revealed that, similar to synaptobrevin in other organisms, the fission yeast synaptobrevin ortholog Syb1 associates with post-Golgi secretory vesicles and is essential for cytokinesis and ..
  41. Murayama Y, Uhlmann F. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature. 2014;505:367-71 pubmed publisher
    ..loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added ..
  42. Funaya C, Samarasinghe S, Pruggnaller S, Ohta M, Connolly Y, Muller J, et al. Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe. Curr Biol. 2012;22:562-74 pubmed publisher
    ..In fission yeast, this process involves the dramatic conversion of arrays of cytoplasmic microtubules (MTs), generated from ..
  43. Fujita I, Tanaka M, Kanoh J. Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe. PLoS ONE. 2012;7:e49151 pubmed publisher
    ..In the fission yeast Schizosaccharomyces pombe, the Rap1 protein, one of the central players at the telomeres, associates with multiple proteins to ..
  44. Nakayama Y, Yoshimura K, Iida H. Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response. Nat Commun. 2012;3:1020 pubmed publisher
    ..Here we show that the fission yeast MscS homologues, designated Msy1 and Msy2, participate in the hypo-osmotic shock response by a mechanism ..
  45. Larochelle M, Lemay J, Bachand F. The THO complex cooperates with the nuclear RNA surveillance machinery to control small nucleolar RNA expression. Nucleic Acids Res. 2012;40:10240-53 pubmed publisher
    ..In contrast to its role in mRNA biogenesis, we show here that the fission yeast THO complex negatively controls the expression of non-coding small nucleolar (sno) RNAs...
  46. Kuscu C, Zaratiegui M, Kim H, Wah D, Martienssen R, Schalch T, et al. CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing. Proc Natl Acad Sci U S A. 2014;111:1795-800 pubmed publisher
    ..In Schizosaccharomyces pombe, H3K9me deposition depends on the RNAi pathway...
  47. Wang C, Wen W, Nilsson D, Sunnerhagen P, Chang T, Wang S. Analysis of stress granule assembly in Schizosaccharomyces pombe. RNA. 2012;18:694-703 pubmed publisher
    ..SGs have been primarily studied in mammalian cells. The existence of SGs in the fission yeast and the distantly related budding yeast was demonstrated only recently...
  48. Matsuzawa T, Morita T, Tanaka N, Tohda H, Takegawa K. Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe. Mol Microbiol. 2011;82:1531-44 pubmed publisher
    Although various mutant strains of the fission yeast Schizosaccharomyces pombe exhibit non-sexual flocculation, little is known about the mechanistic basis for this phenomenon, nor have genes encoding the implicated flocculin been ..
  49. Shim Y, Choi Y, Kang K, Cho K, Oh S, Lee J, et al. Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin. EMBO J. 2012;31:4375-87 pubmed publisher
    ..Here, we report that the Schizosaccharomyces pombe CHD remodeller, Hrp3, is a global regulator that drives proper nucleosome positioning and nucleosome ..
  50. Jun H, Liu J, Jeong H, Kim J, Qiao F. Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1. Genes Dev. 2013;27:1917-31 pubmed publisher
    ..However, the molecular nature of the nonextendible state is largely unknown. Here, we show that, in fission yeast, Tpz1 (the ortholog of human TPP1)-mediated complete linkage within the shelterin complex, bridging telomeric ..
  51. Nilsson D, Sunnerhagen P. Cellular stress induces cytoplasmic RNA granules in fission yeast. RNA. 2011;17:120-33 pubmed publisher
    ..Here, we demonstrate the existence of stress-induced cytoplasmic RNA granules in Schizosaccharomyces pombe. Homologs to several known protein components of mammalian processing bodies and stress granules are ..
  52. Choi E, Strålfors A, Castillo A, Durand Dubief M, Ekwall K, Allshire R. Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres. J Biol Chem. 2011;286:23600-7 pubmed publisher
    ..In fission yeast, adjacent heterochromatin can direct CENP-A(Cnp1) chromatin establishment, but the underlying features ..
  53. Basu R, Chang F. Characterization of dip1p reveals a switch in Arp2/3-dependent actin assembly for fission yeast endocytosis. Curr Biol. 2011;21:905-16 pubmed publisher
    ..What initiates Arp2/3-dependent actin polymerization in this process is not well understood. The Schizosaccharomyces pombe WISH/DIP/SPIN90 ortholog dip1p is an actin-patch protein that regulates the temporal sequence of ..
  54. Iimori M, Ozaki K, Chikashige Y, Habu T, Hiraoka Y, Maki T, et al. A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules. Exp Cell Res. 2012;318:262-75 pubmed publisher
    Mal3 is a fission yeast homolog of EB1, a plus-end tracking protein (+TIP). We have generated a mutation (89R) replacing glutamine with arginine in the calponin homology (CH) domain of Mal3...
  55. Kabeche R, Baldissard S, Hammond J, Howard L, Moseley J. The filament-forming protein Pil1 assembles linear eisosomes in fission yeast. Mol Biol Cell. 2011;22:4059-67 pubmed publisher
    ..Here we show that the core eisosome protein Pil1 forms linear cortical filaments in fission yeast cells and that purified Pil1 assembles into filaments in vitro...
  56. Nandakumar J, Cech T. DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe. Nucleic Acids Res. 2012;40:235-44 pubmed publisher
    ..While addressing the RNA discrimination properties of SpPot1, the POT1 homolog in Schizosaccharomyces pombe, we found an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide ..
  57. Yamazaki H, Tarumoto Y, Ishikawa F. Tel1(ATM) and Rad3(ATR) phosphorylate the telomere protein Ccq1 to recruit telomerase and elongate telomeres in fission yeast. Genes Dev. 2012;26:241-6 pubmed publisher
    In fission yeast, the DNA damage sensor kinases Tel1(ATM) and Rad3(ATR) exist at telomeres and are required for telomere maintenance, but the biological role they play at telomeres is not known...
  58. Saito Y, Takeda J, Okada M, Kobayashi J, Kato A, Hirota K, et al. The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination. Sci Rep. 2013;3:2022 pubmed publisher
    ..LC-MS/MS analysis identified Bag101 which binds to Rad22, the fission yeast homologue of RAD52...
  59. Coelho M, Dereli A, Haese A, Kuhn S, Malinovska L, Desantis M, et al. Fission yeast does not age under favorable conditions, but does so after stress. Curr Biol. 2013;23:1844-52 pubmed publisher
    ..We performed a complete pedigree analysis of microcolonies of the fission yeast Schizosaccharomyces pombe growing from a single cell...
  60. Sukegawa Y, Yamashita A, Yamamoto M. The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues. PLoS Genet. 2011;7:e1002387 pubmed publisher
    The RRM-type RNA-binding protein Mei2 is a master regulator of meiosis in fission yeast, in which it stabilizes meiosis-specific mRNAs by blocking their destruction...
  61. Ye Y, Lee I, Runge K, Wu J. Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis. Mol Biol Cell. 2012;23:1181-95 pubmed publisher
    ..and contractile-ring formation during cytokinesis, but how they regulate early steps of cytokinesis in fission yeast remains largely unknown...
  62. Alper B, Job G, Yadav R, Shanker S, Lowe B, Partridge J. Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast. EMBO J. 2013;32:2321-35 pubmed publisher
    Heterochromatin assembly in fission yeast depends on the Clr4 histone methyltransferase, which targets H3K9...
  63. Burke T, Christensen J, Barone E, Suarez C, Sirotkin V, Kovar D. Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers. Curr Biol. 2014;24:579-85 pubmed publisher
    ..Here we show that inhibition of Arp2/3 complex in the fission yeast Schizosaccharomyces pombe not only depletes Arp2/3-complex-mediated endocytic actin patches, but also induces a dramatic excess of ..
  64. Woolcock K, Gaidatzis D, Punga T, Buhler M. Dicer associates with chromatin to repress genome activity in Schizosaccharomyces pombe. Nat Struct Mol Biol. 2011;18:94-9 pubmed publisher
    In the fission yeast S. pombe, the RNA interference (RNAi) pathway is required to generate small interfering RNAs (siRNAs) that mediate heterochromatic silencing of centromeric repeats...
  65. Padmanabhan A, Bakka K, Sevugan M, Naqvi N, D souza V, Tang X, et al. IQGAP-related Rng2p organizes cortical nodes and ensures position of cell division in fission yeast. Curr Biol. 2011;21:467-72 pubmed publisher
    ..The fission yeast Schizosaccharomyces pombe, like animal cells, divides using an actomyosin ring and is an attractive model to study eukaryotic ..
  66. Ioannoni R, Beaudoin J, López Maury L, Codlin S, Bahler J, Labbe S. Cuf2 is a novel meiosis-specific regulatory factor of meiosis maturation. PLoS ONE. 2012;7:e36338 pubmed publisher
    ..Collectively, the results revealed that Cuf2 functions in the timely repression of the middle-phase genes during meiotic differentiation. ..
  67. Tanaka A, Tanizawa H, Sriswasdi S, Iwasaki O, Chatterjee A, Speicher D, et al. Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation. Mol Cell. 2012;48:532-46 pubmed publisher nonhomologous end joining, mediates clustering of long terminal repeat retrotransposons at centromeres in fission yeast. We demonstrate that the CENP-B subunit, Abp1, functions as a recruiter of the Ku complex, which in turn loads ..
  68. Nandi S, Whitby M. The ATPase activity of Fml1 is essential for its roles in homologous recombination and DNA repair. Nucleic Acids Res. 2012;40:9584-95 pubmed publisher
    In fission yeast, the DNA helicase Fml1, which is an orthologue of human FANCM, is a key component of the machinery that drives and governs homologous recombination (HR)...
  69. Schreiber A, Stengel F, Zhang Z, Enchev R, Kong E, Morris E, et al. Structural basis for the subunit assembly of the anaphase-promoting complex. Nature. 2011;470:227-32 pubmed publisher
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    ..predicted to be a dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) related to Pom1 in Schizosaccharomyces pombe. DYRKs share a kinase domain capable of catalyzing autophosphorylation on tyrosine and exogenous ..
  71. Sansó M, Lee K, Viladevall L, Jacques P, Page V, Nagy S, et al. A positive feedback loop links opposing functions of P-TEFb/Cdk9 and histone H2B ubiquitylation to regulate transcript elongation in fission yeast. PLoS Genet. 2012;8:e1002822 pubmed publisher
    ..Cdk9, the ortholog of metazoan positive transcription elongation factor b (P-TEFb), in the fission yeast Schizosaccharomyces pombe. Chemical and classical genetic analyses indicate that lowering Cdk9 activity or preventing ..
  72. Lorenz D, Mikheyeva I, Johansen P, Meyer L, Berg A, Grewal S, et al. CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons. Mol Cell Biol. 2012;32:4215-25 pubmed
    ..The fission yeast Schizosaccharomyces pombe homologs of mammalian CENP-B perform a host genome surveillance role by controlling Tf2 long terminal ..
  73. Wang C, Chen W, Wang S. Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe. Mol Cell Biol. 2013;33:1244-53 pubmed publisher
    ..Here, we describe the characterization of P bodies in the fission yeast Schizosaccharomyces pombe. Most information on the property and function of P bodies stems from studies in the distantly related ..
  74. Clayton J, Pollard L, Sckolnick M, Bookwalter C, Hodges A, Trybus K, et al. Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables. Mol Biol Cell. 2014;25:66-75 pubmed publisher
    ..Our previous work suggested, however, that the fission yeast myosin-V (Myo52p) is a nonprocessive motor whose activity is enhanced by tropomyosin (Cdc8p)...
  75. Syrovatkina V, Fu C, Tran P. Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation. Curr Biol. 2013;23:2423-9 pubmed publisher
    ..the effect of deleting or switching off different combinations of antagonistic force contributors in the fission yeast metaphase spindle...
  76. Hiraoka Y, Maekawa H, Asakawa H, Chikashige Y, Kojidani T, Osakada H, et al. Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres. Genes Cells. 2011;16:1000-11 pubmed publisher
    ..In the fission yeast Schizosaccharomyces pombe, Sad1, an INM protein of the conserved SUN-domain family, plays an active role in moving chromosomes ..
  77. Chen J, Lu L, Ohi M, Creamer K, English C, Partridge J, et al. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011;195:583-93 pubmed publisher
    ..In this paper, we characterize a new kinetochore substrate of fission yeast Cdk1, Nsk1, which promotes proper kinetochore-MT (k-MT) interactions and chromosome movements in a ..
  78. de Medina Redondo M, Arnaiz Pita Y, Clavaud C, Fontaine T, del Rey F, Latge J, et al. ?(1,3)-glucanosyl-transferase activity is essential for cell wall integrity and viability of Schizosaccharomyces pombe. PLoS ONE. 2010;5:e14046 pubmed publisher
    The formation of the cell wall in Schizosaccharomyces pombe requires the coordinated activity of enzymes involved in the biosynthesis and modification of ?-glucans...
  79. Fujita I, Nishihara Y, Tanaka M, Tsujii H, Chikashige Y, Watanabe Y, et al. Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation. Curr Biol. 2012;22:1932-7 pubmed publisher
    ..Here, we report the spatiotemporal regulation of telomeres in Schizosaccharomyces pombe closed mitosis...
  80. Pointner J, Persson J, Prasad P, Norman Axelsson U, Strålfors A, Khorosjutina O, et al. CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe. EMBO J. 2012;31:4388-403 pubmed publisher
    ..We find a striking evolutionary shift in remodeler usage between budding and fission yeast. The S...
  81. Iraqui I, Chekkal Y, Jmari N, Pietrobon V, Fréon K, Costes A, et al. Recovery of arrested replication forks by homologous recombination is error-prone. PLoS Genet. 2012;8:e1002976 pubmed publisher
    ..Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including ..
  82. Maurer S, Fourniol F, Bohner G, Moores C, Surrey T. EBs recognize a nucleotide-dependent structural cap at growing microtubule ends. Cell. 2012;149:371-82 pubmed publisher
    ..and fluorescence imaging, we present a pseudoatomic model of how the calponin homology (CH) domain of the fission yeast EB Mal3 binds to the end regions of growing microtubules...
  83. Nandakumar J, Cech T. Finding the end: recruitment of telomerase to telomeres. Nat Rev Mol Cell Biol. 2013;14:69-82 pubmed publisher
    ..into the mechanisms of telomerase recruitment to telomeres, highlighting the contribution of telomere-associated proteins, including TPP1 in humans, Ccq1 in Schizosaccharomyces pombe and Cdc13 and Ku70-Ku80 in Saccharomyces cerevisiae.
  84. Matsuo Y, Nishino K, Mizuno K, Akihiro T, Toda T, Matsuo Y, et al. Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast. PLoS ONE. 2013;8:e59887 pubmed publisher
    Polypeptone is widely excluded from Schizosaccharomyces pombe growth medium. However, the reasons why polypeptone should be avoided have not been documented...
  85. Lunde B, Magler I, Meinhart A. Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity. Nucleic Acids Res. 2012;40:9815-24 pubmed publisher
    ..By mutating this histidine to an asparagine residue in Cid1, we diminished Cid1's activity for UTP addition and improved ATP incorporation, supporting that this residue is important for UTP selectivity. ..
  86. Bilokapic S, Schwartz T. Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex. Proc Natl Acad Sci U S A. 2012;109:15241-6 pubmed
    ..We present the crystal structure of Schizosaccharomyces pombe Nup37 in complex with Nup120, a 174-kDa subassembly that forms one of the two short arms of the Y-..
  87. Johnson A, Gould K. Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. EMBO J. 2011;30:341-54 pubmed publisher
    ..In the fission yeast, Schizosaccharomyces pombe, the checkpoint protein and E3 ubiquitin ligase, Dma1, delays cytokinesis by inhibiting the septation ..
  88. Nakase M, Nakase Y, Chardwiriyapreecha S, Kakinuma Y, Matsumoto T, Takegawa K. Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p. Microbiology. 2012;158:659-73 pubmed publisher
    In Schizosaccharomyces pombe, neither intracellular sorting nor ubiquitination of amino acid permeases is well understood. In the present study, we show that intracellular sorting of the amino acid permease Aat1p in S...
  89. Liu G, Young D. Conserved Orb6 phosphorylation sites are essential for polarized cell growth in Schizosaccharomyces pombe. PLoS ONE. 2012;7:e37221 pubmed publisher
    The Ndr-related Orb6 kinase is a key regulator of polarized cell growth in fission yeast, however the mechanism of Orb6 activation is unclear...
  90. Tazumi A, Fukuura M, Nakato R, Kishimoto A, Takenaka T, Ogawa S, et al. Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast. Genes Dev. 2012;26:2050-62 pubmed publisher
    ..we report that the telomere-binding protein Taz1 plays a crucial role in the control of replication timing in fission yeast. A DNA element located proximal to a late origin in the chromosome arm represses initiation from the origin in ..
  91. Strålfors A, Walfridsson J, Bhuiyan H, Ekwall K. The FUN30 chromatin remodeler, Fft3, protects centromeric and subtelomeric domains from euchromatin formation. PLoS Genet. 2011;7:e1001334 pubmed publisher
    ..Our data strongly suggest that Fft3 controls the identity of chromatin domains by protecting these regions from euchromatin assembly...
  92. Hachet O, Berthelot Grosjean M, Kokkoris K, Vincenzetti V, Moosbrugger J, Martin S. A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane. Cell. 2011;145:1116-28 pubmed publisher
    Concentration gradients regulate many cell biological and developmental processes. In rod-shaped fission yeast cells, polar cortical gradients of the DYRK family kinase Pom1 couple cell length with mitotic commitment by inhibiting a ..