Neurospora crassa OR74A


Alias: Neurospora crassa 74-OR23-1V A, Neurospora crassa 74-OR23-1VA, Neurospora crassa FGSC 2489, Neurospora crassa FGSC 9013, Neurospora crassa N150

Top Publications

  1. He Q, Liu Y. Molecular mechanism of light responses in Neurospora: from light-induced transcription to photoadaptation. Genes Dev. 2005;19:2888-99 pubmed
    ..Finally, phosphorylation was also shown to inhibit the LRE-binding activity of D-WCC (dark WC complex), suggesting that it plays an important role in the circadian negative feedback loop. ..
  2. Baker C, Kettenbach A, Loros J, Gerber S, Dunlap J. Quantitative proteomics reveals a dynamic interactome and phase-specific phosphorylation in the Neurospora circadian clock. Mol Cell. 2009;34:354-63 pubmed publisher
  3. Znameroski E, Coradetti S, Roche C, Tsai J, Iavarone A, Cate J, et al. Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins. Proc Natl Acad Sci U S A. 2012;109:6012-7 pubmed publisher
    ..Thus, the ability to induce cellulase gene expression using a common and soluble carbon source simplifies enzyme production and characterization, which could be applied to other cellulolytic filamentous fungi. ..
  4. Youssar L, Schmidhauser T, Avalos J. The Neurospora crassa gene responsible for the cut and ovc phenotypes encodes a protein of the haloacid dehalogenase family. Mol Microbiol. 2005;55:828-38 pubmed
    ..The cut-1 promoter contains putative regulatory elements involved in osmotic or thermal stress. We show that cut-1 transcription is low in illuminated or dark-grown cultures, and is induced by high osmotic conditions or by heat shock. ..
  5. He Q, Shu H, Cheng P, Chen S, Wang L, Liu Y. Light-independent phosphorylation of WHITE COLLAR-1 regulates its function in the Neurospora circadian negative feedback loop. J Biol Chem. 2005;280:17526-32 pubmed
    ..Together, these data suggest that phosphorylation of these sites negatively regulates the function of WC-1 in the circadian negative feedback loop and is important for the function of the Neurospora circadian clock. ..
  6. Fleissner A, Sarkar S, Jacobson D, Roca M, Read N, Glass N. The so locus is required for vegetative cell fusion and postfertilization events in Neurospora crassa. Eukaryot Cell. 2005;4:920-30 pubmed
    ..Database searches showed that so was conserved in the genomes of filamentous ascomycete fungi but was absent in ascomycete yeast and basidiomycete species. ..
  7. He Q, Liu Y. Degradation of the Neurospora circadian clock protein FREQUENCY through the ubiquitin-proteasome pathway. Biochem Soc Trans. 2005;33:953-6 pubmed
    ..In addition, these findings resolve the CSN paradox and suggest that the major function of CSN is to maintain the stability of SCF ubiquitin ligases in vivo. ..
  8. Rasmussen C, Glass N. A Rho-type GTPase, rho-4, is required for septation in Neurospora crassa. Eukaryot Cell. 2005;4:1913-25 pubmed
    ..Characterization of strains containing activated alleles of rho-4 showed that RHO-4-GTP is likely to initiate new septum formation in N. crassa. ..
  9. Kaneko I, Dementhon K, Xiang Q, Glass N. Nonallelic interactions between het-c and a polymorphic locus, pin-c, are essential for nonself recognition and programmed cell death in Neurospora crassa. Genetics. 2006;172:1545-55 pubmed
    ..These data suggest that nonallelic interactions may be important in nonself recognition in filamentous fungi and that proteins containing a HET domain may be a key factor in these interactions. ..

More Information

Publications112 found, 100 shown here

  1. Hunt S, Elvin M, Crosthwaite S, Heintzen C. The PAS/LOV protein VIVID controls temperature compensation of circadian clock phase and development in Neurospora crassa. Genes Dev. 2007;21:1964-74 pubmed
    ..Thus, temperature compensation of clock-controlled output is a key factor in maintaining temperature compensation of the entire circadian system. ..
  2. Honda S, Selker E. Direct interaction between DNA methyltransferase DIM-2 and HP1 is required for DNA methylation in Neurospora crassa. Mol Cell Biol. 2008;28:6044-55 pubmed publisher
  3. Cha J, Chang S, Huang G, Cheng P, Liu Y. Control of WHITE COLLAR localization by phosphorylation is a critical step in the circadian negative feedback process. EMBO J. 2008;27:3246-55 pubmed publisher
  4. Hutchison E, Glass N. Meiotic regulators Ndt80 and ime2 have different roles in Saccharomyces and Neurospora. Genetics. 2010;185:1271-82 pubmed publisher
    ..Our data indicate that the IME2/NDT80 pathway is not involved in meiosis in N. crassa, but rather regulates the formation of female reproductive structures. ..
  5. Tian C, Li J, Glass N. Exploring the bZIP transcription factor regulatory network in Neurospora crassa. Microbiology. 2011;157:747-59 pubmed publisher
    ..These data indicate how N. crassa responds to stress and provide information on pathway evolution. ..
  6. Belden W, Lewis Z, Selker E, Loros J, Dunlap J. CHD1 remodels chromatin and influences transient DNA methylation at the clock gene frequency. PLoS Genet. 2011;7:e1002166 pubmed publisher
    ..These data demonstrate that the epigenetic state of clock genes is dependent on normal regulation of clock components. ..
  7. Sun J, Tian C, Diamond S, Glass N. Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa. Eukaryot Cell. 2012;11:482-93 pubmed publisher
    ..This systematic analysis illustrates the similarities and differences in regulation of hemicellulose degradation among filamentous fungi. ..
  8. Richthammer C, Enseleit M, Sánchez León E, März S, Heilig Y, Riquelme M, et al. RHO1 and RHO2 share partially overlapping functions in the regulation of cell wall integrity and hyphal polarity in Neurospora crassa. Mol Microbiol. 2012;85:716-33 pubmed publisher
    ..We show that RHO2 does not regulate glucan synthase activity and the actin cytoskeleton, but physically interacts with PKC1 to regulate the cell wall integrity pathway. ..
  9. Schafmeier T, Haase A, Káldi K, Scholz J, Fuchs M, Brunner M. Transcriptional feedback of Neurospora circadian clock gene by phosphorylation-dependent inactivation of its transcription factor. Cell. 2005;122:235-46 pubmed
    ..We propose that negative feedback in the circadian clock of Neurospora is mediated by FRQ, which rhythmically promotes phosphorylation of WCC, functionally equivalent to a cyclin recruiting cyclin-dependent kinase to its targets. ..
  10. Yamashita K, Shiozawa A, Watanabe S, Fukumori F, Kimura M, Fujimura M. ATF-1 transcription factor regulates the expression of ccg-1 and cat-1 genes in response to fludioxonil under OS-2 MAP kinase in Neurospora crassa. Fungal Genet Biol. 2008;45:1562-9 pubmed publisher
    ..These findings suggest that ATF-1 acts as one of the transcriptional factors downstream of the OS-2 MAP kinase and probably regulates some genes involved in conidiation, circadian rhythm, and ascospore maturation in N. crassa. ..
  11. Araujo Palomares C, Richthammer C, Seiler S, Castro Longoria E. Functional characterization and cellular dynamics of the CDC-42 - RAC - CDC-24 module in Neurospora crassa. PLoS ONE. 2011;6:e27148 pubmed publisher
    ..In summary, this study identifies CDC-24 as essential regulator for RAC and CDC-42 that have common and distinct functions during polarity establishment and maintenance of cell polarity in N. crassa. ..
  12. Dettmann A, Heilig Y, Ludwig S, Schmitt K, Illgen J, Fleißner A, et al. HAM-2 and HAM-3 are central for the assembly of the Neurospora?STRIPAK complex at the nuclear envelope and regulate nuclear accumulation of the MAP kinase MAK-1 in a MAK-2-dependent manner. Mol Microbiol. 2013;90:796-812 pubmed publisher
  13. Schafmeier T, Káldi K, Diernfellner A, Mohr C, Brunner M. Phosphorylation-dependent maturation of Neurospora circadian clock protein from a nuclear repressor toward a cytoplasmic activator. Genes Dev. 2006;20:297-306 pubmed
    ..Phosphorylation of FRQ at the PEST-2 region triggers its maturation from a nuclear repressor toward a cytoplasmic activator...
  14. Aldabbous M, Roca M, Stout A, Huang I, Read N, Free S. The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa. Microbiology. 2010;156:2621-9 pubmed publisher
    ..ham-5 deletion mutants had a reduced rate of hyphal extension and altered hyphal morphology, and were unable to produce the conidial anastomosis tubes that are required for hyphal fusion during colony initiation...
  15. Simonin A, Rasmussen C, Yang M, Glass N. Genes encoding a striatin-like protein (ham-3) and a forkhead associated protein (ham-4) are required for hyphal fusion in Neurospora crassa. Fungal Genet Biol. 2010;47:855-68 pubmed publisher
    ..These data indicate that, similar to humans, the HAM proteins may form different signaling complexes that are important during both vegetative and sexual development in N. crassa...
  16. Malzahn E, Ciprianidis S, Káldi K, Schafmeier T, Brunner M. Photoadaptation in Neurospora by competitive interaction of activating and inhibitory LOV domains. Cell. 2010;142:762-72 pubmed publisher
    ..We show that VVD is essential to discriminate between day and night, even in naturally ambiguous photoperiods with moonlight...
  17. Coradetti S, Craig J, Xiong Y, Shock T, Tian C, Glass N. Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi. Proc Natl Acad Sci U S A. 2012;109:7397-402 pubmed publisher
    ..Further manipulation of this control system in industrial production strains may significantly improve yields of cellulases for cellulosic biofuel production...
  18. Schürg T, Brandt U, Adis C, Fleissner A. The Saccharomyces cerevisiae BEM1 homologue in Neurospora crassa promotes co-ordinated cell behaviour resulting in cell fusion. Mol Microbiol. 2012;86:349-66 pubmed publisher
    ..By genetically dissecting the contribution of BEM1 to additional various polarization events, we also obtained first hints that BEM1 might function in different protein complexes controlling polarity and growth direction...
  19. Coradetti S, Xiong Y, Glass N. Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa. Microbiologyopen. 2013;2:595-609 pubmed publisher
    ..However, this functionality may require additional engineering in some species. ..
  20. Managadze D, W rtz C, Sichting M, Niehaus G, Veenhuis M, Rottensteiner H. The peroxin PEX14 of Neurospora crassa is essential for the biogenesis of both glyoxysomes and Woronin bodies. Traffic. 2007;8:687-701 pubmed publisher
    ..Our data support the view that Woronin bodies emerge from glyoxysomes through import of HEX1 and subsequent fission...
  21. Richter N, Hummel W. Biochemical characterisation of a NADPH-dependent carbonyl reductase from Neurospora crassa reducing ?- and ?-keto esters. Enzyme Microb Technol. 2011;48:472-9 pubmed publisher
    ..8%) to -3 °C (98.0%). When the experimental conditions were optimised to improve the optical purity of the product, (S)-4-chloro-3-hydroxybutanoate (ee 98.0%) was successfully produced on a 300 mg (1.8 mmol) scale using NcCR at -3 °C. ..
  22. Sun P, Zhou X, Wang Z. [Application of bioinformatics analysis of signal peptide in the identification of Neurospora crassa phyA gene]. Nan Fang Yi Ke Da Xue Xue Bao. 2009;29:1098-101 pubmed
    ..The signal peptide, the cleavage site and the secretion pathway were determined, and the expressed recombinant protein with 54,000 displayed phytase activity. The gene has been identified to encode N. crassa phyA. ..
  23. Summers W, Wilkins J, Dwivedi R, Ezzati P, Court D. Mitochondrial dysfunction resulting from the absence of mitochondrial porin in Neurospora crassa. Mitochondrion. 2012;12:220-9 pubmed publisher
    ..Transcriptional and post-transcriptional mechanisms contribute to the response, reflecting the extent of porin influence. ..
  24. Flores Alvarez L, Corrales Escobosa A, Cortes Penagos C, Martínez Pacheco M, Wrobel Zasada K, Wrobel Kaczmarczyk K, et al. The Neurospora crassa chr-1 gene is up-regulated by chromate and its encoded CHR-1 protein causes chromate sensitivity and chromium accumulation. Curr Genet. 2012;58:281-90 pubmed publisher
    ..This is the first report assigning a role as a chromate transporter to a nonbacterial CHR protein. ..
  25. Bowring F, Yeadon P, Catcheside D. Residual recombination in Neurospora crassa spo11 deletion homozygotes occurs during meiosis. Mol Genet Genomics. 2013;288:437-44 pubmed publisher
  26. Sasaki T, Lynch K, Mueller C, Friedman S, Freitag M, Lewis Z. Heterochromatin controls ?H2A localization in Neurospora crassa. Eukaryot Cell. 2014;13:990-1000 pubmed publisher
    ..Together, these data suggest that heterochromatin formation is essential for normal DNA replication or repair. ..
  27. Lin L, Chen Y, Li J, Wang S, Sun W, Tian C. Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa. Biotechnol Lett. 2017;39:545-551 pubmed publisher
    ..NCW-1 is a novel component that plays a critical role in the cellulase induction signaling pathway. ..
  28. Wang B, Zhou X, Loros J, Dunlap J. Alternative Use of DNA Binding Domains by the Neurospora White Collar Complex Dictates Circadian Regulation and Light Responses. Mol Cell Biol. 2015;36:781-93 pubmed publisher
    ..The data suggest a means by which alterations in the tertiary and quaternary structures of the WCC can lead to its distinct functions in the dark and in the light. ..
  29. Yoshida Y, Ogura Y, Hasunuma K. Interaction of nucleoside diphosphate kinase and catalases for stress and light responses in Neurospora crassa. FEBS Lett. 2006;580:3282-6 pubmed publisher
    ..We found, by conducting a yeast two-hybrid assay, that Cat-1 interacted with NDK-1. NDK-1 was suggested to control Cat-1 and Cat-3 at the post-transcriptional level in response to heat, oxidative stress and light...
  30. Lamb J, Zoltowski B, Pabit S, Li L, Crane B, Pollack L. Illuminating solution responses of a LOV domain protein with photocoupled small-angle X-ray scattering. J Mol Biol. 2009;393:909-19 pubmed publisher
    ..Envelope reconstructions of the transient light-state dimer reveal structures that are best described by a parallel arrangement of subunits that have significantly changed conformation compared to the crystal structure...
  31. Gessmann D, Flinner N, Pfannstiel J, Schlösinger A, Schleiff E, Nussberger S, et al. Structural elements of the mitochondrial preprotein-conducting channel Tom40 dissolved by bioinformatics and mass spectrometry. Biochim Biophys Acta. 2011;1807:1647-57 pubmed publisher
    ..The outer surface of the Tom40 barrel reveals two conserved amino acid clusters. They may be involved in binding other components of the TOM complex or bridging components of the TIM machinery of the mitochondrial inner membrane. ..
  32. Wakabayashi M, Saijyou N, Hatakeyama S, Inoue H, Tanaka S. Neurospora mrc1 homologue is involved in replication stability and is required for normal cell growth and chromosome integrity in mus-9 and mus-21 mutants. Fungal Genet Biol. 2012;49:263-70 pubmed publisher
    ..These results imply that activation of the checkpoint pathway can protect cells from instability of DNA replication caused by loss of mrc1. ..
  33. Smith R, Wellman K, Smith M. Trans-species activity of a nonself recognition domain. BMC Microbiol. 2013;13:63 pubmed publisher
    ..Finally, our results suggest that variations on the PA incompatibility domain may serve as novel and specific antimicrobial peptides. ..
  34. Tang S, Bubner P, Bauer S, Somerville C. O-Glycan analysis of cellobiohydrolase I from Neurospora crassa. Glycobiology. 2016;26:670-7 pubmed publisher
    ..crassa, the knowledge of the CBHI O-glycans may enable the future evaluation of the role of O-glycosylation on cellulase function and the development of directed O-glycan/cellulase engineering. ..
  35. Francis K, Russell B, Gadda G. Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase. J Biol Chem. 2005;280:5195-204 pubmed publisher
  36. Krystofova S, Borkovich K. The heterotrimeric G-protein subunits GNG-1 and GNB-1 form a Gbetagamma dimer required for normal female fertility, asexual development, and galpha protein levels in Neurospora crassa. Eukaryot Cell. 2005;4:365-78 pubmed publisher
    ..Direct evidence for a physical association between GNB-1 and GNG-1 in vivo was provided by coimmunoprecipitation...
  37. Hong C, Ruoff P, Loros J, Dunlap J. Closing the circadian negative feedback loop: FRQ-dependent clearance of WC-1 from the nucleus. Genes Dev. 2008;22:3196-204 pubmed publisher
    ..Moreover, and consistent with a noncatalytic clearance-based model for inhibition, appreciable amounts of the nuclear FRQ:WCC complex accumulate at some times of day, comprising as much as 10% of the nuclear WC-1...
  38. Deka R, Kumar R, Tamuli R. Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival. Genetica. 2011;139:885-94 pubmed publisher
    ..Crosses homozygous for ?NCU04379.2 mutant strains were fully fertile; however, we found evidence for involvement of Ca(2+)/calmodulin-dependent protein kinase encoding genes NCU02283 and NCU09123 in sexual development...
  39. Bowman B, Abreu S, Johl J, Bowman E. The pmr gene, encoding a Ca2+-ATPase, is required for calcium and manganese homeostasis and normal development of hyphae and conidia in Neurospora crassa. Eukaryot Cell. 2012;11:1362-70 pubmed publisher
  40. Wiest A, Barchers D, Eaton M, Henderson R, Schnittker R, McCluskey K. Molecular analysis of intragenic recombination at the tryptophan synthetase locus in Neurospora crassa. J Genet. 2013;92:523-8 pubmed
    ..This work defines 14 alleles of the N. crassa trp-3 gene. ..
  41. Chen Y, Pieuchot L, Loh R, Yang J, Kari T, Wong J, et al. Hydrophobic handoff for direct delivery of peroxisome tail-anchored proteins. Nat Commun. 2014;5:5790 pubmed publisher
    ..Together, these data support a mechanism in which hydrophobic moieties in the TMD chaperone and its membrane-associated receptor act in a concerted manner to prompt TMD release and membrane insertion. ..
  42. Dasgupta A, Chen C, Lee C, Gladfelter A, Dunlap J, Loros J. Biological Significance of Photoreceptor Photocycle Length: VIVID Photocycle Governs the Dynamic VIVID-White Collar Complex Pool Mediating Photo-adaptation and Response to Changes in Light Intensity. PLoS Genet. 2015;11:e1005215 pubmed publisher
    ..The great diversity in photocycle kinetics among photoreceptors may be viewed as reflecting adaptive responses to specific and salient tasks required by organisms to respond to different photic environments. ..
  43. Castrillo M, Bernhardt A, Ávalos J, Batschauer A, Pokorny R. Biochemical Characterization of the DASH-Type Cryptochrome CryD From Fusarium fujikuroi. Photochem Photobiol. 2015;91:1356-67 pubmed publisher
    ..Moreover, binding of FfCryD to RNA indicates a putative role in RNA metabolism or in posttranscriptional control of gene expression. ..
  44. Wang Y, Dong Q, Ding Z, Gai K, Han X, Kaleri F, et al. Regulation of Neurospora Catalase-3 by global heterochromatin formation and its proximal heterochromatin region. Free Radic Biol Med. 2016;99:139-152 pubmed publisher
    ..Our study indicates that the local chromatin structure creates a heterochromatin repressive environment to repress nearby gene expression. ..
  45. Li S, Lakin Thomas P. Effects of prd circadian clock mutations on FRQ-less rhythms in Neurospora. J Biol Rhythms. 2010;25:71-80 pubmed publisher
    ..We propose that prd-1 and prd-2 are good candidates for components of the FRQ-less oscillator and that prd-3 and prd-4 act on the system mainly through effects on FRQ/WCC...
  46. Pereira B, Videira A, Duarte M. Novel insights into the role of Neurospora crassa NDUFAF2, an evolutionarily conserved mitochondrial complex I assembly factor. Mol Cell Biol. 2013;33:2623-34 pubmed publisher
    ..4 (NDUFA12), 18.4 (NDUFS6), and 21 (NDUFS4) kDa. Our results demonstrate that the 13.4 L protein is a complex I assembly factor functionally conserved from fungi to mammals...
  47. Huang G, He Q, Guo J, Cha J, Liu Y. The Ccr4-not protein complex regulates the phase of the Neurospora circadian clock by controlling white collar protein stability and activity. J Biol Chem. 2013;288:31002-9 pubmed publisher
    ..Together, our findings suggest that the Ccr4-Not complex participates in the Neurospora clock function by interacting with and regulating the WC complex. ..
  48. Lauinger L, Diernfellner A, Falk S, Brunner M. The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa. Nat Commun. 2014;5:3598 pubmed publisher
    ..We show that the affinity of CK1a for FRQ decreases with increasing FRQ phosphorylation, suggesting functional inactivation of FRQ in the negative feedback loop of the circadian clock before and independent of its degradation. ..
  49. Barman A, Tamuli R. Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance. J Microbiol. 2015;53:226-35 pubmed publisher
    ..Thus, this study revealed multiple cellular roles for plc-1, splA2, and cpe-1 genes in regulation of [Ca(2+)](c), carotenoid accumulation, survival under stress conditions, and acquisition of thermotolerance induced by heat shock. ..
  50. Zanphorlin L, Lima T, Wong M, Balbuena T, Minetti C, Remeta D, et al. Heat Shock Protein 90 kDa (Hsp90) Has a Second Functional Interaction Site with the Mitochondrial Import Receptor Tom70. J Biol Chem. 2016;291:18620-31 pubmed publisher
    ..Collectively, our findings provide significant insight on the mechanisms by which preproteins interact with Hsp90 and are translocated via Tom70 to the mitochondria. ..
  51. Balasus D, Way M, Fusilli C, Mazza T, Morgan M, Cervello M, et al. The association of variants in PNPLA3 and GRP78 and the risk of developing hepatocellular carcinoma in an Italian population. Oncotarget. 2016;7:86791-86802 pubmed publisher
    ..The combinatorial predictive model developed to include these genetic variants may, if validated in independent cohorts, allow for earlier diagnosis of HCC. ..
  52. Chae M, Nargang C, Cleary I, Lin C, Todd A, Nargang F. Two zinc-cluster transcription factors control induction of alternative oxidase in Neurospora crassa. Genetics. 2007;177:1997-2006 pubmed publisher
    ..Both proteins contain potential PAS domains near their C terminus, which are found primarily in proteins involved in signal transduction...
  53. Dekhang R, Wu C, Smith K, Lamb T, Peterson M, Bredeweg E, et al. The Neurospora Transcription Factor ADV-1 Transduces Light Signals and Temporal Information to Control Rhythmic Expression of Genes Involved in Cell Fusion. G3 (Bethesda). 2017;7:129-142 pubmed publisher
    ..These data suggest that a complex regulatory network downstream of ADV-1 functions to generate distinct temporal dynamics of target gene expression relative to the central clock mechanism. ..
  54. Yuan P, Jedd G, Kumaran D, Swaminathan S, Shio H, Hewitt D, et al. A HEX-1 crystal lattice required for Woronin body function in Neurospora crassa. Nat Struct Biol. 2003;10:264-70 pubmed publisher
    ..Thus, we propose that a new function has evolved following duplication of an ancestral eIF-5A gene and that this may define an important step in fungal evolution...
  55. Bailey Shrode L, Ebbole D. The fluffy gene of Neurospora crassa is necessary and sufficient to induce conidiophore development. Genetics. 2004;166:1741-9 pubmed
  56. Pregueiro A, Price Lloyd N, Bell Pedersen D, Heintzen C, Loros J, Dunlap J. Assignment of an essential role for the Neurospora frequency gene in circadian entrainment to temperature cycles. Proc Natl Acad Sci U S A. 2005;102:2210-5 pubmed publisher
    ..Examination of several other phase markers as well as results of additional experimental tests indicate that the FLO is, at best, a slave oscillator to the TTFL, which underlies circadian rhythm generation in Neurospora...
  57. Becker L, Bannwarth M, Meisinger C, Hill K, Model K, Krimmer T, et al. Preprotein translocase of the outer mitochondrial membrane: reconstituted Tom40 forms a characteristic TOM pore. J Mol Biol. 2005;353:1011-20 pubmed publisher
  58. Runke G, Maier E, Summers W, Bay D, Benz R, Court D. Deletion variants of Neurospora mitochondrial porin: electrophysiological and spectroscopic analysis. Biophys J. 2006;90:3155-64 pubmed publisher
    ..Based on these results, modifications to the existing models for porin structure are proposed...
  59. Rasmussen C, Glass N. Localization of RHO-4 indicates differential regulation of conidial versus vegetative septation in the filamentous fungus Neurospora crassa. Eukaryot Cell. 2007;6:1097-107 pubmed publisher
    ..These data highlight the differences in the regulation of septation during conidiation versus vegetative septation in filamentous fungi...
  60. Managadze D, W rtz C, Wiese S, Schneider M, Girzalsky W, Meyer H, et al. Identification of PEX33, a novel component of the peroxisomal docking complex in the filamentous fungus Neurospora crassa. Eur J Cell Biol. 2010;89:955-64 pubmed publisher
    ..As the function of PEX33 was not redundant to that of PEX14, it is a genuine novel peroxin. Based on our experimental data, the function of PEX33 seems to resemble that of yeast PEX17 despite clear structural differences...
  61. Hall C, Welch J, Kowbel D, Glass N. Evolution and diversity of a fungal self/nonself recognition locus. PLoS ONE. 2010;5:e14055 pubmed publisher
    ..Distinct allele classes can emerge by recombination and positive selection and are subsequently maintained by balancing selection and divergence of intergenic sequence resulting in recombination blocks between haplotypes. ..
  62. Pomraning K, Smith K, Freitag M. Bulk segregant analysis followed by high-throughput sequencing reveals the Neurospora cell cycle gene, ndc-1, to be allelic with the gene for ornithine decarboxylase, spe-1. Eukaryot Cell. 2011;10:724-33 pubmed publisher
    ..Based on our results, we propose changing ndc-1 to spe-1(ndc), which reflects that this mutation results in an ODC with a specific nuclear division defect...
  63. Caster S, Castillo K, Sachs M, Bell Pedersen D. Circadian clock regulation of mRNA translation through eukaryotic elongation factor eEF-2. Proc Natl Acad Sci U S A. 2016;113:9605-10 pubmed publisher
    ..In contrast, rhythms in phosphorylated eEF-2 levels are not necessary for rhythms in accumulation of the clock protein FRQ, indicating that clock control of eEF-2 activity promotes rhythmic translation of specific mRNAs. ..
  64. Fuchs F, Westermann B. Role of Unc104/KIF1-related motor proteins in mitochondrial transport in Neurospora crassa. Mol Biol Cell. 2005;16:153-61 pubmed publisher
  65. Rerngsamran P, Murphy M, Doyle S, Ebbole D. Fluffy, the major regulator of conidiation in Neurospora crassa, directly activates a developmentally regulated hydrophobin gene. Mol Microbiol. 2005;56:282-97 pubmed publisher
    ..In addition, yeast one hybrid experiments demonstrated that the C-terminal portion of FL functions in transcriptional activation. Transcriptional profiling was used to identify additional potential targets for regulation by fl...
  66. Olmedo M, Navarro Sampedro L, Ruger Herreros C, Kim S, Jeong B, Lee B, et al. A role in the regulation of transcription by light for RCO-1 and RCM-1, the Neurospora homologs of the yeast Tup1-Ssn6 repressor. Fungal Genet Biol. 2010;47:939-52 pubmed publisher
    ..We show that RCO-1 and RCM-1 participate in the light-transduction pathway of Neurospora and has a role in photoadaptation by repressing gene transcription after long exposures to light...
  67. Yamamoto H, Itoh N, Kawano S, Yatsukawa Y, Momose T, Makio T, et al. Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria. Proc Natl Acad Sci U S A. 2011;108:91-6 pubmed publisher
    ..Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency...
  68. Zoltowski B, Schwerdtfeger C, Widom J, Loros J, Bilwes A, Dunlap J, et al. Conformational switching in the fungal light sensor Vivid. Science. 2007;316:1054-7 pubmed publisher
    ..Key elements of this activation mechanism are conserved by other photosensors such as White Collar-1, ZEITLUPE, ENVOY, and flavin-binding, kelch repeat, F-BOX 1 (FKF1)...
  69. Yamashita K, Shiozawa A, Banno S, Fukumori F, Ichiishi A, Kimura M, et al. Involvement of OS-2 MAP kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa. Genes Genet Syst. 2007;82:301-10 pubmed
  70. Rauch G, Ehammer H, Bornemann S, Macheroux P. Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity. FEBS J. 2008;275:1464-73 pubmed publisher
  71. Kwan A, Macindoe I, Vukasin P, Morris V, Kass I, Gupte R, et al. The Cys3-Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity. J Mol Biol. 2008;382:708-20 pubmed publisher
  72. Ding S, Liu W, Iliuk A, Ribot C, Vallet J, Tao A, et al. The tig1 histone deacetylase complex regulates infectious growth in the rice blast fungus Magnaporthe oryzae. Plant Cell. 2010;22:2495-508 pubmed publisher
    ..oryzae and highlighted that chromatin modification is an essential regulatory mechanism during plant infection...
  73. Chung D, Greenwald C, Upadhyay S, Ding S, Wilkinson H, Ebbole D, et al. acon-3, the Neurospora crassa ortholog of the developmental modifier, medA, complements the conidiation defect of the Aspergillus nidulans mutant. Fungal Genet Biol. 2011;48:370-6 pubmed publisher
    ..Nonetheless, expression of acon-3 using its native promoter complemented the conidiation defects of the A. nidulans ?medA and medA15 mutants. We conclude that the biochemical activity of the medA orthologs is conserved for conidiation...
  74. Cha J, Yuan H, Liu Y. Regulation of the activity and cellular localization of the circadian clock protein FRQ. J Biol Chem. 2011;286:11469-78 pubmed publisher
    ..Our results indicate that the rhythmic FRQ phosphorylation profile observed is an important part of the negative feedback mechanism that drives robust circadian gene expression...
  75. Zhou Z, Liu X, Hu Q, Zhang N, Sun G, Cha J, et al. Suppression of WC-independent frequency transcription by RCO-1 is essential for Neurospora circadian clock. Proc Natl Acad Sci U S A. 2013;110:E4867-74 pubmed publisher
    ..Together, our results uncover a previously unexpected regulatory mechanism for clock gene transcription...
  76. Yang Q, Poole S, Borkovich K. A G-protein beta subunit required for sexual and vegetative development and maintenance of normal G alpha protein levels in Neurospora crassa. Eukaryot Cell. 2002;1:378-90 pubmed
    ..In contrast, many other delta gnb-1 phenotypes, including female sterility and defective conidiation, can be explained by altered levels of the three N. crassa G alpha proteins...
  77. Faou P, Tropschug M. Neurospora crassa CyPBP37: a cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B1. J Mol Biol. 2004;344:1147-57 pubmed publisher
    ..In addition to its function in thiazole synthesis, CyPBP37 is a stress-inducible protein. N.crassa cyclophilin41 can chaperone the folding of CyPBP37, its own binding partner...
  78. Suzuki K, Kato A, Sakuraba Y, Inoue H. Srs2 and RecQ homologs cooperate in mei-3-mediated homologous recombination repair of Neurospora crassa. Nucleic Acids Res. 2005;33:1848-58 pubmed publisher
    ..Evidence is also presented for the two independent pathways for recovery from camptothecin-induced replication fork arrest: one pathway is dependent on QDE3 and MUS50 and the other pathway is dependent on MUS25 and RECQ2...
  79. Bathe F, Hahlen K, Dombi R, Driller L, Schliwa M, Woehlke G. The complex interplay between the neck and hinge domains in kinesin-1 dimerization and motor activity. Mol Biol Cell. 2005;16:3529-37 pubmed publisher
    ..On the basis of yeast two-hybrid data, we propose that the proximal hinge can bind kinesin's cargo-free tail domain and causes inactivation of kinesin by disrupting the neck coiled-coil conformation...
  80. Colot H, Loros J, Dunlap J. Temperature-modulated alternative splicing and promoter use in the Circadian clock gene frequency. Mol Biol Cell. 2005;16:5563-71 pubmed publisher
    ..Evolutionary comparisons with the Sordariaceae reveal conservation of 5' UTR sequences, as well as significant conservation of the alternative splicing events, supporting their relevance to proper regulation of clock function...
  81. Lakin Thomas P. Circadian clock genes frequency and white collar-1 are not essential for entrainment to temperature cycles in Neurospora crassa. Proc Natl Acad Sci U S A. 2006;103:4469-74 pubmed publisher
    ..A single temperature-entrainable oscillator may drive the conidiation rhythm in all strains, and additional properties such as light sensitivity and temperature compensation may be conferred by the intact FRQ/WC loop in the WT strain...
  82. Turner G, Weiss R. Developmental expression of two forms of arginase in Neurospora crassa. Biochim Biophys Acta. 2006;1760:848-57 pubmed publisher
  83. Marx H, Sauer M, Resina D, Vai M, Porro D, Valero F, et al. Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris. FEMS Microbiol Lett. 2006;264:40-7 pubmed publisher
    ..pastoris, human trypsinogen, human serum albumin and Rhizopus oryzae lipase, was evaluated. While the disruption had no effect on the secretion of trypsinogen and albumin, the amount of lipase released from the cells was doubled...
  84. Salgado P, Koivunen M, Makeyev E, Bamford D, Stuart D, Grimes J. The structure of an RNAi polymerase links RNA silencing and transcription. PLoS Biol. 2006;4:e434 pubmed publisher
    ..This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world...
  85. Neiss A, Schafmeier T, Brunner M. Transcriptional regulation and function of the Neurospora clock gene white collar 2 and its isoforms. EMBO Rep. 2008;9:788-94 pubmed publisher
    ..Full-length WC2 and sWC2 are expressed in an antagonistic manner; thus, sWC2 expression seems to be a fail-safe mechanism that maintains total WC2 levels above a threshold...
  86. Estrada A, Youssar L, Scherzinger D, Al Babili S, Avalos J. The ylo-1 gene encodes an aldehyde dehydrogenase responsible for the last reaction in the Neurospora carotenoid pathway. Mol Microbiol. 2008;69:1207-20 pubmed publisher
    ..We conclude that YLO-1 completes the set of enzymes needed for the synthesis of this major Neurospora pigment...
  87. Mehra A, Shi M, Baker C, Colot H, Loros J, Dunlap J. A role for casein kinase 2 in the mechanism underlying circadian temperature compensation. Cell. 2009;137:749-60 pubmed publisher
    ..Finally, mutation of certain putative CK2 phosphosites on FRQ, shown to be phosphorylated in vivo, predictably alters temperature compensation profiles effectively phenocopying CK2 mutants...
  88. Guo J, Cheng P, Liu Y. Functional significance of FRH in regulating the phosphorylation and stability of Neurospora circadian clock protein FRQ. J Biol Chem. 2010;285:11508-15 pubmed publisher
    ..Furthermore, we showed that the rapid FRQ degradation in the absence of FRH is independent of FWD-1, the ubiquitin E3 ligase of FRQ under normal conditions, thus uncovering an alternative pathway for FRQ degradation...
  89. Managadze D, W rtz C, Wiese S, Meyer H, Niehaus G, Erdmann R, et al. A proteomic approach towards the identification of the matrix protein content of the two types of microbodies in Neurospora crassa. Proteomics. 2010;10:3222-34 pubmed publisher
    ..Since the glyoxysomal localization was experimentally demonstrated for one of these enzymes, a new biochemical reaction is expected to be associated with microbody function...
  90. Sun X, Zhang H, Zhang Z, Wang Y, Li S. Involvement of a helix-loop-helix transcription factor CHC-1 in CO(2)-mediated conidiation suppression in Neurospora crassa. Fungal Genet Biol. 2011;48:1077-86 pubmed publisher
    ..In addition, chc-1 also influences expression of genes involved in other important biological processes besides conidiation such as carbon metabolism, sphingolipid synthesis, cell wall synthesis, and calcium signaling...
  91. Carneiro P, Duarte M, Videira A. Disruption of alternative NAD(P)H dehydrogenases leads to decreased mitochondrial ROS in Neurospora crassa. Free Radic Biol Med. 2012;52:402-9 pubmed publisher
    ..This, in addition to a robust increase in scavenging capacity, provides the mutant strain with an improved ability to withstand paraquat treatment...
  92. Maddi A, Fu C, Free S. The Neurospora crassa dfg5 and dcw1 genes encode ?-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall. PLoS ONE. 2012;7:e38872 pubmed publisher
    ..In this model, DFG5 and DCW1 recognize the N-linked galactomannan present on glycoproteins and cross-link it into the cell wall glucan/chitin matrix...